RchiOBHm_Chr1g0370161

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000390
Physical Location & Seq
Reverse (-)
59703470 .. 59707138
3669 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59434

Sequence Viewer

Length: 2256 bp
ATGCCTCTAGCTAGTAGCACCATACAGAGTAATAGTATGGCTCTTCGATGGTTTCTCCTTCTCAACCTCATTTGCAGTATTCTACTGCTTGTCGATGTTACTCATTCAGCTGCTCAGGATGCCCGAAAGGCTTATGTTGTGTATATGGGCGGCAAGCCAAAGAATGGGGTACCCATATTACCTGATCTTCATGTGAATATGCTACAAGACGTAGTCGACAGTAGCAACATTGATATTGCACATGAAGATCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATGATTGGAATGGATGGTGTAGTGTCTGTCTTCCCAAGCAAACAGAGGAGCGTCAAAACAACAAAGTCATGGAACTTTGTTGGGTTTCCAGAAACAGTTAAGAGAGGCGCCCTTGAAAGGGATATCATTGTTGGTGTGATAGACAGTGGAATTTGGCCAGAGTCGGACAGCTTTAGTGATGTTGGTTTTGGTCCCCCACCCAAGAAATGGAAAGGCAAATGTCAAGGCAATGGAAATCTTACTTGTAACAATAAAATCATTGGAGCACAATATTATCATGCTTCTCAATCATTCAAAGAAGATGTTGAGTCTCCAAGAGACTCAAACGGTCATGGAACTCACACCGCATCAACAGCAGCTGGGAACACAGTGATCAAGGTGAGCTTTAATGGTTTAGGGTCGGGAACAGCAAGAGGAGGGGTGCCATCAGCGCGTATTGCTGTGTACAAAGCATGTTGGGGCAGCAGTGGCAGTTGTGACGATGCTGATGTTCTAGCCGCATTCGATGATGCCATTGCTGACGGTGTAGACATTCTCTCTGTTTCCCTCGGGGGCACTATTCCATTTGATTATTTGAAGGATTCATTTGCAATTGGGTCATTTCACGCTACCAGAAATGGGATATTAGTTTCTATGGCAGCTGGTAACGAAGGTCCGGGCGCAAAAACTGTGACAAACTTTGCACCATGGCAACTTTCTGTGGCTGCTACCACCATAAACCGCCAATTCATCACCAAGGTTCAATTGGGTAACGGTAAAATCTATGAGGGACTTTTACCAAACATATATGGCCTCCGGGGTAAATTCTATCCTTTAATTTATGGTGGAGACGCGCCCAATACAAAAGCAGGTGTTGACGGGAGTATCTCTAGGTTTTGTGGAAGAGATGACGTAGACGAAGATTTGATCAAAGGTAAAATTGTGTTGTGTGATGGCCCTATGGATGAGGCTGGGGTTGGGGCGATATTGGGTGGCGCAGCCGGAATTGTTCTGACAGGAAAAAAAGTCAACGATGAACTGCTTGGCCCTTATCCGGTGCCTGCAACTTTCGTCGGGTTGGAAGAAAACAGCGACATTTACAAATACATAAATTCAACAAGGAATGCAACTGCAACTATATGGAAAAGTGAAGAGATTAGTGATGTATTGGCTCCATACGTGCCATCCTACTCATCGAGGGGCCCAAATCCGATCAATCCCAACATTCTCAAGCCAGATTTAGCGGCTCCAGGAACTTACATTCTGGCAGCATATCCCCCAAGTGGTGGTGGTAGTAGAGCTAGATACTATATAGAAACTGGGACATCAATGGCATGCCCCCATGCTACCGCCGTAGCTGCATACGTCAAATCATTTCACCCTAAATGGTCACCAGCTGCTATCCAATCGGCTCTCATCACTACTGCTAAACCTATGAGTGCCAAAACTAGCCCTGACGCTGAATTTGCATACGGAGCTGGCCTAATAAACCCTTCTAGGGCGCCATATCCTGGTTTGGTGTATGATCTTGATGGACAAGACTACATAGATTTTTTGTGTTCACAAGGATACAATGGTAAACTATTGCAAGCCATAACCATGGACAAAACTAGTTGCTCATCAAAATCTATTAATAGAACAGCTAATGACATGAACTATCCTTCTTTTGCACTTTCCATCAAGGATTCGAAATTTGTCAATGGGGTCTTCCATAGAACTGTCACTAATGTTGGATCTTCAAAATCCACATACAGAGCTAAAACGGTGGCTCCATTGGGACTCAAAATCAATGTGAATCCAAGTGTGCTATCGTTCACATATCTCGGGCAAAAGAAATCTTTTGTTGTCACTATAAAAGGGCCGATTAAGAAATCAAATCTAATCTCTGCGTCTTTGGTGTGGGATGATGGTGCTTTCCAAGTCAGGAGCCCCATTGTTGTCTATGTTGCTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

751

Amino Acids

80.06

Weight (kDa)

8.53

Isoelectric Point (pI)

34.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 45 - 124 2.1e-12 Peptidase inhibitor I9
Peptidase_S8 PF00082 147 - 594 3.2e-43 Subtilase family
fn3_6 PF17766 650 - 747 1.3e-26 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1160
Acc36I ACCTGC 1 cut(s) 1160
Acc65I GGTACC 1 cut(s) 169
AccB1I GGYRCC 5 cut(s) 169, 428, 742, 1357, 1801
AccB7I CCANNNNNTGG 4 cut(s) 164, 528, 1586, 1811
AccI GTMKAC 3 cut(s) 216, 849, 1215
AccII CGCG 2 cut(s) 754, 1154
AciI CCGC 6 cut(s) 150, 666, 819, 1042, 1544, 1650
AclWI GGATC 1 cut(s) 2041
AcoI YGGCCR 1 cut(s) 476
AcsI RAATTY 5 cut(s) 471, 1124, 1411, 1763, 1991
AcyI GRCGYC 2 cut(s) 429, 1802
AfaI GTAC 2 cut(s) 171, 767
AgsI TTSAA 7 cut(s) 280, 437, 616, 898, 1064, 1416, 2040
AhlI ACTAGT 1 cut(s) 1910
AjnI CCWGG 2 cut(s) 1549, 1810
Alw21I GWGCWC 1 cut(s) 589
Alw26I GTCTC 3 cut(s) 633, 636, 1143
AlwI GGATC 1 cut(s) 2041
AlwNI CAGNNNCTG 1 cut(s) 680
Ama87I CYCGRG 2 cut(s) 869, 2123
AoxI GGCC 7 cut(s) 476, 1111, 1255, 1345, 1501, 1780, 2159
ApaI GGGCCC 1 cut(s) 1505
ApoI RAATTY 5 cut(s) 471, 1124, 1411, 1763, 1991
ArsI GACNNNNNNTTYG 2 cut(s) 1748, 1780
AseI ATTAAT 1 cut(s) 1932
Asp718I GGTACC 1 cut(s) 169
AspLEI GCGC 6 cut(s) 431, 754, 983, 1156, 1298, 1804
AspS9I GGNCC 7 cut(s) 512, 974, 1256, 1346, 1501, 1502, 2159
AsuC2I CCSGG 2 cut(s) 978, 1118
AsuHPI GGTGA 4 cut(s) 712, 1045, 1670, 1683
AsuII TTCGAA 1 cut(s) 1988
AvaI CYCGRG 2 cut(s) 869, 2123
AvaII GGWCC 2 cut(s) 512, 974
BaeGI GKGCMC 2 cut(s) 878, 1505
BaeI ACNNNNGTAYC 4 cut(s) 1168, 1201, 1861, 1894
BalI TGGCCA 1 cut(s) 478
BanI GGYRCC 5 cut(s) 169, 428, 742, 1357, 1801
BanII GRGCYC 2 cut(s) 1505, 2231
BbsI GAAGAC 2 cut(s) 343, 1999
Bbv12I GWGCWC 1 cut(s) 589
