Rroxscaffold_4G00286760

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000390
Physical Location & Seq
Forward (+)
7744931 .. 7749601
4671 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00286760.1

Sequence Viewer

Length: 2286 bp
ATGAGAGCAATGCCTCTAGCTAGTAGCACCATACAGAGTAATAGTATGGCTCTTCGATGGTTTCTCCTTCTCAACCTCATTTGCAGTATTCTACTTCTTGTCGATGTTACTCATTCAGCTGCTCAGGATGCCCGAAAGGCTTATGTTGTGTATATGGGCGGCAAGCCAAAGAATGGGGTACCCATATTACCTGATCTTCATGTGAATATGCTACAAGACGTAGTTGACAGTAGCAACATTGATGTTGCACATGAAGATCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATGATTGAACTGGCTAAACGAAAACGGAAGAAGAAGAAAGATGAAGACCATGAAGAAAGGTTATCGAGGAGCGTCAAAACAACAAAGTCATGGAACTTTCTTGGGTTTCCAGAAACAGTTAAGAGAGGCGTCCTTGAAAGGGATATCATTGTTGGTGTGATAGACAGTGGAATTTGGCCAGAGTCGGACAGCTTTAGTGATACTGGTTTTGGTCCCCCACCCAAGAAATGGAAAGGCAAATGTCAAGGCAATGGAAATCTTACTTGTAACAATAAAATCATCGGAGCACAATATTATCGTGCTTCTCAATCATTCAAAGAAGATGTTGAGTCTCCAAGAGACTCAAACGGTCATGGAACTCACACCGCATCAACAGCAGCTGGGAACACAGTGCTCAAGGCAAGCTTTAATGGTTTAGGGTCGGGAACAGCAAGAGGAGGGGTGCCATCAGCGCGTATTGCTGTGTACAAAGCATGTTGGGGCAGCAGTGGCAGTTGTGACGATGCTGATATTCTAGCCGCATTTGATGATGCCATTGCTGACGGTGTAGACATTCTCTCTGTTTCCGTCGGGGGCAATATTCCACTTGATTATTTGAATGATTCATTCGCAATTGGGTCATTTCACGCTACCAGAAATGGGATATTAGTTTCCATGGCCGCTGGTAATGAAGGTCCGGGCGCAAAAACTGTGACAAACTTTGCACCATGGCAACTTTCTGTGGCTGCTACCACCATAAATCGCCAATTCATCACCAAGGTTCAATTGGGTAACGGTAAAATCTATGAGGGACTTTTACCAAACATATATGACCTCCGGGGTAAATTCTATCCTTTAATTTATGGTGGAGACGCGCCCAATACAAAAGCAGGTGCTGACGGGAGTATCTCTAGGTTTTGTGTACGAGATGCCTTAGAAGAAGATTTGATCAAAGGTAAAATTGTGTTGTGTGATGGCCCTATGGATGAGGCTGGGGTTGGGGCCATATTGGGTGGTGCAGCCGGAATTGTTCTGACGGGAAAAAAAGTCGACGATGAACTGTTTAGCCTTTATCCCGTGCCTGCATCTTTCGTTGGGTTGGAAGAAAACAGCGACATTTACAAATACATAAATTCAACAAGGAACGCAACTGCAACTATATGGAAAAGTGAAGAGATTAGTGATGTATTGGCTCCATACGTGCCCTCCTACTCATCGAGGGGCCCAAATCCGATCAATCCCAACATTCTCAAGCCAGATTTAGCTGCTCCAGGAACTTACATTCTGGCAGCATATCCCCCAAGCGGTGGTGGGGGTAGAGGCAGATACTATATGGACACCGGGACATCAATGGCATGCCCCCATGCTACGGCCACAGCTGCATACGTCAAATCATTTCACCCTAAATGGTCAGCGGCTGCTATCCAATCGGCTCTCATCACTACTGCTAAACCTATGAATGCCACAACTAGCCCTGACGCTGAATTTGCATACGGAGCTGGCCTAATAAACCCTTCTAGGGCGCCATATCCTGGTTTGGTGTATGATCTTGATGAACAAGACTACATAGATTTTTTGTGTTCACAAGGATACAGTGGTAAACTATTGCAAGCCATAACCAGGGACAAAACTAGTTGCTCATCAAAATCTATTAATAGAACAGCTAATGACATGAACTATCCTTCTTTTGCACTTTCCATCAAGGATCCGAAATTTGTCAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCGTCAAAATCCACATACAGAGCTAAAACGGTGGCTCCATTGGGACTCAAAATCAATGTGAATCCAAGTGTGCTATCGTTCACATCTCTCGGGCAAAAGAAATCTTTTGTTGTCACTATAAAAGGGCCGATTGAGAAATCAAATCTGGTCTCTGCATCTTTGGTGTGGGATGATGGTGCTTTCCAAGTCAGGAGTCCCATTGTTGTCTATGTTGCTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

761

Amino Acids

81.63

Weight (kDa)

8.53

Isoelectric Point (pI)

35.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 48 - 111 1e-07 Peptidase inhibitor I9
Peptidase_S8 PF00082 157 - 604 5.7e-43 Subtilase family
fn3_6 PF17766 660 - 757 8e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1190
Acc36I ACCTGC 1 cut(s) 1190
Acc65I GGTACC 1 cut(s) 178
AccB1I GGYRCC 3 cut(s) 178, 772, 1831
AccB7I CCANNNNNTGG 4 cut(s) 173, 558, 1616, 1841
AccI GTMKAC 2 cut(s) 879, 1359
AccII CGCG 2 cut(s) 784, 1184
AciI CCGC 6 cut(s) 159, 696, 849, 990, 1614, 1724
AclWI GGATC 3 cut(s) 2009, 2022, 2071
AcoI YGGCCR 3 cut(s) 506, 987, 1680
AcsI RAATTY 5 cut(s) 501, 1154, 1441, 1793, 2021
AcyI GRCGYC 2 cut(s) 459, 1832
AfaI GTAC 3 cut(s) 180, 797, 1233
AgsI TTSAA 7 cut(s) 289, 338, 467, 646, 928, 1094, 1446
AhlI ACTAGT 1 cut(s) 1940
AjnI CCWGG 3 cut(s) 1579, 1840, 1928
Alw21I GWGCWC 2 cut(s) 619, 726
Alw26I GTCTC 4 cut(s) 663, 666, 1173, 2218
AlwI GGATC 3 cut(s) 2009, 2022, 2071
AlwNI CAGNNNCTG 3 cut(s) 710, 1205, 1727
Ama87I CYCGRG 1 cut(s) 2153
AoxI GGCC 8 cut(s) 506, 987, 1285, 1311, 1531, 1680, 1810, 2189
ApaI GGGCCC 1 cut(s) 1535
ApoI RAATTY 5 cut(s) 501, 1154, 1441, 1793, 2021
ArsI GACNNNNNNTTYG 2 cut(s) 1778, 1810
AseI ATTAAT 1 cut(s) 1962
Asp718I GGTACC 1 cut(s) 178
AspLEI GCGC 4 cut(s) 784, 1013, 1186, 1834
AspS9I GGNCC 7 cut(s) 542, 1004, 1286, 1311, 1531, 1532, 2189
AsuC2I CCSGG 3 cut(s) 1008, 1148, 1651
AsuHPI GGTGA 2 cut(s) 1075, 1700
AvaI CYCGRG 1 cut(s) 2153
AvaII GGWCC 2 cut(s) 542, 1004
BaeGI GKGCMC 2 cut(s) 1515, 1535
BaeI ACNNNNGTAYC 4 cut(s) 1198, 1231, 1891, 1924
BalI TGGCCA 1 cut(s) 508
