Rw1G031960

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000390
Physical Location & Seq
Reverse (-)
60157357 .. 60161227
3871 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G031960.1

Sequence Viewer

Length: 2265 bp
ATGGTCAATTATCCCATAATTTTATGTTGCAGCACTTCCATTTTAAGAACCGTTTTTGGGTTTATCCTATTCTTAATGAAATCCACGATATTTACTATTGTTTTGAATAAAATTACATGCATCCAGAATTGTACGTACGCTTATGTTGTGTATATGGGCGGCAAGCCAAAGAATGGGGTACCCATATTACCTGATCTTCATGTGAATATGCTACAAGACGTAGTTGACAGTAGCAACATTGATATTGCACATGAAGATCTGCTTCTTCACAGCTACAAGAGAAGTTTCAATGGATTTGCTGCAATGCTCACAGAGCAAGAAGCACAAAAAATGATTGGAATGGATGGTGTAGTGTCTGTCTTCCCAAGCAAACAGAGGAGCGTCAAAACAACAAAGTCATGGAACTTTGTTGGGTTTCCAGAAACAGTTAAGAGAGGCGCCCTTGAAAGGGATATCATTGTTGGTGTGATAGACAGTGGAATTTGGCCAGAGTCGGACAGCTTTAGTGATGTTGGTTTTGGTCCCCCACCCAAGAAATGGAAAGGCAAATGTCAAGGCAATGGAAATCTTACTTGTAACAATAAAATCATTGGAGCACAATATTATCATGCTTCTCAATCATTCAAAGAAGATGTTGAGTCTCCAAGAGACACAAACGGTCATGGAACTCACACTGCATCAACAGCAGCTGGGAACACAGTGATCAAGGTGAGCTTTAATGGTTTAGGGTCGGGAACAGCAAGAGGAGGGGTGCCATCAGCGCGTATTGCTATGTACAAAGCATGTTGGGGCAGCAGTGGCAGTTGTGACGATGCTGATGTTCTAGCCGCATTCGATGATGCCATTGCTGACGGTGTAGACATTCTCTCTGTTTCCCTCGGGGGCACTATTCCATTTGATTATTTGAAGGATTCATTTGCAATTGGGTCATTTCACGCTACCAGAAATGGGATATTAGTTTCTATGGCCGCTGGTAACGAAGGTCCGGGCGCAAAAACTGTGACAAACTTTGCACCATGGCAACTTTCTGTGGCTGCTACCACCATAAACCGCCAATTCATCACCAAGGTTCAATTAGGTAACGGTAAAATCTATGAGGGACTTTTACCAAACATATATGGTCTCCGGGGTAAATTCTATCCTTTAATTTATGGTGGGGACGCGCCCAATACAAAAGCAGGTGTTGACGGGAGTACCTCTAGGTTTTGTGGAAAAGATGACGTAGACGAAGATTTGATCAAAGGTAAAATTGTGTTGTGTGATGGCCCTATGGATGAGGCTGGGGTTGGGGCGATATTGGGTGGCGCAGCCGGAATTGTTTTGACAGGAAAAAAAGTCAACGATGAACTGCTTGGCCCTTATCCGGTGCCTACATCTTTCGTCGGGTTGGAAGAAAACAGCGACATTTACAAATACATAAATTCAACAAGGAATGCAACTGCAACTATATGGAAAAGTGAAGAGATTAGTGATGTATTGGCTCCATACGTGCCATCCTACTCATCAAGGGGCCCAAATCCGATCAATCCCAACATTCTCAAGCCAGATTTAGCGGCTCCAGGAACTTACATTCTGGCAGCATATCCCCCAAGTGGTGGTGGCAGTAGAGCTAGATACTATATAGAAACTGGGACATCAATGGCATGCCCCCATGCTACCGCCGTAGCTGCATACGTCAAATCATTTCACCCTAAATGGTCACCAGCTGCTATCCAATCGGCTCTCATCACTACTGCTAAACCTATGAGTGCCAAAACTAGCCCTGACGCTGAATTTGCATACGGAGCTGGCCTAATAAACCCTTCTAGGGCGCCATATCCTGGTTTGGTGTATGATCTTGATGGACAAGACTACATAGATTTTTTGTGTTCACAAGGATACAATGGTAAACTATTGCAAGCCATAACCATGGACAAAACTAGTTGCTCATCAAAATCTATTAATAGAACAGCTAATGACATGAACTATCCTTCTTTTGCACTTTCCATCAAGGATTCGAAATTTGTCAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCGTCAAAAGCCACATACAGAGCTAAAACGGTGGCTCCATTGGGACTCAAAATCAATGTGAATCCAAGTGTGCTATCGTTCACATCTCTTGGGCAAAAGAAATCTTTTGTTGTCACCATAAAAGGGTCGATTAAGAAATCAAATCTAATCTCTGCGTCTTTGGTGTGGGATGATGGTGCTTTCCAAGTCAGGAGCCCCATTGTTGTCTATGTTGTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

