Rw1G005870

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000390
Physical Location & Seq
Reverse (-)
11875680 .. 11880153
4474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G005870.1

Sequence Viewer

Length: 2211 bp
ATGGCTCTTCGATGGTTTCTCCTTCTCAACCTCATTTGCGGTATTCTACTGCTTGTCGATGTTACTCAATCAACAGTTCAAGATGCCCGAAAGGCTTATGTTGTGTATATGGGCGACAAGCCAAAGAATGGGGTATCCATATTACCTGATCTTCATGTGAATATGCTACAGGACGTAGTTGACAACAGCAACATTGATATTGCACATGAAGCGCTTCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATTACCGGAATGGATGGTGTAGTGTCTGTCTTCCCAAGCAAACAAAGGAGCGTCAAAACAACAAAGTCATGGAACTTCCTTGGGTTTCCAGAAACAGTTAAGCGAAGCGCCCTTGAAAGTAATATCATTGTCGGTGTGATAGACACTGGAATTTGGCCAGAGTCGGACAGCTTTAGTGATGCTGGTTTTGGTCCTCCACCTGAGAAATGGAAAGGCAAATGTCAAGGCAATGGAAATCTTACTTGTAACAATAAAATCATCGGAGCACAGTATTACCGTGCTTCTCGATCCTTCGAAAGAGATGTTGAGTCTCCAAGAGACTCAAATGGTCATGGAACGCATACTGCATCAACAGCAGCTGGGAACACAGTGAGCAAGGCAAGCCTAAATGGTTTAGGGTTGGGAACAGCAAGAGGCGGGGTGCCATCAGCACGTATTGCCGTGTACAAAGTATGCTGGAGCAGCAGGCCTGCTTGTGAAGATGCTGACATTTTAGCGGCATTCGATGATGCCATTGCTGACGGTGTCGACATTCTCTCTATTTCTATCGGGAGCGATATTCCAGTTGATTATTTGACTGATTCATTGGCAATTGGGTCATTTCACGCTACCAGAAATGGGATATTAGTTTCCCTGGTCGCTGGTAACGAAGGTCCGGGAGCAAAAACTGTGACAAACTTTGCACCATGGCAACTTTCTGTGGCTGCTTCCACCATAAACCGCCAATTCATCACCAAGGTTCAATTGGGTAACGGTAAAATCTATGAGGGACTTTTACCAAACATATATGACCTTCGGGGTAAATTCTATCCTTTAATTTATGGTGGTGACGCACCCAACAAAAAAGTAGGTGCTAAAGGGACCGACTCCAGGTTTTGTCTCATAGATACCTTAGAAGAAGATTTGATCAAAGATAAAATTGTGCTTTGTGATGGCCCTGTTGGGGATGGAGCCATATTGGATGGTGCAGCAGGAATTGTTCTAACAGGACAAAAAGTTGCCGATGAAGTGCTTGACCCTCTTCCCGTGCCTGCATCAAACGTCGGGTTGGAAGAAAATAGCAACATTTACAAATACATAAACTTAACAAGGAACGCAACTGCAACTATTTGGAAAAGTGAAGAGATTAATGATGTATTGGCTCCATACGTGCCCTCATACTCATCAAGGGGTCCAAATCCGATCAATCCCAACATTCTCAAGCCAGATTTAGCGGCTCCAGGAACTTACATTCTAGCAGCATATCCCCCAAGTGGTGGTGGCAGTAGAGCTAGATATTATTTAGAAACTGGGACATCAATGGCGTGCCCCCATGCTACAGCCGTAGCTGCATACGTCAAATCATTTCACCCTAAATGGTCACCGGCTGCTATCCAATCGGCTCTCATCACTACCGCTAAATCTATGAGTGCCAAAACTAGCCCTGGTGCTGAATTTGCATACGGAGCTGGCCTGATAAACCCTTCTAAGGCTCCATATCCTGGTTTGGTGTACGATCTTGATGAACAAGACTACATAGATTTTTTGTGTTCACAAGGATATAGTGGCAAACTATTGCAAGCCATAACCAGGGACAACACTAGTTGTTCATCAAAATCTAATAATGGAACAACCAATGATCTGAACTATCCTTCTTTTGCACTTTCCATCAAGGATCCGAAACTTGCCAATGGAGTCTTCCATAGGACTGTTACTAATGTTGGATCATCGAATTCCACATACAGAGTTAAAGTGGTGGCTCCTTTAGGACTCAAAATCAATGTGAATCCAAGTGTGCTATCATTCACATCTCTCGGGCAAAAGAAATCTTTTGTTGTCACTATAAACGGGCTGATTGAGAAATCAAATTTAGTCTCTGCATCTTTGGTGTGGGATGATGGTGTTTTCCAAGTCAGGAGCCCCATCGTTGTCTATGTTGCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

