Rw1G031700

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000390
Physical Location & Seq
Forward (+)
59866687 .. 59871044
4358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G031700.1

Sequence Viewer

Length: 2217 bp
ATGGCTCTTCGATGGTTTCTCCTTCTCAACCTCATTTGCAATATTCTACTGCTTGTTGATGTTACTCACTCAGTTGCTCAGGATGCCCGAAAGACTTATGTTGTGTATATGGGCGACAAGCCAAAAAACGGGCCCATATTACCTTATCTTCATGTGAATATGCTACAAGACGTAGTTGACAGTAGCAACATTGATATTGCACATGAAGATCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATGATTGGAATGGATGGTGTAGTATCTGTCTTCCCAAGCAAACAAAGGAGTGCCAAAACAACAAAGTCATGGAACTTTCTTGGATTTCCAGAAACAGTAAAGAGAAGCGCCCTTGAAAGGGATATCATCATAGGTGTGATGGACAGTGGAATTTGGCCAGAGTCAAACAGCTTTAGTGATGTTGGTTTTGGTCCTCCACCCAAGAAATGGAAAGGCAAATGTCAAGGCCATGGAAATCTTACTTGTAACAATAAAATCATCGGAGCACAGTATTACCGTGCTTCTCGATCCTTCAAAGAAGATGTTGAGTCTCCAAGAGACTCAAGCGGTCATGGAACTCACACCGCATCAACAGCAGCTGGGAACACAGTGAGTAAGGCAAGCCTAAATGGTTTAGGGCTGGGAACAGCAAGAGGAGGGGTACCATCAGCACGTATTTCTGTGTACAAAATATGTTGGGGCAGCAGCATCAGTTGTGATGATGCTGACATTCTAGCGGCATTCGATGATGCCATTGCTGACGGTGTTGACATTCTCTCTATTTCCGTTGGAGGCCTTATTCCATCTGATTATTTTAATGATCCATTTGCAATTGGGTCATTTCACGCTACCAGAAATGGGATATTAGTTTCCATGGCCGCTGGTAACGAAGGTCCGGGAGCAAAAACTGTGACAAACTTTGCACCATGGCAACTTTCTGTGGCTGCTACCACCATAAACCGCCAGTTCATCACTAAGGTTCAATTGGGTAACGGTAAGCTCTATGAGGGACTCATACCAAACATATATGACCTCCGGGGTGAATTCTATCCTTTAATTTATGGTGGAGATGCACCCAATAAAAAAGCAGGTGTTGATGGGACTATCTCCAGGTTTTGTGTAAGAGATGCCTTAGAAGAAGATTTGATTAAAGGTAAAATTGTGCTTTGTGATGGCCCTATCGACGGGGCTGGGGTTGGGGCCATATTAGGTGGTGCAGCTGGAATTGTTCTGACAGGAAAAAAAGTTGCTGATGAAGCGCTTGGCCCTTATCTCGTGCCTGCATCTTACGTCGGATTGGAAGAAAACAGCGACATTTACAAATACATAAAATCAACAAGGAACGCAACTGCAACTATTTGGAAAAGTGAAGAGATTAGTGATGTATTGGCTCCATATGTGCCCTCCTACTCATCGAGGGGCCCAAATCCGATCAATCCCAACATTCTCAAGCCAGATTTAGCGGCTCCAGGAACTTACATTCTGGCAGCATATCCCCCAAGTGGTGGTCGCAGTAGAGCTAGATACTATCTAGAAACCGGGACATCAATGGCATGTCCCCATGCTACAGCCGTAGCTGCATATGTCAAATCATTTCACCCTAAATGGTCACCGGCTGCTATCCAATCGGCACTTATCACTACTGCTAAACCTATGAGTGCCAAAACTAGCTCTGACGCTGAATTTGCATACGGAGCTGGCCTAATAAACCCTTCTAAGGCGCCATATCCTGGTTTGGTGTACGATCTTGATGAACAAGACTACATAGATTTTTTGTGTTCACAAGGATATAGTGGTAAACTATTGCAAAGCATAACCAGGGACAAGGCTAGTTGCTCATCAAAATCTATTAATGGAACAACTAATGACATGAACTATCCTTCTTTTGCACTTTCCATCAAGGATACGAAATTTGTCAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCGTCAAAATCCACATATAGAGCTAAAGTGGTGGCTCCATTGGGACTCAAAATCAATGTGAATCCAAGTGTGCTATCGTTCACATCTCTCGGGCAAAAGAAATCTTTTGTTGTCACTATAAATGGGCCGATTGAGAAATCAAATCTAGTCTCTGCGTCTTTGGTGTGGGATGATGGTGCTTTCCAAGTCAGGAGCCCCATCGTTGTCTATGTTGTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

738

Amino Acids

79.04

Weight (kDa)

8.53

Isoelectric Point (pI)

35.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 32 - 111 6.7e-13 Peptidase inhibitor I9
Peptidase_S8 PF00082 134 - 580 3.5e-42 Subtilase family
fn3_6 PF17766 637 - 734 9.9e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1121
Acc36I ACCTGC 1 cut(s) 1121
Acc65I GGTACC 1 cut(s) 703
AccB1I GGYRCC 2 cut(s) 703, 1762
AccB7I CCANNNNNTGG 3 cut(s) 489, 1547, 1772
AciI CCGC 6 cut(s) 609, 627, 779, 921, 1003, 1505
AclWI GGATC 3 cut(s) 564, 857, 2002
AcoI YGGCCR 2 cut(s) 437, 918
AcsI RAATTY 4 cut(s) 432, 1085, 1724, 1952
AcyI GRCGYC 1 cut(s) 1763
AfaI GTAC 3 cut(s) 705, 728, 1784
AfeI AGCGCT 1 cut(s) 1302
AgsI TTSAA 4 cut(s) 241, 398, 577, 1025
AjnI CCWGG 4 cut(s) 1151, 1510, 1771, 1859
Alw21I GWGCWC 1 cut(s) 550
Alw26I GTCTC 3 cut(s) 594, 597, 2149
AlwI GGATC 3 cut(s) 564, 857, 2002
AlwNI CAGNNNCTG 1 cut(s) 641
Ama87I CYCGRG 1 cut(s) 2084
Aor51HI AGCGCT 1 cut(s) 1302
ApaI GGGCCC 2 cut(s) 135, 1466
ApeKI GCWGC 9 cut(s) 251, 638, 744, 747, 986, 1259, 1529, 1619, 1658
ApoI RAATTY 4 cut(s) 432, 1085, 1724, 1952
ArsI GACNNNNNNTTYG 2 cut(s) 1709, 1741
AseI ATTAAT 1 cut(s) 1893
Asp718I GGTACC 1 cut(s) 703
AspLEI GCGC 3 cut(s) 392, 1303, 1765
AsuC2I CCSGG 3 cut(s) 939, 1079, 1582
AsuHPI GGTGA 3 cut(s) 1094, 1631, 1644
AvaI CYCGRG 1 cut(s) 2084
AvaII GGWCC 2 cut(s) 473, 935
BaeGI GKGCMC 3 cut(s) 135, 1446, 1466
BalI TGGCCA 1 cut(s) 439
BanI GGYRCC 2 cut(s) 703, 1762
BanII GRGCYC 3 cut(s) 135, 1466, 2192
BauI CACGAG 1 cut(s) 1316
BbsI GAAGAC 2 cut(s) 304, 1960
Bbv12I GWGCWC 1 cut(s) 550
BbvI GCAGC 9 cut(s) 238, 650, 756, 759, 973, 1271, 1541, 1606, 1645
BceAI ACGGC 1 cut(s) 1598
