Prupe.2G166200_v2.0.a1

Cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000390
Physical Location & Seq
Forward (+)
21517244 .. 21518214
971 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G166200.1

Sequence Viewer

Length: 546 bp
ATGGGAGCTGGCCAAATTGACCCTGTTAGGGCTCCATATCCTGGTTTGGTATATGATGCTACTGAACTGGACTACATAAAATTTTTGTGTGCACAAGGATATAATACCAATTACCCGGATCCCCTTCTAAGGAAAGAGCAGTCTTCTTTTATAGGTGAGGGGGAGTCTCCATCTCTTGTAGTTTTTCGATGTGGGACTCCTCTTGCTTTGCTTAGAGTTGTGATTTGTGGTGATGCTTCTTTGGCTAAGCTGCTCTTCAGTGATAATTTGAATTATCCTTGTTTTGCACTTTCCTCCTCGAAGCCAAACTCCATCAGTGGGGTTTTCAATAGGACAGCCACAAATGTTGGATCACCAAGGTCCACATATAAAGCTAAAGTGATCGGTGCAACGACAGGACTCGAAATCAAAGTTAATCCAAGCATTCTATCGTTCTTATCTCTAGGACAGAAGCTATCCTTTCAGGTCACGGGTGCTTCTCTGTTGTGGGATGATGGTACTTTCCAAGTCAGGAGCCCCATTGTAGTCTATGCTATATATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

182

Amino Acids

19.58

Weight (kDa)

8.31

Isoelectric Point (pI)