736

Amino Acids

78.62

Weight (kDa)

6.76

Isoelectric Point (pI)

35.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 112 3.4e-14 Peptidase inhibitor I9
Peptidase_S8 PF00082 135 - 579 4.9e-44 Subtilase family
fn3_6 PF17766 635 - 732 6.7e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 706
AccB7I CCANNNNNTGG 3 cut(s) 128, 1541, 1766
AccI GTMKAC 1 cut(s) 813
AciI CCGC 6 cut(s) 39, 702, 782, 1006, 1499, 1680
AclWI GGATC 4 cut(s) 567, 1934, 1947, 1996
AcoI YGGCCR 1 cut(s) 440
AcsI RAATTY 5 cut(s) 435, 1088, 1718, 1996, 2131
AfaI GTAC 2 cut(s) 731, 1778
AfeI AGCGCT 1 cut(s) 213
AfiI CCNNNNNNNGG 9 cut(s) 128, 903, 1155, 1228, 1538, 1541, 1753, 1766, 1854
AgsI TTSAA 4 cut(s) 80, 244, 401, 1028
AhlI ACTAGT 1 cut(s) 1865
AjnI CCWGG 6 cut(s) 918, 1154, 1504, 1708, 1765, 1853
AjuI GAANNNNNNNTTGG 2 cut(s) 1946, 1978
AluBI AGCT 6 cut(s) 228, 456, 644, 1556, 1613, 1733
AluI AGCT 6 cut(s) 228, 456, 644, 1556, 1613, 1733
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 4 cut(s) 597, 600, 1169, 2143
AlwI GGATC 4 cut(s) 567, 1934, 1947, 1996
AlwNI CAGNNNCTG 1 cut(s) 644
Ama87I CYCGRG 1 cut(s) 2078
Aor51HI AGCGCT 1 cut(s) 213
AoxI GGCC 4 cut(s) 440, 752, 1219, 1735
ApeKI GCWGC 8 cut(s) 254, 641, 747, 989, 1253, 1523, 1613, 1652
ApoI RAATTY 5 cut(s) 435, 1088, 1718, 1996, 2131
AseI ATTAAT 1 cut(s) 1413
Asp700I GAANNNNTTC 1 cut(s) 213
AspLEI GCGC 2 cut(s) 214, 395
AspS9I GGNCC 5 cut(s) 476, 938, 1146, 1220, 1457
AsuC2I CCSGG 1 cut(s) 942
AsuHPI GGTGA 4 cut(s) 1009, 1124, 1625, 1638
AsuII TTCGAA 1 cut(s) 579
AvaI CYCGRG 1 cut(s) 2078
AvaII GGWCC 4 cut(s) 476, 938, 1146, 1457
BaeGI GKGCMC 2 cut(s) 1440, 1595
BalI TGGCCA 1 cut(s) 442
BamHI GGATCC 1 cut(s) 1939
BanI GGYRCC 1 cut(s) 706
BanII GRGCYC 1 cut(s) 2186
BbsI GAAGAC 2 cut(s) 307, 1954
Bbv12I GWGCWC 1 cut(s) 553
BbvI GCAGC 8 cut(s) 241, 653, 759, 976, 1265, 1535, 1600, 1639
BccI CCATC 9 cut(s) 6, 293, 718, 1211, 1226, 1241, 1940, 2156, 2195
