RLG00000030097

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000390
Physical Location & Seq
Forward (+)
55123524 .. 55128183
4660 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030097

Sequence Viewer

Length: 2211 bp
ATGGCTCTTCGATGGTTTCTCCTTCTCAGCCTCATTTGCGGTGTTCTACTGCTTGTCGATGTTACTCACTTAGCTGCTCAGGATACCCGAAAGGCTTATGTTGTGTATATGGGCGACAAGCCAAAGAACAGGGTACCCATATTACGTGATCTTCATGTGAATATGCTACAGGACGTAGTTGACAGTAGCAACAATGTTATTGCACATGAAGCTCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGTTCACAGAGCAGGAAGCACAAAAAATAACTGGAATGGATGGTGTAGTGTCTGTCTTCCCAAGCAAACAAAGGATCGTCAAAACAACAAAGTCATGGAACTTCCTTGGGTTTCCAGAAACAGTTAAGAGAAGCACTCTTGAAAGGGATATCATAGTCGGTGTGATGGACACTGGAATTTGGCCAGAGTCAGACAGCTTTAATGATGCTGGTTTTGGTCCTCCACCTAAGAAATGGAAAGGCAAATGTCAAGGCAATGGAAATCTTACTTGTAACAATAAAATCATCGGAGCACAGTATTATCGTGCTTCTCAATCCTTCGAAGAAGATATTGAGTCTCCAAGAGACTCAAGTGGTCATGGAACTCATACCGCATCAACGGCAGCTGGGAACACAGTGAGCAAGGCAAGCCTAAACGGTTTAGCTTTGGGAACAGCAAGAGGAGGGGTGCCATCAGCACGTATTGCCGTGTACAAAGTATGCTGGAGCAACACGCCTGCTTGTGCAGATGCTGACATTTTAGCGGCATTCGATGATGCCATTGCTGACGGTGTCGACATTCTCTCTATTTCCATCGGGGGTAGAATTCCAGTTGATTATTTGACTAATTCATTTGCAATTGGGTCATTTCATGCTACCAGAAATGGGATATTAGTTTCCATGTCCGCTGGTAATGAGGGTCCGGGAGCAAAAACTGTGACAAACTTTGCACCATGGCAACTTTCTGTAGCTGCTACCACCATAAACCGTCAATTCATCACCAAGGTGCAATTGGGTAACGGTAAAATCTATGAGGGACTTTTACTAAACATATATGACCTCCAGCGTAAATTCTATCCTTTAATTTATGGTGGAGACGCACCCAATAAAAAAGCAGGTTTTAAGGGGACCGTCTCTAGGTTTTGTGGCATATCTACCTTAGAAGAAGATTTGATCAAAGATAAAATTGTGCTTTGTGATGGCCCAGTTGGGTTTGGGGCCATATTGGGTGGTGCAGCAGGAATGATTCTGACAGGACAAAAAGTCGCCAATGAAGTGCCTCCCCTTTTTCCTGTGCCTGCATCACAAGTCGGGTTGGAAGAAAATAGCGACATTTACAAATACATAAACTTAACAAGGAATGCAACTGCAACTATTTGGAAAGCTGAAGAGATTAATGATATATTGGCTCCATATGTGCCCTCCTACTCATCAAGGGGTCCGAATCCGATCAATCCTAACATTCTCAAGCCAGATTTAGCGGCACCAGGAACTTACATTCTGGCAGCATATCCCCCAAGTGGTGGTGGCAGTAGAGCTGGATACTATATAGAGACTGGGACATCAATGGCATGCCCCCATGCTACGGCCGTAGCTGTATACGTCAAATCATTTCATCCTAAATGGTCACCGGCTGCTATCCAATCGGCTCTCATCACTACCGCTAAATCTATGAGTGCCAAAACTAGCCCTGATGCTGAATTTGCATACGGAGCTGGCCTGATAAACCCTTCTAGGGCTCCATATCCTGGTTTGGTGTACGATCTTGGTGAACAAGACTACATAGATTTTTTGTGTTCACAAGGATACAGTGGTAAACTACTGCAAGCCATAACTGGGGATAAGACTAGTTGTTCATCAAAATCTAATAATGGAACAGCCAATGACCTGAACTATCCTTCTTTTGCACTTTCCATTAAGAATCCGAAATTTGTCAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCATCGAATTCCACATACAGAGTTAAAGTGGTGGCTCCATTGGGACTCAAAATCAATGTGAATCCAAGTGTGCTATCATTCACATCTCTCGGGCAAAAGAAATCTTTTGTTGTCACTATAAAAGGGCCGATTGAGAAATCAAATTTAGTCTCTGCATCTTTGGTGTGGGATGATGGTGTTTTCCAAGTCAAGAGCCCCATCGTTGTCTATGTTGCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

