Prupe.2G242200_v2.0.a1

Cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000390
Physical Location & Seq
Forward (+)
26129474 .. 26133359
3886 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G242200.1

Sequence Viewer

Length: 2325 bp
ATGAGCTTGCCCCATCTTTTCCAAGTAGTACGTTTTCTAGGTTTTGTAGTGAGACCCCCCATAATGGATTTAGTTCCTTGGTCGCGAAAAGGGTTTTTCCATGTAGTGTGGTTTTTCCTTCTCGGCCTCACATGTACTCTGCTAGTAGATGTCACTCATTCTGATGCTCATCCGGATACCAGAAAGGTTTATATTGTGTACATGGGGGACAAGCCAAAGCTCGACATTCCCACCACTACCACCTTGCCTCTTCATGTAGACATGCTTCAAAACGTAGTTGGCAGCAGCAATATTGCACAAGAACCTCTCCTACTGCACAGCTACAAGAGAAGCTTCAATGGCTTTGCTGCAAAGCTAACAGAGGAAGAAGCTCAAAAAATGGCTGGAATGGCTGGTGTGGTGTCTGTTTTCCCTAATGAGAAGCAGAAGCTCCACACAACAAGGTCATGGAACTTCATCGGGTTTCATGAAAACGTGAAGAGAAGCACTGTTGAAAGCGATATCATCGTTGGGGTGATCGACACTGGAGTTTGGCCGGAATCTGCCAGCTTCAGTGATGCCGGGTTTGGTCCACCACCAAAAAAATGGAAAGGCACATGCCAAGGCTCATCCAATTTTACTTGCAACAATAAAATCATTGGAGCACGGTATTACCGCATTAGTGAACCCTTCGTCAAAGGTGAGATCAAGTCTCCACGAGACTCGGAAGGCCATGGAACCCACACTGCATCAACGGCGGCAGGAAACTTAGTTAGCAAAGCAAGCCTATTTGGTTTGGGGTTGGGGACAGCAAGAGGAGGGGTCCCAGCAGCACGTATTGCAGTGTACAAAGCATGCTGGTCGACTGGTTGTAGTTTCGCTGATACTCTAGCGGCATTTGATGATGCGATCGCAGATGGTGTCGACATAATATCTGCCTCCCTCGGGCCGACGTCTCCGGACGATTATTTCAGGACACCAGTTACCATTGGAGCCTTTCACGCTTTAAGAAAAGGAATTCTGACTTCCACTGCTGCTGGGAACGACGGTCCAGCCCTCAAGACCATCACAGTCTTTGCGCCATGGTGTCTTTCTGTGGCTGCAACCACCATAGATCGGGAGTTTGTTACCAAGGTTCAATTGGGTAACCAAAAAATCTATGAGGGAATTGTAACAAACACATTTGACCTCAAGGGCAAGTTCTATCCTCTAATATATGCTGGAGACGCACCCAATAGGACAGCAGGCTACGGCGGGTCTATATCTAAGACATGCAAACCAGGCACGTTAGACCACAATTTGGTGAAGGGCAAGATTGTTCTTTGCGATGGGACGACTGGCTATGGGGCCTATTTTGCAGGTGCAGTTGGAGTTATTTTGCAAAGCCGACCTGTTGCAGATGTGCTTGACCCTCTTCCCATGCCTGCCTCTTGCCTTGGTTTGGATAGTGGTAACAGCATTTACTATTACATTACCTCAACAAGGAACCCAACTGCAACAATTTTTAAAAGTACTGAGGATATTGACACACTGTCCCCATATGTGCCATCCTTCTCATCAAGGGGTCCTAATCCAGTCAGTCCTAACATTCTCAAGCCAGATATAGCTGCTCCGGGAGCTTCTATTTTGGCATCATGGCCTCCAATTGCCCCGGTTTCGGATTATCCAGGAGATGACAGAGTAGCATCATACAATGTAATCTCAGGGACATCAATGGCTTGCCCACATGCCACAGGGATAGCTGCATACGTCAAATCATTTCACCCCAATTGGACTCCTGCTGCTATCCAATCTGCTCTTATTACCACCGCTAAACCCTTGAGTCCCGATCTTAACCCTGAAGCCGAATTTGCATATGGAGCTGGCCAAATTGACCCTGTTAGGGCTCCATATCCTGGTTTGGTATATGATGCTACTGAACTGGACTACATAGAATTTTTGTGTACACAAGGCTATAGTACTAAATTATTGCAATCTATTACCGGGCACAAGAGTTGCTGCTCATCAAAAACTAATTATGGAGCACTCAGTGATAATTTGAATTATCCTTCTTTTGCACTTTCCTCCTCTAATCCAAACTCCATCAGTGGGGTTTTCAATAGGACAGCCACAAATGTTGGATCACCAAGGTCCACATATAAAGCTAAAGTGATCGGTGCAACGAAAGGACTTGAAATCAAAGTTAATCCAAGCATTCTATCGTTCTCATCTCTCGGGCAGAAGCTATCCTTTCAGGTCACGGTAAAAGGGTCAATTCATCACAAATCCCGTGTCTCTACTTCTCTGGTGTGGGATGATGGTACTTTCCAAGTGAGGAGCCCCATTGTTGTCTATTCTATATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