BceAI ACGGC 2 cut(s) 710, 1592
BciT130I CCWGG 6 cut(s) 920, 1156, 1506, 1710, 1767, 1855
BciVI GTATCC 1 cut(s) 145
BclI TGATCA 1 cut(s) 1191
BcnI CCSGG 1 cut(s) 942
BcoDI GTCTC 4 cut(s) 597, 600, 1169, 2143
BcuI ACTAGT 1 cut(s) 1865
BfaI CTAG 4 cut(s) 1520, 1557, 1704, 1866
BfmI CTRYAG 2 cut(s) 167, 1602
BfoI RGCGCY 2 cut(s) 215, 396
BfuI GTATCC 1 cut(s) 145
BglI GCCNNNNNGGC 1 cut(s) 92
Bme1390I CCNGG 7 cut(s) 920, 942, 1156, 1506, 1710, 1767, 1855
Bme18I GGWCC 4 cut(s) 476, 938, 1146, 1457
BmeT110I CYCGRG 1 cut(s) 2078
BmgT120I GGNCC 5 cut(s) 476, 938, 1146, 1220, 1457
BmrFI CCNGG 7 cut(s) 920, 942, 1156, 1506, 1710, 1767, 1855
BmrI ACTGGG 1 cut(s) 1584
BmsI GCATC 7 cut(s) 73, 454, 641, 757, 784, 1328, 2153
BmuI ACTGGG 1 cut(s) 1584
BpiI GAAGAC 2 cut(s) 307, 1954
BpmI CTGGAG 3 cut(s) 763, 1138, 1488
Bpu14I TTCGAA 1 cut(s) 579
BpuEI CTTGAG 1 cut(s) 1469
BpuMI CCSGG 1 cut(s) 942
BsaAI YACGTR 2 cut(s) 719, 1435
BsaJI CCNNGG 6 cut(s) 364, 918, 971, 1020, 1708, 1854
BsaWI WCCGGW 1 cut(s) 290
Bsc4I CCNNNNNNNGG 9 cut(s) 128, 903, 1155, 1228, 1538, 1541, 1753, 1766, 1854
Bse118I RCCGGY 1 cut(s) 1648
Bse1I ACTGG 3 cut(s) 436, 848, 1579
Bse3DI GCAATG 3 cut(s) 264, 520, 798
BseBI CCWGG 6 cut(s) 920, 1156, 1506, 1710, 1767, 1855
BseDI CCNNGG 6 cut(s) 364, 918, 971, 1020, 1708, 1854
BseGI GGATG 4 cut(s) 304, 1237, 1252, 2164
BseLI CCNNNNNNNGG 9 cut(s) 128, 903, 1155, 1228, 1538, 1541, 1753, 1766, 1854
BseMI GCAATG 3 cut(s) 264, 520, 798
BseMII CTCAG 1 cut(s) 477
BseNI ACTGG 3 cut(s) 436, 848, 1579
BseSI GKGCMC 2 cut(s) 1440, 1595
BseXI GCAGC 8 cut(s) 241, 653, 759, 976, 1265, 1535, 1600, 1639
BseYI CCCAGC 1 cut(s) 644
BsgI GTGCAG 1 cut(s) 1272
BshFI GGCC 4 cut(s) 442, 754, 1221, 1737
BshNI GGYRCC 1 cut(s) 706
BsiHKAI GWGCWC 1 cut(s) 553
BsiHKCI CYCGRG 1 cut(s) 2078
BsiSI CCGG 3 cut(s) 291, 941, 1649
BslFI GGGAC 4 cut(s) 1068, 1159, 1591, 1871
BslI CCNNNNNNNGG 9 cut(s) 128, 903, 1155, 1228, 1538, 1541, 1753, 1766, 1854
BsmAI GTCTC 4 cut(s) 597, 600, 1169, 2143
BsmFI GGGAC 4 cut(s) 1068, 1159, 1591, 1871
BsmI GAATGC 1 cut(s) 785
BsnI GGCC 4 cut(s) 442, 754, 1221, 1737
BsoBI CYCGRG 1 cut(s) 2078
Bsp119I TTCGAA 1 cut(s) 579
