RLG00000028909

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000390
Physical Location & Seq
Forward (+)
30137736 .. 30141046
3311 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028909

Sequence Viewer

Length: 2232 bp
ATGGCTCTTCGATGGTTTCTCTTTCTTAGCCTCATTAGTAGTATTGTACTTCTTGCTCATGTTACTCACTCAGTTGCTCAGGATGCCCGAAAGGCTTATATTGTGTATATGGGTGACAAGCCAAAGAAAGGGGTGCTAGTAACACCTAATCATCATGTGAATATTCTACGAGACGTAGTTGACAACAACATTAATAATGATATTGCACATGAAGCTCTTCTTCTTCACAGCTACAAGAGAAGTTTCCATGGATTTGCTGCAATGCTCACAGAACAAGAAGCACAAAAACTGGATGGAATGGATGGTGTAGTGTCTGTCTTCCCAAGCAAACAAATGAGCATCAAAACAACAAAGTCATGGAACTTCCTTGGGTTTCCAGAAACAGTTAAAAGAAGCGACCTTGAAAAAGATATCATCATCGGTGTGATCGACACTGGAATTTGGCCAGAATCACGCAGCTTTAGCGATGTTGGTTTTGGTCCTCCACCCAAGAAATGGAAAGGCATATGTCAAGGCAATAAAAACTTTACTTGTAACAATAAAATTATCGGAGCACGGTATTACCGTCATTCGGGATCCTTTGAAGAAGAACTTGAATCTCCAAGAGACTCTGAAGGTCATGGAACCCATTGCGCATCAATAGCAGCTGGGAACATAGTGAGAAATACGAGCATAAATGGTTTAGGATTTGGAAACGCAAGAGGAGGGGTACCATCAGCACATATTGCGGTGTACAAAGCATGCTGGAAGAACGGTTGTTCAGATGCTGACATTTTAGCGGCATTCGATGACGCCGTTGCTGACGGTGTCGATATAATCTCTGCTTCCATTGCAAGCAAAGTTCCAAACGATTATTTTCGAAACGGAGTTGCAATTGGGGCATTTCACGCTACCAGAAAGGGAATACTAGTTTCAACTTGCAGTGGTAACGAAGGTCCGAAAAAAAGAACTGTGATAAACTTTGCACCGTGGATGCTTTCTGTGGCTGCTACCACCATAAACCGTCAGTTCATCACCAAAGTTCAATTGGGTAACGGAAAAGTCTATGAGGGACTATTACCAAACATACATGACCTCGAGGGTAAATTCTATCCTTTAATATATGGTGGAGACGCACCTAATACAACAGAAGGTGGTAACGCGACTAAGTCAAGGTTCTGTTCCACAAATAGCTTAAAAGATGATTTGATCAGAGGCAAAATTGTGCTTTGCGATAGAGGCCCCGATGGCAGTGGGGATGGGTATGGGGCCATAGAGGGTTATGCAACCGGAGTTATTCTGACGGGCGTAAAAGTTGCCGAGGAGCTGGTTGGCCCTTTTCCCCTGCCTGCATCTCACGTCGGATTGGAAGAAAACAGCAAAATTTACAAATACATAAGCGAAACAAGGAACCCAATTGCAACTATTTTCAAAAGTGAAGAGATTAATGATGTATTGGCTCCATATGTGCCCTCCTACTCGTCAAGGGGTCCAAATCCGATGAATCCTAACATTCTCAAGCCAGATTTAGCAGCTCCAGGTACTCATATTCTAGCAGCATATAATCACCAACTGGACCAACTATCAGTTGGTGATGGCAGAATTGAATATAATTTGGATAGTGGGACATCAATGGCTTGCCCTCATGCTGCAGGCGTAGCTGCATACGTCAAATCATTTCACTCTAAATGGTCACCGGCTGCTATTCAATCGGCTCTCATCACTACAGCTAAACCTATGAGTGCCAAAACTAGCCCGCACGCTGAATTTGCATATGGAGCTGGCCTACTAAACCCTTCTAGGGCTCCATATCCTGGTTTGGTATACGATCTTGATGAACAAGACTACTTATATTTTTTATGTTCACAAGGATATAGTGGCAAACTTTTGGAAATCATAACCAGGGACAACTGCTCATCAAAATCTAATAATGAAACAGCCAATGACCTGAACTATCCTTCTTTTGCTCTTTCAATCAAGGATCCAGAATTTATCAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCGTCAAATTCCACATACAGAGCTAAAGTGGTAGCTCCATCTGGACTCAAAATCAATGTGAATCCAAATGTGCTATCATTCACATCTCTCAGCCAGAAGAAATCTTTTGTTGTGACGATAAAAGGGTCGATTGAGAAATCAAATATAGTTTCTGCATCTTTGGTGTGGGATAATGGTGATTTTCAAGTCAGGAGCCCGATTGTTGTCTATGTTACAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

