cucumisin-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 115 |
| Acc36I | ACCTGC | 1 cut(s) | 837 |
| Acc65I | GGTACC | 1 cut(s) | 1523 |
| AccB1I | GGYRCC | 1 cut(s) | 1523 |
| AccI | GTMKAC | 4 cut(s) | 20, 57, 1398, 2082 |
| AccII | CGCG | 1 cut(s) | 1129 |
| AccIII | TCCGGA | 1 cut(s) | 1956 |
| AciI | CCGC | 9 cut(s) | 142, 836, 940, 1060, 1101, 1482, 1670, 1914, 2064 |
| AclWI | GGATC | 6 cut(s) | 308, 321, 606, 1430, 1443, 2070 |
| AcoI | YGGCCR | 2 cut(s) | 1483, 2031 |
| AcsI | RAATTY | 1 cut(s) | 949 |
| AcuI | CTGAAG | 3 cut(s) | 88, 1017, 1316 |
| AdeI | CACNNNGTG | 1 cut(s) | 202 |
| AfaI | GTAC | 6 cut(s) | 298, 395, 1525, 1699, 1927, 2016 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 77, 277, 835 |
| AflIII | ACRYGT | 1 cut(s) | 592 |
| AgsI | TTSAA | 6 cut(s) | 583, 1253, 1690, 1860, 1988, 2048 |
| AjnI | CCWGG | 2 cut(s) | 1492, 2028 |
| Alw21I | GWGCWC | 5 cut(s) | 224, 240, 357, 442, 909 |
| Alw26I | GTCTC | 3 cut(s) | 869, 1352, 1753 |
| AlwI | GGATC | 6 cut(s) | 308, 321, 606, 1430, 1443, 2070 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 111 |
| Ama87I | CYCGRG | 1 cut(s) | 1540 |
| Aor13HI | TCCGGA | 1 cut(s) | 1956 |
| AoxI | GGCC | 4 cut(s) | 1358, 1483, 2031, 2068 |
| ApoI | RAATTY | 1 cut(s) | 949 |
| Asp718I | GGTACC | 1 cut(s) | 1523 |
| AspA2I | CCTAGG | 1 cut(s) | 1363 |
| AspLEI | GCGC | 4 cut(s) | 116, 309, 1056, 1129 |
| AspS9I | GGNCC | 1 cut(s) | 1359 |
| AsuHPI | GGTGA | 9 cut(s) | 92, 245, 656, 805, 1126, 1339, 1427, 1850, 1952 |
| AvaI | CYCGRG | 1 cut(s) | 1540 |
| AvrII | CCTAGG | 1 cut(s) | 1363 |
| AxyI | CCTNAGG | 1 cut(s) | 12 |
| BalI | TGGCCA | 1 cut(s) | 2033 |
| BamHI | GGATCC | 2 cut(s) | 313, 1435 |
| BanI | GGYRCC | 1 cut(s) | 1523 |
| BanII | GRGCYC | 2 cut(s) | 224, 240 |
| BauI | CACGAG | 1 cut(s) | 1736 |
| Bbv12I | GWGCWC | 5 cut(s) | 224, 240, 357, 442, 909 |
| BccI | CCATC | 7 cut(s) | 70, 153, 281, 436, 1634, 1688, 1766 |
| BceAI | ACGGC | 3 cut(s) | 42, 76, 1470 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 947, 981 |
| BciT130I | CCWGG | 2 cut(s) | 1494, 2030 |
| BcoDI | GTCTC | 3 cut(s) | 869, 1352, 1753 |
| BfaI | CTAG | 4 cut(s) | 521, 1364, 1460, 1883 |
| BfmI | CTRYAG | 4 cut(s) | 830, 1243, 1402, 1477 |
| BfoI | RGCGCY | 1 cut(s) | 310 |
| BfuAI | ACCTGC | 1 cut(s) | 837 |
| BglII | AGATCT | 1 cut(s) | 1530 |
| BlnI | CCTAGG | 1 cut(s) | 1363 |
| BlpI | GCTNAGC | 1 cut(s) | 441 |
| Bme1390I | CCNGG | 2 cut(s) | 1494, 2030 |
| BmeT110I | CYCGRG | 1 cut(s) | 1540 |
| BmgT120I | GGNCC | 1 cut(s) | 1359 |
