Rh1CG071600

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0000390
Physical Location & Seq
Reverse (-)
14795862 .. 14800269
4408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG071600.1

Sequence Viewer

Length: 2037 bp
ATGGCGCCTCTTCGATGGTTTCTCCTTCTCAGCCTCATTTGCAGTATTCTACTGCTTGTCAATGTTACTCACTCAACTGCTCAGGATACCCGAAAGGCTTATGTTGTGTATATGGGCGACAAGCCAAAGAACGGGGTACCCATATTACCTGATCTTCATGTGAATATGCTACAGGACGTAGTTGACAGCAGCAACATTGATATTGCACATGAAGCTCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATGACCGGAATGGATGGTGTAGTGTCTATCTTCCCAAGCAAACAAAGGAGCGTGAAAACAACAAATAAAATCATTGGAGCACAATATTACCGTGCTTCTCGATCCTTCGAAGCAGATGTTGAGTCTCCAAGAGACTCAAATGGTCATGGAACTCATACTGCATCAACGGCAGCTGGGAACACAGTGACCAAGGCAAGCCTAAATGGTTTAGGGTTGGGAAAAGCAAGAGGAGGGGTGCCATCAGCACGTATTGCTGTGTACAAAGTATGCTGGAGCAGCACGTCTGCTTGTGAAGATGCTGACGTTTTAGCGGCATTCGATGATGCCATTGCTGACGGTGTCGACATTCTCTCTATTTCCGTCGGGGGCAGTATTCCAGTTGATTATTTAAATGATGCATTTGCAATTGGGTCATTTCATGCTACTAGAAATGGGATATTAGTTTCCATGGCCGCTGGTAACGAAGGTCCGGGAGCAAAAACTGTGACAAACTTTGCACCATGGCAACTTTCTGTGGCTGCTACCACCATAAACCGCCAATTCATCACCAAAGTTCAATTGGGTAACAGTAAAATCTATGAGGGACTTTTACCAAACATATATGACCTCCGGGGTAAATTCTATCCTTTAATTTATGGTGGAGACGCACCCAATAAAAAAGCAGGTGCTAACGGGACCCTCTCCGGGTTTTGTCTCCAAGATACCTTAGAAGAAGATTTGATCAAAGATAAAATTGTGCTTTGTGATGGCCCTGTCGATGGGGCTGGGGGTGGGGCCATATTGGGTGGTGCAGCAGGAATTGTTCTAATGGGACAAAAAGTCGCCGATGAAGTGCTTGGCCCTCTTCCCGTGCCTGCATCATACGTCGGGTTGGAAGAACATAGCGACATTTACAAATACATAAAATTAACAAGGAACGCAACTGCAACTATTTGGAAAAGTGAAGAGATTAATGATGTATTGGCTCCATATGTGCCCTCCTACTCATCAAGGGGTCCAAATCCGATCAATCCCAACATTCTCAAGCCAGATTTAGCGGCTCCAGGAACTTACATTCTAGCAGCATATCCCCCAAGTGGAGGTGGCAGTAGAGCTGGATACTATTTAGAAACTGGGACATCAATGGCGTGCCCCCATGCTACAGCCGTAGCTGCATATGTCAAATCATTTCACCCTAAATGGTCACCGGCTGCCATCCAATCGGCTCTCATCACTACCGCTAAATCTATGAGTGCCAAAACTAGCTCTGATGCTGAATTTGCATACGGAGCTGGCCTGATAAACCCTTCTAGGGCTCCATATCCTGGTTTGGTGTACGATCTTGATGAACAAGACTACATAGATTTTTTGTGTTCACAAGGATACAATGGTAAACTATTGAAAGCCATAACCAAGGACAAGACTAGTTGTTCATCAAAATCTAATAATGGAACAACCAATGACCTGAACTATCCTTCTTTTGCACTTTCCATCAAGGATCCAAAATTTGTTAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCGTTGAATTCCACATACAGAGTTAAAGTGGTGGCTCCATTGGGACTCAAAATCAATGTGAATCCAAATGTGTTATCGTTCACATCTCTCGGGCAAAAGAAATCTTTTGTTGTCACTATAAAAGGGCCGATTGAGAAATCAAATTTAGTCTCTGCGTCTTTGATGTGGGATGATGGTGTTTTCCAAGTCAGGAGCCCCATCGTTGTCTATGTTGCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

