Rroxscaffold_4G00309920

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000390
Physical Location & Seq
Forward (+)
31773229 .. 31774177
949 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00309920.1

Sequence Viewer

Length: 741 bp
ATGGCCTTGCAGCCAGACGTAGCAGCTCCAGGTACTCACATTTTAGCAGCATATGATCATCAACTGGTTGATGGTGGCAGTAGAGCTGAGTATAGTTTGGAAACTGGGACATCAATGGCATGCCCCCATGCTGCAGGCGTAGCTGCATACGTCAAATCATTTCACTCTAAATGGTCACCAGCTGCTATTCAATCGGCTCTCATCACTACAGCTAAACCTATGAGTGCTAAAACTAGCCCGCACGCTGAATTTGCATATGGAGCTGGCCTACTAAACCCTTCTAGGGCTCCATATCCTGGTTTGGTATACGATCTTGATGAACAAGACTACTTATATTTTTTGTGTTCACAAGGATACAGTGGCAAACTATTGGGGATGATAACCAGGGACAAGAGTAGCTGCTCATCAAAATCTAATAATGAAACGGTCAATGACTTGAACTATCCTTCTTTTGCTCTTTCAATCAAGGATCCAGAATTCATTAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCGTCGAATTCCACATACAGAGCTAAAGTGGTGGCTCCATCAGGACTCAAAATCAATGTGAATCCAAGTGTGCTATCATTCACATCTCTCGGGCAGAAGAAATCTTTTGTTGTTACAGTAAAAGGGTCAATTGAGAAATCAAATATAGTGTCTGCATCTTTGGTGTGGGATGATGGTGTTTTCCAAGTCAGGAGCCCGCTTGTTATCCATGTTATAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

246

Amino Acids

26.28

Weight (kDa)

8.34

Isoelectric Point (pI)

