Rroxscaffold_4G00324120

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000390
Physical Location & Seq
Forward (+)
55419643 .. 55422338
2696 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00324120.1

Sequence Viewer

Length: 1764 bp
ATGGAACTTCCTTGGGTTTCCAGAAAGGCAGAAACAGTTAAGCGAAGCGCCCTTGAAAGGAATATCATCGTCGGTGTGATAGACACTGGAATTTGGCCAGAGTCGGACAGCTTTAGTGATGCTGGTTTTGGTCCTCCACCTGAGAAATGGAAAGGCAAATGTCAAGGCAATGGAAATCTTACTTATTTTGAGTCTCCAAGAGACTCAAATGGTCATGGAACGCATACTGCATCAACAGCAGCTGGGAACACAGTGAGCAAGGCAAGCCTAAATGGTTTAGGGTTGGGAACAGCAAGAGGCGGGGTGCCATCAGCACGTATTGCCGTGTACAAAGTATGCTGGAGCAGCAGGCCTGCTTGTGAAGATGCTGACATTTTAGCGGCATTCGATGATGCCATTGCTGACGGTGTCGACATTCTCTCTATTTCTATCGGGAGCGCTATTCCAGTTGATTATTTGACTGATTCATTGGCAATTGGGTCATTTCACGCTACCAGAAATGGGATATTAGTTTCCCTGGTCGCTGGTAACGAAGGTCCGGGAGCAAAAACTGTGACAAACTTTGCACCATGGCAACTTTCTGTGGCTGCTTCCACCATAAACCGCCAATTCATCACCAAGGTTCAATTGGGTAACGGTAAAATCTATGAGGGACTTTTACCAAACATATATGACCTCCGGGGTAAATTATATCCTTTAATTTATGGTGGAGACGCACCCAACAAAAAAGTAGGTGCTAAAGGGACCGACTCCAGGTTTTGTCTCATAGATACCTTAGAAGAAGATTTGATCAAAGATAAAATTGTGCTTTGTGATGGCCCTGTTGGGGATGGAGCCATATTGGGTGGTGCAGCCGGAATTGTTCTAACAGGACAAAAAGTTGCCGATGAAGTGCTTGAGCCTCTTCCCGTGCCTGCATCAAACGTCGGGAACGCAACTGCAACTATTTGGAAAAGTGAAGAGATTAATGATGTATTGGCTCCATATGTGCCCTCATACTCATCAAGGGGTCCAAATCCGATCAGTCCCAACATTCTCAAGCCAGATTTAGCGGCTCCAGGAACTTACATTCTAGCAGCATATCCCCCAAGTGGTGGTGGTAGTAGAGCTGGATATTATTTAGAAACTGGGACATCAATGGCGTGCCCCCATGCTACAGCCGTAGCTGCATACGTCAAATCATTTCACCCTAAATGGTCACCGGCTGCTATCCAATCGGCTCTCATCACTACCGCTAAATCTATGAGTGCCAAAACTAGCCCTAATGCTGAATTTGCATACGGAGCTGGCCTGATAAACCCTTCTAAGGCTCCATATCCTGGTTTGGTGTACGATCTTGATGAACAAGACTACATAGATTTTTTGTGTTCACAAGGATATAGTGGTAAACTATTGCAAGCCATAACCAAGGACAACACTAGTTGTTCATCAAAATCTAATAATGGAACAACCAATGACCTAAACTATCCTTCTATTGCACTTTCCATCAAGGATCCGAAATTTGTCAATGGGGTCTTCAATAGGACTGTTACTAATGTTGGATCGTCGAATTCCACATACAGAGTCAAAGTGGTGGCTCCTTTAGGACTCAAAATCAATGTGAATCCAAGTGTGCTATCATTCACATCTCTGGGGCAAAAGAAATCTTTTGTTGTCACTATAAACGGGCCGATTGAGAAATCAAATTTAGTCTCTGCATCTTTAGTGTGGGATGATGGTGTTTTCCAAGTCAGGAGCCCCATCGTTGTCTATGTTGCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

587

Amino Acids

61.68

Weight (kDa)

6.55

Isoelectric Point (pI)

32.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 20 - 430 1.1e-44 Subtilase family
fn3_6 PF17766 486 - 583 7.3e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 304