678

Amino Acids

72.1

Weight (kDa)

8.19

Isoelectric Point (pI)

34.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 113 3.8e-13 Peptidase inhibitor I9
Peptidase_S8 PF00082 121 - 519 5.4e-41 Subtilase family
fn3_6 PF17766 577 - 674 3.3e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 941
Acc36I ACCTGC 1 cut(s) 941
Acc65I GGTACC 1 cut(s) 136
AccB1I GGYRCC 3 cut(s) 4, 136, 523
AccB7I CCANNNNNTGG 1 cut(s) 1592
AccI GTMKAC 1 cut(s) 630
AciI CCGC 5 cut(s) 599, 741, 823, 1325, 1506
AclWI GGATC 4 cut(s) 384, 1760, 1773, 1822
AcoI YGGCCR 1 cut(s) 738
AcsI RAATTY 5 cut(s) 905, 1544, 1772, 1822, 1957
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 138, 548, 1604
AfiI CCNNNNNNNGG 7 cut(s) 131, 972, 1046, 1364, 1367, 1579, 1592
AgsI TTSAA 4 cut(s) 247, 845, 1669, 1822
AhdI GACNNNNNGTC 1 cut(s) 1106
AhlI ACTAGT 1 cut(s) 1691
AjiI CACGTC 1 cut(s) 570
AjnI CCWGG 2 cut(s) 1330, 1591
AluBI AGCT 7 cut(s) 215, 231, 461, 1382, 1439, 1533, 1559
AluI AGCT 7 cut(s) 215, 231, 461, 1382, 1439, 1533, 1559
Alw21I GWGCWC 1 cut(s) 370
Alw26I GTCTC 5 cut(s) 414, 417, 924, 986, 1969
AlwI GGATC 4 cut(s) 384, 1760, 1773, 1822
Ama87I CYCGRG 1 cut(s) 1904
AoxI GGCC 6 cut(s) 738, 1036, 1062, 1126, 1561, 1940
ApeKI GCWGC 9 cut(s) 189, 257, 458, 564, 806, 1079, 1349, 1439, 1478
ApoI RAATTY 5 cut(s) 905, 1544, 1772, 1822, 1957
AseI ATTAAT 1 cut(s) 1239
Asp718I GGTACC 1 cut(s) 136
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 7 cut(s) 755, 963, 1037, 1062, 1127, 1283, 1940
AsuC2I CCSGG 3 cut(s) 759, 899, 973
AsuHPI GGTGA 3 cut(s) 826, 1451, 1464
AsuII TTCGAA 1 cut(s) 396
AvaI CYCGRG 1 cut(s) 1904
AvaII GGWCC 3 cut(s) 755, 963, 1283
BaeGI GKGCMC 2 cut(s) 1266, 1421
BaeI ACNNNNGTAYC 2 cut(s) 1642, 1675
BamHI GGATCC 1 cut(s) 1765
BanI GGYRCC 3 cut(s) 4, 136, 523
BanII GRGCYC 2 cut(s) 1585, 2012
BbsI GAAGAC 1 cut(s) 1780
Bbv12I GWGCWC 1 cut(s) 370
BbvI GCAGC 9 cut(s) 201, 244, 470, 576, 793, 1091, 1361, 1426, 1465
BccI CCATC 9 cut(s) 9, 296, 535, 1028, 1040, 1490, 1766, 1982, 2021
BceAI ACGGC 2 cut(s) 471, 1418
BciT130I CCWGG 2 cut(s) 1332, 1593
BciVI GTATCC 3 cut(s) 79, 1379, 1643
BclI TGATCA 1 cut(s) 1008
BcnI CCSGG 3 cut(s) 759, 899, 973