754

Amino Acids

80.55

Weight (kDa)

8.62

Isoelectric Point (pI)

33.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 48 - 127 2.1e-12 Peptidase inhibitor I9
Peptidase_S8 PF00082 150 - 597 1e-42 Subtilase family
fn3_6 PF17766 653 - 750 2.9e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1169
Acc36I ACCTGC 1 cut(s) 1169
Acc65I GGTACC 1 cut(s) 178
AccB1I GGYRCC 5 cut(s) 178, 437, 751, 1366, 1810
AccB7I CCANNNNNTGG 4 cut(s) 173, 537, 1595, 1820
AccI GTMKAC 2 cut(s) 858, 1224
AccII CGCG 2 cut(s) 763, 1163
AciI CCGC 6 cut(s) 159, 828, 969, 1051, 1553, 1659
AclWI GGATC 1 cut(s) 2050
AcoI YGGCCR 2 cut(s) 485, 966
AcsI RAATTY 5 cut(s) 480, 1133, 1420, 1772, 2000
AcyI GRCGYC 2 cut(s) 438, 1811
AfaI GTAC 5 cut(s) 133, 137, 180, 776, 1195
AgsI TTSAA 7 cut(s) 106, 289, 446, 625, 907, 1073, 1425
AhlI ACTAGT 1 cut(s) 1919
AjnI CCWGG 2 cut(s) 1558, 1819
Alw21I GWGCWC 1 cut(s) 598
Alw26I GTCTC 3 cut(s) 642, 645, 1127
AlwI GGATC 1 cut(s) 2050
AlwNI CAGNNNCTG 1 cut(s) 689
Ama87I CYCGRG 1 cut(s) 878
AoxI GGCC 6 cut(s) 485, 966, 1264, 1354, 1510, 1789
ApaI GGGCCC 1 cut(s) 1514
ApeKI GCWGC 9 cut(s) 30, 299, 686, 792, 1034, 1307, 1577, 1667, 1706
ApoI RAATTY 5 cut(s) 480, 1133, 1420, 1772, 2000
ArsI GACNNNNNNTTYG 2 cut(s) 1757, 1789
AseI ATTAAT 1 cut(s) 1941
Asp718I GGTACC 1 cut(s) 178
AspLEI GCGC 6 cut(s) 440, 763, 992, 1165, 1307, 1813
AspS9I GGNCC 6 cut(s) 521, 983, 1265, 1355, 1510, 1511
AsuC2I CCSGG 2 cut(s) 987, 1127
AsuHPI GGTGA 5 cut(s) 721, 1054, 1679, 1692, 2149
AsuII TTCGAA 1 cut(s) 1997
AvaI CYCGRG 1 cut(s) 878
AvaII GGWCC 2 cut(s) 521, 983
BaeGI GKGCMC 2 cut(s) 887, 1514
BaeI ACNNNNGTAYC 4 cut(s) 1177, 1210, 1870, 1903
BalI TGGCCA 1 cut(s) 487
BanI GGYRCC 5 cut(s) 178, 437, 751, 1366, 1810
BanII GRGCYC 2 cut(s) 1514, 2240
BbsI GAAGAC 2 cut(s) 352, 2008
Bbv12I GWGCWC 1 cut(s) 598
BbvI GCAGC 9 cut(s) 42, 286, 698, 804, 1021, 1319, 1589, 1654, 1693
BccI CCATC 7 cut(s) 338, 763, 1256, 1501, 1835, 1994, 2210
