RLG00000030094

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000390
Physical Location & Seq
Forward (+)
55035320 .. 55039043
3724 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030094

Sequence Viewer

Length: 2220 bp
ATGGCTCTTCGATGGTTTCTCCTTCTCAGCCTCATTTGCAATATTCTACTGCTTGTCGATGTTACTCACTCAGCTGCTCAGGATACCCGAAAGGCTTATGTTGTGTATATGGGCGACAAGCCAAAGAACGAGGTACCCATATTACCTGATCTTCATGTGAATATGCTACAGGACGTAGTTGACAGTAGCAACATTAATATTGCACATGAAGCTCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATGACCGGAATGGATGGTGTAGTGTCTGTCTTCCCAAGCAAACAAAGGAGCGTCAAAACAACCAAGTCATGGAACTTCCTTGGGTTTCCAGAAACAATTAAGAGAAGCGCCCTTGAAAGGGATATCATCGTTGGTGTGATGGACAGTGGAATTTGGCCAGAGTCGGACAGCTTTAGTGATGCTGATTTTGGTCCTCCACCTAAGAAATGGAAAGGCAAATGTCAAGGCAATGGAAATCTTACTTGTAACAATAAAATCATTGGAGCACAGTATTACCGTGCTTCTCGATCCTTCGAAGAAGATGTTGAGTCTCCAAGAGACTCAAATGGTCATGGAACTCATACTGCATCAATAGCAGCTGGGAACACAGTGAGCAAGGCAAGCCTAAATGGTTTAGGGTTGGGAAAAGCAAGAGGAGGGGTGCCATCAGCACGTATTGCTGTGTACAAAGTATGCTGGAGCAGCACGCCTGCTTGTGAAGATGTTGACGTTTTAGCGGCATTCGATGATGCCATTGCTGACGGTGTCGACATTCTTTCTATTTCCGTCGGGGGCAGTATTCCAGTTGATTATTTAAATGATGCGTTTGCAATTGGGTCATTTCACGCTACCAGAAATGGGATATTAGTTTCCATGGCCGCTGGTAATGAAGGTCCGGGGGCAAAAACTGTGACAAACTTTGCACCATGGCAACTTTCTGTGGCTGGTACCACAATAAACCGCCAATTCATCACCAAGGTTCAATTGGGTAACAGTAAAATCTATGAGGGACTTTTACCAAACATATATGACCTCCGGGGTAAATTATATCCTTTAATTTATGGTGGAGACGCACCCAATAAAAAAGCAGGTGCTAACGGGGCCCTCTCTAGGTTTTGTCTCCAAGATACCTTAGAAGAAGATTTAATCAAAGATAAAATTGTGCTTTGTGATGGCCCTATCAATGGGGCTGGGGGCGGGGCCATATTGGGTGGTGCAGTCGGAATTGTTCTGACGGGACAAAAAGTCACTGATGAAGTGCTTGGCCCTCTTCCCGTGCCTGCATCACACGTCGGGCTGGAAGAACATAGCGACATTTACAAATACATAAACTTAACAAGGAACCCAACTGCAACTATTTGGAAAAGTGAAGAGATTAATGATGTATTGGCTCCATACGTGCCCTCCTACTCATCAAGGGGTCCAAATCCGATCAATCCCAACATTCTCAAGCCAGATTTAGCAGCTCCAGGAACTTACATTCTAGCAGCATATCCCCCAAGTGGTGGTGGCAGTAGAGCTGGATACTATTTAGAAACCGGGACATCAATGGCGTGCCCCCATGCTACAGCGGTAGCTGCATACGTCAAATCATTTCACCCTAAATGGTCACCGGCTGCTATCCAATCGGCTCTCATCACTACCGCTAAATCTATGAGTGCCAAAACTAGCCCTGATGCTGAATTTGCATACGGAGCTGGCCTGATAAATCCTTCTAGGGCTCCATATCCTGGTTTGGTGTACAATCTTGATGAACAAGACTACATAGATTTTTTGTGTTCACAAGGATACAGTGGTAAACTATTGAAAGCCATAACCGGAGACAAGACTAGTTGTTCATCAAAATCTAATAATGGAACAACCAATGACCTGAACTATCCTTCTTTTGTACTTTCCATCAAGGATCCGAAATTTGTTAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCGTCGAATTCCACATACAGAGTTACAGTGGTGGCTCCATTGGGACTCAAAATCAATGTGAATCCAAATGTGCTATCGTTCACATCTCTCGGGCAAAAGAAATCTTTTGTTGTCACTATAAAAGGGCCGATTGAGAAATCAAATTTAGTCTCTGCGTCTTTGATGTGGGATGATGGTGTTTTCCAAGTCAGGAGCCCCATCGTTGTCTATGTTGCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