BcoDI GTCTC 5 cut(s) 414, 417, 924, 986, 1969
BcuI ACTAGT 1 cut(s) 1691
BfaI CTAG 5 cut(s) 714, 1346, 1530, 1578, 1692
BfmI CTRYAG 2 cut(s) 170, 1428
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 941
BfuI GTATCC 3 cut(s) 79, 1379, 1643
Bme1390I CCNGG 5 cut(s) 759, 899, 973, 1332, 1593
Bme18I GGWCC 3 cut(s) 755, 963, 1283
BmeRI GACNNNNNGTC 1 cut(s) 1106
BmeT110I CYCGRG 1 cut(s) 1904
BmgBI CACGTC 1 cut(s) 570
BmgT120I GGNCC 7 cut(s) 755, 963, 1037, 1062, 1127, 1283, 1940
BmrFI CCNGG 5 cut(s) 759, 899, 973, 1332, 1593
BmrI ACTGGG 1 cut(s) 1410
BmsI GCATC 6 cut(s) 458, 574, 601, 673, 1154, 1528
BmuI ACTGGG 1 cut(s) 1410
BpiI GAAGAC 1 cut(s) 1780
BpmI CTGGAG 2 cut(s) 580, 1314
Bpu10I CCTNAGC 1 cut(s) 81
Bpu14I TTCGAA 1 cut(s) 396
BpuEI CTTGAG 1 cut(s) 1295
BpuMI CCSGG 3 cut(s) 759, 899, 973
BsaAI YACGTR 1 cut(s) 536
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 5 cut(s) 477, 735, 788, 898, 1680
BsaWI WCCGGW 1 cut(s) 293
Bsc4I CCNNNNNNNGG 7 cut(s) 131, 972, 1046, 1364, 1367, 1579, 1592
Bse118I RCCGGY 1 cut(s) 1474
Bse1I ACTGG 2 cut(s) 665, 1405
Bse3DI GCAATG 2 cut(s) 267, 615
BseBI CCWGG 2 cut(s) 1332, 1593
BseDI CCNNGG 5 cut(s) 477, 735, 788, 898, 1680
BseGI GGATG 3 cut(s) 307, 1482, 1990
BseLI CCNNNNNNNGG 7 cut(s) 131, 972, 1046, 1364, 1367, 1579, 1592
BseMI GCAATG 2 cut(s) 267, 615
BseMII CTCAG 2 cut(s) 43, 95
BseNI ACTGG 2 cut(s) 665, 1405
BseRI GAGGAG 1 cut(s) 531
BseSI GKGCMC 2 cut(s) 1266, 1421
BseXI GCAGC 9 cut(s) 201, 244, 470, 576, 793, 1091, 1361, 1426, 1465
BseYI CCCAGC 2 cut(s) 461, 1052
BsgI GTGCAG 1 cut(s) 1098
BshFI GGCC 6 cut(s) 740, 1038, 1064, 1128, 1563, 1942
BshNI GGYRCC 3 cut(s) 4, 136, 523
BsiHKAI GWGCWC 1 cut(s) 370
BsiHKCI CYCGRG 1 cut(s) 1904
BsiSI CCGG 5 cut(s) 294, 758, 898, 972, 1475
BslFI GGGAC 5 cut(s) 885, 976, 1113, 1417, 1872
BslI CCNNNNNNNGG 7 cut(s) 131, 972, 1046, 1364, 1367, 1579, 1592
BsmAI GTCTC 5 cut(s) 414, 417, 924, 986, 1969
BsmBI CGTCTC 1 cut(s) 924
BsmFI GGGAC 5 cut(s) 885, 976, 1113, 1417, 1872
BsmI GAATGC 1 cut(s) 602
BsnI GGCC 6 cut(s) 740, 1038, 1064, 1128, 1563, 1942
BsoBI CYCGRG 1 cut(s) 1904
Bsp119I TTCGAA 1 cut(s) 396
Bsp1286I GDGCHC 5 cut(s) 370, 1266, 1421, 1585, 2012
Bsp1407I TGTACA 1 cut(s) 546
Bsp143I GATC 7 cut(s) 151, 389, 1008, 1293, 1606, 1765, 1814
