Prupe.2G242000_v2.0.a1

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000390
Physical Location & Seq
Forward (+)
26101403 .. 26105202
3800 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G242000.1

Sequence Viewer

Length: 2259 bp
ATGGATTTAGTTCCATGGTCGCGAAAAGAGTTTTTCCTTGTAGTGTGGTTTCTCCTTCTCAGCTTCACATGTACTCTGCTAGTAGATGTCACTCATTCTGCTGCTCATCCGGATACCAGAAAGGTTTATATTGTGTACATGGGGGACAAGCCAAAGATCGACATTCCCACCACTACCACATTGCCTCTTCATCTAAACATGCTTCAAAACGTAGTTGGCAGCAGCAATATTGAACAAGAACCTCTCCTACTGCACAGCTACAAGAGAAGCTTCAATGGCTTTGCTGCAAAGCTAACAGAGGAAGAAGCTCAGAAAATGGCTGGAATGGCTGGTGTGGTGTCTGTTTTCCCTAGTAGGAAGCAGAAGCTCCACACAACAAGGTCATGGAACTTCATCGGGTTTCATGAAAACGTGAAGAGAAGCACTGTTGAAAGCGATATCATCGTTGGGATGATCGACTCTGGAGTTTGGCCTGAATCTGCCAGCTTCAGTGATGCCGGGTTCGGCCCACCACCCAAAAAATGGAAAGGCACATGCCAAGGCTTATCCAATTTTACTTGCAACAACAAAATCATTGGAGCACGGTATTACCATAATGGTAGACCTTTTATCAAAGGTGACATCAAGTCTCCACGAGACTCGGATGGTCATGGAACTCACACTGCATCAACGGCAGCGGGAAACTTAGTTAGCAAAGCAAGCCTATTTGTTTTGGGGTCGGGGACAGCAAGAGGAGGGGTCCCATCAGCACGTATTGCGGTGTACAAAGTAGGTTGGTCGGATGGTATTAGTGACGATGATATTCTAGCGGCATTTGATGATGCGATCGCGGATGGTGTCGACATACTATCTCTCTCCCTCGGAAAGGCTGAGGACGATTATTTCAGGGACTCAATTTCCATTGGAGCCTTTCACGCTTTAAGAAAAGGAATTCTGACTTCCACTGCTGCTGGGAACGACGGTCCAGGCCCCAAGACCATCGCAAACTTTGCGCCATGGTTTCTTTCTGTGGCTGCAACCACCATAGATCGGGAGTTTGTTACCAAGGTTCAATTGGGTAACCAAAAAATCTATGAGGGAATTGTAACAAACACATTTGACCTCAAGGGCAAGTTCTATCCTCTAATACATGCTGGAGACGCACCCAATAGGAAAGCAGGCTACGACGGGTCTACATCTATGAAATGCAAACCAGGCACGTTAGACCACAATTTGGTGAAGGGCAAGATTGTTCTTTGCGATGGGAAGAATGGGTATGGGGCCTATTTTGCAGGTGCAGTTGGAGTTATTTTGCAAAACCGACCTGTTGCAGATGTGCTTGACCCTCTTCCCATGCCTGCCTCTTGCCTTGGTTTGGATAGTGGTAACAGCATTTACCATTACATTAACTCAACAAGGAACCCAACTGCAACAATTTTTAAAAGTACTGAGGATATTGACACACTGTCCCCATATGTGCCATCCTTCTCATCAAGGGGTCCTAATCCAGTCACTCCTAACATTCTCAAGCCAGATATAGCTGCTCCGGGAGCTTCTATTTTGGCAGCATGGCCTCCAATTGCCCCGGTTTCGGCCTATCCAGGAGATGACAGAGTAGCATCATACAATGTAATCTCAGGGACATCAATGGCTTGCCCACATGCCACTGGGGTAGCTGCATACGTCAAATCATTTCACCCCAATTGGACTCCTGCTGCTATCCAATCCGCTCTTATTACCACCGCTAAACCCCTGAGTCCCGATCTTAACCCTGAAGCCGAATTTGCATATGGAGCTGGCCAAATTGACCCTGTTAGGGCTCCATATCCTGGTTTGGTATATGATGCTACGGAGCTGGACTACATAGAATTTTTGTGTGCACAAGGCTACAGTACCAAATTATTGCAATCTATTACCGGGCACAAGAGTTGCTGCTCATCAAAAACTAATTATGGAGCACTCAGTGATAATTTGAATTATCCTTCTTTTGCACTTTCCTCCTCTAATCCAAACTCCATCAGTGGGGTTTTCAATAGGACAGCCACAAATGTTGGATCACCAAGGTCCACATATAAAGCTAAAGTGATTGGTGCAACGAAAGGACTCGAAATCAAAGTTAATCCAAGCATTCTATCGTTCTCATCTCTCGGGCAGAAGCTATCCTTTCAGGTCACGATAAAAGGGTCAATTCATCGCAAATCCAGTGTCTCTGCTTCTCTGGTGTTTGATGATGGTACTTTCCAAGTCAGGAGCCCCATTGTTGTCTATGCTATATATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

