Rw1G005900

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000390
Physical Location & Seq
Reverse (-)
11920839 .. 11925627
4789 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G005900.1

Sequence Viewer

Length: 2187 bp
ATGGCTCTTCGATGGTTTCTCCTTCTCAGCCTCATTTGCAGTATTCTACTGCTTGTCAATGTTACTCACTCAACTGCTCAGGATACCCGAAAGGCTTATGTTGTGTATATGGGCGACAAGCCAAAGAACGGGGTACCCATATTACCTGATCTTCATGTGAATATGCTACAGGACGTAGTTGACAGCAGCAACATTGATATTGCACATGAAGCTCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATGACCGGAATGGATGGTGTAGTGTCTATCTTCCCAAGCAAACAAAGGAGCGTGAAAACAACAAAGTCATGGAACTTCCTTGGTTTTCCAGAAACAGTTAAGAGAAGCGCCCTTGAAAGGGATATCATCGTCGGTGTGATGGACAGTGGAATTTGGCCAGAGTCGGACAGCTTTAGTGATGCTGGCTTTGGTCCTCCACCTAAGAAATGGAAAGGCAAATGTCAAGGCAATGGAAATCTTACTTGTAACAATAAAATCATTGGAGCACAGTATTACCGTGCTTCTCGATCCTTCGAAGCAGATGTTGAGTCTCCAAGAGACTCAAATGGTCATGGAACTCATACTGCATCAACAGCAGCTGGGAACACAGTGACCAAGGCAAGCCTAAATGGTTTAGGGTTGGGAAAAGCAAGAGGAGGGGTGCCATCAGCACGTATTGCTGTGTACAAAGTATGCTGGAGCAGCACGCCTGCTTGTGAAGATGCTGACGTTTTAGCGGCATTCGATGATGCCATTGCTGACGGTGTCGACATTCTCTCTATTTCCCTCGGGGGCAGTATTCCAGTTGATTATTTAAATGATGCATTTGCAATTGGGTCATTTCATGCTACCAGAAATGGGATATTAGTTTCCATGGCCGCTGGTAACGAAGGTCCGGGAGCAAAAACTGTGACAAACTTTGCACCATGGCAACTTTCTGTGGCTGCTTCCACCATAAACCGCCAATTCATCACCAAGGTTCAATTGGGTAATAACGGTAAAATCTATGAGGGACTTTTACCAAACATATATGACCTCCGGGGTAAATTCTATCCTTTAATTTATGGTGGAGATGCACCCAACAAAAAAGTAGATACCTTAGAAGAAGATTTGATCAAAGATAAAATTGTGCTTTGTGATGGCCCTATCGATGGGGCTGGGGGTGGGGCCATATTGGGTGGTGCAGTAGGAATTGTTCTGATGGGACAAAAAGTCGCCGATGAAGTGCTTGGCCCTCTTCCCGTGCCTGCATCACACGTCGGGTTGGAAGAACATAGTGACATTTACAAATACATAAAATTAACAAGGAACGCAACTGCAACTATTTGGAAAAGTGAAGAGATTAATGATGTATTGGCTCCATATGTGCCCTCCTACTCATCAAGGGGTCCAAATCCGATCAATCCTAACATTCTCAAGCCAGATTTAGCGGCTCCAGGAACTTACATTCTAGCAGCATATCCCCCAAGTGGAGGTGGCAGTAGAGCTGGATACTATTTAGAAACTGGGACATCAATGGCGTGCCCCCATGCTACAGCCGTAGCTGCATACGTCAAATCATTTCACCCTAAATGGTCACCGGCAGCCATCCAATCGGCTCTCATCACTACCGCTAAATCTATGAGTGCCAAAACTAGCCCTGATGCTGAATTTGCATACGGAGCTGGCCTGATAAACCCTTCTAGGGCTCCATATCCTGGTTTGGTGTATGATCTTGATGAACAAGACTACATAGATTTTTTGTGTTCACAAGGATACAGTGGTAAACTATTGAAAGCCATAACCAAGGACAAGACTAGTTGTTCATCAAAATCTAATAATGGAACAACCAATGACCTGAACTATCCTTCTTTTGCACTTTCCATCAAGGATCCAAAATTTGTTAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCGTCGAATTCCACGTACAGAGTTAAAGTGGTGGCTCCATTGGGACTCAAAATCAACGTGAATCCAAATGTGTTATCGTTCACATCTCTCGGGCAAAAGAAATCTTTTGTTGTCACTATAAAAGGGCCGATTGAGAAATCAAATTTAGTCTCTGCGTCTTTGATGTGGGATGATGGTGTTTTCCAAGTCAGGAGCCCCATCATTGTCTATGTTGCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

