RchiOBHm_Chr1g0325901

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000390
Physical Location & Seq
Reverse (-)
14017730 .. 14020477
2748 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55558

Sequence Viewer

Length: 1932 bp
ATGATGTATACAGGAATGGATGGTGTAGTGTCTGTCTTCCCAAGCAAACAAAGGAGCGTCAAAACAACAAAGTCATGGAACTTCCTTGAGTTTCCAGAAACAGTTAAGCGAAGCGCCCTTGAAAGGAATATCATTGTCGGTGTGATGGACACTGGAATTTGGCCAGAGTCGGACAGCTTTAGTGATGCTGGTTTTGGTCCTCCACCTGAGAAATGGAAAGGCAAATGTCAAGGCAATGGAAATCTTACTTGTAACAATAAAATCATCGGAGCACAGTATTACCGTGCTTCTCGATCCTTCGAAAAAGATGTTGAGTCTCCAAGAGACTTAAATGGTCATGGGACGCATACTGCATCAACTGCAGCTGGGAACACAGTGAGCAAGGCAAGCCTAAATGGTTTAGGATTGGGAACAGCAAGAGGTGGGGTGCCATCAGCACGTATTGCCATGTACAAAGTATGCTGGAGCAGCAGCCCTGCTTGTGAAGATGTTGACATTTTAGCGGCATTCGATGATGCCATTGCGGACGGTGTCGACATTCTCTCTATTTCTATCGGGGGCCCTATTCCAGTTGATTATTTGACTGATTCACTTGCAATTGGGTCATTTCACGCTACCAGAAATGGGATATTAGTTTCCATGGCCGCTGGTAACGAAGGTCCGGGAGCAAAAACTGTGACAAACTTTGCACCATGGCAACTTTCTGTAGCTGCTACCACCATAAACCGCCAATTCATCACCAAGGTTCAATTGGGTAACGGTAAAATCTATGAGGGACTTTTACCAAACATATATGATCTCCGGGGTAAATTCTATCCTTTAATTTATGGTGGAGACGCACCCAACAAAAAAGTAGGTGCTAAAGGGACCGTCTCCAGGTTTTGTATCATAGATACCTTAGAAGAAGATTTGATCAAAGATAAAATTGTGCTTTGTGATGGCCCTGTTGGGGGTGGAGCCATATTGGGTGGTGCAGCCGGAATTGTTCTAACAGGACAAAAAGTCGCTGATGAATTGCTTGAACCTCTTCCCGTGCCTGCATCAAACGTCGGATTGGAAGAAAATAGCAACATTTACAAATACATAAACTTAACAAGGAACGCAACTGCAACTATTTGGAAAAGTGAAGAGATTAATGATGTATTGGCTCCATACGTGCCCTCATACTCATCAAGGGGTCCAAATCCGATCAATCCCAACATTCTCAAGCCAGATTTAGCGGCTCCAGGAACTTACATTCTAGCAGCATATCCCCCAAGTGGTGGTGGCAGTAGAGCTGGATATTATTTAGAAACTGGGACATCAATGGCGTGCCCACATGCTACAGCCGTAGCTGCATACGTCAAATCATTTCACCCTAAATGGTCACCGGCTGCTATCCAATCGGCTCTCATCACTACCGCTAAATCCATGAGTGCCAAAACTAGCCCTGATGCTGAATTTGCATACGGAGCTGGCCTAATAAACCCTTCTAAGGCTCCATATCCTGGTTTGGTGTACGATCTTGACGAACAAGACTACATAGATTTTTTGTGTTCACAAGGATATAGTGGCAAACTATTGCAAGCCATAACCAGGGACAACACTAGTTGTTCATCAAAATCTAATAATGGAACAACCAATGATCTGAACTATCCTTCTTTTGCACTTTCCATCAAGGATCCTAAATTTGCCAATGGGGTCTTCCATAGGACTGTTACTAATGTTGGATCGTCGAATTCCACATATAGAGTTAAAGTGGTGGCTCCTTTAGGACTCAAAATCAATGTGAATCCAAGTGTGATATCATTCACATCTCTCGGGCAAAAGAAATCTTTTGTTGTCACTATAAACGGGCCGATTGAGAAATCAAATTTAGTCTCTGCATCTTTGGTGTGGGATGATGGTGTTTTCCAAGTCAGGAGCCCCATCGTTGTCTATGTTGCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