753

Amino Acids

80.59

Weight (kDa)

8.64

Isoelectric Point (pI)

34.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1262
Acc36I ACCTGC 1 cut(s) 1262
AccB7I CCANNNNNTGG 3 cut(s) 522, 1213, 1808
AccBSI CCGCTC 1 cut(s) 1709
AccI GTMKAC 3 cut(s) 601, 840, 1172
AccII CGCG 2 cut(s) 22, 830
AccIII TCCGGA 1 cut(s) 109
AciI CCGC 6 cut(s) 677, 758, 809, 830, 1707, 1722
AclWI GGATC 1 cut(s) 2041
AcoI YGGCCR 1 cut(s) 1777
AcsI RAATTY 3 cut(s) 930, 1760, 1847
AcuI CTGAAG 2 cut(s) 472, 1773
AdeI CACNNNGTG 1 cut(s) 1943
AfaI GTAC 6 cut(s) 73, 137, 764, 1426, 1873, 2215
AfiI CCNNNNNNNGG 9 cut(s) 522, 865, 1029, 1213, 1354, 1570, 1795, 1808, 2001
AflIII ACRYGT 1 cut(s) 68
AgsI TTSAA 7 cut(s) 206, 233, 274, 431, 1052, 1954, 2011
AhdI GACNNNNNGTC 2 cut(s) 625, 1444
AjnI CCWGG 4 cut(s) 964, 1192, 1579, 1807
Alw21I GWGCWC 3 cut(s) 583, 1861, 1939
Alw26I GTCTC 4 cut(s) 630, 633, 1131, 2191
Alw44I GTGCAC 1 cut(s) 1857
AlwI GGATC 1 cut(s) 2041
Ama87I CYCGRG 1 cut(s) 2126
Aor13HI TCCGGA 1 cut(s) 109
AoxI GGCC 7 cut(s) 470, 505, 967, 1260, 1550, 1572, 1777
ApaLI GTGCAC 1 cut(s) 1857
ApoI RAATTY 3 cut(s) 930, 1760, 1847
ArsI GACNNNNNNTTYG 2 cut(s) 2169, 2201
AsiSI GCGATCGC 1 cut(s) 828
AspLEI GCGC 1 cut(s) 994
AspS9I GGNCC 7 cut(s) 506, 739, 962, 968, 1260, 1478, 2043
AsuC2I CCSGG 4 cut(s) 499, 1527, 1565, 1897
AsuHPI GGTGA 4 cut(s) 629, 1228, 1667, 2028
AvaI CYCGRG 1 cut(s) 2126
AvaII GGWCC 4 cut(s) 739, 962, 1478, 2043
BaeGI GKGCMC 2 cut(s) 1861, 1902
BalI TGGCCA 1 cut(s) 1779
BanII GRGCYC 2 cut(s) 1801, 2234
BauI CACGAG 1 cut(s) 633
Bbv12I GWGCWC 3 cut(s) 583, 1861, 1939
BbvCI CCTCAGC 1 cut(s) 870
BccI CCATC 9 cut(s) 638, 751, 776, 827, 986, 1235, 1468, 2003, 2204
BceAI ACGGC 1 cut(s) 687
BciT130I CCWGG 4 cut(s) 966, 1194, 1581, 1809
BciVI GTATCC 1 cut(s) 106
BcnI CCSGG 4 cut(s) 499, 1527, 1565, 1897
BcoDI GTCTC 4 cut(s) 630, 633, 1131, 2191
BfaI CTAG 3 cut(s) 80, 351, 806
BfmI CTRYAG 1 cut(s) 1867
BfuAI ACCTGC 1 cut(s) 1262
BfuI GTATCC 1 cut(s) 106
BmcAI AGTACT 1 cut(s) 1426