BceAI ACGGC 1 cut(s) 1646
BciT130I CCWGG 2 cut(s) 1560, 1821
BciVI GTATCC 1 cut(s) 1871
BclI TGATCA 2 cut(s) 702, 1236
BcnI CCSGG 2 cut(s) 987, 1127
BcoDI GTCTC 3 cut(s) 642, 645, 1127
BcuI ACTAGT 1 cut(s) 1919
BfaI CTAG 6 cut(s) 824, 1200, 1611, 1758, 1806, 1920
BfoI RGCGCY 2 cut(s) 441, 1814
BfuAI ACCTGC 1 cut(s) 1169
BfuI GTATCC 1 cut(s) 1871
BglII AGATCT 1 cut(s) 256
Bme1390I CCNGG 4 cut(s) 987, 1127, 1560, 1821
Bme18I GGWCC 2 cut(s) 521, 983
BmeT110I CYCGRG 1 cut(s) 878
BmgT120I GGNCC 6 cut(s) 521, 983, 1265, 1355, 1510, 1511
BmrFI CCNGG 4 cut(s) 987, 1127, 1560, 1821
BmrI ACTGGG 1 cut(s) 1638
BmsI GCATC 4 cut(s) 129, 686, 802, 829
BmuI ACTGGG 1 cut(s) 1638
BpiI GAAGAC 2 cut(s) 352, 2008
BpmI CTGGAG 1 cut(s) 1542
Bpu14I TTCGAA 1 cut(s) 1997
BpuEI CTTGAG 1 cut(s) 1523
BpuMI CCSGG 2 cut(s) 987, 1127
BsaAI YACGTR 2 cut(s) 135, 1489
BsaHI GRCGYC 2 cut(s) 438, 1811
BsaI GGTCTC 1 cut(s) 1127
BsaJI CCNNGG 5 cut(s) 877, 1016, 1065, 1126, 1908
BsaWI WCCGGW 1 cut(s) 1363
BsaXI ACNNNNNCTCC 2 cut(s) 2062, 2092
Bse1I ACTGG 1 cut(s) 1633
Bse3DI GCAATG 3 cut(s) 309, 565, 843
BseBI CCWGG 2 cut(s) 1560, 1821
BseDI CCNNGG 5 cut(s) 877, 1016, 1065, 1126, 1908
BseGI GGATG 5 cut(s) 120, 349, 1279, 1493, 2218
BseMI GCAATG 3 cut(s) 309, 565, 843
BseNI ACTGG 1 cut(s) 1633
BseRI GAGGAG 2 cut(s) 391, 759
BseSI GKGCMC 2 cut(s) 887, 1514
BseXI GCAGC 9 cut(s) 42, 286, 698, 804, 1021, 1319, 1589, 1654, 1693
BseYI CCCAGC 2 cut(s) 689, 1280
Bsh1236I CGCG 2 cut(s) 763, 1163
BshFI GGCC 6 cut(s) 487, 968, 1266, 1356, 1512, 1791
BshNI GGYRCC 5 cut(s) 178, 437, 751, 1366, 1810
BsiHKAI GWGCWC 1 cut(s) 598
BsiHKCI CYCGRG 1 cut(s) 878
BsiSI CCGG 4 cut(s) 986, 1126, 1311, 1364
BsiWI CGTACG 1 cut(s) 135
BslFI GGGAC 5 cut(s) 507, 1113, 1172, 1645, 2100
BsmAI GTCTC 3 cut(s) 642, 645, 1127
BsmFI GGGAC 5 cut(s) 507, 1113, 1172, 1645, 2100
BsmI GAATGC 2 cut(s) 830, 1438
BsnI GGCC 6 cut(s) 487, 968, 1266, 1356, 1512, 1791
Bso31I GGTCTC 1 cut(s) 1127
BsoBI CYCGRG 1 cut(s) 878
Bsp119I TTCGAA 1 cut(s) 1997
Bsp120I GGGCCC 1 cut(s) 1510
Bsp1286I GDGCHC 4 cut(s) 598, 887, 1514, 2240
Bsp1407I TGTACA 1 cut(s) 774
Bsp143I GATC 7 cut(s) 193, 256, 702, 1236, 1521, 1834, 2042
Bsp19I CCATGG 2 cut(s) 1016, 1908
BspACI CCGC 6 cut(s) 159, 828, 969, 1051, 1553, 1659