643

Amino Acids

68.24

Weight (kDa)

7.91

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 42 - 486 6.5e-45 Subtilase family
fn3_6 PF17766 542 - 639 1.9e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 427
AccB7I CCANNNNNTGG 2 cut(s) 1262, 1487
AccI GTMKAC 2 cut(s) 8, 534
AciI CCGC 6 cut(s) 503, 524, 645, 727, 1220, 1401
AclWI GGATC 4 cut(s) 288, 1655, 1668, 1717
AcoI YGGCCR 2 cut(s) 161, 642
AcsI RAATTY 6 cut(s) 156, 809, 1439, 1667, 1717, 1852
AfaI GTAC 2 cut(s) 452, 1499
AgsI TTSAA 3 cut(s) 122, 749, 1022
AhdI GACNNNNNGTC 1 cut(s) 1001
AhlI ACTAGT 1 cut(s) 1586
AjnI CCWGG 4 cut(s) 875, 1225, 1486, 1574
AjuI GAANNNNNNNTTGG 2 cut(s) 1667, 1699
AluBI AGCT 6 cut(s) 177, 365, 710, 1277, 1334, 1454
AluI AGCT 6 cut(s) 177, 365, 710, 1277, 1334, 1454
Alw21I GWGCWC 1 cut(s) 274
Alw26I GTCTC 5 cut(s) 318, 321, 828, 877, 1864
AlwI GGATC 4 cut(s) 288, 1655, 1668, 1717
Ama87I CYCGRG 1 cut(s) 1799
AoxI GGCC 6 cut(s) 161, 559, 642, 940, 1456, 1835
ApaI GGGCCC 1 cut(s) 563
ApeKI GCWGC 8 cut(s) 362, 468, 471, 710, 974, 1244, 1334, 1373
ApoI RAATTY 6 cut(s) 156, 809, 1439, 1667, 1717, 1852
AseI ATTAAT 1 cut(s) 1134
Asp700I GAANNNNTTC 1 cut(s) 1026
AspLEI GCGC 1 cut(s) 116
AspS9I GGNCC 8 cut(s) 197, 559, 560, 659, 867, 941, 1178, 1835
AsuC2I CCSGG 2 cut(s) 663, 803
AsuHPI GGTGA 3 cut(s) 730, 1346, 1359
AsuII TTCGAA 1 cut(s) 300
AvaI CYCGRG 1 cut(s) 1799
AvaII GGWCC 4 cut(s) 197, 659, 867, 1178
BaeGI GKGCMC 3 cut(s) 563, 1161, 1316
BalI TGGCCA 1 cut(s) 163
BamHI GGATCC 1 cut(s) 1660
BanI GGYRCC 1 cut(s) 427
BanII GRGCYC 2 cut(s) 563, 1907
BbsI GAAGAC 2 cut(s) 28, 1675
Bbv12I GWGCWC 1 cut(s) 274
BbvI GCAGC 8 cut(s) 374, 480, 483, 697, 986, 1256, 1321, 1360
BccI CCATC 7 cut(s) 14, 139, 439, 932, 1661, 1877, 1916
BceAI ACGGC 1 cut(s) 1313
BciT130I CCWGG 4 cut(s) 877, 1227, 1488, 1576
BclI TGATCA 1 cut(s) 912
BcnI CCSGG 2 cut(s) 663, 803
BcoDI GTCTC 5 cut(s) 318, 321, 828, 877, 1864
BcuI ACTAGT 1 cut(s) 1586
