Rh1AG072300

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000390
Physical Location & Seq
Reverse (-)
12143217 .. 12143738
522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG072300.1

Sequence Viewer

Length: 522 bp
ATGAGTGCCAAAACTAGCTCTGATGCTGAATTTGCATACGGAGCTGGCCTGATAAACCCTTCTAGGGCTCCATATCCTGGTTTGGTGTACGATCTTGATGAACAAGACTACATAGATTTTTTGTGTTCACAAGGATACAATGGTAAACTATTGAAAGCCATAACCAAGGACAAGACTAGTTGTTCATCAAAATCTAATAATGGAACAACCAATGACCTGAACTATCCTTCTTTTGCACTTTCCATCAAGGATCCAAAATTTGTTAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCGTTGAATTCCACATACAGAGTTAAAGTGGTGGCTCCATTGGGACTCAAAATCAATGTGAATCCAAATGTGTTATCGTTCACATCTCTCGGGCAAAAGAAATCTTTTGTTGTCACTATAAAAGGGCCGATTGAGAAATCAAATTTAGTCTCTGCGTCTTTGATGTGGGATGATGGTGTTTTCCAAGTCAGGAGCCCCATCGTTGTCTATGTTGCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

173

Amino Acids

18.75

Weight (kDa)

9.17

Isoelectric Point (pI)

