pycom07g19280

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000390
Physical Location & Seq
Forward (+)
21642733 .. 21644331
1599 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g19280.2

Sequence Viewer

Length: 1020 bp
ATGGCTTTAACTCCATGGTTTCTCCTTCTCATCAGCCTCATATCTACTCTGCTAGTTGATGTCACTCTGTCTGCTGCTCACCAGGATAACCGGAAGGATTATATAGTGTATATGGGTGACAAGCCAAAGCCTGAGGTTTCCACCACTACCACATCAGCTCTTCATGTAAACATGCTACAAAACGTCGTCGACGACAGCAATATTGCGCACGAATCTCTGCTTCTGCACAGCTACAAGAGAAGCTTCAATGGCTTTGCTGCCAGGCTAACAGAGGAAGAAGCACAGAAGTTGGCTGGAATGGATGGTGTGGTGTCTGTTTTCCCTAGTGAAACTAAGAAGCTCCAAACAACAAGGTCATGGGACTTCATTGGGTTTCCTGAAATGGTGAAGAGAAGCGCCATTGAAACGGATATCATTATCGGGGTGATTGACTATGGAATTTGGCCTGAATCCGCCAGCTTCAGTGACGCCGGGTTTGGTCCACCCCCCAAAAGGTGGAAAGGCGCCTGCAAGGGCGAAGGCAATTTTACTTGCAACAATAAAATTATCGGAGCACGGTATTACCGCAGTCTACCCTACCCCAAAAATAGCAGTGATATCCTGTCCCCGAGAGACACGGAAGGCCATGGAACCCACTGTGCATCAACAGCAGCAGGGAACTTAGTTAGCAAGGCAAGTCTGTATGGTTTAGGGTTGGGGACAGCAAGAGGAGGGGTGCCATCAGCACGCATTGCGGTGTACAAAGTTTGTTGGTCAGAAGGGTGCCCGGATGCTGATATACTAGCCGCATTCGATGATGCCATAGCTGACGGTGTTGACATACTCTCTGTCTCCCTTGGGGGGCTTAAACCGTTAGATTATTTCAGAAATTCCATTGACATTGGAGCTTTTCACGCTTTAAGAAAAGGAATATTCACTTCCGCATCTGCTGGTAATGAAGGTCCAAACCTGAAAACTATTACAAACTTTGCACCATGGTCTCTTGCTGTGGCTGCTAGCACCATAGATCGATCACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

340

Amino Acids

36.49

Weight (kDa)

6.17

Isoelectric Point (pI)