AccB7I CCANNNNNTGG 2 cut(s) 1094, 1319
AccI GTMKAC 1 cut(s) 411
AciI CCGC 5 cut(s) 300, 380, 604, 1052, 1233
AclWI GGATC 3 cut(s) 1487, 1500, 1549
AcoI YGGCCR 1 cut(s) 95
AcsI RAATTY 5 cut(s) 90, 1271, 1499, 1549, 1684
AfaI GTAC 2 cut(s) 329, 1331
AfeI AGCGCT 1 cut(s) 439
AfiI CCNNNNNNNGG 8 cut(s) 57, 501, 753, 826, 1091, 1094, 1306, 1319
AgsI TTSAA 3 cut(s) 56, 626, 1519
AhlI ACTAGT 1 cut(s) 1418
AjnI CCWGG 4 cut(s) 516, 752, 1057, 1318
AluBI AGCT 5 cut(s) 111, 242, 1109, 1166, 1286
AluI AGCT 5 cut(s) 111, 242, 1109, 1166, 1286
Alw26I GTCTC 5 cut(s) 195, 198, 705, 767, 1696
AlwI GGATC 3 cut(s) 1487, 1500, 1549
AlwNI CAGNNNCTG 1 cut(s) 242
Aor51HI AGCGCT 1 cut(s) 439
AoxI GGCC 5 cut(s) 95, 350, 817, 1288, 1667
ApeKI GCWGC 7 cut(s) 239, 345, 587, 851, 1076, 1166, 1205
ApoI RAATTY 5 cut(s) 90, 1271, 1499, 1549, 1684
AseI ATTAAT 1 cut(s) 966
AspLEI GCGC 2 cut(s) 50, 440
AspS9I GGNCC 6 cut(s) 131, 536, 744, 818, 1010, 1667
AsuC2I CCSGG 2 cut(s) 540, 680
AsuHPI GGTGA 3 cut(s) 607, 1178, 1191
AvaII GGWCC 4 cut(s) 131, 536, 744, 1010
BaeGI GKGCMC 2 cut(s) 993, 1148
BalI TGGCCA 1 cut(s) 97
BamHI GGATCC 1 cut(s) 1492
BanI GGYRCC 1 cut(s) 304
BanII GRGCYC 1 cut(s) 1739
BbsI GAAGAC 1 cut(s) 1507
BbvI GCAGC 7 cut(s) 251, 357, 574, 863, 1088, 1153, 1192
BccI CCATC 6 cut(s) 316, 809, 824, 1493, 1709, 1748
BceAI ACGGC 2 cut(s) 308, 1145
BciT130I CCWGG 4 cut(s) 518, 754, 1059, 1320
BclI TGATCA 1 cut(s) 789
BcnI CCSGG 2 cut(s) 540, 680
BcoDI GTCTC 5 cut(s) 195, 198, 705, 767, 1696
BcuI ACTAGT 1 cut(s) 1418
BfaI CTAG 3 cut(s) 1073, 1257, 1419
BfmI CTRYAG 1 cut(s) 1155
BfoI RGCGCY 2 cut(s) 51, 441
BisI GCNGC 9 cut(s) 240, 346, 381, 588, 852, 1053, 1077, 1167, 1206
BlsI GCNGC 9 cut(s) 241, 347, 382, 589, 853, 1054, 1078, 1168, 1207
Bme1390I CCNGG 6 cut(s) 518, 540, 680, 754, 1059, 1320
Bme18I GGWCC 4 cut(s) 131, 536, 744, 1010
BmgT120I GGNCC 6 cut(s) 131, 536, 744, 818, 1010, 1667
BmrFI CCNGG 6 cut(s) 518, 540, 680, 754, 1059, 1320
BmrI ACTGGG 1 cut(s) 1137
BmsI GCATC 6 cut(s) 109, 239, 355, 382, 926, 1706
BmuI ACTGGG 1 cut(s) 1137
BpiI GAAGAC 1 cut(s) 1507
BpmI CTGGAG 3 cut(s) 361, 736, 1041
BpuEI CTTGAG 2 cut(s) 917, 1022
BpuMI CCSGG 2 cut(s) 540, 680
BsaAI YACGTR 1 cut(s) 317
BsaJI CCNNGG 6 cut(s) 11, 516, 569, 618, 679, 1407