BccI CCATC 8 cut(s) 42, 329, 754, 1247, 1492, 1826, 1985, 2201
BceAI ACGGC 1 cut(s) 1637
BciT130I CCWGG 2 cut(s) 1551, 1812
BciVI GTATCC 1 cut(s) 1862
BclI TGATCA 2 cut(s) 693, 1227
BcnI CCSGG 2 cut(s) 978, 1118
BcoDI GTCTC 3 cut(s) 633, 636, 1143
BcuI ACTAGT 1 cut(s) 1910
BfaI CTAG 8 cut(s) 8, 12, 815, 1191, 1602, 1749, 1797, 1911
BfoI RGCGCY 2 cut(s) 432, 1805
BfuAI ACCTGC 1 cut(s) 1160
BfuI GTATCC 1 cut(s) 1862
BglI GCCNNNNNGGC 1 cut(s) 128
BglII AGATCT 1 cut(s) 247
Bme1390I CCNGG 4 cut(s) 978, 1118, 1551, 1812
Bme18I GGWCC 2 cut(s) 512, 974
BmeT110I CYCGRG 2 cut(s) 869, 2123
BmgT120I GGNCC 7 cut(s) 512, 974, 1256, 1346, 1501, 1502, 2159
BmrFI CCNGG 4 cut(s) 978, 1118, 1551, 1812
BmrI ACTGGG 1 cut(s) 1629
BmsI GCATC 4 cut(s) 109, 677, 793, 820
BmuI ACTGGG 1 cut(s) 1629
BpiI GAAGAC 2 cut(s) 343, 1999
BpmI CTGGAG 1 cut(s) 1533
Bpu10I CCTNAGC 1 cut(s) 114
Bpu14I TTCGAA 1 cut(s) 1988
BpuEI CTTGAG 1 cut(s) 1514
BpuMI CCSGG 2 cut(s) 978, 1118
BsaAI YACGTR 1 cut(s) 1480
BsaHI GRCGYC 2 cut(s) 429, 1802
BsaJI CCNNGG 5 cut(s) 868, 1007, 1056, 1117, 1899
BsaWI WCCGGW 1 cut(s) 1354
BsaXI ACNNNNNCTCC 2 cut(s) 2053, 2083
Bse1I ACTGG 1 cut(s) 1624
Bse3DI GCAATG 3 cut(s) 300, 556, 834
BseBI CCWGG 2 cut(s) 1551, 1812
BseDI CCNNGG 5 cut(s) 868, 1007, 1056, 1117, 1899
BseGI GGATG 5 cut(s) 124, 340, 1270, 1484, 2209
BseMI GCAATG 3 cut(s) 300, 556, 834
BseMII CTCAG 1 cut(s) 128
BseNI ACTGG 1 cut(s) 1624
BseRI GAGGAG 2 cut(s) 382, 750
BseSI GKGCMC 2 cut(s) 878, 1505
BseYI CCCAGC 2 cut(s) 680, 1271
Bsh1236I CGCG 2 cut(s) 754, 1154
BshFI GGCC 7 cut(s) 478, 1113, 1257, 1347, 1503, 1782, 2161
BshNI GGYRCC 5 cut(s) 169, 428, 742, 1357, 1801
BsiHKAI GWGCWC 1 cut(s) 589
BsiHKCI CYCGRG 2 cut(s) 869, 2123
BsiSI CCGG 4 cut(s) 977, 1117, 1302, 1355
BslFI GGGAC 4 cut(s) 498, 1104, 1636, 2091
BsmAI GTCTC 3 cut(s) 633, 636, 1143
BsmBI CGTCTC 1 cut(s) 1143
BsmFI GGGAC 4 cut(s) 498, 1104, 1636, 2091
BsmI GAATGC 2 cut(s) 821, 1429
BsnI GGCC 7 cut(s) 478, 1113, 1257, 1347, 1503, 1782, 2161
BsoBI CYCGRG 2 cut(s) 869, 2123
Bsp119I TTCGAA 1 cut(s) 1988
Bsp120I GGGCCC 1 cut(s) 1501
Bsp1286I GDGCHC 4 cut(s) 589, 878, 1505, 2231
Bsp1407I TGTACA 1 cut(s) 765
Bsp143I GATC 7 cut(s) 184, 247, 693, 1227, 1512, 1825, 2033