Bsp19I CCATGG 2 cut(s) 735, 788
BspACI CCGC 5 cut(s) 599, 741, 823, 1325, 1506
BspANI GGCC 6 cut(s) 740, 1038, 1064, 1128, 1563, 1942
BspCNI CTCAG 2 cut(s) 42, 94
BspMI ACCTGC 1 cut(s) 941
BspPI GGATC 4 cut(s) 384, 1760, 1773, 1822
BspT104I TTCGAA 1 cut(s) 396
BspT107I GGYRCC 3 cut(s) 4, 136, 523
BsrDI GCAATG 2 cut(s) 267, 615
BsrFI RCCGGY 1 cut(s) 1474
BsrGI TGTACA 1 cut(s) 546
BsrI ACTGG 2 cut(s) 665, 1405
BssAI RCCGGY 1 cut(s) 1474
BssECI CCNNGG 5 cut(s) 477, 735, 788, 898, 1680
BssMI GATC 7 cut(s) 151, 389, 1008, 1293, 1606, 1765, 1814
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 4 cut(s) 477, 735, 788, 1680
Bst2UI CCWGG 2 cut(s) 1332, 1593
Bst4CI ACNGT 6 cut(s) 380, 472, 626, 772, 857, 1801
Bst6I CTCTTC 3 cut(s) 15, 1137, 1227
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 539
BstAUI TGTACA 1 cut(s) 546
BstBAI YACGTR 1 cut(s) 536
BstBI TTCGAA 1 cut(s) 396
BstC8I GCNNGC 4 cut(s) 484, 1143, 1417, 1561
BstDEI CTNAG 3 cut(s) 29, 81, 994
BstDSI CCRYGG 2 cut(s) 735, 788
BstEII GGTNACC 1 cut(s) 1470
BstF5I GGATG 3 cut(s) 307, 1482, 1990
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 7 cut(s) 154, 392, 1011, 1296, 1609, 1768, 1817
BstMAI GTCTC 5 cut(s) 414, 417, 924, 986, 1969
BstMBI GATC 7 cut(s) 151, 389, 1008, 1293, 1606, 1765, 1814
BstMWI GCNNNNNNNGC 9 cut(s) 39, 212, 271, 455, 539, 564, 1439, 1547, 1556
BstNI CCWGG 2 cut(s) 1332, 1593
BstPI GGTNACC 1 cut(s) 1470
BstSCI CCNGG 5 cut(s) 757, 897, 971, 1330, 1591
BstSFI CTRYAG 2 cut(s) 170, 1428
BstSLI GKGCMC 2 cut(s) 1266, 1421
BstV1I GCAGC 9 cut(s) 201, 244, 470, 576, 793, 1091, 1361, 1426, 1465
BstV2I GAAGAC 1 cut(s) 1780
BstX2I RGATCY 1 cut(s) 1765
BstYI RGATCY 1 cut(s) 1765
BsuI GTATCC 3 cut(s) 79, 1379, 1643
BsuRI GGCC 6 cut(s) 740, 1038, 1064, 1128, 1563, 1942
BtgI CCRYGG 2 cut(s) 735, 788
BtrI CACGTC 1 cut(s) 570
BtsCI GGATG 3 cut(s) 307, 1482, 1990
BtsIMutI CAGTG 1 cut(s) 477
BveI ACCTGC 1 cut(s) 941
Cac8I GCNNGC 4 cut(s) 484, 1143, 1417, 1561
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 1474
Cfr13I GGNCC 7 cut(s) 755, 963, 1037, 1062, 1127, 1283, 1940
CseI GACGC 2 cut(s) 941, 1959
Csp6I GTAC 3 cut(s) 137, 547, 1603
CviAII CATG 7 cut(s) 158, 209, 434, 707, 736, 789, 1424
