MD02G1311400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000520
Physical Location & Seq
Forward (+)
36654112 .. 36656152
2041 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1311400.v1.1.491

Sequence Viewer

Length: 510 bp
ATGATCAACCGATCCAAGGCCGAGGCCAAGGCCAGGTTCAAGGCTAAGGCCAAGGCCAAGTCGAAGTCCCTAGCCGGACGAGGATCCGGATCCTGGCAGGGGAAGATTCCAGGCCAACCGACGAAGCCCGGAGGTGAGATCCGGACTCCTCCCTCTTCTCATGATGGCCCTCCTACTCAACTCAAAAATCAAAAGGCTGTTGAAAAGAATCTGGCTAAAGTCCAGCTTGACAAGGAGAATTCTACTACTCCAAAACCAAAACCAGAAAAGAGAAAGAGCAGAATGGGTAGAACTTTCCTATTCACAGACCCTTGTCGAATTTGCAATGTGTTTGGGCACTGGACTGATTTATGCCCCTACCTGAAATTTGTCCCATACAACGTAACTAAGGTTGGCAAGGGCTATGAAATACATGACGGCCGCCATGGGAGGTATGCTGCTCCGATTTATTGTCTTAACTGTACGGTGCCGGTTGAACACGAGGCTGAAAAGTGCCCGTACGACAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

170

Amino Acids

18.81

Weight (kDa)

9.8

Isoelectric Point (pI)