740

Amino Acids

78.94

Weight (kDa)

7.54

Isoelectric Point (pI)

35.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 112 6.3e-14 Peptidase inhibitor I9
Peptidase_S8 PF00082 135 - 582 5.3e-43 Subtilase family
fn3_6 PF17766 638 - 735 2.6e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1124
Acc36I ACCTGC 1 cut(s) 1124
Acc65I GGTACC 2 cut(s) 133, 992
AccB1I GGYRCC 3 cut(s) 133, 706, 992
AccB7I CCANNNNNTGG 3 cut(s) 354, 1550, 1775
AccI GTMKAC 1 cut(s) 813
AciI CCGC 6 cut(s) 782, 924, 1006, 1242, 1616, 1689
AclWI GGATC 4 cut(s) 567, 1943, 1956, 2005
AcoI YGGCCR 2 cut(s) 440, 921
AcsI RAATTY 5 cut(s) 435, 1727, 1955, 2005, 2140
AfaI GTAC 5 cut(s) 135, 731, 994, 1787, 1935
AfiI CCNNNNNNNGG 9 cut(s) 354, 402, 403, 903, 1155, 1229, 1547, 1550, 1775
AflIII ACRYGT 1 cut(s) 1333
AgsI TTSAA 4 cut(s) 244, 401, 1028, 1852
AhdI GACNNNNNGTC 1 cut(s) 1289
AhlI ACTAGT 1 cut(s) 1874
AjiI CACGTC 1 cut(s) 1336
AjnI CCWGG 2 cut(s) 1513, 1774
AluBI AGCT 9 cut(s) 74, 212, 228, 456, 644, 1511, 1565, 1622, 1742
AluI AGCT 9 cut(s) 74, 212, 228, 456, 644, 1511, 1565, 1622, 1742
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 6 cut(s) 597, 600, 1107, 1169, 1860, 2152
AlwI GGATC 4 cut(s) 567, 1943, 1956, 2005
Ama87I CYCGRG 1 cut(s) 2087
AoxI GGCC 8 cut(s) 440, 921, 1146, 1219, 1245, 1309, 1744, 2123
ApaI GGGCCC 1 cut(s) 1150
ApeKI GCWGC 8 cut(s) 74, 254, 641, 747, 1508, 1532, 1622, 1661
ApoI RAATTY 5 cut(s) 435, 1727, 1955, 2005, 2140
AseI ATTAAT 2 cut(s) 195, 1422
Asp718I GGTACC 2 cut(s) 133, 992
AspLEI GCGC 1 cut(s) 395
AspS9I GGNCC 9 cut(s) 476, 938, 1146, 1147, 1220, 1245, 1310, 1466, 2123
AsuC2I CCSGG 3 cut(s) 942, 1082, 1585
AsuHPI GGTGA 3 cut(s) 1009, 1634, 1647
AsuII TTCGAA 1 cut(s) 579
AvaI CYCGRG 1 cut(s) 2087
AvaII GGWCC 3 cut(s) 476, 938, 1466
BaeGI GKGCMC 3 cut(s) 1150, 1449, 1604
BaeI ACNNNNGTAYC 2 cut(s) 1825, 1858
BalI TGGCCA 1 cut(s) 442
BamHI GGATCC 1 cut(s) 1948
BanI GGYRCC 3 cut(s) 133, 706, 992
BanII GRGCYC 3 cut(s) 1150, 1768, 2195
BbsI GAAGAC 2 cut(s) 307, 1963