744

Amino Acids

80.41

Weight (kDa)

8.12

Isoelectric Point (pI)

36.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 114 6.3e-15 Peptidase inhibitor I9
Peptidase_S8 PF00082 136 - 588 1.4e-44 Subtilase family
fn3_6 PF17766 642 - 739 9.2e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1076
Acc16I TGCGCA 1 cut(s) 634
Acc65I GGTACC 1 cut(s) 709
AccB1I GGYRCC 1 cut(s) 709
AccB7I CCANNNNNTGG 2 cut(s) 495, 1793
AccI GTMKAC 1 cut(s) 1803
AccII CGCG 1 cut(s) 1142
AciI CCGC 3 cut(s) 728, 779, 1736
AclWI GGATC 5 cut(s) 570, 583, 1955, 1968, 2017
AcoI YGGCCR 1 cut(s) 443
AcsI RAATTY 6 cut(s) 438, 1085, 1362, 1745, 1967, 2017
AcuI CTGAAG 1 cut(s) 633
AcyI GRCGYC 1 cut(s) 792
AfaI GTAC 4 cut(s) 48, 711, 734, 1522
AfiI CCNNNNNNNGG 5 cut(s) 128, 495, 571, 1780, 1793
AhlI ACTAGT 1 cut(s) 907
AjiI CACGTC 1 cut(s) 1339
AjnI CCWGG 3 cut(s) 1516, 1792, 1880
Alw21I GWGCWC 1 cut(s) 556
Alw26I GTCTC 3 cut(s) 165, 600, 1104
AlwI GGATC 5 cut(s) 570, 583, 1955, 1968, 2017
AlwNI CAGNNNCTG 1 cut(s) 767
Ama87I CYCGRG 1 cut(s) 1076
AoxI GGCC 5 cut(s) 443, 1219, 1248, 1312, 1762
ApeKI GCWGC 9 cut(s) 257, 456, 644, 986, 1511, 1535, 1628, 1640, 1679
ApoI RAATTY 6 cut(s) 438, 1085, 1362, 1745, 1967, 2017
AseI ATTAAT 2 cut(s) 192, 1425
Asp700I GAANNNNTTC 1 cut(s) 216
Asp718I GGTACC 1 cut(s) 709
AspLEI GCGC 1 cut(s) 635
AspS9I GGNCC 7 cut(s) 479, 935, 1220, 1248, 1313, 1469, 1555
AsuHPI GGTGA 6 cut(s) 125, 1006, 1538, 1583, 1665, 2198
AsuII TTCGAA 1 cut(s) 859
AvaI CYCGRG 1 cut(s) 1076
AvaII GGWCC 4 cut(s) 479, 935, 1469, 1555
BaeGI GKGCMC 1 cut(s) 1452
BalI TGGCCA 1 cut(s) 445
BamHI GGATCC 2 cut(s) 575, 1960
BanI GGYRCC 1 cut(s) 709
BanII GRGCYC 2 cut(s) 1786, 2207
BbsI GAAGAC 2 cut(s) 310, 1975
Bbv12I GWGCWC 1 cut(s) 556
BbvI GCAGC 9 cut(s) 244, 468, 656, 973, 1523, 1547, 1615, 1627, 1666
BccI CCATC 8 cut(s) 6, 287, 296, 721, 1220, 1232, 1568, 2056
BceAI ACGGC 1 cut(s) 779