Bme1390I CCNGG 8 cut(s) 499, 966, 1194, 1527, 1565, 1581, 1809, 1897
Bme18I GGWCC 4 cut(s) 739, 962, 1478, 2043
BmeRI GACNNNNNGTC 2 cut(s) 625, 1444
BmeT110I CYCGRG 1 cut(s) 2126
BmgT120I GGNCC 7 cut(s) 506, 739, 962, 968, 1260, 1478, 2043
BmiI GGNNCC 9 cut(s) 740, 741, 907, 970, 1261, 1400, 1479, 1800, 2231
BmrFI CCNGG 8 cut(s) 499, 966, 1194, 1527, 1565, 1581, 1809, 1897
BmrI ACTGGG 1 cut(s) 1656
BmsI GCATC 5 cut(s) 484, 674, 811, 1607, 1813
BmuI ACTGGG 1 cut(s) 1656
BpmI CTGGAG 2 cut(s) 483, 1155
Bpu10I CCTNAGC 1 cut(s) 870
BpuEI CTTGAG 2 cut(s) 1088, 1490
BpuMI CCSGG 4 cut(s) 499, 1527, 1565, 1897
BsaAI YACGTR 1 cut(s) 752
BsaJI CCNNGG 8 cut(s) 14, 538, 859, 995, 1044, 1348, 1563, 2039
BsaWI WCCGGW 1 cut(s) 109
Bsc4I CCNNNNNNNGG 9 cut(s) 522, 865, 1029, 1213, 1354, 1570, 1795, 1808, 2001
Bse1I ACTGG 3 cut(s) 1487, 1651, 2181
Bse3DI GCAATG 1 cut(s) 179
BseAI TCCGGA 1 cut(s) 109
BseBI CCWGG 4 cut(s) 966, 1194, 1581, 1809
BseDI CCNNGG 8 cut(s) 14, 538, 859, 995, 1044, 1348, 1563, 2039
BseGI GGATG 6 cut(s) 106, 456, 649, 787, 838, 1460
BseLI CCNNNNNNNGG 9 cut(s) 522, 865, 1029, 1213, 1354, 1570, 1795, 1808, 2001
BseMI GCAATG 1 cut(s) 179
BseMII CTCAG 7 cut(s) 73, 323, 861, 1419, 1629, 1724, 1954
BseNI ACTGG 3 cut(s) 1487, 1651, 2181
BseRI GAGGAG 2 cut(s) 747, 1969
BseSI GKGCMC 2 cut(s) 1861, 1902
BseYI CCCAGC 1 cut(s) 950
BsgI GTGCAG 2 cut(s) 236, 1296
Bsh1236I CGCG 2 cut(s) 22, 830
Bsh1285I CGRYCG 1 cut(s) 828
BshFI GGCC 7 cut(s) 472, 507, 969, 1262, 1552, 1574, 1779
BsiEI CGRYCG 1 cut(s) 828
BsiHKAI GWGCWC 3 cut(s) 583, 1861, 1939
BsiHKCI CYCGRG 1 cut(s) 2126
BsiSI CCGG 5 cut(s) 110, 498, 1526, 1565, 1896
BslFI GGGAC 7 cut(s) 158, 725, 736, 902, 1432, 1633, 1722
BslI CCNNNNNNNGG 9 cut(s) 522, 865, 1029, 1213, 1354, 1570, 1795, 1808, 2001
BsmAI GTCTC 4 cut(s) 630, 633, 1131, 2191
BsmBI CGTCTC 1 cut(s) 1131
BsmFI GGGAC 7 cut(s) 158, 725, 736, 902, 1432, 1633, 1722
BsmI GAATGC 1 cut(s) 2106
BsnI GGCC 7 cut(s) 472, 507, 969, 1262, 1552, 1574, 1779
BsoBI CYCGRG 1 cut(s) 2126
Bsp1286I GDGCHC 6 cut(s) 583, 1801, 1861, 1902, 1939, 2234
Bsp13I TCCGGA 1 cut(s) 109
Bsp1407I TGTACA 2 cut(s) 135, 762
Bsp143I GATC 6 cut(s) 156, 453, 825, 1027, 1741, 2033
Bsp19I CCATGG 2 cut(s) 14, 995
Bsp68I TCGCGA 1 cut(s) 22
BspACI CCGC 6 cut(s) 677, 758, 809, 830, 1707, 1722