BamHI GGATCC 1 cut(s) 2014
BanI GGYRCC 3 cut(s) 178, 772, 1831
BanII GRGCYC 1 cut(s) 1535
BbsI GAAGAC 2 cut(s) 381, 2029
Bbv12I GWGCWC 2 cut(s) 619, 726
BccI CCATC 5 cut(s) 51, 784, 1277, 2015, 2231
BceAI ACGGC 1 cut(s) 1695
BciT130I CCWGG 3 cut(s) 1581, 1842, 1930
BciVI GTATCC 1 cut(s) 1892
BclI TGATCA 1 cut(s) 1257
BcnI CCSGG 3 cut(s) 1008, 1148, 1651
BcoDI GTCTC 4 cut(s) 663, 666, 1173, 2218
BcuI ACTAGT 1 cut(s) 1940
BfaI CTAG 7 cut(s) 17, 21, 845, 1221, 1779, 1827, 1941
BfoI RGCGCY 1 cut(s) 1835
BfuAI ACCTGC 1 cut(s) 1190
BfuI GTATCC 1 cut(s) 1892
BglI GCCNNNNNGGC 1 cut(s) 137
BglII AGATCT 1 cut(s) 256
Bme1390I CCNGG 6 cut(s) 1008, 1148, 1581, 1651, 1842, 1930
Bme18I GGWCC 2 cut(s) 542, 1004
BmeT110I CYCGRG 1 cut(s) 2153
BmgT120I GGNCC 7 cut(s) 542, 1004, 1286, 1311, 1531, 1532, 2189
BmrFI CCNGG 6 cut(s) 1008, 1148, 1581, 1651, 1842, 1930
BmsI GCATC 7 cut(s) 118, 707, 823, 850, 1228, 1403, 2228
BpiI GAAGAC 2 cut(s) 381, 2029
BpmI CTGGAG 1 cut(s) 1563
Bpu10I CCTNAGC 1 cut(s) 123
BpuEI CTTGAG 2 cut(s) 710, 1544
BpuMI CCSGG 3 cut(s) 1008, 1148, 1651
BsaAI YACGTR 1 cut(s) 1510
BsaHI GRCGYC 2 cut(s) 459, 1832
BsaI GGTCTC 1 cut(s) 2218
BsaJI CCNNGG 5 cut(s) 984, 1037, 1086, 1147, 1929
BsaXI ACNNNNNCTCC 4 cut(s) 1499, 1529, 2083, 2113
Bse1I ACTGG 2 cut(s) 345, 538
Bse3DI GCAATG 4 cut(s) 15, 309, 586, 864
BseBI CCWGG 3 cut(s) 1581, 1842, 1930
BseDI CCNNGG 5 cut(s) 984, 1037, 1086, 1147, 1929
BseGI GGATG 3 cut(s) 133, 1300, 2239
BseMI GCAATG 4 cut(s) 15, 309, 586, 864
BseMII CTCAG 1 cut(s) 137
BseNI ACTGG 2 cut(s) 345, 538
BseRI GAGGAG 2 cut(s) 412, 780
BseSI GKGCMC 2 cut(s) 1515, 1535
BseYI CCCAGC 2 cut(s) 710, 1301
BsgI GTGCAG 1 cut(s) 1347
Bsh1236I CGCG 2 cut(s) 784, 1184
BshFI GGCC 8 cut(s) 508, 989, 1287, 1313, 1533, 1682, 1812, 2191
BshNI GGYRCC 3 cut(s) 178, 772, 1831
BsiHKAI GWGCWC 2 cut(s) 619, 726
BsiHKCI CYCGRG 1 cut(s) 2153
BsiSI CCGG 4 cut(s) 1007, 1147, 1332, 1650
BslFI GGGAC 6 cut(s) 528, 1134, 1666, 1946, 2121, 2244
BsmAI GTCTC 4 cut(s) 663, 666, 1173, 2218
BsmBI CGTCTC 1 cut(s) 1173
BsmFI GGGAC 6 cut(s) 528, 1134, 1666, 1946, 2121, 2244
BsmI GAATGC 1 cut(s) 1774
