RchiOBHm_Chr1g0344301

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000390
Physical Location & Seq
Reverse (-)
36610790 .. 36614089
3300 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57076

Sequence Viewer

Length: 2229 bp
ATGGCTCTTCGATGGTTTCTCCTTCTCAGCCTCATTAGCAGTATTCTACTTCTTGTTCATGTTACTCACTCAGCTGCTCGGGATGCCCGGAAGGCTTATATTGTGTATATGGGCGACAAGCCAAAGAACGGGGTGCCAGTAACACCTGATCTTCATGTGAATATTCTACGAGACGTAGTTGACAACAACATTAATAATGATATTGCACATGAAGCTCTGCTTCTTCACAGCTACAAGAGAAGTTTCCATGGATTTGCTGCAATGCTCACAAAACAAGAAGCACAAAAACTGGCTGGAATGGATGGTGTAGTATCTGTCTTCCCAAGCAAACAAATGAGCGTCAAAACGACAAAGTCATGGAACTTCCTTGAGTTTCCAGAAACAGTTAAAAGAAGCGACCTTGAAAAGGATATCATCATCGGTGTGATTGACAGTGGAATTTGGCCAGAATCGGACAGCTTTAGTGATGTTGGTTTTGGTCCTCCACCCAAGAAATGGAAAGGCATATGTCAAGGCAATAACAACTTTACTTGTAACAATAAAATTATCGGAGCACGGTATTACCGTCATTTGGGATCCTTCGAAAAAGAAGTTGAGTCTCCAAGAGACTCTGAAGGTCATGGAACCCATTGCGCATCTGTAGCAGCTGGGAACATAGTGAAAAACACGAGCATAAAAGGTTTAGGGTTTGGAAACGCAAGAGGAGGGGTGCCATCAGCACATATTGCAGTGTACAAAGTGTGTTGGAAGGATGGTTGTTCAGATGCTGACATGCTAGCGGCATTTGATGATGCAGTTGCTGATGGTGTCGACATAATCTCTATTTCCATTGGAAGCAAAGTTCCAAATGATTATTTTCGAAACGGAGTTGCAATTGGGGCATTTCACGCTACCAGAAATGGGATACTTGTTTTAACGGCCAGTGGTAATGAAGGTCCGAAAAAGAAAACTGTGTCAAACTTTGCACCATGGATGCTTTCTGTGGCTGCTACTACCATAAACCGTCAGTTCATCACCAAGGTTCAATTAGGTAACGGTAAAATCTATGAGGGACTTTTACCAAACATATATGACCTCCGGGGTAAATTCTATCCTTTAATATATGGCGGAGACGCACCTAACACAACAAAAGGTTCTAACGCATCTATGTCCAGGTTCTGTTCCGAAAATAGCTTAAAAGAAGATTCGATCAGAGATAAAATTGTGCTTTGTGATAGAGGCCCTGCTGGCAATGACGATGGGTATGGGGCCATACAGGGTGATGCAACCGGTGTTATTCTGACAGGCGTAAAAATTGCCGAGGAGCTGGTTAGCCCTCTTAGCCTGCCTGCATCTCACATCGGATTGGAAGAAAACAGCCACATTTACAAATACATAAGCGAAACAAGGAACCCAATTGCAACTATTTTCAAAAGTGAAGAGATTAATGATGTATTGGCTCCATACGTGCCCTCCTACTCGTCAAGGGGTCCAAATCCAATGAATCCTAACATTCTCAAGCCAGATTTAGCAGCCCCAGGTACTCACATTTTAGCAGCATATGATCATCAATCGGTTGGTGGTGGCAGTAGAGCTAAGTATAATTTGGAGACTGGGACATCAATGGCATGCCCCCATGTTGCAGGCGTAGCTGCATACGTCAAATCATTTCACTCTAAATGGTCACCGGCTGCTATTCAATCGGCTCTCATCACTACAGCTAAACCTATGAGTACTAAAACTAGCCCACACGCTGAATTTGCATATGGAGCTGGCCTACTAAACCCTTCTAGGGCTCCATATCCTGGTTTAGTATACGATCTTGATGAACAAGACTACTTATATTTTTTGTGTTCACAAGGATATAGTGGAAAACTATTGGAAATCATAACCAGGGACAAGAGTAGCTGCTCATCAAAATCTAATAATGAAACAACCAATGACCTGAACTATCCTTCTTTTGCTCTTTCAATCAAGGATCCAGAATTCATCAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCGTCGAATTCCACATACAGAGCTAAAGTGGTGGCTCCATCAGGACTCGAAATCAATGTGAATCCAAGTGTGCTATCATTTACATCTCTCGGGCAGAAGGAATCTTTTGTTGTTACAGTAAAAGGGTCAATTGAGAAATCAAATATAGTCTCTGCATCTTTGGTGTGGGATGATGGTACTTTCCAAGTCAGGAGCCCGATTGTTATCCATGTTACAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