BfaI CTAG 3 cut(s) 1241, 1425, 1587
BfmI CTRYAG 3 cut(s) 360, 705, 1323
BfoI RGCGCY 1 cut(s) 117
Bme1390I CCNGG 6 cut(s) 663, 803, 877, 1227, 1488, 1576
Bme18I GGWCC 4 cut(s) 197, 659, 867, 1178
BmeRI GACNNNNNGTC 1 cut(s) 1001
BmeT110I CYCGRG 1 cut(s) 1799
BmgT120I GGNCC 8 cut(s) 197, 559, 560, 659, 867, 941, 1178, 1835
BmrFI CCNGG 6 cut(s) 663, 803, 877, 1227, 1488, 1576
BmrI ACTGGG 1 cut(s) 1305
BmsI GCATC 6 cut(s) 175, 362, 505, 1049, 1423, 1874
BmuI ACTGGG 1 cut(s) 1305
BpiI GAAGAC 2 cut(s) 28, 1675
BpmI CTGGAG 3 cut(s) 484, 859, 1209
Bpu14I TTCGAA 1 cut(s) 300
BpuEI CTTGAG 2 cut(s) 107, 1190
BpuMI CCSGG 2 cut(s) 663, 803
BsaAI YACGTR 2 cut(s) 440, 1156
BsaJI CCNNGG 5 cut(s) 639, 692, 741, 802, 1575
Bse118I RCCGGY 1 cut(s) 1369
Bse1I ACTGG 3 cut(s) 157, 569, 1300
Bse3DI GCAATG 2 cut(s) 241, 519
BseBI CCWGG 4 cut(s) 877, 1227, 1488, 1576
BseDI CCNNGG 5 cut(s) 639, 692, 741, 802, 1575
BseGI GGATG 2 cut(s) 25, 1885
BseMI GCAATG 2 cut(s) 241, 519
BseMII CTCAG 1 cut(s) 198
BseNI ACTGG 3 cut(s) 157, 569, 1300
BseSI GKGCMC 3 cut(s) 563, 1161, 1316
BseXI GCAGC 8 cut(s) 374, 480, 483, 697, 986, 1256, 1321, 1360
BseYI CCCAGC 1 cut(s) 365
BsgI GTGCAG 1 cut(s) 993
BshFI GGCC 6 cut(s) 163, 561, 644, 942, 1458, 1837
BshNI GGYRCC 1 cut(s) 427
BsiHKAI GWGCWC 1 cut(s) 274
BsiHKCI CYCGRG 1 cut(s) 1799
BsiSI CCGG 4 cut(s) 662, 802, 978, 1370
BslFI GGGAC 5 cut(s) 355, 789, 880, 1312, 1592
BsmAI GTCTC 5 cut(s) 318, 321, 828, 877, 1864
BsmBI CGTCTC 2 cut(s) 828, 877
BsmFI GGGAC 5 cut(s) 355, 789, 880, 1312, 1592
BsmI GAATGC 1 cut(s) 506
BsnI GGCC 6 cut(s) 163, 561, 644, 942, 1458, 1837
BsoBI CYCGRG 1 cut(s) 1799
Bsp119I TTCGAA 1 cut(s) 300
Bsp120I GGGCCC 1 cut(s) 559
Bsp1286I GDGCHC 5 cut(s) 274, 563, 1161, 1316, 1907
Bsp1407I TGTACA 1 cut(s) 450
Bsp143I GATC 8 cut(s) 293, 796, 912, 1188, 1501, 1624, 1660, 1709
Bsp19I CCATGG 2 cut(s) 639, 692
BspACI CCGC 6 cut(s) 503, 524, 645, 727, 1220, 1401
BspANI GGCC 6 cut(s) 163, 561, 644, 942, 1458, 1837
BspCNI CTCAG 1 cut(s) 199
BspMAI CTGCAG 1 cut(s) 364
BspPI GGATC 4 cut(s) 288, 1655, 1668, 1717
BspT104I TTCGAA 1 cut(s) 300
BspT107I GGYRCC 1 cut(s) 427