BciT130I CCWGG 4 cut(s) 1153, 1512, 1773, 1861
BciVI GTATCC 1 cut(s) 1939
BcnI CCSGG 3 cut(s) 939, 1079, 1582
BcoDI GTCTC 3 cut(s) 594, 597, 2149
BfaI CTAG 6 cut(s) 776, 1563, 1574, 1710, 1872, 2141
BfmI CTRYAG 1 cut(s) 1608
BfoI RGCGCY 3 cut(s) 393, 1304, 1766
BfuAI ACCTGC 1 cut(s) 1121
BfuI GTATCC 1 cut(s) 1939
BglII AGATCT 1 cut(s) 208
Bme1390I CCNGG 7 cut(s) 939, 1079, 1153, 1512, 1582, 1773, 1861
Bme18I GGWCC 2 cut(s) 473, 935
BmeT110I CYCGRG 1 cut(s) 2084
BmrFI CCNGG 7 cut(s) 939, 1079, 1153, 1512, 1582, 1773, 1861
BmsI GCATC 8 cut(s) 73, 638, 754, 759, 781, 1102, 1159, 1334
BpiI GAAGAC 2 cut(s) 304, 1960
BpmI CTGGAG 2 cut(s) 1135, 1494
Bpu10I CCTNAGC 1 cut(s) 78
BpuEI CTTGAG 2 cut(s) 589, 1475
BpuMI CCSGG 3 cut(s) 939, 1079, 1582
BsaAI YACGTR 1 cut(s) 716
BsaHI GRCGYC 1 cut(s) 1763
BsaJI CCNNGG 5 cut(s) 511, 915, 968, 1078, 1860
Bse118I RCCGGY 1 cut(s) 1654
Bse1I ACTGG 1 cut(s) 1006
Bse3DI GCAATG 2 cut(s) 261, 795
BseBI CCWGG 4 cut(s) 1153, 1512, 1773, 1861
BseDI CCNNGG 5 cut(s) 511, 915, 968, 1078, 1860
BseGI GGATG 3 cut(s) 88, 301, 2170
BseMI GCAATG 2 cut(s) 261, 795
BseMII CTCAG 2 cut(s) 84, 92
BseNI ACTGG 1 cut(s) 1006
BseRI GAGGAG 1 cut(s) 711
BseSI GKGCMC 3 cut(s) 135, 1446, 1466
BseXI GCAGC 9 cut(s) 238, 650, 756, 759, 973, 1271, 1541, 1606, 1645
BseYI CCCAGC 3 cut(s) 641, 682, 1232
BsgI GTGCAG 1 cut(s) 1278
BshNI GGYRCC 2 cut(s) 703, 1762
BsiHKAI GWGCWC 1 cut(s) 550
BsiHKCI CYCGRG 1 cut(s) 2084
BsiSI CCGG 4 cut(s) 938, 1078, 1581, 1655
BslFI GGGAC 6 cut(s) 1065, 1156, 1584, 1597, 1877, 2052
BsmAI GTCTC 3 cut(s) 594, 597, 2149
BsmFI GGGAC 6 cut(s) 1065, 1156, 1584, 1597, 1877, 2052
BsmI GAATGC 1 cut(s) 782
BsoBI CYCGRG 1 cut(s) 2084
Bsp120I GGGCCC 2 cut(s) 131, 1462
Bsp1286I GDGCHC 5 cut(s) 135, 550, 1446, 1466, 2192
Bsp1407I TGTACA 1 cut(s) 726
Bsp143I GATC 6 cut(s) 208, 569, 862, 1473, 1786, 1994
Bsp19I CCATGG 3 cut(s) 511, 915, 968
BspACI CCGC 6 cut(s) 609, 627, 779, 921, 1003, 1505
BspCNI CTCAG 2 cut(s) 83, 91
BspMI ACCTGC 1 cut(s) 1121
BspPI GGATC 3 cut(s) 564, 857, 2002
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 2 cut(s) 703, 1762