BciT130I CCWGG 3 cut(s) 1518, 1794, 1882
BclI TGATCA 1 cut(s) 1188
BcoDI GTCTC 3 cut(s) 165, 600, 1104
BcuI ACTAGT 1 cut(s) 907
BfaI CTAG 5 cut(s) 137, 908, 1532, 1731, 1779
BfmI CTRYAG 2 cut(s) 1629, 1704
BglI GCCNNNNNGGC 2 cut(s) 92, 1227
Bme1390I CCNGG 3 cut(s) 1518, 1794, 1882
Bme18I GGWCC 4 cut(s) 479, 935, 1469, 1555
BmeT110I CYCGRG 1 cut(s) 1076
BmgBI CACGTC 1 cut(s) 1339
BmgT120I GGNCC 7 cut(s) 479, 935, 1220, 1248, 1313, 1469, 1555
BmrFI CCNGG 3 cut(s) 1518, 1794, 1882
BmsI GCATC 7 cut(s) 73, 348, 644, 754, 963, 1340, 2174
BpiI GAAGAC 2 cut(s) 310, 1975
BpmI CTGGAG 1 cut(s) 1500
Bpu10I CCTNAGC 1 cut(s) 78
Bpu14I TTCGAA 1 cut(s) 859
BpuEI CTTGAG 1 cut(s) 1481
BsaHI GRCGYC 1 cut(s) 792
BsaJI CCNNGG 5 cut(s) 247, 367, 968, 1299, 1881
BsaWI WCCGGW 1 cut(s) 1268
Bsc4I CCNNNNNNNGG 5 cut(s) 128, 495, 571, 1780, 1793
Bse118I RCCGGY 1 cut(s) 1675
Bse1I ACTGG 3 cut(s) 294, 439, 1557
Bse3DI GCAATG 3 cut(s) 267, 628, 828
BseBI CCWGG 3 cut(s) 1518, 1794, 1882
BseDI CCNNGG 5 cut(s) 247, 367, 968, 1299, 1881
BseGI GGATG 5 cut(s) 88, 298, 307, 978, 1243
BseLI CCNNNNNNNGG 5 cut(s) 128, 495, 571, 1780, 1793
BseMI GCAATG 3 cut(s) 267, 628, 828
BseMII CTCAG 3 cut(s) 84, 92, 2113
BseNI ACTGG 3 cut(s) 294, 439, 1557
BseRI GAGGAG 2 cut(s) 717, 1316
BseSI GKGCMC 1 cut(s) 1452
BseXI GCAGC 9 cut(s) 244, 468, 656, 973, 1523, 1547, 1615, 1627, 1666
BseYI CCCAGC 1 cut(s) 647
Bsh1236I CGCG 1 cut(s) 1142
BshFI GGCC 5 cut(s) 445, 1221, 1250, 1314, 1764
BshNI GGYRCC 1 cut(s) 709
BsiHKAI GWGCWC 1 cut(s) 556
BsiHKCI CYCGRG 1 cut(s) 1076
BsiSI CCGG 2 cut(s) 1269, 1676
BslFI GGGAC 3 cut(s) 1065, 1618, 1898
BslI CCNNNNNNNGG 5 cut(s) 128, 495, 571, 1780, 1793
BsmAI GTCTC 3 cut(s) 165, 600, 1104
BsmBI CGTCTC 2 cut(s) 165, 1104
BsmFI GGGAC 3 cut(s) 1065, 1618, 1898
BsmI GAATGC 1 cut(s) 782
BsnI GGCC 5 cut(s) 445, 1221, 1250, 1314, 1764
BsoBI CYCGRG 1 cut(s) 1076
Bsp119I TTCGAA 1 cut(s) 859
Bsp1286I GDGCHC 4 cut(s) 556, 1452, 1786, 2207
Bsp1407I TGTACA 1 cut(s) 732
Bsp143I GATC 6 cut(s) 426, 575, 1188, 1807, 1960, 2009
Bsp19I CCATGG 1 cut(s) 247
BspACI CCGC 3 cut(s) 728, 779, 1736
BspANI GGCC 5 cut(s) 445, 1221, 1250, 1314, 1764
BspCNI CTCAG 3 cut(s) 83, 91, 2112
BspFNI CGCG 1 cut(s) 1142
BspMAI CTGCAG 1 cut(s) 1633
