pycom07g19290

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000390
Physical Location & Seq
Forward (+)
21644627 .. 21646299
1673 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g19290.5

Sequence Viewer

Length: 1125 bp
ATGCTGCAGACGTTCCAGATAGGGCAGCAGGTTACGATGGGGAAACTTCCAGGTATTTTATGCCATATAGGCACGTTAGACAAAAAATTGGTGGAGGGTAAAATTGTTCTTTGCGATGGGACTACCGGGGATGGGGCTATATATGCAGGCGCTTTTGGTTATGTTTTGACAAGCCGAAATGCAGCAGATGTAATCGATCCTGTTCCAGTACCAGCAGCTTCCGTTTGGTTCCATGTTGGTAACGAAATTACCCATTACATAAACTCAACCAGGAACCCAACAGCAACAATTTGGAAAAGTACTGAGGGTAGGGATGCACTGGCCCCATACGTACCTTCATTCTCATCAAGGGGTCCAAATCCAAACACTCCTAACATTCTCAAGCCAGATATCGCTTCTCCTGGAGTTTCCATTCTAGCAGCATGGCCTCCAATTACCCCAGTTTCAGGTGTTGAAGGTGATGACAGAGTAGCTTCATACAATATAATCTCGGGGACATCAATGGCATGCCCACATGCTGCGGGCGTGGCTGCATACGTCAAATCATTTCACCCCAATTGGTCACCTGCTGCTATCCTGTCAGCTCTTACGACTACTGCCAAACCTATGAGTGCAAATCTTAACCCTGAAGCCGAATTCGCATACGGTGCTGGCCTAATAAATCCTAGTAGGGCTCCGTATCCTGGTTTGGTATACGATGCTGCTGAAATCGATTACGTAAATTTTTTGTGTGCACATGGCTATAGTACCAGATTATTGAAAGCTCTTACCGGGGATAGCTGCTCATCATCACAATCTAGTCATGGAACACTCAGTGATCATCTAAACTATCCTTCTGTTGCACTTTCCACCTCGAACCCTAAATCCGTCAATGGCATTTTCAATAGGACCGTCACAAATGTTGGATCACCAAAGTCCACATATAAAGCTAAAGTGAGTGCACCACCAGGACTTGAAATCAAAGTTAATCCAAGCGTTCTAAAGTTCACATCTCTCTGGCAGAAGCTATCGTTTCAAGTCACGGTGAAAGGGTTGATTGAAAAAACCATAGTCTCTGGTTCTCTGGTGTGGGATGATGGTAATTTCCAGGTGAGGAGCCCCATTGTTGTGTATTTTGTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

375

Amino Acids

39.63

Weight (kDa)

8.47

Isoelectric Point (pI)