BsrDI GCAATG 2 cut(s) 261, 795
BsrFI RCCGGY 1 cut(s) 1654
BsrGI TGTACA 1 cut(s) 726
BsrI ACTGG 1 cut(s) 1006
BssAI RCCGGY 1 cut(s) 1654
BssECI CCNNGG 5 cut(s) 511, 915, 968, 1078, 1860
BssMI GATC 6 cut(s) 208, 569, 862, 1473, 1786, 1994
BssNI GRCGYC 1 cut(s) 1763
BssSI CACGAG 1 cut(s) 1316
BssT1I CCWWGG 3 cut(s) 511, 915, 968
Bst2BI CACGAG 1 cut(s) 1316
Bst2UI CCWGG 4 cut(s) 1153, 1512, 1773, 1861
Bst6I CTCTTC 2 cut(s) 12, 1407
BstACI GRCGYC 1 cut(s) 1763
BstAUI TGTACA 1 cut(s) 726
BstBAI YACGTR 1 cut(s) 716
BstC8I GCNNGC 3 cut(s) 664, 1323, 1741
BstDEI CTNAG 5 cut(s) 70, 78, 1017, 1174, 1758
BstDSI CCRYGG 3 cut(s) 511, 915, 968
BstEII GGTNACC 1 cut(s) 1650
BstF5I GGATG 3 cut(s) 88, 301, 2170
BstH2I RGCGCY 3 cut(s) 393, 1304, 1766
BstHHI GCGC 3 cut(s) 392, 1303, 1765
BstKTI GATC 6 cut(s) 211, 572, 865, 1476, 1789, 1997
BstMAI GTCTC 3 cut(s) 594, 597, 2149
BstMBI GATC 6 cut(s) 208, 569, 862, 1473, 1786, 1994
BstMWI GCNNNNNNNGC 7 cut(s) 83, 265, 635, 1298, 1619, 1727, 1736
BstNI CCWGG 4 cut(s) 1153, 1512, 1773, 1861
BstNSI RCATGY 1 cut(s) 1599
BstPI GGTNACC 1 cut(s) 1650
BstSCI CCNGG 7 cut(s) 937, 1077, 1151, 1510, 1580, 1771, 1859
BstSFI CTRYAG 1 cut(s) 1608
BstSLI GKGCMC 3 cut(s) 135, 1446, 1466
BstV1I GCAGC 9 cut(s) 238, 650, 756, 759, 973, 1271, 1541, 1606, 1645
BstV2I GAAGAC 2 cut(s) 304, 1960
BstX2I RGATCY 1 cut(s) 208
BstYI RGATCY 1 cut(s) 208
BsuI GTATCC 1 cut(s) 1939
BtgI CCRYGG 3 cut(s) 511, 915, 968
BtsCI GGATG 3 cut(s) 88, 301, 2170
BtsIMutI CAGTG 2 cut(s) 433, 657
BveI ACCTGC 1 cut(s) 1121
Cac8I GCNNGC 3 cut(s) 664, 1323, 1741
CaiI CAGNNNCTG 1 cut(s) 641
CfoI GCGC 3 cut(s) 392, 1303, 1765
Cfr10I RCCGGY 1 cut(s) 1654
CseI GACGC 2 cut(s) 1727, 2139
Csp6I GTAC 3 cut(s) 704, 727, 1783
CviQI GTAC 3 cut(s) 704, 727, 1783
DdeI CTNAG 5 cut(s) 70, 78, 1017, 1174, 1758
DinI GGCGCC 1 cut(s) 1764
DpnI GATC 6 cut(s) 210, 571, 864, 1475, 1788, 1996
DpnII GATC 6 cut(s) 208, 569, 862, 1473, 1786, 1994
EaeI YGGCCR 2 cut(s) 437, 918
Eam1104I CTCTTC 2 cut(s) 12, 1407
EarI CTCTTC 2 cut(s) 12, 1407
Eco130I CCWWGG 3 cut(s) 511, 915, 968