BsnI GGCC 8 cut(s) 508, 989, 1287, 1313, 1533, 1682, 1812, 2191
Bso31I GGTCTC 1 cut(s) 2218
BsoBI CYCGRG 1 cut(s) 2153
Bsp120I GGGCCC 1 cut(s) 1531
Bsp1286I GDGCHC 4 cut(s) 619, 726, 1515, 1535
Bsp1407I TGTACA 1 cut(s) 795
Bsp143I GATC 7 cut(s) 193, 256, 1257, 1542, 1855, 2014, 2063
Bsp19I CCATGG 2 cut(s) 984, 1037
BspACI CCGC 6 cut(s) 159, 696, 849, 990, 1614, 1724
BspANI GGCC 8 cut(s) 508, 989, 1287, 1313, 1533, 1682, 1812, 2191
BspCNI CTCAG 1 cut(s) 136
BspFNI CGCG 2 cut(s) 784, 1184
BspMI ACCTGC 1 cut(s) 1190
BspPI GGATC 3 cut(s) 2009, 2022, 2071
BspQI GCTCTTC 1 cut(s) 57
BspT107I GGYRCC 3 cut(s) 178, 772, 1831
BspTNI GGTCTC 1 cut(s) 2218
BsrDI GCAATG 4 cut(s) 15, 309, 586, 864
BsrGI TGTACA 1 cut(s) 795
BsrI ACTGG 2 cut(s) 345, 538
BssECI CCNNGG 5 cut(s) 984, 1037, 1086, 1147, 1929
BssMI GATC 7 cut(s) 193, 256, 1257, 1542, 1855, 2014, 2063
BssNI GRCGYC 2 cut(s) 459, 1832
BssT1I CCWWGG 3 cut(s) 984, 1037, 1086
Bst2UI CCWGG 3 cut(s) 1581, 1842, 1930
Bst6I CTCTTC 2 cut(s) 57, 1476
BstACI GRCGYC 2 cut(s) 459, 1832
BstAUI TGTACA 1 cut(s) 795
BstBAI YACGTR 1 cut(s) 1510
BstC8I GCNNGC 6 cut(s) 164, 733, 1392, 1666, 1810, 1920
BstDEI CTNAG 2 cut(s) 123, 1243
BstDSI CCRYGG 2 cut(s) 984, 1037
BstF5I GGATG 3 cut(s) 133, 1300, 2239
BstFNI CGCG 2 cut(s) 784, 1184
BstH2I RGCGCY 1 cut(s) 1835
BstHHI GCGC 4 cut(s) 784, 1013, 1186, 1834
BstKTI GATC 7 cut(s) 196, 259, 1260, 1545, 1858, 2017, 2066
BstMAI GTCTC 4 cut(s) 663, 666, 1173, 2218
BstMBI GATC 7 cut(s) 193, 256, 1257, 1542, 1855, 2014, 2063
BstNI CCWGG 3 cut(s) 1581, 1842, 1930
BstNSI RCATGY 2 cut(s) 807, 1668
BstSCI CCNGG 6 cut(s) 1006, 1146, 1579, 1649, 1840, 1928
BstSLI GKGCMC 2 cut(s) 1515, 1535
BstUI CGCG 2 cut(s) 784, 1184
BstV2I GAAGAC 2 cut(s) 381, 2029
BstX2I RGATCY 2 cut(s) 256, 2014
BstYI RGATCY 2 cut(s) 256, 2014
BsuI GTATCC 1 cut(s) 1892
BsuRI GGCC 8 cut(s) 508, 989, 1287, 1313, 1533, 1682, 1812, 2191
BtgI CCRYGG 2 cut(s) 984, 1037
BtsCI GGATG 3 cut(s) 133, 1300, 2239
BtsI GCAGTG 1 cut(s) 823
BtsIMutI CAGTG 4 cut(s) 502, 726, 823, 1909
BveI ACCTGC 1 cut(s) 1190
Cac8I GCNNGC 6 cut(s) 164, 733, 1392, 1666, 1810, 1920