742

Amino Acids

80.18

Weight (kDa)

7.91

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 113 1.2e-14 Peptidase inhibitor I9
Peptidase_S8 PF00082 136 - 585 4.4e-46 Subtilase family
fn3_6 PF17766 641 - 738 3.7e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 634
AccB1I GGYRCC 2 cut(s) 133, 709
AccB7I CCANNNNNTGG 2 cut(s) 495, 1784
AccI GTMKAC 2 cut(s) 810, 1794
AciI CCGC 2 cut(s) 779, 1107
AclWI GGATC 5 cut(s) 570, 583, 1952, 1965, 2014
AcoI YGGCCR 2 cut(s) 443, 918
AcsI RAATTY 5 cut(s) 438, 1085, 1736, 1964, 2014
AcuI CTGAAG 1 cut(s) 633
AfaI GTAC 4 cut(s) 734, 1522, 1714, 2185
AfiI CCNNNNNNNGG 7 cut(s) 128, 406, 495, 571, 900, 1771, 1784
AgeI ACCGGT 1 cut(s) 1268
AgsI TTSAA 5 cut(s) 404, 1025, 1411, 1679, 1950
AjnI CCWGG 4 cut(s) 1151, 1516, 1783, 1871
AloI GAACNNNNNNTCC 2 cut(s) 825, 857
Alw21I GWGCWC 1 cut(s) 556
Alw26I GTCTC 6 cut(s) 165, 600, 603, 1104, 1583, 2161
AlwI GGATC 5 cut(s) 570, 583, 1952, 1965, 2014
AlwNI CAGNNNCTG 3 cut(s) 767, 800, 1158
Ama87I CYCGRG 2 cut(s) 78, 2096
AoxI GGCC 5 cut(s) 443, 918, 1219, 1248, 1753
ApeKI GCWGC 9 cut(s) 74, 257, 644, 986, 1511, 1535, 1631, 1670, 1887
ApoI RAATTY 5 cut(s) 438, 1085, 1736, 1964, 2014
AseI ATTAAT 2 cut(s) 192, 1425
AsiGI ACCGGT 1 cut(s) 1268
AspLEI GCGC 1 cut(s) 635
AspS9I GGNCC 5 cut(s) 479, 935, 1220, 1248, 1469
AsuC2I CCSGG 2 cut(s) 88, 1079
AsuHPI GGTGA 3 cut(s) 1006, 1271, 1656
AsuII TTCGAA 2 cut(s) 582, 859
AsuNHI GCTAGC 1 cut(s) 775
AvaI CYCGRG 2 cut(s) 78, 2096
AvaII GGWCC 3 cut(s) 479, 935, 1469
BaeGI GKGCMC 1 cut(s) 1452
BalI TGGCCA 1 cut(s) 445
BamHI GGATCC 2 cut(s) 575, 1957
BanI GGYRCC 2 cut(s) 133, 709
BanII GRGCYC 2 cut(s) 1777, 2204
BauI CACGAG 1 cut(s) 667
BbsI GAAGAC 2 cut(s) 310, 1972
Bbv12I GWGCWC 1 cut(s) 556
BbvI GCAGC 9 cut(s) 61, 244, 656, 973, 1523, 1547, 1618, 1657, 1874
BccI CCATC 8 cut(s) 6, 296, 721, 746, 797, 1232, 2053, 2174
BceAI ACGGC 1 cut(s) 933
