RLG00000027255

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000390
Physical Location & Seq
Reverse (-)
10005410 .. 10008786
3377 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027255

Sequence Viewer

Length: 2247 bp
ATGGCTCTTCGATGGTTTCTGCTTCTCAGACTCATTGGCAGTATTCTACTTCTTGTTGATGCTACTCACTCAGCTGCTCGGGATGCCCGAAAGGCGTATATAGTGTATATGGGCGACAAGCCAAAGAACGGGGTTCCGGTAACACCAGATCTTCATGTGAATATTCTACGAGACGTAGTTGACAGCAACACTAATAATAATATTTTACATGAAACTCTGCTTCTTCATAGTTACAAGAGAAGTTTTCATGGATTTGCTGCAATGCTCACAGAACAAGAAGCACAAAAACTGGCTGAAATAGATGGTGTAGTGTCTGTCTTCCCAAGCAAAAAGAAGAGCGTCAAAACAACAAAGTCGTGGAACTTCCTTGGGTTTCCAGAAACAATTAAGAGAAGCCCCCTTGAAAAGGATATCATTGTTGGTGTGATCGACACTGGAATTTGGCCAGAGTCGCACAGCTTTAGTGATGTTGGATTTAGTCCTCCACCCAAGAAATGGAAAGGCATATGTCAAAGCAATGGCAACTTTACTTGTAACAATAAAATCATTGGAGCACGGTATTACCATCATTCTGGACCCTTTGAAGAAGGAGAATTTGAGTCTCCAAGAGACTCAACGGGTCATGGAACCCACACTGCATCAACAGCAGCTGGGAACATCGTGAGAGAAGCGAGCCTAAATGGTTTAGGGTTGGGAACAGCAAGAGGAGGGGTGCCATCAGCACATATTGCAGTGTACAAAGCATGTTGGGAGGATGGCTGTGATGATGCTGACGTTCTAGCAGCATTTGATGATGCTGTTTCTGACGGTGTTGACATTCTCTCTGTTTCTGCCGGGAGCAAGATTCCAGATGATTATTTGGAGGATGGATTTGCAATTGGGTCATTTCATGCTACCATAAATGGGATATTGGTTTCGATGTCCGCTGGAAACGAAGGTCCGAAAAAAAAAACTGTGGGAAACTATGCACCATGGCAACTCTCTGTGGGTGCTATCAACATAAACCGCCAATTCATCACCAAGGTTCAATTGGGTAATGGTAAAATCTATGAGGGACTTTTACCAAACTCATATGACCTCCGAGGTAAATTCTATGATTTAATTTATGGTGGAGATGCACCCAATACAAAGCCAGGTGGTAATAGGATGTCCAGATATTGTAAGAAAGATAGCTTAGAAGAAGATTTGGTCAAAGGTAAAATTGTGCTTTGTGATAGAGGCCCTGATAAGATTGAGGATGACGATTGGGATGGGTCTGGGGCCTTACAGGCTAATGCAATTGGAATTATTTTGACGGGCGTTAAAGTTGCTGAGGAAATTGTTGACCCTATTCCTCGTCCTGCATCTCACGTCGGATTGGAAGAAAATAGCAATATTTACATGTACATAAACAAAACAAGGAACCCAACTGCAACTATTTGGAAAAGTCAAGAGATTAATGATGTATTGGCTCCATATGTGCCCTCCTACTCATCAAGGGGTCCAAATCCGGTGAATCCTAACATTCTTAAGCCAGATTTAGTGGCTCCAGGAACTGAGATTCTAGCAGCATATGATCAACCAATTGGTGATGATGCTAACAATAGAGCTGAATACAATTTGGATACTGGGACATCAATGGCGTGCCCCCATGCTACGGCCGTAGCTGCATACGTCAAATCATTTCACCCTGAATGGTCACCGGCTGCTATCAAATCAGCTCTCATGACTACCGCTACACCCATGAGTGCCAAAACTAGCCCGCACGCTGAATTTGCATATGGAGTTGGCCTAATAAACCCTTCTAGAGCTCCATATCCTGGTTTGGTATACGATCTTGATGTAAAAGACTATGTAGATTTTTTGTGTTCACAAGGATATAATGACAAACTATTGGAACAAATAATCGGGGTCAAGGCTACCTCATGCTCGTCAAAATCTAATAATGAAACAGCCAATGACCTGAACTATCCTTCTTTTACTCTTTCCATCAAGGATCCAGAATTCGTCAATGGGGTCTTCCAAAGGACTGTGACTAATGTTGGATCATCGAATTCCACATATAGAGCTAAAATAGTGGCTCAATCGGGACTCAAAATCAATGTGGATCCGAGTGTGCTGTCGTTCACATCTCTTGGGCAGAAGAAATCTTTTGTTGTCACAGTAAAAGGGCCGATTGAGAAATCAAATATAGTCTCTGCGTCTCTAATATGGGATGATGGTGCTTTCCAAGTTAGGAGCCCCATCGTTGTCTATGTTGCAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

749

Amino Acids

80.94

Weight (kDa)

5.93

Isoelectric Point (pI)

38.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 33 - 111 4e-14 Peptidase inhibitor I9
Peptidase_S8 PF00082 136 - 590 3.1e-44 Subtilase family
fn3_6 PF17766 647 - 744 1.4e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 712
AccB7I CCANNNNNTGG 2 cut(s) 495, 1799
AccI GTMKAC 1 cut(s) 1809
AciI CCGC 4 cut(s) 924, 1006, 1713, 1742
AclWI GGATC 5 cut(s) 1970, 1983, 2032, 2081, 2094
AcoI YGGCCR 2 cut(s) 443, 1638
AcsI RAATTY 6 cut(s) 438, 593, 1088, 1751, 1982, 2032
AfaI GTAC 2 cut(s) 737, 1385
AfiI CCNNNNNNNGG 6 cut(s) 128, 406, 495, 903, 1636, 1799
AflII CTTAAG 1 cut(s) 1508
AflIII ACRYGT 1 cut(s) 1380
AgsI TTSAA 3 cut(s) 404, 584, 1028
AjiI CACGTC 1 cut(s) 1351
AjnI CCWGG 3 cut(s) 1132, 1528, 1798
AluBI AGCT 9 cut(s) 74, 459, 650, 1173, 1589, 1646, 1700, 1790, 2048
AluI AGCT 9 cut(s) 74, 459, 650, 1173, 1589, 1646, 1700, 1790, 2048
Alw21I GWGCWC 2 cut(s) 556, 1792
Alw26I GTCTC 5 cut(s) 165, 603, 606, 2179, 2187
AlwI GGATC 5 cut(s) 1970, 1983, 2032, 2081, 2094
AlwNI CAGNNNCTG 2 cut(s) 650, 1535
Ama87I CYCGRG 1 cut(s) 78
AoxI GGCC 6 cut(s) 443, 1219, 1260, 1638, 1768, 2150
ApeKI GCWGC 7 cut(s) 74, 257, 647, 782, 1547, 1646, 1685
ApoI RAATTY 6 cut(s) 438, 593, 1088, 1751, 1982, 2032
AseI ATTAAT 1 cut(s) 1437
AspS9I GGNCC 6 cut(s) 575, 938, 1220, 1260, 1481, 2150
AsuC2I CCSGG 1 cut(s) 835
AsuHPI GGTGA 5 cut(s) 1009, 1504, 1580, 1658, 1671
AvaI CYCGRG 1 cut(s) 78
AvaII GGWCC 3 cut(s) 575, 938, 1481
BaeGI GKGCMC 2 cut(s) 1464, 1628
BalI TGGCCA 1 cut(s) 445
BamHI GGATCC 2 cut(s) 1975, 2086
BanI GGYRCC 1 cut(s) 712
BanII GRGCYC 2 cut(s) 1792, 2222
BbsI GAAGAC 2 cut(s) 310, 1990
Bbv12I GWGCWC 2 cut(s) 556, 1792
BbvCI CCTCAGC 1 cut(s) 1311
BbvI GCAGC 7 cut(s) 61, 244, 659, 794, 1559, 1633, 1672
BceAI ACGGC 2 cut(s) 1625, 1653
BciT130I CCWGG 3 cut(s) 1134, 1530, 1800