775

Amino Acids

82.94

Weight (kDa)

8.65

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1328
AatII GACGTC 1 cut(s) 935
Acc36I ACCTGC 1 cut(s) 1328
AccB7I CCANNNNNTGG 2 cut(s) 1279, 1874
AccI GTMKAC 3 cut(s) 258, 842, 903
AccII CGCG 1 cut(s) 85
AccIII TCCGGA 2 cut(s) 172, 937
AciI CCGC 5 cut(s) 655, 737, 872, 1233, 1788
AclWI GGATC 1 cut(s) 2107
AcoI YGGCCR 2 cut(s) 533, 1843
AcsI RAATTY 3 cut(s) 996, 1826, 1913
AcuI CTGAAG 2 cut(s) 535, 1839
AcyI GRCGYC 1 cut(s) 932
AdeI CACNNNGTG 1 cut(s) 2009
AfaI GTAC 8 cut(s) 30, 136, 200, 827, 1492, 1924, 1939, 2281
AflIII ACRYGT 1 cut(s) 131
AgsI TTSAA 7 cut(s) 269, 337, 494, 1118, 2020, 2077, 2153
AhdI GACNNNNNGTC 1 cut(s) 1510
AjnI CCWGG 3 cut(s) 1258, 1645, 1873
Alw21I GWGCWC 2 cut(s) 646, 2005
Alw26I GTCTC 6 cut(s) 46, 693, 696, 939, 1197, 2257
AlwI GGATC 1 cut(s) 2107
Ama87I CYCGRG 2 cut(s) 923, 2192
Aor13HI TCCGGA 2 cut(s) 172, 937
AoxI GGCC 7 cut(s) 124, 533, 709, 926, 1326, 1616, 1843
ApoI RAATTY 3 cut(s) 996, 1826, 1913
ArsI GACNNNNNNTTYG 2 cut(s) 2235, 2267
AsiSI GCGATCGC 1 cut(s) 891
AspLEI GCGC 1 cut(s) 1060
AspS9I GGNCC 7 cut(s) 569, 802, 926, 1028, 1326, 1544, 2109
AsuC2I CCSGG 4 cut(s) 562, 1593, 1631, 1963
AsuHPI GGTGA 5 cut(s) 526, 692, 1294, 1733, 2094
AvaI CYCGRG 2 cut(s) 923, 2192
AvaII GGWCC 5 cut(s) 569, 802, 1028, 1544, 2109
BaeGI GKGCMC 1 cut(s) 1968
BalI TGGCCA 1 cut(s) 1845
BanII GRGCYC 2 cut(s) 1867, 2300
BarI GAAGNNNNNNTAC 2 cut(s) 249, 281
BauI CACGAG 1 cut(s) 696
Bbv12I GWGCWC 2 cut(s) 646, 2005
BccI CCATC 7 cut(s) 21, 890, 1052, 1301, 1534, 2069, 2270
BceAI ACGGC 2 cut(s) 750, 1246
BcgI CGANNNNNNTGC 2 cut(s) 822, 856
BciT130I CCWGG 3 cut(s) 1260, 1647, 1875
BciVI GTATCC 1 cut(s) 169
BcnI CCSGG 4 cut(s) 562, 1593, 1631, 1963
BcoDI GTCTC 6 cut(s) 46, 693, 696, 939, 1197, 2257
BfaI CTAG 3 cut(s) 38, 143, 869
BfmI CTRYAG 1 cut(s) 1933
BfuAI ACCTGC 1 cut(s) 1328
BfuI GTATCC 1 cut(s) 169
BmcAI AGTACT 2 cut(s) 1492, 1939
Bme1390I CCNGG 7 cut(s) 562, 1260, 1593, 1631, 1647, 1875, 1963
