Rw1G031470

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000390
Physical Location & Seq
Reverse (-)
59583771 .. 59587850
4080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G031470.1

Sequence Viewer

Length: 2088 bp
ATGGGAAACAAAAGGTTGACAAGCCCAAACCTACATTGTATATACATGCATCTCCTATCGTTCAAGGCTTATATTGTCTATATGGGCGACAAGCCAAAGAACGGGATGCCAGTAACACCTGATCTTCATGATATTGCACATGAAGCTCTCCTTCTTCACAGCTACAAGAGAAGTTTCCATGGATTTGCTGCAATGCTCACAGAACAAGAAGCACAAAAACTGGCTGGAATGGATGGTGTAGTGTCTGTCTTTCCAAGTAAAAAGGTGAGCGTCAAAACAACAACGTCATGGAATTTCGTTGGGTTTCCAGAATCAATTGGGAGCGGCCCCTTTGAAAGTGATATCATTATCGGTGTCTTTAATGATGTTGGTTTTGGTCCTCCACCTAAGAAATGGAAAGGCATATGTCAAGGCAATAACAACTTCACTTGTAACAATAAAATCATCGGAGCACGGTATTACAAAGATAGTGCCTTCGAAGAAGAAGATATTCAGTCTCCAAGAGACTCATCCGGTCATGGAACCCACTGCGCGTCAATAGCAGCTGGGAATGCAGTGAGCAACGCAAGCATAAATGCACGTATTGCGGTGTACAAAATATGTTGGGACAAAGGTTGTGGTGTTGCTGAGTTTCTAGCGGCAATCGATGATGCCATTGCTGACGGTGTCGACATTATCAGTGCTTCCACCGGAGGCGCTGCGCCAGCTGACTTTTTGAATGACGGATTTGCAATTGGGGCATTTCACGCTACCGTAAATGGGATATTGGTTTCTATGGCCGCTGGTAATGAAGGTCCGCAAAAAAAAACTGTGGAAAACATTGCACCATGGCAACTTTCTGTGGCTGCTACCACCACAAACCGCCAGTTCATCACCAAGGTTAAATTGGGTAATGGTAAAATCTATGAGGGACTTTCCGTACATATACATGACCTCCAAGGTAAATTTTACCCGTTGATTTACGGTGGAGACGCACCCAGTAAAGAAGATGGTAAAGGAGATGATTCTAGGTTTTGTGTTGGAGATAGCTTAGATGCAGATAAGATCAAAGGTAAAATTGTGCTTTGTGATCGCCCCGATAAGGATGGGAAGGGGGCTGGGGATGGTGCCAAATGGTGGAATGCTTCAATGGGAGTTATTCTGACGGGCAAACAAGTTGTCGAGGAAACCGAATATGCTGATAACCCTTGTGCAGCATCTTACGTCAATTTAGAAGATAATAGCAACATTTACAAATACATAAACAAAACAAGGGACCCAACTGCAACTATTTTGAAAAGTGAGGAGATCAATGATGTATTGGCTCCATACGTGCCCTCCTACTCATCAAGGGGTCCAAACGCGTTGAATCCCAACATACTGAAGCCAGATTTAGCGGCTCCGGGAACTTACATTCTAGCAGCATATCCCCCAAATGTTAATAATGGAGCTAAATACAAATTGGCAACCGGCACATCAATGGCATGCCCCCATGCTTCGTCTGTAGCTGCGTACGTCAAATCATTTCACCCTAAATGGTCACCGGCTGCTGTCCAATCAGCTCTCATCACTACTGCTACGCCTATGAGTGACAAAACTACCCCGCACGCTGAAATTGCATATGGAGCTGGCCTAATAAACCCTTCCAGAGCTCCATATCCTGGTTTGGTTTACGATCTTGATGTACAAGACTATGTAGATTTTTTGTGTTCACAAGGATATAGTGACAAACTATTAGAACAAATAACCAGGGACAAGACTACCTCATGCTCATCAAAATTTGATAATGAAACAGTCAATGACCTGAATTATCCTTCTTTTGCTCTTCCTATCAAGGATCCGAAATTCGTCAATGGGGTTTTCCATAGGACTGTCACTAATGTCGGATCATCAAATTCTATATACAGAGTTAAAGTGGTGGCTCCATCGGGACTCAAAATCAATGTGGATCCAAGTGTGTTGTCGTTCACATCTCTTGAGCAAAAGAAATCTTTTGTAGTCACCGTAAAAGGGCCGATTGAGAAAACAAGTATGGTCTCTGCGTCTTTGGTTTGGGACGATGGTGCTTTCCAAGTCAGGAGCCCCATTGTTGTCTATATTCAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