BciVI GTATCC 1 cut(s) 1597
BclI TGATCA 1 cut(s) 1555
BcnI CCSGG 1 cut(s) 835
BcoDI GTCTC 5 cut(s) 165, 603, 606, 2179, 2187
BfaI CTAG 4 cut(s) 779, 1544, 1737, 1785
BfrI CTTAAG 1 cut(s) 1508
BfuI GTATCC 1 cut(s) 1597
BglI GCCNNNNNGGC 2 cut(s) 92, 1268
BglII AGATCT 1 cut(s) 148
BisI GCNGC 7 cut(s) 75, 258, 648, 783, 1548, 1647, 1686
BlsI GCNGC 7 cut(s) 76, 259, 649, 784, 1549, 1648, 1687
Bme1390I CCNGG 4 cut(s) 835, 1134, 1530, 1800
Bme18I GGWCC 3 cut(s) 575, 938, 1481
BmeT110I CYCGRG 1 cut(s) 78
BmgBI CACGTC 1 cut(s) 1351
BmgT120I GGNCC 6 cut(s) 575, 938, 1220, 1260, 1481, 2150
BmrFI CCNGG 4 cut(s) 835, 1134, 1530, 1800
BmrI ACTGGG 1 cut(s) 1617
BmsI GCATC 8 cut(s) 49, 73, 647, 757, 784, 1105, 1352, 1564
BmuI ACTGGG 1 cut(s) 1617
BpiI GAAGAC 2 cut(s) 310, 1990
BpmI CTGGAG 1 cut(s) 1512
Bpu10I CCTNAGC 1 cut(s) 1311
BpuMI CCSGG 1 cut(s) 835
BsaJI CCNNGG 4 cut(s) 367, 971, 1020, 1081
BsaWI WCCGGW 2 cut(s) 136, 1489
Bsc4I CCNNNNNNNGG 6 cut(s) 128, 406, 495, 903, 1636, 1799
Bse118I RCCGGY 1 cut(s) 1681
Bse1I ACTGG 3 cut(s) 294, 439, 1612
Bse3DI GCAATG 2 cut(s) 267, 523
BseBI CCWGG 3 cut(s) 1134, 1530, 1800
BseDI CCNNGG 4 cut(s) 367, 971, 1020, 1081
BseGI GGATG 7 cut(s) 88, 760, 871, 1152, 1243, 1255, 2200
BseLI CCNNNNNNNGG 6 cut(s) 128, 406, 495, 903, 1636, 1799
BseMI GCAATG 2 cut(s) 267, 523
BseMII CTCAG 4 cut(s) 40, 84, 1302, 1527
BseNI ACTGG 3 cut(s) 294, 439, 1612
BseRI GAGGAG 1 cut(s) 720
BseSI GKGCMC 2 cut(s) 1464, 1628
BseX3I CGGCCG 1 cut(s) 1638
BseXI GCAGC 7 cut(s) 61, 244, 659, 794, 1559, 1633, 1672
BseYI CCCAGC 1 cut(s) 650
Bsh1285I CGRYCG 1 cut(s) 1641
BshFI GGCC 6 cut(s) 445, 1221, 1262, 1640, 1770, 2152
BshNI GGYRCC 1 cut(s) 712
BsiEI CGRYCG 1 cut(s) 1641
BsiHKAI GWGCWC 2 cut(s) 556, 1792
BsiHKCI CYCGRG 1 cut(s) 78
BsiSI CCGG 4 cut(s) 137, 834, 1490, 1682
BslFI GGGAC 3 cut(s) 1068, 1624, 2082
BslI CCNNNNNNNGG 6 cut(s) 128, 406, 495, 903, 1636, 1799
BsmAI GTCTC 5 cut(s) 165, 603, 606, 2179, 2187
BsmBI CGTCTC 2 cut(s) 165, 2187
BsmFI GGGAC 3 cut(s) 1068, 1624, 2082
BsnI GGCC 6 cut(s) 445, 1221, 1262, 1640, 1770, 2152
BsoBI CYCGRG 1 cut(s) 78
Bsp1286I GDGCHC 5 cut(s) 556, 1464, 1628, 1792, 2222
Bsp1407I TGTACA 2 cut(s) 735, 1383