32.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 41
AclWI GGATC 3 cut(s) 113, 126, 358
AcoI YGGCCR 1 cut(s) 10
AcsI RAATTY 1 cut(s) 80
AcuI CTGAAG 1 cut(s) 241
AfaI GTAC 1 cut(s) 499
AfiI CCNNNNNNNGG 4 cut(s) 28, 41, 129, 318
AgsI TTSAA 2 cut(s) 271, 328
AjnI CCWGG 1 cut(s) 40
AluBI AGCT 4 cut(s) 8, 250, 374, 454
AluI AGCT 4 cut(s) 8, 250, 374, 454
Alw21I GWGCWC 1 cut(s) 94
Alw26I GTCTC 1 cut(s) 171
Alw44I GTGCAC 1 cut(s) 90
AlwI GGATC 3 cut(s) 113, 126, 358
AoxI GGCC 1 cut(s) 10
ApaLI GTGCAC 1 cut(s) 90
ApeKI GCWGC 1 cut(s) 250
ApoI RAATTY 1 cut(s) 80
AspS9I GGNCC 1 cut(s) 360
AsuC2I CCSGG 1 cut(s) 116
AsuHPI GGTGA 3 cut(s) 167, 242, 345
AvaII GGWCC 1 cut(s) 360
BaeGI GKGCMC 1 cut(s) 94
BalI TGGCCA 1 cut(s) 12
BamHI GGATCC 1 cut(s) 118
BanII GRGCYC 2 cut(s) 34, 518
BbsI GAAGAC 1 cut(s) 135
Bbv12I GWGCWC 1 cut(s) 94
BbvI GCAGC 1 cut(s) 237
BccI CCATC 3 cut(s) 178, 320, 488
BciT130I CCWGG 1 cut(s) 42
BcnI CCSGG 1 cut(s) 116
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 1 cut(s) 443
BisI GCNGC 1 cut(s) 251
BlpI GCTNAGC 1 cut(s) 246
BlsI GCNGC 1 cut(s) 252
Bme1390I CCNGG 2 cut(s) 42, 116
Bme18I GGWCC 1 cut(s) 360
BmgT120I GGNCC 1 cut(s) 360
BmiI GGNNCC 3 cut(s) 33, 120, 515
BmrFI CCNGG 2 cut(s) 42, 116
BmsI GCATC 2 cut(s) 46, 223
BpiI GAAGAC 1 cut(s) 135
Bpu1102I GCTNAGC 1 cut(s) 246
BpuMI CCSGG 1 cut(s) 116
BsaJI CCNNGG 1 cut(s) 356
Bsc4I CCNNNNNNNGG 4 cut(s) 28, 41, 129, 318
Bse1I ACTGG 1 cut(s) 72
BseBI CCWGG 1 cut(s) 42
BseDI CCNNGG 1 cut(s) 356
BseGI GGATG 1 cut(s) 496
BseLI CCNNNNNNNGG 4 cut(s) 28, 41, 129, 318
BseNI ACTGG 1 cut(s) 72
BseRI GAGGAG 2 cut(s) 189, 286
BseSI GKGCMC 1 cut(s) 94
BseXI GCAGC 1 cut(s) 237
BshFI GGCC 1 cut(s) 12
BsiHKAI GWGCWC 1 cut(s) 94
BsiSI CCGG 1 cut(s) 116
BslFI GGGAC 1 cut(s) 208
BslI CCNNNNNNNGG 4 cut(s) 28, 41, 129, 318
BsmAI GTCTC 1 cut(s) 171
BsmFI GGGAC 1 cut(s) 208
BsmI GAATGC 1 cut(s) 423
BsnI GGCC 1 cut(s) 12
Bsp1286I GDGCHC 3 cut(s) 34, 94, 518
Bsp143I GATC 3 cut(s) 118, 350, 381
Bsp1720I GCTNAGC 1 cut(s) 246
BspANI GGCC 1 cut(s) 12
BspLI GGNNCC 3 cut(s) 33, 120, 515
BspPI GGATC 3 cut(s) 113, 126, 358
BspQI GCTCTTC 1 cut(s) 260
BsrI ACTGG 1 cut(s) 72
BssECI CCNNGG 1 cut(s) 356
BssMI GATC 3 cut(s) 118, 350, 381
BssT1I CCWWGG 1 cut(s) 356
Bst2UI CCWGG 1 cut(s) 42
Bst6I CTCTTC 1 cut(s) 260
BstC8I GCNNGC 1 cut(s) 10
BstDEI CTNAG 3 cut(s) 128, 212, 246
BstF5I GGATG 1 cut(s) 496
BstKTI GATC 3 cut(s) 121, 353, 384
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 3 cut(s) 118, 350, 381
BstMWI GCNNNNNNNGC 1 cut(s) 242
BstNI CCWGG 1 cut(s) 42
BstSCI CCNGG 2 cut(s) 40, 114
BstSLI GKGCMC 1 cut(s) 94
BstV1I GCAGC 1 cut(s) 237
BstV2I GAAGAC 1 cut(s) 135
BstX2I RGATCY 1 cut(s) 118
BstYI RGATCY 1 cut(s) 118
BsuRI GGCC 1 cut(s) 12
BtsCI GGATG 1 cut(s) 496
BtsIMutI CAGTG 2 cut(s) 265, 322
Cac8I GCNNGC 1 cut(s) 10
Cfr13I GGNCC 1 cut(s) 360
Csp6I GTAC 1 cut(s) 498
CspCI CAANNNNNGTGG 2 cut(s) 328, 363
CviQI GTAC 1 cut(s) 498
DdeI CTNAG 3 cut(s) 128, 212, 246
DpnI GATC 3 cut(s) 120, 352, 383
DpnII GATC 3 cut(s) 118, 350, 381
EaeI YGGCCR 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