35.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 207
AccB1I GGYRCC 3 cut(s) 503, 715, 762
AccB7I CCANNNNNTGG 1 cut(s) 495
AccI GTMKAC 2 cut(s) 189, 571
AciI CCGC 5 cut(s) 453, 565, 734, 786, 921
AcsI RAATTY 2 cut(s) 438, 868
AcuI CTGAAG 1 cut(s) 445
AcyI GRCGYC 2 cut(s) 468, 504
AfaI GTAC 1 cut(s) 740
AfiI CCNNNNNNNGG 3 cut(s) 492, 495, 840
AgsI TTSAA 2 cut(s) 247, 404
AjnI CCWGG 2 cut(s) 81, 260
AluBI AGCT 7 cut(s) 158, 231, 243, 340, 459, 806, 887
AluI AGCT 7 cut(s) 158, 231, 243, 340, 459, 806, 887
Alw21I GWGCWC 1 cut(s) 556
Alw26I GTCTC 3 cut(s) 606, 835, 984
Ama87I CYCGRG 1 cut(s) 607
AoxI GGCC 2 cut(s) 443, 622
ApeKI GCWGC 4 cut(s) 74, 257, 650, 992
ApoI RAATTY 2 cut(s) 438, 868
ArsI GACNNNNNNTTYG 2 cut(s) 458, 490
AspLEI GCGC 3 cut(s) 208, 398, 506
AspS9I GGNCC 2 cut(s) 479, 941
AsuC2I CCSGG 2 cut(s) 472, 767
AsuHPI GGTGA 4 cut(s) 71, 128, 397, 436
AsuNHI GCTAGC 1 cut(s) 995
AvaI CYCGRG 1 cut(s) 607
AvaII GGWCC 2 cut(s) 479, 941
AxyI CCTNAGG 1 cut(s) 132
BaeGI GKGCMC 1 cut(s) 767
BanI GGYRCC 3 cut(s) 503, 715, 762
Bbv12I GWGCWC 1 cut(s) 556
BbvI GCAGC 4 cut(s) 61, 244, 662, 979
BccI CCATC 2 cut(s) 296, 727
BciT130I CCWGG 2 cut(s) 83, 262
BcnI CCSGG 2 cut(s) 472, 767
BcoDI GTCTC 3 cut(s) 606, 835, 984
BfaI CTAG 4 cut(s) 53, 324, 782, 996
BfoI RGCGCY 2 cut(s) 399, 507
BisI GCNGC 5 cut(s) 75, 258, 651, 786, 993
BlsI GCNGC 5 cut(s) 76, 259, 652, 787, 994
Bme1390I CCNGG 4 cut(s) 83, 262, 472, 767
Bme18I GGWCC 2 cut(s) 479, 941
BmeT110I CYCGRG 1 cut(s) 607
BmgT120I GGNCC 2 cut(s) 479, 941
BmiI GGNNCC 4 cut(s) 505, 631, 717, 764
BmrFI CCNGG 4 cut(s) 83, 262, 472, 767
BmsI GCATC 4 cut(s) 650, 760, 787, 932
BmtI GCTAGC 1 cut(s) 999
BpuMI CCSGG 2 cut(s) 472, 767
Bsa29I ATCGAT 1 cut(s) 1009
BsaHI GRCGYC 2 cut(s) 468, 504
BsaI GGTCTC 1 cut(s) 984
BsaJI CCNNGG 4 cut(s) 14, 625, 835, 974
BsaWI WCCGGW 1 cut(s) 90
Bsc4I CCNNNNNNNGG 3 cut(s) 492, 495, 840
Bse21I CCTNAGG 1 cut(s) 132
Bse3DI GCAATG 1 cut(s) 729
BseBI CCWGG 2 cut(s) 83, 262
BseCI ATCGAT 1 cut(s) 1009
BseDI CCNNGG 4 cut(s) 14, 625, 835, 974
BseGI GGATG 2 cut(s) 307, 775
BseLI CCNNNNNNNGG 3 cut(s) 492, 495, 840
BseMI GCAATG 1 cut(s) 729
BseMII CTCAG 1 cut(s) 123
BseRI GAGGAG 1 cut(s) 723
BseSI GKGCMC 1 cut(s) 767
BseXI GCAGC 4 cut(s) 61, 244, 662, 979
BsgI GTGCAG 1 cut(s) 209
BshFI GGCC 2 cut(s) 445, 624
BshNI GGYRCC 3 cut(s) 503, 715, 762
BshVI ATCGAT 1 cut(s) 1009
BsiHKAI GWGCWC 1 cut(s) 556
BsiHKCI CYCGRG 1 cut(s) 607