Bbv12I GWGCWC 1 cut(s) 553
BbvI GCAGC 8 cut(s) 61, 241, 653, 759, 1520, 1544, 1609, 1648
BccI CCATC 8 cut(s) 6, 293, 418, 718, 1211, 1949, 2165, 2204
BciT130I CCWGG 2 cut(s) 1515, 1776
BciVI GTATCC 3 cut(s) 76, 1562, 1826
BcnI CCSGG 3 cut(s) 942, 1082, 1585
BcoDI GTCTC 6 cut(s) 597, 600, 1107, 1169, 1860, 2152
BcuI ACTAGT 1 cut(s) 1874
BfaI CTAG 5 cut(s) 1155, 1529, 1713, 1761, 1875
BfmI CTRYAG 2 cut(s) 167, 1611
BfoI RGCGCY 1 cut(s) 396
BfuAI ACCTGC 1 cut(s) 1124
BfuI GTATCC 3 cut(s) 76, 1562, 1826
Bme1390I CCNGG 5 cut(s) 942, 1082, 1515, 1585, 1776
Bme18I GGWCC 3 cut(s) 476, 938, 1466
BmeRI GACNNNNNGTC 1 cut(s) 1289
BmeT110I CYCGRG 1 cut(s) 2087
BmgBI CACGTC 1 cut(s) 1336
BmgT120I GGNCC 9 cut(s) 476, 938, 1146, 1147, 1220, 1245, 1310, 1466, 2123
BmrFI CCNGG 5 cut(s) 942, 1082, 1515, 1585, 1776
BmsI GCATC 6 cut(s) 454, 641, 784, 856, 1337, 1711
BpiI GAAGAC 2 cut(s) 307, 1963
BpmI CTGGAG 2 cut(s) 763, 1497
Bpu10I CCTNAGC 1 cut(s) 78
Bpu14I TTCGAA 1 cut(s) 579
BpuEI CTTGAG 1 cut(s) 1478
BpuMI CCSGG 3 cut(s) 942, 1082, 1585
BsaAI YACGTR 2 cut(s) 719, 1444
BsaJI CCNNGG 6 cut(s) 364, 918, 941, 971, 1020, 1081
BsaWI WCCGGW 2 cut(s) 290, 1862
BsaXI ACNNNNNCTCC 2 cut(s) 1433, 1463
Bsc4I CCNNNNNNNGG 9 cut(s) 354, 402, 403, 903, 1155, 1229, 1547, 1550, 1775
Bse118I RCCGGY 1 cut(s) 1657
Bse1I ACTGG 1 cut(s) 848
Bse3DI GCAATG 3 cut(s) 264, 520, 798
BseBI CCWGG 2 cut(s) 1515, 1776
BseDI CCNNGG 6 cut(s) 364, 918, 941, 971, 1020, 1081
BseGI GGATG 2 cut(s) 304, 2173
BseLI CCNNNNNNNGG 9 cut(s) 354, 402, 403, 903, 1155, 1229, 1547, 1550, 1775
BseMI GCAATG 3 cut(s) 264, 520, 798
BseMII CTCAG 3 cut(s) 40, 84, 92
BseNI ACTGG 1 cut(s) 848
BseRI GAGGAG 1 cut(s) 714
BseSI GKGCMC 3 cut(s) 1150, 1449, 1604
BseXI GCAGC 8 cut(s) 61, 241, 653, 759, 1520, 1544, 1609, 1648
BseYI CCCAGC 2 cut(s) 644, 1235
BsgI GTGCAG 1 cut(s) 1281
BshFI GGCC 8 cut(s) 442, 923, 1148, 1221, 1247, 1311, 1746, 2125
BshNI GGYRCC 3 cut(s) 133, 706, 992
BsiHKAI GWGCWC 1 cut(s) 553
BsiHKCI CYCGRG 1 cut(s) 2087
BsiSI CCGG 6 cut(s) 291, 941, 1081, 1584, 1658, 1863
BslFI GGGAC 4 cut(s) 1068, 1296, 1600, 2055
BslI CCNNNNNNNGG 9 cut(s) 354, 402, 403, 903, 1155, 1229, 1547, 1550, 1775
BsmAI GTCTC 6 cut(s) 597, 600, 1107, 1169, 1860, 2152
BsmBI CGTCTC 1 cut(s) 1107