Bsp1286I GDGCHC 4 cut(s) 553, 1440, 1595, 2186
Bsp1407I TGTACA 1 cut(s) 729
Bsp143I GATC 8 cut(s) 148, 572, 1191, 1467, 1780, 1903, 1939, 1988
Bsp19I CCATGG 1 cut(s) 971
BspACI CCGC 6 cut(s) 39, 702, 782, 1006, 1499, 1680
BspANI GGCC 4 cut(s) 442, 754, 1221, 1737
BspCNI CTCAG 1 cut(s) 478
BspPI GGATC 4 cut(s) 567, 1934, 1947, 1996
BspQI GCTCTTC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 579
BspT107I GGYRCC 1 cut(s) 706
BsrDI GCAATG 3 cut(s) 264, 520, 798
BsrFI RCCGGY 1 cut(s) 1648
BsrGI TGTACA 1 cut(s) 729
BsrI ACTGG 3 cut(s) 436, 848, 1579
BssAI RCCGGY 1 cut(s) 1648
BssECI CCNNGG 6 cut(s) 364, 918, 971, 1020, 1708, 1854
BssMI GATC 8 cut(s) 148, 572, 1191, 1467, 1780, 1903, 1939, 1988
BssT1I CCWWGG 3 cut(s) 364, 971, 1020
Bst2UI CCWGG 6 cut(s) 920, 1156, 1506, 1710, 1767, 1855
Bst4CI ACNGT 9 cut(s) 76, 382, 555, 563, 655, 809, 955, 1040, 1975
Bst6I CTCTTC 3 cut(s) 12, 1311, 1401
BstAPI GCANNNNNTGC 1 cut(s) 722
BstAUI TGTACA 1 cut(s) 729
BstBAI YACGTR 2 cut(s) 719, 1435
BstBI TTCGAA 1 cut(s) 579
BstC8I GCNNGC 7 cut(s) 667, 752, 756, 1317, 1591, 1735, 1845
BstDEI CTNAG 3 cut(s) 486, 1177, 1752
BstDSI CCRYGG 1 cut(s) 971
BstEII GGTNACC 1 cut(s) 1644
BstF5I GGATG 4 cut(s) 304, 1237, 1252, 2164
BstH2I RGCGCY 2 cut(s) 215, 396
BstHHI GCGC 2 cut(s) 214, 395
BstKTI GATC 8 cut(s) 151, 575, 1194, 1470, 1783, 1906, 1942, 1991
BstMAI GTCTC 4 cut(s) 597, 600, 1169, 2143
BstMBI GATC 8 cut(s) 148, 572, 1191, 1467, 1780, 1903, 1939, 1988
BstNI CCWGG 6 cut(s) 920, 1156, 1506, 1710, 1767, 1855
BstPI GGTNACC 1 cut(s) 1644
BstSCI CCNGG 7 cut(s) 918, 940, 1154, 1504, 1708, 1765, 1853
BstSFI CTRYAG 2 cut(s) 167, 1602
BstSLI GKGCMC 2 cut(s) 1440, 1595
BstV1I GCAGC 8 cut(s) 241, 653, 759, 976, 1265, 1535, 1600, 1639
BstV2I GAAGAC 2 cut(s) 307, 1954
BstX2I RGATCY 1 cut(s) 1939
BstYI RGATCY 1 cut(s) 1939
BsuI GTATCC 1 cut(s) 145
BsuRI GGCC 4 cut(s) 442, 754, 1221, 1737
BtgI CCRYGG 1 cut(s) 971
BtsCI GGATG 4 cut(s) 304, 1237, 1252, 2164
BtsIMutI CAGTG 2 cut(s) 429, 660
Cac8I GCNNGC 7 cut(s) 667, 752, 756, 1317, 1591, 1735, 1845
CaiI CAGNNNCTG 1 cut(s) 644
CfoI GCGC 2 cut(s) 214, 395
Cfr10I RCCGGY 1 cut(s) 1648
Cfr13I GGNCC 5 cut(s) 476, 938, 1146, 1220, 1457
CseI GACGC 2 cut(s) 325, 1124