737

Amino Acids

78.78

Weight (kDa)

8.85

Isoelectric Point (pI)

35.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 110 2.4e-14 Peptidase inhibitor I9
Peptidase_S8 PF00082 135 - 579 4.5e-43 Subtilase family
fn3_6 PF17766 635 - 732 3.3e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1124
Acc65I GGTACC 1 cut(s) 133
AccB1I GGYRCC 3 cut(s) 133, 706, 1501
AccB7I CCANNNNNTGG 2 cut(s) 1541, 1766
AccI GTMKAC 2 cut(s) 813, 1617
AciI CCGC 6 cut(s) 39, 630, 782, 924, 1499, 1680
AclWI GGATC 2 cut(s) 341, 1996
AcoI YGGCCR 2 cut(s) 440, 1605
AcsI RAATTY 7 cut(s) 435, 843, 1088, 1718, 1946, 1996, 2131
AcuI CTGAAG 1 cut(s) 1425
AfaI GTAC 3 cut(s) 135, 731, 1778
AfiI CCNNNNNNNGG 8 cut(s) 903, 1155, 1538, 1541, 1603, 1753, 1766, 1854
AgsI TTSAA 2 cut(s) 244, 401
AhdI GACNNNNNGTC 1 cut(s) 1280
AhlI ACTAGT 1 cut(s) 1865
AjnI CCWGG 2 cut(s) 1504, 1765
AleI CACNNNNGTG 1 cut(s) 1022
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 6 cut(s) 597, 600, 1107, 1156, 1565, 2143
AlwI GGATC 2 cut(s) 341, 1996
AlwNI CAGNNNCTG 1 cut(s) 770
Ama87I CYCGRG 1 cut(s) 2078
AoxI GGCC 6 cut(s) 440, 1219, 1236, 1605, 1735, 2114
ApeKI GCWGC 7 cut(s) 74, 254, 641, 989, 1253, 1523, 1652
ApoI RAATTY 7 cut(s) 435, 843, 1088, 1718, 1946, 1996, 2131
AseI ATTAAT 1 cut(s) 1413
Asp718I GGTACC 1 cut(s) 133
AspS9I GGNCC 7 cut(s) 476, 938, 1146, 1220, 1236, 1457, 2114
AsuC2I CCSGG 1 cut(s) 942
AsuHPI GGTGA 3 cut(s) 1009, 1638, 1799
AsuII TTCGAA 1 cut(s) 579
AvaI CYCGRG 1 cut(s) 2078
AvaII GGWCC 4 cut(s) 476, 938, 1146, 1457
BaeGI GKGCMC 1 cut(s) 1440
BaeI ACNNNNGTAYC 2 cut(s) 1816, 1849
BalI TGGCCA 1 cut(s) 442
BanI GGYRCC 3 cut(s) 133, 706, 1501
BanII GRGCYC 2 cut(s) 1759, 2186
BbsI GAAGAC 2 cut(s) 307, 1954
Bbv12I GWGCWC 1 cut(s) 553
BbvI GCAGC 7 cut(s) 61, 241, 653, 976, 1265, 1535, 1639
BccI CCATC 8 cut(s) 6, 293, 418, 718, 839, 1211, 2156, 2195
BceAI ACGGC 4 cut(s) 654, 710, 1592, 1620
BciT130I CCWGG 2 cut(s) 1506, 1767
BciVI GTATCC 3 cut(s) 76, 1553, 1817
BclI TGATCA 1 cut(s) 1191
BcnI CCSGG 1 cut(s) 942
BcoDI GTCTC 6 cut(s) 597, 600, 1107, 1156, 1565, 2143