CviQI GTAC 3 cut(s) 137, 547, 1603
DdeI CTNAG 3 cut(s) 29, 81, 994
DinI GGCGCC 1 cut(s) 6
DpnI GATC 7 cut(s) 153, 391, 1010, 1295, 1608, 1767, 1816
DpnII GATC 7 cut(s) 151, 389, 1008, 1293, 1606, 1765, 1814
DraI TTTAAA 1 cut(s) 678
DriI GACNNNNNGTC 1 cut(s) 1106
EaeI YGGCCR 1 cut(s) 738
Eam1104I CTCTTC 3 cut(s) 15, 1137, 1227
Eam1105I GACNNNNNGTC 1 cut(s) 1106
EarI CTCTTC 3 cut(s) 15, 1137, 1227
Eco130I CCWWGG 4 cut(s) 477, 735, 788, 1680
Eco24I GRGCYC 2 cut(s) 1585, 2012
Eco47I GGWCC 3 cut(s) 755, 963, 1283
Eco88I CYCGRG 1 cut(s) 1904
Eco91I GGTNACC 1 cut(s) 1470
EcoO109I RGGNCCY 1 cut(s) 963
EcoO65I GGTNACC 1 cut(s) 1470
EcoRI GAATTC 1 cut(s) 1822
EcoRII CCWGG 2 cut(s) 1330, 1591
EcoT14I CCWWGG 4 cut(s) 477, 735, 788, 1680
EcoT22I ATGCAT 1 cut(s) 688
EcoT38I GRGCYC 2 cut(s) 1585, 2012
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 4 cut(s) 477, 735, 788, 1680
Esp3I CGTCTC 1 cut(s) 924
FaeI CATG 7 cut(s) 161, 212, 437, 710, 739, 792, 1427
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FaqI GGGAC 5 cut(s) 885, 976, 1113, 1417, 1872
FatI CATG 7 cut(s) 157, 208, 433, 706, 735, 788, 1423
FauNDI CATATG 2 cut(s) 1258, 1444
FbaI TGATCA 1 cut(s) 1008
FblI GTMKAC 1 cut(s) 630
FokI GGATG 3 cut(s) 314, 1469, 1997
FriOI GRGCYC 2 cut(s) 1585, 2012
FspBI CTAG 5 cut(s) 714, 1346, 1530, 1578, 1692
GlaI GCGC 1 cut(s) 6
GsaI CCCAGC 2 cut(s) 465, 1056
GsuI CTGGAG 2 cut(s) 580, 1314
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 6 cut(s) 740, 1038, 1064, 1128, 1563, 1942
HapII CCGG 5 cut(s) 294, 758, 898, 972, 1475
HgaI GACGC 2 cut(s) 941, 1959
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 7 cut(s) 161, 212, 437, 710, 739, 792, 1427
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 2 cut(s) 184, 631
HindII GTYRAC 2 cut(s) 184, 631
HinfI GANTC 4 cut(s) 410, 422, 1860, 1876
HpaII CCGG 5 cut(s) 294, 758, 898, 972, 1475
HphI GGTGA 3 cut(s) 826, 1451, 1464
Hpy166II GTNNAC 7 cut(s) 184, 547, 631, 1603, 1643, 1661, 1896
Hpy188I TCNGA 2 cut(s) 1293, 1537
Hpy188III TCNNGA 4 cut(s) 83, 387, 1610, 2005
Hpy8I GTNNAC 7 cut(s) 184, 547, 631, 1603, 1643, 1661, 1896
Hpy99I CGWCG 2 cut(s) 653, 1157
HpyAV CCTTC 5 cut(s) 35, 403, 746, 1584, 1752
HpyCH4III ACNGT 6 cut(s) 380, 472, 626, 772, 857, 1801