BsmFI GGGAC 4 cut(s) 1068, 1296, 1600, 2055
BsmI GAATGC 1 cut(s) 785
BsnI GGCC 8 cut(s) 442, 923, 1148, 1221, 1247, 1311, 1746, 2125
BsoBI CYCGRG 1 cut(s) 2087
Bsp119I TTCGAA 1 cut(s) 579
Bsp120I GGGCCC 1 cut(s) 1146
Bsp1286I GDGCHC 6 cut(s) 553, 1150, 1449, 1604, 1768, 2195
Bsp1407I TGTACA 2 cut(s) 729, 1785
Bsp143I GATC 5 cut(s) 148, 572, 1476, 1948, 1997
Bsp19I CCATGG 2 cut(s) 918, 971
BspACI CCGC 6 cut(s) 782, 924, 1006, 1242, 1616, 1689
BspANI GGCC 8 cut(s) 442, 923, 1148, 1221, 1247, 1311, 1746, 2125
BspCNI CTCAG 3 cut(s) 39, 83, 91
BspMI ACCTGC 1 cut(s) 1124
BspPI GGATC 4 cut(s) 567, 1943, 1956, 2005
BspQI GCTCTTC 1 cut(s) 12
BspT104I TTCGAA 1 cut(s) 579
BspT107I GGYRCC 3 cut(s) 133, 706, 992
BsrDI GCAATG 3 cut(s) 264, 520, 798
BsrFI RCCGGY 1 cut(s) 1657
BsrGI TGTACA 2 cut(s) 729, 1785
BsrI ACTGG 1 cut(s) 848
BssAI RCCGGY 1 cut(s) 1657
BssECI CCNNGG 6 cut(s) 364, 918, 941, 971, 1020, 1081
BssMI GATC 5 cut(s) 148, 572, 1476, 1948, 1997
BssT1I CCWWGG 4 cut(s) 364, 918, 971, 1020
Bst2UI CCWGG 2 cut(s) 1515, 1776
Bst6I CTCTTC 3 cut(s) 12, 1320, 1410
BstAPI GCANNNNNTGC 1 cut(s) 722
BstAUI TGTACA 2 cut(s) 729, 1785
BstBAI YACGTR 2 cut(s) 719, 1444
BstBI TTCGAA 1 cut(s) 579
BstC8I GCNNGC 6 cut(s) 667, 752, 756, 1326, 1600, 1744
BstDEI CTNAG 5 cut(s) 26, 70, 78, 486, 1177
BstDSI CCRYGG 2 cut(s) 918, 971
BstEII GGTNACC 1 cut(s) 1653
BstF5I GGATG 2 cut(s) 304, 2173
BstH2I RGCGCY 1 cut(s) 396
BstHHI GCGC 1 cut(s) 395
BstKTI GATC 5 cut(s) 151, 575, 1479, 1951, 2000
BstMAI GTCTC 6 cut(s) 597, 600, 1107, 1169, 1860, 2152
BstMBI GATC 5 cut(s) 148, 572, 1476, 1948, 1997
BstNI CCWGG 2 cut(s) 1515, 1776
BstPI GGTNACC 1 cut(s) 1653
BstSCI CCNGG 5 cut(s) 940, 1080, 1513, 1583, 1774
BstSFI CTRYAG 2 cut(s) 167, 1611
BstSLI GKGCMC 3 cut(s) 1150, 1449, 1604
BstV1I GCAGC 8 cut(s) 61, 241, 653, 759, 1520, 1544, 1609, 1648
BstV2I GAAGAC 2 cut(s) 307, 1963
BstX2I RGATCY 1 cut(s) 1948
BstYI RGATCY 1 cut(s) 1948
BsuI GTATCC 3 cut(s) 76, 1562, 1826
BsuRI GGCC 8 cut(s) 442, 923, 1148, 1221, 1247, 1311, 1746, 2125
BtgI CCRYGG 2 cut(s) 918, 971
BtrI CACGTC 1 cut(s) 1336
BtsCI GGATG 2 cut(s) 304, 2173
BtsIMutI CAGTG 5 cut(s) 436, 660, 1293, 1843, 2031
BveI ACCTGC 1 cut(s) 1124
Cac8I GCNNGC 6 cut(s) 667, 752, 756, 1326, 1600, 1744
CfoI GCGC 1 cut(s) 395