| BmiI | GGNNCC | 5 cut(s) | 315, 1153, 1361, 1437, 1525 |
| BmrFI | CCNGG | 2 cut(s) | 1494, 2030 |
| BmsI | GCATC | 2 cut(s) | 361, 642 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1845 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 29, 61 |
| Bpu10I | CCTNAGC | 1 cut(s) | 272 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 441 |
| BpuEI | CTTGAG | 2 cut(s) | 85, 238 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1535 |
| BsaI | GGTCTC | 1 cut(s) | 869 |
| BsaJI | CCNNGG | 2 cut(s) | 71, 1363 |
| BsaWI | WCCGGW | 4 cut(s) | 155, 1039, 1453, 1956 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1938, 1968 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 77, 277, 835 |
| Bse118I | RCCGGY | 1 cut(s) | 2066 |
| Bse1I | ACTGG | 1 cut(s) | 1823 |
| Bse21I | CCTNAGG | 1 cut(s) | 12 |
| Bse3DI | GCAATG | 1 cut(s) | 335 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1535 |
| BseAI | TCCGGA | 1 cut(s) | 1956 |
| BseBI | CCWGG | 2 cut(s) | 1494, 2030 |
| BseDI | CCNNGG | 2 cut(s) | 71, 1363 |
| BseGI | GGATG | 3 cut(s) | 81, 164, 376 |
| BseJI | GATNNNNATC | 1 cut(s) | 1535 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 77, 277, 835 |
| BseMI | GCAATG | 1 cut(s) | 335 |
| BseMII | CTCAG | 5 cut(s) | 26, 198, 263, 455, 1805 |
| BseNI | ACTGG | 1 cut(s) | 1823 |
| BseRI | GAGGAG | 1 cut(s) | 1791 |
| BseX3I | CGGCCG | 1 cut(s) | 1483 |
| BseYI | CCCAGC | 3 cut(s) | 276, 309, 623 |
| BsgI | GTGCAG | 1 cut(s) | 1033 |
| Bsh1236I | CGCG | 1 cut(s) | 1129 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1486 |
| BshFI | GGCC | 4 cut(s) | 1360, 1485, 2033, 2070 |
| BshNI | GGYRCC | 1 cut(s) | 1523 |
| BsiEI | CGRYCG | 1 cut(s) | 1486 |
| BsiHKAI | GWGCWC | 5 cut(s) | 224, 240, 357, 442, 909 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1540 |
| BsiSI | CCGG | 7 cut(s) | 156, 1040, 1149, 1433, 1454, 1957, 2067 |
| BslFI | GGGAC | 2 cut(s) | 293, 1368 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 77, 277, 835 |
| BsmAI | GTCTC | 3 cut(s) | 869, 1352, 1753 |
| BsmFI | GGGAC | 2 cut(s) | 293, 1368 |
| BsnI | GGCC | 4 cut(s) | 1360, 1485, 2033, 2070 |
| Bso31I | GGTCTC | 1 cut(s) | 869 |
| BsoBI | CYCGRG | 1 cut(s) | 1540 |
| Bsp1286I | GDGCHC | 5 cut(s) | 224, 240, 357, 442, 909 |
| Bsp13I | TCCGGA | 1 cut(s) | 1956 |
| Bsp1407I | TGTACA | 1 cut(s) | 393 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 441 |
| BspACI | CCGC | 9 cut(s) | 142, 836, 940, 1060, 1101, 1482, 1670, 1914, 2064 |
| BspANI | GGCC | 4 cut(s) | 1360, 1485, 2033, 2070 |
