RLG00000009546

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000390
Physical Location & Seq
Forward (+)
54310039 .. 54310718
680 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009546

Sequence Viewer

Length: 405 bp
ATGGCGTGCCTTCCTTCTACGGCCGTAGCTGCATATGTCAAATCATTTCATCCTAAATGGTCACCGGCTGTTATCCAGTTGGCTCTCACCACTACTGGATACAATGGCAAACTATTGGAAACCATAGCTAGGGACAAGAGTTGCTGCTCATCAAAATCTAATAAAGAAACAGCCAATAACCTGAACTACCCTTCTTTTGCTCTTTCTATCAAGGATCCGAAATTGGTCAATGGAGTCTTCTATAGGACTGTCACTAATTTTGGATTGTCGGATTCCACATACAGTGCTAAAGTGGTGGCTCCATCTGGACTCAAAATCAGTGTGAATCCAAGTGTGCTATCGTTCACATCTCTCGGGCAGAAGAAATCTTTTGTTGTCACAGTAAAAGGCCCGATTGAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

135

Amino Acids

14.34

Weight (kDa)

9.71

Isoelectric Point (pI)

27.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_6 PF17766 60 - 129 3.5e-19 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 209, 222
AcoI YGGCCR 1 cut(s) 21
AfiI CCNNNNNNNGG 1 cut(s) 129
AluBI AGCT 2 cut(s) 29, 128
AluI AGCT 2 cut(s) 29, 128
AlwI GGATC 2 cut(s) 209, 222
Ama87I CYCGRG 1 cut(s) 353
AoxI GGCC 2 cut(s) 21, 388
ApeKI GCWGC 2 cut(s) 29, 144
AspS9I GGNCC 1 cut(s) 389
AsuHPI GGTGA 2 cut(s) 54, 79
AvaI CYCGRG 1 cut(s) 353
BamHI GGATCC 1 cut(s) 214
BbsI GAAGAC 1 cut(s) 229
BbvI GCAGC 2 cut(s) 16, 131
BccI CCATC 1 cut(s) 310
BceAI ACGGC 2 cut(s) 8, 36
BciVI GTATCC 1 cut(s) 92
BfaI CTAG 1 cut(s) 129
BfmI CTRYAG 1 cut(s) 241
BfuI GTATCC 1 cut(s) 92
BisI GCNGC 2 cut(s) 30, 145
BlsI GCNGC 2 cut(s) 31, 146
BmeT110I CYCGRG 1 cut(s) 353
BmgT120I GGNCC 1 cut(s) 389
BmiI GGNNCC 2 cut(s) 216, 300
BpiI GAAGAC 1 cut(s) 229
Bsc4I CCNNNNNNNGG 1 cut(s) 129
Bse118I RCCGGY 1 cut(s) 64
Bse1I ACTGG 2 cut(s) 76, 100
BseGI GGATG 1 cut(s) 49
BseLI CCNNNNNNNGG 1 cut(s) 129
BseNI ACTGG 2 cut(s) 76, 100
BseX3I CGGCCG 1 cut(s) 21
BseXI GCAGC 2 cut(s) 16, 131
Bsh1285I CGRYCG 1 cut(s) 24
BshFI GGCC 2 cut(s) 23, 390
BsiEI CGRYCG 1 cut(s) 24
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 1 cut(s) 65
BslFI GGGAC 1 cut(s) 146
BslI CCNNNNNNNGG 1 cut(s) 129
BsmFI GGGAC 1 cut(s) 146
BsnI GGCC 2 cut(s) 23, 390
BsoBI CYCGRG 1 cut(s) 353
Bsp143I GATC 1 cut(s) 214
BspANI GGCC 2 cut(s) 23, 390
BspLI GGNNCC 2 cut(s) 216, 300
BspPI GGATC 2 cut(s) 209, 222
BsrFI RCCGGY 1 cut(s) 64
BsrI ACTGG 2 cut(s) 76, 100
BssAI RCCGGY 1 cut(s) 64
BssMI GATC 1 cut(s) 214
Bst4CI ACNGT 3 cut(s) 250, 284, 382
BstC8I GCNNGC 1 cut(s) 7
BstEII GGTNACC 1 cut(s) 60
BstF5I GGATG 1 cut(s) 49
BstKTI GATC 1 cut(s) 217
BstMBI GATC 1 cut(s) 214
BstMCI CGRYCG 1 cut(s) 24
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstPI GGTNACC 1 cut(s) 60
BstSFI CTRYAG 1 cut(s) 241