BspANI GGCC 6 cut(s) 487, 968, 1266, 1356, 1512, 1791
BspFNI CGCG 2 cut(s) 763, 1163
BspMI ACCTGC 1 cut(s) 1169
BspPI GGATC 1 cut(s) 2050
BspT104I TTCGAA 1 cut(s) 1997
BspT107I GGYRCC 5 cut(s) 178, 437, 751, 1366, 1810
BspTNI GGTCTC 1 cut(s) 1127
BsrDI GCAATG 3 cut(s) 309, 565, 843
BsrGI TGTACA 1 cut(s) 774
BsrI ACTGG 1 cut(s) 1633
BssECI CCNNGG 5 cut(s) 877, 1016, 1065, 1126, 1908
BssMI GATC 7 cut(s) 193, 256, 702, 1236, 1521, 1834, 2042
BssNI GRCGYC 2 cut(s) 438, 1811
BssT1I CCWWGG 3 cut(s) 1016, 1065, 1908
Bst2UI CCWGG 2 cut(s) 1560, 1821
Bst6I CTCTTC 1 cut(s) 1455
BstACI GRCGYC 2 cut(s) 438, 1811
BstAUI TGTACA 1 cut(s) 774
BstBAI YACGTR 2 cut(s) 135, 1489
BstBI TTCGAA 1 cut(s) 1997
BstC8I GCNNGC 4 cut(s) 164, 1645, 1789, 1899
BstDSI CCRYGG 2 cut(s) 1016, 1908
BstEII GGTNACC 1 cut(s) 1698
BstF5I GGATG 5 cut(s) 120, 349, 1279, 1493, 2218
BstFNI CGCG 2 cut(s) 763, 1163
BstH2I RGCGCY 2 cut(s) 441, 1814
BstHHI GCGC 6 cut(s) 440, 763, 992, 1165, 1307, 1813
BstKTI GATC 7 cut(s) 196, 259, 705, 1239, 1524, 1837, 2045
BstMAI GTCTC 3 cut(s) 642, 645, 1127
BstMBI GATC 7 cut(s) 193, 256, 702, 1236, 1521, 1834, 2042
BstMWI GCNNNNNNNGC 8 cut(s) 313, 683, 760, 767, 798, 1667, 1775, 1784
BstNI CCWGG 2 cut(s) 1560, 1821
BstNSI RCATGY 3 cut(s) 120, 786, 1647
BstPI GGTNACC 1 cut(s) 1698
BstSCI CCNGG 4 cut(s) 985, 1125, 1558, 1819
BstSLI GKGCMC 2 cut(s) 887, 1514
BstSNI TACGTA 1 cut(s) 135
BstUI CGCG 2 cut(s) 763, 1163
BstV1I GCAGC 9 cut(s) 42, 286, 698, 804, 1021, 1319, 1589, 1654, 1693
BstV2I GAAGAC 2 cut(s) 352, 2008
BstX2I RGATCY 1 cut(s) 256
BstXI CCANNNNNNTGG 1 cut(s) 1909
BstYI RGATCY 1 cut(s) 256
BsuI GTATCC 1 cut(s) 1871
BsuRI GGCC 6 cut(s) 487, 968, 1266, 1356, 1512, 1791
BtgI CCRYGG 2 cut(s) 1016, 1908
BtsCI GGATG 5 cut(s) 120, 349, 1279, 1493, 2218
BtsI GCAGTG 2 cut(s) 672, 802
BtsIMutI CAGTG 4 cut(s) 481, 672, 705, 802
BveI ACCTGC 1 cut(s) 1169
Cac8I GCNNGC 4 cut(s) 164, 1645, 1789, 1899
CaiI CAGNNNCTG 1 cut(s) 689
CfoI GCGC 6 cut(s) 440, 763, 992, 1165, 1307, 1813
Cfr13I GGNCC 6 cut(s) 521, 983, 1265, 1355, 1510, 1511
CseI GACGC 4 cut(s) 370, 1169, 1775, 2187
Csp6I GTAC 5 cut(s) 132, 136, 179, 775, 1194
CviQI GTAC 5 cut(s) 132, 136, 179, 775, 1194
DinI GGCGCC 2 cut(s) 439, 1812
