Rroxscaffold_4G00287770

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000390
Physical Location & Seq
Forward (+)
8730850 .. 8736803
5954 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00287770.1

Sequence Viewer

Length: 1359 bp
ATGGCTCTTCGATGGTTTCTCCTCCTTAGCCTCATTGGCAGTATTCTATTTCTTGTTGATGTTACTCACTCAGCTGCTCGGGATGCCCGAAAGGCTTATATTGTGTATATGGGTGACAAGCCAAAGAACGGGATGCCAGTAACACCTGATCTTCATGTAAATATTCTACGAGACATAGTTGATAGCAGCATTAATAAGGATATTGCATATGAAGCTCTGCTTCTTCACAGCTACAAGAGAAGTTTCCATGGATTTGCTGCAATGCTCACAGAACAAGAAGCACAAAAACTAGCTGGAATGGATGGTGTAGTGTCTGTCTTTCCAAGTAAAAAGGTGATCGTCAAAACAACAACGTCATGGAATTTCGTTGGGTTTCCAGAATCAATTGGGAGCGGCCCCTTTGAAAGTGATATCATTATCGGTGTGCTCGACAGTGGAATTTGGCCGGAGTTGGACAGCTTTAATGATGTTGGTTTTGGTCCTCCACCTAAGAAATGGAAAGGCATATGTCAAGGCAATAACAACTTCACTTGTAACAATAAAATCATCGGAGCACGGTATTACAAAGCTAGTGACTTCGAAGAAGAAGATATTCAGTCTCCAAGAGATGCATCCGGTCATGGAACCCACTGCGCGTCAAAAGCAGCTGGGAATGCAGTGAGCAACGCAAGCATAAATGGTTTAGGGTTGGGAACAGCAAGAGGAGCGGTGCCATCAGCACGTTTTGCGGTGTACAAAATATGTTGGGACAAAGGTTGTGGTGTTGCTGAGTTTCTAGCGGCAATCGACGATGCCATTGCTGACGGTGTCGACATTATCAGTGCTTCCACTGGAGGCGCTGCGCCAGCTGACTTTTTGAATGACCCATTTGCAATTGGGGCATTTCATGCTACCGTAAATGGGATATTGGTTTCTATGGCCGCTGGTAATGAAGGTCCGCAAAAAAAAAAACTGTGGAAAACATTGCACCATGGCAACTTTCTGTGGCTGCTACCACCACAAACCGCCAGTTCATCACCAAGGTTCAATTGGGTAATGGTAAAATCTATGAGGTTTTGTGTCGGAGATAGCTTAGATGCAGATAAGATCAAAGGTAAAATTGTGCTTTGTGATCGCCCAGATAAGGATGGGAAGGGGGCTGGGGATGGTGCCAAATGGTGGAATGCTTCAATGGGAGTTATTCTGACGGGCAAACAAGTTGTCGAGGAAACCGAATATGCTGGTAACCCTTGTGCAGCATCTTACGTCAATTTAGAAGATAATAGCAACATTTACAAATACATAAACAAACAAGGAAGGCTACTCTCGAGTGGAATGCTACCTGCACCCACGAAGCTACGGTCAAAGAGCTTTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

452

Amino Acids

48.69

Weight (kDa)

8.01

Isoelectric Point (pI)

