MD07G1207200.v1.1

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000390
Physical Location & Seq
Forward (+)
28639516 .. 28640280
765 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1207200.v1.1.491

Sequence Viewer

Length: 540 bp
ATGGCATGCCCACATGCTGCGGGCATGGCTGCATATGTCAAATCATTTCACCCCAATTGGTCACCTGCTGCTATCCTGTCAGCTTTTTCGACTATTGCCGAATTCGCATATGGTGCTGGCCTAATAAATCCTAGTAGGGCTCCGTATCCTGGTTTGGTATATGATGCTACTGAAATCGATTACATAAATTTTTTGTGTGCACATGGCTATAGTACCAAATTATTGAAAGCTCTTACCGGGGATAGTTGCTCATTATCACAATCTAGTCATGGAACACTCAGTGGTCATCTAAACTATCCTTCTGTTGCACTTTCCACCTCGAACCCTAAATCCGTGAATGGCATTTTCAATAGGACCGTCACAAATGTTGGATCACCAAAGTCCACATATAAAGCTAAAGTGAATGCACCACCAGGACTTGAAATCAAAGTTAATCCAAGCGTTCTAAAGTTCACATCTCTCTGGCAGGAGCTATCATTTCAAGTCATGATGAAAGGGTTGATTGAAAAAACCATAGTCTCTGGTTCTCTGGGCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

180

Amino Acids

18.98

Weight (kDa)

9.13

Isoelectric Point (pI)

