Rh1CG339600

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0000390
Physical Location & Seq
Reverse (-)
61353689 .. 61361093
7405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG339600.1

Sequence Viewer

Length: 1116 bp
ATGGCCTCCAGGGTCAATTTTACAAAAGCAGGTGTTGACGGGAGTATCTCTAGGTTTTGTGGAAGAGATGACTTAGACGAAGATTTGATCAAAGGTAAAATTGTGCTGTGTGATGGCCCTATGGATGAGGCTGGGGTTGGGGCCATATTGGGTGGCGCAGCCGGAATTGTTCTGACAGGAAAAAAAGTCGACGATGAACTACTTGGCCCTTATCCGGTGCCTGCATCTTTCGTCGGGTTGGAAGAAAACAGCAACATTTACAAATACATAAATACAACAAGGAATGCAACTGCAACTATATGGAAAAGTGAAGAGATTAGTGATGTATTGGCACCATACGTGCCATCCTACTCATCGAGGGGCCCAAATCCGATCAATCCCAACATTCTCAAGCCAGATTTAGCGGCTCCAGGAACTTACATTCTGGCAGCATATCCCCCAAGTGGTGGTGGCAGTAGAGACGGATACTATATAGACACTGGGACATCAATGGCATGCCCTCATGCTACGGCCACAGCTGCATATGTCAAATCATTTCACCCTAAATGGTCACCGGCTGCTATCCAATCGGCTCTCATCACTACTGCTAAACCTATGAATGCCACAACTAGCCCTGACGCTGAATTTGCATACGGAGCTGGCCTAATAAACCCTTCTAGGGCGCCATATCCTGGTTTGGTGTATGATCTTGATGAACAAGACTACATAGATTTTTTGTGTTCACAAGGATACAGTGGTAAACTATTGCAAGCCATAACCAAGGACAAAACTAGTTGCTCATCAAAATCTATTAATAGAACAGCTAATGACATGAACTATCCTTCTTTTGCACTTTCTATCAAGGATTCGAAATTTGTCAATGGGGTCTTCCATAGGACTGTCACTAATGTTGGATCATCAAAATCCACATACAGAGCTAAAACGGTGGCTCCATTGGGACTCAAAATCAATGTGAATCCAAGTGTGTTATCGTTCACATCTCTCGGGCAAAAGAAATCTTTTGTTGTCACTATAAAAGGGCCGATTGAGAAATCAAATCTAGTCTCTGCGTCTTTGGTGTGGGATGATGGTGCTTTCCAAGTCAGGAGCCCCATCGTTGTCTATGTTGCTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

371

Amino Acids

39.44

Weight (kDa)

8.11

Isoelectric Point (pI)

32.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 111 - 214 3.2e-17 Subtilase family
fn3_6 PF17766 270 - 367 8e-28 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000390)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18050 FvH4_7g18050
malus_domestica MD07G1206200.v1.1 MD07G1206400.v1.1 MD07G1206500.v1.1 MD07G1206700.v1.1 MD07G1207000.v1.1 MD07G1207100.v1.1 MD07G1207200.v1.1
prunus_persica Prupe.2G166200_v2.0.a1 Prupe.2G242000_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242100_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242200_v2.0.a1 Prupe.2G242400_v2.0.a1
pyrus_communis pycom07g19260 pycom07g19280 pycom07g19290
rosa_chinensis RchiOBHm_Chr1g0325901 RchiOBHm_Chr1g0325961 RchiOBHm_Chr1g0344281 RchiOBHm_Chr1g0344291 RchiOBHm_Chr1g0344301 RchiOBHm_Chr1g0368771 RchiOBHm_Chr1g0369141 RchiOBHm_Chr1g0369281 RchiOBHm_Chr1g0369291 RchiOBHm_Chr1g0369661 RchiOBHm_Chr1g0370161 RchiOBHm_Chr4g0396401