Csp6I GTAC 2 cut(s) 730, 1777
CviAII CATG 6 cut(s) 155, 206, 354, 617, 972, 1598
CviQI GTAC 2 cut(s) 730, 1777
DdeI CTNAG 3 cut(s) 486, 1177, 1752
DpnI GATC 8 cut(s) 150, 574, 1193, 1469, 1782, 1905, 1941, 1990
DpnII GATC 8 cut(s) 148, 572, 1191, 1467, 1780, 1903, 1939, 1988
EaeI YGGCCR 1 cut(s) 440
Eam1104I CTCTTC 3 cut(s) 12, 1311, 1401
EarI CTCTTC 3 cut(s) 12, 1311, 1401
Eco130I CCWWGG 3 cut(s) 364, 971, 1020
Eco147I AGGCCT 1 cut(s) 754
Eco24I GRGCYC 1 cut(s) 2186
Eco47I GGWCC 4 cut(s) 476, 938, 1146, 1457
Eco47III AGCGCT 1 cut(s) 213
Eco88I CYCGRG 1 cut(s) 2078
Eco91I GGTNACC 1 cut(s) 1644
EcoO65I GGTNACC 1 cut(s) 1644
EcoRI GAATTC 1 cut(s) 1996
EcoRII CCWGG 6 cut(s) 918, 1154, 1504, 1708, 1765, 1853
EcoT14I CCWWGG 3 cut(s) 364, 971, 1020
EcoT38I GRGCYC 1 cut(s) 2186
ErhI CCWWGG 3 cut(s) 364, 971, 1020
FaeI CATG 6 cut(s) 158, 209, 357, 620, 975, 1601
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FaqI GGGAC 4 cut(s) 1068, 1159, 1591, 1871
FatI CATG 6 cut(s) 154, 205, 353, 616, 971, 1597
FauI CCCGC 1 cut(s) 695
FbaI TGATCA 1 cut(s) 1191
FblI GTMKAC 1 cut(s) 813
FokI GGATG 4 cut(s) 311, 1244, 1259, 2171
FriOI GRGCYC 1 cut(s) 2186
FspBI CTAG 4 cut(s) 1520, 1557, 1704, 1866
GlaI GCGC 2 cut(s) 213, 394
GsaI CCCAGC 1 cut(s) 648
GsuI CTGGAG 3 cut(s) 763, 1138, 1488
HaeII RGCGCY 2 cut(s) 215, 396
HaeIII GGCC 4 cut(s) 442, 754, 1221, 1737
HapII CCGG 3 cut(s) 291, 941, 1649
HgaI GACGC 2 cut(s) 325, 1124
HhaI GCGC 2 cut(s) 214, 395
Hin1II CATG 6 cut(s) 158, 209, 357, 620, 975, 1601
Hin6I GCGC 2 cut(s) 212, 393
HinP1I GCGC 2 cut(s) 212, 393
HincII GTYRAC 2 cut(s) 181, 814
HindII GTYRAC 2 cut(s) 181, 814
HinfI GANTC 8 cut(s) 446, 593, 605, 866, 1151, 1959, 2034, 2050
HpaII CCGG 3 cut(s) 291, 941, 1649
HphI GGTGA 4 cut(s) 1009, 1124, 1625, 1638
Hpy166II GTNNAC 5 cut(s) 181, 730, 814, 1777, 1817
Hpy188I TCNGA 5 cut(s) 451, 548, 1467, 1908, 1944
Hpy188III TCNNGA 6 cut(s) 80, 374, 570, 835, 1784, 2179
Hpy8I GTNNAC 5 cut(s) 181, 730, 814, 1777, 1817
Hpy99I CGWCG 1 cut(s) 1331
HpyAV CCTTC 6 cut(s) 32, 586, 929, 1088, 1758, 1926
HpyCH4III ACNGT 9 cut(s) 76, 382, 555, 563, 655, 809, 955, 1040, 1975
HpyCH4IV ACGT 5 cut(s) 174, 718, 1326, 1434, 1620