Bsp19I CCATGG 2 cut(s) 1007, 1899
BspACI CCGC 6 cut(s) 150, 666, 819, 1042, 1544, 1650
BspANI GGCC 7 cut(s) 478, 1113, 1257, 1347, 1503, 1782, 2161
BspCNI CTCAG 1 cut(s) 127
BspFNI CGCG 2 cut(s) 754, 1154
BspMI ACCTGC 1 cut(s) 1160
BspPI GGATC 1 cut(s) 2041
BspQI GCTCTTC 1 cut(s) 48
BspT104I TTCGAA 1 cut(s) 1988
BspT107I GGYRCC 5 cut(s) 169, 428, 742, 1357, 1801
BsrDI GCAATG 3 cut(s) 300, 556, 834
BsrGI TGTACA 1 cut(s) 765
BsrI ACTGG 1 cut(s) 1624
BssECI CCNNGG 5 cut(s) 868, 1007, 1056, 1117, 1899
BssMI GATC 7 cut(s) 184, 247, 693, 1227, 1512, 1825, 2033
BssNI GRCGYC 2 cut(s) 429, 1802
BssT1I CCWWGG 3 cut(s) 1007, 1056, 1899
Bst2UI CCWGG 2 cut(s) 1551, 1812
Bst6I CTCTTC 3 cut(s) 48, 1198, 1446
BstACI GRCGYC 2 cut(s) 429, 1802
BstAUI TGTACA 1 cut(s) 765
BstBAI YACGTR 1 cut(s) 1480
BstBI TTCGAA 1 cut(s) 1988
BstC8I GCNNGC 5 cut(s) 155, 1362, 1636, 1780, 1890
BstDEI CTNAG 1 cut(s) 114
BstDSI CCRYGG 2 cut(s) 1007, 1899
BstEII GGTNACC 1 cut(s) 1689
BstF5I GGATG 5 cut(s) 124, 340, 1270, 1484, 2209
BstFNI CGCG 2 cut(s) 754, 1154
BstH2I RGCGCY 2 cut(s) 432, 1805
BstHHI GCGC 6 cut(s) 431, 754, 983, 1156, 1298, 1804
BstKTI GATC 7 cut(s) 187, 250, 696, 1230, 1515, 1828, 2036
BstMAI GTCTC 3 cut(s) 633, 636, 1143
BstMBI GATC 7 cut(s) 184, 247, 693, 1227, 1512, 1825, 2033
BstNI CCWGG 2 cut(s) 1551, 1812
BstNSI RCATGY 2 cut(s) 777, 1638
BstPI GGTNACC 1 cut(s) 1689
BstSCI CCNGG 4 cut(s) 976, 1116, 1549, 1810
BstSLI GKGCMC 2 cut(s) 878, 1505
BstUI CGCG 2 cut(s) 754, 1154
BstV2I GAAGAC 2 cut(s) 343, 1999
BstX2I RGATCY 2 cut(s) 247, 2033
BstXI CCANNNNNNTGG 1 cut(s) 1900
BstYI RGATCY 2 cut(s) 247, 2033
BsuI GTATCC 1 cut(s) 1862
BsuRI GGCC 7 cut(s) 478, 1113, 1257, 1347, 1503, 1782, 2161
BtgI CCRYGG 2 cut(s) 1007, 1899
BtsCI GGATG 5 cut(s) 124, 340, 1270, 1484, 2209
BtsI GCAGTG 1 cut(s) 793
BtsIMutI CAGTG 3 cut(s) 472, 696, 793
BveI ACCTGC 1 cut(s) 1160
Cac8I GCNNGC 5 cut(s) 155, 1362, 1636, 1780, 1890
CaiI CAGNNNCTG 1 cut(s) 680
CfoI GCGC 6 cut(s) 431, 754, 983, 1156, 1298, 1804
Cfr13I GGNCC 7 cut(s) 512, 974, 1256, 1346, 1501, 1502, 2159
CseI GACGC 4 cut(s) 361, 1160, 1766, 2178
Csp6I GTAC 2 cut(s) 170, 766