HpyCH4IV ACGT 5 cut(s) 177, 535, 569, 591, 1152
HpyF10VI GCNNNNNNNGC 9 cut(s) 39, 212, 271, 455, 539, 564, 1439, 1547, 1556
HpyF3I CTNAG 3 cut(s) 29, 81, 994
HpySE526I ACGT 5 cut(s) 177, 535, 569, 591, 1152
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 7 cut(s) 161, 212, 437, 710, 739, 792, 1427
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
KflI GGGWCCC 1 cut(s) 963
KpnI GGTACC 1 cut(s) 140
Ksp22I TGATCA 1 cut(s) 1008
Kzo9I GATC 7 cut(s) 151, 389, 1008, 1293, 1606, 1765, 1814
Lsp1109I GCAGC 9 cut(s) 201, 244, 470, 576, 793, 1091, 1361, 1426, 1465
LweI GCATC 6 cut(s) 458, 574, 601, 673, 1154, 1528
MaeI CTAG 5 cut(s) 714, 1346, 1530, 1578, 1692
MaeII ACGT 5 cut(s) 177, 535, 569, 591, 1152
MaeIII GTNAC 8 cut(s) 64, 472, 746, 772, 851, 1470, 1801, 1927
MalI GATC 7 cut(s) 153, 391, 1010, 1295, 1608, 1767, 1816
MboI GATC 7 cut(s) 151, 389, 1008, 1293, 1606, 1765, 1814
MfeI CAATTG 2 cut(s) 693, 845
MflI RGATCY 1 cut(s) 1765
MhlI GDGCHC 5 cut(s) 370, 1266, 1421, 1585, 2012
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 3 cut(s) 416, 419, 1854
MmeI TCCRAC 2 cut(s) 1140, 1792
Mph1103I ATGCAT 1 cut(s) 688
MseI TTAA 7 cut(s) 677, 917, 1196, 1239, 1779, 1839, 2035
MspA1I CMGCKG 2 cut(s) 461, 743
MspI CCGG 5 cut(s) 294, 758, 898, 972, 1475
MspR9I CCNGG 5 cut(s) 759, 899, 973, 1332, 1593
MunI CAATTG 2 cut(s) 693, 845
Mva1269I GAATGC 1 cut(s) 602
MvaI CCWGG 2 cut(s) 1332, 1593
MwoI GCNNNNNNNGC 9 cut(s) 39, 212, 271, 455, 539, 564, 1439, 1547, 1556
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 3 cut(s) 759, 899, 973
NcoI CCATGG 2 cut(s) 735, 788
NdeI CATATG 2 cut(s) 1258, 1444
NdeII GATC 7 cut(s) 151, 389, 1008, 1293, 1606, 1765, 1814
NlaIII CATG 7 cut(s) 161, 212, 437, 710, 739, 792, 1427
NmuCI GTSAC 5 cut(s) 472, 772, 1470, 1801, 1927
NsiI ATGCAT 1 cut(s) 688
NspV TTCGAA 1 cut(s) 396
PaqCI CACCTGC 1 cut(s) 941
PctI GAATGC 1 cut(s) 602
PfeI GAWTC 1 cut(s) 1876
PflFI GACNNNGTC 1 cut(s) 626
PflMI CCANNNNNTGG 1 cut(s) 1592
PfoI TCCNGGA 2 cut(s) 757, 1330
PleI GAGTC 3 cut(s) 416, 418, 1854
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 3 cut(s) 416, 418, 1854
Ppu21I YACGTR 1 cut(s) 536
PpuMI RGGWCCY 1 cut(s) 963
PshBI ATTAAT 1 cut(s) 1239
Psp5II RGGWCCY 1 cut(s) 963
Psp6I CCWGG 2 cut(s) 1330, 1591