33.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_6 PF17766 97 - 177 1.1e-19 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 73
Acc36I ACCTGC 1 cut(s) 73
AciI CCGC 1 cut(s) 20
AclWI GGATC 1 cut(s) 379
AcsI RAATTY 2 cut(s) 101, 187
AdeI CACNNNGTG 1 cut(s) 281
AfaI GTAC 1 cut(s) 214
AfiI CCNNNNNNNGG 1 cut(s) 149
AgsI TTSAA 5 cut(s) 226, 349, 422, 482, 506
AjnI CCWGG 2 cut(s) 148, 412
AloI GAACNNNNNNTCC 2 cut(s) 314, 346
AluBI AGCT 4 cut(s) 83, 230, 395, 472
AluI AGCT 4 cut(s) 83, 230, 395, 472
Alw21I GWGCWC 1 cut(s) 202
Alw26I GTCTC 1 cut(s) 523
Alw44I GTGCAC 1 cut(s) 198
AlwI GGATC 1 cut(s) 379
AoxI GGCC 1 cut(s) 118
ApaLI GTGCAC 1 cut(s) 198
ApeKI GCWGC 3 cut(s) 17, 29, 68
ApoI RAATTY 2 cut(s) 101, 187
AspS9I GGNCC 1 cut(s) 354
AsuC2I CCSGG 1 cut(s) 238
AsuHPI GGTGA 3 cut(s) 41, 54, 366
AvaII GGWCC 1 cut(s) 354
BaeGI GKGCMC 2 cut(s) 202, 536
BanII GRGCYC 1 cut(s) 142
Bbv12I GWGCWC 1 cut(s) 202
BbvI GCAGC 3 cut(s) 4, 16, 55
BciT130I CCWGG 2 cut(s) 150, 414
BciVI GTATCC 1 cut(s) 156
BcnI CCSGG 1 cut(s) 238
BcoDI GTCTC 1 cut(s) 523
BfaI CTAG 2 cut(s) 132, 264
BfmI CTRYAG 1 cut(s) 208
BfuAI ACCTGC 1 cut(s) 73
BfuI GTATCC 1 cut(s) 156
BisI GCNGC 3 cut(s) 18, 30, 69
BlsI GCNGC 3 cut(s) 19, 31, 70
Bme1390I CCNGG 3 cut(s) 150, 238, 414
Bme18I GGWCC 1 cut(s) 354
BmgT120I GGNCC 1 cut(s) 354
BmiI GGNNCC 1 cut(s) 141
BmrFI CCNGG 3 cut(s) 150, 238, 414
BmsI GCATC 1 cut(s) 154
BpuMI CCSGG 1 cut(s) 238
Bsa29I ATCGAT 1 cut(s) 177
BsaJI CCNNGG 1 cut(s) 237
Bsc4I CCNNNNNNNGG 1 cut(s) 149
BseBI CCWGG 2 cut(s) 150, 414
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 1 cut(s) 237
BseLI CCNNNNNNNGG 1 cut(s) 149
BseMII CTCAG 1 cut(s) 292
BseSI GKGCMC 2 cut(s) 202, 536
BseXI GCAGC 3 cut(s) 4, 16, 55
BshFI GGCC 1 cut(s) 120
BshVI ATCGAT 1 cut(s) 177
BsiHKAI GWGCWC 1 cut(s) 202
BsiSI CCGG 1 cut(s) 237
BslI CCNNNNNNNGG 1 cut(s) 149
BsmAI GTCTC 1 cut(s) 523
BsmI GAATGC 1 cut(s) 409
BsnI GGCC 1 cut(s) 120
Bsp1286I GDGCHC 3 cut(s) 142, 202, 536
Bsp143I GATC 1 cut(s) 371
BspACI CCGC 1 cut(s) 20
BspANI GGCC 1 cut(s) 120
BspCNI CTCAG 1 cut(s) 291
BspDI ATCGAT 1 cut(s) 177
BspHI TCATGA 1 cut(s) 486
BspLI GGNNCC 1 cut(s) 141
BspMI ACCTGC 1 cut(s) 73
BspPI GGATC 1 cut(s) 379
BssECI CCNNGG 1 cut(s) 237
BssMI GATC 1 cut(s) 371
Bst2UI CCWGG 2 cut(s) 150, 414
Bst4CI ACNGT 1 cut(s) 358
BstAPI GCANNNNNTGC 1 cut(s) 113
BstC8I GCNNGC 3 cut(s) 7, 22, 118
BstDEI CTNAG 1 cut(s) 278
BstEII GGTNACC 1 cut(s) 60
BstKTI GATC 1 cut(s) 374
BstMAI GTCTC 1 cut(s) 523
BstMBI GATC 1 cut(s) 371
BstMWI GCNNNNNNNGC 3 cut(s) 26, 104, 113
BstNI CCWGG 2 cut(s) 150, 414
BstNSI RCATGY 2 cut(s) 9, 17
BstPI GGTNACC 1 cut(s) 60
BstSCI CCNGG 3 cut(s) 148, 236, 412
BstSFI CTRYAG 1 cut(s) 208
BstSLI GKGCMC 2 cut(s) 202, 536
BstV1I GCAGC 3 cut(s) 4, 16, 55
Bsu15I ATCGAT 1 cut(s) 177
BsuI GTATCC 1 cut(s) 156
BsuRI GGCC 1 cut(s) 120
BsuTUI ATCGAT 1 cut(s) 177
BtsIMutI CAGTG 1 cut(s) 286
BveI ACCTGC 1 cut(s) 73
Cac8I GCNNGC 3 cut(s) 7, 22, 118
CciI TCATGA 1 cut(s) 486
Cfr13I GGNCC 1 cut(s) 354
ClaI ATCGAT 1 cut(s) 177
Csp6I GTAC 1 cut(s) 213
CviAII CATG 6 cut(s) 6, 14, 25, 203, 269, 487
CviJI RGCY 8 cut(s) 29, 83, 120, 140, 207, 230, 395, 472
CviKI_1 RGCY 8 cut(s) 29, 83, 120, 140, 207, 230, 395, 472