BspANI GGCC 7 cut(s) 472, 507, 969, 1262, 1552, 1574, 1779
BspCNI CTCAG 7 cut(s) 72, 322, 862, 1420, 1628, 1725, 1953
BspEI TCCGGA 1 cut(s) 109
BspFNI CGCG 2 cut(s) 22, 830
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 9 cut(s) 740, 741, 907, 970, 1261, 1400, 1479, 1800, 2231
BspMI ACCTGC 1 cut(s) 1262
BspPI GGATC 1 cut(s) 2041
BsrBI CCGCTC 1 cut(s) 1709
BsrDI GCAATG 1 cut(s) 179
BsrGI TGTACA 2 cut(s) 135, 762
BsrI ACTGG 3 cut(s) 1487, 1651, 2181
BssECI CCNNGG 8 cut(s) 14, 538, 859, 995, 1044, 1348, 1563, 2039
BssMI GATC 6 cut(s) 156, 453, 825, 1027, 1741, 2033
BssSI CACGAG 1 cut(s) 633
BssT1I CCWWGG 6 cut(s) 14, 538, 995, 1044, 1348, 2039
Bst2BI CACGAG 1 cut(s) 633
Bst2UI CCWGG 4 cut(s) 966, 1194, 1581, 1809
Bst4CI ACNGT 5 cut(s) 427, 585, 962, 1446, 1871
Bst6I CTCTTC 3 cut(s) 192, 410, 1332
BstAPI GCANNNNNTGC 2 cut(s) 755, 989
BstAUI TGTACA 2 cut(s) 135, 762
BstBAI YACGTR 1 cut(s) 752
BstC8I GCNNGC 6 cut(s) 484, 700, 1159, 1338, 1633, 1777
BstDEI CTNAG 8 cut(s) 59, 309, 685, 870, 1428, 1615, 1733, 1940
BstDSI CCRYGG 2 cut(s) 14, 995
BstEII GGTNACC 1 cut(s) 1058
BstENI CCTNNNNNAGG 1 cut(s) 863
BstF5I GGATG 6 cut(s) 106, 456, 649, 787, 838, 1460
BstFNI CGCG 2 cut(s) 22, 830
BstHHI GCGC 1 cut(s) 994
BstKTI GATC 6 cut(s) 159, 456, 828, 1030, 1744, 2036
BstMAI GTCTC 4 cut(s) 630, 633, 1131, 2191
BstMBI GATC 6 cut(s) 156, 453, 825, 1027, 1741, 2033
BstMCI CGRYCG 1 cut(s) 828
BstNI CCWGG 4 cut(s) 966, 1194, 1581, 1809
BstNSI RCATGY 5 cut(s) 72, 202, 537, 1133, 1643
BstPI GGTNACC 1 cut(s) 1058
BstSCI CCNGG 8 cut(s) 497, 964, 1192, 1525, 1563, 1579, 1807, 1895
BstSFI CTRYAG 1 cut(s) 1867
BstSLI GKGCMC 2 cut(s) 1861, 1902
BstUI CGCG 2 cut(s) 22, 830
BsuI GTATCC 1 cut(s) 106
BsuRI GGCC 7 cut(s) 472, 507, 969, 1262, 1552, 1574, 1779
BtgI CCRYGG 2 cut(s) 14, 995
BtgZI GCGATG 3 cut(s) 964, 1254, 2156
BtsCI GGATG 6 cut(s) 106, 456, 649, 787, 838, 1460
BtsI GCAGTG 2 cut(s) 660, 942
BtsIMutI CAGTG 9 cut(s) 423, 496, 660, 942, 1442, 1644, 1948, 2005, 2188
BtuMI TCGCGA 1 cut(s) 22
BveI ACCTGC 1 cut(s) 1262
Cac8I GCNNGC 6 cut(s) 484, 700, 1159, 1338, 1633, 1777
CciI TCATGA 1 cut(s) 403
CfoI GCGC 1 cut(s) 994
Cfr13I GGNCC 7 cut(s) 506, 739, 962, 968, 1260, 1478, 2043
CseI GACGC 1 cut(s) 1148
Csp6I GTAC 6 cut(s) 72, 136, 763, 1425, 1872, 2214
