pycom07g19260

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000390
Physical Location & Seq
Reverse (-)
21589664 .. 21593239
3576 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g19260.6

Sequence Viewer

Length: 2229 bp
ATGGCTTTGACTCCATGGTTTCTCCTTCTCATCAGCCTCATATCTATTCTGCTAGTTGATGTCACTCTATCTGCTGCTCACCAGGATAACCGGAAGGATTATATTGTGTATATGGGTGACAAGCCAAAGCCGGAGCTTTCCACCACTACCACATCAGCTCTTCATGTAAACATGCTACAAAACGTCGTCGTCGACAGCAATATTGCGCACGAATCTCTGCTTCTGCACAGCTACAAGAGAAGCTTCAATGGCTTTGCTGCCAGGCTAACAGAGGAAGAAGCACAGAAGTTGGCTGGAATGGATGGTGTGGTGTCTGTTTTCCCTAGTGAAACTAAGAAGCCCCAAACAACAAGGTCATGGGACTTCATTGGGTTTCCTGAAATGGTGAAGAGAAGCGCCATTGAAACTGATATCATTGTCGGTCTGATCGACTCTGGAATTTGGCCTGAATCTGCCAGCTTCAGTGACGCCGGGTTTGGCCCACCCCCAAAAAGGTGGAAGGGCGCATGCAAGGGCGAAGGCAATTTTACTTGCAACAATAAAATTATTGGAGCACGGTATTACCGCAGTCTACCCTACCCCAAAAATAGCAGTGATATCCTGTCCCCGAGAGACACGGAAGGCCATGGAACCCACTGTGCATCAACAGCAGCAGGGAACTTAGTTAGCAAGGCAAGTCTGTATGGTTTAGGGTTGGGGACAGCAAGAGGAGGGGTGCCATCAGCACGCATTGCGGTGTACAAAGTTTGTTGGTCAGACGGGTGCCCGGATGCTGATATTCTAGCGGCATTCGATGATGCCATAGCTGACGGTGTTGATATACTCTCTGTCTCCCTTGGGGGGCTTAAACCGTTAGATTATTTCAGAAATTCCATTGACATTGGAGCTTTTCACGCTTTAAGAAAAGGAATATTCACTTCCGCGGCTGCTGGTAATGAAGGTCCAAACCTGAAAACTATTACAAACTTTGCACCATGGTCTCTTGCTGTAGCTGCTAGCACCATAGATCGCCACTTTGACACCAAGGTTCAATTGGGAAACCACAAAATTTATGAGGGAATAGTAACAAACACATTTGAACTAAAGGGTAAATTCTACCCCATAGTATATGCTGCAGACGTTCCAGATCGGGCAGCAGGTTACGATGGGGAAACTTCCAAGTTATGCCATATAGGCACGTTAGACAAAAAATTGGTGGAGGGTAAAATTGTTCTTTGCGATGGGACTACTGGGGATGGGGCTATATATGCAGGCGCATTTGGTTACGTTTTGACAAGCCGAATTGCAGCAGATGTAATCGATCCTGTTTCAGTACCAGCAGCTTCTGTTTGGTTCCATGTTGGTAACGAAATTACCCATTACATAAACTCAACCAGGAGCCCAACAGCAACAATTTGGAAAAGTACTGAGGGTAGGGATGCACTGGCCCCATATATACCTTCATTCTCATCAAGGGGTCCAAATCCAAATACTCCTAACATTCTGAAGCCAGATATCGCTTCTCCTGGAGTTTCCATTCTAGCAGCATGGCCTCCAATTGCCCCAGTTTCAGGTGTTGAAGGTGATGACAGAGTAGCTTCATACAATATAATCTCGGGGACATCAATGGCATGCCCACATGCTGCGGGCGTGGCTGCATATGTCAAATCATTTCACCCCAATTGGTCACCTGCTGCTATCCTGTCAGCTCTTACGACTACTGCCAAATCTATGAGTGCAAATCTTAACCCTGAAGCCGAATTCGCATACGGTGCTGGCCTAATAAATCCTAGTAGGGCTCCGTATCCTGGTTTGGTATACGATGCTGGTGAAATCGATTACGTAAATTTTTTGTGTGCACATGGCTATAGTACCAGATTATTGAAAGCTCTTACCGGGGATAGCTGCTCATCATCACAATCTAGTCATGGAACACTCAGTGATCATCTAAACTATCCTTCTGTTGCACTTTCAACCTCGAACCCTAAATCCGTCAATGGCATTTTCAATAGGACCGTCACAAATGTTGGATCACCAAAGTCCACATATAAAGCTAAAGTGAGTGCACCACCAGGACTTGAAATCAAAGTTAATCCAAGCGTTCTAAAGTTCACATCTCTCGGGCAGAAGCTATCGTTTCAAGTCACGGTGAAAGGGTTGATTGAAAAAACCGTAGTCTCAGGTTCTCTGGTGTGGGATGATGGTAATTTCCAAGTGAGGAGCCCCATAGTTGTGTATTTTGTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