Bsc4I CCNNNNNNNGG 8 cut(s) 57, 501, 753, 826, 1091, 1094, 1306, 1319
Bse118I RCCGGY 1 cut(s) 1201
Bse1I ACTGG 3 cut(s) 91, 446, 1132
Bse3DI GCAATG 2 cut(s) 175, 396
BseBI CCWGG 4 cut(s) 518, 754, 1059, 1320
BseDI CCNNGG 6 cut(s) 11, 516, 569, 618, 679, 1407
BseGI GGATG 2 cut(s) 835, 1717
BseLI CCNNNNNNNGG 8 cut(s) 57, 501, 753, 826, 1091, 1094, 1306, 1319
BseMI GCAATG 2 cut(s) 175, 396
BseMII CTCAG 1 cut(s) 132
BseNI ACTGG 3 cut(s) 91, 446, 1132
BseSI GKGCMC 2 cut(s) 993, 1148
BseXI GCAGC 7 cut(s) 251, 357, 574, 863, 1088, 1153, 1192
BseYI CCCAGC 1 cut(s) 242
BsgI GTGCAG 1 cut(s) 870
BshFI GGCC 5 cut(s) 97, 352, 819, 1290, 1669
BshNI GGYRCC 1 cut(s) 304
BsiSI CCGG 4 cut(s) 539, 679, 855, 1202
BslFI GGGAC 4 cut(s) 666, 757, 1011, 1144
BslI CCNNNNNNNGG 8 cut(s) 57, 501, 753, 826, 1091, 1094, 1306, 1319
BsmAI GTCTC 5 cut(s) 195, 198, 705, 767, 1696
BsmBI CGTCTC 1 cut(s) 705
BsmFI GGGAC 4 cut(s) 666, 757, 1011, 1144
BsmI GAATGC 1 cut(s) 383
BsnI GGCC 5 cut(s) 97, 352, 819, 1290, 1669
Bsp1286I GDGCHC 3 cut(s) 993, 1148, 1739
Bsp1407I TGTACA 1 cut(s) 327
Bsp143I GATC 5 cut(s) 789, 1020, 1333, 1492, 1541
Bsp19I CCATGG 1 cut(s) 569
BspACI CCGC 5 cut(s) 300, 380, 604, 1052, 1233
BspANI GGCC 5 cut(s) 97, 352, 819, 1290, 1669
BspCNI CTCAG 1 cut(s) 133
BspPI GGATC 3 cut(s) 1487, 1500, 1549
BspT107I GGYRCC 1 cut(s) 304
BsrDI GCAATG 2 cut(s) 175, 396
BsrFI RCCGGY 1 cut(s) 1201
BsrGI TGTACA 1 cut(s) 327
BsrI ACTGG 3 cut(s) 91, 446, 1132
BssAI RCCGGY 1 cut(s) 1201
BssECI CCNNGG 6 cut(s) 11, 516, 569, 618, 679, 1407
BssMI GATC 5 cut(s) 789, 1020, 1333, 1492, 1541
BssT1I CCWWGG 4 cut(s) 11, 569, 618, 1407
Bst2UI CCWGG 4 cut(s) 518, 754, 1059, 1320
Bst4CI ACNGT 6 cut(s) 37, 253, 407, 553, 638, 1528
Bst6I CTCTTC 2 cut(s) 909, 954
BstAPI GCANNNNNTGC 1 cut(s) 320
BstAUI TGTACA 1 cut(s) 327
BstBAI YACGTR 1 cut(s) 317
BstC8I GCNNGC 7 cut(s) 265, 350, 354, 915, 1144, 1288, 1398
BstDEI CTNAG 3 cut(s) 141, 775, 1305
BstDSI CCRYGG 1 cut(s) 569
BstEII GGTNACC 1 cut(s) 1197
BstF5I GGATG 2 cut(s) 835, 1717
BstH2I RGCGCY 2 cut(s) 51, 441
BstHHI GCGC 2 cut(s) 50, 440
BstKTI GATC 5 cut(s) 792, 1023, 1336, 1495, 1544
BstMAI GTCTC 5 cut(s) 195, 198, 705, 767, 1696
BstMBI GATC 5 cut(s) 789, 1020, 1333, 1492, 1541
BstMWI GCNNNNNNNGC 7 cut(s) 236, 264, 320, 345, 1166, 1274, 1283
BstNI CCWGG 4 cut(s) 518, 754, 1059, 1320