PspEI GGTNACC 1 cut(s) 1470
PspFI CCCAGC 2 cut(s) 461, 1052
PspGI CCWGG 2 cut(s) 1330, 1591
PspPI GGNCC 7 cut(s) 755, 963, 1037, 1062, 1127, 1283, 1940
PspPPI RGGWCCY 1 cut(s) 963
PsuI RGATCY 1 cut(s) 1765
PsyI GACNNNGTC 1 cut(s) 626
PvuII CAGCTG 1 cut(s) 461
RsaI GTAC 3 cut(s) 138, 548, 1604
RsaNI GTAC 3 cut(s) 137, 547, 1603
SalI GTCGAC 1 cut(s) 629
SaqAI TTAA 7 cut(s) 677, 917, 1196, 1239, 1779, 1839, 2035
Sau3AI GATC 7 cut(s) 151, 389, 1008, 1293, 1606, 1765, 1814
Sau96I GGNCC 7 cut(s) 755, 963, 1037, 1062, 1127, 1283, 1940
SchI GAGTC 3 cut(s) 416, 419, 1854
ScrFI CCNGG 5 cut(s) 759, 899, 973, 1332, 1593
SduI GDGCHC 5 cut(s) 370, 1266, 1421, 1585, 2012
SfaNI GCATC 6 cut(s) 458, 574, 601, 673, 1154, 1528
SfcI CTRYAG 2 cut(s) 170, 1428
SfoI GGCGCC 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 396
SinI GGWCC 3 cut(s) 755, 963, 1283
SmiI ATTTAAAT 1 cut(s) 678
SmlI CTYRAG 1 cut(s) 1310
SmoI CTYRAG 1 cut(s) 1310
SpeI ACTAGT 1 cut(s) 1691
SsiI CCGC 5 cut(s) 599, 741, 823, 1325, 1506
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 374
SspMI CTAG 5 cut(s) 714, 1346, 1530, 1578, 1692
StyD4I CCNGG 5 cut(s) 757, 897, 971, 1330, 1591
StyI CCWWGG 4 cut(s) 477, 735, 788, 1680
SwaI ATTTAAAT 1 cut(s) 678
TaaI ACNGT 6 cut(s) 380, 472, 626, 772, 857, 1801
TaiI ACGT 5 cut(s) 180, 538, 572, 594, 1155
TaqI TCGA 6 cut(s) 13, 388, 396, 606, 630, 1044
TatI WGTACW 1 cut(s) 546
TauI GCSGC 3 cut(s) 602, 743, 1328
TfiI GAWTC 1 cut(s) 1876
Tru1I TTAA 7 cut(s) 677, 917, 1196, 1239, 1779, 1839, 2035
Tru9I TTAA 7 cut(s) 677, 917, 1196, 1239, 1779, 1839, 2035
TscAI CASTG 1 cut(s) 477
TseFI GTSAC 5 cut(s) 472, 772, 1470, 1801, 1927
TseI GCWGC 9 cut(s) 189, 257, 458, 564, 806, 1079, 1349, 1439, 1478
Tsp45I GTSAC 5 cut(s) 472, 772, 1470, 1801, 1927
TspDTI ATGAA 7 cut(s) 146, 225, 695, 820, 1131, 1629, 1689
TspGWI ACGGA 2 cut(s) 637, 1569
TspRI CASTG 1 cut(s) 477
Tth111I GACNNNGTC 1 cut(s) 626
Van91I CCANNNNNTGG 1 cut(s) 1592
VpaK11BI GGWCC 3 cut(s) 755, 963, 1283
VspI ATTAAT 1 cut(s) 1239
XapI RAATTY 5 cut(s) 905, 1544, 1772, 1822, 1957
XcmI CCANNNNNNNNNTGG 1 cut(s) 844
XmiI GTMKAC 1 cut(s) 630
XspI CTAG 5 cut(s) 714, 1346, 1530, 1578, 1692
Zsp2I ATGCAT 1 cut(s) 688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.