BstV1I GCAGC 2 cut(s) 16, 131
BstV2I GAAGAC 1 cut(s) 229
BstX2I RGATCY 1 cut(s) 214
BstYI RGATCY 1 cut(s) 214
BstZI CGGCCG 1 cut(s) 21
BsuI GTATCC 1 cut(s) 92
BsuRI GGCC 2 cut(s) 23, 390
BtsCI GGATG 1 cut(s) 49
BtsIMutI CAGTG 2 cut(s) 289, 325
Cac8I GCNNGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 64
Cfr13I GGNCC 1 cut(s) 389
CviJI RGCY 8 cut(s) 23, 29, 68, 83, 128, 173, 299, 390
CviKI_1 RGCY 8 cut(s) 23, 29, 68, 83, 128, 173, 299, 390
DpnI GATC 1 cut(s) 216
DpnII GATC 1 cut(s) 214
EaeI YGGCCR 1 cut(s) 21
EagI CGGCCG 1 cut(s) 21
EclXI CGGCCG 1 cut(s) 21
Eco52I CGGCCG 1 cut(s) 21
Eco88I CYCGRG 1 cut(s) 353
Eco91I GGTNACC 1 cut(s) 60
EcoO65I GGTNACC 1 cut(s) 60
FaiI YATR 5 cut(s) 34, 36, 125, 243, 280
FaqI GGGAC 1 cut(s) 146
FauNDI CATATG 1 cut(s) 34
Fnu4HI GCNGC 2 cut(s) 30, 145
FokI GGATG 1 cut(s) 36
Fsp4HI GCNGC 2 cut(s) 30, 145
FspBI CTAG 1 cut(s) 129
GluI GCNGC 2 cut(s) 30, 145
HaeIII GGCC 2 cut(s) 23, 390
HapII CCGG 1 cut(s) 65
HinfI GANTC 4 cut(s) 234, 272, 309, 325
HpaII CCGG 1 cut(s) 65
HphI GGTGA 2 cut(s) 54, 79
Hpy166II GTNNAC 1 cut(s) 345
Hpy188I TCNGA 2 cut(s) 219, 271
Hpy188III TCNNGA 1 cut(s) 306
Hpy8I GTNNAC 1 cut(s) 345
HpyAV CCTTC 3 cut(s) 20, 24, 201
HpyCH4III ACNGT 3 cut(s) 250, 284, 382
HpyCH4V TGCA 1 cut(s) 32
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
Kzo9I GATC 1 cut(s) 214
LmnI GCTCC 1 cut(s) 304
LpnPI CCDG 5 cut(s) 78, 81, 89, 194, 291
Lsp1109I GCAGC 2 cut(s) 16, 131
MaeI CTAG 1 cut(s) 129
MaeIII GTNAC 3 cut(s) 60, 250, 376
MalI GATC 1 cut(s) 216
MboI GATC 1 cut(s) 214
MboII GAAGA 2 cut(s) 229, 373
MflI RGATCY 1 cut(s) 214
MluCI AATT 2 cut(s) 221, 256
MlyI GAGTC 2 cut(s) 243, 303
MmeI TCCRAC 1 cut(s) 249
MspI CCGG 1 cut(s) 65
MwoI GCNNNNNNNGC 1 cut(s) 29
NdeI CATATG 1 cut(s) 34
NdeII GATC 1 cut(s) 214
NlaIV GGNNCC 2 cut(s) 216, 300
NmuCI GTSAC 3 cut(s) 60, 250, 376
PfeI GAWTC 2 cut(s) 272, 325
PkrI GCNGC 2 cut(s) 31, 146
PleI GAGTC 2 cut(s) 242, 303
PpsI GAGTC 2 cut(s) 242, 303
PspEI GGTNACC 1 cut(s) 60
PspN4I GGNNCC 2 cut(s) 216, 300
PspPI GGNCC 1 cut(s) 389
PsuI RGATCY 1 cut(s) 214
SatI GCNGC 2 cut(s) 30, 145
Sau3AI GATC 1 cut(s) 214
Sau96I GGNCC 1 cut(s) 389
SchI GAGTC 2 cut(s) 243, 303
SetI ASST 3 cut(s) 31, 130, 183
SfcI CTRYAG 1 cut(s) 241
Sse9I AATT 2 cut(s) 221, 256
SspMI CTAG 1 cut(s) 129
TaaI ACNGT 3 cut(s) 250, 284, 382
TasI AATT 2 cut(s) 221, 256
TfiI GAWTC 2 cut(s) 272, 325
TscAI CASTG 2 cut(s) 289, 325
TseFI GTSAC 3 cut(s) 60, 250, 376
TseI GCWGC 2 cut(s) 29, 144
Tsp45I GTSAC 3 cut(s) 60, 250, 376
TspDTI ATGAA 1 cut(s) 38
TspRI CASTG 2 cut(s) 289, 325
XspI CTAG 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.