| BspCNI | CTCAG | 5 cut(s) | 25, 199, 264, 454, 1804 |
| BspEI | TCCGGA | 1 cut(s) | 1956 |
| BspFNI | CGCG | 1 cut(s) | 1129 |
| BspLI | GGNNCC | 5 cut(s) | 315, 1153, 1361, 1437, 1525 |
| BspMAI | CTGCAG | 1 cut(s) | 834 |
| BspMI | ACCTGC | 1 cut(s) | 837 |
| BspPI | GGATC | 6 cut(s) | 308, 321, 606, 1430, 1443, 2070 |
| BspQI | GCTCTTC | 1 cut(s) | 1076 |
| BspT107I | GGYRCC | 1 cut(s) | 1523 |
| BspTNI | GGTCTC | 1 cut(s) | 869 |
| BsrDI | GCAATG | 1 cut(s) | 335 |
| BsrFI | RCCGGY | 1 cut(s) | 2066 |
| BsrGI | TGTACA | 1 cut(s) | 393 |
| BsrI | ACTGG | 1 cut(s) | 1823 |
| BssAI | RCCGGY | 1 cut(s) | 2066 |
| BssECI | CCNNGG | 2 cut(s) | 71, 1363 |
| BssNAI | GTATAC | 1 cut(s) | 1399 |
| BssSI | CACGAG | 1 cut(s) | 1736 |
| BssT1I | CCWWGG | 1 cut(s) | 1363 |
| Bst1107I | GTATAC | 1 cut(s) | 1399 |
| Bst2BI | CACGAG | 1 cut(s) | 1736 |
| Bst2UI | CCWGG | 2 cut(s) | 1494, 2030 |
| Bst4CI | ACNGT | 7 cut(s) | 136, 200, 296, 359, 382, 774, 781 |
| Bst6I | CTCTTC | 1 cut(s) | 1076 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1020 |
| BstAUI | TGTACA | 1 cut(s) | 393 |
| BstDEI | CTNAG | 6 cut(s) | 12, 207, 272, 441, 1193, 1791 |
| BstEII | GGTNACC | 1 cut(s) | 1838 |
| BstF5I | GGATG | 3 cut(s) | 81, 164, 376 |
| BstFNI | CGCG | 1 cut(s) | 1129 |
| BstH2I | RGCGCY | 1 cut(s) | 310 |
| BstHHI | GCGC | 4 cut(s) | 116, 309, 1056, 1129 |
| BstMAI | GTCTC | 3 cut(s) | 869, 1352, 1753 |
| BstMCI | CGRYCG | 1 cut(s) | 1486 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 148, 451, 583, 1020, 1066 |
| BstNI | CCWGG | 2 cut(s) | 1494, 2030 |
| BstNSI | RCATGY | 3 cut(s) | 590, 596, 972 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1845 |
| BstPI | GGTNACC | 1 cut(s) | 1838 |
| BstSCI | CCNGG | 2 cut(s) | 1492, 2028 |
| BstSFI | CTRYAG | 4 cut(s) | 830, 1243, 1402, 1477 |
| BstUI | CGCG | 1 cut(s) | 1129 |
| BstX2I | RGATCY | 4 cut(s) | 313, 598, 1435, 1530 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 716, 860 |
| BstYI | RGATCY | 4 cut(s) | 313, 598, 1435, 1530 |
| BstZ17I | GTATAC | 1 cut(s) | 1399 |
| BstZI | CGGCCG | 1 cut(s) | 1483 |
| Bsu36I | CCTNAGG | 1 cut(s) | 12 |
| BsuRI | GGCC | 4 cut(s) | 1360, 1485, 2033, 2070 |
| BtsCI | GGATG | 3 cut(s) | 81, 164, 376 |
| BtsI | GCAGTG | 1 cut(s) | 793 |
| BtsIMutI | CAGTG | 7 cut(s) | 112, 205, 282, 355, 387, 779, 793 |
| BveI | ACCTGC | 1 cut(s) | 837 |
| CaiI | CAGNNNCTG | 1 cut(s) | 111 |
| CfoI | GCGC | 4 cut(s) | 116, 309, 1056, 1129 |
| Cfr10I | RCCGGY | 1 cut(s) | 2066 |