743

Amino Acids

79.11

Weight (kDa)

6.98

Isoelectric Point (pI)

33.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1678
Acc16I TGCGCA 1 cut(s) 207
Acc36I ACCTGC 2 cut(s) 1127, 1678
AccB1I GGYRCC 2 cut(s) 715, 762
AccI GTMKAC 3 cut(s) 192, 571, 1797
AccII CGCG 1 cut(s) 923
AciI CCGC 6 cut(s) 565, 734, 785, 921, 923, 1625
AclWI GGATC 2 cut(s) 1295, 2017
AcsI RAATTY 6 cut(s) 438, 868, 1047, 1091, 1739, 1825
AcuI CTGAAG 3 cut(s) 445, 1505, 1752
AcyI GRCGYC 1 cut(s) 468
AdeI CACNNNGTG 1 cut(s) 1919
AfaI GTAC 4 cut(s) 740, 1314, 1405, 1852
AfiI CCNNNNNNNGG 4 cut(s) 492, 840, 1550, 1787
AjnI CCWGG 6 cut(s) 81, 260, 1373, 1504, 1786, 2050
AloI GAACNNNNNNTCC 2 cut(s) 1952, 1984
Alw21I GWGCWC 3 cut(s) 556, 1840, 2047
Alw26I GTCTC 4 cut(s) 606, 835, 984, 2161
Alw44I GTGCAC 2 cut(s) 1836, 2043
AlwI GGATC 2 cut(s) 1295, 2017
AlwNI CAGNNNCTG 1 cut(s) 1325
Ama87I CYCGRG 3 cut(s) 607, 1594, 2099
AoxI GGCC 6 cut(s) 443, 478, 622, 1425, 1529, 1756
ApaLI GTGCAC 2 cut(s) 1836, 2043
ApoI RAATTY 6 cut(s) 438, 868, 1047, 1091, 1739, 1825
ArsI GACNNNNNNTTYG 2 cut(s) 458, 490
AspLEI GCGC 4 cut(s) 208, 398, 506, 1256
AspS9I GGNCC 5 cut(s) 479, 941, 1426, 1457, 1992
AsuC2I CCSGG 3 cut(s) 472, 767, 1876
AsuHPI GGTGA 9 cut(s) 71, 128, 397, 1574, 1646, 1659, 1820, 2004, 2140
AsuNHI GCTAGC 1 cut(s) 995
AvaI CYCGRG 3 cut(s) 607, 1594, 2099
AvaII GGWCC 3 cut(s) 941, 1457, 1992
BaeGI GKGCMC 3 cut(s) 767, 1840, 2047
BanI GGYRCC 2 cut(s) 715, 762
BanII GRGCYC 3 cut(s) 1382, 1780, 2204
Bbv12I GWGCWC 3 cut(s) 556, 1840, 2047
BccI CCATC 6 cut(s) 296, 727, 1139, 1214, 1229, 2174
BciT130I CCWGG 6 cut(s) 83, 262, 1375, 1506, 1788, 2052
BciVI GTATCC 1 cut(s) 1794
BclI TGATCA 1 cut(s) 1921
BcnI CCSGG 3 cut(s) 472, 767, 1876
BcoDI GTCTC 4 cut(s) 606, 835, 984, 2161
BfaI CTAG 7 cut(s) 53, 324, 782, 996, 1520, 1770, 1902
BfmI CTRYAG 3 cut(s) 987, 1113, 1846
BfoI RGCGCY 1 cut(s) 399
BfuAI ACCTGC 2 cut(s) 1127, 1678
BfuI GTATCC 1 cut(s) 1794
BglI GCCNNNNNGGC 1 cut(s) 1173