BspPI GGATC 5 cut(s) 570, 583, 1955, 1968, 2017
BspQI GCTCTTC 2 cut(s) 12, 222
BspT104I TTCGAA 1 cut(s) 859
BspT107I GGYRCC 1 cut(s) 709
BsrDI GCAATG 3 cut(s) 267, 628, 828
BsrFI RCCGGY 1 cut(s) 1675
BsrGI TGTACA 1 cut(s) 732
BsrI ACTGG 3 cut(s) 294, 439, 1557
BssAI RCCGGY 1 cut(s) 1675
BssECI CCNNGG 5 cut(s) 247, 367, 968, 1299, 1881
BssMI GATC 6 cut(s) 426, 575, 1188, 1807, 1960, 2009
BssNAI GTATAC 1 cut(s) 1804
BssNI GRCGYC 1 cut(s) 792
BssT1I CCWWGG 2 cut(s) 247, 367
Bst1107I GTATAC 1 cut(s) 1804
Bst2UI CCWGG 3 cut(s) 1518, 1794, 1882
Bst4CI ACNGT 9 cut(s) 385, 558, 566, 755, 806, 952, 969, 1004, 1996
Bst6I CTCTTC 3 cut(s) 12, 222, 1413
BstACI GRCGYC 1 cut(s) 792
BstAPI GCANNNNNTGC 1 cut(s) 725
BstAUI TGTACA 1 cut(s) 732
BstBI TTCGAA 1 cut(s) 859
BstC8I GCNNGC 8 cut(s) 742, 835, 1329, 1618, 1633, 1736, 1740, 1762
BstDEI CTNAG 5 cut(s) 26, 70, 78, 1146, 2099
BstDSI CCRYGG 2 cut(s) 247, 968
BstEII GGTNACC 1 cut(s) 1671
BstF5I GGATG 5 cut(s) 88, 298, 307, 978, 1243
BstFNI CGCG 1 cut(s) 1142
BstHHI GCGC 1 cut(s) 635
BstKTI GATC 6 cut(s) 429, 578, 1191, 1810, 1963, 2012
BstMAI GTCTC 3 cut(s) 165, 600, 1104
BstMBI GATC 6 cut(s) 426, 575, 1188, 1807, 1960, 2009
BstNI CCWGG 3 cut(s) 1518, 1794, 1882
BstNSI RCATGY 1 cut(s) 744
BstPI GGTNACC 1 cut(s) 1671
BstSCI CCNGG 3 cut(s) 1516, 1792, 1880
BstSFI CTRYAG 2 cut(s) 1629, 1704
BstSLI GKGCMC 1 cut(s) 1452
BstUI CGCG 1 cut(s) 1142
BstV1I GCAGC 9 cut(s) 244, 468, 656, 973, 1523, 1547, 1615, 1627, 1666
BstV2I GAAGAC 2 cut(s) 310, 1975
BstX2I RGATCY 2 cut(s) 575, 1960
BstYI RGATCY 2 cut(s) 575, 1960
BstZ17I GTATAC 1 cut(s) 1804
BsuRI GGCC 5 cut(s) 445, 1221, 1250, 1314, 1764
BtgI CCRYGG 2 cut(s) 247, 968
BtgZI GCGATG 1 cut(s) 480
BtrI CACGTC 1 cut(s) 1339
BtsCI GGATG 5 cut(s) 88, 298, 307, 978, 1243
BtsI GCAGTG 2 cut(s) 928, 1237
BtsIMutI CAGTG 3 cut(s) 432, 928, 1237
Cac8I GCNNGC 8 cut(s) 742, 835, 1329, 1618, 1633, 1736, 1740, 1762
CaiI CAGNNNCTG 1 cut(s) 767
CfoI GCGC 1 cut(s) 635
Cfr10I RCCGGY 1 cut(s) 1675
Cfr13I GGNCC 7 cut(s) 479, 935, 1220, 1248, 1313, 1469, 1555
CseI GACGC 2 cut(s) 800, 1121
Csp6I GTAC 4 cut(s) 47, 710, 733, 1521
CviAII CATG 9 cut(s) 59, 155, 209, 248, 357, 620, 741, 1070, 1625
CviQI GTAC 4 cut(s) 47, 710, 733, 1521