| Cfr13I | GGNCC | 1 cut(s) | 1359 |
| CseI | GACGC | 2 cut(s) | 644, 785 |
| Csp6I | GTAC | 6 cut(s) | 297, 394, 1524, 1698, 1926, 2015 |
| CviQI | GTAC | 6 cut(s) | 297, 394, 1524, 1698, 1926, 2015 |
| DdeI | CTNAG | 6 cut(s) | 12, 207, 272, 441, 1193, 1791 |
| DraIII | CACNNNGTG | 1 cut(s) | 202 |
| EaeI | YGGCCR | 2 cut(s) | 1483, 2031 |
| EagI | CGGCCG | 1 cut(s) | 1483 |
| Eam1104I | CTCTTC | 1 cut(s) | 1076 |
| EarI | CTCTTC | 1 cut(s) | 1076 |
| EciI | GGCGGA | 1 cut(s) | 2053 |
| Ecl136II | GAGCTC | 2 cut(s) | 222, 238 |
| EclXI | CGGCCG | 1 cut(s) | 1483 |
| Eco130I | CCWWGG | 1 cut(s) | 1363 |
| Eco24I | GRGCYC | 2 cut(s) | 224, 240 |
| Eco31I | GGTCTC | 1 cut(s) | 869 |
| Eco52I | CGGCCG | 1 cut(s) | 1483 |
| Eco53kI | GAGCTC | 2 cut(s) | 222, 238 |
| Eco57I | CTGAAG | 3 cut(s) | 88, 1017, 1316 |
| Eco81I | CCTNAGG | 1 cut(s) | 12 |
| Eco88I | CYCGRG | 1 cut(s) | 1540 |
| Eco91I | GGTNACC | 1 cut(s) | 1838 |
| EcoICRI | GAGCTC | 2 cut(s) | 222, 238 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1359 |
| EcoO65I | GGTNACC | 1 cut(s) | 1838 |
| EcoRII | CCWGG | 2 cut(s) | 1492, 2028 |
| EcoT14I | CCWWGG | 1 cut(s) | 1363 |
| EcoT38I | GRGCYC | 2 cut(s) | 224, 240 |
| ErhI | CCWWGG | 1 cut(s) | 1363 |
| FaqI | GGGAC | 2 cut(s) | 293, 1368 |
| FauI | CCCGC | 3 cut(s) | 829, 1053, 1907 |
| FblI | GTMKAC | 4 cut(s) | 20, 57, 1398, 2082 |
| FokI | GGATG | 3 cut(s) | 88, 171, 383 |
| FriOI | GRGCYC | 2 cut(s) | 224, 240 |
| FspBI | CTAG | 4 cut(s) | 521, 1364, 1460, 1883 |
| FspI | TGCGCA | 1 cut(s) | 115 |
| GlaI | GCGC | 4 cut(s) | 115, 308, 1055, 1128 |
| GsaI | CCCAGC | 3 cut(s) | 280, 313, 627 |
| HaeII | RGCGCY | 1 cut(s) | 310 |
| HaeIII | GGCC | 4 cut(s) | 1360, 1485, 2033, 2070 |
| HapII | CCGG | 7 cut(s) | 156, 1040, 1149, 1433, 1454, 1957, 2067 |
| HgaI | GACGC | 2 cut(s) | 644, 785 |
| HhaI | GCGC | 4 cut(s) | 116, 309, 1056, 1129 |
| Hin6I | GCGC | 4 cut(s) | 114, 307, 1054, 1127 |
| HinP1I | GCGC | 4 cut(s) | 114, 307, 1054, 1127 |
| HincII | GTYRAC | 3 cut(s) | 21, 58, 2083 |
| HindII | GTYRAC | 3 cut(s) | 21, 58, 2083 |
| HinfI | GANTC | 7 cut(s) | 17, 40, 67, 482, 1043, 1559, 1796 |
| HpaII | CCGG | 7 cut(s) | 156, 1040, 1149, 1433, 1454, 1957, 2067 |
| HphI | GGTGA | 9 cut(s) | 92, 245, 656, 805, 1126, 1339, 1427, 1850, 1952 |
| Hpy188I | TCNGA | 6 cut(s) | 72, 261, 528, 768, 958, 2075 |
| Hpy99I | CGWCG | 4 cut(s) | 25, 28, 62, 2084 |
| HpyAV | CCTTC | 6 cut(s) | 1291, 1597, 1666, 1840, 1954, 1959 |