BmcAI AGTACT 1 cut(s) 1405
Bme1390I CCNGG 9 cut(s) 83, 262, 472, 767, 1375, 1506, 1788, 1876, 2052
Bme18I GGWCC 3 cut(s) 941, 1457, 1992
BmeT110I CYCGRG 3 cut(s) 607, 1594, 2099
BmgT120I GGNCC 5 cut(s) 479, 941, 1426, 1457, 1992
BmiI GGNNCC 9 cut(s) 631, 717, 764, 1334, 1379, 1428, 1458, 1779, 2201
BmrFI CCNGG 9 cut(s) 83, 262, 472, 767, 1375, 1506, 1788, 1876, 2052
BmrI ACTGGG 2 cut(s) 1239, 1538
BmsI GCATC 5 cut(s) 650, 760, 787, 1408, 1792
BmtI GCTAGC 1 cut(s) 999
BmuI ACTGGG 2 cut(s) 1239, 1538
BpmI CTGGAG 1 cut(s) 1527
BpuMI CCSGG 3 cut(s) 472, 767, 1876
Bsa29I ATCGAT 2 cut(s) 1299, 1815
BsaAI YACGTR 1 cut(s) 1822
BsaHI GRCGYC 1 cut(s) 468
BsaI GGTCTC 1 cut(s) 984
BsaJI CCNNGG 7 cut(s) 14, 625, 835, 921, 974, 1023, 1875
BsaWI WCCGGW 1 cut(s) 90
Bsc4I CCNNNNNNNGG 4 cut(s) 492, 840, 1550, 1787
Bse1I ACTGG 3 cut(s) 1234, 1428, 1544
Bse3DI GCAATG 1 cut(s) 729
BseBI CCWGG 6 cut(s) 83, 262, 1375, 1506, 1788, 2052
BseCI ATCGAT 2 cut(s) 1299, 1815
BseDI CCNNGG 7 cut(s) 14, 625, 835, 921, 974, 1023, 1875
BseGI GGATG 5 cut(s) 307, 775, 1240, 1423, 2182
BseLI CCNNNNNNNGG 4 cut(s) 492, 840, 1550, 1787
BseMI GCAATG 1 cut(s) 729
BseMII CTCAG 3 cut(s) 1398, 1930, 2172
BseNI ACTGG 3 cut(s) 1234, 1428, 1544
BseRI GAGGAG 2 cut(s) 723, 2212
BseSI GKGCMC 3 cut(s) 767, 1840, 2047
BsgI GTGCAG 1 cut(s) 209
Bsh1236I CGCG 1 cut(s) 923
BshFI GGCC 6 cut(s) 445, 480, 624, 1427, 1531, 1758
BshNI GGYRCC 2 cut(s) 715, 762
BshVI ATCGAT 2 cut(s) 1299, 1815
BsiHKAI GWGCWC 3 cut(s) 556, 1840, 2047
BsiHKCI CYCGRG 3 cut(s) 607, 1594, 2099
BsiSI CCGG 5 cut(s) 91, 131, 471, 767, 1875
BslFI GGGAC 5 cut(s) 374, 589, 712, 1237, 1612
BslI CCNNNNNNNGG 4 cut(s) 492, 840, 1550, 1787
BsmAI GTCTC 4 cut(s) 606, 835, 984, 2161
BsmFI GGGAC 5 cut(s) 374, 589, 712, 1237, 1612
BsmI GAATGC 1 cut(s) 788
BsnI GGCC 6 cut(s) 445, 480, 624, 1427, 1531, 1758
Bso31I GGTCTC 1 cut(s) 984
BsoBI CYCGRG 3 cut(s) 607, 1594, 2099
Bsp1286I GDGCHC 7 cut(s) 556, 767, 1382, 1780, 1840, 2047, 2204
Bsp1407I TGTACA 1 cut(s) 738
Bsp143I GATC 6 cut(s) 426, 1006, 1126, 1300, 1921, 2009
Bsp19I CCATGG 3 cut(s) 14, 625, 974
BspACI CCGC 6 cut(s) 565, 734, 785, 921, 923, 1625
BspANI GGCC 6 cut(s) 445, 480, 624, 1427, 1531, 1758
BspCNI CTCAG 3 cut(s) 1399, 1929, 2171