DdeI CTNAG 5 cut(s) 26, 70, 78, 1146, 2099
DpnI GATC 6 cut(s) 428, 577, 1190, 1809, 1962, 2011
DpnII GATC 6 cut(s) 426, 575, 1188, 1807, 1960, 2009
EaeI YGGCCR 1 cut(s) 443
Eam1104I CTCTTC 3 cut(s) 12, 222, 1413
EarI CTCTTC 3 cut(s) 12, 222, 1413
Eco130I CCWWGG 2 cut(s) 247, 367
Eco24I GRGCYC 2 cut(s) 1786, 2207
Eco32I GATATC 1 cut(s) 412
Eco47I GGWCC 4 cut(s) 479, 935, 1469, 1555
Eco57I CTGAAG 1 cut(s) 633
Eco88I CYCGRG 1 cut(s) 1076
Eco91I GGTNACC 1 cut(s) 1671
EcoO109I RGGNCCY 1 cut(s) 1220
EcoO65I GGTNACC 1 cut(s) 1671
EcoRII CCWGG 3 cut(s) 1516, 1792, 1880
EcoRV GATATC 1 cut(s) 412
EcoT14I CCWWGG 2 cut(s) 247, 367
EcoT38I GRGCYC 2 cut(s) 1786, 2207
ErhI CCWWGG 2 cut(s) 247, 367
Esp3I CGTCTC 2 cut(s) 165, 1104
FaeI CATG 9 cut(s) 62, 158, 212, 251, 360, 623, 744, 1073, 1628
FalI AAGNNNNNCTT 4 cut(s) 204, 236, 576, 608
FaqI GGGAC 3 cut(s) 1065, 1618, 1898
FatI CATG 9 cut(s) 58, 154, 208, 247, 356, 619, 740, 1069, 1624
FauI CCCGC 1 cut(s) 1743
FauNDI CATATG 3 cut(s) 506, 1444, 1753
FbaI TGATCA 1 cut(s) 1188
FblI GTMKAC 1 cut(s) 1803
FokI GGATG 5 cut(s) 95, 305, 314, 985, 1250
FriOI GRGCYC 2 cut(s) 1786, 2207
FspBI CTAG 5 cut(s) 137, 908, 1532, 1731, 1779
FspI TGCGCA 1 cut(s) 634
GlaI GCGC 1 cut(s) 634
GsaI CCCAGC 1 cut(s) 651
GsuI CTGGAG 1 cut(s) 1500
HaeIII GGCC 5 cut(s) 445, 1221, 1250, 1314, 1764
HapII CCGG 2 cut(s) 1269, 1676
HgaI GACGC 2 cut(s) 800, 1121
HhaI GCGC 1 cut(s) 635
Hin1I GRCGYC 1 cut(s) 792
Hin1II CATG 9 cut(s) 62, 158, 212, 251, 360, 623, 744, 1073, 1628
Hin6I GCGC 1 cut(s) 633
HinP1I GCGC 1 cut(s) 633
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 6 cut(s) 449, 596, 608, 1483, 2055, 2071
HpaII CCGG 2 cut(s) 1269, 1676
HphI GGTGA 6 cut(s) 125, 1006, 1538, 1583, 1665, 2198
Hpy166II GTNNAC 4 cut(s) 181, 733, 1804, 1844
Hpy188I TCNGA 8 cut(s) 551, 613, 763, 939, 1193, 1281, 1343, 1479
Hpy188III TCNNGA 7 cut(s) 80, 377, 573, 1811, 1964, 2052, 2200
Hpy8I GTNNAC 4 cut(s) 181, 733, 1804, 1844
Hpy99I CGWCG 1 cut(s) 1343
HpyAV CCTTC 5 cut(s) 608, 926, 1124, 1785, 1947
HpyCH4III ACNGT 9 cut(s) 385, 558, 566, 755, 806, 952, 969, 1004, 1996
HpyCH4IV ACGT 3 cut(s) 174, 1338, 1647
HpyF3I CTNAG 5 cut(s) 26, 70, 78, 1146, 2099
HpySE526I ACGT 3 cut(s) 174, 1338, 1647