Eco130I CCWWGG 1 cut(s) 356
Eco24I GRGCYC 2 cut(s) 34, 518
Eco47I GGWCC 1 cut(s) 360
Eco57I CTGAAG 1 cut(s) 241
EcoRII CCWGG 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 356
EcoT38I GRGCYC 2 cut(s) 34, 518
ErhI CCWWGG 1 cut(s) 356
FalI AAGNNNNNCTT 4 cut(s) 239, 271, 443, 475
FaqI GGGAC 1 cut(s) 208
Fnu4HI GCNGC 1 cut(s) 251
FokI GGATG 1 cut(s) 503
FriOI GRGCYC 2 cut(s) 34, 518
Fsp4HI GCNGC 1 cut(s) 251
FspBI CTAG 1 cut(s) 443
GluI GCNGC 1 cut(s) 251
HaeIII GGCC 1 cut(s) 12
HapII CCGG 1 cut(s) 116
HinfI GANTC 3 cut(s) 164, 196, 399
HpaII CCGG 1 cut(s) 116
HphI GGTGA 3 cut(s) 167, 242, 345
Hpy166II GTNNAC 2 cut(s) 92, 363
Hpy188III TCNNGA 1 cut(s) 511
Hpy8I GTNNAC 2 cut(s) 92, 363
HpyAV CCTTC 1 cut(s) 134
HpyCH4V TGCA 3 cut(s) 92, 287, 389
HpyF10VI GCNNNNNNNGC 1 cut(s) 242
HpyF3I CTNAG 3 cut(s) 128, 212, 246
Kzo9I GATC 3 cut(s) 118, 350, 381
LguI GCTCTTC 1 cut(s) 260
LmnI GCTCC 3 cut(s) 5, 37, 513
LpnPI CCDG 8 cut(s) 27, 36, 53, 54, 129, 381, 449, 496
Lsp1109I GCAGC 1 cut(s) 237
LweI GCATC 2 cut(s) 46, 223
MaeI CTAG 1 cut(s) 443
MaeIII GTNAC 1 cut(s) 466
MalI GATC 3 cut(s) 120, 352, 383
MboI GATC 3 cut(s) 118, 350, 381
MboII GAAGA 2 cut(s) 135, 247
MflI RGATCY 1 cut(s) 118
MhlI GDGCHC 3 cut(s) 34, 94, 518
MlsI TGGCCA 1 cut(s) 12
MluCI AATT 5 cut(s) 15, 80, 109, 265, 271
MluNI TGGCCA 1 cut(s) 12
MlyI GAGTC 3 cut(s) 173, 190, 393
MmeI TCCRAC 1 cut(s) 328
MnlI CCTC 4 cut(s) 151, 210, 304, 307
Mox20I TGGCCA 1 cut(s) 12
MscI TGGCCA 1 cut(s) 12
MseI TTAA 2 cut(s) 414, 544
Msp20I TGGCCA 1 cut(s) 12
MspI CCGG 1 cut(s) 116
MspR9I CCNGG 2 cut(s) 42, 116
Mva1269I GAATGC 1 cut(s) 423
MvaI CCWGG 1 cut(s) 42
MwoI GCNNNNNNNGC 1 cut(s) 242
NciI CCSGG 1 cut(s) 116
NdeII GATC 3 cut(s) 118, 350, 381
NlaIV GGNNCC 3 cut(s) 33, 120, 515
NmuCI GTSAC 1 cut(s) 466
PciSI GCTCTTC 1 cut(s) 260
PctI GAATGC 1 cut(s) 423
PflMI CCANNNNNTGG 1 cut(s) 41
PkrI GCNGC 1 cut(s) 252
PleI GAGTC 3 cut(s) 172, 190, 393
PpsI GAGTC 3 cut(s) 172, 190, 393
Psp6I CCWGG 1 cut(s) 40
PspGI CCWGG 1 cut(s) 40
PspN4I GGNNCC 3 cut(s) 33, 120, 515
PspPI GGNCC 1 cut(s) 360
PsuI RGATCY 1 cut(s) 118
RsaI GTAC 1 cut(s) 499
RsaNI GTAC 1 cut(s) 498
SapI GCTCTTC 1 cut(s) 260
SaqAI TTAA 2 cut(s) 414, 544
SatI GCNGC 1 cut(s) 251
Sau3AI GATC 3 cut(s) 118, 350, 381
Sau96I GGNCC 1 cut(s) 360
SchI GAGTC 3 cut(s) 173, 190, 393
ScrFI CCNGG 2 cut(s) 42, 116
SduI GDGCHC 3 cut(s) 34, 94, 518
SetI ASST 7 cut(s) 10, 157, 252, 362, 376, 456, 468
SfaNI GCATC 2 cut(s) 46, 223
SinI GGWCC 1 cut(s) 360
Sse9I AATT 5 cut(s) 15, 80, 109, 265, 271
SspMI CTAG 1 cut(s) 443
StyD4I CCNGG 2 cut(s) 40, 114
StyI CCWWGG 1 cut(s) 356
TaqI TCGA 3 cut(s) 187, 299, 402
TasI AATT 5 cut(s) 15, 80, 109, 265, 271
Tru1I TTAA 2 cut(s) 414, 544
Tru9I TTAA 2 cut(s) 414, 544
TscAI CASTG 2 cut(s) 265, 322
TseFI GTSAC 1 cut(s) 466
TseI GCWGC 1 cut(s) 250
Tsp45I GTSAC 1 cut(s) 466
TspRI CASTG 2 cut(s) 265, 322
Van91I CCANNNNNTGG 1 cut(s) 41
VneI GTGCAC 1 cut(s) 90
VpaK11BI GGWCC 1 cut(s) 360
XapI RAATTY 1 cut(s) 80
XspI CTAG 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.