BciT130I CCWGG 4 cut(s) 1153, 1518, 1785, 1873
BciVI GTATCC 1 cut(s) 897
BclI TGATCA 1 cut(s) 1543
BcnI CCSGG 2 cut(s) 88, 1079
BcoDI GTCTC 6 cut(s) 165, 600, 603, 1104, 1583, 2161
BfaI CTAG 3 cut(s) 776, 1722, 1770
BfmI CTRYAG 2 cut(s) 639, 1695
BfuI GTATCC 1 cut(s) 897
BglI GCCNNNNNGGC 2 cut(s) 92, 1227
BmcAI AGTACT 1 cut(s) 1714
Bme1390I CCNGG 6 cut(s) 88, 1079, 1153, 1518, 1785, 1873
Bme18I GGWCC 3 cut(s) 479, 935, 1469
BmeT110I CYCGRG 2 cut(s) 78, 2096
BmgT120I GGNCC 5 cut(s) 479, 935, 1220, 1248, 1469
BmrFI CCNGG 6 cut(s) 88, 1079, 1153, 1518, 1785, 1873
BmrI ACTGGG 1 cut(s) 1602
BmsI GCATC 9 cut(s) 73, 644, 754, 781, 963, 1151, 1252, 1340, 2171
BmtI GCTAGC 1 cut(s) 779
BmuI ACTGGG 1 cut(s) 1602
BpiI GAAGAC 2 cut(s) 310, 1972
Bpu14I TTCGAA 2 cut(s) 582, 859
BpuEI CTTGAG 2 cut(s) 389, 1481
BpuMI CCSGG 2 cut(s) 88, 1079
BsaAI YACGTR 1 cut(s) 1447
BsaBI GATNNNNATC 1 cut(s) 2210
BsaJI CCNNGG 7 cut(s) 247, 968, 1017, 1078, 1299, 1516, 1872
BsaWI WCCGGW 1 cut(s) 1268
BsaXI ACNNNNNCTCC 2 cut(s) 1436, 1466
Bsc4I CCNNNNNNNGG 7 cut(s) 128, 406, 495, 571, 900, 1771, 1784
Bse118I RCCGGY 2 cut(s) 1268, 1666
Bse1I ACTGG 4 cut(s) 137, 294, 921, 1597
Bse3DI GCAATG 3 cut(s) 267, 628, 1237
Bse8I GATNNNNATC 1 cut(s) 2210
BseBI CCWGG 4 cut(s) 1153, 1518, 1785, 1873
BseDI CCNNGG 7 cut(s) 247, 968, 1017, 1078, 1299, 1516, 1872
BseGI GGATG 5 cut(s) 88, 307, 757, 978, 2182
BseJI GATNNNNATC 1 cut(s) 2210
BseLI CCNNNNNNNGG 7 cut(s) 128, 406, 495, 571, 900, 1771, 1784
BseMI GCAATG 3 cut(s) 267, 628, 1237
BseMII CTCAG 2 cut(s) 40, 84
BseNI ACTGG 4 cut(s) 137, 294, 921, 1597
BseRI GAGGAG 2 cut(s) 717, 1316
BseSI GKGCMC 1 cut(s) 1452
BseXI GCAGC 9 cut(s) 61, 244, 656, 973, 1523, 1547, 1618, 1657, 1874
BseYI CCCAGC 1 cut(s) 647
BshFI GGCC 5 cut(s) 445, 920, 1221, 1250, 1755
BshNI GGYRCC 2 cut(s) 133, 709
BshTI ACCGGT 1 cut(s) 1268
BsiHKAI GWGCWC 1 cut(s) 556
BsiHKCI CYCGRG 2 cut(s) 78, 2096
BsiSI CCGG 4 cut(s) 88, 1078, 1269, 1667
BslFI GGGAC 3 cut(s) 1065, 1609, 1889
BslI CCNNNNNNNGG 7 cut(s) 128, 406, 495, 571, 900, 1771, 1784
BsmAI GTCTC 6 cut(s) 165, 600, 603, 1104, 1583, 2161
BsmBI CGTCTC 2 cut(s) 165, 1104
BsmFI GGGAC 3 cut(s) 1065, 1609, 1889
BsnI GGCC 5 cut(s) 445, 920, 1221, 1250, 1755
BsoBI CYCGRG 2 cut(s) 78, 2096
