MD07G1206700.v1.1

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000390
Physical Location & Seq
Forward (+)
28512914 .. 28517756
4843 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1206700.v1.1.491

Sequence Viewer

Length: 2235 bp
ATGGCTTTAATCCCATGGTTTCTCCTTCTCATCAGCCTCATATCTACTCTGCCAGTTGATGTCACTCTATCTGCTGATCACCAGGATAACCGGAAGGATTATATTGTGTATATGGGTGACAAGCCAAAGCCAGAGGTTTCCACCACTACCACATCAGCTCTTCATGTAAACATGCTACAAAACGTCATCGATGACAGCAATATTGCGCACGAATCTCTGCTTCTGCACAGCTACAAGAGAACCTTCAATGGCTTTGCTGCCGAGCTAACAGAGGAAGAAGCACAGAAGTTGGCTGGAATGGATGGTGTGGTGTCTGTTTTCCCTAGTGAAACTAAGAAGCTCCAAACAACACGGTCATGGGACTTCATTGGGTTTCCTAAAATAGTGAAGAGAAGCGCCATTGAAACTGATATCATTGTCGGGGTGATTGACTCTGGAATTTGGCCTGAATCTGCCAGCTTCAGTGACGCCGGGTTTGGTCCACCCCCCAAAAGGTGGAAAGGCGCATGCAAGGGCGAAGGCAATTTTACTTGCAACAATAAAATTATCGGAGCACGGTATTACCGCGGTCAACCCTACCCACAAAATAGCAGTGATATCTTGTCTCCGAGGGACACGGATGGCCATGGAACCCACACTGCATCAACAGCAGCAGGGAACTTAGTTAGCAAGGCAAGTCTGTATGGTTTAGGGTTGGGGACAGCAAGAGGAGGCGTGCCATCAGCACGCATTGCGGTGTACAAAGTTTGTTGGTTAGATGGGTGCCCGGATGCTGATATTCTAGCGGCATTCGATGATGCCATAGCTGACGGTGTTGACATACTCTCTGTCTCCCTTGGGGGGTTTAAACCGTTAGATTATTTCAGAAATTCCATTGACATTGGAGCTTTTCACGCTTTAAGAAAAGGAATATTCACTTCCGCGTCTGCTGGTAATGAAGGGCCAAACCTGAAAACTATTACAAACTTTGCACCATGGTATCTTGCTGTGGCTGCTAGCACCATAGATCGCCACTTTGATACCAAGGTTCAATTGGGAAACCACAAAATTTATGAGGGAATAGTAACAAACACATTTGAACTCAAGGGTAAATTCTACCCCATAGTATATGCTGCAGACGTTCCAGATACGGCAGCTGGTTACGATGGGGAAACATCCAGGTTATGCCGAATAGGCACGTTAGACAAAAAATTGGTGGAGGGTAAAATTGTTCTTTGTGATGGGCCTACTGGGTATGGGGCTATATATGCAGGCGCATTTGGTTATGTTTTGACAAGCCGAATAGCAGCAGATGTAATCGATCCTGTTCCAGTACCAGCAGCTTCCGTTTGGTTCCACGTTGGTAACGAAATTGCCCATTACTTAAACTCAACCAGGAACCCAACAGCAACAATTTGGAAAAGTACTGAGGGCATGGATGCACTGGCCCCATACGTACCTTCATTCTCATCAAGGGGTCCAAATCCAAACACTCCTAACATTCTCAAGCCAGATATCGCTTCTCCTGGAGTTTCCATTCTAGCAGCATGGCCTCCAATTGCCCCAGTTTCAGGTTTTGAAGGTGATGACAGGGTAACTGCATACAATATATTATCGGGGACATCAATGGCATGCCCACATGCTGCGGGCGTGGCTGCATACGTCAAATCATTTCACCCCAATTGGTCACCTGCTGCTATCCTGTCAGCTCTTACGACTACTGCCAAACCTATGCGTGCAAATCTTAACCCTGAAGCCGAATTCGCATACGGTGCTGGCCTAATAAATCCTAGTAGGGCTCCGTATCCTGGTTTGGTATACGACGCTGCTGAAATCGATTACGTAAATTTTTTGTGTGCACATGGCTATAGTACCAGATTATTGAAAGCTCTTACCGGGAATAGCCATAGCTGCTCATCATCACAATCTAGTCATGGAACACTCAGTGATCATCTAAACTATCCTTCTGTTGCACTTTCCACCTCGAACCCTAAATCCGTCAATGGCATTTTCAATAGGACCGTCACAAATGTTGGATCACCGAAGTCCACATATAAAGCTAAAGTGAGTGCACCACCAGGACTTGAAATCAAAGTTAATCCAAGCGTTCTAAAGTTCACATCTCTCGGGCAAAAGCTATCGTTTCAAGTCACGGTGAAAGGGTTGATTGAAAAAACCATAGTCTCCGGTTCTCTAGTGTGGGATGATGGTAAATTCCAAGTGAGGAGCCCCATTGTTGTGTATTTTGTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