Hsp92I GRCGYC 1 cut(s) 792
Hsp92II CATG 9 cut(s) 62, 158, 212, 251, 360, 623, 744, 1073, 1628
HspAI GCGC 1 cut(s) 633
KpnI GGTACC 1 cut(s) 713
Ksp22I TGATCA 1 cut(s) 1188
Kzo9I GATC 6 cut(s) 426, 575, 1188, 1807, 1960, 2009
LguI GCTCTTC 2 cut(s) 12, 222
LmnI GCTCC 8 cut(s) 551, 1303, 1444, 1519, 1757, 1789, 2050, 2202
Lsp1109I GCAGC 9 cut(s) 244, 468, 656, 973, 1523, 1547, 1615, 1627, 1666
LweI GCATC 7 cut(s) 73, 348, 644, 754, 963, 1340, 2174
MaeI CTAG 5 cut(s) 137, 908, 1532, 1731, 1779
MaeII ACGT 3 cut(s) 174, 1338, 1647
MalI GATC 6 cut(s) 428, 577, 1190, 1809, 1962, 2011
MboI GATC 6 cut(s) 426, 575, 1188, 1807, 1960, 2009
MfeI CAATTG 3 cut(s) 873, 1025, 1395
MflI RGATCY 2 cut(s) 575, 1960
MhlI GDGCHC 4 cut(s) 556, 1452, 1786, 2207
MlsI TGGCCA 1 cut(s) 445
MluNI TGGCCA 1 cut(s) 445
MlyI GAGTC 2 cut(s) 602, 2049
MmeI TCCRAC 2 cut(s) 1321, 1987
Mox20I TGGCCA 1 cut(s) 445
MroXI GAANNNNTTC 1 cut(s) 216
MscI TGGCCA 1 cut(s) 445
MseI TTAA 6 cut(s) 192, 387, 1097, 1175, 1425, 2230
MslI CAYNNNNRTG 1 cut(s) 422
Msp20I TGGCCA 1 cut(s) 445
MspA1I CMGCKG 1 cut(s) 647
MspI CCGG 2 cut(s) 1269, 1676
MspR9I CCNGG 3 cut(s) 1518, 1794, 1882
MunI CAATTG 3 cut(s) 873, 1025, 1395
Mva1269I GAATGC 1 cut(s) 782
MvaI CCWGG 3 cut(s) 1518, 1794, 1882
MvnI CGCG 1 cut(s) 1142
NcoI CCATGG 1 cut(s) 247
NdeI CATATG 3 cut(s) 506, 1444, 1753
NdeII GATC 6 cut(s) 426, 575, 1188, 1807, 1960, 2009
NlaIII CATG 9 cut(s) 62, 158, 212, 251, 360, 623, 744, 1073, 1628
NmeAIII GCCGAG 1 cut(s) 1324
NmuCI GTSAC 4 cut(s) 113, 1671, 1996, 2122
NsbI TGCGCA 1 cut(s) 634
NspI RCATGY 1 cut(s) 744
NspV TTCGAA 1 cut(s) 859
PaeI GCATGC 1 cut(s) 744
PaeR7I CTCGAG 1 cut(s) 1076
PciSI GCTCTTC 2 cut(s) 12, 222
PcsI WCGNNNNNNNCGW 3 cut(s) 426, 562, 792
PctI GAATGC 1 cut(s) 782
PdmI GAANNNNTTC 1 cut(s) 216
PfeI GAWTC 4 cut(s) 449, 596, 1483, 2071
PflFI GACNNNGTC 2 cut(s) 806, 1147
PflMI CCANNNNNTGG 2 cut(s) 495, 1793
PleI GAGTC 2 cut(s) 602, 2049
PpsI GAGTC 2 cut(s) 602, 2049
PshBI ATTAAT 2 cut(s) 192, 1425
Psp6I CCWGG 3 cut(s) 1516, 1792, 1880
PspEI GGTNACC 1 cut(s) 1671
PspFI CCCAGC 1 cut(s) 647
PspGI CCWGG 3 cut(s) 1516, 1792, 1880
PspPI GGNCC 7 cut(s) 479, 935, 1220, 1248, 1313, 1469, 1555
PspXI VCTCGAGB 1 cut(s) 1076