BsrDI GCAATG 2 cut(s) 241, 519
BsrFI RCCGGY 1 cut(s) 1369
BsrGI TGTACA 1 cut(s) 450
BsrI ACTGG 3 cut(s) 157, 569, 1300
BssAI RCCGGY 1 cut(s) 1369
BssECI CCNNGG 5 cut(s) 639, 692, 741, 802, 1575
BssMI GATC 8 cut(s) 293, 796, 912, 1188, 1501, 1624, 1660, 1709
BssNAI GTATAC 1 cut(s) 9
BssT1I CCWWGG 3 cut(s) 639, 692, 741
Bst1107I GTATAC 1 cut(s) 9
Bst2UI CCWGG 4 cut(s) 877, 1227, 1488, 1576
Bst4CI ACNGT 9 cut(s) 103, 276, 284, 376, 530, 676, 761, 871, 1696
Bst6I CTCTTC 2 cut(s) 1032, 1122
BstAPI GCANNNNNTGC 2 cut(s) 359, 443
BstAUI TGTACA 1 cut(s) 450
BstBAI YACGTR 2 cut(s) 440, 1156
BstBI TTCGAA 1 cut(s) 300
BstC8I GCNNGC 5 cut(s) 388, 1038, 1312, 1456, 1566
BstDEI CTNAG 3 cut(s) 207, 898, 1473
BstDSI CCRYGG 2 cut(s) 639, 692
BstEII GGTNACC 1 cut(s) 1365
BstF5I GGATG 2 cut(s) 25, 1885
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 8 cut(s) 296, 799, 915, 1191, 1504, 1627, 1663, 1712
BstMAI GTCTC 5 cut(s) 318, 321, 828, 877, 1864
BstMBI GATC 8 cut(s) 293, 796, 912, 1188, 1501, 1624, 1660, 1709
BstMWI GCNNNNNNNGC 7 cut(s) 359, 387, 443, 468, 1334, 1442, 1451
BstNI CCWGG 4 cut(s) 877, 1227, 1488, 1576
BstNSI RCATGY 1 cut(s) 1322
BstPI GGTNACC 1 cut(s) 1365
BstSCI CCNGG 6 cut(s) 661, 801, 875, 1225, 1486, 1574
BstSFI CTRYAG 3 cut(s) 360, 705, 1323
BstSLI GKGCMC 3 cut(s) 563, 1161, 1316
BstV1I GCAGC 8 cut(s) 374, 480, 483, 697, 986, 1256, 1321, 1360
BstV2I GAAGAC 2 cut(s) 28, 1675
BstX2I RGATCY 1 cut(s) 1660
BstYI RGATCY 1 cut(s) 1660
BstZ17I GTATAC 1 cut(s) 9
BsuRI GGCC 6 cut(s) 163, 561, 644, 942, 1458, 1837
BtgI CCRYGG 2 cut(s) 639, 692
BtsCI GGATG 2 cut(s) 25, 1885
BtsIMutI CAGTG 2 cut(s) 150, 381
Cac8I GCNNGC 5 cut(s) 388, 1038, 1312, 1456, 1566
CfoI GCGC 1 cut(s) 116
Cfr10I RCCGGY 1 cut(s) 1369
Cfr13I GGNCC 8 cut(s) 197, 559, 560, 659, 867, 941, 1178, 1835
CseI GACGC 3 cut(s) 46, 352, 845
Csp6I GTAC 2 cut(s) 451, 1498
CviAII CATG 7 cut(s) 75, 338, 448, 640, 693, 1319, 1411
CviQI GTAC 2 cut(s) 451, 1498
DdeI CTNAG 3 cut(s) 207, 898, 1473
DpnI GATC 8 cut(s) 295, 798, 914, 1190, 1503, 1626, 1662, 1711
DpnII GATC 8 cut(s) 293, 796, 912, 1188, 1501, 1624, 1660, 1709