Bme18I GGWCC 5 cut(s) 569, 802, 1028, 1544, 2109
BmeRI GACNNNNNGTC 1 cut(s) 1510
BmeT110I CYCGRG 2 cut(s) 923, 2192
BmgT120I GGNCC 7 cut(s) 569, 802, 926, 1028, 1326, 1544, 2109
BmiI GGNNCC 9 cut(s) 718, 803, 804, 973, 1327, 1466, 1545, 1866, 2297
BmrFI CCNGG 7 cut(s) 562, 1260, 1593, 1631, 1647, 1875, 1963
BmsI GCATC 7 cut(s) 154, 547, 737, 874, 1619, 1673, 1879
BpmI CTGGAG 2 cut(s) 546, 1221
BpuEI CTTGAG 4 cut(s) 1022, 1154, 1556, 1819
BpuMI CCSGG 4 cut(s) 562, 1593, 1631, 1963
BsaAI YACGTR 1 cut(s) 815
BsaBI GATNNNNATC 1 cut(s) 168
BsaHI GRCGYC 1 cut(s) 932
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 9 cut(s) 77, 601, 712, 922, 1061, 1110, 1414, 1629, 2105
BsaWI WCCGGW 2 cut(s) 172, 937
Bse1I ACTGG 6 cut(s) 529, 850, 959, 1321, 1553, 1905
Bse8I GATNNNNATC 1 cut(s) 168
BseAI TCCGGA 2 cut(s) 172, 937
BseBI CCWGG 3 cut(s) 1260, 1647, 1875
BseDI CCNNGG 9 cut(s) 77, 601, 712, 922, 1061, 1110, 1414, 1629, 2105
BseGI GGATG 4 cut(s) 169, 608, 1526, 2278
BseJI GATNNNNATC 1 cut(s) 168
BseMII CTCAG 3 cut(s) 1485, 1695, 2020
BseNI ACTGG 6 cut(s) 529, 850, 959, 1321, 1553, 1905
BseRI GAGGAG 3 cut(s) 810, 2035, 2308
BseSI GKGCMC 1 cut(s) 1968
BseYI CCCAGC 2 cut(s) 805, 1016
BsgI GTGCAG 2 cut(s) 299, 1362
Bsh1236I CGCG 1 cut(s) 85
Bsh1285I CGRYCG 1 cut(s) 891
BshFI GGCC 7 cut(s) 126, 535, 711, 928, 1328, 1618, 1845
BsiEI CGRYCG 1 cut(s) 891
BsiHKAI GWGCWC 2 cut(s) 646, 2005
BsiHKCI CYCGRG 2 cut(s) 923, 2192
BsiSI CCGG 7 cut(s) 173, 536, 561, 938, 1592, 1631, 1962
BslFI GGGAC 7 cut(s) 221, 788, 799, 1324, 1498, 1699, 1788
BsmAI GTCTC 6 cut(s) 46, 693, 696, 939, 1197, 2257
BsmBI CGTCTC 2 cut(s) 939, 1197
BsmFI GGGAC 7 cut(s) 221, 788, 799, 1324, 1498, 1699, 1788
BsmI GAATGC 1 cut(s) 2172
BsnI GGCC 7 cut(s) 126, 535, 711, 928, 1328, 1618, 1845
Bso31I GGTCTC 1 cut(s) 46
BsoBI CYCGRG 2 cut(s) 923, 2192
Bsp1286I GDGCHC 5 cut(s) 646, 1867, 1968, 2005, 2300
Bsp13I TCCGGA 2 cut(s) 172, 937
Bsp1407I TGTACA 3 cut(s) 198, 825, 1922
Bsp143I GATC 7 cut(s) 516, 684, 888, 1093, 1807, 2099, 2130
Bsp19I CCATGG 2 cut(s) 712, 1061
Bsp68I TCGCGA 1 cut(s) 85
BspACI CCGC 5 cut(s) 655, 737, 872, 1233, 1788
BspANI GGCC 7 cut(s) 126, 535, 711, 928, 1328, 1618, 1845
BspCNI CTCAG 3 cut(s) 1486, 1694, 2019
BspEI TCCGGA 2 cut(s) 172, 937
BspFNI CGCG 1 cut(s) 85