CviQI GTAC 2 cut(s) 170, 766
DdeI CTNAG 1 cut(s) 114
DinI GGCGCC 2 cut(s) 430, 1803
DpnI GATC 7 cut(s) 186, 249, 695, 1229, 1514, 1827, 2035
DpnII GATC 7 cut(s) 184, 247, 693, 1227, 1512, 1825, 2033
EaeI YGGCCR 1 cut(s) 476
Eam1104I CTCTTC 3 cut(s) 48, 1198, 1446
EarI CTCTTC 3 cut(s) 48, 1198, 1446
Eco130I CCWWGG 3 cut(s) 1007, 1056, 1899
Eco24I GRGCYC 2 cut(s) 1505, 2231
Eco32I GATATC 1 cut(s) 445
Eco47I GGWCC 2 cut(s) 512, 974
Eco88I CYCGRG 2 cut(s) 869, 2123
Eco91I GGTNACC 1 cut(s) 1689
EcoO109I RGGNCCY 1 cut(s) 1501
EcoO65I GGTNACC 1 cut(s) 1689
EcoRII CCWGG 2 cut(s) 1549, 1810
EcoRV GATATC 1 cut(s) 445
EcoT14I CCWWGG 3 cut(s) 1007, 1056, 1899
EcoT38I GRGCYC 2 cut(s) 1505, 2231
EgeI GGCGCC 2 cut(s) 430, 1803
EheI GGCGCC 2 cut(s) 430, 1803
ErhI CCWWGG 3 cut(s) 1007, 1056, 1899
Esp3I CGTCTC 1 cut(s) 1143
FalI AAGNNNNNCTT 4 cut(s) 237, 269, 689, 721
FaqI GGGAC 4 cut(s) 498, 1104, 1636, 2091
FbaI TGATCA 2 cut(s) 693, 1227
FblI GTMKAC 3 cut(s) 216, 849, 1215
FokI GGATG 5 cut(s) 131, 347, 1277, 1471, 2216
FriOI GRGCYC 2 cut(s) 1505, 2231
FspBI CTAG 8 cut(s) 8, 12, 815, 1191, 1602, 1749, 1797, 1911
GlaI GCGC 6 cut(s) 430, 753, 982, 1155, 1297, 1803
GsaI CCCAGC 2 cut(s) 684, 1275
GsuI CTGGAG 1 cut(s) 1533
HaeII RGCGCY 2 cut(s) 432, 1805
HaeIII GGCC 7 cut(s) 478, 1113, 1257, 1347, 1503, 1782, 2161
HapII CCGG 4 cut(s) 977, 1117, 1302, 1355
HgaI GACGC 4 cut(s) 361, 1160, 1766, 2178
HhaI GCGC 6 cut(s) 431, 754, 983, 1156, 1298, 1804
Hin1I GRCGYC 2 cut(s) 429, 1802
Hin6I GCGC 6 cut(s) 429, 752, 981, 1154, 1296, 1802
HinP1I GCGC 6 cut(s) 429, 752, 981, 1154, 1296, 1802
HincII GTYRAC 3 cut(s) 217, 1177, 1330
HindII GTYRAC 3 cut(s) 217, 1177, 1330
HinfI GANTC 7 cut(s) 482, 629, 641, 902, 1985, 2079, 2095
HpaII CCGG 4 cut(s) 977, 1117, 1302, 1355
HphI GGTGA 4 cut(s) 712, 1045, 1670, 1683
Hpy166II GTNNAC 9 cut(s) 217, 766, 850, 1177, 1216, 1330, 1862, 1880, 2115
Hpy188I TCNGA 3 cut(s) 487, 1314, 1512
Hpy188III TCNNGA 5 cut(s) 116, 410, 723, 1829, 2224
Hpy8I GTNNAC 9 cut(s) 217, 766, 850, 1177, 1216, 1330, 1862, 1880, 2115
Hpy99I CGWCG 1 cut(s) 1376
HpyAV CCTTC 5 cut(s) 68, 892, 965, 1803, 1971
HpyCH4IV ACGT 4 cut(s) 210, 1212, 1479, 1665