745

Amino Acids

79.65

Weight (kDa)

6.99

Isoelectric Point (pI)

30.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 34 - 114 9.4e-18 Peptidase inhibitor I9
MORF_dom PF21864 34 - 112 2.7e-06 Multiple organellar RNA editing factor 1, MORF domain
Peptidase_S8 PF00082 137 - 586 3.3e-40 Subtilase family
fn3_6 PF17766 645 - 740 6.3e-29 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1678
Acc16I TGCGCA 1 cut(s) 207
Acc36I ACCTGC 1 cut(s) 1678
AccB1I GGYRCC 1 cut(s) 762
AccB7I CCANNNNNTGG 1 cut(s) 495
AccI GTMKAC 1 cut(s) 1797
AccII CGCG 2 cut(s) 567, 923
AciI CCGC 6 cut(s) 565, 567, 734, 785, 921, 1625
AclWI GGATC 2 cut(s) 1295, 2023
AcoI YGGCCR 1 cut(s) 622
AcsI RAATTY 7 cut(s) 438, 868, 1047, 1091, 1739, 1825, 2192
AcuI CTGAAG 2 cut(s) 445, 1752
AcyI GRCGYC 1 cut(s) 468
AdeI CACNNNGTG 1 cut(s) 1925
AfaI GTAC 5 cut(s) 740, 1314, 1405, 1437, 1852
AfiI CCNNNNNNNGG 5 cut(s) 492, 495, 840, 1550, 1787
AjnI CCWGG 6 cut(s) 81, 1157, 1373, 1504, 1786, 2056
AloI GAACNNNNNNTCC 2 cut(s) 1958, 1990
Alw21I GWGCWC 3 cut(s) 556, 1840, 2053
Alw26I GTCTC 3 cut(s) 609, 835, 2167
Alw44I GTGCAC 2 cut(s) 1836, 2049
AlwI GGATC 2 cut(s) 1295, 2023
Ama87I CYCGRG 1 cut(s) 2105
AoxI GGCC 7 cut(s) 443, 622, 941, 1223, 1425, 1529, 1756
ApaLI GTGCAC 2 cut(s) 1836, 2049
ApoI RAATTY 7 cut(s) 438, 868, 1047, 1091, 1739, 1825, 2192
ArsI GACNNNNNNTTYG 2 cut(s) 458, 490
AspLEI GCGC 4 cut(s) 208, 398, 506, 1256
AspS9I GGNCC 6 cut(s) 479, 941, 1223, 1426, 1457, 1998
AsuC2I CCSGG 3 cut(s) 472, 767, 1876
AsuHPI GGTGA 8 cut(s) 71, 128, 436, 1574, 1646, 1659, 2010, 2146
AsuNHI GCTAGC 1 cut(s) 995
AvaI CYCGRG 1 cut(s) 2105
AvaII GGWCC 3 cut(s) 479, 1457, 1998
BaeGI GKGCMC 3 cut(s) 767, 1840, 2053
BaeI ACNNNNGTAYC 4 cut(s) 962, 995, 1011, 1044
BalI TGGCCA 1 cut(s) 624
BanI GGYRCC 1 cut(s) 762
BanII GRGCYC 2 cut(s) 1780, 2210
Bbv12I GWGCWC 3 cut(s) 556, 1840, 2053
BccI CCATC 7 cut(s) 296, 614, 727, 752, 1139, 1214, 2180
BceAI ACGGC 1 cut(s) 1146
BciT130I CCWGG 6 cut(s) 83, 1159, 1375, 1506, 1788, 2058