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 9 cut(s) 718, 803, 804, 973, 1327, 1466, 1545, 1866, 2297
BspMI ACCTGC 1 cut(s) 1328
BspPI GGATC 1 cut(s) 2107
BspTNI GGTCTC 1 cut(s) 46
BsrGI TGTACA 3 cut(s) 198, 825, 1922
BsrI ACTGG 6 cut(s) 529, 850, 959, 1321, 1553, 1905
BssECI CCNNGG 9 cut(s) 77, 601, 712, 922, 1061, 1110, 1414, 1629, 2105
BssMI GATC 7 cut(s) 516, 684, 888, 1093, 1807, 2099, 2130
BssNI GRCGYC 1 cut(s) 932
BssSI CACGAG 1 cut(s) 696
BssT1I CCWWGG 7 cut(s) 77, 601, 712, 1061, 1110, 1414, 2105
Bst2BI CACGAG 1 cut(s) 696
Bst2UI CCWGG 3 cut(s) 1260, 1647, 1875
Bst4CI ACNGT 6 cut(s) 490, 648, 1028, 1051, 1512, 2221
Bst6I CTCTTC 3 cut(s) 255, 473, 1398
BstACI GRCGYC 1 cut(s) 932
BstAPI GCANNNNNTGC 1 cut(s) 818
BstAUI TGTACA 3 cut(s) 198, 825, 1922
BstBAI YACGTR 1 cut(s) 815
BstC8I GCNNGC 8 cut(s) 8, 547, 763, 835, 1225, 1404, 1699, 1843
BstDEI CTNAG 5 cut(s) 748, 1245, 1494, 1681, 2006
BstDSI CCRYGG 2 cut(s) 712, 1061
BstEII GGTNACC 1 cut(s) 1124
BstENI CCTNNNNNAGG 1 cut(s) 1459
BstF5I GGATG 4 cut(s) 169, 608, 1526, 2278
BstFNI CGCG 1 cut(s) 85
BstHHI GCGC 1 cut(s) 1060
BstKTI GATC 7 cut(s) 519, 687, 891, 1096, 1810, 2102, 2133
BstMAI GTCTC 6 cut(s) 46, 693, 696, 939, 1197, 2257
BstMBI GATC 7 cut(s) 516, 684, 888, 1093, 1807, 2099, 2130
BstMCI CGRYCG 1 cut(s) 891
BstNI CCWGG 3 cut(s) 1260, 1647, 1875
BstNSI RCATGY 6 cut(s) 135, 265, 600, 837, 1254, 1709
BstPI GGTNACC 1 cut(s) 1124
BstSCI CCNGG 7 cut(s) 560, 1258, 1591, 1629, 1645, 1873, 1961
BstSFI CTRYAG 1 cut(s) 1933
BstSLI GKGCMC 1 cut(s) 1968
BstUI CGCG 1 cut(s) 85
BstXI CCANNNNNNTGG 1 cut(s) 585
BsuI GTATCC 1 cut(s) 169
BsuRI GGCC 7 cut(s) 126, 535, 711, 928, 1328, 1618, 1845
BtgI CCRYGG 2 cut(s) 712, 1061
BtgZI GCGATG 1 cut(s) 1320
BtsCI GGATG 4 cut(s) 169, 608, 1526, 2278
BtsI GCAGTG 3 cut(s) 723, 828, 1008
BtsIMutI CAGTG 9 cut(s) 486, 522, 559, 723, 828, 1008, 1508, 2014, 2071
BtuMI TCGCGA 1 cut(s) 85
BveI ACCTGC 1 cut(s) 1328
Cac8I GCNNGC 8 cut(s) 8, 547, 763, 835, 1225, 1404, 1699, 1843
CciI TCATGA 1 cut(s) 466
CfoI GCGC 1 cut(s) 1060
Cfr13I GGNCC 7 cut(s) 569, 802, 926, 1028, 1326, 1544, 2109
CseI GACGC 1 cut(s) 1214
Csp6I GTAC 8 cut(s) 29, 135, 199, 826, 1491, 1923, 1938, 2280
CspCI CAANNNNNGTGG 2 cut(s) 2077, 2112
CviQI GTAC 8 cut(s) 29, 135, 199, 826, 1491, 1923, 1938, 2280