BcuI ACTAGT 1 cut(s) 1865
BfaI CTAG 4 cut(s) 1155, 1704, 1752, 1866
BfmI CTRYAG 2 cut(s) 167, 984
BfuAI ACCTGC 1 cut(s) 1124
BfuI GTATCC 3 cut(s) 76, 1553, 1817
BisI GCNGC 9 cut(s) 75, 255, 642, 783, 990, 1254, 1500, 1524, 1653
BlsI GCNGC 9 cut(s) 76, 256, 643, 784, 991, 1255, 1501, 1525, 1654
Bme1390I CCNGG 3 cut(s) 942, 1506, 1767
Bme18I GGWCC 4 cut(s) 476, 938, 1146, 1457
BmeRI GACNNNNNGTC 1 cut(s) 1280
BmeT110I CYCGRG 1 cut(s) 2078
BmgT120I GGNCC 7 cut(s) 476, 938, 1146, 1220, 1236, 1457, 2114
BmrFI CCNGG 3 cut(s) 942, 1506, 1767
BmrI ACTGGG 3 cut(s) 1217, 1584, 1863
BmsI GCATC 7 cut(s) 454, 641, 757, 784, 1328, 1702, 2153
BmuI ACTGGG 3 cut(s) 1217, 1584, 1863
BpiI GAAGAC 2 cut(s) 307, 1954
BplI GAGNNNNNCTC 2 cut(s) 379, 411
BpmI CTGGAG 2 cut(s) 763, 1064
Bpu10I CCTNAGC 1 cut(s) 78
Bpu14I TTCGAA 1 cut(s) 579
BpuEI CTTGAG 2 cut(s) 592, 1469
BpuMI CCSGG 1 cut(s) 942
BsaAI YACGTR 2 cut(s) 146, 719
BsaJI CCNNGG 3 cut(s) 364, 971, 1020
Bsc4I CCNNNNNNNGG 8 cut(s) 903, 1155, 1538, 1541, 1603, 1753, 1766, 1854
Bse118I RCCGGY 1 cut(s) 1648
Bse1I ACTGG 6 cut(s) 295, 436, 848, 1223, 1579, 1858
Bse3DI GCAATG 3 cut(s) 264, 520, 798
BseBI CCWGG 2 cut(s) 1506, 1767
BseDI CCNNGG 3 cut(s) 364, 971, 1020
BseGI GGATG 3 cut(s) 304, 1633, 2164
BseLI CCNNNNNNNGG 8 cut(s) 903, 1155, 1538, 1541, 1603, 1753, 1766, 1854
BseMI GCAATG 3 cut(s) 264, 520, 798
BseMII CTCAG 2 cut(s) 40, 92
BseNI ACTGG 6 cut(s) 295, 436, 848, 1223, 1579, 1858
BseRI GAGGAG 1 cut(s) 714
BseSI GKGCMC 1 cut(s) 1440
BseX3I CGGCCG 1 cut(s) 1605
BseXI GCAGC 7 cut(s) 61, 241, 653, 976, 1265, 1535, 1639
BseYI CCCAGC 1 cut(s) 644
BsgI GTGCAG 2 cut(s) 783, 1272
Bsh1285I CGRYCG 1 cut(s) 1608
BshFI GGCC 6 cut(s) 442, 1221, 1238, 1607, 1737, 2116
BshNI GGYRCC 3 cut(s) 133, 706, 1501
BsiEI CGRYCG 1 cut(s) 1608
BsiHKAI GWGCWC 1 cut(s) 553
BsiHKCI CYCGRG 1 cut(s) 2078
BsiSI CCGG 2 cut(s) 941, 1649
BslFI GGGAC 4 cut(s) 1068, 1159, 1591, 2046
BslI CCNNNNNNNGG 8 cut(s) 903, 1155, 1538, 1541, 1603, 1753, 1766, 1854
BsmAI GTCTC 6 cut(s) 597, 600, 1107, 1156, 1565, 2143
BsmBI CGTCTC 2 cut(s) 1107, 1156
BsmFI GGGAC 4 cut(s) 1068, 1159, 1591, 2046
BsmI GAATGC 2 cut(s) 785, 1384
BsnI GGCC 6 cut(s) 442, 1221, 1238, 1607, 1737, 2116
BsoBI CYCGRG 1 cut(s) 2078
Bsp119I TTCGAA 1 cut(s) 579