37.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 574
Acc36I ACCTGC 2 cut(s) 19, 574
AccI GTMKAC 1 cut(s) 693
AciI CCGC 1 cut(s) 521
AclWI GGATC 2 cut(s) 191, 913
AcsI RAATTY 2 cut(s) 635, 721
AcuI CTGAAG 1 cut(s) 648
AdeI CACNNNGTG 1 cut(s) 815
AfaI GTAC 4 cut(s) 210, 301, 333, 748
AfiI CCNNNNNNNGG 3 cut(s) 132, 446, 683
AgsI TTSAA 6 cut(s) 455, 760, 883, 956, 1016, 1040
AjnI CCWGG 6 cut(s) 49, 269, 400, 682, 946, 1086
AloI GAACNNNNNNTCC 2 cut(s) 848, 880
AluBI AGCT 7 cut(s) 218, 473, 584, 764, 780, 929, 1006
AluI AGCT 7 cut(s) 218, 473, 584, 764, 780, 929, 1006
Alw21I GWGCWC 2 cut(s) 736, 943
Alw26I GTCTC 1 cut(s) 1057
Alw44I GTGCAC 2 cut(s) 732, 939
AlwI GGATC 2 cut(s) 191, 913
Ama87I CYCGRG 1 cut(s) 490
AoxI GGCC 3 cut(s) 321, 425, 652
ApaLI GTGCAC 2 cut(s) 732, 939
ApoI RAATTY 2 cut(s) 635, 721
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 3 cut(s) 322, 353, 888
AsuC2I CCSGG 2 cut(s) 127, 772
AsuHPI GGTGA 6 cut(s) 470, 542, 555, 900, 1036, 1102
AvaI CYCGRG 1 cut(s) 490
AvaII GGWCC 2 cut(s) 353, 888
BaeGI GKGCMC 2 cut(s) 736, 943
BanII GRGCYC 2 cut(s) 676, 1100
Bbv12I GWGCWC 2 cut(s) 736, 943
BbvI GCAGC 9 cut(s) 37, 194, 227, 431, 505, 517, 556, 688, 767
BccI CCATC 4 cut(s) 31, 110, 125, 1070
BciT130I CCWGG 6 cut(s) 51, 271, 402, 684, 948, 1088
BciVI GTATCC 1 cut(s) 690
BclI TGATCA 1 cut(s) 817
BcnI CCSGG 2 cut(s) 127, 772
BcoDI GTCTC 1 cut(s) 1057
BfaI CTAG 3 cut(s) 416, 666, 798
BfmI CTRYAG 2 cut(s) 5, 742
BfoI RGCGCY 1 cut(s) 153
BfuAI ACCTGC 2 cut(s) 19, 574
BfuI GTATCC 1 cut(s) 690
BglI GCCNNNNNGGC 1 cut(s) 69
BmcAI AGTACT 1 cut(s) 301
Bme1390I CCNGG 8 cut(s) 51, 127, 271, 402, 684, 772, 948, 1088
Bme18I GGWCC 2 cut(s) 353, 888
BmeT110I CYCGRG 1 cut(s) 490
BmgT120I GGNCC 3 cut(s) 322, 353, 888
BmiI GGNNCC 6 cut(s) 230, 275, 324, 354, 675, 1097
BmrFI CCNGG 8 cut(s) 51, 127, 271, 402, 684, 772, 948, 1088
BmrI ACTGGG 1 cut(s) 434
BmsI GCATC 2 cut(s) 304, 688
BmuI ACTGGG 1 cut(s) 434
BpmI CTGGAG 1 cut(s) 423
BpuEI CTTGAG 1 cut(s) 365
BpuMI CCSGG 2 cut(s) 127, 772
Bsa29I ATCGAT 2 cut(s) 195, 711
BsaAI YACGTR 2 cut(s) 331, 718
BsaJI CCNNGG 2 cut(s) 126, 771
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 446, 683
Bse1I ACTGG 3 cut(s) 206, 324, 440
BseBI CCWGG 6 cut(s) 51, 271, 402, 684, 948, 1088
BseCI ATCGAT 2 cut(s) 195, 711
BseDI CCNNGG 2 cut(s) 126, 771