BspDI ATCGAT 2 cut(s) 1299, 1815
BspFNI CGCG 1 cut(s) 923
BspLI GGNNCC 9 cut(s) 631, 717, 764, 1334, 1379, 1428, 1458, 1779, 2201
BspMAI CTGCAG 1 cut(s) 1117
BspMI ACCTGC 2 cut(s) 1127, 1678
BspOI GCTAGC 1 cut(s) 999
BspPI GGATC 2 cut(s) 1295, 2017
BspQI GCTCTTC 1 cut(s) 165
BspT107I GGYRCC 2 cut(s) 715, 762
BspTNI GGTCTC 1 cut(s) 984
BsrDI GCAATG 1 cut(s) 729
BsrGI TGTACA 1 cut(s) 738
BsrI ACTGG 3 cut(s) 1234, 1428, 1544
BssECI CCNNGG 7 cut(s) 14, 625, 835, 921, 974, 1023, 1875
BssMI GATC 6 cut(s) 426, 1006, 1126, 1300, 1921, 2009
BssNAI GTATAC 1 cut(s) 1798
BssNI GRCGYC 1 cut(s) 468
BssT1I CCWWGG 5 cut(s) 14, 625, 835, 974, 1023
Bst1107I GTATAC 1 cut(s) 1798
Bst2UI CCWGG 6 cut(s) 83, 262, 1375, 1506, 1788, 2052
Bst4CI ACNGT 8 cut(s) 558, 638, 812, 852, 1751, 1996, 2128, 2152
Bst6I CTCTTC 2 cut(s) 165, 383
BstACI GRCGYC 1 cut(s) 468
BstAPI GCANNNNNTGC 2 cut(s) 731, 1751
BstAUI TGTACA 1 cut(s) 738
BstBAI YACGTR 1 cut(s) 1822
BstC8I GCNNGC 8 cut(s) 457, 508, 727, 997, 1252, 1612, 1627, 1756
BstDEI CTNAG 5 cut(s) 333, 661, 1407, 1916, 2158
BstDSI CCRYGG 4 cut(s) 14, 625, 921, 974
BstEII GGTNACC 1 cut(s) 1665
BstF5I GGATG 5 cut(s) 307, 775, 1240, 1423, 2182
BstFNI CGCG 1 cut(s) 923
BstH2I RGCGCY 1 cut(s) 399
BstHHI GCGC 4 cut(s) 208, 398, 506, 1256
BstKTI GATC 6 cut(s) 429, 1009, 1129, 1303, 1924, 2012
BstMAI GTCTC 4 cut(s) 606, 835, 984, 2161
BstMBI GATC 6 cut(s) 426, 1006, 1126, 1300, 1921, 2009
BstNI CCWGG 6 cut(s) 83, 262, 1375, 1506, 1788, 2052
BstNSI RCATGY 4 cut(s) 175, 510, 1614, 1622
BstPI GGTNACC 1 cut(s) 1665
BstSCI CCNGG 9 cut(s) 81, 260, 470, 765, 1373, 1504, 1786, 1874, 2050
BstSFI CTRYAG 3 cut(s) 987, 1113, 1846
BstSLI GKGCMC 3 cut(s) 767, 1840, 2047
BstSNI TACGTA 1 cut(s) 1822
BstUI CGCG 1 cut(s) 923
BstXI CCANNNNNNTGG 1 cut(s) 495
BstZ17I GTATAC 1 cut(s) 1798
Bsu15I ATCGAT 2 cut(s) 1299, 1815
BsuI GTATCC 1 cut(s) 1794
BsuRI GGCC 6 cut(s) 445, 480, 624, 1427, 1531, 1758
BsuTUI ATCGAT 2 cut(s) 1299, 1815
BtgI CCRYGG 4 cut(s) 14, 625, 921, 974
BtgZI GCGATG 1 cut(s) 1233
BtsCI GGATG 5 cut(s) 307, 775, 1240, 1423, 2182
BtsI GCAGTG 1 cut(s) 598
BtsIMutI CAGTG 5 cut(s) 469, 598, 634, 1421, 1924
BveI ACCTGC 2 cut(s) 1127, 1678
Cac8I GCNNGC 8 cut(s) 457, 508, 727, 997, 1252, 1612, 1627, 1756
CaiI CAGNNNCTG 1 cut(s) 1325