CspCI CAANNNNNGTGG 2 cut(s) 2011, 2046
CviQI GTAC 6 cut(s) 72, 136, 763, 1425, 1872, 2214
DdeI CTNAG 8 cut(s) 59, 309, 685, 870, 1428, 1615, 1733, 1940
DpnI GATC 6 cut(s) 158, 455, 827, 1029, 1743, 2035
DpnII GATC 6 cut(s) 156, 453, 825, 1027, 1741, 2033
DraI TTTAAA 1 cut(s) 1420
DraIII CACNNNGTG 1 cut(s) 1943
DriI GACNNNNNGTC 2 cut(s) 625, 1444
EaeI YGGCCR 1 cut(s) 1777
Eam1104I CTCTTC 3 cut(s) 192, 410, 1332
Eam1105I GACNNNNNGTC 2 cut(s) 625, 1444
EarI CTCTTC 3 cut(s) 192, 410, 1332
Eco130I CCWWGG 6 cut(s) 14, 538, 995, 1044, 1348, 2039
Eco24I GRGCYC 2 cut(s) 1801, 2234
Eco32I GATATC 1 cut(s) 439
Eco47I GGWCC 4 cut(s) 739, 962, 1478, 2043
Eco57I CTGAAG 2 cut(s) 472, 1773
Eco88I CYCGRG 1 cut(s) 2126
Eco91I GGTNACC 1 cut(s) 1058
EcoNI CCTNNNNNAGG 1 cut(s) 863
EcoO109I RGGNCCY 4 cut(s) 739, 968, 1260, 1478
EcoO65I GGTNACC 1 cut(s) 1058
EcoRI GAATTC 1 cut(s) 930
EcoRII CCWGG 4 cut(s) 964, 1192, 1579, 1807
EcoRV GATATC 1 cut(s) 439
EcoT14I CCWWGG 6 cut(s) 14, 538, 995, 1044, 1348, 2039
EcoT38I GRGCYC 2 cut(s) 1801, 2234
ErhI CCWWGG 6 cut(s) 14, 538, 995, 1044, 1348, 2039
Esp3I CGTCTC 1 cut(s) 1131
FalI AAGNNNNNCTT 4 cut(s) 254, 286, 2126, 2158
FaqI GGGAC 7 cut(s) 158, 725, 736, 902, 1432, 1633, 1722
FauI CCCGC 1 cut(s) 670
FauNDI CATATG 2 cut(s) 1453, 1768
FblI GTMKAC 3 cut(s) 601, 840, 1172
FokI GGATG 6 cut(s) 93, 463, 656, 794, 845, 1447
FriOI GRGCYC 2 cut(s) 1801, 2234
FspBI CTAG 3 cut(s) 80, 351, 806
GlaI GCGC 1 cut(s) 993
GsaI CCCAGC 1 cut(s) 954
GsuI CTGGAG 2 cut(s) 483, 1155
HaeIII GGCC 7 cut(s) 472, 507, 969, 1262, 1552, 1574, 1779
HapII CCGG 5 cut(s) 110, 498, 1526, 1565, 1896
HgaI GACGC 1 cut(s) 1148
HhaI GCGC 1 cut(s) 994
Hin6I GCGC 1 cut(s) 992
HinP1I GCGC 1 cut(s) 992
HincII GTYRAC 1 cut(s) 841
HindII GTYRAC 1 cut(s) 841
HindIII AAGCTT 1 cut(s) 268
HinfI GANTC 7 cut(s) 458, 476, 638, 890, 1687, 1735, 2082
HpaII CCGG 5 cut(s) 110, 498, 1526, 1565, 1896
HphI GGTGA 4 cut(s) 629, 1228, 1667, 2028
Hpy166II GTNNAC 7 cut(s) 136, 602, 763, 841, 1173, 1859, 2046
Hpy188I TCNGA 5 cut(s) 312, 643, 781, 863, 936
Hpy188III TCNNGA 8 cut(s) 21, 110, 404, 462, 1031, 1739, 2152, 2227
Hpy8I GTNNAC 7 cut(s) 136, 602, 763, 841, 1173, 1859, 2046
Hpy99I CGWCG 2 cut(s) 962, 1169
HpyAV CCTTC 4 cut(s) 65, 1213, 1474, 1971
HpyCH4III ACNGT 5 cut(s) 427, 585, 962, 1446, 1871