CaiI CAGNNNCTG 3 cut(s) 710, 1205, 1727
CfoI GCGC 4 cut(s) 784, 1013, 1186, 1834
Cfr13I GGNCC 7 cut(s) 542, 1004, 1286, 1311, 1531, 1532, 2189
CseI GACGC 4 cut(s) 391, 448, 1190, 1796
Csp6I GTAC 3 cut(s) 179, 796, 1232
CviQI GTAC 3 cut(s) 179, 796, 1232
DdeI CTNAG 2 cut(s) 123, 1243
DinI GGCGCC 1 cut(s) 1833
DpnI GATC 7 cut(s) 195, 258, 1259, 1544, 1857, 2016, 2065
DpnII GATC 7 cut(s) 193, 256, 1257, 1542, 1855, 2014, 2063
EaeI YGGCCR 3 cut(s) 506, 987, 1680
Eam1104I CTCTTC 2 cut(s) 57, 1476
EarI CTCTTC 2 cut(s) 57, 1476
Eco130I CCWWGG 3 cut(s) 984, 1037, 1086
Eco24I GRGCYC 1 cut(s) 1535
Eco31I GGTCTC 1 cut(s) 2218
Eco32I GATATC 1 cut(s) 475
Eco47I GGWCC 2 cut(s) 542, 1004
Eco88I CYCGRG 1 cut(s) 2153
EcoO109I RGGNCCY 1 cut(s) 1531
EcoRII CCWGG 3 cut(s) 1579, 1840, 1928
EcoRV GATATC 1 cut(s) 475
EcoT14I CCWWGG 3 cut(s) 984, 1037, 1086
EcoT38I GRGCYC 1 cut(s) 1535
EgeI GGCGCC 1 cut(s) 1833
EheI GGCGCC 1 cut(s) 1833
ErhI CCWWGG 3 cut(s) 984, 1037, 1086
Esp3I CGTCTC 1 cut(s) 1173
FalI AAGNNNNNCTT 4 cut(s) 246, 278, 719, 751
FaqI GGGAC 6 cut(s) 528, 1134, 1666, 1946, 2121, 2244
FbaI TGATCA 1 cut(s) 1257
FblI GTMKAC 2 cut(s) 879, 1359
FokI GGATG 3 cut(s) 140, 1307, 2246
FriOI GRGCYC 1 cut(s) 1535
FspBI CTAG 7 cut(s) 17, 21, 845, 1221, 1779, 1827, 1941
GlaI GCGC 4 cut(s) 783, 1012, 1185, 1833
GsaI CCCAGC 2 cut(s) 714, 1305
GsuI CTGGAG 1 cut(s) 1563
HaeII RGCGCY 1 cut(s) 1835
HaeIII GGCC 8 cut(s) 508, 989, 1287, 1313, 1533, 1682, 1812, 2191
HapII CCGG 4 cut(s) 1007, 1147, 1332, 1650
HgaI GACGC 4 cut(s) 391, 448, 1190, 1796
HhaI GCGC 4 cut(s) 784, 1013, 1186, 1834
Hin1I GRCGYC 2 cut(s) 459, 1832
Hin6I GCGC 4 cut(s) 782, 1011, 1184, 1832
HinP1I GCGC 4 cut(s) 782, 1011, 1184, 1832
HincII GTYRAC 2 cut(s) 226, 1360
HindII GTYRAC 2 cut(s) 226, 1360
HindIII AAGCTT 1 cut(s) 733
HinfI GANTC 7 cut(s) 512, 659, 671, 932, 2109, 2125, 2257
HpaII CCGG 4 cut(s) 1007, 1147, 1332, 1650
HphI GGTGA 2 cut(s) 1075, 1700
Hpy166II GTNNAC 8 cut(s) 226, 796, 880, 1232, 1360, 1892, 1910, 2145
Hpy188I TCNGA 5 cut(s) 517, 614, 1344, 1542, 2019
Hpy188III TCNNGA 5 cut(s) 125, 440, 753, 1859, 2254