BsiSI CCGG 3 cut(s) 91, 471, 767
BslFI GGGAC 3 cut(s) 374, 589, 712
BslI CCNNNNNNNGG 3 cut(s) 492, 495, 840
BsmAI GTCTC 3 cut(s) 606, 835, 984
BsmFI GGGAC 3 cut(s) 374, 589, 712
BsmI GAATGC 1 cut(s) 788
BsnI GGCC 2 cut(s) 445, 624
Bso31I GGTCTC 1 cut(s) 984
BsoBI CYCGRG 1 cut(s) 607
Bsp1286I GDGCHC 2 cut(s) 556, 767
Bsp1407I TGTACA 1 cut(s) 738
Bsp143I GATC 2 cut(s) 1006, 1010
Bsp19I CCATGG 3 cut(s) 14, 625, 974
BspACI CCGC 5 cut(s) 453, 565, 734, 786, 921
BspANI GGCC 2 cut(s) 445, 624
BspCNI CTCAG 1 cut(s) 124
BspDI ATCGAT 1 cut(s) 1009
BspLI GGNNCC 4 cut(s) 505, 631, 717, 764
BspOI GCTAGC 1 cut(s) 999
BspQI GCTCTTC 1 cut(s) 165
BspT107I GGYRCC 3 cut(s) 503, 715, 762
BspTNI GGTCTC 1 cut(s) 984
BsrDI GCAATG 1 cut(s) 729
BsrGI TGTACA 1 cut(s) 738
BssECI CCNNGG 4 cut(s) 14, 625, 835, 974
BssMI GATC 2 cut(s) 1006, 1010
BssNI GRCGYC 2 cut(s) 468, 504
BssT1I CCWWGG 4 cut(s) 14, 625, 835, 974
Bst2UI CCWGG 2 cut(s) 83, 262
Bst4CI ACNGT 4 cut(s) 558, 638, 812, 852
Bst6I CTCTTC 2 cut(s) 165, 383
BstACI GRCGYC 2 cut(s) 468, 504
BstAPI GCANNNNNTGC 1 cut(s) 731
BstAUI TGTACA 1 cut(s) 738
BstC8I GCNNGC 4 cut(s) 457, 508, 727, 997
BstDEI CTNAG 3 cut(s) 132, 333, 661
BstDSI CCRYGG 3 cut(s) 14, 625, 974
BstF5I GGATG 2 cut(s) 307, 775
BstH2I RGCGCY 2 cut(s) 399, 507
BstHHI GCGC 3 cut(s) 208, 398, 506
BstKTI GATC 2 cut(s) 1009, 1013
BstMAI GTCTC 3 cut(s) 606, 835, 984
BstMBI GATC 2 cut(s) 1006, 1010
BstMWI GCNNNNNNNGC 5 cut(s) 249, 647, 731, 893, 992
BstNI CCWGG 2 cut(s) 83, 262
BstNSI RCATGY 1 cut(s) 175
BstSCI CCNGG 4 cut(s) 81, 260, 470, 765
BstSLI GKGCMC 1 cut(s) 767
BstV1I GCAGC 4 cut(s) 61, 244, 662, 979
Bsu15I ATCGAT 1 cut(s) 1009
Bsu36I CCTNAGG 1 cut(s) 132
BsuRI GGCC 2 cut(s) 445, 624
BsuTUI ATCGAT 1 cut(s) 1009
BtgI CCRYGG 3 cut(s) 14, 625, 974
BtsCI GGATG 2 cut(s) 307, 775
BtsI GCAGTG 1 cut(s) 598
BtsIMutI CAGTG 3 cut(s) 469, 598, 634
Cac8I GCNNGC 4 cut(s) 457, 508, 727, 997
CfoI GCGC 3 cut(s) 208, 398, 506
Cfr13I GGNCC 2 cut(s) 479, 941
ClaI ATCGAT 1 cut(s) 1009
CseI GACGC 1 cut(s) 476
Csp6I GTAC 1 cut(s) 739
CviAII CATG 6 cut(s) 15, 164, 172, 357, 626, 975
CviQI GTAC 1 cut(s) 739
DdeI CTNAG 3 cut(s) 132, 333, 661
DinI GGCGCC 1 cut(s) 505
DpnI GATC 2 cut(s) 1008, 1012
DpnII GATC 2 cut(s) 1006, 1010
Eam1104I CTCTTC 2 cut(s) 165, 383
EarI CTCTTC 2 cut(s) 165, 383
EciI GGCGGA 1 cut(s) 442
Eco130I CCWWGG 4 cut(s) 14, 625, 835, 974
Eco31I GGTCTC 1 cut(s) 984
Eco32I GATATC 2 cut(s) 412, 598