728

Amino Acids

77.74

Weight (kDa)

8.32

Isoelectric Point (pI)

33.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 112 4.2e-13 Peptidase inhibitor I9
Peptidase_S8 PF00082 135 - 571 8.3e-45 Subtilase family
fn3_6 PF17766 627 - 724 2e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 133
AccB1I GGYRCC 2 cut(s) 133, 706
AccB7I CCANNNNNTGG 1 cut(s) 1742
AccI GTMKAC 1 cut(s) 813
AciI CCGC 5 cut(s) 782, 924, 1006, 1475, 1656
AclWI GGATC 4 cut(s) 567, 1910, 1923, 1972
AcoI YGGCCR 2 cut(s) 440, 921
AcsI RAATTY 6 cut(s) 435, 1091, 1694, 1922, 1972, 2107
AfaI GTAC 3 cut(s) 135, 731, 1982
AfiI CCNNNNNNNGG 9 cut(s) 128, 402, 403, 903, 1196, 1514, 1517, 1729, 1742
AflIII ACRYGT 1 cut(s) 1300
AgsI TTSAA 4 cut(s) 244, 401, 1028, 1819
AhdI GACNNNNNGTC 1 cut(s) 1256
AhlI ACTAGT 1 cut(s) 1841
AjiI CACGTC 1 cut(s) 1303
AjnI CCWGG 2 cut(s) 1480, 1741
AluBI AGCT 7 cut(s) 212, 228, 456, 644, 1532, 1589, 1709
AluI AGCT 7 cut(s) 212, 228, 456, 644, 1532, 1589, 1709
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 3 cut(s) 597, 600, 2119
AlwI GGATC 4 cut(s) 567, 1910, 1923, 1972
AlwNI CAGNNNCTG 1 cut(s) 644
Ama87I CYCGRG 2 cut(s) 833, 2054
AoxI GGCC 7 cut(s) 440, 921, 1186, 1212, 1276, 1711, 2090
ApeKI GCWGC 8 cut(s) 186, 254, 641, 747, 989, 1499, 1589, 1628
ApoI RAATTY 6 cut(s) 435, 1091, 1694, 1922, 1972, 2107
AseI ATTAAT 1 cut(s) 1389
Asp718I GGTACC 1 cut(s) 133
AspLEI GCGC 1 cut(s) 395
AspS9I GGNCC 7 cut(s) 476, 938, 1187, 1212, 1277, 1433, 2090
AsuC2I CCSGG 2 cut(s) 942, 1085
AsuHPI GGTGA 3 cut(s) 1009, 1601, 1614
AsuII TTCGAA 1 cut(s) 579
AvaI CYCGRG 2 cut(s) 833, 2054
AvaII GGWCC 3 cut(s) 476, 938, 1433
BaeGI GKGCMC 2 cut(s) 1416, 1571
BaeI ACNNNNGTAYC 2 cut(s) 1792, 1825
BalI TGGCCA 1 cut(s) 442
BamHI GGATCC 1 cut(s) 1915
BanI GGYRCC 2 cut(s) 133, 706
BanII GRGCYC 2 cut(s) 1735, 2162
BbsI GAAGAC 1 cut(s) 1930
Bbv12I GWGCWC 1 cut(s) 553
BbvI GCAGC 8 cut(s) 198, 241, 653, 759, 976, 1511, 1576, 1640
BceAI ACGGC 1 cut(s) 1568
BciT130I CCWGG 2 cut(s) 1482, 1743
BciVI GTATCC 3 cut(s) 76, 1529, 1793
BclI TGATCA 1 cut(s) 1158
