MD07G1206500.v1.1

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000390
Physical Location & Seq
Forward (+)
28465106 .. 28465812
707 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1206500.v1.1.491

Sequence Viewer

Length: 525 bp
ATGAGTACAAATCTTAACCCTGAAGCCGAATTCGCGTACGGTGCTGGCCTACTAAATCCTAGTAGGGCTCCGTATCCTGGTTTGGTATACGACACTGCTGAAATCGATTACGTAAATTTTTTGTGTGCACAAGGCTATAGTACCAGATTATTGAAAGCTCTTACCGGGGATAGCTATAGCTGCTCATCATCACAATCTAGTCATGGAACACTCAGTGATCATCTAAACTATCCTTCTGTTGCACTTTCCACCTCGAACCCTAAATCCGTCAATGGCATTTTCAATAGGACCGTCACAAATGTTGGATCACCAAAGTCCACATATAAAGCTAAAGTGAGTGCACCACCAGGACTTGATATCAAAGTTAATCCAAGCATTCTAAAGTTCACATCTCTCGGGCAGAAGCTATCGTTTCAAGTCACGGTGAAAGGGTTGATTGAAAAAACCATAGTCTCTGGTTCTCTGGTGTGGGATGATGGTAAATTCCAAGTGAGGAGCCCCATTGTTGTGTATTTTGTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S8

Protein Analysis

175

Amino Acids

18.73

Weight (kDa)

9.04

Isoelectric Point (pI)