DdeI CTNAG 5 cut(s) 748, 1245, 1494, 1681, 2006
DpnI GATC 7 cut(s) 518, 686, 890, 1095, 1809, 2101, 2132
DpnII GATC 7 cut(s) 516, 684, 888, 1093, 1807, 2099, 2130
DraI TTTAAA 1 cut(s) 1486
DraIII CACNNNGTG 1 cut(s) 2009
DriI GACNNNNNGTC 1 cut(s) 1510
EaeI YGGCCR 2 cut(s) 533, 1843
Eam1104I CTCTTC 3 cut(s) 255, 473, 1398
Eam1105I GACNNNNNGTC 1 cut(s) 1510
EarI CTCTTC 3 cut(s) 255, 473, 1398
Eco130I CCWWGG 7 cut(s) 77, 601, 712, 1061, 1110, 1414, 2105
Eco24I GRGCYC 2 cut(s) 1867, 2300
Eco31I GGTCTC 1 cut(s) 46
Eco32I GATATC 1 cut(s) 502
Eco47I GGWCC 5 cut(s) 569, 802, 1028, 1544, 2109
Eco57I CTGAAG 2 cut(s) 535, 1839
Eco88I CYCGRG 2 cut(s) 923, 2192
Eco91I GGTNACC 1 cut(s) 1124
EcoNI CCTNNNNNAGG 1 cut(s) 1459
EcoO109I RGGNCCY 3 cut(s) 802, 1326, 1544
EcoO65I GGTNACC 1 cut(s) 1124
EcoRI GAATTC 1 cut(s) 996
EcoRII CCWGG 3 cut(s) 1258, 1645, 1873
EcoRV GATATC 1 cut(s) 502
EcoT14I CCWWGG 7 cut(s) 77, 601, 712, 1061, 1110, 1414, 2105
EcoT38I GRGCYC 2 cut(s) 1867, 2300
ErhI CCWWGG 7 cut(s) 77, 601, 712, 1061, 1110, 1414, 2105
Esp3I CGTCTC 2 cut(s) 939, 1197
FalI AAGNNNNNCTT 4 cut(s) 317, 349, 2192, 2224
FaqI GGGAC 7 cut(s) 221, 788, 799, 1324, 1498, 1699, 1788
FauI CCCGC 1 cut(s) 1226
FauNDI CATATG 2 cut(s) 1519, 1834
FblI GTMKAC 3 cut(s) 258, 842, 903
FokI GGATG 4 cut(s) 156, 595, 1513, 2285
FriOI GRGCYC 2 cut(s) 1867, 2300
FspBI CTAG 3 cut(s) 38, 143, 869
GlaI GCGC 1 cut(s) 1059
GsaI CCCAGC 2 cut(s) 809, 1020
GsuI CTGGAG 2 cut(s) 546, 1221
HaeIII GGCC 7 cut(s) 126, 535, 711, 928, 1328, 1618, 1845
HapII CCGG 7 cut(s) 173, 536, 561, 938, 1592, 1631, 1962
HgaI GACGC 1 cut(s) 1214
HhaI GCGC 1 cut(s) 1060
Hin1I GRCGYC 1 cut(s) 932
Hin6I GCGC 1 cut(s) 1058
HinP1I GCGC 1 cut(s) 1058
HincII GTYRAC 2 cut(s) 843, 904
HindII GTYRAC 2 cut(s) 843, 904
HindIII AAGCTT 1 cut(s) 331
HinfI GANTC 4 cut(s) 539, 701, 1753, 1801
HpaII CCGG 7 cut(s) 173, 536, 561, 938, 1592, 1631, 1962
HphI GGTGA 5 cut(s) 526, 692, 1294, 1733, 2094
Hpy188I TCNGA 4 cut(s) 163, 706, 1002, 1639
Hpy188III TCNNGA 8 cut(s) 84, 173, 467, 938, 952, 1039, 1097, 1805
Hpy99I CGWCG 2 cut(s) 934, 1028
HpyAV CCTTC 6 cut(s) 128, 679, 701, 1279, 1540, 2037
HpyCH4III ACNGT 6 cut(s) 490, 648, 1028, 1051, 1512, 2221
HpyCH4IV ACGT 7 cut(s) 31, 273, 474, 814, 932, 1265, 1728
HpyF3I CTNAG 5 cut(s) 748, 1245, 1494, 1681, 2006