Bsp119I TTCGAA 2 cut(s) 582, 859
Bsp1286I GDGCHC 4 cut(s) 556, 1452, 1777, 2204
Bsp1407I TGTACA 1 cut(s) 732
Bsp143I GATC 7 cut(s) 148, 575, 1188, 1543, 1798, 1957, 2006
Bsp19I CCATGG 2 cut(s) 247, 968
BspACI CCGC 2 cut(s) 779, 1107
BspANI GGCC 5 cut(s) 445, 920, 1221, 1250, 1755
BspCNI CTCAG 2 cut(s) 39, 83
BspOI GCTAGC 1 cut(s) 779
BspPI GGATC 5 cut(s) 570, 583, 1952, 1965, 2014
BspQI GCTCTTC 1 cut(s) 12
BspT104I TTCGAA 2 cut(s) 582, 859
BspT107I GGYRCC 2 cut(s) 133, 709
BsrDI GCAATG 3 cut(s) 267, 628, 1237
BsrFI RCCGGY 2 cut(s) 1268, 1666
BsrGI TGTACA 1 cut(s) 732
BsrI ACTGG 4 cut(s) 137, 294, 921, 1597
BssAI RCCGGY 2 cut(s) 1268, 1666
BssECI CCNNGG 7 cut(s) 247, 968, 1017, 1078, 1299, 1516, 1872
BssMI GATC 7 cut(s) 148, 575, 1188, 1543, 1798, 1957, 2006
BssNAI GTATAC 1 cut(s) 1795
BssSI CACGAG 1 cut(s) 667
BssT1I CCWWGG 3 cut(s) 247, 968, 1017
Bst1107I GTATAC 1 cut(s) 1795
Bst2BI CACGAG 1 cut(s) 667
Bst2UI CCWGG 4 cut(s) 1153, 1518, 1785, 1873
Bst6I CTCTTC 2 cut(s) 12, 1413
BstAPI GCANNNNNTGC 1 cut(s) 725
BstAUI TGTACA 1 cut(s) 732
BstBAI YACGTR 1 cut(s) 1447
BstBI TTCGAA 2 cut(s) 582, 859
BstC8I GCNNGC 7 cut(s) 777, 1228, 1325, 1329, 1609, 1624, 1753
BstDEI CTNAG 4 cut(s) 26, 70, 1319, 1575
BstDSI CCRYGG 2 cut(s) 247, 968
BstEII GGTNACC 1 cut(s) 1662
BstENI CCTNNNNNAGG 1 cut(s) 404
BstF5I GGATG 5 cut(s) 88, 307, 757, 978, 2182
BstHHI GCGC 1 cut(s) 635
BstKTI GATC 7 cut(s) 151, 578, 1191, 1546, 1801, 1960, 2009
BstMAI GTCTC 6 cut(s) 165, 600, 603, 1104, 1583, 2161
BstMBI GATC 7 cut(s) 148, 575, 1188, 1543, 1798, 1957, 2006
BstNI CCWGG 4 cut(s) 1153, 1518, 1785, 1873
BstNSI RCATGY 2 cut(s) 775, 1611
BstPI GGTNACC 1 cut(s) 1662
BstSCI CCNGG 6 cut(s) 86, 1077, 1151, 1516, 1783, 1871
BstSFI CTRYAG 2 cut(s) 639, 1695
BstSLI GKGCMC 1 cut(s) 1452
BstV1I GCAGC 9 cut(s) 61, 244, 656, 973, 1523, 1547, 1618, 1657, 1874
BstV2I GAAGAC 2 cut(s) 310, 1972
BstX2I RGATCY 2 cut(s) 575, 1957
BstYI RGATCY 2 cut(s) 575, 1957
BstZ17I GTATAC 1 cut(s) 1795
BsuI GTATCC 1 cut(s) 897
BsuRI GGCC 5 cut(s) 445, 920, 1221, 1250, 1755
BtgI CCRYGG 2 cut(s) 247, 968
BtsCI GGATG 5 cut(s) 88, 307, 757, 978, 2182
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 3 cut(s) 439, 735, 928