27.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000520)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g08811 FvH4_2g13461
malus_domestica MD02G1305800.v1.1 MD02G1311300.v1.1 MD02G1311400.v1.1 MD03G1254800.v1.1 MD04G1165700.v1.1 MD05G1031200.v1.1 MD06G1150500.v1.1 MD06G1150600.v1.1 MD06G1150900.v1.1 MD06G1151100.v1.1 MD06G1151200.v1.1 MD07G1018900.v1.1 MD07G1019400.v1.1 MD07G1019500.v1.1 MD07G1023400.v1.1 MD08G1234000.v1.1 MD08G1234200.v1.1 MD13G1233200.v1.1 MD17G1268200.v1.1
pyrus_communis pycom02g25630 pycom02g26130 pycom05g02240 pycom05g02610 pycom06g13890 pycom06g13900 pycom07g01500 pycom07g01510 pycom07g01520 pycom07g01530 pycom07g01540 pycom08g17170 pycom08g17230 pycom08g20290 pycom13g20570
rosa_chinensis RchiOBHm_Chr2g0139011 RchiOBHm_Chr3g0492121
rosa_laevigata RLG00000002413 RLG00000019773 RLG00000019781 RLG00000022823 RLG00000024839 RLG00000027358
rosa_multiflora Rmu_sc0000379.1_g000005 Rmu_sc0002029.1_g000002 Rmu_sc0009440.1_g000007
rosa_roxburghii Rroxscaffold_2G00105770 Rroxscaffold_2G00105790 Rroxscaffold_2G00105890 Rroxscaffold_3G00241430 Rroxscaffold_4G00290390 Rroxscaffold_4G00290420 Rroxscaffold_7G00207210
rosa_rugosa Rorug02G0354100 Rorug02G0354200 Rorug02G0354300 Rorug02G0354400 Rorug02G0354500 Rorug03G0323900 Rorug07G0173300 Rorug07G0173400
rosa_samantha Rh1AG336500 Rh2AG403700 Rh2AG403900 Rh2BG414300 Rh2BG414400 Rh2BG474400 Rh2CG389800 Rh2CG390000 Rh2DG423900 Rh2DG424000 Rh2DG483000 Rh3CG333300 Rh3DG335400 Rh6AG108100 Rh6BG103000 Rh6BG103400 Rh6CG096600 Rh6DG090300 Rh7AG315900 Rh7BG306200 Rh7DG314100 Rh7DG314600
rosa_wichuraiana Rw2G032870 Rw7G026680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 466
AccIII TCCGGA 2 cut(s) 86, 141
AciI CCGC 1 cut(s) 421
AclWI GGATC 6 cut(s) 6, 78, 84, 91, 97, 133
AcoI YGGCCR 1 cut(s) 418
AcsI RAATTY 3 cut(s) 238, 318, 365
AfaI GTAC 2 cut(s) 463, 500
AfiI CCNNNNNNNGG 4 cut(s) 16, 33, 93, 99
AgsI TTSAA 3 cut(s) 40, 203, 476
AjnI CCWGG 3 cut(s) 32, 92, 109
AjuI GAANNNNNNNTTGG 2 cut(s) 20, 52
AluBI AGCT 2 cut(s) 226, 507
AluI AGCT 2 cut(s) 226, 507
AlwI GGATC 6 cut(s) 6, 78, 84, 91, 97, 133
Aor13HI TCCGGA 2 cut(s) 86, 141
AoxI GGCC 8 cut(s) 18, 24, 30, 48, 54, 112, 166, 418
ApeKI GCWGC 1 cut(s) 437
ApoI RAATTY 3 cut(s) 238, 318, 365
AspS9I GGNCC 1 cut(s) 167
AsuC2I CCSGG 1 cut(s) 129
AsuHPI GGTGA 1 cut(s) 146
BaeGI GKGCMC 2 cut(s) 339, 497
BamHI GGATCC 2 cut(s) 83, 89
BanI GGYRCC 1 cut(s) 466
BauI CACGAG 1 cut(s) 479
BbvI GCAGC 1 cut(s) 424
BccI CCATC 1 cut(s) 158
BceAI ACGGC 1 cut(s) 433
BciT130I CCWGG 3 cut(s) 34, 94, 111
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 1 cut(s) 129
BfaI CTAG 1 cut(s) 71
BisI GCNGC 2 cut(s) 421, 438
BlsI GCNGC 2 cut(s) 422, 439
Bme1390I CCNGG 4 cut(s) 34, 94, 111, 129
BmgT120I GGNCC 1 cut(s) 167
BmiI GGNNCC 3 cut(s) 85, 91, 468
BmrFI CCNGG 4 cut(s) 34, 94, 111, 129
BoxI GACNNNNGTC 1 cut(s) 312
Bpu10I CCTNAGC 1 cut(s) 45
BpuMI CCSGG 1 cut(s) 129
BsaBI GATNNNNATC 1 cut(s) 88