29.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_6 PF17766 75 - 170 7e-30 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 87
AccII CGCG 1 cut(s) 35
AclWI GGATC 1 cut(s) 313
AcsI RAATTY 3 cut(s) 29, 115, 482
AcuI CTGAAG 1 cut(s) 42
AdeI CACNNNGTG 1 cut(s) 215
AfaI GTAC 3 cut(s) 7, 38, 142
AfiI CCNNNNNNNGG 1 cut(s) 77
AgsI TTSAA 4 cut(s) 154, 283, 416, 440
AjnI CCWGG 2 cut(s) 76, 346
AloI GAACNNNNNNTCC 2 cut(s) 248, 280
AluBI AGCT 5 cut(s) 158, 174, 180, 329, 406
AluI AGCT 5 cut(s) 158, 174, 180, 329, 406
Alw21I GWGCWC 2 cut(s) 130, 343
Alw26I GTCTC 1 cut(s) 457
Alw44I GTGCAC 2 cut(s) 126, 339
AlwI GGATC 1 cut(s) 313
Ama87I CYCGRG 1 cut(s) 395
AoxI GGCC 1 cut(s) 46
ApaLI GTGCAC 2 cut(s) 126, 339
ApeKI GCWGC 1 cut(s) 180
ApoI RAATTY 3 cut(s) 29, 115, 482
AspS9I GGNCC 1 cut(s) 288
AsuC2I CCSGG 1 cut(s) 166
AsuHPI GGTGA 2 cut(s) 300, 436
AvaI CYCGRG 1 cut(s) 395
AvaII GGWCC 1 cut(s) 288
BaeGI GKGCMC 2 cut(s) 130, 343
BanII GRGCYC 2 cut(s) 70, 500
Bbv12I GWGCWC 2 cut(s) 130, 343
BbvI GCAGC 1 cut(s) 167
BccI CCATC 1 cut(s) 470
BciT130I CCWGG 2 cut(s) 78, 348
BciVI GTATCC 1 cut(s) 84
BclI TGATCA 1 cut(s) 217
BcnI CCSGG 1 cut(s) 166
BcoDI GTCTC 1 cut(s) 457
BfaI CTAG 2 cut(s) 60, 198
BfmI CTRYAG 2 cut(s) 136, 175
BfuI GTATCC 1 cut(s) 84
BisI GCNGC 1 cut(s) 181
BlsI GCNGC 1 cut(s) 182
Bme1390I CCNGG 3 cut(s) 78, 166, 348
Bme18I GGWCC 1 cut(s) 288
BmeT110I CYCGRG 1 cut(s) 395
BmgT120I GGNCC 1 cut(s) 288
BmiI GGNNCC 2 cut(s) 69, 497
BmrFI CCNGG 3 cut(s) 78, 166, 348
BpuMI CCSGG 1 cut(s) 166
Bsa29I ATCGAT 1 cut(s) 105
BsaAI YACGTR 1 cut(s) 112
BsaJI CCNNGG 1 cut(s) 165
Bsc4I CCNNNNNNNGG 1 cut(s) 77
BseBI CCWGG 2 cut(s) 78, 348
BseCI ATCGAT 1 cut(s) 105
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 1 cut(s) 478
BseLI CCNNNNNNNGG 1 cut(s) 77
BseMII CTCAG 1 cut(s) 226
BseRI GAGGAG 1 cut(s) 508
BseSI GKGCMC 2 cut(s) 130, 343
BseXI GCAGC 1 cut(s) 167
Bsh1236I CGCG 1 cut(s) 35
BshFI GGCC 1 cut(s) 48
BshVI ATCGAT 1 cut(s) 105
BsiHKAI GWGCWC 2 cut(s) 130, 343
BsiHKCI CYCGRG 1 cut(s) 395
BsiSI CCGG 1 cut(s) 165
BsiWI CGTACG 1 cut(s) 36
BslI CCNNNNNNNGG 1 cut(s) 77
BsmAI GTCTC 1 cut(s) 457
BsmI GAATGC 1 cut(s) 375
BsnI GGCC 1 cut(s) 48
BsoBI CYCGRG 1 cut(s) 395
Bsp1286I GDGCHC 4 cut(s) 70, 130, 343, 500
Bsp143I GATC 2 cut(s) 217, 305
BspANI GGCC 1 cut(s) 48
BspCNI CTCAG 1 cut(s) 225
BspDI ATCGAT 1 cut(s) 105
BspFNI CGCG 1 cut(s) 35
BspLI GGNNCC 2 cut(s) 69, 497
BspPI GGATC 1 cut(s) 313
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 2 cut(s) 217, 305
BssNAI GTATAC 1 cut(s) 88
Bst1107I GTATAC 1 cut(s) 88
Bst2UI CCWGG 2 cut(s) 78, 348
Bst4CI ACNGT 3 cut(s) 41, 292, 424
BstBAI YACGTR 1 cut(s) 112
BstC8I GCNNGC 1 cut(s) 46
BstDEI CTNAG 1 cut(s) 212
BstF5I GGATG 1 cut(s) 478
BstFNI CGCG 1 cut(s) 35
BstKTI GATC 2 cut(s) 220, 308
BstMAI GTCTC 1 cut(s) 457
BstMBI GATC 2 cut(s) 217, 305
BstMWI GCNNNNNNNGC 3 cut(s) 32, 41, 180
BstNI CCWGG 2 cut(s) 78, 348
BstSCI CCNGG 3 cut(s) 76, 164, 346
BstSFI CTRYAG 2 cut(s) 136, 175
BstSLI GKGCMC 2 cut(s) 130, 343
BstSNI TACGTA 1 cut(s) 112
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 1 cut(s) 167
BstZ17I GTATAC 1 cut(s) 88
Bsu15I ATCGAT 1 cut(s) 105
BsuI GTATCC 1 cut(s) 84
BsuRI GGCC 1 cut(s) 48
BsuTUI ATCGAT 1 cut(s) 105
BtsCI GGATG 1 cut(s) 478
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 2 cut(s) 93, 220
Cac8I GCNNGC 1 cut(s) 46
Cfr13I GGNCC 1 cut(s) 288
ClaI ATCGAT 1 cut(s) 105