BciVI GTATCC 1 cut(s) 1794
BclI TGATCA 2 cut(s) 76, 1927
BcnI CCSGG 3 cut(s) 472, 767, 1876
BcoDI GTCTC 3 cut(s) 609, 835, 2167
BfaI CTAG 7 cut(s) 324, 782, 996, 1520, 1770, 1908, 2174
BfmI CTRYAG 2 cut(s) 1113, 1846
BfoI RGCGCY 1 cut(s) 399
BfuAI ACCTGC 1 cut(s) 1678
BfuI GTATCC 1 cut(s) 1794
BglI GCCNNNNNGGC 1 cut(s) 1173
BmcAI AGTACT 1 cut(s) 1405
Bme1390I CCNGG 9 cut(s) 83, 472, 767, 1159, 1375, 1506, 1788, 1876, 2058
Bme18I GGWCC 3 cut(s) 479, 1457, 1998
BmeT110I CYCGRG 1 cut(s) 2105
BmgT120I GGNCC 6 cut(s) 479, 941, 1223, 1426, 1457, 1998
BmiI GGNNCC 8 cut(s) 631, 764, 1334, 1379, 1428, 1458, 1779, 2207
BmrFI CCNGG 9 cut(s) 83, 472, 767, 1159, 1375, 1506, 1788, 1876, 2058
BmrI ACTGGG 2 cut(s) 1239, 1538
BmsI GCATC 4 cut(s) 650, 760, 787, 1408
BmtI GCTAGC 1 cut(s) 999
BmuI ACTGGG 2 cut(s) 1239, 1538
BpmI CTGGAG 1 cut(s) 1527
BpuEI CTTGAG 2 cut(s) 1067, 1469
BpuMI CCSGG 3 cut(s) 472, 767, 1876
Bsa29I ATCGAT 3 cut(s) 189, 1299, 1815
BsaAI YACGTR 2 cut(s) 1435, 1822
BsaHI GRCGYC 1 cut(s) 468
BsaJI CCNNGG 7 cut(s) 14, 565, 608, 625, 835, 974, 1023
BsaWI WCCGGW 2 cut(s) 90, 2165
Bsc4I CCNNNNNNNGG 5 cut(s) 492, 495, 840, 1550, 1787
Bse1I ACTGG 5 cut(s) 53, 1234, 1310, 1428, 1544
Bse3DI GCAATG 1 cut(s) 729
BseBI CCWGG 6 cut(s) 83, 1159, 1375, 1506, 1788, 2058
BseCI ATCGAT 3 cut(s) 189, 1299, 1815
BseDI CCNNGG 7 cut(s) 14, 565, 608, 625, 835, 974, 1023
BseGI GGATG 6 cut(s) 307, 625, 775, 1154, 1423, 2188
BseLI CCNNNNNNNGG 5 cut(s) 492, 495, 840, 1550, 1787
BseMI GCAATG 1 cut(s) 729
BseMII CTCAG 2 cut(s) 1398, 1936
BseNI ACTGG 5 cut(s) 53, 1234, 1310, 1428, 1544
BseRI GAGGAG 2 cut(s) 723, 2218
BseSI GKGCMC 3 cut(s) 767, 1840, 2053
BsgI GTGCAG 1 cut(s) 209
Bsh1236I CGCG 2 cut(s) 567, 923
BshFI GGCC 7 cut(s) 445, 624, 943, 1225, 1427, 1531, 1758
BshNI GGYRCC 1 cut(s) 762
BshVI ATCGAT 3 cut(s) 189, 1299, 1815
BsiHKAI GWGCWC 3 cut(s) 556, 1840, 2053
BsiHKCI CYCGRG 1 cut(s) 2105
BsiSI CCGG 5 cut(s) 91, 471, 767, 1875, 2166
BslFI GGGAC 4 cut(s) 374, 626, 712, 1612
BslI CCNNNNNNNGG 5 cut(s) 492, 495, 840, 1550, 1787
BsmAI GTCTC 3 cut(s) 609, 835, 2167
BsmFI GGGAC 4 cut(s) 374, 626, 712, 1612
BsmI GAATGC 1 cut(s) 788
BsnI GGCC 7 cut(s) 445, 624, 943, 1225, 1427, 1531, 1758
BsoBI CYCGRG 1 cut(s) 2105
Bsp1286I GDGCHC 6 cut(s) 556, 767, 1780, 1840, 2053, 2210