Bsp143I GATC 7 cut(s) 148, 426, 1555, 1813, 1975, 2024, 2086
Bsp19I CCATGG 1 cut(s) 971
BspACI CCGC 4 cut(s) 924, 1006, 1713, 1742
BspANI GGCC 6 cut(s) 445, 1221, 1262, 1640, 1770, 2152
BspCNI CTCAG 4 cut(s) 39, 83, 1303, 1528
BspHI TCATGA 1 cut(s) 1704
BspPI GGATC 5 cut(s) 1970, 1983, 2032, 2081, 2094
BspQI GCTCTTC 2 cut(s) 12, 329
BspT107I GGYRCC 1 cut(s) 712
BspTI CTTAAG 1 cut(s) 1508
BsrDI GCAATG 2 cut(s) 267, 523
BsrFI RCCGGY 1 cut(s) 1681
BsrGI TGTACA 2 cut(s) 735, 1383
BsrI ACTGG 3 cut(s) 294, 439, 1612
BssAI RCCGGY 1 cut(s) 1681
BssECI CCNNGG 4 cut(s) 367, 971, 1020, 1081
BssMI GATC 7 cut(s) 148, 426, 1555, 1813, 1975, 2024, 2086
BssNAI GTATAC 1 cut(s) 1810
BssT1I CCWWGG 3 cut(s) 367, 971, 1020
Bst1107I GTATAC 1 cut(s) 1810
Bst2UI CCWGG 3 cut(s) 1134, 1530, 1800
Bst4CI ACNGT 5 cut(s) 558, 809, 955, 2011, 2143
Bst6I CTCTTC 2 cut(s) 12, 329
BstAFI CTTAAG 1 cut(s) 1508
BstAPI GCANNNNNTGC 1 cut(s) 728
BstAUI TGTACA 2 cut(s) 735, 1383
BstC8I GCNNGC 4 cut(s) 673, 1624, 1742, 1746
BstDEI CTNAG 5 cut(s) 26, 70, 1174, 1311, 1536
BstDSI CCRYGG 1 cut(s) 971
BstEII GGTNACC 1 cut(s) 1677
BstENI CCTNNNNNAGG 1 cut(s) 404
BstF5I GGATG 7 cut(s) 88, 760, 871, 1152, 1243, 1255, 2200
BstKTI GATC 7 cut(s) 151, 429, 1558, 1816, 1978, 2027, 2089
BstMAI GTCTC 5 cut(s) 165, 603, 606, 2179, 2187
BstMBI GATC 7 cut(s) 148, 426, 1555, 1813, 1975, 2024, 2086
BstMCI CGRYCG 1 cut(s) 1641
BstMWI GCNNNNNNNGC 8 cut(s) 83, 92, 451, 644, 728, 1268, 1646, 1754
BstNI CCWGG 3 cut(s) 1134, 1530, 1800
BstNSI RCATGY 2 cut(s) 747, 1384
BstPI GGTNACC 1 cut(s) 1677
BstSCI CCNGG 4 cut(s) 833, 1132, 1528, 1798
BstSLI GKGCMC 2 cut(s) 1464, 1628
BstV1I GCAGC 7 cut(s) 61, 244, 659, 794, 1559, 1633, 1672
BstV2I GAAGAC 2 cut(s) 310, 1990
BstX2I RGATCY 3 cut(s) 148, 1975, 2086
BstXI CCANNNNNNTGG 1 cut(s) 572
BstYI RGATCY 3 cut(s) 148, 1975, 2086
BstZ17I GTATAC 1 cut(s) 1810
BstZI CGGCCG 1 cut(s) 1638
BsuI GTATCC 1 cut(s) 1597
BsuRI GGCC 6 cut(s) 445, 1221, 1262, 1640, 1770, 2152
BtgI CCRYGG 1 cut(s) 971
BtrI CACGTC 1 cut(s) 1351
BtsCI GGATG 7 cut(s) 88, 760, 871, 1152, 1243, 1255, 2200
BtsI GCAGTG 2 cut(s) 633, 738
BtsIMutI CAGTG 3 cut(s) 432, 633, 738
Cac8I GCNNGC 4 cut(s) 673, 1624, 1742, 1746