BcnI CCSGG 2 cut(s) 942, 1085
BcoDI GTCTC 3 cut(s) 597, 600, 2119
BcuI ACTAGT 1 cut(s) 1841
BfaI CTAG 4 cut(s) 1496, 1680, 1728, 1842
BfmI CTRYAG 2 cut(s) 167, 1578
BfoI RGCGCY 1 cut(s) 396
BfuI GTATCC 3 cut(s) 76, 1529, 1793
Bme1390I CCNGG 4 cut(s) 942, 1085, 1482, 1743
Bme18I GGWCC 3 cut(s) 476, 938, 1433
BmeRI GACNNNNNGTC 1 cut(s) 1256
BmeT110I CYCGRG 2 cut(s) 833, 2054
BmgBI CACGTC 1 cut(s) 1303
BmgT120I GGNCC 7 cut(s) 476, 938, 1187, 1212, 1277, 1433, 2090
BmrFI CCNGG 4 cut(s) 942, 1085, 1482, 1743
BmrI ACTGGG 1 cut(s) 1560
BmsI GCATC 8 cut(s) 454, 641, 757, 784, 856, 1108, 1304, 1678
BmuI ACTGGG 1 cut(s) 1560
BpiI GAAGAC 1 cut(s) 1930
BpmI CTGGAG 2 cut(s) 763, 1464
Bpu10I CCTNAGC 1 cut(s) 78
Bpu14I TTCGAA 1 cut(s) 579
BpuEI CTTGAG 1 cut(s) 1445
BpuMI CCSGG 2 cut(s) 942, 1085
Bsa29I ATCGAT 1 cut(s) 1194
BsaAI YACGTR 2 cut(s) 719, 1980
BsaJI CCNNGG 8 cut(s) 364, 660, 832, 918, 971, 1020, 1084, 1830
BsaWI WCCGGW 1 cut(s) 290
Bsc4I CCNNNNNNNGG 9 cut(s) 128, 402, 403, 903, 1196, 1514, 1517, 1729, 1742
Bse118I RCCGGY 1 cut(s) 1624
Bse1I ACTGG 2 cut(s) 848, 1555
Bse3DI GCAATG 3 cut(s) 264, 520, 798
BseBI CCWGG 2 cut(s) 1482, 1743
BseCI ATCGAT 1 cut(s) 1194
BseDI CCNNGG 8 cut(s) 364, 660, 832, 918, 971, 1020, 1084, 1830
BseGI GGATG 3 cut(s) 304, 1632, 2140
BseLI CCNNNNNNNGG 9 cut(s) 128, 402, 403, 903, 1196, 1514, 1517, 1729, 1742
BseMI GCAATG 3 cut(s) 264, 520, 798
BseMII CTCAG 2 cut(s) 40, 92
BseNI ACTGG 2 cut(s) 848, 1555
BseRI GAGGAG 1 cut(s) 714
BseSI GKGCMC 2 cut(s) 1416, 1571
BseXI GCAGC 8 cut(s) 198, 241, 653, 759, 976, 1511, 1576, 1640
BseYI CCCAGC 2 cut(s) 644, 1202
BsgI GTGCAG 1 cut(s) 1248
BshFI GGCC 7 cut(s) 442, 923, 1188, 1214, 1278, 1713, 2092
BshNI GGYRCC 2 cut(s) 133, 706
BshVI ATCGAT 1 cut(s) 1194
BsiHKAI GWGCWC 1 cut(s) 553
BsiHKCI CYCGRG 2 cut(s) 833, 2054
BsiSI CCGG 4 cut(s) 291, 941, 1084, 1625
BslFI GGGAC 4 cut(s) 1071, 1263, 1567, 2022
BslI CCNNNNNNNGG 9 cut(s) 128, 402, 403, 903, 1196, 1514, 1517, 1729, 1742
BsmAI GTCTC 3 cut(s) 597, 600, 2119
BsmFI GGGAC 4 cut(s) 1071, 1263, 1567, 2022
BsmI GAATGC 1 cut(s) 785
BsnI GGCC 7 cut(s) 442, 923, 1188, 1214, 1278, 1713, 2092
BsoBI CYCGRG 2 cut(s) 833, 2054
Bsp119I TTCGAA 1 cut(s) 579
Bsp1286I GDGCHC 5 cut(s) 553, 1416, 1571, 1735, 2162