39.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 2 - 89 1.4e-15 Subtilase family
fn3_6 PF17766 145 - 242 9.6e-29 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 296
AccI GTMKAC 1 cut(s) 306
AciI CCGC 2 cut(s) 239, 716
AclWI GGATC 3 cut(s) 464, 477, 526
AcsI RAATTY 3 cut(s) 248, 476, 526
AfaI GTAC 1 cut(s) 34
AfiI CCNNNNNNNGG 2 cut(s) 283, 296
AgsI TTSAA 3 cut(s) 191, 439, 462
AjnI CCWGG 3 cut(s) 28, 295, 383
AluBI AGCT 8 cut(s) 26, 86, 143, 182, 212, 263, 399, 542
AluI AGCT 8 cut(s) 26, 86, 143, 182, 212, 263, 399, 542
AlwI GGATC 3 cut(s) 464, 477, 526
Ama87I CYCGRG 1 cut(s) 608
AoxI GGCC 2 cut(s) 3, 265
ApeKI GCWGC 7 cut(s) 10, 23, 47, 131, 143, 182, 399
ApoI RAATTY 3 cut(s) 248, 476, 526
ArsI GACNNNNNNTTYG 2 cut(s) 653, 685
AseI ATTAAT 1 cut(s) 483
AsuHPI GGTGA 1 cut(s) 168
AvaI CYCGRG 1 cut(s) 608
BamHI GGATCC 1 cut(s) 469
BanII GRGCYC 2 cut(s) 289, 716
BbsI GAAGAC 1 cut(s) 484
BbvI GCAGC 7 cut(s) 22, 35, 59, 118, 130, 169, 386
BccI CCATC 3 cut(s) 65, 565, 686
BciT130I CCWGG 3 cut(s) 30, 297, 385
BciVI GTATCC 1 cut(s) 347
BclI TGATCA 1 cut(s) 55
BfaI CTAG 2 cut(s) 234, 282
BfmI CTRYAG 2 cut(s) 132, 207
BfuI GTATCC 1 cut(s) 347
BisI GCNGC 7 cut(s) 11, 24, 48, 132, 144, 183, 400
BlsI GCNGC 7 cut(s) 12, 25, 49, 133, 145, 184, 401
Bme1390I CCNGG 3 cut(s) 30, 297, 385
BmeT110I CYCGRG 1 cut(s) 608
BmiI GGNNCC 4 cut(s) 288, 471, 555, 713
BmrFI CCNGG 3 cut(s) 30, 297, 385
BmrI ACTGGG 1 cut(s) 114
BmsI GCATC 1 cut(s) 683
BmuI ACTGGG 1 cut(s) 114
BpiI GAAGAC 1 cut(s) 484
BpmI CTGGAG 1 cut(s) 12
BsaJI CCNNGG 1 cut(s) 384
Bsc4I CCNNNNNNNGG 2 cut(s) 283, 296
Bse1I ACTGG 2 cut(s) 69, 109
BseBI CCWGG 3 cut(s) 30, 297, 385
BseDI CCNNGG 1 cut(s) 384
BseGI GGATG 2 cut(s) 381, 694
BseLI CCNNNNNNNGG 2 cut(s) 283, 296
BseMII CTCAG 1 cut(s) 78
BseNI ACTGG 2 cut(s) 69, 109
BseXI GCAGC 7 cut(s) 22, 35, 59, 118, 130, 169, 386
BshFI GGCC 2 cut(s) 5, 267
BsiHKCI CYCGRG 1 cut(s) 608
BslFI GGGAC 2 cut(s) 121, 401
BslI CCNNNNNNNGG 2 cut(s) 283, 296
BsmFI GGGAC 2 cut(s) 121, 401
BsnI GGCC 2 cut(s) 5, 267
BsoBI CYCGRG 1 cut(s) 608
Bsp1286I GDGCHC 2 cut(s) 289, 716
Bsp143I GATC 4 cut(s) 55, 310, 469, 518
BspACI CCGC 2 cut(s) 239, 716
BspANI GGCC 2 cut(s) 5, 267
BspCNI CTCAG 1 cut(s) 79
BspLI GGNNCC 4 cut(s) 288, 471, 555, 713
BspMAI CTGCAG 1 cut(s) 136
BspPI GGATC 3 cut(s) 464, 477, 526
BsrI ACTGG 2 cut(s) 69, 109
BssECI CCNNGG 1 cut(s) 384
BssMI GATC 4 cut(s) 55, 310, 469, 518
BssNAI GTATAC 1 cut(s) 307
Bst1107I GTATAC 1 cut(s) 307
Bst2UI CCWGG 3 cut(s) 30, 297, 385
Bst4CI ACNGT 4 cut(s) 359, 427, 505, 637
BstC8I GCNNGC 6 cut(s) 121, 136, 239, 243, 265, 716
BstDEI CTNAG 1 cut(s) 87
BstEII GGTNACC 1 cut(s) 174
BstF5I GGATG 2 cut(s) 381, 694
BstKTI GATC 4 cut(s) 58, 313, 472, 521
BstMBI GATC 4 cut(s) 55, 310, 469, 518
BstMWI GCNNNNNNNGC 3 cut(s) 140, 251, 260
BstNI CCWGG 3 cut(s) 30, 297, 385
BstNSI RCATGY 1 cut(s) 123
BstPI GGTNACC 1 cut(s) 174
BstSCI CCNGG 3 cut(s) 28, 295, 383
BstSFI CTRYAG 2 cut(s) 132, 207
BstV1I GCAGC 7 cut(s) 22, 35, 59, 118, 130, 169, 386
BstV2I GAAGAC 1 cut(s) 484
BstX2I RGATCY 1 cut(s) 469
BstYI RGATCY 1 cut(s) 469
BstZ17I GTATAC 1 cut(s) 307
BsuI GTATCC 1 cut(s) 347
BsuRI GGCC 2 cut(s) 5, 267
BtsCI GGATG 2 cut(s) 381, 694
BtsIMutI CAGTG 1 cut(s) 364
Cac8I GCNNGC 6 cut(s) 121, 136, 239, 243, 265, 716
Csp6I GTAC 1 cut(s) 33
CviAII CATG 3 cut(s) 120, 128, 728
CviQI GTAC 1 cut(s) 33
DdeI CTNAG 1 cut(s) 87
DpnI GATC 4 cut(s) 57, 312, 471, 520
DpnII GATC 4 cut(s) 55, 310, 469, 518
Eco24I GRGCYC 2 cut(s) 289, 716
Eco88I CYCGRG 1 cut(s) 608
Eco91I GGTNACC 1 cut(s) 174
EcoO65I GGTNACC 1 cut(s) 174