BsaJI CCNNGG 5 cut(s) 15, 21, 27, 51, 424
BsaWI WCCGGW 2 cut(s) 86, 141
Bsc4I CCNNNNNNNGG 4 cut(s) 16, 33, 93, 99
Bse118I RCCGGY 1 cut(s) 469
Bse1I ACTGG 1 cut(s) 344
Bse3DI GCAATG 1 cut(s) 331
Bse8I GATNNNNATC 1 cut(s) 88
BseAI TCCGGA 2 cut(s) 86, 141
BseBI CCWGG 3 cut(s) 34, 94, 111
BseDI CCNNGG 5 cut(s) 15, 21, 27, 51, 424
BseJI GATNNNNATC 1 cut(s) 88
BseLI CCNNNNNNNGG 4 cut(s) 16, 33, 93, 99
BseMI GCAATG 1 cut(s) 331
BseNI ACTGG 1 cut(s) 344
BseRI GAGGAG 1 cut(s) 138
BseSI GKGCMC 2 cut(s) 339, 497
BseX3I CGGCCG 1 cut(s) 418
BseXI GCAGC 1 cut(s) 424
Bsh1285I CGRYCG 1 cut(s) 421
BshFI GGCC 8 cut(s) 20, 26, 32, 50, 56, 114, 168, 420
BshNI GGYRCC 1 cut(s) 466
BsiEI CGRYCG 1 cut(s) 421
BsiSI CCGG 5 cut(s) 75, 87, 129, 142, 470
BsiWI CGTACG 1 cut(s) 498
BslFI GGGAC 2 cut(s) 52, 356
BslI CCNNNNNNNGG 4 cut(s) 16, 33, 93, 99
BsmFI GGGAC 2 cut(s) 52, 356
BsnI GGCC 8 cut(s) 20, 26, 32, 50, 56, 114, 168, 420
Bsp1286I GDGCHC 2 cut(s) 339, 497
Bsp13I TCCGGA 2 cut(s) 86, 141
Bsp143I GATC 5 cut(s) 3, 11, 83, 89, 138
Bsp19I CCATGG 1 cut(s) 424
BspACI CCGC 1 cut(s) 421
BspANI GGCC 8 cut(s) 20, 26, 32, 50, 56, 114, 168, 420
BspEI TCCGGA 2 cut(s) 86, 141
BspHI TCATGA 1 cut(s) 160
BspLI GGNNCC 3 cut(s) 85, 91, 468
BspPI GGATC 6 cut(s) 6, 78, 84, 91, 97, 133
BspT107I GGYRCC 1 cut(s) 466
BsrDI GCAATG 1 cut(s) 331
BsrFI RCCGGY 1 cut(s) 469
BsrI ACTGG 1 cut(s) 344
BssAI RCCGGY 1 cut(s) 469
BssECI CCNNGG 5 cut(s) 15, 21, 27, 51, 424
BssMI GATC 5 cut(s) 3, 11, 83, 89, 138
BssSI CACGAG 1 cut(s) 479
BssT1I CCWWGG 4 cut(s) 15, 27, 51, 424
Bst2BI CACGAG 1 cut(s) 479
Bst2UI CCWGG 3 cut(s) 34, 94, 111
Bst4CI ACNGT 2 cut(s) 461, 466
Bst6I CTCTTC 1 cut(s) 160
BstDEI CTNAG 2 cut(s) 45, 387
BstDSI CCRYGG 1 cut(s) 424
BstKTI GATC 5 cut(s) 6, 14, 86, 92, 141
BstMBI GATC 5 cut(s) 3, 11, 83, 89, 138
BstMCI CGRYCG 1 cut(s) 421
BstNI CCWGG 3 cut(s) 34, 94, 111
BstPAI GACNNNNGTC 1 cut(s) 312
BstSCI CCNGG 4 cut(s) 32, 92, 109, 127
BstSLI GKGCMC 2 cut(s) 339, 497
BstV1I GCAGC 1 cut(s) 424
BstX2I RGATCY 3 cut(s) 83, 89, 138
BstYI RGATCY 3 cut(s) 83, 89, 138
BstZI CGGCCG 1 cut(s) 418
BsuRI GGCC 8 cut(s) 20, 26, 32, 50, 56, 114, 168, 420
BtgI CCRYGG 1 cut(s) 424
BtsIMutI CAGTG 1 cut(s) 337
CciI TCATGA 1 cut(s) 160
Cfr10I RCCGGY 1 cut(s) 469
Cfr13I GGNCC 1 cut(s) 167
Csp6I GTAC 2 cut(s) 462, 499
CviAII CATG 3 cut(s) 161, 413, 425
CviQI GTAC 2 cut(s) 462, 499
DdeI CTNAG 2 cut(s) 45, 387
DpnI GATC 5 cut(s) 5, 13, 85, 91, 140
DpnII GATC 5 cut(s) 3, 11, 83, 89, 138
EaeI YGGCCR 1 cut(s) 418
EagI CGGCCG 1 cut(s) 418
Eam1104I CTCTTC 1 cut(s) 160
EarI CTCTTC 1 cut(s) 160
EclXI CGGCCG 1 cut(s) 418
Eco130I CCWWGG 4 cut(s) 15, 27, 51, 424
Eco52I CGGCCG 1 cut(s) 418
EcoRI GAATTC 1 cut(s) 238
EcoRII CCWGG 3 cut(s) 32, 92, 109
EcoT14I CCWWGG 4 cut(s) 15, 27, 51, 424
ErhI CCWWGG 4 cut(s) 15, 27, 51, 424
FaeI CATG 3 cut(s) 164, 416, 428
FaiI YATR 7 cut(s) 162, 352, 376, 405, 414, 426, 435
FalI AAGNNNNNCTT 2 cut(s) 210, 242