Csp6I GTAC 3 cut(s) 6, 37, 141
CviAII CATG 1 cut(s) 203
CviQI GTAC 3 cut(s) 6, 37, 141
DdeI CTNAG 1 cut(s) 212
DpnI GATC 2 cut(s) 219, 307
DpnII GATC 2 cut(s) 217, 305
DraIII CACNNNGTG 1 cut(s) 215
Eco105I TACGTA 1 cut(s) 112
Eco24I GRGCYC 2 cut(s) 70, 500
Eco32I GATATC 1 cut(s) 358
Eco47I GGWCC 1 cut(s) 288
Eco57I CTGAAG 1 cut(s) 42
Eco88I CYCGRG 1 cut(s) 395
EcoRI GAATTC 1 cut(s) 29
EcoRII CCWGG 2 cut(s) 76, 346
EcoRV GATATC 1 cut(s) 358
EcoT38I GRGCYC 2 cut(s) 70, 500
FaeI CATG 1 cut(s) 206
FaiI YATR 7 cut(s) 88, 138, 177, 204, 322, 324, 449
FatI CATG 1 cut(s) 202
FbaI TGATCA 1 cut(s) 217
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 1 cut(s) 181
FokI GGATG 1 cut(s) 485
FriOI GRGCYC 2 cut(s) 70, 500
Fsp4HI GCNGC 1 cut(s) 181
FspBI CTAG 2 cut(s) 60, 198
GluI GCNGC 1 cut(s) 181
HaeIII GGCC 1 cut(s) 48
HapII CCGG 1 cut(s) 165
Hin1II CATG 1 cut(s) 206
HpaII CCGG 1 cut(s) 165
HphI GGTGA 2 cut(s) 300, 436
Hpy166II GTNNAC 5 cut(s) 88, 128, 318, 341, 387
Hpy8I GTNNAC 5 cut(s) 88, 128, 318, 341, 387
HpyAV CCTTC 1 cut(s) 243
HpyCH4III ACNGT 3 cut(s) 41, 292, 424
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 3 cut(s) 128, 242, 341
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 41, 180
HpyF3I CTNAG 1 cut(s) 212
HpySE526I ACGT 1 cut(s) 111
Hsp92II CATG 1 cut(s) 206
Ksp22I TGATCA 1 cut(s) 217
Kzo9I GATC 2 cut(s) 217, 305
LmnI GCTCC 2 cut(s) 73, 495
Lsp1109I GCAGC 1 cut(s) 167
MaeI CTAG 2 cut(s) 60, 198
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 2 cut(s) 292, 418
MalI GATC 2 cut(s) 219, 307
MboI GATC 2 cut(s) 217, 305
MhlI GDGCHC 4 cut(s) 70, 130, 343, 500
MluCI AATT 3 cut(s) 29, 115, 482
MmeI TCCRAC 1 cut(s) 283
MnlI CCTC 2 cut(s) 262, 486
MseI TTAA 2 cut(s) 15, 366
MslI CAYNNNNRTG 1 cut(s) 506
MspI CCGG 1 cut(s) 165
MspR9I CCNGG 3 cut(s) 78, 166, 348
Mva1269I GAATGC 1 cut(s) 375
MvaI CCWGG 2 cut(s) 78, 348
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 3 cut(s) 32, 41, 180
NciI CCSGG 1 cut(s) 166
NdeII GATC 2 cut(s) 217, 305
NlaIII CATG 1 cut(s) 206
NlaIV GGNNCC 2 cut(s) 69, 497
NmuCI GTSAC 2 cut(s) 292, 418
PctI GAATGC 1 cut(s) 375
Pfl23II CGTACG 1 cut(s) 36
PkrI GCNGC 1 cut(s) 182
Ppu21I YACGTR 1 cut(s) 112
Psp6I CCWGG 2 cut(s) 76, 346
PspGI CCWGG 2 cut(s) 76, 346
PspLI CGTACG 1 cut(s) 36
PspN4I GGNNCC 2 cut(s) 69, 497
PspPI GGNCC 1 cut(s) 288
RsaI GTAC 3 cut(s) 7, 38, 142
RsaNI GTAC 3 cut(s) 6, 37, 141
RseI CAYNNNNRTG 1 cut(s) 506
SaqAI TTAA 2 cut(s) 15, 366
SatI GCNGC 1 cut(s) 181
Sau3AI GATC 2 cut(s) 217, 305
Sau96I GGNCC 1 cut(s) 288
ScrFI CCNGG 3 cut(s) 78, 166, 348
SduI GDGCHC 4 cut(s) 70, 130, 343, 500
SetI ASST 7 cut(s) 114, 160, 176, 182, 254, 331, 408
SfcI CTRYAG 2 cut(s) 136, 175
SinI GGWCC 1 cut(s) 288
SmiMI CAYNNNNRTG 1 cut(s) 506
SnaBI TACGTA 1 cut(s) 112
Sse9I AATT 3 cut(s) 29, 115, 482
SspMI CTAG 2 cut(s) 60, 198
StyD4I CCNGG 3 cut(s) 76, 164, 346
TaaI ACNGT 3 cut(s) 41, 292, 424
TaiI ACGT 1 cut(s) 114
TaqI TCGA 2 cut(s) 105, 254
TasI AATT 3 cut(s) 29, 115, 482
TatI WGTACW 1 cut(s) 5
Tru1I TTAA 2 cut(s) 15, 366
Tru9I TTAA 2 cut(s) 15, 366
TscAI CASTG 2 cut(s) 100, 220
TseFI GTSAC 2 cut(s) 292, 418
TseI GCWGC 1 cut(s) 180
Tsp45I GTSAC 2 cut(s) 292, 418
TspGWI ACGGA 2 cut(s) 60, 256
TspRI CASTG 2 cut(s) 100, 220
VneI GTGCAC 2 cut(s) 126, 339
VpaK11BI GGWCC 1 cut(s) 288
XapI RAATTY 3 cut(s) 29, 115, 482
XmiI GTMKAC 1 cut(s) 87
XspI CTAG 2 cut(s) 60, 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.