19.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_6 PF17766 72 - 169 2.2e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 77
AclWI GGATC 3 cut(s) 245, 258, 307
AcsI RAATTY 4 cut(s) 29, 257, 307, 442
AfaI GTAC 1 cut(s) 89
AfiI CCNNNNNNNGG 2 cut(s) 64, 77
AgsI TTSAA 2 cut(s) 154, 307
AhlI ACTAGT 1 cut(s) 176
AjnI CCWGG 1 cut(s) 76
AluBI AGCT 2 cut(s) 18, 44
AluI AGCT 2 cut(s) 18, 44
Alw26I GTCTC 1 cut(s) 454
AlwI GGATC 3 cut(s) 245, 258, 307
Ama87I CYCGRG 1 cut(s) 389
AoxI GGCC 2 cut(s) 46, 425
ApoI RAATTY 4 cut(s) 29, 257, 307, 442
AspS9I GGNCC 1 cut(s) 425
AvaI CYCGRG 1 cut(s) 389
BaeI ACNNNNGTAYC 2 cut(s) 127, 160
BamHI GGATCC 1 cut(s) 250
BanII GRGCYC 2 cut(s) 70, 497
BbsI GAAGAC 1 cut(s) 265
BccI CCATC 3 cut(s) 251, 467, 506
BciT130I CCWGG 1 cut(s) 78
BciVI GTATCC 1 cut(s) 128
BcoDI GTCTC 1 cut(s) 454
BcuI ACTAGT 1 cut(s) 176
BfaI CTAG 3 cut(s) 15, 63, 177
BfuI GTATCC 1 cut(s) 128
Bme1390I CCNGG 1 cut(s) 78
BmeT110I CYCGRG 1 cut(s) 389
BmgT120I GGNCC 1 cut(s) 425
BmiI GGNNCC 4 cut(s) 69, 252, 336, 494
BmrFI CCNGG 1 cut(s) 78
BmsI GCATC 1 cut(s) 13
BpiI GAAGAC 1 cut(s) 265
BsaJI CCNNGG 1 cut(s) 165
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 77
BseBI CCWGG 1 cut(s) 78
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 1 cut(s) 475
BseLI CCNNNNNNNGG 2 cut(s) 64, 77
BshFI GGCC 2 cut(s) 48, 427
BsiHKCI CYCGRG 1 cut(s) 389
BslFI GGGAC 1 cut(s) 357
BslI CCNNNNNNNGG 2 cut(s) 64, 77
BsmAI GTCTC 1 cut(s) 454
BsmFI GGGAC 1 cut(s) 357
BsnI GGCC 2 cut(s) 48, 427
BsoBI CYCGRG 1 cut(s) 389
Bsp1286I GDGCHC 2 cut(s) 70, 497
Bsp143I GATC 3 cut(s) 91, 250, 299
BspANI GGCC 2 cut(s) 48, 427
BspLI GGNNCC 4 cut(s) 69, 252, 336, 494
BspPI GGATC 3 cut(s) 245, 258, 307
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 3 cut(s) 91, 250, 299
BssT1I CCWWGG 1 cut(s) 165
Bst2UI CCWGG 1 cut(s) 78
Bst4CI ACNGT 1 cut(s) 286
BstC8I GCNNGC 1 cut(s) 46
BstF5I GGATG 1 cut(s) 475
BstKTI GATC 3 cut(s) 94, 253, 302
BstMAI GTCTC 1 cut(s) 454
BstMBI GATC 3 cut(s) 91, 250, 299
BstMWI GCNNNNNNNGC 2 cut(s) 32, 41
BstNI CCWGG 1 cut(s) 78
BstSCI CCNGG 1 cut(s) 76
BstV2I GAAGAC 1 cut(s) 265
BstX2I RGATCY 1 cut(s) 250
BstYI RGATCY 1 cut(s) 250
BsuI GTATCC 1 cut(s) 128
BsuRI GGCC 2 cut(s) 48, 427
BtsCI GGATG 1 cut(s) 475
Cac8I GCNNGC 1 cut(s) 46
Cfr13I GGNCC 1 cut(s) 425
CseI GACGC 1 cut(s) 444
Csp6I GTAC 1 cut(s) 88
CviJI RGCY 8 cut(s) 18, 44, 48, 68, 158, 335, 427, 495
CviKI_1 RGCY 8 cut(s) 18, 44, 48, 68, 158, 335, 427, 495
CviQI GTAC 1 cut(s) 88
DpnI GATC 3 cut(s) 93, 252, 301
DpnII GATC 3 cut(s) 91, 250, 299
Eco130I CCWWGG 1 cut(s) 165
Eco24I GRGCYC 2 cut(s) 70, 497
Eco88I CYCGRG 1 cut(s) 389
EcoRI GAATTC 1 cut(s) 307
EcoRII CCWGG 1 cut(s) 76
EcoT14I CCWWGG 1 cut(s) 165
EcoT38I GRGCYC 2 cut(s) 70, 497
ErhI CCWWGG 1 cut(s) 165
FaiI YATR 8 cut(s) 37, 73, 113, 161, 279, 316, 419, 510
FaqI GGGAC 1 cut(s) 357
FokI GGATG 1 cut(s) 482
FriOI GRGCYC 2 cut(s) 70, 497
FspBI CTAG 3 cut(s) 15, 63, 177
HaeIII GGCC 2 cut(s) 48, 427
HgaI GACGC 1 cut(s) 444
HinfI GANTC 2 cut(s) 345, 361
Hpy166II GTNNAC 4 cut(s) 88, 128, 146, 381
Hpy188I TCNGA 1 cut(s) 22
Hpy188III TCNNGA 2 cut(s) 95, 490
Hpy8I GTNNAC 4 cut(s) 88, 128, 146, 381
HpyAV CCTTC 2 cut(s) 69, 237
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4V TGCA 3 cut(s) 35, 236, 515
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 41
Kzo9I GATC 3 cut(s) 91, 250, 299
LmnI GCTCC 4 cut(s) 41, 73, 340, 492
LpnPI CCDG 6 cut(s) 30, 62, 63, 90, 230, 475
LweI GCATC 1 cut(s) 13
MaeI CTAG 3 cut(s) 15, 63, 177
MaeIII GTNAC 2 cut(s) 286, 412
MalI GATC 3 cut(s) 93, 252, 301
MboI GATC 3 cut(s) 91, 250, 299
MboII GAAGA 1 cut(s) 265
MflI RGATCY 1 cut(s) 250
MhlI GDGCHC 2 cut(s) 70, 497
MluCI AATT 4 cut(s) 29, 257, 307, 442
MlyI GAGTC 1 cut(s) 339
MmeI TCCRAC 1 cut(s) 277
MseI TTAA 3 cut(s) 264, 324, 520
MspR9I CCNGG 1 cut(s) 78
MvaI CCWGG 1 cut(s) 78
MwoI GCNNNNNNNGC 2 cut(s) 32, 41
NdeII GATC 3 cut(s) 91, 250, 299
NlaIV GGNNCC 4 cut(s) 69, 252, 336, 494
NmuCI GTSAC 2 cut(s) 286, 412
PfeI GAWTC 1 cut(s) 361
PflMI CCANNNNNTGG 1 cut(s) 77
PleI GAGTC 1 cut(s) 339
PpsI GAGTC 1 cut(s) 339
Psp6I CCWGG 1 cut(s) 76
PspGI CCWGG 1 cut(s) 76
PspN4I GGNNCC 4 cut(s) 69, 252, 336, 494
PspPI GGNCC 1 cut(s) 425
PsuI RGATCY 1 cut(s) 250
RsaI GTAC 1 cut(s) 89
RsaNI GTAC 1 cut(s) 88
SaqAI TTAA 3 cut(s) 264, 324, 520
Sau3AI GATC 3 cut(s) 91, 250, 299
Sau96I GGNCC 1 cut(s) 425
SchI GAGTC 1 cut(s) 339
ScrFI CCNGG 1 cut(s) 78
SduI GDGCHC 2 cut(s) 70, 497
SetI ASST 3 cut(s) 20, 46, 219
SfaNI GCATC 1 cut(s) 13
SpeI ACTAGT 1 cut(s) 176
Sse9I AATT 4 cut(s) 29, 257, 307, 442
SspMI CTAG 3 cut(s) 15, 63, 177
StyD4I CCNGG 1 cut(s) 76
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 1 cut(s) 286
TasI AATT 4 cut(s) 29, 257, 307, 442
TfiI GAWTC 1 cut(s) 361
Tru1I TTAA 3 cut(s) 264, 324, 520
Tru9I TTAA 3 cut(s) 264, 324, 520
TseFI GTSAC 2 cut(s) 286, 412
Tsp45I GTSAC 2 cut(s) 286, 412
TspDTI ATGAA 2 cut(s) 114, 174
TspGWI ACGGA 1 cut(s) 54
Van91I CCANNNNNTGG 1 cut(s) 77
XapI RAATTY 4 cut(s) 29, 257, 307, 442
XspI CTAG 3 cut(s) 15, 63, 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.