695

Amino Acids

74.79

Weight (kDa)

6.44

Isoelectric Point (pI)

33.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 24 - 91 1.3e-12 Peptidase inhibitor I9
Peptidase_S8 PF00082 153 - 537 1.2e-39 Subtilase family
fn3_6 PF17766 594 - 691 3.3e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1106
AccB7I CCANNNNNTGG 2 cut(s) 1116, 1640
AccBSI CCGCTC 1 cut(s) 324
AccI GTMKAC 1 cut(s) 669
AccII CGCG 2 cut(s) 533, 1343
AciI CCGC 8 cut(s) 324, 587, 638, 780, 797, 862, 1376, 1583
AclWI GGATC 5 cut(s) 1811, 1824, 1873, 1922, 1935
AcoI YGGCCR 1 cut(s) 777
AcsI RAATTY 5 cut(s) 292, 944, 1757, 1823, 1873
AcuI CTGAAG 1 cut(s) 1382
AfaI GTAC 4 cut(s) 593, 921, 1493, 1665
AfiI CCNNNNNNNGG 6 cut(s) 101, 759, 1081, 1116, 1640, 1989
AflIII ACRYGT 1 cut(s) 1341
AgsI TTSAA 7 cut(s) 64, 335, 718, 1128, 1276, 1348, 2081
AjnI CCWGG 2 cut(s) 1639, 1727
Alw21I GWGCWC 2 cut(s) 454, 1633
Alw26I GTCTC 4 cut(s) 498, 501, 963, 2020
AlwI GGATC 5 cut(s) 1811, 1824, 1873, 1922, 1935
AoxI GGCC 4 cut(s) 325, 777, 1609, 1991
ApeKI GCWGC 8 cut(s) 188, 542, 698, 845, 1193, 1400, 1487, 1526
ApoI RAATTY 5 cut(s) 292, 944, 1757, 1823, 1873
ArsI GACNNNNNNTTYG 2 cut(s) 1143, 1175
Asp700I GAANNNNTTC 1 cut(s) 489
AspLEI GCGC 3 cut(s) 533, 698, 703
AspS9I GGNCC 6 cut(s) 326, 377, 794, 1255, 1334, 1991
AsuC2I CCSGG 1 cut(s) 1383
AsuHPI GGTGA 5 cut(s) 277, 865, 1499, 1512, 1972
AsuII TTCGAA 1 cut(s) 477
AvaII GGWCC 4 cut(s) 377, 794, 1255, 1334
BaeGI GKGCMC 1 cut(s) 1317
BamHI GGATCC 2 cut(s) 1816, 1927
BanI GGYRCC 1 cut(s) 1106
BanII GRGCYC 2 cut(s) 1633, 2063
Bbv12I GWGCWC 2 cut(s) 454, 1633
BbvI GCAGC 8 cut(s) 175, 554, 685, 832, 1205, 1412, 1474, 1513
BccI CCATC 6 cut(s) 227, 983, 1079, 1097, 1912, 2033
BciT130I CCWGG 2 cut(s) 1641, 1729
BcnI CCSGG 1 cut(s) 1383
BcoDI GTCTC 4 cut(s) 498, 501, 963, 2020
BfaI CTAG 3 cut(s) 635, 1008, 1397
BfmI CTRYAG 2 cut(s) 1482, 2084
BfoI RGCGCY 1 cut(s) 699
Bme1390I CCNGG 3 cut(s) 1383, 1641, 1729
Bme18I GGWCC 4 cut(s) 377, 794, 1255, 1334