| HpyCH4III | ACNGT | 7 cut(s) | 136, 200, 296, 359, 382, 774, 781 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 148, 451, 583, 1020, 1066 |
| HpyF3I | CTNAG | 6 cut(s) | 12, 207, 272, 441, 1193, 1791 |
| HspAI | GCGC | 4 cut(s) | 114, 307, 1054, 1127 |
| Kpn2I | TCCGGA | 1 cut(s) | 1956 |
| KpnI | GGTACC | 1 cut(s) | 1527 |
| KroI | GCCGGC | 1 cut(s) | 2066 |
| KroNI | GCCGGC | 1 cut(s) | 2068 |
| LguI | GCTCTTC | 1 cut(s) | 1076 |
| LmnI | GCTCC | 6 cut(s) | 50, 227, 977, 1157, 1186, 2031 |
| LweI | GCATC | 2 cut(s) | 361, 642 |
| MaeI | CTAG | 4 cut(s) | 521, 1364, 1460, 1883 |
| MaeIII | GTNAC | 7 cut(s) | 107, 290, 415, 569, 960, 1838, 1996 |
| MboII | GAAGA | 6 cut(s) | 179, 892, 1010, 1063, 1153, 1156 |
| MfeI | CAATTG | 1 cut(s) | 922 |
| MflI | RGATCY | 4 cut(s) | 313, 598, 1435, 1530 |
| MhlI | GDGCHC | 5 cut(s) | 224, 240, 357, 442, 909 |
| MlsI | TGGCCA | 1 cut(s) | 2033 |
| MluCI | AATT | 7 cut(s) | 703, 847, 922, 949, 1119, 1502, 1983 |
| MluNI | TGGCCA | 1 cut(s) | 2033 |
| MlyI | GAGTC | 2 cut(s) | 26, 76 |
| MmeI | TCCRAC | 1 cut(s) | 348 |
| Mox20I | TGGCCA | 1 cut(s) | 2033 |
| MroI | TCCGGA | 1 cut(s) | 1956 |
| MroNI | GCCGGC | 1 cut(s) | 2066 |
| MscI | TGGCCA | 1 cut(s) | 2033 |
| MseI | TTAA | 2 cut(s) | 669, 1815 |
| MslI | CAYNNNNRTG | 2 cut(s) | 591, 1554 |
| Msp20I | TGGCCA | 1 cut(s) | 2033 |
| MspA1I | CMGCKG | 4 cut(s) | 748, 1060, 1098, 1482 |
| MspI | CCGG | 7 cut(s) | 156, 1040, 1149, 1433, 1454, 1957, 2067 |
| MspR9I | CCNGG | 2 cut(s) | 1494, 2030 |
| MunI | CAATTG | 1 cut(s) | 922 |
| MvaI | CCWGG | 2 cut(s) | 1494, 2030 |
| MvnI | CGCG | 1 cut(s) | 1129 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 148, 451, 583, 1020, 1066 |
| NaeI | GCCGGC | 1 cut(s) | 2068 |
| NgoMIV | GCCGGC | 1 cut(s) | 2066 |
| NlaIV | GGNNCC | 5 cut(s) | 315, 1153, 1361, 1437, 1525 |
| NmuCI | GTSAC | 3 cut(s) | 107, 1838, 1996 |
| NsbI | TGCGCA | 1 cut(s) | 115 |
| NspI | RCATGY | 3 cut(s) | 590, 596, 972 |
| PaeI | GCATGC | 2 cut(s) | 590, 972 |
| PaeR7I | CTCGAG | 1 cut(s) | 1540 |
| PciI | ACATGT | 1 cut(s) | 592 |
| PciSI | GCTCTTC | 1 cut(s) | 1076 |
| PdiI | GCCGGC | 1 cut(s) | 2068 |
| PfeI | GAWTC | 5 cut(s) | 40, 482, 1043, 1559, 1796 |
| PleI | GAGTC | 2 cut(s) | 25, 75 |
| PpsI | GAGTC | 2 cut(s) | 25, 75 |
| PscI | ACATGT | 1 cut(s) | 592 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1845 |
| Psp124BI | GAGCTC | 2 cut(s) | 224, 240 |
| Psp6I | CCWGG | 2 cut(s) | 1492, 2028 |
| PspEI | GGTNACC | 1 cut(s) | 1838 |
| PspFI | CCCAGC | 3 cut(s) | 276, 309, 623 |
| PspGI | CCWGG | 2 cut(s) | 1492, 2028 |