PstI CTGCAG 1 cut(s) 1633
PstNI CAGNNNCTG 1 cut(s) 767
PsuI RGATCY 2 cut(s) 575, 1960
PsyI GACNNNGTC 2 cut(s) 806, 1147
PvuII CAGCTG 1 cut(s) 647
RsaI GTAC 4 cut(s) 48, 711, 734, 1522
RsaNI GTAC 4 cut(s) 47, 710, 733, 1521
RseI CAYNNNNRTG 1 cut(s) 422
SapI GCTCTTC 2 cut(s) 12, 222
SaqAI TTAA 6 cut(s) 192, 387, 1097, 1175, 1425, 2230
Sau3AI GATC 6 cut(s) 426, 575, 1188, 1807, 1960, 2009
Sau96I GGNCC 7 cut(s) 479, 935, 1220, 1248, 1313, 1469, 1555
SchI GAGTC 2 cut(s) 602, 2049
ScrFI CCNGG 3 cut(s) 1518, 1794, 1882
SduI GDGCHC 4 cut(s) 556, 1452, 1786, 2207
SfaNI GCATC 7 cut(s) 73, 348, 644, 754, 963, 1340, 2174
SfcI CTRYAG 2 cut(s) 1629, 1704
Sfr274I CTCGAG 1 cut(s) 1076
SfuI TTCGAA 1 cut(s) 859
SinI GGWCC 4 cut(s) 479, 935, 1469, 1555
SlaI CTCGAG 1 cut(s) 1076
SmiMI CAYNNNNRTG 1 cut(s) 422
SmlI CTYRAG 2 cut(s) 1076, 1496
SmoI CTYRAG 2 cut(s) 1076, 1496
SpeI ACTAGT 1 cut(s) 907
SphI GCATGC 1 cut(s) 744
SsiI CCGC 3 cut(s) 728, 779, 1736
SspI AATATT 1 cut(s) 163
SspMI CTAG 5 cut(s) 137, 908, 1532, 1731, 1779
StyD4I CCNGG 3 cut(s) 1516, 1792, 1880
StyI CCWWGG 2 cut(s) 247, 367
TaaI ACNGT 9 cut(s) 385, 558, 566, 755, 806, 952, 969, 1004, 1996
TaiI ACGT 3 cut(s) 177, 1341, 1650
TaqI TCGA 7 cut(s) 10, 429, 786, 810, 859, 1077, 2138
TatI WGTACW 2 cut(s) 46, 732
TauI GCSGC 1 cut(s) 782
TfiI GAWTC 4 cut(s) 449, 596, 1483, 2071
Tru1I TTAA 6 cut(s) 192, 387, 1097, 1175, 1425, 2230
Tru9I TTAA 6 cut(s) 192, 387, 1097, 1175, 1425, 2230
TscAI CASTG 3 cut(s) 439, 928, 1237
TseFI GTSAC 4 cut(s) 113, 1671, 1996, 2122
TseI GCWGC 9 cut(s) 257, 456, 644, 986, 1511, 1535, 1628, 1640, 1679
Tsp45I GTSAC 4 cut(s) 113, 1671, 1996, 2122
TspDTI ATGAA 5 cut(s) 225, 1000, 1496, 1830, 1926
TspGWI ACGGA 2 cut(s) 879, 1050
TspRI CASTG 3 cut(s) 439, 928, 1237
Tth111I GACNNNGTC 2 cut(s) 806, 1147
Van91I CCANNNNNTGG 2 cut(s) 495, 1793
VpaK11BI GGWCC 4 cut(s) 479, 935, 1469, 1555
VspI ATTAAT 2 cut(s) 192, 1425
XapI RAATTY 6 cut(s) 438, 1085, 1362, 1745, 1967, 2017
XceI RCATGY 1 cut(s) 744
XcmI CCANNNNNNNNNTGG 3 cut(s) 492, 1024, 1565
XhoI CTCGAG 1 cut(s) 1076
XmiI GTMKAC 1 cut(s) 1803
XmnI GAANNNNTTC 1 cut(s) 216
XspI CTAG 5 cut(s) 137, 908, 1532, 1731, 1779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.