BmgT120I GGNCC 6 cut(s) 326, 377, 794, 1255, 1334, 1991
BmrFI CCNGG 3 cut(s) 1383, 1641, 1729
BmrI ACTGGG 1 cut(s) 972
BmsI GCATC 5 cut(s) 58, 96, 640, 1024, 1205
BmuI ACTGGG 1 cut(s) 972
Bpu14I TTCGAA 1 cut(s) 477
BpuEI CTTGAG 1 cut(s) 1976
BpuMI CCSGG 1 cut(s) 1383
Bsa29I ATCGAT 1 cut(s) 645
BsaAI YACGTR 2 cut(s) 581, 1312
BsaI GGTCTC 1 cut(s) 2020
BsaJI CCNNGG 5 cut(s) 178, 827, 876, 937, 1728
BsaWI WCCGGW 2 cut(s) 512, 689
BsaXI ACNNNNNCTCC 4 cut(s) 918, 948, 1301, 1331
Bsc4I CCNNNNNNNGG 6 cut(s) 101, 759, 1081, 1116, 1640, 1989
Bse118I RCCGGY 2 cut(s) 1448, 1522
Bse1I ACTGG 4 cut(s) 110, 225, 865, 978
Bse3DI GCAATG 3 cut(s) 198, 654, 819
BseBI CCWGG 2 cut(s) 1641, 1729
BseCI ATCGAT 1 cut(s) 645
BseDI CCNNGG 5 cut(s) 178, 827, 876, 937, 1728
BseGI GGATG 5 cut(s) 111, 238, 509, 1090, 1108
BseLI CCNNNNNNNGG 6 cut(s) 101, 759, 1081, 1116, 1640, 1989
BseMI GCAATG 3 cut(s) 198, 654, 819
BseMII CTCAG 1 cut(s) 618
BseNI ACTGG 4 cut(s) 110, 225, 865, 978
BseRI GAGGAG 1 cut(s) 1298
BseSI GKGCMC 1 cut(s) 1317
BseXI GCAGC 8 cut(s) 175, 554, 685, 832, 1205, 1412, 1474, 1513
BseYI CCCAGC 2 cut(s) 545, 1097
BsgI GTGCAG 1 cut(s) 1212
Bsh1236I CGCG 2 cut(s) 533, 1343
BshFI GGCC 4 cut(s) 327, 779, 1611, 1993
BshNI GGYRCC 1 cut(s) 1106
BshVI ATCGAT 1 cut(s) 645
BsiHKAI GWGCWC 2 cut(s) 454, 1633
BsiSI CCGG 5 cut(s) 513, 690, 1382, 1449, 1523
BsiWI CGTACG 1 cut(s) 1491
BslFI GGGAC 6 cut(s) 620, 924, 1268, 1745, 1923, 2048
BslI CCNNNNNNNGG 6 cut(s) 101, 759, 1081, 1116, 1640, 1989
BsmAI GTCTC 4 cut(s) 498, 501, 963, 2020
BsmBI CGTCTC 1 cut(s) 963
BsmFI GGGAC 6 cut(s) 620, 924, 1268, 1745, 1923, 2048
BsmI GAATGC 2 cut(s) 556, 1126
BsnI GGCC 4 cut(s) 327, 779, 1611, 1993
Bso31I GGTCTC 1 cut(s) 2020
Bsp119I TTCGAA 1 cut(s) 477
Bsp1286I GDGCHC 4 cut(s) 454, 1317, 1633, 2063
Bsp1407I TGTACA 2 cut(s) 591, 1663
Bsp143I GATC 8 cut(s) 121, 1044, 1069, 1287, 1654, 1816, 1865, 1927
Bsp19I CCATGG 2 cut(s) 178, 827
BspACI CCGC 8 cut(s) 324, 587, 638, 780, 797, 862, 1376, 1583
BspANI GGCC 4 cut(s) 327, 779, 1611, 1993
BspCNI CTCAG 1 cut(s) 619
BspDI ATCGAT 1 cut(s) 645
BspFNI CGCG 2 cut(s) 533, 1343
BspHI TCATGA 1 cut(s) 127
BspPI GGATC 5 cut(s) 1811, 1824, 1873, 1922, 1935
BspQI GCTCTTC 1 cut(s) 1809