32.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 112 9e-16 Peptidase inhibitor I9
Peptidase_S8 PF00082 136 - 326 1.4e-20 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1330
AccB1I GGYRCC 2 cut(s) 709, 1148
AccB7I CCANNNNNTGG 1 cut(s) 1158
AccBSI CCGCTC 2 cut(s) 393, 707
AccI GTMKAC 1 cut(s) 810
AccII CGCG 1 cut(s) 635
AciI CCGC 7 cut(s) 393, 707, 728, 779, 921, 938, 1005
AcoI YGGCCR 2 cut(s) 443, 918
AcsI RAATTY 2 cut(s) 361, 438
AfaI GTAC 1 cut(s) 734
AfiI CCNNNNNNNGG 4 cut(s) 128, 900, 1123, 1158
AgsI TTSAA 4 cut(s) 404, 859, 1027, 1170
Alw21I GWGCWC 2 cut(s) 429, 556
Alw26I GTCTC 2 cut(s) 165, 603
Ama87I CYCGRG 2 cut(s) 78, 1306
AoxI GGCC 3 cut(s) 394, 443, 918
ApeKI GCWGC 7 cut(s) 74, 186, 257, 644, 839, 988, 1235
ApoI RAATTY 2 cut(s) 361, 438
ArsI GACNNNNNNTTYG 4 cut(s) 1185, 1217, 1325, 1357
AseI ATTAAT 1 cut(s) 192
Asp700I GAANNNNTTC 1 cut(s) 591
AspLEI GCGC 3 cut(s) 635, 839, 844
AspS9I GGNCC 3 cut(s) 395, 479, 935
AsuHPI GGTGA 3 cut(s) 125, 346, 1008
AsuII TTCGAA 1 cut(s) 579
AvaI CYCGRG 2 cut(s) 78, 1306
AvaII GGWCC 2 cut(s) 479, 935
BanI GGYRCC 2 cut(s) 709, 1148
Bbv12I GWGCWC 2 cut(s) 429, 556
BbvI GCAGC 7 cut(s) 61, 198, 244, 656, 826, 975, 1247
BccI CCATC 5 cut(s) 6, 296, 721, 1121, 1139
BcgI CGANNNNNNTGC 4 cut(s) 779, 813, 1297, 1331
BcoDI GTCTC 2 cut(s) 165, 603
BfaI CTAG 3 cut(s) 290, 570, 776
BfoI RGCGCY 1 cut(s) 840
BfuAI ACCTGC 1 cut(s) 1330
BglI GCCNNNNNGGC 2 cut(s) 36, 92
Bme18I GGWCC 2 cut(s) 479, 935
BmeT110I CYCGRG 2 cut(s) 78, 1306
BmgT120I GGNCC 3 cut(s) 395, 479, 935
BmiI GGNNCC 4 cut(s) 397, 625, 711, 1150
BmsI GCATC 7 cut(s) 73, 123, 598, 620, 781, 1066, 1247
BpmI CTGGAG 1 cut(s) 852
Bpu10I CCTNAGC 1 cut(s) 26
Bpu14I TTCGAA 1 cut(s) 579
BsaJI CCNNGG 3 cut(s) 247, 970, 1019
BsaWI WCCGGW 1 cut(s) 614
Bsc4I CCNNNNNNNGG 4 cut(s) 128, 900, 1123, 1158
Bse1I ACTGG 3 cut(s) 137, 835, 1008
Bse3DI GCAATG 3 cut(s) 267, 795, 962
BseDI CCNNGG 3 cut(s) 247, 970, 1019
BseGI GGATG 6 cut(s) 88, 138, 307, 611, 1132, 1150
BseLI CCNNNNNNNGG 4 cut(s) 128, 900, 1123, 1158
BseMI GCAATG 3 cut(s) 267, 795, 962
BseMII CTCAG 2 cut(s) 84, 759
BseNI ACTGG 3 cut(s) 137, 835, 1008
BseRI GAGGAG 2 cut(s) 11, 717
BseXI GCAGC 7 cut(s) 61, 198, 244, 656, 826, 975, 1247
BseYI CCCAGC 2 cut(s) 647, 1139
BsgI GTGCAG 2 cut(s) 1254, 1308
Bsh1236I CGCG 1 cut(s) 635
BshFI GGCC 3 cut(s) 396, 445, 920
BshNI GGYRCC 2 cut(s) 709, 1148
BsiHKAI GWGCWC 2 cut(s) 429, 556
BsiHKCI CYCGRG 2 cut(s) 78, 1306
BsiSI CCGG 2 cut(s) 446, 615