CfoI GCGC 4 cut(s) 208, 398, 506, 1256
Cfr13I GGNCC 5 cut(s) 479, 941, 1426, 1457, 1992
Cfr42I CCGCGG 1 cut(s) 924
ClaI ATCGAT 2 cut(s) 1299, 1815
CseI GACGC 1 cut(s) 476
Csp6I GTAC 4 cut(s) 739, 1313, 1404, 1851
CviQI GTAC 4 cut(s) 739, 1313, 1404, 1851
DdeI CTNAG 5 cut(s) 333, 661, 1407, 1916, 2158
DpnI GATC 6 cut(s) 428, 1008, 1128, 1302, 1923, 2011
DpnII GATC 6 cut(s) 426, 1006, 1126, 1300, 1921, 2009
DraIII CACNNNGTG 1 cut(s) 1919
Eam1104I CTCTTC 2 cut(s) 165, 383
EarI CTCTTC 2 cut(s) 165, 383
Eco105I TACGTA 1 cut(s) 1822
Eco130I CCWWGG 5 cut(s) 14, 625, 835, 974, 1023
Eco24I GRGCYC 3 cut(s) 1382, 1780, 2204
Eco31I GGTCTC 1 cut(s) 984
Eco32I GATATC 3 cut(s) 412, 598, 1495
Eco47I GGWCC 3 cut(s) 941, 1457, 1992
Eco57I CTGAAG 3 cut(s) 445, 1505, 1752
Eco88I CYCGRG 3 cut(s) 607, 1594, 2099
Eco91I GGTNACC 1 cut(s) 1665
EcoO65I GGTNACC 1 cut(s) 1665
EcoRI GAATTC 1 cut(s) 1739
EcoRII CCWGG 6 cut(s) 81, 260, 1373, 1504, 1786, 2050
EcoRV GATATC 3 cut(s) 412, 598, 1495
EcoT14I CCWWGG 5 cut(s) 14, 625, 835, 974, 1023
EcoT38I GRGCYC 3 cut(s) 1382, 1780, 2204
ErhI CCWWGG 5 cut(s) 14, 625, 835, 974, 1023
FalI AAGNNNNNCTT 2 cut(s) 227, 259
FaqI GGGAC 5 cut(s) 374, 589, 712, 1237, 1612
FauI CCCGC 1 cut(s) 1618
FauNDI CATATG 1 cut(s) 1639
FbaI TGATCA 1 cut(s) 1921
FblI GTMKAC 3 cut(s) 192, 571, 1797
FokI GGATG 5 cut(s) 314, 782, 1247, 1430, 2189
FriOI GRGCYC 3 cut(s) 1382, 1780, 2204
FspBI CTAG 7 cut(s) 53, 324, 782, 996, 1520, 1770, 1902
FspI TGCGCA 1 cut(s) 207
GlaI GCGC 4 cut(s) 207, 397, 505, 1255
GsuI CTGGAG 1 cut(s) 1527
HaeII RGCGCY 1 cut(s) 399
HaeIII GGCC 6 cut(s) 445, 480, 624, 1427, 1531, 1758
HapII CCGG 5 cut(s) 91, 131, 471, 767, 1875
HgaI GACGC 1 cut(s) 476
HhaI GCGC 4 cut(s) 208, 398, 506, 1256
Hin1I GRCGYC 1 cut(s) 468
Hin6I GCGC 4 cut(s) 206, 396, 504, 1254
HinP1I GCGC 4 cut(s) 206, 396, 504, 1254
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HindIII AAGCTT 1 cut(s) 241
HinfI GANTC 4 cut(s) 10, 212, 431, 449
HpaII CCGG 5 cut(s) 91, 131, 471, 767, 1875
HphI GGTGA 9 cut(s) 71, 128, 397, 1574, 1646, 1659, 1820, 2004, 2140
Hpy166II GTNNAC 9 cut(s) 169, 193, 572, 739, 1798, 1838, 2022, 2045, 2091
Hpy188I TCNGA 4 cut(s) 426, 757, 866, 1485
Hpy188III TCNNGA 3 cut(s) 377, 435, 1124