Bsp1286I GDGCHC 4 cut(s) 553, 1440, 1759, 2186
Bsp1407I TGTACA 1 cut(s) 729
Bsp143I GATC 6 cut(s) 148, 333, 1191, 1467, 1780, 1988
Bsp19I CCATGG 1 cut(s) 971
BspACI CCGC 6 cut(s) 39, 630, 782, 924, 1499, 1680
BspANI GGCC 6 cut(s) 442, 1221, 1238, 1607, 1737, 2116
BspCNI CTCAG 2 cut(s) 39, 91
BspMI ACCTGC 1 cut(s) 1124
BspPI GGATC 2 cut(s) 341, 1996
BspQI GCTCTTC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 579
BspT107I GGYRCC 3 cut(s) 133, 706, 1501
BsrDI GCAATG 3 cut(s) 264, 520, 798
BsrFI RCCGGY 1 cut(s) 1648
BsrGI TGTACA 1 cut(s) 729
BsrI ACTGG 6 cut(s) 295, 436, 848, 1223, 1579, 1858
BssAI RCCGGY 1 cut(s) 1648
BssECI CCNNGG 3 cut(s) 364, 971, 1020
BssMI GATC 6 cut(s) 148, 333, 1191, 1467, 1780, 1988
BssNAI GTATAC 1 cut(s) 1618
BssT1I CCWWGG 3 cut(s) 364, 971, 1020
Bst1107I GTATAC 1 cut(s) 1618
Bst2UI CCWGG 2 cut(s) 1506, 1767
Bst6I CTCTTC 2 cut(s) 12, 1401
BstAPI GCANNNNNTGC 1 cut(s) 722
BstAUI TGTACA 1 cut(s) 729
BstBAI YACGTR 2 cut(s) 146, 719
BstBI TTCGAA 1 cut(s) 579
BstC8I GCNNGC 6 cut(s) 667, 756, 1317, 1591, 1735, 1845
BstDEI CTNAG 5 cut(s) 26, 70, 78, 486, 1177
BstDSI CCRYGG 1 cut(s) 971
BstEII GGTNACC 1 cut(s) 1644
BstF5I GGATG 3 cut(s) 304, 1633, 2164
BstKTI GATC 6 cut(s) 151, 336, 1194, 1470, 1783, 1991
BstMAI GTCTC 6 cut(s) 597, 600, 1107, 1156, 1565, 2143
BstMBI GATC 6 cut(s) 148, 333, 1191, 1467, 1780, 1988
BstMCI CGRYCG 1 cut(s) 1608
BstMWI GCNNNNNNNGC 7 cut(s) 36, 209, 638, 666, 722, 1721, 1730
BstNI CCWGG 2 cut(s) 1506, 1767
BstNSI RCATGY 1 cut(s) 1593
BstPI GGTNACC 1 cut(s) 1644
BstSCI CCNGG 3 cut(s) 940, 1504, 1765
BstSFI CTRYAG 2 cut(s) 167, 984
BstSLI GKGCMC 1 cut(s) 1440
BstV1I GCAGC 7 cut(s) 61, 241, 653, 976, 1265, 1535, 1639
BstV2I GAAGAC 2 cut(s) 307, 1954
BstZ17I GTATAC 1 cut(s) 1618
BstZI CGGCCG 1 cut(s) 1605
BsuI GTATCC 3 cut(s) 76, 1553, 1817
BsuRI GGCC 6 cut(s) 442, 1221, 1238, 1607, 1737, 2116
BtgI CCRYGG 1 cut(s) 971
BtsCI GGATG 3 cut(s) 304, 1633, 2164
BtsIMutI CAGTG 3 cut(s) 429, 660, 1834
BveI ACCTGC 1 cut(s) 1124
Cac8I GCNNGC 6 cut(s) 667, 756, 1317, 1591, 1735, 1845
CaiI CAGNNNCTG 1 cut(s) 770
Cfr10I RCCGGY 1 cut(s) 1648
Cfr13I GGNCC 7 cut(s) 476, 938, 1146, 1220, 1236, 1457, 2114
CseI GACGC 1 cut(s) 1124
Csp6I GTAC 3 cut(s) 134, 730, 1777
CviAII CATG 9 cut(s) 155, 206, 354, 617, 890, 919, 972, 1590, 1598