HpySE526I ACGT 7 cut(s) 31, 273, 474, 814, 932, 1265, 1728
Hsp92I GRCGYC 1 cut(s) 932
HspAI GCGC 1 cut(s) 1058
KflI GGGWCCC 1 cut(s) 802
Kpn2I TCCGGA 2 cut(s) 172, 937
Kzo9I GATC 7 cut(s) 516, 684, 888, 1093, 1807, 2099, 2130
LmnI GCTCC 9 cut(s) 435, 641, 971, 1594, 1595, 1838, 1870, 2000, 2295
LweI GCATC 7 cut(s) 154, 547, 737, 874, 1619, 1673, 1879
MaeI CTAG 3 cut(s) 38, 143, 869
MaeII ACGT 7 cut(s) 31, 273, 474, 814, 932, 1265, 1728
MaeIII GTNAC 7 cut(s) 151, 961, 1105, 1124, 1150, 1430, 2215
MalI GATC 7 cut(s) 518, 686, 890, 1095, 1809, 2101, 2132
MboI GATC 7 cut(s) 516, 684, 888, 1093, 1807, 2099, 2130
MboII GAAGA 4 cut(s) 242, 377, 490, 1385
MfeI CAATTG 3 cut(s) 1118, 1623, 1747
MhlI GDGCHC 5 cut(s) 646, 1867, 1968, 2005, 2300
MlsI TGGCCA 1 cut(s) 1845
MluNI TGGCCA 1 cut(s) 1845
MlyI GAGTC 3 cut(s) 695, 1747, 1810
MmeI TCCRAC 2 cut(s) 1327, 2077
Mox20I TGGCCA 1 cut(s) 1845
MroI TCCGGA 2 cut(s) 172, 937
MscI TGGCCA 1 cut(s) 1845
MseI TTAA 5 cut(s) 986, 1485, 1812, 2163, 2323
MslI CAYNNNNRTG 1 cut(s) 162
Msp20I TGGCCA 1 cut(s) 1845
MspI CCGG 7 cut(s) 173, 536, 561, 938, 1592, 1631, 1962
MspR9I CCNGG 7 cut(s) 562, 1260, 1593, 1631, 1647, 1875, 1963
MunI CAATTG 3 cut(s) 1118, 1623, 1747
Mva1269I GAATGC 1 cut(s) 2172
MvaI CCWGG 3 cut(s) 1260, 1647, 1875
MvnI CGCG 1 cut(s) 85
NciI CCSGG 4 cut(s) 562, 1593, 1631, 1963
NcoI CCATGG 2 cut(s) 712, 1061
NdeI CATATG 2 cut(s) 1519, 1834
NdeII GATC 7 cut(s) 516, 684, 888, 1093, 1807, 2099, 2130
NlaIV GGNNCC 9 cut(s) 718, 803, 804, 973, 1327, 1466, 1545, 1866, 2297
NmeAIII GCCGAG 1 cut(s) 102
NmuCI GTSAC 2 cut(s) 151, 2215
NruI TCGCGA 1 cut(s) 85
NspI RCATGY 6 cut(s) 135, 265, 600, 837, 1254, 1709
PaeI GCATGC 1 cut(s) 837
PagI TCATGA 1 cut(s) 466
PaqCI CACCTGC 1 cut(s) 1328
PciI ACATGT 1 cut(s) 131
PctI GAATGC 1 cut(s) 2172
PfeI GAWTC 1 cut(s) 539
PflMI CCANNNNNTGG 2 cut(s) 1279, 1874
PfoI TCCNGGA 2 cut(s) 1591, 1645
Ple19I CGATCG 1 cut(s) 891
PleI GAGTC 3 cut(s) 695, 1747, 1809
PpsI GAGTC 3 cut(s) 695, 1747, 1809
Ppu21I YACGTR 1 cut(s) 815
PpuMI RGGWCCY 2 cut(s) 802, 1544
PscI ACATGT 1 cut(s) 131
Psp5II RGGWCCY 2 cut(s) 802, 1544
Psp6I CCWGG 3 cut(s) 1258, 1645, 1873
PspEI GGTNACC 1 cut(s) 1124
PspFI CCCAGC 2 cut(s) 805, 1016
PspGI CCWGG 3 cut(s) 1258, 1645, 1873
PspN4I GGNNCC 9 cut(s) 718, 803, 804, 973, 1327, 1466, 1545, 1866, 2297
