RchiOBHm_Chr4g0396401

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000390
Physical Location & Seq
Reverse (-)
12582887 .. 12583372
486 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36877

Sequence Viewer

Length: 486 bp
ATGAGTGCCAATACCAGCCACAACGCTAAATTTGCATATGGAGTTGGCCTAATAAACCCTTCTAGGGCACCATATCCTAGTTTGGTATATGATCTTGATGGAAAAGACTACGTATATTTTTTGTGTTCACAAGGATATAATGGCAAACTATTGGAAACCATAGCTAGGGACAAGAGTAGCTGCTCATCAAAATCTAATAAAGAAACAGCCAATAACCTAAACTATCCTTCTTTTTCTCTTTCTATCAAGGATCCGAAATTCGTCAATGGGGTCTTCCATAGGACTGTCACTAATTTTGGATCGTTGAATTCCACATACAGTGCTAAAGTGGTGGCTCCATCTGGACTCAAAATCAGTGTGAATCCAAGTGTGCTATCGTTCACATCTCTCAGGCAGAAGAAATCTTTTGTTGTCATAGTAAAAGGGTCGATTGAGAAATCAAATATAGTCTCTGCGTCTTTGGTGTGGGATGATGATGCTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

161

Amino Acids

17.54

Weight (kDa)

9.42

Isoelectric Point (pI)