DpnI GATC 7 cut(s) 195, 258, 704, 1238, 1523, 1836, 2044
DpnII GATC 7 cut(s) 193, 256, 702, 1236, 1521, 1834, 2042
EaeI YGGCCR 2 cut(s) 485, 966
Eam1104I CTCTTC 1 cut(s) 1455
EarI CTCTTC 1 cut(s) 1455
Eco105I TACGTA 1 cut(s) 135
Eco130I CCWWGG 3 cut(s) 1016, 1065, 1908
Eco24I GRGCYC 2 cut(s) 1514, 2240
Eco31I GGTCTC 1 cut(s) 1127
Eco32I GATATC 1 cut(s) 454
Eco47I GGWCC 2 cut(s) 521, 983
Eco88I CYCGRG 1 cut(s) 878
Eco91I GGTNACC 1 cut(s) 1698
EcoO109I RGGNCCY 1 cut(s) 1510
EcoO65I GGTNACC 1 cut(s) 1698
EcoRII CCWGG 2 cut(s) 1558, 1819
EcoRV GATATC 1 cut(s) 454
EcoT14I CCWWGG 3 cut(s) 1016, 1065, 1908
EcoT22I ATGCAT 1 cut(s) 122
EcoT38I GRGCYC 2 cut(s) 1514, 2240
EgeI GGCGCC 2 cut(s) 439, 1812
EheI GGCGCC 2 cut(s) 439, 1812
ErhI CCWWGG 3 cut(s) 1016, 1065, 1908
FalI AAGNNNNNCTT 4 cut(s) 246, 278, 698, 730
FaqI GGGAC 5 cut(s) 507, 1113, 1172, 1645, 2100
FbaI TGATCA 2 cut(s) 702, 1236
FblI GTMKAC 2 cut(s) 858, 1224
FokI GGATG 5 cut(s) 107, 356, 1286, 1480, 2225
FriOI GRGCYC 2 cut(s) 1514, 2240
FspBI CTAG 6 cut(s) 824, 1200, 1611, 1758, 1806, 1920
GlaI GCGC 6 cut(s) 439, 762, 991, 1164, 1306, 1812
GsaI CCCAGC 2 cut(s) 693, 1284
GsuI CTGGAG 1 cut(s) 1542
HaeII RGCGCY 2 cut(s) 441, 1814
HaeIII GGCC 6 cut(s) 487, 968, 1266, 1356, 1512, 1791
HapII CCGG 4 cut(s) 986, 1126, 1311, 1364
HgaI GACGC 4 cut(s) 370, 1169, 1775, 2187
HhaI GCGC 6 cut(s) 440, 763, 992, 1165, 1307, 1813
Hin1I GRCGYC 2 cut(s) 438, 1811
Hin6I GCGC 6 cut(s) 438, 761, 990, 1163, 1305, 1811
HinP1I GCGC 6 cut(s) 438, 761, 990, 1163, 1305, 1811
HincII GTYRAC 3 cut(s) 226, 1186, 1339
HindII GTYRAC 3 cut(s) 226, 1186, 1339
HinfI GANTC 6 cut(s) 491, 638, 911, 1994, 2088, 2104
HpaII CCGG 4 cut(s) 986, 1126, 1311, 1364
HphI GGTGA 5 cut(s) 721, 1054, 1679, 1692, 2149
Hpy166II GTNNAC 8 cut(s) 226, 859, 1186, 1225, 1339, 1871, 1889, 2124
Hpy188I TCNGA 2 cut(s) 496, 1521
Hpy188III TCNNGA 5 cut(s) 124, 419, 732, 1838, 2233
Hpy8I GTNNAC 8 cut(s) 226, 859, 1186, 1225, 1339, 1871, 1889, 2124
Hpy99I CGWCG 1 cut(s) 1385
HpyAV CCTTC 4 cut(s) 901, 974, 1812, 1980
HpyCH4IV ACGT 5 cut(s) 134, 219, 1221, 1488, 1674
HpyF10VI GCNNNNNNNGC 8 cut(s) 313, 683, 760, 767, 798, 1667, 1775, 1784
HpySE526I ACGT 5 cut(s) 134, 219, 1221, 1488, 1674
Hsp92I GRCGYC 2 cut(s) 438, 1811