Bsp1407I TGTACA 1 cut(s) 729
Bsp143I GATC 7 cut(s) 148, 572, 1158, 1443, 1756, 1915, 1964
Bsp19I CCATGG 2 cut(s) 918, 971
BspACI CCGC 5 cut(s) 782, 924, 1006, 1475, 1656
BspANI GGCC 7 cut(s) 442, 923, 1188, 1214, 1278, 1713, 2092
BspCNI CTCAG 2 cut(s) 39, 91
BspDI ATCGAT 1 cut(s) 1194
BspPI GGATC 4 cut(s) 567, 1910, 1923, 1972
BspQI GCTCTTC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 579
BspT107I GGYRCC 2 cut(s) 133, 706
BsrDI GCAATG 3 cut(s) 264, 520, 798
BsrFI RCCGGY 1 cut(s) 1624
BsrGI TGTACA 1 cut(s) 729
BsrI ACTGG 2 cut(s) 848, 1555
BssAI RCCGGY 1 cut(s) 1624
BssECI CCNNGG 8 cut(s) 364, 660, 832, 918, 971, 1020, 1084, 1830
BssMI GATC 7 cut(s) 148, 572, 1158, 1443, 1756, 1915, 1964
BssT1I CCWWGG 6 cut(s) 364, 660, 918, 971, 1020, 1830
Bst2UI CCWGG 2 cut(s) 1482, 1743
Bst6I CTCTTC 3 cut(s) 12, 1287, 1377
BstAPI GCANNNNNTGC 1 cut(s) 722
BstAUI TGTACA 1 cut(s) 729
BstBAI YACGTR 2 cut(s) 719, 1980
BstBI TTCGAA 1 cut(s) 579
BstC8I GCNNGC 7 cut(s) 469, 667, 752, 756, 1293, 1567, 1711
BstDEI CTNAG 4 cut(s) 26, 78, 486, 1144
BstDSI CCRYGG 2 cut(s) 918, 971
BstEII GGTNACC 1 cut(s) 1620
BstF5I GGATG 3 cut(s) 304, 1632, 2140
BstH2I RGCGCY 1 cut(s) 396
BstHHI GCGC 1 cut(s) 395
BstKTI GATC 7 cut(s) 151, 575, 1161, 1446, 1759, 1918, 1967
BstMAI GTCTC 3 cut(s) 597, 600, 2119
BstMBI GATC 7 cut(s) 148, 572, 1158, 1443, 1756, 1915, 1964
BstMWI GCNNNNNNNGC 9 cut(s) 36, 209, 268, 638, 722, 747, 1589, 1697, 1706
BstNI CCWGG 2 cut(s) 1482, 1743
BstPI GGTNACC 1 cut(s) 1620
BstSCI CCNGG 4 cut(s) 940, 1083, 1480, 1741
BstSFI CTRYAG 2 cut(s) 167, 1578
BstSLI GKGCMC 2 cut(s) 1416, 1571
BstV1I GCAGC 8 cut(s) 198, 241, 653, 759, 976, 1511, 1576, 1640
BstV2I GAAGAC 1 cut(s) 1930
BstX2I RGATCY 1 cut(s) 1915
BstYI RGATCY 1 cut(s) 1915
Bsu15I ATCGAT 1 cut(s) 1194
BsuI GTATCC 3 cut(s) 76, 1529, 1793
BsuRI GGCC 7 cut(s) 442, 923, 1188, 1214, 1278, 1713, 2092
BsuTUI ATCGAT 1 cut(s) 1194
BtgI CCRYGG 2 cut(s) 918, 971
BtrI CACGTC 1 cut(s) 1303
BtsCI GGATG 3 cut(s) 304, 1632, 2140
BtsIMutI CAGTG 3 cut(s) 436, 660, 1810
Cac8I GCNNGC 7 cut(s) 469, 667, 752, 756, 1293, 1567, 1711
CaiI CAGNNNCTG 1 cut(s) 644
CfoI GCGC 1 cut(s) 395
Cfr10I RCCGGY 1 cut(s) 1624
Cfr13I GGNCC 7 cut(s) 476, 938, 1187, 1212, 1277, 1433, 2090
ClaI ATCGAT 1 cut(s) 1194