BslFI GGGAC 1 cut(s) 761
BslI CCNNNNNNNGG 4 cut(s) 128, 900, 1123, 1158
BsmAI GTCTC 2 cut(s) 165, 603
BsmFI GGGAC 1 cut(s) 761
BsmI GAATGC 3 cut(s) 658, 1168, 1320
BsnI GGCC 3 cut(s) 396, 445, 920
BsoBI CYCGRG 2 cut(s) 78, 1306
Bsp119I TTCGAA 1 cut(s) 579
Bsp1286I GDGCHC 2 cut(s) 429, 556
Bsp1407I TGTACA 1 cut(s) 732
Bsp143I GATC 4 cut(s) 148, 336, 1086, 1111
Bsp19I CCATGG 2 cut(s) 247, 970
BspACI CCGC 7 cut(s) 393, 707, 728, 779, 921, 938, 1005
BspANI GGCC 3 cut(s) 396, 445, 920
BspCNI CTCAG 2 cut(s) 83, 760
BspFNI CGCG 1 cut(s) 635
BspLI GGNNCC 4 cut(s) 397, 625, 711, 1150
BspMI ACCTGC 1 cut(s) 1330
BspQI GCTCTTC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 579
BspT107I GGYRCC 2 cut(s) 709, 1148
BsrBI CCGCTC 2 cut(s) 393, 707
BsrDI GCAATG 3 cut(s) 267, 795, 962
BsrGI TGTACA 1 cut(s) 732
BsrI ACTGG 3 cut(s) 137, 835, 1008
BssECI CCNNGG 3 cut(s) 247, 970, 1019
BssMI GATC 4 cut(s) 148, 336, 1086, 1111
BssT1I CCWWGG 3 cut(s) 247, 970, 1019
Bst4CI ACNGT 6 cut(s) 434, 558, 806, 895, 954, 1341
Bst6I CTCTTC 1 cut(s) 12
BstAPI GCANNNNNTGC 2 cut(s) 725, 887
BstAUI TGTACA 1 cut(s) 732
BstBI TTCGAA 1 cut(s) 579
BstC8I GCNNGC 2 cut(s) 670, 846
BstDEI CTNAG 5 cut(s) 26, 70, 489, 768, 1072
BstDSI CCRYGG 2 cut(s) 247, 970
BstEII GGTNACC 1 cut(s) 1223
BstF5I GGATG 6 cut(s) 88, 138, 307, 611, 1132, 1150
BstFNI CGCG 1 cut(s) 635
BstH2I RGCGCY 1 cut(s) 840
BstHHI GCGC 3 cut(s) 635, 839, 844
BstKTI GATC 4 cut(s) 151, 339, 1089, 1114
BstMAI GTCTC 2 cut(s) 165, 603
BstMBI GATC 4 cut(s) 148, 336, 1086, 1111
BstPI GGTNACC 1 cut(s) 1223
BstUI CGCG 1 cut(s) 635
BstV1I GCAGC 7 cut(s) 61, 198, 244, 656, 826, 975, 1247
BsuRI GGCC 3 cut(s) 396, 445, 920
BtgI CCRYGG 2 cut(s) 247, 970
BtsCI GGATG 6 cut(s) 88, 138, 307, 611, 1132, 1150
BtsI GCAGTG 2 cut(s) 628, 663
BtsIMutI CAGTG 5 cut(s) 439, 628, 663, 826, 828
BveI ACCTGC 1 cut(s) 1330
Cac8I GCNNGC 2 cut(s) 670, 846
CfoI GCGC 3 cut(s) 635, 839, 844
Cfr13I GGNCC 3 cut(s) 395, 479, 935
CseI GACGC 1 cut(s) 624
Csp6I GTAC 1 cut(s) 733
CspCI CAANNNNNGTGG 2 cut(s) 739, 774
CviAII CATG 6 cut(s) 155, 248, 357, 620, 887, 971
CviQI GTAC 1 cut(s) 733
DdeI CTNAG 5 cut(s) 26, 70, 489, 768, 1072
DpnI GATC 4 cut(s) 150, 338, 1088, 1113
DpnII GATC 4 cut(s) 148, 336, 1086, 1111
EaeI YGGCCR 2 cut(s) 443, 918
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco130I CCWWGG 3 cut(s) 247, 970, 1019
Eco32I GATATC 1 cut(s) 412
Eco47I GGWCC 2 cut(s) 479, 935
Eco88I CYCGRG 2 cut(s) 78, 1306