CaiI CAGNNNCTG 2 cut(s) 650, 1535
CciI TCATGA 1 cut(s) 1704
Cfr10I RCCGGY 1 cut(s) 1681
Cfr13I GGNCC 6 cut(s) 575, 938, 1220, 1260, 1481, 2150
CseI GACGC 2 cut(s) 328, 2169
Csp6I GTAC 2 cut(s) 736, 1384
CviQI GTAC 2 cut(s) 736, 1384
DdeI CTNAG 5 cut(s) 26, 70, 1174, 1311, 1536
DpnI GATC 7 cut(s) 150, 428, 1557, 1815, 1977, 2026, 2088
DpnII GATC 7 cut(s) 148, 426, 1555, 1813, 1975, 2024, 2086
EaeI YGGCCR 2 cut(s) 443, 1638
EagI CGGCCG 1 cut(s) 1638
Eam1104I CTCTTC 2 cut(s) 12, 329
EarI CTCTTC 2 cut(s) 12, 329
Ecl136II GAGCTC 1 cut(s) 1790
EclXI CGGCCG 1 cut(s) 1638
Eco130I CCWWGG 3 cut(s) 367, 971, 1020
Eco24I GRGCYC 2 cut(s) 1792, 2222
Eco32I GATATC 1 cut(s) 412
Eco47I GGWCC 3 cut(s) 575, 938, 1481
Eco52I CGGCCG 1 cut(s) 1638
Eco53kI GAGCTC 1 cut(s) 1790
Eco88I CYCGRG 1 cut(s) 78
Eco91I GGTNACC 1 cut(s) 1677
EcoICRI GAGCTC 1 cut(s) 1790
EcoNI CCTNNNNNAGG 1 cut(s) 404
EcoO109I RGGNCCY 2 cut(s) 1220, 1260
EcoO65I GGTNACC 1 cut(s) 1677
EcoRI GAATTC 2 cut(s) 1982, 2032
EcoRII CCWGG 3 cut(s) 1132, 1528, 1798
EcoRV GATATC 1 cut(s) 412
EcoT14I CCWWGG 3 cut(s) 367, 971, 1020
EcoT38I GRGCYC 2 cut(s) 1792, 2222
ErhI CCWWGG 3 cut(s) 367, 971, 1020
Esp3I CGTCTC 2 cut(s) 165, 2187
FaqI GGGAC 3 cut(s) 1068, 1624, 2082
FauI CCCGC 1 cut(s) 1749
FauNDI CATATG 5 cut(s) 506, 1072, 1456, 1552, 1759
FbaI TGATCA 1 cut(s) 1555
FblI GTMKAC 1 cut(s) 1809
Fnu4HI GCNGC 7 cut(s) 75, 258, 648, 783, 1548, 1647, 1686
FokI GGATG 7 cut(s) 95, 767, 878, 1159, 1250, 1262, 2207
FriOI GRGCYC 2 cut(s) 1792, 2222
Fsp4HI GCNGC 7 cut(s) 75, 258, 648, 783, 1548, 1647, 1686
FspBI CTAG 4 cut(s) 779, 1544, 1737, 1785
GluI GCNGC 7 cut(s) 75, 258, 648, 783, 1548, 1647, 1686
GsaI CCCAGC 1 cut(s) 654
GsuI CTGGAG 1 cut(s) 1512
HaeIII GGCC 6 cut(s) 445, 1221, 1262, 1640, 1770, 2152
HapII CCGG 4 cut(s) 137, 834, 1490, 1682
HgaI GACGC 2 cut(s) 328, 2169
HincII GTYRAC 3 cut(s) 181, 814, 1324
HindII GTYRAC 3 cut(s) 181, 814, 1324
HinfI GANTC 8 cut(s) 30, 449, 599, 611, 844, 1495, 1540, 2070
HpaII CCGG 4 cut(s) 137, 834, 1490, 1682
HphI GGTGA 5 cut(s) 1009, 1504, 1580, 1658, 1671
Hpy166II GTNNAC 7 cut(s) 181, 736, 814, 1324, 1810, 1850, 2106
Hpy188I TCNGA 6 cut(s) 29, 805, 942, 1082, 1355, 2091