HpyF3I CTNAG 3 cut(s) 486, 1177, 1752
HpySE526I ACGT 5 cut(s) 174, 718, 1326, 1434, 1620
Hsp92II CATG 6 cut(s) 158, 209, 357, 620, 975, 1601
HspAI GCGC 2 cut(s) 212, 393
Ksp22I TGATCA 1 cut(s) 1191
Kzo9I GATC 8 cut(s) 148, 572, 1191, 1467, 1780, 1903, 1939, 1988
LguI GCTCTTC 1 cut(s) 12
Lsp1109I GCAGC 8 cut(s) 241, 653, 759, 976, 1265, 1535, 1600, 1639
LweI GCATC 7 cut(s) 73, 454, 641, 757, 784, 1328, 2153
MaeI CTAG 4 cut(s) 1520, 1557, 1704, 1866
MaeII ACGT 5 cut(s) 174, 718, 1326, 1434, 1620
MaeIII GTNAC 9 cut(s) 61, 530, 929, 955, 1034, 1112, 1644, 1975, 2101
MalI GATC 8 cut(s) 150, 574, 1193, 1469, 1782, 1905, 1941, 1990
MboI GATC 8 cut(s) 148, 572, 1191, 1467, 1780, 1903, 1939, 1988
MfeI CAATTG 2 cut(s) 876, 1028
MflI RGATCY 1 cut(s) 1939
MhlI GDGCHC 4 cut(s) 553, 1440, 1595, 2186
MlsI TGGCCA 1 cut(s) 442
MluNI TGGCCA 1 cut(s) 442
MlyI GAGTC 6 cut(s) 455, 599, 602, 1145, 1968, 2028
MmeI TCCRAC 3 cut(s) 429, 1314, 1966
MnlI CCTC 6 cut(s) 41, 489, 692, 1045, 1314, 1450
Mox20I TGGCCA 1 cut(s) 442
MroXI GAANNNNTTC 1 cut(s) 213
MscI TGGCCA 1 cut(s) 442
MseI TTAA 6 cut(s) 384, 1100, 1370, 1413, 2013, 2209
Msp20I TGGCCA 1 cut(s) 442
MspA1I CMGCKG 1 cut(s) 644
MspI CCGG 3 cut(s) 291, 941, 1649
MspR9I CCNGG 7 cut(s) 920, 942, 1156, 1506, 1710, 1767, 1855
MunI CAATTG 2 cut(s) 876, 1028
Mva1269I GAATGC 1 cut(s) 785
MvaI CCWGG 6 cut(s) 920, 1156, 1506, 1710, 1767, 1855
NciI CCSGG 1 cut(s) 942
NcoI CCATGG 1 cut(s) 971
NdeII GATC 8 cut(s) 148, 572, 1191, 1467, 1780, 1903, 1939, 1988
NlaIII CATG 6 cut(s) 158, 209, 357, 620, 975, 1601
NmuCI GTSAC 4 cut(s) 955, 1112, 1644, 2101
NspV TTCGAA 1 cut(s) 579
PceI AGGCCT 1 cut(s) 754
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 930
PctI GAATGC 1 cut(s) 785
PdmI GAANNNNTTC 1 cut(s) 213
PfeI GAWTC 2 cut(s) 866, 2050
PflFI GACNNNGTC 1 cut(s) 809
PflMI CCANNNNNTGG 3 cut(s) 128, 1541, 1766
PfoI TCCNGGA 2 cut(s) 940, 1504
PleI GAGTC 6 cut(s) 454, 599, 601, 1145, 1967, 2028
PpsI GAGTC 6 cut(s) 454, 599, 601, 1145, 1967, 2028
Ppu21I YACGTR 2 cut(s) 719, 1435
PshBI ATTAAT 1 cut(s) 1413
Psp6I CCWGG 6 cut(s) 918, 1154, 1504, 1708, 1765, 1853
PspEI GGTNACC 1 cut(s) 1644
PspFI CCCAGC 1 cut(s) 644
PspGI CCWGG 6 cut(s) 918, 1154, 1504, 1708, 1765, 1853