Cac8I GCNNGC 7 cut(s) 777, 1228, 1325, 1329, 1609, 1624, 1753
CaiI CAGNNNCTG 3 cut(s) 767, 800, 1158
CfoI GCGC 1 cut(s) 635
Cfr10I RCCGGY 2 cut(s) 1268, 1666
Cfr13I GGNCC 5 cut(s) 479, 935, 1220, 1248, 1469
CseI GACGC 2 cut(s) 328, 1121
Csp6I GTAC 4 cut(s) 733, 1521, 1713, 2184
CspAI ACCGGT 1 cut(s) 1268
CviQI GTAC 4 cut(s) 733, 1521, 1713, 2184
DdeI CTNAG 4 cut(s) 26, 70, 1319, 1575
DpnI GATC 7 cut(s) 150, 577, 1190, 1545, 1800, 1959, 2008
DpnII GATC 7 cut(s) 148, 575, 1188, 1543, 1798, 1957, 2006
EaeI YGGCCR 2 cut(s) 443, 918
Eam1104I CTCTTC 2 cut(s) 12, 1413
EarI CTCTTC 2 cut(s) 12, 1413
EciI GGCGGA 1 cut(s) 1122
Eco130I CCWWGG 3 cut(s) 247, 968, 1017
Eco24I GRGCYC 2 cut(s) 1777, 2204
Eco32I GATATC 1 cut(s) 412
Eco47I GGWCC 3 cut(s) 479, 935, 1469
Eco57I CTGAAG 1 cut(s) 633
Eco88I CYCGRG 2 cut(s) 78, 2096
Eco91I GGTNACC 1 cut(s) 1662
EcoNI CCTNNNNNAGG 1 cut(s) 404
EcoO109I RGGNCCY 1 cut(s) 1220
EcoO65I GGTNACC 1 cut(s) 1662
EcoRI GAATTC 2 cut(s) 1964, 2014
EcoRII CCWGG 4 cut(s) 1151, 1516, 1783, 1871
EcoRV GATATC 1 cut(s) 412
EcoT14I CCWWGG 3 cut(s) 247, 968, 1017
EcoT38I GRGCYC 2 cut(s) 1777, 2204
ErhI CCWWGG 3 cut(s) 247, 968, 1017
Esp3I CGTCTC 2 cut(s) 165, 1104
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FaqI GGGAC 3 cut(s) 1065, 1609, 1889
FauNDI CATATG 3 cut(s) 506, 1540, 1744
FbaI TGATCA 1 cut(s) 1543
FblI GTMKAC 2 cut(s) 810, 1794
FokI GGATG 5 cut(s) 95, 314, 764, 985, 2189
FriOI GRGCYC 2 cut(s) 1777, 2204
FspBI CTAG 3 cut(s) 776, 1722, 1770
FspI TGCGCA 1 cut(s) 634
GlaI GCGC 1 cut(s) 634
GsaI CCCAGC 1 cut(s) 651
HaeIII GGCC 5 cut(s) 445, 920, 1221, 1250, 1755
HapII CCGG 4 cut(s) 88, 1078, 1269, 1667
HgaI GACGC 2 cut(s) 328, 1121
HhaI GCGC 1 cut(s) 635
Hin6I GCGC 1 cut(s) 633
HinP1I GCGC 1 cut(s) 633
HincII GTYRAC 2 cut(s) 181, 811
HindII GTYRAC 2 cut(s) 181, 811
HinfI GANTC 8 cut(s) 449, 596, 608, 1184, 1483, 2052, 2068, 2108
HpaII CCGG 4 cut(s) 88, 1078, 1269, 1667
HphI GGTGA 3 cut(s) 1006, 1271, 1656
Hpy166II GTNNAC 5 cut(s) 181, 733, 811, 1795, 1835
Hpy188I TCNGA 9 cut(s) 454, 551, 613, 763, 939, 1165, 1193, 1281, 1343
Hpy188III TCNNGA 6 cut(s) 80, 377, 1802, 1961, 2049, 2197
Hpy8I GTNNAC 5 cut(s) 181, 733, 811, 1795, 1835
Hpy99I CGWCG 1 cut(s) 2014