DriI GACNNNNNGTC 1 cut(s) 1001
EaeI YGGCCR 2 cut(s) 161, 642
Eam1104I CTCTTC 2 cut(s) 1032, 1122
Eam1105I GACNNNNNGTC 1 cut(s) 1001
EarI CTCTTC 2 cut(s) 1032, 1122
Eco130I CCWWGG 3 cut(s) 639, 692, 741
Eco24I GRGCYC 2 cut(s) 563, 1907
Eco32I GATATC 1 cut(s) 1785
Eco47I GGWCC 4 cut(s) 197, 659, 867, 1178
Eco88I CYCGRG 1 cut(s) 1799
Eco91I GGTNACC 1 cut(s) 1365
EcoO109I RGGNCCY 2 cut(s) 559, 560
EcoO65I GGTNACC 1 cut(s) 1365
EcoRI GAATTC 1 cut(s) 1717
EcoRII CCWGG 4 cut(s) 875, 1225, 1486, 1574
EcoRV GATATC 1 cut(s) 1785
EcoT14I CCWWGG 3 cut(s) 639, 692, 741
EcoT38I GRGCYC 2 cut(s) 563, 1907
ErhI CCWWGG 3 cut(s) 639, 692, 741
Esp3I CGTCTC 2 cut(s) 828, 877
FaeI CATG 7 cut(s) 78, 341, 451, 643, 696, 1322, 1414
FaqI GGGAC 5 cut(s) 355, 789, 880, 1312, 1592
FatI CATG 7 cut(s) 74, 337, 447, 639, 692, 1318, 1410
FbaI TGATCA 1 cut(s) 912
FblI GTMKAC 2 cut(s) 8, 534
FokI GGATG 2 cut(s) 32, 1892
FriOI GRGCYC 2 cut(s) 563, 1907
FspBI CTAG 3 cut(s) 1241, 1425, 1587
GlaI GCGC 1 cut(s) 115
GsaI CCCAGC 1 cut(s) 369
GsuI CTGGAG 3 cut(s) 484, 859, 1209
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 6 cut(s) 163, 561, 644, 942, 1458, 1837
HapII CCGG 4 cut(s) 662, 802, 978, 1370
HgaI GACGC 3 cut(s) 46, 352, 845
HhaI GCGC 1 cut(s) 116
Hin1II CATG 7 cut(s) 78, 341, 451, 643, 696, 1322, 1414
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HincII GTYRAC 2 cut(s) 493, 535
HindII GTYRAC 2 cut(s) 493, 535
HinfI GANTC 5 cut(s) 167, 314, 587, 1755, 1771
HpaII CCGG 4 cut(s) 662, 802, 978, 1370
HphI GGTGA 3 cut(s) 730, 1346, 1359
Hpy166II GTNNAC 5 cut(s) 9, 493, 535, 1498, 1538
Hpy188I TCNGA 5 cut(s) 172, 269, 1052, 1188, 1629
Hpy188III TCNNGA 4 cut(s) 95, 291, 1505, 1900
Hpy8I GTNNAC 5 cut(s) 9, 493, 535, 1498, 1538
Hpy99I CGWCG 2 cut(s) 1052, 1717
HpyAV CCTTC 4 cut(s) 307, 650, 1479, 1647
HpyCH4III ACNGT 9 cut(s) 103, 276, 284, 376, 530, 676, 761, 871, 1696
HpyCH4IV ACGT 4 cut(s) 439, 1047, 1155, 1341
HpyF10VI GCNNNNNNNGC 7 cut(s) 359, 387, 443, 468, 1334, 1442, 1451
HpyF3I CTNAG 3 cut(s) 207, 898, 1473
HpySE526I ACGT 4 cut(s) 439, 1047, 1155, 1341