Eco147I AGGCCT 1 cut(s) 837
Eco24I GRGCYC 3 cut(s) 135, 1466, 2192
Eco32I GATATC 1 cut(s) 406
Eco47I GGWCC 2 cut(s) 473, 935
Eco47III AGCGCT 1 cut(s) 1302
Eco88I CYCGRG 1 cut(s) 2084
Eco91I GGTNACC 1 cut(s) 1650
EcoO109I RGGNCCY 1 cut(s) 1462
EcoO65I GGTNACC 1 cut(s) 1650
EcoRI GAATTC 1 cut(s) 1085
EcoRII CCWGG 4 cut(s) 1151, 1510, 1771, 1859
EcoRV GATATC 1 cut(s) 406
EcoT14I CCWWGG 3 cut(s) 511, 915, 968
EcoT38I GRGCYC 3 cut(s) 135, 1466, 2192
EgeI GGCGCC 1 cut(s) 1764
EheI GGCGCC 1 cut(s) 1764
ErhI CCWWGG 3 cut(s) 511, 915, 968
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FaqI GGGAC 6 cut(s) 1065, 1156, 1584, 1597, 1877, 2052
FauNDI CATATG 2 cut(s) 1438, 1624
FokI GGATG 3 cut(s) 95, 308, 2177
FriOI GRGCYC 3 cut(s) 135, 1466, 2192
FspBI CTAG 6 cut(s) 776, 1563, 1574, 1710, 1872, 2141
GlaI GCGC 3 cut(s) 391, 1302, 1764
GsaI CCCAGC 3 cut(s) 645, 686, 1236
GsuI CTGGAG 2 cut(s) 1135, 1494
HaeII RGCGCY 3 cut(s) 393, 1304, 1766
HapII CCGG 4 cut(s) 938, 1078, 1581, 1655
HgaI GACGC 2 cut(s) 1727, 2139
HhaI GCGC 3 cut(s) 392, 1303, 1765
Hin1I GRCGYC 1 cut(s) 1763
Hin6I GCGC 3 cut(s) 390, 1301, 1763
HinP1I GCGC 3 cut(s) 390, 1301, 1763
HincII GTYRAC 2 cut(s) 178, 811
HindII GTYRAC 2 cut(s) 178, 811
HinfI GANTC 6 cut(s) 443, 590, 602, 1053, 2040, 2056
HpaII CCGG 4 cut(s) 938, 1078, 1581, 1655
HphI GGTGA 3 cut(s) 1094, 1631, 1644
Hpy166II GTNNAC 7 cut(s) 178, 727, 811, 1783, 1823, 1841, 2076
Hpy188I TCNGA 6 cut(s) 545, 850, 1275, 1337, 1473, 1717
Hpy188III TCNNGA 6 cut(s) 80, 371, 567, 1574, 1790, 2185
Hpy8I GTNNAC 7 cut(s) 178, 727, 811, 1783, 1823, 1841, 2076
Hpy99I CGWCG 2 cut(s) 1229, 1337
HpyAV CCTTC 5 cut(s) 32, 583, 926, 1764, 1932
HpyCH4IV ACGT 3 cut(s) 171, 715, 1332
HpyF10VI GCNNNNNNNGC 7 cut(s) 83, 265, 635, 1298, 1619, 1727, 1736
HpyF3I CTNAG 5 cut(s) 70, 78, 1017, 1174, 1758
HpySE526I ACGT 3 cut(s) 171, 715, 1332
Hsp92I GRCGYC 1 cut(s) 1763
HspAI GCGC 3 cut(s) 390, 1301, 1763
KasI GGCGCC 1 cut(s) 1762
KpnI GGTACC 1 cut(s) 707
Kzo9I GATC 6 cut(s) 208, 569, 862, 1473, 1786, 1994
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 7 cut(s) 545, 941, 1438, 1513, 1736, 2035, 2187