rosa_laevigata RLG00000009546 RLG00000027070 RLG00000027140 RLG00000027151 RLG00000027152 RLG00000027190 RLG00000027255 RLG00000028909 RLG00000030094 RLG00000030097
rosa_multiflora Rmu_co8196240.1_g000001 Rmu_co8379125.1_g000001 Rmu_co8402573.1_g000001 Rmu_co8479243.1_g000001 Rmu_sc0000019.1_g000019 Rmu_sc0001154.1_g000009 Rmu_sc0001482.1_g000014 Rmu_sc0004250.1_g000009 Rmu_sc0006696.1_g000002 Rmu_sc0006696.1_g000004 Rmu_sc0008679.1_g000018 Rmu_sc0009743.1_g000003 Rmu_sc0013657.1_g000023
rosa_roxburghii Rroxscaffold_4G00286760 Rroxscaffold_4G00287250 Rroxscaffold_4G00287470 Rroxscaffold_4G00287770 Rroxscaffold_4G00287930 Rroxscaffold_4G00309920 Rroxscaffold_4G00324120
rosa_rugosa Rorug01G0055900 Rorug01G0173100 Rorug01G0173200 Rorug01G0337700 Rorug01G0337800 Rorug01G0337800 Rorug01G0337800 Rorug01G0344900 Rorug01G0349400 Rorug01G0349400 Rorug01G0351700 Rorug01G0354500
rosa_samantha Rh1AG072300 Rh1AG189000 Rh1AG189100 Rh1AG351900 Rh1AG356500 Rh1AG359800 Rh1AG363200 Rh1BG059100 Rh1BG155800 Rh1BG306800 Rh1BG315600 Rh1BG319000 Rh1BG320300 Rh1BG326300 Rh1BG326400 Rh1CG071600 Rh1CG174600 Rh1CG174800 Rh1CG322400 Rh1CG330300 Rh1CG334200 Rh1CG339600
rosa_wichuraiana Rw1G005870 Rw1G005900 Rw1G015560 Rw1G031350 Rw1G031470 Rw1G031700 Rw1G031960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 20
Acc36I ACCTGC 1 cut(s) 20
AccB1I GGYRCC 3 cut(s) 217, 331, 661
AccB7I CCANNNNNTGG 2 cut(s) 446, 671
AccI GTMKAC 1 cut(s) 189
AciI CCGC 1 cut(s) 404
AclWI GGATC 1 cut(s) 901
AcoI YGGCCR 1 cut(s) 510
AcsI RAATTY 2 cut(s) 623, 851
AcyI GRCGYC 1 cut(s) 662
AfiI CCNNNNNNNGG 5 cut(s) 214, 443, 446, 658, 671
AhlI ACTAGT 1 cut(s) 770
AjnI CCWGG 3 cut(s) 8, 409, 670
AluBI AGCT 4 cut(s) 518, 638, 803, 917
AluI AGCT 4 cut(s) 518, 638, 803, 917
Alw26I GTCTC 2 cut(s) 453, 1048
AlwI GGATC 1 cut(s) 901
Ama87I CYCGRG 1 cut(s) 983
AoxI GGCC 8 cut(s) 3, 115, 141, 205, 361, 510, 640, 1019
ApaI GGGCCC 1 cut(s) 365
ApeKI GCWGC 4 cut(s) 158, 428, 518, 557
ApoI RAATTY 2 cut(s) 623, 851
ArsI GACNNNNNNTTYG 2 cut(s) 608, 640
AseI ATTAAT 1 cut(s) 792
AspLEI GCGC 2 cut(s) 158, 664
AspS9I GGNCC 6 cut(s) 116, 141, 206, 361, 362, 1019