Hsp92II CATG 7 cut(s) 78, 341, 451, 643, 696, 1322, 1414
HspAI GCGC 1 cut(s) 114
Ksp22I TGATCA 1 cut(s) 912
Kzo9I GATC 8 cut(s) 293, 796, 912, 1188, 1501, 1624, 1660, 1709
Lsp1109I GCAGC 8 cut(s) 374, 480, 483, 697, 986, 1256, 1321, 1360
LweI GCATC 6 cut(s) 175, 362, 505, 1049, 1423, 1874
MaeI CTAG 3 cut(s) 1241, 1425, 1587
MaeII ACGT 4 cut(s) 439, 1047, 1155, 1341
MaeIII GTNAC 7 cut(s) 251, 650, 676, 755, 1365, 1696, 1822
MalI GATC 8 cut(s) 295, 798, 914, 1190, 1503, 1626, 1662, 1711
MboI GATC 8 cut(s) 293, 796, 912, 1188, 1501, 1624, 1660, 1709
MboII GAAGA 8 cut(s) 28, 497, 914, 917, 1019, 1070, 1139, 1675
MfeI CAATTG 2 cut(s) 597, 749
MflI RGATCY 1 cut(s) 1660
MhlI GDGCHC 5 cut(s) 274, 563, 1161, 1316, 1907
MlsI TGGCCA 1 cut(s) 163
MluNI TGGCCA 1 cut(s) 163
MlyI GAGTC 3 cut(s) 176, 323, 1749
MmeI TCCRAC 3 cut(s) 150, 1030, 1687
MnlI CCTC 5 cut(s) 210, 413, 766, 1035, 1171
Mox20I TGGCCA 1 cut(s) 163
MroXI GAANNNNTTC 1 cut(s) 1026
MscI TGGCCA 1 cut(s) 163
MseI TTAA 7 cut(s) 105, 329, 821, 1091, 1134, 1734, 1930
Msp20I TGGCCA 1 cut(s) 163
MspA1I CMGCKG 2 cut(s) 365, 647
MspI CCGG 4 cut(s) 662, 802, 978, 1370
MspR9I CCNGG 6 cut(s) 663, 803, 877, 1227, 1488, 1576
MunI CAATTG 2 cut(s) 597, 749
Mva1269I GAATGC 1 cut(s) 506
MvaI CCWGG 4 cut(s) 877, 1227, 1488, 1576
MwoI GCNNNNNNNGC 7 cut(s) 359, 387, 443, 468, 1334, 1442, 1451
NciI CCSGG 2 cut(s) 663, 803
NcoI CCATGG 2 cut(s) 639, 692
NdeII GATC 8 cut(s) 293, 796, 912, 1188, 1501, 1624, 1660, 1709
NlaIII CATG 7 cut(s) 78, 341, 451, 643, 696, 1322, 1414
NmuCI GTSAC 3 cut(s) 676, 1365, 1822
NspI RCATGY 1 cut(s) 1322
NspV TTCGAA 1 cut(s) 300
PcsI WCGNNNNNNNCGW 1 cut(s) 1506
PctI GAATGC 1 cut(s) 506
PdmI GAANNNNTTC 1 cut(s) 1026
PfeI GAWTC 2 cut(s) 587, 1771
PflFI GACNNNGTC 1 cut(s) 530
PflMI CCANNNNNTGG 2 cut(s) 1262, 1487
PfoI TCCNGGA 2 cut(s) 661, 1225
PleI GAGTC 3 cut(s) 175, 322, 1749
PpsI GAGTC 3 cut(s) 175, 322, 1749
Ppu21I YACGTR 2 cut(s) 440, 1156
PshBI ATTAAT 1 cut(s) 1134
Psp6I CCWGG 4 cut(s) 875, 1225, 1486, 1574
PspEI GGTNACC 1 cut(s) 1365