Eco91I GGTNACC 1 cut(s) 1223
EcoO65I GGTNACC 1 cut(s) 1223
EcoRV GATATC 1 cut(s) 412
EcoT14I CCWWGG 3 cut(s) 247, 970, 1019
EcoT22I ATGCAT 1 cut(s) 613
ErhI CCWWGG 3 cut(s) 247, 970, 1019
FaeI CATG 6 cut(s) 158, 251, 360, 623, 890, 974
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FaqI GGGAC 1 cut(s) 761
FatI CATG 6 cut(s) 154, 247, 356, 619, 886, 970
FauNDI CATATG 2 cut(s) 208, 506
FblI GTMKAC 1 cut(s) 810
FokI GGATG 6 cut(s) 95, 145, 314, 598, 1139, 1157
FspBI CTAG 3 cut(s) 290, 570, 776
GlaI GCGC 3 cut(s) 634, 838, 843
GsaI CCCAGC 2 cut(s) 651, 1143
GsuI CTGGAG 1 cut(s) 852
HaeII RGCGCY 1 cut(s) 840
HaeIII GGCC 3 cut(s) 396, 445, 920
HapII CCGG 2 cut(s) 446, 615
HgaI GACGC 1 cut(s) 624
HhaI GCGC 3 cut(s) 635, 839, 844
Hin1II CATG 6 cut(s) 158, 251, 360, 623, 890, 974
Hin6I GCGC 3 cut(s) 633, 837, 842
HinP1I GCGC 3 cut(s) 633, 837, 842
HincII GTYRAC 1 cut(s) 811
HindII GTYRAC 1 cut(s) 811
HinfI GANTC 1 cut(s) 380
HpaII CCGG 2 cut(s) 446, 615
HphI GGTGA 3 cut(s) 125, 346, 1008
Hpy166II GTNNAC 2 cut(s) 733, 811
Hpy188I TCNGA 3 cut(s) 551, 1064, 1185
Hpy188III TCNNGA 3 cut(s) 80, 377, 1306
Hpy8I GTNNAC 2 cut(s) 733, 811
Hpy99I CGWCG 1 cut(s) 791
HpyAV CCTTC 3 cut(s) 926, 1126, 1290
HpyCH4III ACNGT 6 cut(s) 434, 558, 806, 895, 954, 1341
HpyCH4IV ACGT 3 cut(s) 353, 721, 1245
HpyCH4V TGCA 9 cut(s) 206, 260, 611, 656, 872, 967, 1079, 1235, 1325
HpyF3I CTNAG 5 cut(s) 26, 70, 489, 768, 1072
HpySE526I ACGT 3 cut(s) 353, 721, 1245
Hsp92II CATG 6 cut(s) 158, 251, 360, 623, 890, 974
HspAI GCGC 3 cut(s) 633, 837, 842
Kzo9I GATC 4 cut(s) 148, 336, 1086, 1111
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 3 cut(s) 390, 551, 704
Lsp1109I GCAGC 7 cut(s) 61, 198, 244, 656, 826, 975, 1247
LweI GCATC 7 cut(s) 73, 123, 598, 620, 781, 1066, 1247
MaeI CTAG 3 cut(s) 290, 570, 776
MaeII ACGT 3 cut(s) 353, 721, 1245
MaeIII GTNAC 6 cut(s) 61, 113, 139, 533, 572, 1223
MalI GATC 4 cut(s) 150, 338, 1088, 1113
MbiI CCGCTC 2 cut(s) 393, 707
MboI GATC 4 cut(s) 148, 336, 1086, 1111
MboII GAAGA 6 cut(s) 143, 215, 593, 596, 599, 1268
MfeI CAATTG 3 cut(s) 384, 873, 1027
MhlI GDGCHC 2 cut(s) 429, 556
MluCI AATT 7 cut(s) 361, 384, 438, 873, 1027, 1098, 1249
MmeI TCCRAC 2 cut(s) 432, 1042
MnlI CCTC 7 cut(s) 32, 41, 492, 695, 827, 1044, 1198
Mph1103I ATGCAT 1 cut(s) 613
MroXI GAANNNNTTC 1 cut(s) 591
MseI TTAA 2 cut(s) 192, 462
MspA1I CMGCKG 4 cut(s) 74, 647, 848, 923
MspI CCGG 2 cut(s) 446, 615
MteI GCGCNGCGC 1 cut(s) 840
MunI CAATTG 3 cut(s) 384, 873, 1027