| PspN4I | GGNNCC | 5 cut(s) | 315, 1153, 1361, 1437, 1525 |
| PspPI | GGNCC | 1 cut(s) | 1359 |
| PstI | CTGCAG | 1 cut(s) | 834 |
| PstNI | CAGNNNCTG | 1 cut(s) | 111 |
| PsuI | RGATCY | 4 cut(s) | 313, 598, 1435, 1530 |
| PvuII | CAGCTG | 2 cut(s) | 748, 1098 |
| RsaI | GTAC | 6 cut(s) | 298, 395, 1525, 1699, 1927, 2016 |
| RsaNI | GTAC | 6 cut(s) | 297, 394, 1524, 1698, 1926, 2015 |
| RseI | CAYNNNNRTG | 2 cut(s) | 591, 1554 |
| SacI | GAGCTC | 2 cut(s) | 224, 240 |
| SalI | GTCGAC | 3 cut(s) | 19, 56, 2081 |
| SapI | GCTCTTC | 1 cut(s) | 1076 |
| SaqAI | TTAA | 2 cut(s) | 669, 1815 |
| Sau96I | GGNCC | 1 cut(s) | 1359 |
| SbfI | CCTGCAGG | 1 cut(s) | 834 |
| SchI | GAGTC | 2 cut(s) | 26, 76 |
| ScrFI | CCNGG | 2 cut(s) | 1494, 2030 |
| SdaI | CCTGCAGG | 1 cut(s) | 834 |
| SduI | GDGCHC | 5 cut(s) | 224, 240, 357, 442, 909 |
| SfaNI | GCATC | 2 cut(s) | 361, 642 |
| SfcI | CTRYAG | 4 cut(s) | 830, 1243, 1402, 1477 |
| Sfr274I | CTCGAG | 1 cut(s) | 1540 |
| SlaI | CTCGAG | 1 cut(s) | 1540 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 591, 1554 |
| SmlI | CTYRAG | 3 cut(s) | 64, 217, 1540 |
| SmoI | CTYRAG | 3 cut(s) | 64, 217, 1540 |
| SphI | GCATGC | 2 cut(s) | 590, 972 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 834 |
| Sse9I | AATT | 7 cut(s) | 703, 847, 922, 949, 1119, 1502, 1983 |
| SsiI | CCGC | 9 cut(s) | 142, 836, 940, 1060, 1101, 1482, 1670, 1914, 2064 |
| SspI | AATATT | 1 cut(s) | 449 |
| SspMI | CTAG | 4 cut(s) | 521, 1364, 1460, 1883 |
| SstI | GAGCTC | 2 cut(s) | 224, 240 |
| StyD4I | CCNGG | 2 cut(s) | 1492, 2028 |
| StyI | CCWWGG | 1 cut(s) | 1363 |
| TaaI | ACNGT | 7 cut(s) | 136, 200, 296, 359, 382, 774, 781 |
| TasI | AATT | 7 cut(s) | 703, 847, 922, 949, 1119, 1502, 1983 |
| TatI | WGTACW | 1 cut(s) | 393 |
| TauI | GCSGC | 2 cut(s) | 1485, 1673 |
| TfiI | GAWTC | 5 cut(s) | 40, 482, 1043, 1559, 1796 |
| Tru1I | TTAA | 2 cut(s) | 669, 1815 |
| Tru9I | TTAA | 2 cut(s) | 669, 1815 |
| TscAI | CASTG | 7 cut(s) | 112, 205, 289, 362, 387, 779, 793 |
| TseFI | GTSAC | 3 cut(s) | 107, 1838, 1996 |
| Tsp45I | GTSAC | 3 cut(s) | 107, 1838, 1996 |
| TspDTI | ATGAA | 3 cut(s) | 1572, 1648, 2007 |
| TspGWI | ACGGA | 3 cut(s) | 1347, 1711, 2068 |
| TspRI | CASTG | 7 cut(s) | 112, 205, 289, 362, 387, 779, 793 |
| XapI | RAATTY | 1 cut(s) | 949 |
| XceI | RCATGY | 3 cut(s) | 590, 596, 972 |
| XhoI | CTCGAG | 1 cut(s) | 1540 |
| XmaJI | CCTAGG | 1 cut(s) | 1363 |
| XmiI | GTMKAC | 4 cut(s) | 20, 57, 1398, 2082 |
| XspI | CTAG | 4 cut(s) | 521, 1364, 1460, 1883 |