CviQI GTAC 3 cut(s) 134, 730, 1777
DdeI CTNAG 5 cut(s) 26, 70, 78, 486, 1177
DpnI GATC 6 cut(s) 150, 335, 1193, 1469, 1782, 1990
DpnII GATC 6 cut(s) 148, 333, 1191, 1467, 1780, 1988
DriI GACNNNNNGTC 1 cut(s) 1280
EaeI YGGCCR 2 cut(s) 440, 1605
EagI CGGCCG 1 cut(s) 1605
Eam1104I CTCTTC 2 cut(s) 12, 1401
Eam1105I GACNNNNNGTC 1 cut(s) 1280
EarI CTCTTC 2 cut(s) 12, 1401
EclXI CGGCCG 1 cut(s) 1605
Eco130I CCWWGG 3 cut(s) 364, 971, 1020
Eco24I GRGCYC 2 cut(s) 1759, 2186
Eco32I GATATC 1 cut(s) 409
Eco47I GGWCC 4 cut(s) 476, 938, 1146, 1457
Eco52I CGGCCG 1 cut(s) 1605
Eco57I CTGAAG 1 cut(s) 1425
Eco88I CYCGRG 1 cut(s) 2078
Eco91I GGTNACC 1 cut(s) 1644
EcoO65I GGTNACC 1 cut(s) 1644
EcoRI GAATTC 2 cut(s) 843, 1996
EcoRII CCWGG 2 cut(s) 1504, 1765
EcoRV GATATC 1 cut(s) 409
EcoT14I CCWWGG 3 cut(s) 364, 971, 1020
EcoT38I GRGCYC 2 cut(s) 1759, 2186
ErhI CCWWGG 3 cut(s) 364, 971, 1020
Esp3I CGTCTC 2 cut(s) 1107, 1156
FaeI CATG 9 cut(s) 158, 209, 357, 620, 893, 922, 975, 1593, 1601
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FaqI GGGAC 4 cut(s) 1068, 1159, 1591, 2046
FatI CATG 9 cut(s) 154, 205, 353, 616, 889, 918, 971, 1589, 1597
FauNDI CATATG 1 cut(s) 1432
FbaI TGATCA 1 cut(s) 1191
FblI GTMKAC 2 cut(s) 813, 1617
Fnu4HI GCNGC 9 cut(s) 75, 255, 642, 783, 990, 1254, 1500, 1524, 1653
FokI GGATG 3 cut(s) 311, 1620, 2171
FriOI GRGCYC 2 cut(s) 1759, 2186
Fsp4HI GCNGC 9 cut(s) 75, 255, 642, 783, 990, 1254, 1500, 1524, 1653
FspBI CTAG 4 cut(s) 1155, 1704, 1752, 1866
GluI GCNGC 9 cut(s) 75, 255, 642, 783, 990, 1254, 1500, 1524, 1653
GsaI CCCAGC 1 cut(s) 648
GsuI CTGGAG 2 cut(s) 763, 1064
HaeIII GGCC 6 cut(s) 442, 1221, 1238, 1607, 1737, 2116
HapII CCGG 2 cut(s) 941, 1649
HgaI GACGC 1 cut(s) 1124
Hin1II CATG 9 cut(s) 158, 209, 357, 620, 893, 922, 975, 1593, 1601
HincII GTYRAC 2 cut(s) 181, 814
HindII GTYRAC 2 cut(s) 181, 814
HinfI GANTC 8 cut(s) 446, 593, 605, 1264, 1462, 1939, 2034, 2050
HpaII CCGG 2 cut(s) 941, 1649
HphI GGTGA 3 cut(s) 1009, 1638, 1799
Hpy166II GTNNAC 9 cut(s) 181, 264, 730, 814, 1618, 1777, 1790, 1817, 1835
Hpy188I TCNGA 6 cut(s) 451, 548, 1269, 1461, 1467, 1944
Hpy188III TCNNGA 4 cut(s) 80, 374, 398, 2179
Hpy8I GTNNAC 9 cut(s) 181, 264, 730, 814, 1618, 1777, 1790, 1817, 1835
HpyAV CCTTC 4 cut(s) 32, 586, 1758, 1926
HpyCH4IV ACGT 4 cut(s) 145, 174, 718, 1620