Eco47I GGWCC 2 cut(s) 479, 941
Eco57I CTGAAG 1 cut(s) 445
Eco81I CCTNAGG 1 cut(s) 132
Eco88I CYCGRG 1 cut(s) 607
EcoRII CCWGG 2 cut(s) 81, 260
EcoRV GATATC 2 cut(s) 412, 598
EcoT14I CCWWGG 4 cut(s) 14, 625, 835, 974
EgeI GGCGCC 1 cut(s) 505
EheI GGCGCC 1 cut(s) 505
ErhI CCWWGG 4 cut(s) 14, 625, 835, 974
FaeI CATG 6 cut(s) 18, 167, 175, 360, 629, 978
FalI AAGNNNNNCTT 2 cut(s) 227, 259
FaqI GGGAC 3 cut(s) 374, 589, 712
FatI CATG 6 cut(s) 14, 163, 171, 356, 625, 974
FblI GTMKAC 2 cut(s) 189, 571
Fnu4HI GCNGC 5 cut(s) 75, 258, 651, 786, 993
FokI GGATG 2 cut(s) 314, 782
Fsp4HI GCNGC 5 cut(s) 75, 258, 651, 786, 993
FspBI CTAG 4 cut(s) 53, 324, 782, 996
FspI TGCGCA 1 cut(s) 207
GlaI GCGC 3 cut(s) 207, 397, 505
GluI GCNGC 5 cut(s) 75, 258, 651, 786, 993
HaeII RGCGCY 2 cut(s) 399, 507
HaeIII GGCC 2 cut(s) 445, 624
HapII CCGG 3 cut(s) 91, 471, 767
HgaI GACGC 1 cut(s) 476
HhaI GCGC 3 cut(s) 208, 398, 506
Hin1I GRCGYC 2 cut(s) 468, 504
Hin1II CATG 6 cut(s) 18, 167, 175, 360, 629, 978
Hin6I GCGC 3 cut(s) 206, 396, 504
HinP1I GCGC 3 cut(s) 206, 396, 504
HincII GTYRAC 2 cut(s) 190, 817
HindII GTYRAC 2 cut(s) 190, 817
HindIII AAGCTT 1 cut(s) 241
HinfI GANTC 2 cut(s) 212, 449
HpaII CCGG 3 cut(s) 91, 471, 767
HphI GGTGA 4 cut(s) 71, 128, 397, 436
Hpy166II GTNNAC 6 cut(s) 169, 190, 482, 572, 739, 817
Hpy188I TCNGA 3 cut(s) 551, 757, 866
Hpy188III TCNNGA 1 cut(s) 377
Hpy8I GTNNAC 6 cut(s) 169, 190, 482, 572, 739, 817
Hpy99I CGWCG 3 cut(s) 188, 191, 194
HpyAV CCTTC 6 cut(s) 35, 88, 512, 614, 752, 932
HpyCH4III ACNGT 4 cut(s) 558, 638, 812, 852
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 5 cut(s) 226, 510, 534, 641, 971
HpyF10VI GCNNNNNNNGC 5 cut(s) 249, 647, 731, 893, 992
HpyF3I CTNAG 3 cut(s) 132, 333, 661
HpySE526I ACGT 1 cut(s) 183
Hsp92I GRCGYC 2 cut(s) 468, 504
Hsp92II CATG 6 cut(s) 18, 167, 175, 360, 629, 978
HspAI GCGC 3 cut(s) 206, 396, 504
KasI GGCGCC 1 cut(s) 503
Kzo9I GATC 2 cut(s) 1006, 1010
LguI GCTCTTC 1 cut(s) 165
LmnI GCTCC 3 cut(s) 345, 551, 884
Lsp1109I GCAGC 4 cut(s) 61, 244, 662, 979
LweI GCATC 4 cut(s) 650, 760, 787, 932
MaeI CTAG 4 cut(s) 53, 324, 782, 996
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 3 cut(s) 61, 116, 464
MalI GATC 2 cut(s) 1008, 1012
MboI GATC 2 cut(s) 1006, 1010
MboII GAAGA 3 cut(s) 152, 287, 400
MhlI GDGCHC 2 cut(s) 556, 767
MluCI AATT 4 cut(s) 438, 523, 543, 868
Mly113I GGCGCC 1 cut(s) 504
MnlI CCTC 5 cut(s) 47, 127, 265, 701, 704