Bsp1407I TGTACA 1 cut(s) 738
Bsp143I GATC 5 cut(s) 76, 1006, 1300, 1927, 2015
Bsp19I CCATGG 3 cut(s) 14, 625, 974
BspACI CCGC 6 cut(s) 565, 567, 734, 785, 921, 1625
BspANI GGCC 7 cut(s) 445, 624, 943, 1225, 1427, 1531, 1758
BspCNI CTCAG 2 cut(s) 1399, 1935
BspDI ATCGAT 3 cut(s) 189, 1299, 1815
BspFNI CGCG 2 cut(s) 567, 923
BspLI GGNNCC 8 cut(s) 631, 764, 1334, 1379, 1428, 1458, 1779, 2207
BspMAI CTGCAG 1 cut(s) 1117
BspMI ACCTGC 1 cut(s) 1678
BspOI GCTAGC 1 cut(s) 999
BspPI GGATC 2 cut(s) 1295, 2023
BspQI GCTCTTC 1 cut(s) 165
BspT107I GGYRCC 1 cut(s) 762
BsrDI GCAATG 1 cut(s) 729
BsrGI TGTACA 1 cut(s) 738
BsrI ACTGG 5 cut(s) 53, 1234, 1310, 1428, 1544
BssECI CCNNGG 7 cut(s) 14, 565, 608, 625, 835, 974, 1023
BssMI GATC 5 cut(s) 76, 1006, 1300, 1927, 2015
BssNAI GTATAC 1 cut(s) 1798
BssNI GRCGYC 1 cut(s) 468
BssT1I CCWWGG 5 cut(s) 14, 625, 835, 974, 1023
Bst1107I GTATAC 1 cut(s) 1798
Bst2UI CCWGG 6 cut(s) 83, 1159, 1375, 1506, 1788, 2058
Bst4CI ACNGT 7 cut(s) 354, 558, 812, 852, 1751, 2002, 2134
Bst6I CTCTTC 2 cut(s) 165, 383
BstACI GRCGYC 1 cut(s) 468
BstAPI GCANNNNNTGC 2 cut(s) 731, 1751
BstAUI TGTACA 1 cut(s) 738
BstBAI YACGTR 2 cut(s) 1435, 1822
BstDEI CTNAG 4 cut(s) 333, 661, 1407, 1922
BstDSI CCRYGG 4 cut(s) 14, 565, 625, 974
BstEII GGTNACC 1 cut(s) 1665
BstF5I GGATG 6 cut(s) 307, 625, 775, 1154, 1423, 2188
BstFNI CGCG 2 cut(s) 567, 923
BstH2I RGCGCY 1 cut(s) 399
BstHHI GCGC 4 cut(s) 208, 398, 506, 1256
BstKTI GATC 5 cut(s) 79, 1009, 1303, 1930, 2018
BstMAI GTCTC 3 cut(s) 609, 835, 2167
BstMBI GATC 5 cut(s) 76, 1006, 1300, 1927, 2015
BstNI CCWGG 6 cut(s) 83, 1159, 1375, 1506, 1788, 2058
BstNSI RCATGY 4 cut(s) 175, 510, 1614, 1622
BstPI GGTNACC 1 cut(s) 1665
BstSCI CCNGG 9 cut(s) 81, 470, 765, 1157, 1373, 1504, 1786, 1874, 2056
BstSFI CTRYAG 2 cut(s) 1113, 1846
BstSLI GKGCMC 3 cut(s) 767, 1840, 2053
BstSNI TACGTA 2 cut(s) 1435, 1822
BstUI CGCG 2 cut(s) 567, 923
BstZ17I GTATAC 1 cut(s) 1798
Bsu15I ATCGAT 3 cut(s) 189, 1299, 1815
BsuI GTATCC 1 cut(s) 1794
BsuRI GGCC 7 cut(s) 445, 624, 943, 1225, 1427, 1531, 1758
BsuTUI ATCGAT 3 cut(s) 189, 1299, 1815
BtgI CCRYGG 4 cut(s) 14, 565, 625, 974
BtsCI GGATG 6 cut(s) 307, 625, 775, 1154, 1423, 2188
BtsI GCAGTG 2 cut(s) 598, 636
BtsIMutI CAGTG 5 cut(s) 469, 598, 636, 1421, 1930