Cfr10I RCCGGY 1 cut(s) 1657
Cfr13I GGNCC 9 cut(s) 476, 938, 1146, 1147, 1220, 1245, 1310, 1466, 2123
CseI GACGC 3 cut(s) 325, 1124, 2142
Csp6I GTAC 5 cut(s) 134, 730, 993, 1786, 1934
CviAII CATG 7 cut(s) 155, 206, 354, 617, 919, 972, 1607
CviQI GTAC 5 cut(s) 134, 730, 993, 1786, 1934
DdeI CTNAG 5 cut(s) 26, 70, 78, 486, 1177
DpnI GATC 5 cut(s) 150, 574, 1478, 1950, 1999
DpnII GATC 5 cut(s) 148, 572, 1476, 1948, 1997
DraI TTTAAA 1 cut(s) 861
DriI GACNNNNNGTC 1 cut(s) 1289
EaeI YGGCCR 2 cut(s) 440, 921
Eam1104I CTCTTC 3 cut(s) 12, 1320, 1410
Eam1105I GACNNNNNGTC 1 cut(s) 1289
EarI CTCTTC 3 cut(s) 12, 1320, 1410
Eco130I CCWWGG 4 cut(s) 364, 918, 971, 1020
Eco24I GRGCYC 3 cut(s) 1150, 1768, 2195
Eco32I GATATC 1 cut(s) 409
Eco47I GGWCC 3 cut(s) 476, 938, 1466
Eco88I CYCGRG 1 cut(s) 2087
Eco91I GGTNACC 1 cut(s) 1653
EcoO109I RGGNCCY 2 cut(s) 1146, 1147
EcoO65I GGTNACC 1 cut(s) 1653
EcoRI GAATTC 1 cut(s) 2005
EcoRII CCWGG 2 cut(s) 1513, 1774
EcoRV GATATC 1 cut(s) 409
EcoT14I CCWWGG 4 cut(s) 364, 918, 971, 1020
EcoT38I GRGCYC 3 cut(s) 1150, 1768, 2195
ErhI CCWWGG 4 cut(s) 364, 918, 971, 1020
Esp3I CGTCTC 1 cut(s) 1107
FaeI CATG 7 cut(s) 158, 209, 357, 620, 922, 975, 1610
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FaqI GGGAC 4 cut(s) 1068, 1296, 1600, 2055
FatI CATG 7 cut(s) 154, 205, 353, 616, 918, 971, 1606
FauI CCCGC 1 cut(s) 1235
FblI GTMKAC 1 cut(s) 813
FokI GGATG 2 cut(s) 311, 2180
FriOI GRGCYC 3 cut(s) 1150, 1768, 2195
FspBI CTAG 5 cut(s) 1155, 1529, 1713, 1761, 1875
GlaI GCGC 1 cut(s) 394
GsaI CCCAGC 2 cut(s) 648, 1239
GsuI CTGGAG 2 cut(s) 763, 1497
HaeII RGCGCY 1 cut(s) 396
HaeIII GGCC 8 cut(s) 442, 923, 1148, 1221, 1247, 1311, 1746, 2125
HapII CCGG 6 cut(s) 291, 941, 1081, 1584, 1658, 1863
HgaI GACGC 3 cut(s) 325, 1124, 2142
HhaI GCGC 1 cut(s) 395
Hin1II CATG 7 cut(s) 158, 209, 357, 620, 922, 975, 1610
Hin6I GCGC 1 cut(s) 393
HinP1I GCGC 1 cut(s) 393
HincII GTYRAC 3 cut(s) 181, 772, 814
HindII GTYRAC 3 cut(s) 181, 772, 814
HinfI GANTC 5 cut(s) 446, 593, 605, 2043, 2059
HpaII CCGG 6 cut(s) 291, 941, 1081, 1584, 1658, 1863
HphI GGTGA 3 cut(s) 1009, 1634, 1647
Hpy166II GTNNAC 8 cut(s) 181, 730, 772, 814, 1786, 1826, 1844, 2079
Hpy188I TCNGA 5 cut(s) 451, 1268, 1278, 1476, 1953
Hpy188III TCNNGA 5 cut(s) 80, 374, 570, 1793, 2188