BseGI GGATG 3 cut(s) 136, 319, 1078
BseLI CCNNNNNNNGG 3 cut(s) 132, 446, 683
BseMII CTCAG 2 cut(s) 294, 826
BseNI ACTGG 3 cut(s) 206, 324, 440
BseRI GAGGAG 1 cut(s) 1108
BseSI GKGCMC 2 cut(s) 736, 943
BseXI GCAGC 9 cut(s) 37, 194, 227, 431, 505, 517, 556, 688, 767
BshFI GGCC 3 cut(s) 323, 427, 654
BshVI ATCGAT 2 cut(s) 195, 711
BsiHKAI GWGCWC 2 cut(s) 736, 943
BsiHKCI CYCGRG 1 cut(s) 490
BsiSI CCGG 2 cut(s) 126, 771
BslFI GGGAC 2 cut(s) 133, 508
BslI CCNNNNNNNGG 3 cut(s) 132, 446, 683
BsmAI GTCTC 1 cut(s) 1057
BsmFI GGGAC 2 cut(s) 133, 508
BsnI GGCC 3 cut(s) 323, 427, 654
BsoBI CYCGRG 1 cut(s) 490
Bsp1286I GDGCHC 4 cut(s) 676, 736, 943, 1100
Bsp143I GATC 3 cut(s) 196, 817, 905
BspACI CCGC 1 cut(s) 521
BspANI GGCC 3 cut(s) 323, 427, 654
BspCNI CTCAG 2 cut(s) 295, 825
BspDI ATCGAT 2 cut(s) 195, 711
BspLI GGNNCC 6 cut(s) 230, 275, 324, 354, 675, 1097
BspMAI CTGCAG 1 cut(s) 9
BspMI ACCTGC 2 cut(s) 19, 574
BspPI GGATC 2 cut(s) 191, 913
BsrI ACTGG 3 cut(s) 206, 324, 440
BssECI CCNNGG 2 cut(s) 126, 771
BssMI GATC 3 cut(s) 196, 817, 905
BssNAI GTATAC 1 cut(s) 694
Bst1107I GTATAC 1 cut(s) 694
Bst2UI CCWGG 6 cut(s) 51, 271, 402, 684, 948, 1088
Bst4CI ACNGT 3 cut(s) 647, 892, 1024
BstAPI GCANNNNNTGC 1 cut(s) 647
BstBAI YACGTR 2 cut(s) 331, 718
BstC8I GCNNGC 4 cut(s) 148, 508, 523, 652
BstDEI CTNAG 2 cut(s) 303, 812
BstEII GGTNACC 1 cut(s) 561
BstF5I GGATG 3 cut(s) 136, 319, 1078
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 3 cut(s) 199, 820, 908
BstMAI GTCTC 1 cut(s) 1057
BstMBI GATC 3 cut(s) 196, 817, 905
BstMWI GCNNNNNNNGC 5 cut(s) 69, 143, 527, 638, 647
BstNI CCWGG 6 cut(s) 51, 271, 402, 684, 948, 1088
BstNSI RCATGY 2 cut(s) 510, 518
BstPI GGTNACC 1 cut(s) 561
BstSCI CCNGG 8 cut(s) 49, 125, 269, 400, 682, 770, 946, 1086
BstSFI CTRYAG 2 cut(s) 5, 742
BstSLI GKGCMC 2 cut(s) 736, 943
BstSNI TACGTA 2 cut(s) 331, 718
BstV1I GCAGC 9 cut(s) 37, 194, 227, 431, 505, 517, 556, 688, 767
BstZ17I GTATAC 1 cut(s) 694
Bsu15I ATCGAT 2 cut(s) 195, 711
BsuI GTATCC 1 cut(s) 690
BsuRI GGCC 3 cut(s) 323, 427, 654
BsuTUI ATCGAT 2 cut(s) 195, 711
BtgZI GCGATG 1 cut(s) 129
BtsCI GGATG 3 cut(s) 136, 319, 1078
BtsIMutI CAGTG 2 cut(s) 317, 820
BveI ACCTGC 2 cut(s) 19, 574
Cac8I GCNNGC 4 cut(s) 148, 508, 523, 652
CfoI GCGC 1 cut(s) 152
Cfr13I GGNCC 3 cut(s) 322, 353, 888
ClaI ATCGAT 2 cut(s) 195, 711