BveI ACCTGC 1 cut(s) 1678
CfoI GCGC 4 cut(s) 208, 398, 506, 1256
Cfr13I GGNCC 6 cut(s) 479, 941, 1223, 1426, 1457, 1998
Cfr42I CCGCGG 1 cut(s) 568
ClaI ATCGAT 3 cut(s) 189, 1299, 1815
CseI GACGC 3 cut(s) 476, 912, 1811
Csp6I GTAC 5 cut(s) 739, 1313, 1404, 1436, 1851
CviQI GTAC 5 cut(s) 739, 1313, 1404, 1436, 1851
DdeI CTNAG 4 cut(s) 333, 661, 1407, 1922
DpnI GATC 5 cut(s) 78, 1008, 1302, 1929, 2017
DpnII GATC 5 cut(s) 76, 1006, 1300, 1927, 2015
DraI TTTAAA 1 cut(s) 847
DraIII CACNNNGTG 1 cut(s) 1925
EaeI YGGCCR 1 cut(s) 622
Eam1104I CTCTTC 2 cut(s) 165, 383
EarI CTCTTC 2 cut(s) 165, 383
Eco105I TACGTA 2 cut(s) 1435, 1822
Eco130I CCWWGG 5 cut(s) 14, 625, 835, 974, 1023
Eco24I GRGCYC 2 cut(s) 1780, 2210
Eco32I GATATC 3 cut(s) 412, 598, 1495
Eco47I GGWCC 3 cut(s) 479, 1457, 1998
Eco57I CTGAAG 2 cut(s) 445, 1752
Eco88I CYCGRG 1 cut(s) 2105
Eco91I GGTNACC 1 cut(s) 1665
EcoO65I GGTNACC 1 cut(s) 1665
EcoRI GAATTC 1 cut(s) 1739
EcoRII CCWGG 6 cut(s) 81, 1157, 1373, 1504, 1786, 2056
EcoRV GATATC 3 cut(s) 412, 598, 1495
EcoT14I CCWWGG 5 cut(s) 14, 625, 835, 974, 1023
EcoT38I GRGCYC 2 cut(s) 1780, 2210
ErhI CCWWGG 5 cut(s) 14, 625, 835, 974, 1023
FalI AAGNNNNNCTT 2 cut(s) 227, 259
FaqI GGGAC 4 cut(s) 374, 626, 712, 1612
FauI CCCGC 1 cut(s) 1618
FbaI TGATCA 2 cut(s) 76, 1927
FblI GTMKAC 1 cut(s) 1797
FokI GGATG 6 cut(s) 314, 632, 782, 1141, 1430, 2195
FriOI GRGCYC 2 cut(s) 1780, 2210
FspBI CTAG 7 cut(s) 324, 782, 996, 1520, 1770, 1908, 2174
FspI TGCGCA 1 cut(s) 207
GlaI GCGC 4 cut(s) 207, 397, 505, 1255
GsuI CTGGAG 1 cut(s) 1527
HaeII RGCGCY 1 cut(s) 399
HaeIII GGCC 7 cut(s) 445, 624, 943, 1225, 1427, 1531, 1758
HapII CCGG 5 cut(s) 91, 471, 767, 1875, 2166
HgaI GACGC 3 cut(s) 476, 912, 1811
HhaI GCGC 4 cut(s) 208, 398, 506, 1256
Hin1I GRCGYC 1 cut(s) 468
Hin6I GCGC 4 cut(s) 206, 396, 504, 1254
HinP1I GCGC 4 cut(s) 206, 396, 504, 1254
HincII GTYRAC 2 cut(s) 572, 817
HindII GTYRAC 2 cut(s) 572, 817
HinfI GANTC 3 cut(s) 212, 431, 449
HpaII CCGG 5 cut(s) 91, 471, 767, 1875, 2166
HphI GGTGA 8 cut(s) 71, 128, 436, 1574, 1646, 1659, 2010, 2146
Hpy188I TCNGA 3 cut(s) 551, 609, 866
Hpy188III TCNNGA 2 cut(s) 435, 1124
Hpy99I CGWCG 1 cut(s) 1805
HpyAV CCTTC 8 cut(s) 35, 88, 253, 512, 932, 1449, 1553, 1953
HpyCH4III ACNGT 7 cut(s) 354, 558, 812, 852, 1751, 2002, 2134