Hpy8I GTNNAC 8 cut(s) 181, 730, 772, 814, 1786, 1826, 1844, 2079
Hpy99I CGWCG 3 cut(s) 836, 1340, 2005
HpyAV CCTTC 5 cut(s) 32, 586, 929, 1767, 1935
HpyCH4IV ACGT 6 cut(s) 174, 718, 774, 1335, 1443, 1629
HpyF3I CTNAG 5 cut(s) 26, 70, 78, 486, 1177
HpySE526I ACGT 6 cut(s) 174, 718, 774, 1335, 1443, 1629
Hsp92II CATG 7 cut(s) 158, 209, 357, 620, 922, 975, 1610
HspAI GCGC 1 cut(s) 393
KpnI GGTACC 2 cut(s) 137, 996
Kzo9I GATC 5 cut(s) 148, 572, 1476, 1948, 1997
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 9 cut(s) 333, 548, 744, 1441, 1516, 1739, 1771, 2038, 2190
Lsp1109I GCAGC 8 cut(s) 61, 241, 653, 759, 1520, 1544, 1609, 1648
LweI GCATC 6 cut(s) 454, 641, 784, 856, 1337, 1711
MaeI CTAG 5 cut(s) 1155, 1529, 1713, 1761, 1875
MaeII ACGT 6 cut(s) 174, 718, 774, 1335, 1443, 1629
MaeIII GTNAC 9 cut(s) 61, 530, 955, 1034, 1291, 1653, 1984, 2020, 2110
MalI GATC 5 cut(s) 150, 574, 1478, 1950, 1999
MboI GATC 5 cut(s) 148, 572, 1476, 1948, 1997
MfeI CAATTG 2 cut(s) 876, 1028
MflI RGATCY 1 cut(s) 1948
MhlI GDGCHC 6 cut(s) 553, 1150, 1449, 1604, 1768, 2195
MlsI TGGCCA 1 cut(s) 442
MluNI TGGCCA 1 cut(s) 442
MlyI GAGTC 4 cut(s) 455, 599, 602, 2037
MmeI TCCRAC 3 cut(s) 429, 1246, 1975
Mox20I TGGCCA 1 cut(s) 442
MscI TGGCCA 1 cut(s) 442
MseI TTAA 9 cut(s) 195, 384, 860, 1100, 1190, 1379, 1422, 1962, 2218
Msp20I TGGCCA 1 cut(s) 442
MspA1I CMGCKG 4 cut(s) 74, 644, 926, 1616
MspI CCGG 6 cut(s) 291, 941, 1081, 1584, 1658, 1863
MspR9I CCNGG 5 cut(s) 942, 1082, 1515, 1585, 1776
MunI CAATTG 2 cut(s) 876, 1028
Mva1269I GAATGC 1 cut(s) 785
MvaI CCWGG 2 cut(s) 1515, 1776
NciI CCSGG 3 cut(s) 942, 1082, 1585
NcoI CCATGG 2 cut(s) 918, 971
NdeII GATC 5 cut(s) 148, 572, 1476, 1948, 1997
NlaIII CATG 7 cut(s) 158, 209, 357, 620, 922, 975, 1610
NmuCI GTSAC 5 cut(s) 955, 1291, 1653, 1984, 2110
NspV TTCGAA 1 cut(s) 579
PaqCI CACCTGC 1 cut(s) 1124
PciSI GCTCTTC 1 cut(s) 12
PctI GAATGC 1 cut(s) 785
PfeI GAWTC 1 cut(s) 2059
PflFI GACNNNGTC 1 cut(s) 809
PflMI CCANNNNNTGG 3 cut(s) 354, 1550, 1775
PfoI TCCNGGA 1 cut(s) 1513
PleI GAGTC 4 cut(s) 454, 599, 601, 2037
PpsI GAGTC 4 cut(s) 454, 599, 601, 2037
Ppu21I YACGTR 2 cut(s) 719, 1444
PshBI ATTAAT 2 cut(s) 195, 1422
Psp6I CCWGG 2 cut(s) 1513, 1774
PspEI GGTNACC 1 cut(s) 1653
PspFI CCCAGC 2 cut(s) 644, 1235