BstPI GGTNACC 1 cut(s) 1197
BstSCI CCNGG 6 cut(s) 516, 538, 678, 752, 1057, 1318
BstSFI CTRYAG 1 cut(s) 1155
BstSLI GKGCMC 2 cut(s) 993, 1148
BstV1I GCAGC 7 cut(s) 251, 357, 574, 863, 1088, 1153, 1192
BstV2I GAAGAC 1 cut(s) 1507
BstX2I RGATCY 1 cut(s) 1492
BstYI RGATCY 1 cut(s) 1492
BsuRI GGCC 5 cut(s) 97, 352, 819, 1290, 1669
BtgI CCRYGG 1 cut(s) 569
BtsCI GGATG 2 cut(s) 835, 1717
BtsIMutI CAGTG 2 cut(s) 84, 258
Cac8I GCNNGC 7 cut(s) 265, 350, 354, 915, 1144, 1288, 1398
CaiI CAGNNNCTG 1 cut(s) 242
CfoI GCGC 2 cut(s) 50, 440
Cfr10I RCCGGY 1 cut(s) 1201
Cfr13I GGNCC 6 cut(s) 131, 536, 744, 818, 1010, 1667
CseI GACGC 1 cut(s) 722
Csp6I GTAC 2 cut(s) 328, 1330
CviAII CATG 3 cut(s) 215, 570, 1151
CviQI GTAC 2 cut(s) 328, 1330
DdeI CTNAG 3 cut(s) 141, 775, 1305
DpnI GATC 5 cut(s) 791, 1022, 1335, 1494, 1543
DpnII GATC 5 cut(s) 789, 1020, 1333, 1492, 1541
EaeI YGGCCR 1 cut(s) 95
Eam1104I CTCTTC 2 cut(s) 909, 954
EarI CTCTTC 2 cut(s) 909, 954
Eco130I CCWWGG 4 cut(s) 11, 569, 618, 1407
Eco147I AGGCCT 1 cut(s) 352
Eco24I GRGCYC 1 cut(s) 1739
Eco47I GGWCC 4 cut(s) 131, 536, 744, 1010
Eco47III AGCGCT 1 cut(s) 439
Eco91I GGTNACC 1 cut(s) 1197
EcoO65I GGTNACC 1 cut(s) 1197
EcoRI GAATTC 1 cut(s) 1549
EcoRII CCWGG 4 cut(s) 516, 752, 1057, 1318
EcoT14I CCWWGG 4 cut(s) 11, 569, 618, 1407
EcoT38I GRGCYC 1 cut(s) 1739
ErhI CCWWGG 4 cut(s) 11, 569, 618, 1407
Esp3I CGTCTC 1 cut(s) 705
FaeI CATG 3 cut(s) 218, 573, 1154
FaqI GGGAC 4 cut(s) 666, 757, 1011, 1144
FatI CATG 3 cut(s) 214, 569, 1150
FauI CCCGC 1 cut(s) 293
FauNDI CATATG 1 cut(s) 985
FbaI TGATCA 1 cut(s) 789
FblI GTMKAC 1 cut(s) 411
Fnu4HI GCNGC 9 cut(s) 240, 346, 381, 588, 852, 1053, 1077, 1167, 1206
FokI GGATG 2 cut(s) 842, 1724
FriOI GRGCYC 1 cut(s) 1739
Fsp4HI GCNGC 9 cut(s) 240, 346, 381, 588, 852, 1053, 1077, 1167, 1206
FspBI CTAG 3 cut(s) 1073, 1257, 1419
GlaI GCGC 2 cut(s) 49, 439
GluI GCNGC 9 cut(s) 240, 346, 381, 588, 852, 1053, 1077, 1167, 1206
GsaI CCCAGC 1 cut(s) 246
GsuI CTGGAG 3 cut(s) 361, 736, 1041
HaeII RGCGCY 2 cut(s) 51, 441
HaeIII GGCC 5 cut(s) 97, 352, 819, 1290, 1669
HapII CCGG 4 cut(s) 539, 679, 855, 1202
HgaI GACGC 1 cut(s) 722
HhaI GCGC 2 cut(s) 50, 440
Hin1II CATG 3 cut(s) 218, 573, 1154
Hin6I GCGC 2 cut(s) 48, 438
HinP1I GCGC 2 cut(s) 48, 438