HpyCH4IV ACGT 7 cut(s) 183, 1119, 1178, 1338, 1434, 1641, 1821
HpyF3I CTNAG 4 cut(s) 333, 661, 1407, 1922
HpySE526I ACGT 7 cut(s) 183, 1119, 1178, 1338, 1434, 1641, 1821
Hsp92I GRCGYC 1 cut(s) 468
HspAI GCGC 4 cut(s) 206, 396, 504, 1254
Ksp22I TGATCA 2 cut(s) 76, 1927
KspI CCGCGG 1 cut(s) 568
Kzo9I GATC 5 cut(s) 76, 1006, 1300, 1927, 2015
LguI GCTCTTC 1 cut(s) 165
LmnI GCTCC 5 cut(s) 345, 551, 884, 1783, 2205
LweI GCATC 4 cut(s) 650, 760, 787, 1408
MaeI CTAG 7 cut(s) 324, 782, 996, 1520, 1770, 1908, 2174
MaeII ACGT 7 cut(s) 183, 1119, 1178, 1338, 1434, 1641, 1821
MalI GATC 5 cut(s) 78, 1008, 1302, 1929, 2017
MboI GATC 5 cut(s) 76, 1006, 1300, 1927, 2015
MboII GAAGA 3 cut(s) 152, 287, 400
MfeI CAATTG 3 cut(s) 1031, 1536, 1660
MhlI GDGCHC 6 cut(s) 556, 767, 1780, 1840, 2053, 2210
MlsI TGGCCA 1 cut(s) 624
MluNI TGGCCA 1 cut(s) 624
MlyI GAGTC 1 cut(s) 425
MmeI TCCRAC 1 cut(s) 1993
Mox20I TGGCCA 1 cut(s) 624
MscI TGGCCA 1 cut(s) 624
MseI TTAA 6 cut(s) 8, 846, 899, 1364, 1725, 2076
MslI CAYNNNNRTG 3 cut(s) 355, 734, 2216
Msp20I TGGCCA 1 cut(s) 624
MspA1I CMGCKG 2 cut(s) 567, 1136
MspI CCGG 5 cut(s) 91, 471, 767, 1875, 2166
MspR9I CCNGG 9 cut(s) 83, 472, 767, 1159, 1375, 1506, 1788, 1876, 2058
MssI GTTTAAAC 1 cut(s) 847
MunI CAATTG 3 cut(s) 1031, 1536, 1660
Mva1269I GAATGC 1 cut(s) 788
MvaI CCWGG 6 cut(s) 83, 1159, 1375, 1506, 1788, 2058
MvnI CGCG 2 cut(s) 567, 923
NciI CCSGG 3 cut(s) 472, 767, 1876
NcoI CCATGG 3 cut(s) 14, 625, 974
NdeII GATC 5 cut(s) 76, 1006, 1300, 1927, 2015
NheI GCTAGC 1 cut(s) 995
NlaIV GGNNCC 8 cut(s) 631, 764, 1334, 1379, 1428, 1458, 1779, 2207
NmeAIII GCCGAG 1 cut(s) 286
NmuCI GTSAC 6 cut(s) 61, 116, 464, 1665, 2002, 2128
NsbI TGCGCA 1 cut(s) 207
NspI RCATGY 4 cut(s) 175, 510, 1614, 1622
PaeI GCATGC 2 cut(s) 510, 1614
PaqCI CACCTGC 1 cut(s) 1678
PciSI GCTCTTC 1 cut(s) 165
PcsI WCGNNNNNNNCGW 1 cut(s) 1344
PctI GAATGC 1 cut(s) 788
PfeI GAWTC 2 cut(s) 212, 449
PflMI CCANNNNNTGG 1 cut(s) 495
PfoI TCCNGGA 1 cut(s) 1504
PleI GAGTC 1 cut(s) 425
PmeI GTTTAAAC 1 cut(s) 847
PpsI GAGTC 1 cut(s) 425
Ppu21I YACGTR 2 cut(s) 1435, 1822
Psp6I CCWGG 6 cut(s) 81, 1157, 1373, 1504, 1786, 2056
PspEI GGTNACC 1 cut(s) 1665
PspGI CCWGG 6 cut(s) 81, 1157, 1373, 1504, 1786, 2056
PspN4I GGNNCC 8 cut(s) 631, 764, 1334, 1379, 1428, 1458, 1779, 2207