Hpy8I GTNNAC 7 cut(s) 181, 736, 814, 1324, 1810, 1850, 2106
Hpy99I CGWCG 1 cut(s) 1355
HpyAV CCTTC 4 cut(s) 581, 929, 1791, 1962
HpyCH4III ACNGT 5 cut(s) 558, 809, 955, 2011, 2143
HpyCH4IV ACGT 4 cut(s) 174, 774, 1350, 1653
HpyF10VI GCNNNNNNNGC 8 cut(s) 83, 92, 451, 644, 728, 1268, 1646, 1754
HpyF3I CTNAG 5 cut(s) 26, 70, 1174, 1311, 1536
HpySE526I ACGT 4 cut(s) 174, 774, 1350, 1653
Ksp22I TGATCA 1 cut(s) 1555
Kzo9I GATC 7 cut(s) 148, 426, 1555, 1813, 1975, 2024, 2086
LguI GCTCTTC 2 cut(s) 12, 329
LmnI GCTCC 6 cut(s) 551, 837, 1456, 1531, 1795, 2217
Lsp1109I GCAGC 7 cut(s) 61, 244, 659, 794, 1559, 1633, 1672
LweI GCATC 8 cut(s) 49, 73, 647, 757, 784, 1105, 1352, 1564
MaeI CTAG 4 cut(s) 779, 1544, 1737, 1785
MaeII ACGT 4 cut(s) 174, 774, 1350, 1653
MaeIII GTNAC 6 cut(s) 139, 230, 533, 1677, 2011, 2137
MalI GATC 7 cut(s) 150, 428, 1557, 1815, 1977, 2026, 2088
MboI GATC 7 cut(s) 148, 426, 1555, 1813, 1975, 2024, 2086
MfeI CAATTG 4 cut(s) 876, 1028, 1278, 1563
MflI RGATCY 3 cut(s) 148, 1975, 2086
MhlI GDGCHC 5 cut(s) 556, 1464, 1628, 1792, 2222
MlsI TGGCCA 1 cut(s) 445
MluNI TGGCCA 1 cut(s) 445
MlyI GAGTC 5 cut(s) 24, 458, 605, 608, 2064
MmeI TCCRAC 3 cut(s) 451, 1333, 2002
Mox20I TGGCCA 1 cut(s) 445
MscI TGGCCA 1 cut(s) 445
MseI TTAA 5 cut(s) 387, 1100, 1302, 1437, 1509
Msp20I TGGCCA 1 cut(s) 445
MspA1I CMGCKG 3 cut(s) 74, 650, 926
MspCI CTTAAG 1 cut(s) 1508
MspI CCGG 4 cut(s) 137, 834, 1490, 1682
MspR9I CCNGG 4 cut(s) 835, 1134, 1530, 1800
MunI CAATTG 4 cut(s) 876, 1028, 1278, 1563
MvaI CCWGG 3 cut(s) 1134, 1530, 1800
MwoI GCNNNNNNNGC 8 cut(s) 83, 92, 451, 644, 728, 1268, 1646, 1754
NciI CCSGG 1 cut(s) 835
NcoI CCATGG 1 cut(s) 971
NdeI CATATG 5 cut(s) 506, 1072, 1456, 1552, 1759
NdeII GATC 7 cut(s) 148, 426, 1555, 1813, 1975, 2024, 2086
NmuCI GTSAC 3 cut(s) 1677, 2011, 2137
NspI RCATGY 2 cut(s) 747, 1384
PagI TCATGA 1 cut(s) 1704
PciI ACATGT 1 cut(s) 1380
PciSI GCTCTTC 2 cut(s) 12, 329
PcsI WCGNNNNNNNCGW 1 cut(s) 85
PfeI GAWTC 3 cut(s) 844, 1495, 1540
PflMI CCANNNNNTGG 2 cut(s) 495, 1799
PfoI TCCNGGA 1 cut(s) 1528
PkrI GCNGC 7 cut(s) 76, 259, 649, 784, 1549, 1648, 1687
PleI GAGTC 5 cut(s) 24, 457, 605, 607, 2064
PpsI GAGTC 5 cut(s) 24, 457, 605, 607, 2064