Mva1269I GAATGC 3 cut(s) 658, 1168, 1320
MvnI CGCG 1 cut(s) 635
NcoI CCATGG 2 cut(s) 247, 970
NdeI CATATG 2 cut(s) 208, 506
NdeII GATC 4 cut(s) 148, 336, 1086, 1111
NlaIII CATG 6 cut(s) 158, 251, 360, 623, 890, 974
NlaIV GGNNCC 4 cut(s) 397, 625, 711, 1150
NmuCI GTSAC 2 cut(s) 113, 572
NsiI ATGCAT 1 cut(s) 613
NspV TTCGAA 1 cut(s) 579
PaeR7I CTCGAG 1 cut(s) 1306
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 3 cut(s) 85, 426, 1209
PctI GAATGC 3 cut(s) 658, 1168, 1320
PdmI GAANNNNTTC 1 cut(s) 591
PfeI GAWTC 1 cut(s) 380
PflFI GACNNNGTC 1 cut(s) 806
PflMI CCANNNNNTGG 1 cut(s) 1158
PshBI ATTAAT 1 cut(s) 192
PspEI GGTNACC 1 cut(s) 1223
PspFI CCCAGC 2 cut(s) 647, 1139
PspN4I GGNNCC 4 cut(s) 397, 625, 711, 1150
PspPI GGNCC 3 cut(s) 395, 479, 935
PsyI GACNNNGTC 1 cut(s) 806
PvuII CAGCTG 3 cut(s) 74, 647, 848
RsaI GTAC 1 cut(s) 734
RsaNI GTAC 1 cut(s) 733
SalI GTCGAC 1 cut(s) 809
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 2 cut(s) 192, 462
Sau3AI GATC 4 cut(s) 148, 336, 1086, 1111
Sau96I GGNCC 3 cut(s) 395, 479, 935
SduI GDGCHC 2 cut(s) 429, 556
SfaNI GCATC 7 cut(s) 73, 123, 598, 620, 781, 1066, 1247
Sfr274I CTCGAG 1 cut(s) 1306
SfuI TTCGAA 1 cut(s) 579
SinI GGWCC 2 cut(s) 479, 935
SlaI CTCGAG 1 cut(s) 1306
SmlI CTYRAG 1 cut(s) 1306
SmoI CTYRAG 1 cut(s) 1306
Sse9I AATT 7 cut(s) 361, 384, 438, 873, 1027, 1098, 1249
SsiI CCGC 7 cut(s) 393, 707, 728, 779, 921, 938, 1005
SspI AATATT 1 cut(s) 163
SspMI CTAG 3 cut(s) 290, 570, 776
StyI CCWWGG 3 cut(s) 247, 970, 1019
TaaI ACNGT 6 cut(s) 434, 558, 806, 895, 954, 1341
TaiI ACGT 3 cut(s) 356, 724, 1248
TaqI TCGA 7 cut(s) 10, 429, 579, 786, 810, 1203, 1307
TasI AATT 7 cut(s) 361, 384, 438, 873, 1027, 1098, 1249
TatI WGTACW 1 cut(s) 732
TauI GCSGC 3 cut(s) 396, 782, 923
TfiI GAWTC 1 cut(s) 380
Tru1I TTAA 2 cut(s) 192, 462
Tru9I TTAA 2 cut(s) 192, 462
TscAI CASTG 5 cut(s) 439, 635, 663, 826, 835
TseFI GTSAC 2 cut(s) 113, 572
TseI GCWGC 7 cut(s) 74, 186, 257, 644, 839, 988, 1235
Tsp45I GTSAC 2 cut(s) 113, 572
TspDTI ATGAA 5 cut(s) 143, 225, 875, 945, 1002
TspRI CASTG 5 cut(s) 439, 635, 663, 826, 835
Tth111I GACNNNGTC 1 cut(s) 806
Van91I CCANNNNNTGG 1 cut(s) 1158
VpaK11BI GGWCC 2 cut(s) 479, 935
VspI ATTAAT 1 cut(s) 192
XapI RAATTY 2 cut(s) 361, 438
XcmI CCANNNNNNNNNTGG 2 cut(s) 492, 1026
XhoI CTCGAG 1 cut(s) 1306
XmiI GTMKAC 1 cut(s) 810
XmnI GAANNNNTTC 1 cut(s) 591
XspI CTAG 3 cut(s) 290, 570, 776
Zsp2I ATGCAT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.