AsuHPI GGTGA 2 cut(s) 530, 543
AsuII TTCGAA 1 cut(s) 848
AvaI CYCGRG 1 cut(s) 983
BaeGI GKGCMC 1 cut(s) 365
BaeI ACNNNNGTAYC 4 cut(s) 28, 61, 721, 754
BanI GGYRCC 3 cut(s) 217, 331, 661
BanII GRGCYC 2 cut(s) 365, 1091
BbsI GAAGAC 1 cut(s) 859
BbvI GCAGC 4 cut(s) 170, 440, 505, 544
BccI CCATC 4 cut(s) 107, 352, 1061, 1100
BceAI ACGGC 1 cut(s) 525
BciT130I CCWGG 3 cut(s) 10, 411, 672
BciVI GTATCC 2 cut(s) 458, 722
BclI TGATCA 1 cut(s) 87
BcoDI GTCTC 2 cut(s) 453, 1048
BcuI ACTAGT 1 cut(s) 770
BfaI CTAG 5 cut(s) 51, 609, 657, 771, 1040
BfoI RGCGCY 1 cut(s) 665
BfuAI ACCTGC 1 cut(s) 20
BfuI GTATCC 2 cut(s) 458, 722
BisI GCNGC 5 cut(s) 159, 405, 429, 519, 558
BlsI GCNGC 5 cut(s) 160, 406, 430, 520, 559
Bme1390I CCNGG 3 cut(s) 10, 411, 672
BmeT110I CYCGRG 1 cut(s) 983
BmgT120I GGNCC 6 cut(s) 116, 141, 206, 361, 362, 1019
BmiI GGNNCC 9 cut(s) 142, 219, 333, 362, 363, 408, 663, 930, 1088
BmrFI CCNGG 3 cut(s) 10, 411, 672
BmrI ACTGGG 1 cut(s) 489
BmsI GCATC 1 cut(s) 233
BmuI ACTGGG 1 cut(s) 489
BpiI GAAGAC 1 cut(s) 859
BpmI CTGGAG 1 cut(s) 393
Bpu14I TTCGAA 1 cut(s) 848
BpuEI CTTGAG 1 cut(s) 374
BsaAI YACGTR 1 cut(s) 340
BsaHI GRCGYC 1 cut(s) 662
BsaJI CCNNGG 2 cut(s) 9, 759
BsaWI WCCGGW 1 cut(s) 214
BsaXI ACNNNNNCTCC 2 cut(s) 913, 943
Bsc4I CCNNNNNNNGG 5 cut(s) 214, 443, 446, 658, 671
Bse118I RCCGGY 1 cut(s) 553
Bse1I ACTGG 1 cut(s) 484
BseBI CCWGG 3 cut(s) 10, 411, 672
BseDI CCNNGG 2 cut(s) 9, 759
BseGI GGATG 3 cut(s) 130, 344, 1069
BseLI CCNNNNNNNGG 5 cut(s) 214, 443, 446, 658, 671
BseNI ACTGG 1 cut(s) 484
BseSI GKGCMC 1 cut(s) 365
BseXI GCAGC 4 cut(s) 170, 440, 505, 544
BseYI CCCAGC 1 cut(s) 131
BshFI GGCC 8 cut(s) 5, 117, 143, 207, 363, 512, 642, 1021
BshNI GGYRCC 3 cut(s) 217, 331, 661
BsiHKCI CYCGRG 1 cut(s) 983
BsiSI CCGG 3 cut(s) 162, 215, 554
BslFI GGGAC 2 cut(s) 496, 951
BslI CCNNNNNNNGG 5 cut(s) 214, 443, 446, 658, 671
BsmAI GTCTC 2 cut(s) 453, 1048
BsmBI CGTCTC 1 cut(s) 453
BsmFI GGGAC 2 cut(s) 496, 951
BsmI GAATGC 2 cut(s) 289, 604
BsnI GGCC 8 cut(s) 5, 117, 143, 207, 363, 512, 642, 1021
BsoBI CYCGRG 1 cut(s) 983
Bsp119I TTCGAA 1 cut(s) 848
Bsp120I GGGCCC 1 cut(s) 361
Bsp1286I GDGCHC 2 cut(s) 365, 1091
Bsp143I GATC 4 cut(s) 87, 372, 685, 893
BspACI CCGC 1 cut(s) 404
BspANI GGCC 8 cut(s) 5, 117, 143, 207, 363, 512, 642, 1021