HpyCH4IV ACGT 5 cut(s) 210, 411, 751, 1199, 1662
HpyF3I CTNAG 8 cut(s) 59, 309, 685, 870, 1428, 1615, 1733, 1940
HpySE526I ACGT 5 cut(s) 210, 411, 751, 1199, 1662
HspAI GCGC 1 cut(s) 992
KflI GGGWCCC 1 cut(s) 739
Kpn2I TCCGGA 1 cut(s) 109
Kzo9I GATC 6 cut(s) 156, 453, 825, 1027, 1741, 2033
LweI GCATC 5 cut(s) 484, 674, 811, 1607, 1813
MaeI CTAG 3 cut(s) 80, 351, 806
MaeII ACGT 5 cut(s) 210, 411, 751, 1199, 1662
MaeIII GTNAC 9 cut(s) 88, 617, 791, 1039, 1058, 1084, 1364, 1489, 2149
MalI GATC 6 cut(s) 158, 455, 827, 1029, 1743, 2035
MbiI CCGCTC 1 cut(s) 1709
MboI GATC 6 cut(s) 156, 453, 825, 1027, 1741, 2033
MboII GAAGA 5 cut(s) 179, 314, 427, 1258, 1319
MfeI CAATTG 3 cut(s) 1052, 1557, 1681
MhlI GDGCHC 6 cut(s) 583, 1801, 1861, 1902, 1939, 2234
MlsI TGGCCA 1 cut(s) 1779
MluNI TGGCCA 1 cut(s) 1779
MlyI GAGTC 6 cut(s) 452, 632, 884, 1681, 1744, 2076
MmeI TCCRAC 3 cut(s) 759, 1261, 2011
Mox20I TGGCCA 1 cut(s) 1779
MroI TCCGGA 1 cut(s) 109
MscI TGGCCA 1 cut(s) 1779
MseI TTAA 6 cut(s) 920, 1386, 1419, 1746, 2097, 2257
Msp20I TGGCCA 1 cut(s) 1779
MspA1I CMGCKG 1 cut(s) 677
MspI CCGG 5 cut(s) 110, 498, 1526, 1565, 1896
MspR9I CCNGG 8 cut(s) 499, 966, 1194, 1527, 1565, 1581, 1809, 1897
MunI CAATTG 3 cut(s) 1052, 1557, 1681
Mva1269I GAATGC 1 cut(s) 2106
MvaI CCWGG 4 cut(s) 966, 1194, 1581, 1809
MvnI CGCG 2 cut(s) 22, 830
NciI CCSGG 4 cut(s) 499, 1527, 1565, 1897
NcoI CCATGG 2 cut(s) 14, 995
NdeI CATATG 2 cut(s) 1453, 1768
NdeII GATC 6 cut(s) 156, 453, 825, 1027, 1741, 2033
NlaIV GGNNCC 9 cut(s) 740, 741, 907, 970, 1261, 1400, 1479, 1800, 2231
NmuCI GTSAC 5 cut(s) 88, 617, 791, 1489, 2149
NruI TCGCGA 1 cut(s) 22
NspI RCATGY 5 cut(s) 72, 202, 537, 1133, 1643
PagI TCATGA 1 cut(s) 403
PaqCI CACCTGC 1 cut(s) 1262
PciI ACATGT 1 cut(s) 68
PctI GAATGC 1 cut(s) 2106
PfeI GAWTC 1 cut(s) 476
PflMI CCANNNNNTGG 3 cut(s) 522, 1213, 1808
PfoI TCCNGGA 2 cut(s) 1525, 1579
Ple19I CGATCG 1 cut(s) 828
PleI GAGTC 6 cut(s) 452, 632, 884, 1681, 1743, 2076
PpsI GAGTC 6 cut(s) 452, 632, 884, 1681, 1743, 2076
Ppu21I YACGTR 1 cut(s) 752
PpuMI RGGWCCY 2 cut(s) 739, 1478
PscI ACATGT 1 cut(s) 68
Psp5II RGGWCCY 2 cut(s) 739, 1478
Psp6I CCWGG 4 cut(s) 964, 1192, 1579, 1807
PspEI GGTNACC 1 cut(s) 1058
PspFI CCCAGC 1 cut(s) 950
PspGI CCWGG 4 cut(s) 964, 1192, 1579, 1807