Lsp1109I GCAGC 9 cut(s) 238, 650, 756, 759, 973, 1271, 1541, 1606, 1645
LweI GCATC 8 cut(s) 73, 638, 754, 759, 781, 1102, 1159, 1334
MaeI CTAG 6 cut(s) 776, 1563, 1574, 1710, 1872, 2141
MaeII ACGT 3 cut(s) 171, 715, 1332
MaeIII GTNAC 8 cut(s) 61, 527, 926, 952, 1031, 1650, 1981, 2107
MalI GATC 6 cut(s) 210, 571, 864, 1475, 1788, 1996
MboI GATC 6 cut(s) 208, 569, 862, 1473, 1786, 1994
MfeI CAATTG 2 cut(s) 873, 1025
MflI RGATCY 1 cut(s) 208
MhlI GDGCHC 5 cut(s) 135, 550, 1446, 1466, 2192
MlsI TGGCCA 1 cut(s) 439
MluCI AATT 9 cut(s) 432, 873, 1025, 1085, 1098, 1200, 1266, 1724, 1952
MluNI TGGCCA 1 cut(s) 439
Mly113I GGCGCC 1 cut(s) 1763
MlyI GAGTC 5 cut(s) 452, 596, 599, 1047, 2034
MmeI TCCRAC 3 cut(s) 811, 1315, 1972
MnlI CCTC 9 cut(s) 41, 486, 689, 692, 827, 1042, 1085, 1452, 1456
Mox20I TGGCCA 1 cut(s) 439
MscI TGGCCA 1 cut(s) 439
MseI TTAA 5 cut(s) 858, 1097, 1191, 1893, 2215
MslI CAYNNNNRTG 1 cut(s) 416
Msp20I TGGCCA 1 cut(s) 439
MspA1I CMGCKG 3 cut(s) 641, 923, 1262
MspI CCGG 4 cut(s) 938, 1078, 1581, 1655
MspR9I CCNGG 7 cut(s) 939, 1079, 1153, 1512, 1582, 1773, 1861
MunI CAATTG 2 cut(s) 873, 1025
Mva1269I GAATGC 1 cut(s) 782
MvaI CCWGG 4 cut(s) 1153, 1512, 1773, 1861
MwoI GCNNNNNNNGC 7 cut(s) 83, 265, 635, 1298, 1619, 1727, 1736
NarI GGCGCC 1 cut(s) 1763
NciI CCSGG 3 cut(s) 939, 1079, 1582
NcoI CCATGG 3 cut(s) 511, 915, 968
NdeI CATATG 2 cut(s) 1438, 1624
NdeII GATC 6 cut(s) 208, 569, 862, 1473, 1786, 1994
NmuCI GTSAC 4 cut(s) 952, 1650, 1981, 2107
NspI RCATGY 1 cut(s) 1599
PaqCI CACCTGC 1 cut(s) 1121
PceI AGGCCT 1 cut(s) 837
PciSI GCTCTTC 1 cut(s) 12
PctI GAATGC 1 cut(s) 782
PfeI GAWTC 1 cut(s) 2056
PflMI CCANNNNNTGG 3 cut(s) 489, 1547, 1772
PfoI TCCNGGA 2 cut(s) 937, 1510
PleI GAGTC 5 cut(s) 451, 596, 598, 1047, 2034
PluTI GGCGCC 1 cut(s) 1766
PpsI GAGTC 5 cut(s) 451, 596, 598, 1047, 2034
Ppu21I YACGTR 1 cut(s) 716
PshBI ATTAAT 1 cut(s) 1893
Psp6I CCWGG 4 cut(s) 1151, 1510, 1771, 1859
PspEI GGTNACC 1 cut(s) 1650
PspFI CCCAGC 3 cut(s) 641, 682, 1232
PspGI CCWGG 4 cut(s) 1151, 1510, 1771, 1859