BspT104I TTCGAA 1 cut(s) 477
BspT107I GGYRCC 1 cut(s) 1106
BspTNI GGTCTC 1 cut(s) 2020
BsrBI CCGCTC 1 cut(s) 324
BsrDI GCAATG 3 cut(s) 198, 654, 819
BsrFI RCCGGY 2 cut(s) 1448, 1522
BsrGI TGTACA 2 cut(s) 591, 1663
BsrI ACTGG 4 cut(s) 110, 225, 865, 978
BssAI RCCGGY 2 cut(s) 1448, 1522
BssECI CCNNGG 5 cut(s) 178, 827, 876, 937, 1728
BssMI GATC 8 cut(s) 121, 1044, 1069, 1287, 1654, 1816, 1865, 1927
BssT1I CCWWGG 4 cut(s) 178, 827, 876, 937
Bst2UI CCWGG 2 cut(s) 1641, 1729
Bst4CI ACNGT 8 cut(s) 456, 665, 754, 811, 965, 1774, 1852, 1984
Bst6I CTCTTC 1 cut(s) 1809
BstAPI GCANNNNNTGC 1 cut(s) 584
BstAUI TGTACA 2 cut(s) 591, 1663
BstBAI YACGTR 2 cut(s) 581, 1312
BstBI TTCGAA 1 cut(s) 477
BstC8I GCNNGC 5 cut(s) 568, 705, 1465, 1587, 1609
BstDEI CTNAG 3 cut(s) 387, 627, 1030
BstDSI CCRYGG 2 cut(s) 178, 827
BstEII GGTNACC 1 cut(s) 1518
BstF5I GGATG 5 cut(s) 111, 238, 509, 1090, 1108
BstFNI CGCG 2 cut(s) 533, 1343
BstH2I RGCGCY 1 cut(s) 699
BstHHI GCGC 3 cut(s) 533, 698, 703
BstKTI GATC 8 cut(s) 124, 1047, 1072, 1290, 1657, 1819, 1868, 1930
BstMAI GTCTC 4 cut(s) 498, 501, 963, 2020
BstMBI GATC 8 cut(s) 121, 1044, 1069, 1287, 1654, 1816, 1865, 1927
BstNI CCWGG 2 cut(s) 1641, 1729
BstNSI RCATGY 2 cut(s) 49, 1467
BstPI GGTNACC 1 cut(s) 1518
BstSCI CCNGG 3 cut(s) 1381, 1639, 1727
BstSFI CTRYAG 2 cut(s) 1482, 2084
BstSLI GKGCMC 1 cut(s) 1317
BstUI CGCG 2 cut(s) 533, 1343
BstV1I GCAGC 8 cut(s) 175, 554, 685, 832, 1205, 1412, 1474, 1513
BstX2I RGATCY 2 cut(s) 1816, 1927
BstYI RGATCY 2 cut(s) 1816, 1927
Bsu15I ATCGAT 1 cut(s) 645
BsuRI GGCC 4 cut(s) 327, 779, 1611, 1993
BsuTUI ATCGAT 1 cut(s) 645
BtgI CCRYGG 2 cut(s) 178, 827
BtsCI GGATG 5 cut(s) 111, 238, 509, 1090, 1108
BtsI GCAGTG 2 cut(s) 526, 561
BtsIMutI CAGTG 3 cut(s) 526, 561, 685
Cac8I GCNNGC 5 cut(s) 568, 705, 1465, 1587, 1609
CciI TCATGA 1 cut(s) 127
CfoI GCGC 3 cut(s) 533, 698, 703
Cfr10I RCCGGY 2 cut(s) 1448, 1522
Cfr13I GGNCC 6 cut(s) 326, 377, 794, 1255, 1334, 1991
ClaI ATCGAT 1 cut(s) 645
CseI GACGC 4 cut(s) 259, 522, 980, 2010
Csp6I GTAC 4 cut(s) 592, 920, 1492, 1664
CspCI CAANNNNNGTGG 2 cut(s) 598, 633
CviQI GTAC 4 cut(s) 592, 920, 1492, 1664
DdeI CTNAG 3 cut(s) 387, 627, 1030