HspAI GCGC 6 cut(s) 438, 761, 990, 1163, 1305, 1811
KasI GGCGCC 2 cut(s) 437, 1810
KpnI GGTACC 1 cut(s) 182
Ksp22I TGATCA 2 cut(s) 702, 1236
Kzo9I GATC 7 cut(s) 193, 256, 702, 1236, 1521, 1834, 2042
LmnI GCTCC 7 cut(s) 378, 593, 1486, 1561, 1784, 2083, 2235
Lsp1109I GCAGC 9 cut(s) 42, 286, 698, 804, 1021, 1319, 1589, 1654, 1693
LweI GCATC 4 cut(s) 129, 686, 802, 829
MaeI CTAG 6 cut(s) 824, 1200, 1611, 1758, 1806, 1920
MaeII ACGT 5 cut(s) 134, 219, 1221, 1488, 1674
MaeIII GTNAC 8 cut(s) 575, 806, 974, 1000, 1079, 1698, 2029, 2155
MalI GATC 7 cut(s) 195, 258, 704, 1238, 1523, 1836, 2044
MboI GATC 7 cut(s) 193, 256, 702, 1236, 1521, 1834, 2042
MboII GAAGA 9 cut(s) 188, 257, 266, 352, 641, 1241, 1403, 1472, 2008
MfeI CAATTG 1 cut(s) 921
MflI RGATCY 1 cut(s) 256
MhlI GDGCHC 4 cut(s) 598, 887, 1514, 2240
MlsI TGGCCA 1 cut(s) 487
MluNI TGGCCA 1 cut(s) 487
Mly113I GGCGCC 2 cut(s) 438, 1811
MlyI GAGTC 3 cut(s) 500, 647, 2082
MmeI TCCRAC 3 cut(s) 474, 1368, 2020
MnlI CCTC 8 cut(s) 369, 428, 737, 740, 887, 1090, 1207, 1270
Mox20I TGGCCA 1 cut(s) 487
Mph1103I ATGCAT 1 cut(s) 122
MscI TGGCCA 1 cut(s) 487
MseI TTAA 8 cut(s) 44, 74, 429, 717, 1145, 1941, 2175, 2263
MslI CAYNNNNRTG 1 cut(s) 1907
Msp20I TGGCCA 1 cut(s) 487
MspA1I CMGCKG 3 cut(s) 689, 971, 1706
MspI CCGG 4 cut(s) 986, 1126, 1311, 1364
MspR9I CCNGG 4 cut(s) 987, 1127, 1560, 1821
MunI CAATTG 1 cut(s) 921
Mva1269I GAATGC 2 cut(s) 830, 1438
MvaI CCWGG 2 cut(s) 1560, 1821
MvnI CGCG 2 cut(s) 763, 1163
MwoI GCNNNNNNNGC 8 cut(s) 313, 683, 760, 767, 798, 1667, 1775, 1784
NarI GGCGCC 2 cut(s) 438, 1811
NciI CCSGG 2 cut(s) 987, 1127
NcoI CCATGG 2 cut(s) 1016, 1908
NdeII GATC 7 cut(s) 193, 256, 702, 1236, 1521, 1834, 2042
NmuCI GTSAC 5 cut(s) 806, 1000, 1698, 2029, 2155
NsiI ATGCAT 1 cut(s) 122
NspI RCATGY 3 cut(s) 120, 786, 1647
NspV TTCGAA 1 cut(s) 1997
PaeI GCATGC 1 cut(s) 1647
PaqCI CACCTGC 1 cut(s) 1169
PctI GAATGC 2 cut(s) 830, 1438
PfeI GAWTC 3 cut(s) 911, 1994, 2104
Pfl23II CGTACG 1 cut(s) 135
PflMI CCANNNNNTGG 4 cut(s) 173, 537, 1595, 1820
PfoI TCCNGGA 1 cut(s) 1558
PleI GAGTC 3 cut(s) 499, 646, 2082
PluTI GGCGCC 2 cut(s) 441, 1814
PpsI GAGTC 3 cut(s) 499, 646, 2082
Ppu21I YACGTR 2 cut(s) 135, 1489
PshBI ATTAAT 1 cut(s) 1941
Psp6I CCWGG 2 cut(s) 1558, 1819