Hpy8I GTNNAC 9 cut(s) 169, 193, 572, 739, 1798, 1838, 2022, 2045, 2091
Hpy99I CGWCG 3 cut(s) 188, 191, 194
HpyAV CCTTC 9 cut(s) 35, 88, 493, 512, 614, 932, 1449, 1553, 1947
HpyCH4III ACNGT 8 cut(s) 558, 638, 812, 852, 1751, 1996, 2128, 2152
HpyCH4IV ACGT 5 cut(s) 183, 1119, 1178, 1266, 1821
HpyF3I CTNAG 5 cut(s) 333, 661, 1407, 1916, 2158
HpySE526I ACGT 5 cut(s) 183, 1119, 1178, 1266, 1821
Hsp92I GRCGYC 1 cut(s) 468
HspAI GCGC 4 cut(s) 206, 396, 504, 1254
Ksp22I TGATCA 1 cut(s) 1921
KspI CCGCGG 1 cut(s) 924
Kzo9I GATC 6 cut(s) 426, 1006, 1126, 1300, 1921, 2009
LguI GCTCTTC 1 cut(s) 165
LmnI GCTCC 6 cut(s) 133, 551, 884, 1377, 1783, 2199
LweI GCATC 5 cut(s) 650, 760, 787, 1408, 1792
MaeI CTAG 7 cut(s) 53, 324, 782, 996, 1520, 1770, 1902
MaeII ACGT 5 cut(s) 183, 1119, 1178, 1266, 1821
MalI GATC 6 cut(s) 428, 1008, 1128, 1302, 1923, 2011
MboI GATC 6 cut(s) 426, 1006, 1126, 1300, 1921, 2009
MboII GAAGA 3 cut(s) 152, 287, 400
MfeI CAATTG 3 cut(s) 1031, 1536, 1660
MhlI GDGCHC 7 cut(s) 556, 767, 1382, 1780, 1840, 2047, 2204
MlyI GAGTC 2 cut(s) 4, 425
MmeI TCCRAC 1 cut(s) 1987
MseI TTAA 4 cut(s) 846, 899, 1725, 2070
MslI CAYNNNNRTG 2 cut(s) 734, 2210
MspA1I CMGCKG 1 cut(s) 923
MspI CCGG 5 cut(s) 91, 131, 471, 767, 1875
MspR9I CCNGG 9 cut(s) 83, 262, 472, 767, 1375, 1506, 1788, 1876, 2052
MunI CAATTG 3 cut(s) 1031, 1536, 1660
Mva1269I GAATGC 1 cut(s) 788
MvaI CCWGG 6 cut(s) 83, 262, 1375, 1506, 1788, 2052
MvnI CGCG 1 cut(s) 923
NciI CCSGG 3 cut(s) 472, 767, 1876
NcoI CCATGG 3 cut(s) 14, 625, 974
NdeI CATATG 1 cut(s) 1639
NdeII GATC 6 cut(s) 426, 1006, 1126, 1300, 1921, 2009
NheI GCTAGC 1 cut(s) 995
NlaIV GGNNCC 9 cut(s) 631, 717, 764, 1334, 1379, 1428, 1458, 1779, 2201
NmuCI GTSAC 6 cut(s) 61, 116, 464, 1665, 1996, 2122
NsbI TGCGCA 1 cut(s) 207
NspI RCATGY 4 cut(s) 175, 510, 1614, 1622
PaeI GCATGC 2 cut(s) 510, 1614
PaqCI CACCTGC 1 cut(s) 1678
PciSI GCTCTTC 1 cut(s) 165
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 426
PctI GAATGC 1 cut(s) 788
PfeI GAWTC 2 cut(s) 212, 449
PfoI TCCNGGA 1 cut(s) 1504
PleI GAGTC 2 cut(s) 4, 425
PpsI GAGTC 2 cut(s) 4, 425
Ppu21I YACGTR 1 cut(s) 1822
Psp6I CCWGG 6 cut(s) 81, 260, 1373, 1504, 1786, 2050
PspEI GGTNACC 1 cut(s) 1665
PspGI CCWGG 6 cut(s) 81, 260, 1373, 1504, 1786, 2050