Csp6I GTAC 4 cut(s) 209, 300, 332, 747
CviAII CATG 6 cut(s) 233, 423, 507, 515, 737, 803
CviQI GTAC 4 cut(s) 209, 300, 332, 747
DdeI CTNAG 2 cut(s) 303, 812
DpnI GATC 3 cut(s) 198, 819, 907
DpnII GATC 3 cut(s) 196, 817, 905
DraIII CACNNNGTG 1 cut(s) 815
Eco105I TACGTA 2 cut(s) 331, 718
Eco24I GRGCYC 2 cut(s) 676, 1100
Eco32I GATATC 1 cut(s) 391
Eco47I GGWCC 2 cut(s) 353, 888
Eco57I CTGAAG 1 cut(s) 648
Eco88I CYCGRG 1 cut(s) 490
Eco91I GGTNACC 1 cut(s) 561
EcoO65I GGTNACC 1 cut(s) 561
EcoRI GAATTC 1 cut(s) 635
EcoRII CCWGG 6 cut(s) 49, 269, 400, 682, 946, 1086
EcoRV GATATC 1 cut(s) 391
EcoT38I GRGCYC 2 cut(s) 676, 1100
FaeI CATG 6 cut(s) 236, 426, 510, 518, 740, 806
FaqI GGGAC 2 cut(s) 133, 508
FatI CATG 6 cut(s) 232, 422, 506, 514, 736, 802
FauI CCCGC 1 cut(s) 514
FbaI TGATCA 1 cut(s) 817
FblI GTMKAC 1 cut(s) 693
FokI GGATG 3 cut(s) 143, 326, 1085
FriOI GRGCYC 2 cut(s) 676, 1100
FspBI CTAG 3 cut(s) 416, 666, 798
GlaI GCGC 1 cut(s) 151
GsuI CTGGAG 1 cut(s) 423
HaeII RGCGCY 1 cut(s) 153
HaeIII GGCC 3 cut(s) 323, 427, 654
HapII CCGG 2 cut(s) 126, 771
HhaI GCGC 1 cut(s) 152
Hin1II CATG 6 cut(s) 236, 426, 510, 518, 740, 806
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HpaII CCGG 2 cut(s) 126, 771
HphI GGTGA 6 cut(s) 470, 542, 555, 900, 1036, 1102
Hpy166II GTNNAC 5 cut(s) 694, 734, 918, 941, 987
Hpy188III TCNNGA 1 cut(s) 16
Hpy8I GTNNAC 5 cut(s) 694, 734, 918, 941, 987
HpyAV CCTTC 3 cut(s) 345, 449, 843
HpyCH4III ACNGT 3 cut(s) 647, 892, 1024
HpyCH4IV ACGT 5 cut(s) 11, 74, 330, 537, 717
HpyCH4V TGCA 9 cut(s) 7, 146, 182, 317, 533, 614, 734, 842, 941
HpyF10VI GCNNNNNNNGC 5 cut(s) 69, 143, 527, 638, 647
HpyF3I CTNAG 2 cut(s) 303, 812
HpySE526I ACGT 5 cut(s) 11, 74, 330, 537, 717
Hsp92II CATG 6 cut(s) 236, 426, 510, 518, 740, 806
HspAI GCGC 1 cut(s) 150
Ksp22I TGATCA 1 cut(s) 817
Kzo9I GATC 3 cut(s) 196, 817, 905
LmnI GCTCC 2 cut(s) 679, 1095
Lsp1109I GCAGC 9 cut(s) 37, 194, 227, 431, 505, 517, 556, 688, 767
LweI GCATC 2 cut(s) 304, 688
MaeI CTAG 3 cut(s) 416, 666, 798
MaeII ACGT 5 cut(s) 11, 74, 330, 537, 717
MaeIII GTNAC 5 cut(s) 31, 239, 561, 892, 1018
MalI GATC 3 cut(s) 198, 819, 907
MboI GATC 3 cut(s) 196, 817, 905
MfeI CAATTG 1 cut(s) 556
MhlI GDGCHC 4 cut(s) 676, 736, 943, 1100
MluCI AATT 9 cut(s) 86, 102, 246, 288, 432, 556, 635, 721, 1081
MmeI TCCRAC 1 cut(s) 883