CseI GACGC 1 cut(s) 2109
Csp6I GTAC 3 cut(s) 134, 730, 1981
CviAII CATG 8 cut(s) 155, 206, 354, 617, 890, 919, 972, 1574
CviQI GTAC 3 cut(s) 134, 730, 1981
DdeI CTNAG 4 cut(s) 26, 78, 486, 1144
DpnI GATC 7 cut(s) 150, 574, 1160, 1445, 1758, 1917, 1966
DpnII GATC 7 cut(s) 148, 572, 1158, 1443, 1756, 1915, 1964
DraI TTTAAA 1 cut(s) 861
DriI GACNNNNNGTC 1 cut(s) 1256
EaeI YGGCCR 2 cut(s) 440, 921
Eam1104I CTCTTC 3 cut(s) 12, 1287, 1377
Eam1105I GACNNNNNGTC 1 cut(s) 1256
EarI CTCTTC 3 cut(s) 12, 1287, 1377
Eco130I CCWWGG 6 cut(s) 364, 660, 918, 971, 1020, 1830
Eco24I GRGCYC 2 cut(s) 1735, 2162
Eco32I GATATC 1 cut(s) 409
Eco47I GGWCC 3 cut(s) 476, 938, 1433
Eco88I CYCGRG 2 cut(s) 833, 2054
Eco91I GGTNACC 1 cut(s) 1620
EcoO65I GGTNACC 1 cut(s) 1620
EcoRI GAATTC 1 cut(s) 1972
EcoRII CCWGG 2 cut(s) 1480, 1741
EcoRV GATATC 1 cut(s) 409
EcoT14I CCWWGG 6 cut(s) 364, 660, 918, 971, 1020, 1830
EcoT22I ATGCAT 1 cut(s) 871
EcoT38I GRGCYC 2 cut(s) 1735, 2162
ErhI CCWWGG 6 cut(s) 364, 660, 918, 971, 1020, 1830
FaeI CATG 8 cut(s) 158, 209, 357, 620, 893, 922, 975, 1577
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FaqI GGGAC 4 cut(s) 1071, 1263, 1567, 2022
FatI CATG 8 cut(s) 154, 205, 353, 616, 889, 918, 971, 1573
FauNDI CATATG 1 cut(s) 1408
FbaI TGATCA 1 cut(s) 1158
FblI GTMKAC 1 cut(s) 813
FokI GGATG 3 cut(s) 311, 1619, 2147
FriOI GRGCYC 2 cut(s) 1735, 2162
FspBI CTAG 4 cut(s) 1496, 1680, 1728, 1842
GlaI GCGC 1 cut(s) 394
GsaI CCCAGC 2 cut(s) 648, 1206
GsuI CTGGAG 2 cut(s) 763, 1464
HaeII RGCGCY 1 cut(s) 396
HaeIII GGCC 7 cut(s) 442, 923, 1188, 1214, 1278, 1713, 2092
HapII CCGG 4 cut(s) 291, 941, 1084, 1625
HgaI GACGC 1 cut(s) 2109
HhaI GCGC 1 cut(s) 395
Hin1II CATG 8 cut(s) 158, 209, 357, 620, 893, 922, 975, 1577
Hin6I GCGC 1 cut(s) 393
HinP1I GCGC 1 cut(s) 393
HincII GTYRAC 2 cut(s) 181, 814
HindII GTYRAC 2 cut(s) 181, 814
HinfI GANTC 5 cut(s) 446, 593, 605, 2010, 2026
HpaII CCGG 4 cut(s) 291, 941, 1084, 1625
HphI GGTGA 3 cut(s) 1009, 1601, 1614
Hpy166II GTNNAC 6 cut(s) 181, 730, 814, 1793, 1811, 2046
Hpy188I TCNGA 3 cut(s) 451, 1245, 1443
Hpy188III TCNNGA 5 cut(s) 80, 374, 570, 1760, 2155
Hpy8I GTNNAC 6 cut(s) 181, 730, 814, 1793, 1811, 2046
Hpy99I CGWCG 3 cut(s) 419, 1307, 1972
HpyAV CCTTC 5 cut(s) 32, 586, 929, 1734, 1902