HpyAV CCTTC 9 cut(s) 32, 85, 589, 608, 742, 926, 1776, 1944, 2098
HpyCH4IV ACGT 3 cut(s) 174, 1446, 1638
HpyF3I CTNAG 4 cut(s) 26, 70, 1319, 1575
HpySE526I ACGT 3 cut(s) 174, 1446, 1638
HspAI GCGC 1 cut(s) 633
Ksp22I TGATCA 1 cut(s) 1543
Kzo9I GATC 7 cut(s) 148, 575, 1188, 1543, 1798, 1957, 2006
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 7 cut(s) 551, 1303, 1444, 1748, 1780, 2047, 2199
Lsp1109I GCAGC 9 cut(s) 61, 244, 656, 973, 1523, 1547, 1618, 1657, 1874
LweI GCATC 9 cut(s) 73, 644, 754, 781, 963, 1151, 1252, 1340, 2171
MaeI CTAG 3 cut(s) 776, 1722, 1770
MaeII ACGT 3 cut(s) 174, 1446, 1638
MaeIII GTNAC 8 cut(s) 61, 139, 533, 1031, 1662, 1993, 2119, 2218
MalI GATC 7 cut(s) 150, 577, 1190, 1545, 1800, 1959, 2008
MboI GATC 7 cut(s) 148, 575, 1188, 1543, 1798, 1957, 2006
MboII GAAGA 7 cut(s) 143, 215, 310, 1193, 1361, 1430, 1972
MfeI CAATTG 3 cut(s) 873, 1395, 2136
MflI RGATCY 2 cut(s) 575, 1957
MhlI GDGCHC 4 cut(s) 556, 1452, 1777, 2204
MlsI TGGCCA 1 cut(s) 445
MluNI TGGCCA 1 cut(s) 445
MlyI GAGTC 3 cut(s) 602, 605, 2046
MmeI TCCRAC 2 cut(s) 725, 1984
Mox20I TGGCCA 1 cut(s) 445
MscI TGGCCA 1 cut(s) 445
MseI TTAA 7 cut(s) 192, 387, 914, 1097, 1175, 1425, 2227
MslI CAYNNNNRTG 1 cut(s) 422
Msp20I TGGCCA 1 cut(s) 445
MspA1I CMGCKG 2 cut(s) 74, 647
MspI CCGG 4 cut(s) 88, 1078, 1269, 1667
MspR9I CCNGG 6 cut(s) 88, 1079, 1153, 1518, 1785, 1873
MunI CAATTG 3 cut(s) 873, 1395, 2136
MvaI CCWGG 4 cut(s) 1153, 1518, 1785, 1873
NciI CCSGG 2 cut(s) 88, 1079
NcoI CCATGG 2 cut(s) 247, 968
NdeI CATATG 3 cut(s) 506, 1540, 1744
NdeII GATC 7 cut(s) 148, 575, 1188, 1543, 1798, 1957, 2006
NheI GCTAGC 1 cut(s) 775
NmeAIII GCCGAG 1 cut(s) 1324
NmuCI GTSAC 2 cut(s) 1662, 1993
NsbI TGCGCA 1 cut(s) 634
NspI RCATGY 2 cut(s) 775, 1611
NspV TTCGAA 2 cut(s) 582, 859
PaeI GCATGC 1 cut(s) 1611
PciSI GCTCTTC 1 cut(s) 12
PcsI WCGNNNNNNNCGW 1 cut(s) 562
PfeI GAWTC 5 cut(s) 449, 1184, 1483, 2068, 2108
PflFI GACNNNGTC 1 cut(s) 352
PflMI CCANNNNNTGG 2 cut(s) 495, 1784
PinAI ACCGGT 1 cut(s) 1268
PleI GAGTC 3 cut(s) 602, 604, 2046
PpsI GAGTC 3 cut(s) 602, 604, 2046
Ppu21I YACGTR 1 cut(s) 1447
PshBI ATTAAT 2 cut(s) 192, 1425
Psp6I CCWGG 4 cut(s) 1151, 1516, 1783, 1871
PspEI GGTNACC 1 cut(s) 1662
PspFI CCCAGC 1 cut(s) 647