PspGI CCWGG 2 cut(s) 1513, 1774
PspOMI GGGCCC 1 cut(s) 1146
PspPI GGNCC 9 cut(s) 476, 938, 1146, 1147, 1220, 1245, 1310, 1466, 2123
PsuI RGATCY 1 cut(s) 1948
PsyI GACNNNGTC 1 cut(s) 809
PvuII CAGCTG 2 cut(s) 74, 644
RsaI GTAC 5 cut(s) 135, 731, 994, 1787, 1935
RsaNI GTAC 5 cut(s) 134, 730, 993, 1786, 1934
SalI GTCGAC 1 cut(s) 812
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 9 cut(s) 195, 384, 860, 1100, 1190, 1379, 1422, 1962, 2218
Sau3AI GATC 5 cut(s) 148, 572, 1476, 1948, 1997
Sau96I GGNCC 9 cut(s) 476, 938, 1146, 1147, 1220, 1245, 1310, 1466, 2123
SchI GAGTC 4 cut(s) 455, 599, 602, 2037
ScrFI CCNGG 5 cut(s) 942, 1082, 1515, 1585, 1776
SduI GDGCHC 6 cut(s) 553, 1150, 1449, 1604, 1768, 2195
SfaNI GCATC 6 cut(s) 454, 641, 784, 856, 1337, 1711
SfcI CTRYAG 2 cut(s) 167, 1611
SfuI TTCGAA 1 cut(s) 579
SinI GGWCC 3 cut(s) 476, 938, 1466
SmiI ATTTAAAT 1 cut(s) 861
SmlI CTYRAG 1 cut(s) 1493
SmoI CTYRAG 1 cut(s) 1493
SpeI ACTAGT 1 cut(s) 1874
SsiI CCGC 6 cut(s) 782, 924, 1006, 1242, 1616, 1689
SspI AATATT 2 cut(s) 43, 199
SspMI CTAG 5 cut(s) 1155, 1529, 1713, 1761, 1875
StyD4I CCNGG 5 cut(s) 940, 1080, 1513, 1583, 1774
StyI CCWWGG 4 cut(s) 364, 918, 971, 1020
SwaI ATTTAAAT 1 cut(s) 861
TaiI ACGT 6 cut(s) 177, 721, 777, 1338, 1446, 1632
TaqI TCGA 7 cut(s) 10, 57, 571, 579, 789, 813, 2003
TatI WGTACW 3 cut(s) 729, 1785, 1933
TauI GCSGC 2 cut(s) 785, 926
TfiI GAWTC 1 cut(s) 2059
Tru1I TTAA 9 cut(s) 195, 384, 860, 1100, 1190, 1379, 1422, 1962, 2218
Tru9I TTAA 9 cut(s) 195, 384, 860, 1100, 1190, 1379, 1422, 1962, 2218
TscAI CASTG 5 cut(s) 436, 660, 1300, 1843, 2031
TseFI GTSAC 5 cut(s) 955, 1291, 1653, 1984, 2110
TseI GCWGC 8 cut(s) 74, 254, 641, 747, 1508, 1532, 1622, 1661
Tsp45I GTSAC 5 cut(s) 955, 1291, 1653, 1984, 2110
TspDTI ATGAA 7 cut(s) 143, 222, 948, 1003, 1314, 1812, 1872
TspGWI ACGGA 2 cut(s) 820, 1752
TspRI CASTG 5 cut(s) 436, 660, 1300, 1843, 2031
Tth111I GACNNNGTC 1 cut(s) 809
Van91I CCANNNNNTGG 3 cut(s) 354, 1550, 1775
VpaK11BI GGWCC 3 cut(s) 476, 938, 1466
VspI ATTAAT 2 cut(s) 195, 1422
XapI RAATTY 5 cut(s) 435, 1727, 1955, 2005, 2140
XcmI CCANNNNNNNNNTGG 2 cut(s) 489, 1027
XmiI GTMKAC 1 cut(s) 813
XspI CTAG 5 cut(s) 1155, 1529, 1713, 1761, 1875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.