BspLI GGNNCC 9 cut(s) 142, 219, 333, 362, 363, 408, 663, 930, 1088
BspMI ACCTGC 1 cut(s) 20
BspPI GGATC 1 cut(s) 901
BspT104I TTCGAA 1 cut(s) 848
BspT107I GGYRCC 3 cut(s) 217, 331, 661
BsrFI RCCGGY 1 cut(s) 553
BsrI ACTGG 1 cut(s) 484
BssAI RCCGGY 1 cut(s) 553
BssECI CCNNGG 2 cut(s) 9, 759
BssMI GATC 4 cut(s) 87, 372, 685, 893
BssNI GRCGYC 1 cut(s) 662
BssT1I CCWWGG 1 cut(s) 759
Bst2UI CCWGG 3 cut(s) 10, 411, 672
Bst4CI ACNGT 3 cut(s) 734, 880, 925
Bst6I CTCTTC 2 cut(s) 58, 306
BstACI GRCGYC 1 cut(s) 662
BstBAI YACGTR 1 cut(s) 340
BstBI TTCGAA 1 cut(s) 848
BstC8I GCNNGC 4 cut(s) 222, 496, 640, 750
BstDEI CTNAG 1 cut(s) 73
BstEII GGTNACC 1 cut(s) 549
BstF5I GGATG 3 cut(s) 130, 344, 1069
BstH2I RGCGCY 1 cut(s) 665
BstHHI GCGC 2 cut(s) 158, 664
BstKTI GATC 4 cut(s) 90, 375, 688, 896
BstMAI GTCTC 2 cut(s) 453, 1048
BstMBI GATC 4 cut(s) 87, 372, 685, 893
BstMWI GCNNNNNNNGC 3 cut(s) 518, 626, 635
BstNI CCWGG 3 cut(s) 10, 411, 672
BstNSI RCATGY 1 cut(s) 498
BstPI GGTNACC 1 cut(s) 549
BstSCI CCNGG 3 cut(s) 8, 409, 670
BstSLI GKGCMC 1 cut(s) 365
BstV1I GCAGC 4 cut(s) 170, 440, 505, 544
BstV2I GAAGAC 1 cut(s) 859
BsuI GTATCC 2 cut(s) 458, 722
BsuRI GGCC 8 cut(s) 5, 117, 143, 207, 363, 512, 642, 1021
BtsCI GGATG 3 cut(s) 130, 344, 1069
BtsIMutI CAGTG 2 cut(s) 477, 739
BveI ACCTGC 1 cut(s) 20
Cac8I GCNNGC 4 cut(s) 222, 496, 640, 750
CfoI GCGC 2 cut(s) 158, 664
Cfr10I RCCGGY 1 cut(s) 553
Cfr13I GGNCC 6 cut(s) 116, 141, 206, 361, 362, 1019
CseI GACGC 2 cut(s) 626, 1038
CviAII CATG 3 cut(s) 495, 503, 811
DdeI CTNAG 1 cut(s) 73
DinI GGCGCC 1 cut(s) 663
DpnI GATC 4 cut(s) 89, 374, 687, 895
DpnII GATC 4 cut(s) 87, 372, 685, 893
EaeI YGGCCR 1 cut(s) 510
Eam1104I CTCTTC 2 cut(s) 58, 306
EarI CTCTTC 2 cut(s) 58, 306
Eco130I CCWWGG 1 cut(s) 759
Eco24I GRGCYC 2 cut(s) 365, 1091
Eco88I CYCGRG 1 cut(s) 983
Eco91I GGTNACC 1 cut(s) 549
EcoO109I RGGNCCY 1 cut(s) 361
EcoO65I GGTNACC 1 cut(s) 549
EcoRII CCWGG 3 cut(s) 8, 409, 670
EcoT14I CCWWGG 1 cut(s) 759
EcoT38I GRGCYC 2 cut(s) 365, 1091
EgeI GGCGCC 1 cut(s) 663
EheI GGCGCC 1 cut(s) 663
ErhI CCWWGG 1 cut(s) 759
Esp3I CGTCTC 1 cut(s) 453
FaeI CATG 3 cut(s) 498, 506, 814
FaqI GGGAC 2 cut(s) 496, 951
FatI CATG 3 cut(s) 494, 502, 810
FauNDI CATATG 1 cut(s) 523
FbaI TGATCA 1 cut(s) 87