HpyCH4IV ACGT 7 cut(s) 174, 718, 774, 1302, 1596, 1979, 2022
HpyF10VI GCNNNNNNNGC 9 cut(s) 36, 209, 268, 638, 722, 747, 1589, 1697, 1706
HpyF3I CTNAG 4 cut(s) 26, 78, 486, 1144
HpySE526I ACGT 7 cut(s) 174, 718, 774, 1302, 1596, 1979, 2022
Hsp92II CATG 8 cut(s) 158, 209, 357, 620, 893, 922, 975, 1577
HspAI GCGC 1 cut(s) 393
KpnI GGTACC 1 cut(s) 137
Ksp22I TGATCA 1 cut(s) 1158
Kzo9I GATC 7 cut(s) 148, 572, 1158, 1443, 1756, 1915, 1964
LguI GCTCTTC 1 cut(s) 12
Lsp1109I GCAGC 8 cut(s) 198, 241, 653, 759, 976, 1511, 1576, 1640
LweI GCATC 8 cut(s) 454, 641, 757, 784, 856, 1108, 1304, 1678
MaeI CTAG 4 cut(s) 1496, 1680, 1728, 1842
MaeII ACGT 7 cut(s) 174, 718, 774, 1302, 1596, 1979, 2022
MaeIII GTNAC 9 cut(s) 61, 530, 655, 929, 955, 1322, 1620, 1951, 2077
MalI GATC 7 cut(s) 150, 574, 1160, 1445, 1758, 1917, 1966
MboI GATC 7 cut(s) 148, 572, 1158, 1443, 1756, 1915, 1964
MfeI CAATTG 2 cut(s) 876, 1028
MflI RGATCY 1 cut(s) 1915
MhlI GDGCHC 5 cut(s) 553, 1416, 1571, 1735, 2162
MlsI TGGCCA 1 cut(s) 442
MluNI TGGCCA 1 cut(s) 442
MlyI GAGTC 4 cut(s) 455, 599, 602, 2004
MmeI TCCRAC 3 cut(s) 429, 1290, 1942
Mox20I TGGCCA 1 cut(s) 442
Mph1103I ATGCAT 1 cut(s) 871
MscI TGGCCA 1 cut(s) 442
MseI TTAA 8 cut(s) 384, 860, 1103, 1346, 1389, 1929, 1989, 2185
Msp20I TGGCCA 1 cut(s) 442
MspA1I CMGCKG 2 cut(s) 644, 926
MspI CCGG 4 cut(s) 291, 941, 1084, 1625
MspR9I CCNGG 4 cut(s) 942, 1085, 1482, 1743
MunI CAATTG 2 cut(s) 876, 1028
Mva1269I GAATGC 1 cut(s) 785
MvaI CCWGG 2 cut(s) 1482, 1743
MwoI GCNNNNNNNGC 9 cut(s) 36, 209, 268, 638, 722, 747, 1589, 1697, 1706
NciI CCSGG 2 cut(s) 942, 1085
NcoI CCATGG 2 cut(s) 918, 971
NdeI CATATG 1 cut(s) 1408
NdeII GATC 7 cut(s) 148, 572, 1158, 1443, 1756, 1915, 1964
NlaIII CATG 8 cut(s) 158, 209, 357, 620, 893, 922, 975, 1577
NmuCI GTSAC 6 cut(s) 655, 955, 1322, 1620, 1951, 2077
NsiI ATGCAT 1 cut(s) 871
NspV TTCGAA 1 cut(s) 579
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 1976
PctI GAATGC 1 cut(s) 785
PfeI GAWTC 1 cut(s) 2026
PflFI GACNNNGTC 1 cut(s) 809
PflMI CCANNNNNTGG 1 cut(s) 1742
PfoI TCCNGGA 2 cut(s) 940, 1480
PleI GAGTC 4 cut(s) 454, 599, 601, 2004
PpsI GAGTC 4 cut(s) 454, 599, 601, 2004
Ppu21I YACGTR 2 cut(s) 719, 1980
PshBI ATTAAT 1 cut(s) 1389
Psp6I CCWGG 2 cut(s) 1480, 1741