Hpy8I GTNNAC 8 cut(s) 226, 796, 880, 1232, 1360, 1892, 1910, 2145
Hpy99I CGWCG 2 cut(s) 902, 1364
HpyAV CCTTC 4 cut(s) 77, 995, 1833, 2001
HpyCH4IV ACGT 3 cut(s) 219, 1509, 1695
HpyF3I CTNAG 2 cut(s) 123, 1243
HpySE526I ACGT 3 cut(s) 219, 1509, 1695
Hsp92I GRCGYC 2 cut(s) 459, 1832
HspAI GCGC 4 cut(s) 782, 1011, 1184, 1832
KasI GGCGCC 1 cut(s) 1831
KpnI GGTACC 1 cut(s) 182
Ksp22I TGATCA 1 cut(s) 1257
Kzo9I GATC 7 cut(s) 193, 256, 1257, 1542, 1855, 2014, 2063
LguI GCTCTTC 1 cut(s) 57
LmnI GCTCC 6 cut(s) 399, 614, 1507, 1582, 1805, 2104
LweI GCATC 7 cut(s) 118, 707, 823, 850, 1228, 1403, 2228
MaeI CTAG 7 cut(s) 17, 21, 845, 1221, 1779, 1827, 1941
MaeII ACGT 3 cut(s) 219, 1509, 1695
MaeIII GTNAC 7 cut(s) 106, 596, 827, 1021, 1100, 2050, 2176
MalI GATC 7 cut(s) 195, 258, 1259, 1544, 1857, 2016, 2065
MboI GATC 7 cut(s) 193, 256, 1257, 1542, 1855, 2014, 2063
MfeI CAATTG 2 cut(s) 942, 1094
MflI RGATCY 2 cut(s) 256, 2014
MhlI GDGCHC 4 cut(s) 619, 726, 1515, 1535
MlsI TGGCCA 1 cut(s) 508
MluNI TGGCCA 1 cut(s) 508
Mly113I GGCGCC 1 cut(s) 1832
MlyI GAGTC 5 cut(s) 521, 665, 668, 2103, 2266
MmeI TCCRAC 3 cut(s) 495, 1389, 2041
Mox20I TGGCCA 1 cut(s) 508
MscI TGGCCA 1 cut(s) 508
MseI TTAA 5 cut(s) 450, 738, 1166, 1962, 2284
Msp20I TGGCCA 1 cut(s) 508
MspA1I CMGCKG 5 cut(s) 119, 710, 992, 1688, 1724
MspI CCGG 4 cut(s) 1007, 1147, 1332, 1650
MspR9I CCNGG 6 cut(s) 1008, 1148, 1581, 1651, 1842, 1930
MunI CAATTG 2 cut(s) 942, 1094
Mva1269I GAATGC 1 cut(s) 1774
MvaI CCWGG 3 cut(s) 1581, 1842, 1930
MvnI CGCG 2 cut(s) 784, 1184
NarI GGCGCC 1 cut(s) 1832
NciI CCSGG 3 cut(s) 1008, 1148, 1651
NcoI CCATGG 2 cut(s) 984, 1037
NdeII GATC 7 cut(s) 193, 256, 1257, 1542, 1855, 2014, 2063
NmuCI GTSAC 4 cut(s) 827, 1021, 2050, 2176
NspI RCATGY 2 cut(s) 807, 1668
PaeI GCATGC 1 cut(s) 1668
PaqCI CACCTGC 1 cut(s) 1190
PciSI GCTCTTC 1 cut(s) 57
PctI GAATGC 1 cut(s) 1774
PfeI GAWTC 2 cut(s) 932, 2125
PflMI CCANNNNNTGG 4 cut(s) 173, 558, 1616, 1841
PfoI TCCNGGA 1 cut(s) 1579
PleI GAGTC 5 cut(s) 520, 665, 667, 2103, 2265
PluTI GGCGCC 1 cut(s) 1835
PpsI GAGTC 5 cut(s) 520, 665, 667, 2103, 2265
Ppu21I YACGTR 1 cut(s) 1510