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 5 cut(s) 159, 405, 429, 519, 558
FokI GGATG 3 cut(s) 137, 331, 1076
FriOI GRGCYC 2 cut(s) 365, 1091
Fsp4HI GCNGC 5 cut(s) 159, 405, 429, 519, 558
FspBI CTAG 5 cut(s) 51, 609, 657, 771, 1040
GlaI GCGC 2 cut(s) 157, 663
GluI GCNGC 5 cut(s) 159, 405, 429, 519, 558
GsaI CCCAGC 1 cut(s) 135
GsuI CTGGAG 1 cut(s) 393
HaeII RGCGCY 1 cut(s) 665
HaeIII GGCC 8 cut(s) 5, 117, 143, 207, 363, 512, 642, 1021
HapII CCGG 3 cut(s) 162, 215, 554
HgaI GACGC 2 cut(s) 626, 1038
HhaI GCGC 2 cut(s) 158, 664
Hin1I GRCGYC 1 cut(s) 662
Hin1II CATG 3 cut(s) 498, 506, 814
Hin6I GCGC 2 cut(s) 156, 662
HinP1I GCGC 2 cut(s) 156, 662
HincII GTYRAC 2 cut(s) 37, 190
HindII GTYRAC 2 cut(s) 37, 190
HinfI GANTC 3 cut(s) 845, 939, 955
HpaII CCGG 3 cut(s) 162, 215, 554
HphI GGTGA 2 cut(s) 530, 543
Hpy166II GTNNAC 5 cut(s) 37, 190, 722, 740, 975
Hpy188I TCNGA 2 cut(s) 174, 372
Hpy188III TCNNGA 2 cut(s) 689, 1084
Hpy8I GTNNAC 5 cut(s) 37, 190, 722, 740, 975
Hpy99I CGWCG 2 cut(s) 194, 236
HpyAV CCTTC 2 cut(s) 663, 831
HpyCH4III ACNGT 3 cut(s) 734, 880, 925
HpyCH4IV ACGT 1 cut(s) 339
HpyCH4V TGCA 7 cut(s) 224, 287, 293, 521, 629, 748, 830
HpyF10VI GCNNNNNNNGC 3 cut(s) 518, 626, 635
HpyF3I CTNAG 1 cut(s) 73
HpySE526I ACGT 1 cut(s) 339
Hsp92I GRCGYC 1 cut(s) 662
Hsp92II CATG 3 cut(s) 498, 506, 814
HspAI GCGC 2 cut(s) 156, 662
KasI GGCGCC 1 cut(s) 661
Ksp22I TGATCA 1 cut(s) 87
Kzo9I GATC 4 cut(s) 87, 372, 685, 893
LmnI GCTCC 4 cut(s) 412, 635, 934, 1086
Lsp1109I GCAGC 4 cut(s) 170, 440, 505, 544
LweI GCATC 1 cut(s) 233
MaeI CTAG 5 cut(s) 51, 609, 657, 771, 1040
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 3 cut(s) 549, 880, 1006
MalI GATC 4 cut(s) 89, 374, 687, 895
MboI GATC 4 cut(s) 87, 372, 685, 893
MboII GAAGA 5 cut(s) 75, 92, 254, 323, 859
MhlI GDGCHC 2 cut(s) 365, 1091
MluCI AATT 5 cut(s) 16, 99, 165, 623, 851
Mly113I GGCGCC 1 cut(s) 662
MlyI GAGTC 1 cut(s) 933
MmeI TCCRAC 2 cut(s) 219, 871
MnlI CCTC 4 cut(s) 16, 121, 351, 510
MseI TTAA 2 cut(s) 792, 1114
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 3 cut(s) 162, 215, 554
MspR9I CCNGG 3 cut(s) 10, 411, 672
Mva1269I GAATGC 2 cut(s) 289, 604
MvaI CCWGG 3 cut(s) 10, 411, 672
MwoI GCNNNNNNNGC 3 cut(s) 518, 626, 635
NarI GGCGCC 1 cut(s) 662
NdeI CATATG 1 cut(s) 523
NdeII GATC 4 cut(s) 87, 372, 685, 893