MnlI CCTC 5 cut(s) 88, 298, 438, 862, 1086
MseI TTAA 2 cut(s) 621, 966
MslI CAYNNNNRTG 1 cut(s) 1106
MspI CCGG 2 cut(s) 126, 771
MspR9I CCNGG 8 cut(s) 51, 127, 271, 402, 684, 772, 948, 1088
MunI CAATTG 1 cut(s) 556
MvaI CCWGG 6 cut(s) 51, 271, 402, 684, 948, 1088
MwoI GCNNNNNNNGC 5 cut(s) 69, 143, 527, 638, 647
NciI CCSGG 2 cut(s) 127, 772
NdeII GATC 3 cut(s) 196, 817, 905
NlaIII CATG 6 cut(s) 236, 426, 510, 518, 740, 806
NlaIV GGNNCC 6 cut(s) 230, 275, 324, 354, 675, 1097
NmuCI GTSAC 3 cut(s) 561, 892, 1018
NspI RCATGY 2 cut(s) 510, 518
PaeI GCATGC 1 cut(s) 510
PaqCI CACCTGC 1 cut(s) 574
PfoI TCCNGGA 1 cut(s) 400
Ppu21I YACGTR 2 cut(s) 331, 718
Psp6I CCWGG 6 cut(s) 49, 269, 400, 682, 946, 1086
PspEI GGTNACC 1 cut(s) 561
PspGI CCWGG 6 cut(s) 49, 269, 400, 682, 946, 1086
PspN4I GGNNCC 6 cut(s) 230, 275, 324, 354, 675, 1097
PspPI GGNCC 3 cut(s) 322, 353, 888
PstI CTGCAG 1 cut(s) 9
RsaI GTAC 4 cut(s) 210, 301, 333, 748
RsaNI GTAC 4 cut(s) 209, 300, 332, 747
RseI CAYNNNNRTG 1 cut(s) 1106
SaqAI TTAA 2 cut(s) 621, 966
Sau3AI GATC 3 cut(s) 196, 817, 905
Sau96I GGNCC 3 cut(s) 322, 353, 888
ScaI AGTACT 1 cut(s) 301
ScrFI CCNGG 8 cut(s) 51, 127, 271, 402, 684, 772, 948, 1088
SduI GDGCHC 4 cut(s) 676, 736, 943, 1100
SfaNI GCATC 2 cut(s) 304, 688
SfcI CTRYAG 2 cut(s) 5, 742
SinI GGWCC 2 cut(s) 353, 888
SmiMI CAYNNNNRTG 1 cut(s) 1106
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
SnaBI TACGTA 2 cut(s) 331, 718
SphI GCATGC 1 cut(s) 510
Sse9I AATT 9 cut(s) 86, 102, 246, 288, 432, 556, 635, 721, 1081
SsiI CCGC 1 cut(s) 521
SspMI CTAG 3 cut(s) 416, 666, 798
StyD4I CCNGG 8 cut(s) 49, 125, 269, 400, 682, 770, 946, 1086
TaaI ACNGT 3 cut(s) 647, 892, 1024
TaiI ACGT 5 cut(s) 14, 77, 333, 540, 720
TaqI TCGA 3 cut(s) 195, 711, 854
TasI AATT 9 cut(s) 86, 102, 246, 288, 432, 556, 635, 721, 1081
TatI WGTACW 1 cut(s) 299
Tru1I TTAA 2 cut(s) 621, 966
Tru9I TTAA 2 cut(s) 621, 966
TscAI CASTG 2 cut(s) 324, 820
TseFI GTSAC 3 cut(s) 561, 892, 1018
Tsp45I GTSAC 3 cut(s) 561, 892, 1018
TspDTI ATGAA 2 cut(s) 327, 465
TspGWI ACGGA 3 cut(s) 211, 666, 856
TspRI CASTG 2 cut(s) 324, 820
VneI GTGCAC 2 cut(s) 732, 939
VpaK11BI GGWCC 2 cut(s) 353, 888
XapI RAATTY 2 cut(s) 635, 721
XceI RCATGY 2 cut(s) 510, 518
XmiI GTMKAC 1 cut(s) 693
XspI CTAG 3 cut(s) 416, 666, 798
ZrmI AGTACT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.