28.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_6 PF17766 72 - 159 9.7e-22 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 67
AclWI GGATC 3 cut(s) 245, 258, 307
AcsI RAATTY 3 cut(s) 29, 257, 307
AfiI CCNNNNNNNGG 2 cut(s) 64, 165
AgsI TTSAA 1 cut(s) 307
AluBI AGCT 2 cut(s) 164, 180
AluI AGCT 2 cut(s) 164, 180
Alw26I GTCTC 1 cut(s) 454
AlwI GGATC 3 cut(s) 245, 258, 307
AoxI GGCC 1 cut(s) 46
ApeKI GCWGC 1 cut(s) 180
ApoI RAATTY 3 cut(s) 29, 257, 307
BaeGI GKGCMC 1 cut(s) 70
BamHI GGATCC 1 cut(s) 250
BanI GGYRCC 1 cut(s) 67
BbsI GAAGAC 1 cut(s) 265
BbvI GCAGC 1 cut(s) 167
BccI CCATC 2 cut(s) 92, 346
BcoDI GTCTC 1 cut(s) 454
BfaI CTAG 3 cut(s) 63, 78, 165
BisI GCNGC 1 cut(s) 181
BlsI GCNGC 1 cut(s) 182
BmiI GGNNCC 3 cut(s) 69, 252, 336
BmsI GCATC 1 cut(s) 466
BpiI GAAGAC 1 cut(s) 265
BsaAI YACGTR 1 cut(s) 112
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 165
BseGI GGATG 1 cut(s) 475
BseLI CCNNNNNNNGG 2 cut(s) 64, 165
BseMII CTCAG 1 cut(s) 403
BseSI GKGCMC 1 cut(s) 70
BseXI GCAGC 1 cut(s) 167
BshFI GGCC 1 cut(s) 48
BshNI GGYRCC 1 cut(s) 67
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 2 cut(s) 64, 165
BsmAI GTCTC 1 cut(s) 454
BsmFI GGGAC 1 cut(s) 182
BsnI GGCC 1 cut(s) 48
Bsp1286I GDGCHC 1 cut(s) 70
Bsp143I GATC 3 cut(s) 91, 250, 299
BspANI GGCC 1 cut(s) 48
BspCNI CTCAG 1 cut(s) 402
BspLI GGNNCC 3 cut(s) 69, 252, 336
BspPI GGATC 3 cut(s) 245, 258, 307
BspT107I GGYRCC 1 cut(s) 67
BssMI GATC 3 cut(s) 91, 250, 299
Bst4CI ACNGT 2 cut(s) 286, 320
BstBAI YACGTR 1 cut(s) 112
BstDEI CTNAG 1 cut(s) 389
BstF5I GGATG 1 cut(s) 475
BstKTI GATC 3 cut(s) 94, 253, 302
BstMAI GTCTC 1 cut(s) 454
BstMBI GATC 3 cut(s) 91, 250, 299
BstMWI GCNNNNNNNGC 1 cut(s) 32
BstSLI GKGCMC 1 cut(s) 70
BstSNI TACGTA 1 cut(s) 112
BstV1I GCAGC 1 cut(s) 167
BstV2I GAAGAC 1 cut(s) 265
BstX2I RGATCY 1 cut(s) 250
BstYI RGATCY 1 cut(s) 250
BsuRI GGCC 1 cut(s) 48
BtsCI GGATG 1 cut(s) 475
BtsIMutI CAGTG 2 cut(s) 325, 361
CseI GACGC 1 cut(s) 444
CviJI RGCY 6 cut(s) 18, 48, 164, 180, 209, 335
CviKI_1 RGCY 6 cut(s) 18, 48, 164, 180, 209, 335
DdeI CTNAG 1 cut(s) 389
DpnI GATC 3 cut(s) 93, 252, 301
DpnII GATC 3 cut(s) 91, 250, 299
Eco105I TACGTA 1 cut(s) 112
EcoRI GAATTC 1 cut(s) 307
FaqI GGGAC 1 cut(s) 182
FauNDI CATATG 1 cut(s) 37
Fnu4HI GCNGC 1 cut(s) 181
FokI GGATG 1 cut(s) 482
Fsp4HI GCNGC 1 cut(s) 181
FspBI CTAG 3 cut(s) 63, 78, 165
GluI GCNGC 1 cut(s) 181
HaeIII GGCC 1 cut(s) 48
HgaI GACGC 1 cut(s) 444
HinfI GANTC 2 cut(s) 345, 361
Hpy166II GTNNAC 2 cut(s) 128, 381
Hpy188I TCNGA 1 cut(s) 255
Hpy188III TCNNGA 2 cut(s) 95, 342
Hpy8I GTNNAC 2 cut(s) 128, 381
HpyAV CCTTC 2 cut(s) 69, 237
HpyCH4III ACNGT 2 cut(s) 286, 320
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 1 cut(s) 35
HpyF10VI GCNNNNNNNGC 1 cut(s) 32
HpyF3I CTNAG 1 cut(s) 389
HpySE526I ACGT 1 cut(s) 111
Kzo9I GATC 3 cut(s) 91, 250, 299
LmnI GCTCC 1 cut(s) 340
LpnPI CCDG 3 cut(s) 28, 327, 376
Lsp1109I GCAGC 1 cut(s) 167
LweI GCATC 1 cut(s) 466
MaeI CTAG 3 cut(s) 63, 78, 165
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 1 cut(s) 286
MalI GATC 3 cut(s) 93, 252, 301
MboI GATC 3 cut(s) 91, 250, 299
MboII GAAGA 2 cut(s) 265, 409
MflI RGATCY 1 cut(s) 250
MhlI GDGCHC 1 cut(s) 70
MluCI AATT 4 cut(s) 29, 257, 292, 307
MlyI GAGTC 1 cut(s) 339
MwoI GCNNNNNNNGC 1 cut(s) 32
NdeI CATATG 1 cut(s) 37
NdeII GATC 3 cut(s) 91, 250, 299
NlaIV GGNNCC 3 cut(s) 69, 252, 336
NmuCI GTSAC 1 cut(s) 286
PfeI GAWTC 1 cut(s) 361
PkrI GCNGC 1 cut(s) 182
PleI GAGTC 1 cut(s) 339
PpsI GAGTC 1 cut(s) 339
Ppu21I YACGTR 1 cut(s) 112
PspN4I GGNNCC 3 cut(s) 69, 252, 336
PsuI RGATCY 1 cut(s) 250
SatI GCNGC 1 cut(s) 181
Sau3AI GATC 3 cut(s) 91, 250, 299
SchI GAGTC 1 cut(s) 339
SduI GDGCHC 1 cut(s) 70
SetI ASST 4 cut(s) 114, 166, 182, 219
SfaNI GCATC 1 cut(s) 466
SnaBI TACGTA 1 cut(s) 112
Sse9I AATT 4 cut(s) 29, 257, 292, 307
SspMI CTAG 3 cut(s) 63, 78, 165
TaaI ACNGT 2 cut(s) 286, 320
TaiI ACGT 1 cut(s) 114
TaqI TCGA 1 cut(s) 428
TasI AATT 4 cut(s) 29, 257, 292, 307
TfiI GAWTC 1 cut(s) 361
TscAI CASTG 2 cut(s) 325, 361
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 1 cut(s) 180
Tsp45I GTSAC 1 cut(s) 286
TspRI CASTG 2 cut(s) 325, 361
XapI RAATTY 3 cut(s) 29, 257, 307
XspI CTAG 3 cut(s) 63, 78, 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.