DpnI GATC 8 cut(s) 123, 1046, 1071, 1289, 1656, 1818, 1867, 1929
DpnII GATC 8 cut(s) 121, 1044, 1069, 1287, 1654, 1816, 1865, 1927
EaeI YGGCCR 1 cut(s) 777
Eam1104I CTCTTC 1 cut(s) 1809
EarI CTCTTC 1 cut(s) 1809
Ecl136II GAGCTC 1 cut(s) 1631
Eco130I CCWWGG 4 cut(s) 178, 827, 876, 937
Eco24I GRGCYC 2 cut(s) 1633, 2063
Eco31I GGTCTC 1 cut(s) 2020
Eco32I GATATC 1 cut(s) 343
Eco47I GGWCC 4 cut(s) 377, 794, 1255, 1334
Eco53kI GAGCTC 1 cut(s) 1631
Eco57I CTGAAG 1 cut(s) 1382
Eco91I GGTNACC 1 cut(s) 1518
EcoICRI GAGCTC 1 cut(s) 1631
EcoO109I RGGNCCY 1 cut(s) 1255
EcoO65I GGTNACC 1 cut(s) 1518
EcoRII CCWGG 2 cut(s) 1639, 1727
EcoRV GATATC 1 cut(s) 343
EcoT14I CCWWGG 4 cut(s) 178, 827, 876, 937
EcoT22I ATGCAT 1 cut(s) 51
EcoT38I GRGCYC 2 cut(s) 1633, 2063
ErhI CCWWGG 4 cut(s) 178, 827, 876, 937
Esp3I CGTCTC 1 cut(s) 963
FalI AAGNNNNNCTT 2 cut(s) 135, 167
FaqI GGGAC 6 cut(s) 620, 924, 1268, 1745, 1923, 2048
FauI CCCGC 1 cut(s) 1590
FauNDI CATATG 2 cut(s) 404, 1600
FblI GTMKAC 1 cut(s) 669
FokI GGATG 5 cut(s) 118, 245, 496, 1097, 1115
FriOI GRGCYC 2 cut(s) 1633, 2063
FspBI CTAG 3 cut(s) 635, 1008, 1397
GlaI GCGC 3 cut(s) 532, 697, 702
GsaI CCCAGC 2 cut(s) 549, 1101
HaeII RGCGCY 1 cut(s) 699
HaeIII GGCC 4 cut(s) 327, 779, 1611, 1993
HapII CCGG 5 cut(s) 513, 690, 1382, 1449, 1523
HgaI GACGC 4 cut(s) 259, 522, 980, 2010
HhaI GCGC 3 cut(s) 533, 698, 703
Hin6I GCGC 3 cut(s) 531, 696, 701
HinP1I GCGC 3 cut(s) 531, 696, 701
HincII GTYRAC 2 cut(s) 18, 670
HindII GTYRAC 2 cut(s) 18, 670
HinfI GANTC 5 cut(s) 311, 506, 1004, 1348, 1911
HpaII CCGG 5 cut(s) 513, 690, 1382, 1449, 1523
HphI GGTGA 5 cut(s) 277, 865, 1499, 1512, 1972
Hpy166II GTNNAC 6 cut(s) 18, 592, 670, 1651, 1691, 1947
Hpy188I TCNGA 4 cut(s) 449, 1143, 1821, 1865
Hpy188III TCNNGA 7 cut(s) 128, 308, 1626, 1658, 1908, 1955, 2056
Hpy8I GTNNAC 6 cut(s) 18, 592, 670, 1651, 1691, 1947
HpyAV CCTTC 6 cut(s) 161, 484, 785, 1084, 1632, 1803
HpyCH4III ACNGT 8 cut(s) 456, 665, 754, 811, 965, 1774, 1852, 1984
HpyCH4IV ACGT 5 cut(s) 284, 580, 1203, 1311, 1494
HpyF3I CTNAG 3 cut(s) 387, 627, 1030
HpySE526I ACGT 5 cut(s) 284, 580, 1203, 1311, 1494
HspAI GCGC 3 cut(s) 531, 696, 701
KflI GGGWCCC 1 cut(s) 1255