HpyF3I CTNAG 1 cut(s) 114
HpySE526I ACGT 4 cut(s) 210, 1212, 1479, 1665
Hsp92I GRCGYC 2 cut(s) 429, 1802
HspAI GCGC 6 cut(s) 429, 752, 981, 1154, 1296, 1802
KasI GGCGCC 2 cut(s) 428, 1801
KpnI GGTACC 1 cut(s) 173
Ksp22I TGATCA 2 cut(s) 693, 1227
Kzo9I GATC 7 cut(s) 184, 247, 693, 1227, 1512, 1825, 2033
LguI GCTCTTC 1 cut(s) 48
LmnI GCTCC 7 cut(s) 369, 584, 1477, 1552, 1775, 2074, 2226
LweI GCATC 4 cut(s) 109, 677, 793, 820
MaeI CTAG 8 cut(s) 8, 12, 815, 1191, 1602, 1749, 1797, 1911
MaeII ACGT 4 cut(s) 210, 1212, 1479, 1665
MaeIII GTNAC 9 cut(s) 97, 566, 797, 965, 991, 1070, 1689, 2020, 2146
MalI GATC 7 cut(s) 186, 249, 695, 1229, 1514, 1827, 2035
MboI GATC 7 cut(s) 184, 247, 693, 1227, 1512, 1825, 2033
MfeI CAATTG 2 cut(s) 912, 1064
MflI RGATCY 2 cut(s) 247, 2033
MhlI GDGCHC 4 cut(s) 589, 878, 1505, 2231
MlsI TGGCCA 1 cut(s) 478
MluNI TGGCCA 1 cut(s) 478
Mly113I GGCGCC 2 cut(s) 429, 1802
MlyI GAGTC 4 cut(s) 491, 635, 638, 2073
MmeI TCCRAC 3 cut(s) 465, 1359, 2011
Mox20I TGGCCA 1 cut(s) 478
MscI TGGCCA 1 cut(s) 478
MseI TTAA 6 cut(s) 420, 708, 1136, 1932, 2166, 2254
MslI CAYNNNNRTG 1 cut(s) 1898
Msp20I TGGCCA 1 cut(s) 478
MspA1I CMGCKG 4 cut(s) 110, 680, 962, 1697
MspI CCGG 4 cut(s) 977, 1117, 1302, 1355
MspR9I CCNGG 4 cut(s) 978, 1118, 1551, 1812
MunI CAATTG 2 cut(s) 912, 1064
Mva1269I GAATGC 2 cut(s) 821, 1429
MvaI CCWGG 2 cut(s) 1551, 1812
MvnI CGCG 2 cut(s) 754, 1154
NarI GGCGCC 2 cut(s) 429, 1802
NciI CCSGG 2 cut(s) 978, 1118
NcoI CCATGG 2 cut(s) 1007, 1899
NdeII GATC 7 cut(s) 184, 247, 693, 1227, 1512, 1825, 2033
NmuCI GTSAC 5 cut(s) 797, 991, 1689, 2020, 2146
NspI RCATGY 2 cut(s) 777, 1638
NspV TTCGAA 1 cut(s) 1988
PaeI GCATGC 1 cut(s) 1638
PaqCI CACCTGC 1 cut(s) 1160
PciSI GCTCTTC 1 cut(s) 48
PctI GAATGC 2 cut(s) 821, 1429
PfeI GAWTC 3 cut(s) 902, 1985, 2095
PflFI GACNNNGTC 1 cut(s) 212
PflMI CCANNNNNTGG 4 cut(s) 164, 528, 1586, 1811
PfoI TCCNGGA 1 cut(s) 1549
PleI GAGTC 4 cut(s) 490, 635, 637, 2073
PluTI GGCGCC 2 cut(s) 432, 1805
PpsI GAGTC 4 cut(s) 490, 635, 637, 2073
Ppu21I YACGTR 1 cut(s) 1480
PshBI ATTAAT 1 cut(s) 1932
Psp6I CCWGG 2 cut(s) 1549, 1810
PspEI GGTNACC 1 cut(s) 1689
PspFI CCCAGC 2 cut(s) 680, 1271