PscI ACATGT 1 cut(s) 1380
PshBI ATTAAT 1 cut(s) 1437
Psp124BI GAGCTC 1 cut(s) 1792
Psp6I CCWGG 3 cut(s) 1132, 1528, 1798
PspEI GGTNACC 1 cut(s) 1677
PspFI CCCAGC 1 cut(s) 650
PspGI CCWGG 3 cut(s) 1132, 1528, 1798
PspPI GGNCC 6 cut(s) 575, 938, 1220, 1260, 1481, 2150
PstNI CAGNNNCTG 2 cut(s) 650, 1535
PsuI RGATCY 3 cut(s) 148, 1975, 2086
PvuII CAGCTG 2 cut(s) 74, 650
RsaI GTAC 2 cut(s) 737, 1385
RsaNI GTAC 2 cut(s) 736, 1384
SacI GAGCTC 1 cut(s) 1792
SapI GCTCTTC 2 cut(s) 12, 329
SaqAI TTAA 5 cut(s) 387, 1100, 1302, 1437, 1509
SatI GCNGC 7 cut(s) 75, 258, 648, 783, 1548, 1647, 1686
Sau3AI GATC 7 cut(s) 148, 426, 1555, 1813, 1975, 2024, 2086
Sau96I GGNCC 6 cut(s) 575, 938, 1220, 1260, 1481, 2150
SchI GAGTC 5 cut(s) 24, 458, 605, 608, 2064
ScrFI CCNGG 4 cut(s) 835, 1134, 1530, 1800
SduI GDGCHC 5 cut(s) 556, 1464, 1628, 1792, 2222
SfaNI GCATC 8 cut(s) 49, 73, 647, 757, 784, 1105, 1352, 1564
SinI GGWCC 3 cut(s) 575, 938, 1481
SmlI CTYRAG 1 cut(s) 1508
SmoI CTYRAG 1 cut(s) 1508
SsiI CCGC 4 cut(s) 924, 1006, 1713, 1742
SspI AATATT 3 cut(s) 163, 202, 1375
SspMI CTAG 4 cut(s) 779, 1544, 1737, 1785
SstI GAGCTC 1 cut(s) 1792
StyD4I CCNGG 4 cut(s) 833, 1132, 1528, 1798
StyI CCWWGG 3 cut(s) 367, 971, 1020
TaaI ACNGT 5 cut(s) 558, 809, 955, 2011, 2143
TaiI ACGT 4 cut(s) 177, 777, 1353, 1656
TaqI TCGA 4 cut(s) 10, 429, 917, 2030
TatI WGTACW 2 cut(s) 735, 1383
TfiI GAWTC 3 cut(s) 844, 1495, 1540
Tru1I TTAA 5 cut(s) 387, 1100, 1302, 1437, 1509
Tru9I TTAA 5 cut(s) 387, 1100, 1302, 1437, 1509
TscAI CASTG 3 cut(s) 439, 640, 738
TseFI GTSAC 3 cut(s) 1677, 2011, 2137
TseI GCWGC 7 cut(s) 74, 257, 647, 782, 1547, 1646, 1685
Tsp45I GTSAC 3 cut(s) 1677, 2011, 2137
TspDTI ATGAA 7 cut(s) 143, 215, 225, 236, 878, 1003, 1941
TspRI CASTG 3 cut(s) 439, 640, 738
Van91I CCANNNNNTGG 2 cut(s) 495, 1799
Vha464I CTTAAG 1 cut(s) 1508
VpaK11BI GGWCC 3 cut(s) 575, 938, 1481
VspI ATTAAT 1 cut(s) 1437
XagI CCTNNNNNAGG 1 cut(s) 404
XapI RAATTY 6 cut(s) 438, 593, 1088, 1751, 1982, 2032
XbaI TCTAGA 1 cut(s) 1784
XceI RCATGY 2 cut(s) 747, 1384
XcmI CCANNNNNNNNNTGG 2 cut(s) 492, 1027
XmiI GTMKAC 1 cut(s) 1809
XspI CTAG 4 cut(s) 779, 1544, 1737, 1785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.