PspPI GGNCC 5 cut(s) 476, 938, 1146, 1220, 1457
PstNI CAGNNNCTG 1 cut(s) 644
PsuI RGATCY 1 cut(s) 1939
PsyI GACNNNGTC 1 cut(s) 809
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 2 cut(s) 731, 1778
RsaNI GTAC 2 cut(s) 730, 1777
SalI GTCGAC 1 cut(s) 812
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 6 cut(s) 384, 1100, 1370, 1413, 2013, 2209
Sau3AI GATC 8 cut(s) 148, 572, 1191, 1467, 1780, 1903, 1939, 1988
Sau96I GGNCC 5 cut(s) 476, 938, 1146, 1220, 1457
SchI GAGTC 6 cut(s) 455, 599, 602, 1145, 1968, 2028
ScrFI CCNGG 7 cut(s) 920, 942, 1156, 1506, 1710, 1767, 1855
SduI GDGCHC 4 cut(s) 553, 1440, 1595, 2186
SfaNI GCATC 7 cut(s) 73, 454, 641, 757, 784, 1328, 2153
SfcI CTRYAG 2 cut(s) 167, 1602
SfuI TTCGAA 1 cut(s) 579
SinI GGWCC 4 cut(s) 476, 938, 1146, 1457
SmlI CTYRAG 1 cut(s) 1484
SmoI CTYRAG 1 cut(s) 1484
SpeI ACTAGT 1 cut(s) 1865
SseBI AGGCCT 1 cut(s) 754
SsiI CCGC 6 cut(s) 39, 702, 782, 1006, 1499, 1680
SspMI CTAG 4 cut(s) 1520, 1557, 1704, 1866
StuI AGGCCT 1 cut(s) 754
StyD4I CCNGG 7 cut(s) 918, 940, 1154, 1504, 1708, 1765, 1853
StyI CCWWGG 3 cut(s) 364, 971, 1020
TaaI ACNGT 9 cut(s) 76, 382, 555, 563, 655, 809, 955, 1040, 1975
TaiI ACGT 5 cut(s) 177, 721, 1329, 1437, 1623
TaqI TCGA 7 cut(s) 10, 57, 571, 579, 789, 813, 1994
TaqII GACCGA 1 cut(s) 1163
TatI WGTACW 1 cut(s) 729
TauI GCSGC 2 cut(s) 785, 1502
TfiI GAWTC 2 cut(s) 866, 2050
Tru1I TTAA 6 cut(s) 384, 1100, 1370, 1413, 2013, 2209
Tru9I TTAA 6 cut(s) 384, 1100, 1370, 1413, 2013, 2209
TscAI CASTG 2 cut(s) 436, 660
TseFI GTSAC 4 cut(s) 955, 1112, 1644, 2101
TseI GCWGC 8 cut(s) 254, 641, 747, 989, 1253, 1523, 1613, 1652
Tsp45I GTSAC 4 cut(s) 955, 1112, 1644, 2101
TspDTI ATGAA 7 cut(s) 143, 222, 858, 1003, 1305, 1803, 1863
TspGWI ACGGA 1 cut(s) 1743
TspRI CASTG 2 cut(s) 436, 660
Tth111I GACNNNGTC 1 cut(s) 809
Van91I CCANNNNNTGG 3 cut(s) 128, 1541, 1766
VpaK11BI GGWCC 4 cut(s) 476, 938, 1146, 1457
VspI ATTAAT 1 cut(s) 1413
XapI RAATTY 5 cut(s) 435, 1088, 1718, 1996, 2131
XcmI CCANNNNNNNNNTGG 2 cut(s) 489, 1027
XmiI GTMKAC 1 cut(s) 813
XmnI GAANNNNTTC 1 cut(s) 213
XspI CTAG 4 cut(s) 1520, 1557, 1704, 1866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.