PspPI GGNCC 7 cut(s) 569, 802, 926, 1028, 1326, 1544, 2109
PspPPI RGGWCCY 2 cut(s) 802, 1544
PsrI GAACNNNNNNTAC 2 cut(s) 294, 326
PvuI CGATCG 1 cut(s) 891
RgaI GCGATCGC 1 cut(s) 891
RruI TCGCGA 1 cut(s) 85
RsaI GTAC 8 cut(s) 30, 136, 200, 827, 1492, 1924, 1939, 2281
RsaNI GTAC 8 cut(s) 29, 135, 199, 826, 1491, 1923, 1938, 2280
RseI CAYNNNNRTG 1 cut(s) 162
SalI GTCGAC 2 cut(s) 841, 902
SaqAI TTAA 5 cut(s) 986, 1485, 1812, 2163, 2323
Sau3AI GATC 7 cut(s) 516, 684, 888, 1093, 1807, 2099, 2130
Sau96I GGNCC 7 cut(s) 569, 802, 926, 1028, 1326, 1544, 2109
ScaI AGTACT 2 cut(s) 1492, 1939
SchI GAGTC 3 cut(s) 695, 1747, 1810
ScrFI CCNGG 7 cut(s) 562, 1260, 1593, 1631, 1647, 1875, 1963
SduI GDGCHC 5 cut(s) 646, 1867, 1968, 2005, 2300
SfaAI GCGATCGC 1 cut(s) 891
SfaNI GCATC 7 cut(s) 154, 547, 737, 874, 1619, 1673, 1879
SfcI CTRYAG 1 cut(s) 1933
SgfI GCGATCGC 1 cut(s) 891
SinI GGWCC 5 cut(s) 569, 802, 1028, 1544, 2109
SmiMI CAYNNNNRTG 1 cut(s) 162
SmlI CTYRAG 4 cut(s) 1037, 1169, 1571, 1798
SmoI CTYRAG 4 cut(s) 1037, 1169, 1571, 1798
SphI GCATGC 1 cut(s) 837
SsiI CCGC 5 cut(s) 655, 737, 872, 1233, 1788
SspI AATATT 1 cut(s) 292
SspMI CTAG 3 cut(s) 38, 143, 869
StyD4I CCNGG 7 cut(s) 560, 1258, 1591, 1629, 1645, 1873, 1961
StyI CCWWGG 7 cut(s) 77, 601, 712, 1061, 1110, 1414, 2105
TaaI ACNGT 6 cut(s) 490, 648, 1028, 1051, 1512, 2221
TaiI ACGT 7 cut(s) 34, 276, 477, 817, 935, 1268, 1731
TaqI TCGA 4 cut(s) 222, 519, 842, 903
TatI WGTACW 6 cut(s) 134, 198, 825, 1490, 1922, 1937
TauI GCSGC 2 cut(s) 740, 875
TfiI GAWTC 1 cut(s) 539
Tru1I TTAA 5 cut(s) 986, 1485, 1812, 2163, 2323
Tru9I TTAA 5 cut(s) 986, 1485, 1812, 2163, 2323
TscAI CASTG 9 cut(s) 493, 529, 559, 730, 828, 1015, 1515, 2014, 2071
TseFI GTSAC 2 cut(s) 151, 2215
Tsp45I GTSAC 2 cut(s) 151, 2215
TspDTI ATGAA 5 cut(s) 242, 445, 455, 483, 2225
TspRI CASTG 9 cut(s) 493, 529, 559, 730, 828, 1015, 1515, 2014, 2071
Van91I CCANNNNNTGG 2 cut(s) 1279, 1874
VpaK11BI GGWCC 5 cut(s) 569, 802, 1028, 1544, 2109
XagI CCTNNNNNAGG 1 cut(s) 1459
XapI RAATTY 3 cut(s) 996, 1826, 1913
XceI RCATGY 6 cut(s) 135, 265, 600, 837, 1254, 1709
XcmI CCANNNNNNNNNTGG 2 cut(s) 582, 1117
XmiI GTMKAC 3 cut(s) 258, 842, 903
XspI CTAG 3 cut(s) 38, 143, 869
ZraI GACGTC 1 cut(s) 933
ZrmI AGTACT 2 cut(s) 1492, 1939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.