PspEI GGTNACC 1 cut(s) 1620
PspFI CCCAGC 2 cut(s) 644, 1202
PspGI CCWGG 2 cut(s) 1480, 1741
PspPI GGNCC 7 cut(s) 476, 938, 1187, 1212, 1277, 1433, 2090
PsrI GAACNNNNNNTAC 2 cut(s) 1224, 1256
PstNI CAGNNNCTG 1 cut(s) 644
PsuI RGATCY 1 cut(s) 1915
PsyI GACNNNGTC 1 cut(s) 809
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 3 cut(s) 135, 731, 1982
RsaNI GTAC 3 cut(s) 134, 730, 1981
SalI GTCGAC 1 cut(s) 812
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 8 cut(s) 384, 860, 1103, 1346, 1389, 1929, 1989, 2185
Sau3AI GATC 7 cut(s) 148, 572, 1158, 1443, 1756, 1915, 1964
Sau96I GGNCC 7 cut(s) 476, 938, 1187, 1212, 1277, 1433, 2090
SchI GAGTC 4 cut(s) 455, 599, 602, 2004
ScrFI CCNGG 4 cut(s) 942, 1085, 1482, 1743
SduI GDGCHC 5 cut(s) 553, 1416, 1571, 1735, 2162
SfaNI GCATC 8 cut(s) 454, 641, 757, 784, 856, 1108, 1304, 1678
SfcI CTRYAG 2 cut(s) 167, 1578
SfuI TTCGAA 1 cut(s) 579
SinI GGWCC 3 cut(s) 476, 938, 1433
SmiI ATTTAAAT 1 cut(s) 861
SmlI CTYRAG 1 cut(s) 1460
SmoI CTYRAG 1 cut(s) 1460
SpeI ACTAGT 1 cut(s) 1841
SsiI CCGC 5 cut(s) 782, 924, 1006, 1475, 1656
SspMI CTAG 4 cut(s) 1496, 1680, 1728, 1842
StyD4I CCNGG 4 cut(s) 940, 1083, 1480, 1741
StyI CCWWGG 6 cut(s) 364, 660, 918, 971, 1020, 1830
SwaI ATTTAAAT 1 cut(s) 861
TaiI ACGT 7 cut(s) 177, 721, 777, 1305, 1599, 1982, 2025
TaqI TCGA 7 cut(s) 10, 571, 579, 789, 813, 1194, 1970
TatI WGTACW 1 cut(s) 729
TauI GCSGC 3 cut(s) 785, 926, 1478
TfiI GAWTC 1 cut(s) 2026
Tru1I TTAA 8 cut(s) 384, 860, 1103, 1346, 1389, 1929, 1989, 2185
Tru9I TTAA 8 cut(s) 384, 860, 1103, 1346, 1389, 1929, 1989, 2185
TscAI CASTG 3 cut(s) 436, 660, 1810
TseFI GTSAC 6 cut(s) 655, 955, 1322, 1620, 1951, 2077
TseI GCWGC 8 cut(s) 186, 254, 641, 747, 989, 1499, 1589, 1628
Tsp45I GTSAC 6 cut(s) 655, 955, 1322, 1620, 1951, 2077
TspDTI ATGAA 7 cut(s) 143, 222, 878, 1003, 1281, 1779, 1839
TspGWI ACGGA 1 cut(s) 1719
TspRI CASTG 3 cut(s) 436, 660, 1810
Tth111I GACNNNGTC 1 cut(s) 809
Van91I CCANNNNNTGG 1 cut(s) 1742
VpaK11BI GGWCC 3 cut(s) 476, 938, 1433
VspI ATTAAT 1 cut(s) 1389
XapI RAATTY 6 cut(s) 435, 1091, 1694, 1922, 1972, 2107
XcmI CCANNNNNNNNNTGG 2 cut(s) 489, 1027
XmiI GTMKAC 1 cut(s) 813
XspI CTAG 4 cut(s) 1496, 1680, 1728, 1842
Zsp2I ATGCAT 1 cut(s) 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.