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HinfI GANTC 8 cut(s) 101, 191, 203, 464, 749, 1563, 1587, 1603
HpaII CCGG 4 cut(s) 539, 679, 855, 1202
HphI GGTGA 3 cut(s) 607, 1178, 1191
Hpy166II GTNNAC 5 cut(s) 328, 412, 1330, 1370, 1388
Hpy188I TCNGA 3 cut(s) 106, 1020, 1497
Hpy188III TCNNGA 5 cut(s) 21, 433, 928, 1337, 1732
Hpy8I GTNNAC 5 cut(s) 328, 412, 1330, 1370, 1388
Hpy99I CGWCG 3 cut(s) 74, 929, 1549
HpyAV CCTTC 3 cut(s) 527, 1311, 1479
HpyCH4III ACNGT 6 cut(s) 37, 253, 407, 553, 638, 1528
HpyCH4IV ACGT 3 cut(s) 316, 924, 1173
HpyF10VI GCNNNNNNNGC 7 cut(s) 236, 264, 320, 345, 1166, 1274, 1283
HpyF3I CTNAG 3 cut(s) 141, 775, 1305
HpySE526I ACGT 3 cut(s) 316, 924, 1173
Hsp92II CATG 3 cut(s) 218, 573, 1154
HspAI GCGC 2 cut(s) 48, 438
Ksp22I TGATCA 1 cut(s) 789
Kzo9I GATC 5 cut(s) 789, 1020, 1333, 1492, 1541
Lsp1109I GCAGC 7 cut(s) 251, 357, 574, 863, 1088, 1153, 1192
LweI GCATC 6 cut(s) 109, 239, 355, 382, 926, 1706
MaeI CTAG 3 cut(s) 1073, 1257, 1419
MaeII ACGT 3 cut(s) 316, 924, 1173
MaeIII GTNAC 6 cut(s) 527, 553, 632, 1197, 1528, 1654
MalI GATC 5 cut(s) 791, 1022, 1335, 1494, 1543
MboI GATC 5 cut(s) 789, 1020, 1333, 1492, 1541
MboII GAAGA 6 cut(s) 374, 791, 794, 896, 971, 1507
MfeI CAATTG 2 cut(s) 474, 626
MflI RGATCY 1 cut(s) 1492
MhlI GDGCHC 3 cut(s) 993, 1148, 1739
MlsI TGGCCA 1 cut(s) 97
MluNI TGGCCA 1 cut(s) 97
MlyI GAGTC 6 cut(s) 110, 197, 200, 743, 1572, 1581
MmeI TCCRAC 2 cut(s) 84, 1519
MnlI CCTC 6 cut(s) 144, 290, 643, 686, 912, 1003
Mox20I TGGCCA 1 cut(s) 97
MscI TGGCCA 1 cut(s) 97
MseI TTAA 4 cut(s) 39, 698, 966, 1762
Msp20I TGGCCA 1 cut(s) 97
MspA1I CMGCKG 1 cut(s) 242
MspI CCGG 4 cut(s) 539, 679, 855, 1202
MspR9I CCNGG 6 cut(s) 518, 540, 680, 754, 1059, 1320
MunI CAATTG 2 cut(s) 474, 626
Mva1269I GAATGC 1 cut(s) 383
MvaI CCWGG 4 cut(s) 518, 754, 1059, 1320
MwoI GCNNNNNNNGC 7 cut(s) 236, 264, 320, 345, 1166, 1274, 1283
NciI CCSGG 2 cut(s) 540, 680
NcoI CCATGG 1 cut(s) 569
NdeI CATATG 1 cut(s) 985
NdeII GATC 5 cut(s) 789, 1020, 1333, 1492, 1541
NlaIII CATG 3 cut(s) 218, 573, 1154
NmuCI GTSAC 3 cut(s) 553, 1197, 1654
PceI AGGCCT 1 cut(s) 352
PcsI WCGNNNNNNNCGW 1 cut(s) 528
PctI GAATGC 1 cut(s) 383
PfeI GAWTC 2 cut(s) 464, 1603
PflFI GACNNNGTC 1 cut(s) 407
PflMI CCANNNNNTGG 2 cut(s) 1094, 1319
PfoI TCCNGGA 2 cut(s) 538, 1057