MseI TTAA 3 cut(s) 8, 846, 899
MslI CAYNNNNRTG 1 cut(s) 734
MspI CCGG 3 cut(s) 91, 471, 767
MspR9I CCNGG 4 cut(s) 83, 262, 472, 767
Mva1269I GAATGC 1 cut(s) 788
MvaI CCWGG 2 cut(s) 83, 262
MwoI GCNNNNNNNGC 5 cut(s) 249, 647, 731, 893, 992
NarI GGCGCC 1 cut(s) 504
NciI CCSGG 2 cut(s) 472, 767
NcoI CCATGG 3 cut(s) 14, 625, 974
NdeII GATC 2 cut(s) 1006, 1010
NheI GCTAGC 1 cut(s) 995
NlaIII CATG 6 cut(s) 18, 167, 175, 360, 629, 978
NlaIV GGNNCC 4 cut(s) 505, 631, 717, 764
NmuCI GTSAC 3 cut(s) 61, 116, 464
NsbI TGCGCA 1 cut(s) 207
NspI RCATGY 1 cut(s) 175
PciSI GCTCTTC 1 cut(s) 165
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PctI GAATGC 1 cut(s) 788
PfeI GAWTC 2 cut(s) 212, 449
PflMI CCANNNNNTGG 1 cut(s) 495
PkrI GCNGC 5 cut(s) 76, 259, 652, 787, 994
PluTI GGCGCC 1 cut(s) 507
Psp6I CCWGG 2 cut(s) 81, 260
PspGI CCWGG 2 cut(s) 81, 260
PspN4I GGNNCC 4 cut(s) 505, 631, 717, 764
PspPI GGNCC 2 cut(s) 479, 941
RsaI GTAC 1 cut(s) 740
RsaNI GTAC 1 cut(s) 739
RseI CAYNNNNRTG 1 cut(s) 734
SalI GTCGAC 1 cut(s) 188
SapI GCTCTTC 1 cut(s) 165
SaqAI TTAA 3 cut(s) 8, 846, 899
SatI GCNGC 5 cut(s) 75, 258, 651, 786, 993
Sau3AI GATC 2 cut(s) 1006, 1010
Sau96I GGNCC 2 cut(s) 479, 941
ScrFI CCNGG 4 cut(s) 83, 262, 472, 767
SduI GDGCHC 2 cut(s) 556, 767
SfaNI GCATC 4 cut(s) 650, 760, 787, 932
SfoI GGCGCC 1 cut(s) 505
SgrDI CGTCGACG 1 cut(s) 188
SinI GGWCC 2 cut(s) 479, 941
SmiMI CAYNNNNRTG 1 cut(s) 734
Sse9I AATT 4 cut(s) 438, 523, 543, 868
SsiI CCGC 5 cut(s) 453, 565, 734, 786, 921
SspDI GGCGCC 1 cut(s) 503
SspI AATATT 2 cut(s) 202, 912
SspMI CTAG 4 cut(s) 53, 324, 782, 996
StyD4I CCNGG 4 cut(s) 81, 260, 470, 765
StyI CCWWGG 4 cut(s) 14, 625, 835, 974
TaaI ACNGT 4 cut(s) 558, 638, 812, 852
TaiI ACGT 1 cut(s) 186
TaqI TCGA 3 cut(s) 189, 792, 1009
TasI AATT 4 cut(s) 438, 523, 543, 868
TatI WGTACW 1 cut(s) 738
TauI GCSGC 1 cut(s) 788
TfiI GAWTC 2 cut(s) 212, 449
Tru1I TTAA 3 cut(s) 8, 846, 899
Tru9I TTAA 3 cut(s) 8, 846, 899
TscAI CASTG 3 cut(s) 469, 598, 641
TseFI GTSAC 3 cut(s) 61, 116, 464
TseI GCWGC 4 cut(s) 74, 257, 650, 992
Tsp45I GTSAC 3 cut(s) 61, 116, 464
TspDTI ATGAA 3 cut(s) 152, 355, 951
TspGWI ACGGA 2 cut(s) 422, 632
TspRI CASTG 3 cut(s) 469, 598, 641
Van91I CCANNNNNTGG 1 cut(s) 495
VpaK11BI GGWCC 2 cut(s) 479, 941
XapI RAATTY 2 cut(s) 438, 868
XceI RCATGY 1 cut(s) 175
XmiI GTMKAC 2 cut(s) 189, 571
XspI CTAG 4 cut(s) 53, 324, 782, 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.