Kzo9I GATC 8 cut(s) 121, 1044, 1069, 1287, 1654, 1816, 1865, 1927
LguI GCTCTTC 1 cut(s) 1809
LmnI GCTCC 9 cut(s) 321, 449, 1309, 1384, 1427, 1604, 1636, 1906, 2058
Lsp1109I GCAGC 8 cut(s) 175, 554, 685, 832, 1205, 1412, 1474, 1513
LweI GCATC 5 cut(s) 58, 96, 640, 1024, 1205
MaeI CTAG 3 cut(s) 635, 1008, 1397
MaeII ACGT 5 cut(s) 284, 580, 1203, 1311, 1494
MaeIII GTNAC 7 cut(s) 112, 431, 1518, 1568, 1703, 1852, 1978
MalI GATC 8 cut(s) 123, 1046, 1071, 1289, 1656, 1818, 1867, 1929
MbiI CCGCTC 1 cut(s) 324
MboI GATC 8 cut(s) 121, 1044, 1069, 1287, 1654, 1816, 1865, 1927
MboII GAAGA 8 cut(s) 116, 146, 491, 494, 497, 998, 1226, 1796
MfeI CAATTG 2 cut(s) 315, 732
MflI RGATCY 2 cut(s) 1816, 1927
MhlI GDGCHC 4 cut(s) 454, 1317, 1633, 2063
MluI ACGCGT 1 cut(s) 1341
MlyI GAGTC 2 cut(s) 500, 1905
MmeI TCCRAC 2 cut(s) 1000, 1843
MnlI CCTC 8 cut(s) 390, 686, 901, 944, 1156, 1276, 1327, 1753
Mph1103I ATGCAT 1 cut(s) 51
MroXI GAANNNNTTC 1 cut(s) 489
MseI TTAA 4 cut(s) 360, 882, 1419, 1890
MslI CAYNNNNRTG 2 cut(s) 927, 1457
MspA1I CMGCKG 3 cut(s) 545, 707, 782
MspI CCGG 5 cut(s) 513, 690, 1382, 1449, 1523
MspR9I CCNGG 3 cut(s) 1383, 1641, 1729
MteI GCGCNGCGC 1 cut(s) 699
MunI CAATTG 2 cut(s) 315, 732
Mva1269I GAATGC 2 cut(s) 556, 1126
MvaI CCWGG 2 cut(s) 1641, 1729
MvnI CGCG 2 cut(s) 533, 1343
NciI CCSGG 1 cut(s) 1383
NcoI CCATGG 2 cut(s) 178, 827
NdeI CATATG 2 cut(s) 404, 1600
NdeII GATC 8 cut(s) 121, 1044, 1069, 1287, 1654, 1816, 1865, 1927
NmuCI GTSAC 5 cut(s) 1518, 1568, 1703, 1852, 1978
NsiI ATGCAT 1 cut(s) 51
NspI RCATGY 2 cut(s) 49, 1467
NspV TTCGAA 1 cut(s) 477
PaeI GCATGC 1 cut(s) 1467
PagI TCATGA 1 cut(s) 127
PciSI GCTCTTC 1 cut(s) 1809
PcsI WCGNNNNNNNCGW 1 cut(s) 1167
PctI GAATGC 2 cut(s) 556, 1126
PdmI GAANNNNTTC 1 cut(s) 489
PfeI GAWTC 3 cut(s) 311, 1004, 1348
Pfl23II CGTACG 1 cut(s) 1491
PflFI GACNNNGTC 1 cut(s) 665
PflMI CCANNNNNTGG 2 cut(s) 1116, 1640
PfoI TCCNGGA 1 cut(s) 1381
PleI GAGTC 2 cut(s) 500, 1905
PpsI GAGTC 2 cut(s) 500, 1905
Ppu21I YACGTR 2 cut(s) 581, 1312
PpuMI RGGWCCY 1 cut(s) 1255
Psp124BI GAGCTC 1 cut(s) 1633
Psp5II RGGWCCY 1 cut(s) 1255
Psp6I CCWGG 2 cut(s) 1639, 1727
PspEI GGTNACC 1 cut(s) 1518
PspFI CCCAGC 2 cut(s) 545, 1097