PkrI GCNGC 9 cut(s) 241, 347, 382, 589, 853, 1054, 1078, 1168, 1207
PleI GAGTC 6 cut(s) 109, 197, 199, 743, 1571, 1581
PpsI GAGTC 6 cut(s) 109, 197, 199, 743, 1571, 1581
Ppu21I YACGTR 1 cut(s) 317
PshBI ATTAAT 1 cut(s) 966
Psp6I CCWGG 4 cut(s) 516, 752, 1057, 1318
PspEI GGTNACC 1 cut(s) 1197
PspFI CCCAGC 1 cut(s) 242
PspGI CCWGG 4 cut(s) 516, 752, 1057, 1318
PspPI GGNCC 6 cut(s) 131, 536, 744, 818, 1010, 1667
PstNI CAGNNNCTG 1 cut(s) 242
PsuI RGATCY 1 cut(s) 1492
PsyI GACNNNGTC 1 cut(s) 407
PvuII CAGCTG 1 cut(s) 242
RsaI GTAC 2 cut(s) 329, 1331
RsaNI GTAC 2 cut(s) 328, 1330
SalI GTCGAC 1 cut(s) 410
SaqAI TTAA 4 cut(s) 39, 698, 966, 1762
SatI GCNGC 9 cut(s) 240, 346, 381, 588, 852, 1053, 1077, 1167, 1206
Sau3AI GATC 5 cut(s) 789, 1020, 1333, 1492, 1541
Sau96I GGNCC 6 cut(s) 131, 536, 744, 818, 1010, 1667
SchI GAGTC 6 cut(s) 110, 197, 200, 743, 1572, 1581
ScrFI CCNGG 6 cut(s) 518, 540, 680, 754, 1059, 1320
SduI GDGCHC 3 cut(s) 993, 1148, 1739
SfaNI GCATC 6 cut(s) 109, 239, 355, 382, 926, 1706
SfcI CTRYAG 1 cut(s) 1155
SinI GGWCC 4 cut(s) 131, 536, 744, 1010
SmlI CTYRAG 2 cut(s) 896, 1037
SmoI CTYRAG 2 cut(s) 896, 1037
SpeI ACTAGT 1 cut(s) 1418
SseBI AGGCCT 1 cut(s) 352
SsiI CCGC 5 cut(s) 300, 380, 604, 1052, 1233
SspMI CTAG 3 cut(s) 1073, 1257, 1419
StuI AGGCCT 1 cut(s) 352
StyD4I CCNGG 6 cut(s) 516, 538, 678, 752, 1057, 1318
StyI CCWWGG 4 cut(s) 11, 569, 618, 1407
TaaI ACNGT 6 cut(s) 37, 253, 407, 553, 638, 1528
TaiI ACGT 3 cut(s) 319, 927, 1176
TaqI TCGA 3 cut(s) 387, 411, 1547
TaqII GACCGA 1 cut(s) 761
TatI WGTACW 1 cut(s) 327
TauI GCSGC 2 cut(s) 383, 1055
TfiI GAWTC 2 cut(s) 464, 1603
Tru1I TTAA 4 cut(s) 39, 698, 966, 1762
Tru9I TTAA 4 cut(s) 39, 698, 966, 1762
TscAI CASTG 2 cut(s) 91, 258
TseFI GTSAC 3 cut(s) 553, 1197, 1654
TseI GCWGC 7 cut(s) 239, 345, 587, 851, 1076, 1166, 1205
Tsp45I GTSAC 3 cut(s) 553, 1197, 1654
TspDTI ATGAA 5 cut(s) 456, 601, 903, 1356, 1416
TspGWI ACGGA 1 cut(s) 1296
TspRI CASTG 2 cut(s) 91, 258
Tth111I GACNNNGTC 1 cut(s) 407
Van91I CCANNNNNTGG 2 cut(s) 1094, 1319
VpaK11BI GGWCC 4 cut(s) 131, 536, 744, 1010
VspI ATTAAT 1 cut(s) 966
XapI RAATTY 5 cut(s) 90, 1271, 1499, 1549, 1684
XcmI CCANNNNNNNNNTGG 2 cut(s) 144, 625
XmiI GTMKAC 1 cut(s) 411
XspI CTAG 3 cut(s) 1073, 1257, 1419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.