CviQI GTAC 1 cut(s) 213
DdeI CTNAG 1 cut(s) 278
DpnI GATC 1 cut(s) 373
DpnII GATC 1 cut(s) 371
DraIII CACNNNGTG 1 cut(s) 281
Eco24I GRGCYC 1 cut(s) 142
Eco47I GGWCC 1 cut(s) 354
Eco91I GGTNACC 1 cut(s) 60
EcoO65I GGTNACC 1 cut(s) 60
EcoRI GAATTC 1 cut(s) 101
EcoRII CCWGG 2 cut(s) 148, 412
EcoT38I GRGCYC 1 cut(s) 142
FaeI CATG 6 cut(s) 9, 17, 28, 206, 272, 490
FatI CATG 6 cut(s) 5, 13, 24, 202, 268, 486
FauI CCCGC 1 cut(s) 13
FauNDI CATATG 2 cut(s) 34, 109
Fnu4HI GCNGC 3 cut(s) 18, 30, 69
FriOI GRGCYC 1 cut(s) 142
Fsp4HI GCNGC 3 cut(s) 18, 30, 69
FspBI CTAG 2 cut(s) 132, 264
GluI GCNGC 3 cut(s) 18, 30, 69
HaeIII GGCC 1 cut(s) 120
HapII CCGG 1 cut(s) 237
Hin1II CATG 6 cut(s) 9, 17, 28, 206, 272, 490
HpaII CCGG 1 cut(s) 237
HphI GGTGA 3 cut(s) 41, 54, 366
Hpy166II GTNNAC 3 cut(s) 200, 384, 453
Hpy188III TCNNGA 1 cut(s) 487
Hpy8I GTNNAC 3 cut(s) 200, 384, 453
HpyAV CCTTC 1 cut(s) 309
HpyCH4III ACNGT 1 cut(s) 358
HpyCH4V TGCA 4 cut(s) 32, 200, 308, 407
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 104, 113
HpyF3I CTNAG 1 cut(s) 278
Hsp92II CATG 6 cut(s) 9, 17, 28, 206, 272, 490
Kzo9I GATC 1 cut(s) 371
LmnI GCTCC 2 cut(s) 145, 469
Lsp1109I GCAGC 3 cut(s) 4, 16, 55
LweI GCATC 1 cut(s) 154
MaeI CTAG 2 cut(s) 132, 264
MaeIII GTNAC 2 cut(s) 60, 358
MalI GATC 1 cut(s) 373
MboI GATC 1 cut(s) 371
MfeI CAATTG 1 cut(s) 55
MhlI GDGCHC 3 cut(s) 142, 202, 536
MluCI AATT 4 cut(s) 55, 101, 187, 218
MmeI TCCRAC 1 cut(s) 349
MnlI CCTC 1 cut(s) 328
MseI TTAA 1 cut(s) 432
MspI CCGG 1 cut(s) 237
MspR9I CCNGG 3 cut(s) 150, 238, 414
MunI CAATTG 1 cut(s) 55
Mva1269I GAATGC 1 cut(s) 409
MvaI CCWGG 2 cut(s) 150, 414
MwoI GCNNNNNNNGC 3 cut(s) 26, 104, 113
NciI CCSGG 1 cut(s) 238
NdeI CATATG 2 cut(s) 34, 109
NdeII GATC 1 cut(s) 371
NlaIII CATG 6 cut(s) 9, 17, 28, 206, 272, 490
NlaIV GGNNCC 1 cut(s) 141
NmuCI GTSAC 2 cut(s) 60, 358
NspI RCATGY 2 cut(s) 9, 17
PaeI GCATGC 1 cut(s) 9
PagI TCATGA 1 cut(s) 486
PaqCI CACCTGC 1 cut(s) 73
PctI GAATGC 1 cut(s) 409
PkrI GCNGC 3 cut(s) 19, 31, 70
Psp6I CCWGG 2 cut(s) 148, 412
PspEI GGTNACC 1 cut(s) 60
PspGI CCWGG 2 cut(s) 148, 412
PspN4I GGNNCC 1 cut(s) 141
PspPI GGNCC 1 cut(s) 354
RsaI GTAC 1 cut(s) 214
RsaNI GTAC 1 cut(s) 213
SaqAI TTAA 1 cut(s) 432
SatI GCNGC 3 cut(s) 18, 30, 69
Sau3AI GATC 1 cut(s) 371
Sau96I GGNCC 1 cut(s) 354
ScrFI CCNGG 3 cut(s) 150, 238, 414
SduI GDGCHC 3 cut(s) 142, 202, 536
SetI ASST 6 cut(s) 67, 85, 232, 320, 397, 474
SfaNI GCATC 1 cut(s) 154
SfcI CTRYAG 1 cut(s) 208
SinI GGWCC 1 cut(s) 354
SphI GCATGC 1 cut(s) 9
Sse9I AATT 4 cut(s) 55, 101, 187, 218
SsiI CCGC 1 cut(s) 20
SspMI CTAG 2 cut(s) 132, 264
StyD4I CCNGG 3 cut(s) 148, 236, 412
TaaI ACNGT 1 cut(s) 358
TaqI TCGA 3 cut(s) 89, 177, 320
TasI AATT 4 cut(s) 55, 101, 187, 218
Tru1I TTAA 1 cut(s) 432
Tru9I TTAA 1 cut(s) 432
TscAI CASTG 1 cut(s) 286
TseFI GTSAC 2 cut(s) 60, 358
TseI GCWGC 3 cut(s) 17, 29, 68
Tsp45I GTSAC 2 cut(s) 60, 358
TspDTI ATGAA 1 cut(s) 506
TspGWI ACGGA 2 cut(s) 132, 322
TspRI CASTG 1 cut(s) 286
VneI GTGCAC 1 cut(s) 198
VpaK11BI GGWCC 1 cut(s) 354
XapI RAATTY 2 cut(s) 101, 187
XceI RCATGY 2 cut(s) 9, 17
XspI CTAG 2 cut(s) 132, 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.