PspGI CCWGG 2 cut(s) 1549, 1810
PspOMI GGGCCC 1 cut(s) 1501
PspPI GGNCC 7 cut(s) 512, 974, 1256, 1346, 1501, 1502, 2159
PstNI CAGNNNCTG 1 cut(s) 680
PsuI RGATCY 2 cut(s) 247, 2033
PsyI GACNNNGTC 1 cut(s) 212
PvuII CAGCTG 4 cut(s) 110, 680, 962, 1697
RsaI GTAC 2 cut(s) 171, 767
RsaNI GTAC 2 cut(s) 170, 766
RseI CAYNNNNRTG 1 cut(s) 1898
SalI GTCGAC 1 cut(s) 215
SapI GCTCTTC 1 cut(s) 48
SaqAI TTAA 6 cut(s) 420, 708, 1136, 1932, 2166, 2254
Sau3AI GATC 7 cut(s) 184, 247, 693, 1227, 1512, 1825, 2033
Sau96I GGNCC 7 cut(s) 512, 974, 1256, 1346, 1501, 1502, 2159
SchI GAGTC 4 cut(s) 491, 635, 638, 2073
ScrFI CCNGG 4 cut(s) 978, 1118, 1551, 1812
SduI GDGCHC 4 cut(s) 589, 878, 1505, 2231
SfaNI GCATC 4 cut(s) 109, 677, 793, 820
SfoI GGCGCC 2 cut(s) 430, 1803
SfuI TTCGAA 1 cut(s) 1988
SinI GGWCC 2 cut(s) 512, 974
SmiMI CAYNNNNRTG 1 cut(s) 1898
SmlI CTYRAG 1 cut(s) 1529
SmoI CTYRAG 1 cut(s) 1529
SpeI ACTAGT 1 cut(s) 1910
SphI GCATGC 1 cut(s) 1638
SsiI CCGC 6 cut(s) 150, 666, 819, 1042, 1544, 1650
SspDI GGCGCC 2 cut(s) 428, 1801
SspI AATATT 1 cut(s) 593
SspMI CTAG 8 cut(s) 8, 12, 815, 1191, 1602, 1749, 1797, 1911
StyD4I CCNGG 4 cut(s) 976, 1116, 1549, 1810
StyI CCWWGG 3 cut(s) 1007, 1056, 1899
TaiI ACGT 4 cut(s) 213, 1215, 1482, 1668
TaqI TCGA 6 cut(s) 46, 93, 216, 825, 1496, 1988
TatI WGTACW 1 cut(s) 765
TauI GCSGC 3 cut(s) 153, 821, 1547
TfiI GAWTC 3 cut(s) 902, 1985, 2095
Tru1I TTAA 6 cut(s) 420, 708, 1136, 1932, 2166, 2254
Tru9I TTAA 6 cut(s) 420, 708, 1136, 1932, 2166, 2254
TscAI CASTG 3 cut(s) 472, 696, 793
TseFI GTSAC 5 cut(s) 797, 991, 1689, 2020, 2146
Tsp45I GTSAC 5 cut(s) 797, 991, 1689, 2020, 2146
TspDTI ATGAA 6 cut(s) 179, 258, 894, 1039, 1350, 1967
TspGWI ACGGA 1 cut(s) 1788
TspRI CASTG 3 cut(s) 472, 696, 793
Tth111I GACNNNGTC 1 cut(s) 212
Van91I CCANNNNNTGG 4 cut(s) 164, 528, 1586, 1811
VpaK11BI GGWCC 2 cut(s) 512, 974
VspI ATTAAT 1 cut(s) 1932
XapI RAATTY 5 cut(s) 471, 1124, 1411, 1763, 1991
XceI RCATGY 2 cut(s) 777, 1638
XcmI CCANNNNNNNNNTGG 2 cut(s) 525, 1063
XmiI GTMKAC 3 cut(s) 216, 849, 1215
XspI CTAG 8 cut(s) 8, 12, 815, 1191, 1602, 1749, 1797, 1911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.