PspEI GGTNACC 1 cut(s) 1698
PspFI CCCAGC 2 cut(s) 689, 1280
PspGI CCWGG 2 cut(s) 1558, 1819
PspLI CGTACG 1 cut(s) 135
PspOMI GGGCCC 1 cut(s) 1510
PspPI GGNCC 6 cut(s) 521, 983, 1265, 1355, 1510, 1511
PstNI CAGNNNCTG 1 cut(s) 689
PsuI RGATCY 1 cut(s) 256
PvuII CAGCTG 2 cut(s) 689, 1706
RsaI GTAC 5 cut(s) 133, 137, 180, 776, 1195
RsaNI GTAC 5 cut(s) 132, 136, 179, 775, 1194
RseI CAYNNNNRTG 1 cut(s) 1907
SaqAI TTAA 8 cut(s) 44, 74, 429, 717, 1145, 1941, 2175, 2263
Sau3AI GATC 7 cut(s) 193, 256, 702, 1236, 1521, 1834, 2042
Sau96I GGNCC 6 cut(s) 521, 983, 1265, 1355, 1510, 1511
SchI GAGTC 3 cut(s) 500, 647, 2082
ScrFI CCNGG 4 cut(s) 987, 1127, 1560, 1821
SduI GDGCHC 4 cut(s) 598, 887, 1514, 2240
SfaNI GCATC 4 cut(s) 129, 686, 802, 829
SfoI GGCGCC 2 cut(s) 439, 1812
SfuI TTCGAA 1 cut(s) 1997
SinI GGWCC 2 cut(s) 521, 983
SmiMI CAYNNNNRTG 1 cut(s) 1907
SmlI CTYRAG 1 cut(s) 1538
SmoI CTYRAG 1 cut(s) 1538
SnaBI TACGTA 1 cut(s) 135
SpeI ACTAGT 1 cut(s) 1919
SphI GCATGC 1 cut(s) 1647
SsiI CCGC 6 cut(s) 159, 828, 969, 1051, 1553, 1659
SspDI GGCGCC 2 cut(s) 437, 1810
SspI AATATT 1 cut(s) 602
SspMI CTAG 6 cut(s) 824, 1200, 1611, 1758, 1806, 1920
StyD4I CCNGG 4 cut(s) 985, 1125, 1558, 1819
StyI CCWWGG 3 cut(s) 1016, 1065, 1908
TaiI ACGT 5 cut(s) 137, 222, 1224, 1491, 1677
TaqI TCGA 3 cut(s) 834, 1997, 2171
TatI WGTACW 1 cut(s) 774
TauI GCSGC 4 cut(s) 162, 830, 971, 1556
TfiI GAWTC 3 cut(s) 911, 1994, 2104
Tru1I TTAA 8 cut(s) 44, 74, 429, 717, 1145, 1941, 2175, 2263
Tru9I TTAA 8 cut(s) 44, 74, 429, 717, 1145, 1941, 2175, 2263
TscAI CASTG 4 cut(s) 481, 679, 705, 802
TseFI GTSAC 5 cut(s) 806, 1000, 1698, 2029, 2155
TseI GCWGC 9 cut(s) 30, 299, 686, 792, 1034, 1307, 1577, 1667, 1706
Tsp45I GTSAC 5 cut(s) 806, 1000, 1698, 2029, 2155
TspDTI ATGAA 7 cut(s) 92, 188, 267, 903, 1048, 1359, 1976
TspGWI ACGGA 1 cut(s) 1797
TspRI CASTG 4 cut(s) 481, 679, 705, 802
Van91I CCANNNNNTGG 4 cut(s) 173, 537, 1595, 1820
VpaK11BI GGWCC 2 cut(s) 521, 983
VspI ATTAAT 1 cut(s) 1941
XapI RAATTY 5 cut(s) 480, 1133, 1420, 1772, 2000
XceI RCATGY 3 cut(s) 120, 786, 1647
XcmI CCANNNNNNNNNTGG 1 cut(s) 534
XmiI GTMKAC 2 cut(s) 858, 1224
XspI CTAG 6 cut(s) 824, 1200, 1611, 1758, 1806, 1920
Zsp2I ATGCAT 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.