HpyF10VI GCNNNNNNNGC 7 cut(s) 36, 209, 638, 666, 722, 1721, 1730
HpyF3I CTNAG 5 cut(s) 26, 70, 78, 486, 1177
HpySE526I ACGT 4 cut(s) 145, 174, 718, 1620
Hsp92II CATG 9 cut(s) 158, 209, 357, 620, 893, 922, 975, 1593, 1601
KpnI GGTACC 1 cut(s) 137
Ksp22I TGATCA 1 cut(s) 1191
Kzo9I GATC 6 cut(s) 148, 333, 1191, 1467, 1780, 1988
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 7 cut(s) 548, 744, 944, 1432, 1730, 1762, 2029
Lsp1109I GCAGC 7 cut(s) 61, 241, 653, 976, 1265, 1535, 1639
LweI GCATC 7 cut(s) 454, 641, 757, 784, 1328, 1702, 2153
MaeI CTAG 4 cut(s) 1155, 1704, 1752, 1866
MaeII ACGT 4 cut(s) 145, 174, 718, 1620
MaeIII GTNAC 7 cut(s) 61, 530, 955, 1034, 1644, 1975, 2101
MalI GATC 6 cut(s) 150, 335, 1193, 1469, 1782, 1990
MboI GATC 6 cut(s) 148, 333, 1191, 1467, 1780, 1988
MfeI CAATTG 2 cut(s) 876, 1028
MhlI GDGCHC 4 cut(s) 553, 1440, 1759, 2186
MlsI TGGCCA 1 cut(s) 442
MluNI TGGCCA 1 cut(s) 442
MlyI GAGTC 4 cut(s) 455, 599, 602, 2028
MmeI TCCRAC 2 cut(s) 1314, 1966
MnlI CCTC 9 cut(s) 41, 489, 692, 695, 928, 1045, 1088, 1308, 1450
Mox20I TGGCCA 1 cut(s) 442
MscI TGGCCA 1 cut(s) 442
MseI TTAA 9 cut(s) 384, 459, 1100, 1140, 1370, 1413, 1935, 2013, 2209
MslI CAYNNNNRTG 1 cut(s) 1022
Msp20I TGGCCA 1 cut(s) 442
MspA1I CMGCKG 2 cut(s) 644, 926
MspI CCGG 2 cut(s) 941, 1649
MspR9I CCNGG 3 cut(s) 942, 1506, 1767
MunI CAATTG 2 cut(s) 876, 1028
Mva1269I GAATGC 2 cut(s) 785, 1384
MvaI CCWGG 2 cut(s) 1506, 1767
MwoI GCNNNNNNNGC 7 cut(s) 36, 209, 638, 666, 722, 1721, 1730
NciI CCSGG 1 cut(s) 942
NcoI CCATGG 1 cut(s) 971
NdeI CATATG 1 cut(s) 1432
NdeII GATC 6 cut(s) 148, 333, 1191, 1467, 1780, 1988
NlaIII CATG 9 cut(s) 158, 209, 357, 620, 893, 922, 975, 1593, 1601
NmuCI GTSAC 4 cut(s) 955, 1644, 1975, 2101
NspI RCATGY 1 cut(s) 1593
NspV TTCGAA 1 cut(s) 579
OliI CACNNNNGTG 1 cut(s) 1022
PaeI GCATGC 1 cut(s) 1593
PciSI GCTCTTC 1 cut(s) 12
PctI GAATGC 2 cut(s) 785, 1384
PfeI GAWTC 4 cut(s) 1264, 1462, 1939, 2050
PflFI GACNNNGTC 1 cut(s) 809
PflMI CCANNNNNTGG 2 cut(s) 1541, 1766
PfoI TCCNGGA 1 cut(s) 940
PkrI GCNGC 9 cut(s) 76, 256, 643, 784, 991, 1255, 1501, 1525, 1654
PleI GAGTC 4 cut(s) 454, 599, 601, 2028
PpsI GAGTC 4 cut(s) 454, 599, 601, 2028
Ppu21I YACGTR 2 cut(s) 146, 719
PshBI ATTAAT 1 cut(s) 1413
Psp6I CCWGG 2 cut(s) 1504, 1765