PshBI ATTAAT 1 cut(s) 1962
Psp6I CCWGG 3 cut(s) 1579, 1840, 1928
PspFI CCCAGC 2 cut(s) 710, 1301
PspGI CCWGG 3 cut(s) 1579, 1840, 1928
PspOMI GGGCCC 1 cut(s) 1531
PspPI GGNCC 7 cut(s) 542, 1004, 1286, 1311, 1531, 1532, 2189
PstNI CAGNNNCTG 3 cut(s) 710, 1205, 1727
PsuI RGATCY 2 cut(s) 256, 2014
PvuII CAGCTG 3 cut(s) 119, 710, 1688
RsaI GTAC 3 cut(s) 180, 797, 1233
RsaNI GTAC 3 cut(s) 179, 796, 1232
SalI GTCGAC 1 cut(s) 1358
SapI GCTCTTC 1 cut(s) 57
SaqAI TTAA 5 cut(s) 450, 738, 1166, 1962, 2284
Sau3AI GATC 7 cut(s) 193, 256, 1257, 1542, 1855, 2014, 2063
Sau96I GGNCC 7 cut(s) 542, 1004, 1286, 1311, 1531, 1532, 2189
SchI GAGTC 5 cut(s) 521, 665, 668, 2103, 2266
ScrFI CCNGG 6 cut(s) 1008, 1148, 1581, 1651, 1842, 1930
SduI GDGCHC 4 cut(s) 619, 726, 1515, 1535
SfaNI GCATC 7 cut(s) 118, 707, 823, 850, 1228, 1403, 2228
SfoI GGCGCC 1 cut(s) 1833
SinI GGWCC 2 cut(s) 542, 1004
SmlI CTYRAG 2 cut(s) 725, 1559
SmoI CTYRAG 2 cut(s) 725, 1559
SpeI ACTAGT 1 cut(s) 1940
SphI GCATGC 1 cut(s) 1668
SsiI CCGC 6 cut(s) 159, 696, 849, 990, 1614, 1724
SspDI GGCGCC 1 cut(s) 1831
SspI AATATT 2 cut(s) 623, 910
SspMI CTAG 7 cut(s) 17, 21, 845, 1221, 1779, 1827, 1941
StyD4I CCNGG 6 cut(s) 1006, 1146, 1579, 1649, 1840, 1928
StyI CCWWGG 3 cut(s) 984, 1037, 1086
TaiI ACGT 3 cut(s) 222, 1512, 1698
TaqI TCGA 5 cut(s) 55, 102, 395, 1359, 1526
TatI WGTACW 1 cut(s) 795
TauI GCSGC 4 cut(s) 162, 851, 992, 1727
TfiI GAWTC 2 cut(s) 932, 2125
Tru1I TTAA 5 cut(s) 450, 738, 1166, 1962, 2284
Tru9I TTAA 5 cut(s) 450, 738, 1166, 1962, 2284
TscAI CASTG 4 cut(s) 502, 726, 823, 1909
TseFI GTSAC 4 cut(s) 827, 1021, 2050, 2176
Tsp45I GTSAC 4 cut(s) 827, 1021, 2050, 2176
TspGWI ACGGA 3 cut(s) 370, 886, 1818
TspRI CASTG 4 cut(s) 502, 726, 823, 1909
Van91I CCANNNNNTGG 4 cut(s) 173, 558, 1616, 1841
VpaK11BI GGWCC 2 cut(s) 542, 1004
VspI ATTAAT 1 cut(s) 1962
XapI RAATTY 5 cut(s) 501, 1154, 1441, 1793, 2021
XceI RCATGY 2 cut(s) 807, 1668
XcmI CCANNNNNNNNNTGG 2 cut(s) 555, 1093
XmiI GTMKAC 2 cut(s) 879, 1359
XspI CTAG 7 cut(s) 17, 21, 845, 1221, 1779, 1827, 1941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.