PspN4I GGNNCC 9 cut(s) 740, 741, 907, 970, 1261, 1400, 1479, 1800, 2231
PspPI GGNCC 7 cut(s) 506, 739, 962, 968, 1260, 1478, 2043
PspPPI RGGWCCY 2 cut(s) 739, 1478
PsrI GAACNNNNNNTAC 2 cut(s) 231, 263
PvuI CGATCG 1 cut(s) 828
RgaI GCGATCGC 1 cut(s) 828
RruI TCGCGA 1 cut(s) 22
RsaI GTAC 6 cut(s) 73, 137, 764, 1426, 1873, 2215
RsaNI GTAC 6 cut(s) 72, 136, 763, 1425, 1872, 2214
SalI GTCGAC 1 cut(s) 839
SaqAI TTAA 6 cut(s) 920, 1386, 1419, 1746, 2097, 2257
Sau3AI GATC 6 cut(s) 156, 453, 825, 1027, 1741, 2033
Sau96I GGNCC 7 cut(s) 506, 739, 962, 968, 1260, 1478, 2043
ScaI AGTACT 1 cut(s) 1426
SchI GAGTC 6 cut(s) 452, 632, 884, 1681, 1744, 2076
ScrFI CCNGG 8 cut(s) 499, 966, 1194, 1527, 1565, 1581, 1809, 1897
SduI GDGCHC 6 cut(s) 583, 1801, 1861, 1902, 1939, 2234
SfaAI GCGATCGC 1 cut(s) 828
SfaNI GCATC 5 cut(s) 484, 674, 811, 1607, 1813
SfcI CTRYAG 1 cut(s) 1867
SgfI GCGATCGC 1 cut(s) 828
SinI GGWCC 4 cut(s) 739, 962, 1478, 2043
SmlI CTYRAG 2 cut(s) 1103, 1505
SmoI CTYRAG 2 cut(s) 1103, 1505
SsiI CCGC 6 cut(s) 677, 758, 809, 830, 1707, 1722
SspI AATATT 1 cut(s) 229
SspMI CTAG 3 cut(s) 80, 351, 806
StyD4I CCNGG 8 cut(s) 497, 964, 1192, 1525, 1563, 1579, 1807, 1895
StyI CCWWGG 6 cut(s) 14, 538, 995, 1044, 1348, 2039
TaaI ACNGT 5 cut(s) 427, 585, 962, 1446, 1871
TaiI ACGT 5 cut(s) 213, 414, 754, 1202, 1665
TaqI TCGA 4 cut(s) 159, 456, 840, 2085
TatI WGTACW 4 cut(s) 71, 135, 762, 1424
TauI GCSGC 1 cut(s) 812
TfiI GAWTC 1 cut(s) 476
Tru1I TTAA 6 cut(s) 920, 1386, 1419, 1746, 2097, 2257
Tru9I TTAA 6 cut(s) 920, 1386, 1419, 1746, 2097, 2257
TscAI CASTG 9 cut(s) 430, 496, 667, 949, 1449, 1651, 1948, 2005, 2188
TseFI GTSAC 5 cut(s) 88, 617, 791, 1489, 2149
Tsp45I GTSAC 5 cut(s) 88, 617, 791, 1489, 2149
TspDTI ATGAA 6 cut(s) 179, 382, 392, 420, 1196, 2159
TspGWI ACGGA 1 cut(s) 1844
TspRI CASTG 9 cut(s) 430, 496, 667, 949, 1449, 1651, 1948, 2005, 2188
Van91I CCANNNNNTGG 3 cut(s) 522, 1213, 1808
VneI GTGCAC 1 cut(s) 1857
VpaK11BI GGWCC 4 cut(s) 739, 962, 1478, 2043
XagI CCTNNNNNAGG 1 cut(s) 863
XapI RAATTY 3 cut(s) 930, 1760, 1847
XceI RCATGY 5 cut(s) 72, 202, 537, 1133, 1643
XcmI CCANNNNNNNNNTGG 2 cut(s) 519, 1051
XmiI GTMKAC 3 cut(s) 601, 840, 1172
XspI CTAG 3 cut(s) 80, 351, 806
ZrmI AGTACT 1 cut(s) 1426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.