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 4 cut(s) 875, 1225, 1486, 1574
PspOMI GGGCCC 1 cut(s) 559
PspPI GGNCC 8 cut(s) 197, 559, 560, 659, 867, 941, 1178, 1835
PstI CTGCAG 1 cut(s) 364
PsuI RGATCY 1 cut(s) 1660
PsyI GACNNNGTC 1 cut(s) 530
PvuII CAGCTG 1 cut(s) 365
RsaI GTAC 2 cut(s) 452, 1499
RsaNI GTAC 2 cut(s) 451, 1498
SalI GTCGAC 1 cut(s) 533
SaqAI TTAA 7 cut(s) 105, 329, 821, 1091, 1134, 1734, 1930
Sau3AI GATC 8 cut(s) 293, 796, 912, 1188, 1501, 1624, 1660, 1709
Sau96I GGNCC 8 cut(s) 197, 559, 560, 659, 867, 941, 1178, 1835
SchI GAGTC 3 cut(s) 176, 323, 1749
ScrFI CCNGG 6 cut(s) 663, 803, 877, 1227, 1488, 1576
SduI GDGCHC 5 cut(s) 274, 563, 1161, 1316, 1907
SfaNI GCATC 6 cut(s) 175, 362, 505, 1049, 1423, 1874
SfcI CTRYAG 3 cut(s) 360, 705, 1323
SfuI TTCGAA 1 cut(s) 300
SinI GGWCC 4 cut(s) 197, 659, 867, 1178
SmlI CTYRAG 2 cut(s) 86, 1205
SmoI CTYRAG 2 cut(s) 86, 1205
SpeI ACTAGT 1 cut(s) 1586
SsiI CCGC 6 cut(s) 503, 524, 645, 727, 1220, 1401
SspMI CTAG 3 cut(s) 1241, 1425, 1587
StyD4I CCNGG 6 cut(s) 661, 801, 875, 1225, 1486, 1574
StyI CCWWGG 3 cut(s) 639, 692, 741
TaaI ACNGT 9 cut(s) 103, 276, 284, 376, 530, 676, 761, 871, 1696
TaiI ACGT 4 cut(s) 442, 1050, 1158, 1344
TaqI TCGA 5 cut(s) 292, 300, 510, 534, 1715
TatI WGTACW 1 cut(s) 450
TauI GCSGC 3 cut(s) 506, 647, 1223
TfiI GAWTC 2 cut(s) 587, 1771
Tru1I TTAA 7 cut(s) 105, 329, 821, 1091, 1134, 1734, 1930
Tru9I TTAA 7 cut(s) 105, 329, 821, 1091, 1134, 1734, 1930
TscAI CASTG 2 cut(s) 157, 381
TseFI GTSAC 3 cut(s) 676, 1365, 1822
TseI GCWGC 8 cut(s) 362, 468, 471, 710, 974, 1244, 1334, 1373
Tsp45I GTSAC 3 cut(s) 676, 1365, 1822
TspDTI ATGAA 3 cut(s) 724, 1026, 1584
TspGWI ACGGA 1 cut(s) 1464
TspRI CASTG 2 cut(s) 157, 381
Tth111I GACNNNGTC 1 cut(s) 530
Van91I CCANNNNNTGG 2 cut(s) 1262, 1487
VpaK11BI GGWCC 4 cut(s) 197, 659, 867, 1178
VspI ATTAAT 1 cut(s) 1134
XapI RAATTY 6 cut(s) 156, 809, 1439, 1667, 1717, 1852
XceI RCATGY 1 cut(s) 1322
XcmI CCANNNNNNNNNTGG 2 cut(s) 210, 748
XmiI GTMKAC 2 cut(s) 8, 534
XmnI GAANNNNTTC 1 cut(s) 1026
XspI CTAG 3 cut(s) 1241, 1425, 1587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.