EcoRI GAATTC 2 cut(s) 476, 526
EcoRII CCWGG 3 cut(s) 28, 295, 383
EcoT38I GRGCYC 2 cut(s) 289, 716
FaeI CATG 3 cut(s) 123, 131, 731
FaqI GGGAC 2 cut(s) 121, 401
FatI CATG 3 cut(s) 119, 127, 727
FauI CCCGC 2 cut(s) 246, 723
FauNDI CATATG 2 cut(s) 52, 256
FbaI TGATCA 1 cut(s) 55
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 7 cut(s) 11, 24, 48, 132, 144, 183, 400
FokI GGATG 2 cut(s) 388, 701
FriOI GRGCYC 2 cut(s) 289, 716
Fsp4HI GCNGC 7 cut(s) 11, 24, 48, 132, 144, 183, 400
FspBI CTAG 2 cut(s) 234, 282
GluI GCNGC 7 cut(s) 11, 24, 48, 132, 144, 183, 400
GsuI CTGGAG 1 cut(s) 12
HaeIII GGCC 2 cut(s) 5, 267
Hin1II CATG 3 cut(s) 123, 131, 731
HinfI GANTC 2 cut(s) 564, 580
HphI GGTGA 1 cut(s) 168
Hpy166II GTNNAC 2 cut(s) 307, 347
Hpy188III TCNNGA 4 cut(s) 314, 473, 561, 709
Hpy8I GTNNAC 2 cut(s) 307, 347
Hpy99I CGWCG 1 cut(s) 526
HpyAV CCTTC 2 cut(s) 288, 456
HpyCH4III ACNGT 4 cut(s) 359, 427, 505, 637
HpyCH4IV ACGT 2 cut(s) 18, 150
HpyCH4V TGCA 5 cut(s) 10, 134, 146, 254, 674
HpyF10VI GCNNNNNNNGC 3 cut(s) 140, 251, 260
HpyF3I CTNAG 1 cut(s) 87
HpySE526I ACGT 2 cut(s) 18, 150
Hsp92II CATG 3 cut(s) 123, 131, 731
Ksp22I TGATCA 1 cut(s) 55
Kzo9I GATC 4 cut(s) 55, 310, 469, 518
LmnI GCTCC 5 cut(s) 31, 260, 292, 559, 711
Lsp1109I GCAGC 7 cut(s) 22, 35, 59, 118, 130, 169, 386
LweI GCATC 1 cut(s) 683
MaeI CTAG 2 cut(s) 234, 282
MaeII ACGT 2 cut(s) 18, 150
MaeIII GTNAC 3 cut(s) 174, 505, 631
MalI GATC 4 cut(s) 57, 312, 471, 520
MboI GATC 4 cut(s) 55, 310, 469, 518
MboII GAAGA 2 cut(s) 484, 628
MfeI CAATTG 1 cut(s) 648
MflI RGATCY 1 cut(s) 469
MhlI GDGCHC 2 cut(s) 289, 716
MluCI AATT 4 cut(s) 248, 476, 526, 648
MlyI GAGTC 1 cut(s) 558
MmeI TCCRAC 1 cut(s) 496
MseI TTAA 2 cut(s) 483, 739
MspA1I CMGCKG 1 cut(s) 182
MspR9I CCNGG 3 cut(s) 30, 297, 385
MunI CAATTG 1 cut(s) 648
MvaI CCWGG 3 cut(s) 30, 297, 385
MwoI GCNNNNNNNGC 3 cut(s) 140, 251, 260
NdeI CATATG 2 cut(s) 52, 256
NdeII GATC 4 cut(s) 55, 310, 469, 518
NlaIII CATG 3 cut(s) 123, 131, 731
NlaIV GGNNCC 4 cut(s) 288, 471, 555, 713
NmuCI GTSAC 2 cut(s) 174, 505
NspI RCATGY 1 cut(s) 123
PaeI GCATGC 1 cut(s) 123
PfeI GAWTC 1 cut(s) 580
PflMI CCANNNNNTGG 1 cut(s) 296
PkrI GCNGC 7 cut(s) 12, 25, 49, 133, 145, 184, 401
PleI GAGTC 1 cut(s) 558
PpsI GAGTC 1 cut(s) 558
PshBI ATTAAT 1 cut(s) 483
Psp6I CCWGG 3 cut(s) 28, 295, 383
PspEI GGTNACC 1 cut(s) 174
PspGI CCWGG 3 cut(s) 28, 295, 383
PspN4I GGNNCC 4 cut(s) 288, 471, 555, 713
PstI CTGCAG 1 cut(s) 136
PsuI RGATCY 1 cut(s) 469
PvuII CAGCTG 1 cut(s) 182
RsaI GTAC 1 cut(s) 34
RsaNI GTAC 1 cut(s) 33
SaqAI TTAA 2 cut(s) 483, 739
SatI GCNGC 7 cut(s) 11, 24, 48, 132, 144, 183, 400
Sau3AI GATC 4 cut(s) 55, 310, 469, 518
SchI GAGTC 1 cut(s) 558
ScrFI CCNGG 3 cut(s) 30, 297, 385
SduI GDGCHC 2 cut(s) 289, 716
SfaNI GCATC 1 cut(s) 683
SfcI CTRYAG 2 cut(s) 132, 207
SphI GCATGC 1 cut(s) 123
Sse9I AATT 4 cut(s) 248, 476, 526, 648
SsiI CCGC 2 cut(s) 239, 716
SspMI CTAG 2 cut(s) 234, 282
StyD4I CCNGG 3 cut(s) 28, 295, 383
TaaI ACNGT 4 cut(s) 359, 427, 505, 637
TaiI ACGT 2 cut(s) 21, 153
TaqI TCGA 1 cut(s) 524
TasI AATT 4 cut(s) 248, 476, 526, 648
TfiI GAWTC 1 cut(s) 580
Tru1I TTAA 2 cut(s) 483, 739
Tru9I TTAA 2 cut(s) 483, 739
TscAI CASTG 1 cut(s) 364
TseFI GTSAC 2 cut(s) 174, 505
TseI GCWGC 7 cut(s) 10, 23, 47, 131, 143, 182, 399
Tsp45I GTSAC 2 cut(s) 174, 505
TspDTI ATGAA 3 cut(s) 333, 435, 469
TspRI CASTG 1 cut(s) 364
Van91I CCANNNNNTGG 1 cut(s) 296
VspI ATTAAT 1 cut(s) 483
XapI RAATTY 3 cut(s) 248, 476, 526
XceI RCATGY 1 cut(s) 123
XmiI GTMKAC 1 cut(s) 306
XspI CTAG 2 cut(s) 234, 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.