PspPI GGNCC 6 cut(s) 479, 941, 1223, 1426, 1457, 1998
PstI CTGCAG 1 cut(s) 1117
PvuII CAGCTG 1 cut(s) 1136
RsaI GTAC 5 cut(s) 740, 1314, 1405, 1437, 1852
RsaNI GTAC 5 cut(s) 739, 1313, 1404, 1436, 1851
RseI CAYNNNNRTG 3 cut(s) 355, 734, 2216
SacII CCGCGG 1 cut(s) 568
SapI GCTCTTC 1 cut(s) 165
SaqAI TTAA 6 cut(s) 8, 846, 899, 1364, 1725, 2076
Sau3AI GATC 5 cut(s) 76, 1006, 1300, 1927, 2015
Sau96I GGNCC 6 cut(s) 479, 941, 1223, 1426, 1457, 1998
ScaI AGTACT 1 cut(s) 1405
SchI GAGTC 1 cut(s) 425
ScrFI CCNGG 9 cut(s) 83, 472, 767, 1159, 1375, 1506, 1788, 1876, 2058
SduI GDGCHC 6 cut(s) 556, 767, 1780, 1840, 2053, 2210
SfaNI GCATC 4 cut(s) 650, 760, 787, 1408
SfcI CTRYAG 2 cut(s) 1113, 1846
Sfr303I CCGCGG 1 cut(s) 568
SgrBI CCGCGG 1 cut(s) 568
SinI GGWCC 3 cut(s) 479, 1457, 1998
SmiMI CAYNNNNRTG 3 cut(s) 355, 734, 2216
SmlI CTYRAG 2 cut(s) 1082, 1484
SmoI CTYRAG 2 cut(s) 1082, 1484
SnaBI TACGTA 2 cut(s) 1435, 1822
SphI GCATGC 2 cut(s) 510, 1614
SsiI CCGC 6 cut(s) 565, 567, 734, 785, 921, 1625
SspI AATATT 2 cut(s) 202, 912
SspMI CTAG 7 cut(s) 324, 782, 996, 1520, 1770, 1908, 2174
StyD4I CCNGG 9 cut(s) 81, 470, 765, 1157, 1373, 1504, 1786, 1874, 2056
StyI CCWWGG 5 cut(s) 14, 625, 835, 974, 1023
TaaI ACNGT 7 cut(s) 354, 558, 812, 852, 1751, 2002, 2134
TaiI ACGT 7 cut(s) 186, 1122, 1181, 1341, 1437, 1644, 1824
TaqI TCGA 5 cut(s) 189, 792, 1299, 1815, 1964
TatI WGTACW 2 cut(s) 738, 1403
TauI GCSGC 1 cut(s) 788
TfiI GAWTC 2 cut(s) 212, 449
Tru1I TTAA 6 cut(s) 8, 846, 899, 1364, 1725, 2076
Tru9I TTAA 6 cut(s) 8, 846, 899, 1364, 1725, 2076
TscAI CASTG 5 cut(s) 469, 598, 643, 1428, 1930
TseFI GTSAC 6 cut(s) 61, 116, 464, 1665, 2002, 2128
Tsp45I GTSAC 6 cut(s) 61, 116, 464, 1665, 2002, 2128
TspDTI ATGAA 4 cut(s) 152, 355, 951, 1431
TspGWI ACGGA 4 cut(s) 632, 1315, 1770, 1966
TspRI CASTG 5 cut(s) 469, 598, 643, 1428, 1930
Van91I CCANNNNNTGG 1 cut(s) 495
VneI GTGCAC 2 cut(s) 1836, 2049
VpaK11BI GGWCC 3 cut(s) 479, 1457, 1998
XapI RAATTY 7 cut(s) 438, 868, 1047, 1091, 1739, 1825, 2192
XceI RCATGY 4 cut(s) 175, 510, 1614, 1622
XcmI CCANNNNNNNNNTGG 1 cut(s) 1030
XmiI GTMKAC 1 cut(s) 1797
XspI CTAG 7 cut(s) 324, 782, 996, 1520, 1770, 1908, 2174
ZrmI AGTACT 1 cut(s) 1405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.