NlaIII CATG 3 cut(s) 498, 506, 814
NlaIV GGNNCC 9 cut(s) 142, 219, 333, 362, 363, 408, 663, 930, 1088
NmuCI GTSAC 3 cut(s) 549, 880, 1006
NspI RCATGY 1 cut(s) 498
NspV TTCGAA 1 cut(s) 848
PaeI GCATGC 1 cut(s) 498
PaqCI CACCTGC 1 cut(s) 20
PctI GAATGC 2 cut(s) 289, 604
PfeI GAWTC 2 cut(s) 845, 955
PflMI CCANNNNNTGG 2 cut(s) 446, 671
PfoI TCCNGGA 1 cut(s) 409
PkrI GCNGC 5 cut(s) 160, 406, 430, 520, 559
PleI GAGTC 1 cut(s) 933
PluTI GGCGCC 1 cut(s) 665
PpsI GAGTC 1 cut(s) 933
Ppu21I YACGTR 1 cut(s) 340
PshBI ATTAAT 1 cut(s) 792
Psp6I CCWGG 3 cut(s) 8, 409, 670
PspEI GGTNACC 1 cut(s) 549
PspFI CCCAGC 1 cut(s) 131
PspGI CCWGG 3 cut(s) 8, 409, 670
PspN4I GGNNCC 9 cut(s) 142, 219, 333, 362, 363, 408, 663, 930, 1088
PspOMI GGGCCC 1 cut(s) 361
PspPI GGNCC 6 cut(s) 116, 141, 206, 361, 362, 1019
PvuII CAGCTG 1 cut(s) 518
SalI GTCGAC 1 cut(s) 188
SaqAI TTAA 2 cut(s) 792, 1114
SatI GCNGC 5 cut(s) 159, 405, 429, 519, 558
Sau3AI GATC 4 cut(s) 87, 372, 685, 893
Sau96I GGNCC 6 cut(s) 116, 141, 206, 361, 362, 1019
SchI GAGTC 1 cut(s) 933
ScrFI CCNGG 3 cut(s) 10, 411, 672
SduI GDGCHC 2 cut(s) 365, 1091
SetI ASST 9 cut(s) 34, 56, 97, 342, 520, 595, 640, 805, 919
SfaNI GCATC 1 cut(s) 233
SfoI GGCGCC 1 cut(s) 663
SfuI TTCGAA 1 cut(s) 848
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
SpeI ACTAGT 1 cut(s) 770
SphI GCATGC 1 cut(s) 498
Sse9I AATT 5 cut(s) 16, 99, 165, 623, 851
SsiI CCGC 1 cut(s) 404
SspDI GGCGCC 1 cut(s) 661
SspMI CTAG 5 cut(s) 51, 609, 657, 771, 1040
StyD4I CCNGG 3 cut(s) 8, 409, 670
StyI CCWWGG 1 cut(s) 759
TaaI ACNGT 3 cut(s) 734, 880, 925
TaiI ACGT 1 cut(s) 342
TaqI TCGA 3 cut(s) 189, 356, 848
TasI AATT 5 cut(s) 16, 99, 165, 623, 851
TauI GCSGC 1 cut(s) 407
TfiI GAWTC 2 cut(s) 845, 955
Tru1I TTAA 2 cut(s) 792, 1114
Tru9I TTAA 2 cut(s) 792, 1114
TscAI CASTG 2 cut(s) 484, 739
TseFI GTSAC 3 cut(s) 549, 880, 1006
TseI GCWGC 4 cut(s) 158, 428, 518, 557
Tsp45I GTSAC 3 cut(s) 549, 880, 1006
TspDTI ATGAA 4 cut(s) 210, 611, 708, 827
TspGWI ACGGA 2 cut(s) 477, 648
TspRI CASTG 2 cut(s) 484, 739
Van91I CCANNNNNTGG 2 cut(s) 446, 671
VspI ATTAAT 1 cut(s) 792
XapI RAATTY 2 cut(s) 623, 851
XceI RCATGY 1 cut(s) 498
XmiI GTMKAC 1 cut(s) 189
XspI CTAG 5 cut(s) 51, 609, 657, 771, 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.