PspN4I GGNNCC 9 cut(s) 631, 717, 764, 1334, 1379, 1428, 1458, 1779, 2201
PspPI GGNCC 5 cut(s) 479, 941, 1426, 1457, 1992
PstI CTGCAG 1 cut(s) 1117
PstNI CAGNNNCTG 1 cut(s) 1325
RsaI GTAC 4 cut(s) 740, 1314, 1405, 1852
RsaNI GTAC 4 cut(s) 739, 1313, 1404, 1851
RseI CAYNNNNRTG 2 cut(s) 734, 2210
SacII CCGCGG 1 cut(s) 924
SalI GTCGAC 1 cut(s) 191
SapI GCTCTTC 1 cut(s) 165
SaqAI TTAA 4 cut(s) 846, 899, 1725, 2070
Sau3AI GATC 6 cut(s) 426, 1006, 1126, 1300, 1921, 2009
Sau96I GGNCC 5 cut(s) 479, 941, 1426, 1457, 1992
ScaI AGTACT 1 cut(s) 1405
SchI GAGTC 2 cut(s) 4, 425
ScrFI CCNGG 9 cut(s) 83, 262, 472, 767, 1375, 1506, 1788, 1876, 2052
SduI GDGCHC 7 cut(s) 556, 767, 1382, 1780, 1840, 2047, 2204
SfaNI GCATC 5 cut(s) 650, 760, 787, 1408, 1792
SfcI CTRYAG 3 cut(s) 987, 1113, 1846
Sfr303I CCGCGG 1 cut(s) 924
SgrBI CCGCGG 1 cut(s) 924
SinI GGWCC 3 cut(s) 941, 1457, 1992
SmiMI CAYNNNNRTG 2 cut(s) 734, 2210
SnaBI TACGTA 1 cut(s) 1822
SphI GCATGC 2 cut(s) 510, 1614
SsiI CCGC 6 cut(s) 565, 734, 785, 921, 923, 1625
SspI AATATT 2 cut(s) 202, 912
SspMI CTAG 7 cut(s) 53, 324, 782, 996, 1520, 1770, 1902
StyD4I CCNGG 9 cut(s) 81, 260, 470, 765, 1373, 1504, 1786, 1874, 2050
StyI CCWWGG 5 cut(s) 14, 625, 835, 974, 1023
TaaI ACNGT 8 cut(s) 558, 638, 812, 852, 1751, 1996, 2128, 2152
TaiI ACGT 5 cut(s) 186, 1122, 1181, 1269, 1824
TaqI TCGA 6 cut(s) 192, 429, 792, 1299, 1815, 1958
TaqII GACCGA 1 cut(s) 410
TatI WGTACW 2 cut(s) 738, 1403
TauI GCSGC 2 cut(s) 788, 926
TfiI GAWTC 2 cut(s) 212, 449
Tru1I TTAA 4 cut(s) 846, 899, 1725, 2070
Tru9I TTAA 4 cut(s) 846, 899, 1725, 2070
TscAI CASTG 5 cut(s) 469, 598, 641, 1428, 1924
TseFI GTSAC 6 cut(s) 61, 116, 464, 1665, 1996, 2122
Tsp45I GTSAC 6 cut(s) 61, 116, 464, 1665, 1996, 2122
TspDTI ATGAA 5 cut(s) 152, 355, 951, 1431, 1569
TspGWI ACGGA 3 cut(s) 632, 1770, 1960
TspRI CASTG 5 cut(s) 469, 598, 641, 1428, 1924
VneI GTGCAC 2 cut(s) 1836, 2043
VpaK11BI GGWCC 3 cut(s) 941, 1457, 1992
XapI RAATTY 6 cut(s) 438, 868, 1047, 1091, 1739, 1825
XceI RCATGY 4 cut(s) 175, 510, 1614, 1622
XcmI CCANNNNNNNNNTGG 1 cut(s) 1030
XmiI GTMKAC 3 cut(s) 192, 571, 1797
XspI CTAG 7 cut(s) 53, 324, 782, 996, 1520, 1770, 1902
ZrmI AGTACT 1 cut(s) 1405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.