PspEI GGTNACC 1 cut(s) 1644
PspFI CCCAGC 1 cut(s) 644
PspGI CCWGG 2 cut(s) 1504, 1765
PspPI GGNCC 7 cut(s) 476, 938, 1146, 1220, 1236, 1457, 2114
PstNI CAGNNNCTG 1 cut(s) 770
PsyI GACNNNGTC 1 cut(s) 809
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 3 cut(s) 135, 731, 1778
RsaNI GTAC 3 cut(s) 134, 730, 1777
RseI CAYNNNNRTG 1 cut(s) 1022
SalI GTCGAC 1 cut(s) 812
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 9 cut(s) 384, 459, 1100, 1140, 1370, 1413, 1935, 2013, 2209
SatI GCNGC 9 cut(s) 75, 255, 642, 783, 990, 1254, 1500, 1524, 1653
Sau3AI GATC 6 cut(s) 148, 333, 1191, 1467, 1780, 1988
Sau96I GGNCC 7 cut(s) 476, 938, 1146, 1220, 1236, 1457, 2114
SchI GAGTC 4 cut(s) 455, 599, 602, 2028
ScrFI CCNGG 3 cut(s) 942, 1506, 1767
SduI GDGCHC 4 cut(s) 553, 1440, 1759, 2186
SfaNI GCATC 7 cut(s) 454, 641, 757, 784, 1328, 1702, 2153
SfcI CTRYAG 2 cut(s) 167, 984
SfuI TTCGAA 1 cut(s) 579
SinI GGWCC 4 cut(s) 476, 938, 1146, 1457
SmiMI CAYNNNNRTG 1 cut(s) 1022
SmlI CTYRAG 2 cut(s) 607, 1484
SmoI CTYRAG 2 cut(s) 607, 1484
SpeI ACTAGT 1 cut(s) 1865
SphI GCATGC 1 cut(s) 1593
SsiI CCGC 6 cut(s) 39, 630, 782, 924, 1499, 1680
SspMI CTAG 4 cut(s) 1155, 1704, 1752, 1866
StyD4I CCNGG 3 cut(s) 940, 1504, 1765
StyI CCWWGG 3 cut(s) 364, 971, 1020
TaiI ACGT 4 cut(s) 148, 177, 721, 1623
TaqI TCGA 6 cut(s) 10, 57, 579, 789, 813, 1994
TatI WGTACW 1 cut(s) 729
TauI GCSGC 2 cut(s) 785, 1502
TfiI GAWTC 4 cut(s) 1264, 1462, 1939, 2050
Tru1I TTAA 9 cut(s) 384, 459, 1100, 1140, 1370, 1413, 1935, 2013, 2209
Tru9I TTAA 9 cut(s) 384, 459, 1100, 1140, 1370, 1413, 1935, 2013, 2209
TscAI CASTG 3 cut(s) 436, 660, 1834
TseFI GTSAC 4 cut(s) 955, 1644, 1975, 2101
TseI GCWGC 7 cut(s) 74, 254, 641, 989, 1253, 1523, 1652
Tsp45I GTSAC 4 cut(s) 955, 1644, 1975, 2101
TspDTI ATGAA 8 cut(s) 143, 222, 858, 878, 1003, 1305, 1622, 1863
TspGWI ACGGA 1 cut(s) 1743
TspRI CASTG 3 cut(s) 436, 660, 1834
Tth111I GACNNNGTC 1 cut(s) 809
Van91I CCANNNNNTGG 2 cut(s) 1541, 1766
VpaK11BI GGWCC 4 cut(s) 476, 938, 1146, 1457
VspI ATTAAT 1 cut(s) 1413
XapI RAATTY 7 cut(s) 435, 843, 1088, 1718, 1946, 1996, 2131
XceI RCATGY 1 cut(s) 1593
XcmI CCANNNNNNNNNTGG 2 cut(s) 489, 1027
XmiI GTMKAC 2 cut(s) 813, 1617
XspI CTAG 4 cut(s) 1155, 1704, 1752, 1866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.