PspOMI GGGCCC 2 cut(s) 131, 1462
PstNI CAGNNNCTG 1 cut(s) 641
PsuI RGATCY 1 cut(s) 208
PvuII CAGCTG 2 cut(s) 641, 1262
RsaI GTAC 3 cut(s) 705, 728, 1784
RsaNI GTAC 3 cut(s) 704, 727, 1783
RseI CAYNNNNRTG 1 cut(s) 416
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 5 cut(s) 858, 1097, 1191, 1893, 2215
Sau3AI GATC 6 cut(s) 208, 569, 862, 1473, 1786, 1994
SchI GAGTC 5 cut(s) 452, 596, 599, 1047, 2034
ScrFI CCNGG 7 cut(s) 939, 1079, 1153, 1512, 1582, 1773, 1861
SduI GDGCHC 5 cut(s) 135, 550, 1446, 1466, 2192
SfaNI GCATC 8 cut(s) 73, 638, 754, 759, 781, 1102, 1159, 1334
SfcI CTRYAG 1 cut(s) 1608
SfoI GGCGCC 1 cut(s) 1764
SinI GGWCC 2 cut(s) 473, 935
SmiMI CAYNNNNRTG 1 cut(s) 416
SmlI CTYRAG 2 cut(s) 604, 1490
SmoI CTYRAG 2 cut(s) 604, 1490
Sse9I AATT 9 cut(s) 432, 873, 1025, 1085, 1098, 1200, 1266, 1724, 1952
SseBI AGGCCT 1 cut(s) 837
SsiI CCGC 6 cut(s) 609, 627, 779, 921, 1003, 1505
SspDI GGCGCC 1 cut(s) 1762
SspI AATATT 1 cut(s) 43
SspMI CTAG 6 cut(s) 776, 1563, 1574, 1710, 1872, 2141
StuI AGGCCT 1 cut(s) 837
StyD4I CCNGG 7 cut(s) 937, 1077, 1151, 1510, 1580, 1771, 1859
StyI CCWWGG 3 cut(s) 511, 915, 968
TaiI ACGT 3 cut(s) 174, 718, 1335
TaqI TCGA 5 cut(s) 10, 568, 786, 1224, 1457
TasI AATT 9 cut(s) 432, 873, 1025, 1085, 1098, 1200, 1266, 1724, 1952
TatI WGTACW 1 cut(s) 726
TauI GCSGC 3 cut(s) 782, 923, 1508
TfiI GAWTC 1 cut(s) 2056
Tru1I TTAA 5 cut(s) 858, 1097, 1191, 1893, 2215
Tru9I TTAA 5 cut(s) 858, 1097, 1191, 1893, 2215
TscAI CASTG 2 cut(s) 433, 657
TseFI GTSAC 4 cut(s) 952, 1650, 1981, 2107
TseI GCWGC 9 cut(s) 251, 638, 744, 747, 986, 1259, 1529, 1619, 1658
Tsp45I GTSAC 4 cut(s) 952, 1650, 1981, 2107
TspDTI ATGAA 6 cut(s) 140, 219, 1000, 1311, 1809, 1928
TspGWI ACGGA 2 cut(s) 817, 1749
TspRI CASTG 2 cut(s) 433, 657
Van91I CCANNNNNTGG 3 cut(s) 489, 1547, 1772
VpaK11BI GGWCC 2 cut(s) 473, 935
VspI ATTAAT 1 cut(s) 1893
XapI RAATTY 4 cut(s) 432, 1085, 1724, 1952
XbaI TCTAGA 1 cut(s) 1573
XceI RCATGY 1 cut(s) 1599
XcmI CCANNNNNNNNNTGG 1 cut(s) 486
XspI CTAG 6 cut(s) 776, 1563, 1574, 1710, 1872, 2141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.