PspGI CCWGG 4 cut(s) 1151, 1516, 1783, 1871
PspPI GGNCC 5 cut(s) 479, 935, 1220, 1248, 1469
PstNI CAGNNNCTG 3 cut(s) 767, 800, 1158
PsuI RGATCY 2 cut(s) 575, 1957
PsyI GACNNNGTC 1 cut(s) 352
PvuII CAGCTG 2 cut(s) 74, 647
RsaI GTAC 4 cut(s) 734, 1522, 1714, 2185
RsaNI GTAC 4 cut(s) 733, 1521, 1713, 2184
RseI CAYNNNNRTG 1 cut(s) 422
SalI GTCGAC 1 cut(s) 809
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 7 cut(s) 192, 387, 914, 1097, 1175, 1425, 2227
Sau3AI GATC 7 cut(s) 148, 575, 1188, 1543, 1798, 1957, 2006
Sau96I GGNCC 5 cut(s) 479, 935, 1220, 1248, 1469
ScaI AGTACT 1 cut(s) 1714
SchI GAGTC 3 cut(s) 602, 605, 2046
ScrFI CCNGG 6 cut(s) 88, 1079, 1153, 1518, 1785, 1873
SduI GDGCHC 4 cut(s) 556, 1452, 1777, 2204
SfaNI GCATC 9 cut(s) 73, 644, 754, 781, 963, 1151, 1252, 1340, 2171
SfcI CTRYAG 2 cut(s) 639, 1695
SfuI TTCGAA 2 cut(s) 582, 859
SinI GGWCC 3 cut(s) 479, 935, 1469
SmiMI CAYNNNNRTG 1 cut(s) 422
SmlI CTYRAG 2 cut(s) 368, 1496
SmoI CTYRAG 2 cut(s) 368, 1496
SphI GCATGC 1 cut(s) 1611
SsiI CCGC 2 cut(s) 779, 1107
SspI AATATT 1 cut(s) 163
SspMI CTAG 3 cut(s) 776, 1722, 1770
StyD4I CCNGG 6 cut(s) 86, 1077, 1151, 1516, 1783, 1871
StyI CCWWGG 3 cut(s) 247, 968, 1017
TaiI ACGT 3 cut(s) 177, 1449, 1641
TaqI TCGA 7 cut(s) 10, 582, 810, 859, 1187, 2012, 2055
TatI WGTACW 2 cut(s) 732, 1712
TauI GCSGC 1 cut(s) 782
TfiI GAWTC 5 cut(s) 449, 1184, 1483, 2068, 2108
Tru1I TTAA 7 cut(s) 192, 387, 914, 1097, 1175, 1425, 2227
Tru9I TTAA 7 cut(s) 192, 387, 914, 1097, 1175, 1425, 2227
TscAI CASTG 3 cut(s) 439, 735, 928
TseFI GTSAC 2 cut(s) 1662, 1993
TseI GCWGC 9 cut(s) 74, 257, 644, 986, 1511, 1535, 1631, 1670, 1887
Tsp45I GTSAC 2 cut(s) 1662, 1993
TspDTI ATGAA 9 cut(s) 47, 143, 225, 945, 1000, 1496, 1821, 1923, 1957
TspGWI ACGGA 1 cut(s) 879
TspRI CASTG 3 cut(s) 439, 735, 928
Tth111I GACNNNGTC 1 cut(s) 352
Van91I CCANNNNNTGG 2 cut(s) 495, 1784
VpaK11BI GGWCC 3 cut(s) 479, 935, 1469
VspI ATTAAT 2 cut(s) 192, 1425
XagI CCTNNNNNAGG 1 cut(s) 404
XapI RAATTY 5 cut(s) 438, 1085, 1736, 1964, 2014
XceI RCATGY 2 cut(s) 775, 1611
XcmI CCANNNNNNNNNTGG 1 cut(s) 492
XmiI GTMKAC 2 cut(s) 810, 1794
XspI CTAG 3 cut(s) 776, 1722, 1770
ZrmI AGTACT 1 cut(s) 1714
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.