PspGI CCWGG 2 cut(s) 1639, 1727
PspLI CGTACG 1 cut(s) 1491
PspPI GGNCC 6 cut(s) 326, 377, 794, 1255, 1334, 1991
PspPPI RGGWCCY 1 cut(s) 1255
PsuI RGATCY 2 cut(s) 1816, 1927
PsyI GACNNNGTC 1 cut(s) 665
PvuII CAGCTG 2 cut(s) 545, 707
RsaI GTAC 4 cut(s) 593, 921, 1493, 1665
RsaNI GTAC 4 cut(s) 592, 920, 1492, 1664
RseI CAYNNNNRTG 2 cut(s) 927, 1457
SacI GAGCTC 1 cut(s) 1633
SalI GTCGAC 1 cut(s) 668
SapI GCTCTTC 1 cut(s) 1809
SaqAI TTAA 4 cut(s) 360, 882, 1419, 1890
Sau3AI GATC 8 cut(s) 121, 1044, 1069, 1287, 1654, 1816, 1865, 1927
Sau96I GGNCC 6 cut(s) 326, 377, 794, 1255, 1334, 1991
SchI GAGTC 2 cut(s) 500, 1905
ScrFI CCNGG 3 cut(s) 1383, 1641, 1729
SduI GDGCHC 4 cut(s) 454, 1317, 1633, 2063
SfaNI GCATC 5 cut(s) 58, 96, 640, 1024, 1205
SfcI CTRYAG 2 cut(s) 1482, 2084
SfuI TTCGAA 1 cut(s) 477
SinI GGWCC 4 cut(s) 377, 794, 1255, 1334
SmiMI CAYNNNNRTG 2 cut(s) 927, 1457
SmlI CTYRAG 1 cut(s) 1955
SmoI CTYRAG 1 cut(s) 1955
SphI GCATGC 1 cut(s) 1467
SsiI CCGC 8 cut(s) 324, 587, 638, 780, 797, 862, 1376, 1583
SspMI CTAG 3 cut(s) 635, 1008, 1397
SstI GAGCTC 1 cut(s) 1633
StyD4I CCNGG 3 cut(s) 1381, 1639, 1727
StyI CCWWGG 4 cut(s) 178, 827, 876, 937
TaaI ACNGT 8 cut(s) 456, 665, 754, 811, 965, 1774, 1852, 1984
TaiI ACGT 5 cut(s) 287, 583, 1206, 1314, 1497
TaqI TCGA 4 cut(s) 477, 645, 669, 1161
TatI WGTACW 2 cut(s) 591, 1663
TauI GCSGC 4 cut(s) 327, 641, 782, 1379
TfiI GAWTC 3 cut(s) 311, 1004, 1348
Tru1I TTAA 4 cut(s) 360, 882, 1419, 1890
Tru9I TTAA 4 cut(s) 360, 882, 1419, 1890
TscAI CASTG 3 cut(s) 533, 561, 685
TseFI GTSAC 5 cut(s) 1518, 1568, 1703, 1852, 1978
TseI GCWGC 8 cut(s) 188, 542, 698, 845, 1193, 1400, 1487, 1526
Tsp45I GTSAC 5 cut(s) 1518, 1568, 1703, 1852, 1978
TspDTI ATGAA 5 cut(s) 116, 156, 804, 859, 1782
TspGWI ACGGA 2 cut(s) 738, 907
TspRI CASTG 3 cut(s) 533, 561, 685
Tth111I GACNNNGTC 1 cut(s) 665
Van91I CCANNNNNTGG 2 cut(s) 1116, 1640
VpaK11BI GGWCC 4 cut(s) 377, 794, 1255, 1334
XapI RAATTY 5 cut(s) 292, 944, 1757, 1823, 1873
XceI RCATGY 2 cut(s) 49, 1467
XcmI CCANNNNNNNNNTGG 2 cut(s) 390, 883
XmiI GTMKAC 1 cut(s) 669
XmnI GAANNNNTTC 1 cut(s) 489
XspI CTAG 3 cut(s) 635, 1008, 1397
Zsp2I ATGCAT 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.