FaqI GGGAC 2 cut(s) 52, 356
FatI CATG 3 cut(s) 160, 412, 424
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 2 cut(s) 421, 438
Fsp4HI GCNGC 2 cut(s) 421, 438
FspBI CTAG 1 cut(s) 71
GluI GCNGC 2 cut(s) 421, 438
HaeIII GGCC 8 cut(s) 20, 26, 32, 50, 56, 114, 168, 420
HapII CCGG 5 cut(s) 75, 87, 129, 142, 470
Hin1II CATG 3 cut(s) 164, 416, 428
HinfI GANTC 3 cut(s) 106, 145, 208
HpaII CCGG 5 cut(s) 75, 87, 129, 142, 470
HphI GGTGA 1 cut(s) 146
Hpy188I TCNGA 1 cut(s) 444
Hpy188III TCNNGA 3 cut(s) 87, 142, 161
Hpy99I CGWCG 1 cut(s) 124
HpyCH4III ACNGT 2 cut(s) 461, 466
HpyCH4IV ACGT 1 cut(s) 381
HpyCH4V TGCA 1 cut(s) 324
HpyF3I CTNAG 2 cut(s) 45, 387
HpySE526I ACGT 1 cut(s) 381
Hsp92II CATG 3 cut(s) 164, 416, 428
Kpn2I TCCGGA 2 cut(s) 86, 141
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 5 cut(s) 3, 11, 83, 89, 138
LmnI GCTCC 1 cut(s) 445
Lsp1109I GCAGC 1 cut(s) 424
MaeI CTAG 1 cut(s) 71
MaeII ACGT 1 cut(s) 381
MaeIII GTNAC 1 cut(s) 382
MalI GATC 5 cut(s) 5, 13, 85, 91, 140
MboI GATC 5 cut(s) 3, 11, 83, 89, 138
MboII GAAGA 2 cut(s) 115, 147
MflI RGATCY 3 cut(s) 83, 89, 138
MhlI GDGCHC 2 cut(s) 339, 497
MluCI AATT 3 cut(s) 238, 318, 365
MlyI GAGTC 1 cut(s) 139
MnlI CCTC 8 cut(s) 16, 74, 125, 159, 163, 180, 423, 475
MroI TCCGGA 2 cut(s) 86, 141
MseI TTAA 1 cut(s) 456
MspA1I CMGCKG 1 cut(s) 507
MspI CCGG 5 cut(s) 75, 87, 129, 142, 470
MspR9I CCNGG 4 cut(s) 34, 94, 111, 129
MvaI CCWGG 3 cut(s) 34, 94, 111
NciI CCSGG 1 cut(s) 129
NcoI CCATGG 1 cut(s) 424
NdeII GATC 5 cut(s) 3, 11, 83, 89, 138
NlaIII CATG 3 cut(s) 164, 416, 428
NlaIV GGNNCC 3 cut(s) 85, 91, 468
NmeAIII GCCGAG 1 cut(s) 46
PagI TCATGA 1 cut(s) 160
PfeI GAWTC 2 cut(s) 106, 208
Pfl23II CGTACG 1 cut(s) 498
PkrI GCNGC 2 cut(s) 422, 439
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
PshAI GACNNNNGTC 1 cut(s) 312
Psp6I CCWGG 3 cut(s) 32, 92, 109
PspGI CCWGG 3 cut(s) 32, 92, 109
PspLI CGTACG 1 cut(s) 498
PspN4I GGNNCC 3 cut(s) 85, 91, 468
PspPI GGNCC 1 cut(s) 167
PsuI RGATCY 3 cut(s) 83, 89, 138
PvuII CAGCTG 1 cut(s) 507
RsaI GTAC 2 cut(s) 463, 500
RsaNI GTAC 2 cut(s) 462, 499
SaqAI TTAA 1 cut(s) 456
SatI GCNGC 2 cut(s) 421, 438
Sau3AI GATC 5 cut(s) 3, 11, 83, 89, 138
Sau96I GGNCC 1 cut(s) 167
SchI GAGTC 1 cut(s) 139
ScrFI CCNGG 4 cut(s) 34, 94, 111, 129
SduI GDGCHC 2 cut(s) 339, 497
SetI ASST 8 cut(s) 38, 136, 228, 363, 384, 393, 434, 509
Sse9I AATT 3 cut(s) 238, 318, 365
SsiI CCGC 1 cut(s) 421
SspMI CTAG 1 cut(s) 71
StyD4I CCNGG 4 cut(s) 32, 92, 109, 127
StyI CCWWGG 4 cut(s) 15, 27, 51, 424
TaaI ACNGT 2 cut(s) 461, 466
TaiI ACGT 1 cut(s) 384
TaqI TCGA 2 cut(s) 62, 316
TasI AATT 3 cut(s) 238, 318, 365
TauI GCSGC 1 cut(s) 423
TfiI GAWTC 2 cut(s) 106, 208
Tru1I TTAA 1 cut(s) 456
Tru9I TTAA 1 cut(s) 456
TscAI CASTG 1 cut(s) 344
TseI GCWGC 1 cut(s) 437
TspDTI ATGAA 1 cut(s) 420
TspRI CASTG 1 cut(s) 344
XapI RAATTY 3 cut(s) 238, 318, 365
XspI CTAG 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.