MD03G1254800.v1.1

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000520
Physical Location & Seq
Forward (+)
34127221 .. 34128596
1376 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1254800.v1.1.491

Sequence Viewer

Length: 753 bp
ATGAACGACCGCCGTCTCAGAACCGGATCAGACTCCGAGAAGAAGGCCAATTCTTCCCTAAAAGTGGGAAGCGGCTCAGAGTACGAGTCTCGCAAGAAAAAAAATCTAAAAGGGGGGATAAAAAATCCAAAAGGGGGGTTCCAAATCCATTATATTACGGAGGAGGAGAAACAAGCCGCGATTGCAGAGTACTACGCATGGGAAGCGCAAGAGCAAGAGCGATTCCCTCACTTAAAAGAAGGAAGGCAACTTGTGTTGGTGGTGCCTGATGAGGAGAATATAGACAAGCCTGTTTTAGACTCTGTGACTGATCAGCTCAATACTACCCCGACTTGTGAGGAGAAAAGTCGTTGCACGGGCAATCACAAACAAACAGATGATGAGGAGAAGGGGCCTCTATGTCGAATTTGCCGTGATGGTCACCATGGGCTTGAGTACTGCCCGTGGCGTGGACGTGTCCCGATGGATGCAACTGAAGTTGGCAAGGGCTTTGAAATAACTTGTCATTTTTGTGGTAGTTTGGATAACAAGTGCAAGCATAATTGGAGCCATGCTGTCGAGGTTTTCTGTCGTATTTGTAATGTGGAAGGAGACCACTCGGAGCTTGAGTGCCCAAAGCTACCAGAGATAGCCAAAGAGGAGGCAAGAGTGGAAGCCAAAAAAGCAAAGAAGAAAGCAGCCAAAGCCAGGGCAAGGGCCAGAGCCAAAGTTCTTAAAGACGGACAGTTGGGTTCCTCCACCATCACTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

251

Amino Acids

28.11

Weight (kDa)

8.19

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000520)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g08811 FvH4_2g13461
malus_domestica MD02G1305800.v1.1 MD02G1311300.v1.1 MD02G1311400.v1.1 MD03G1254800.v1.1 MD04G1165700.v1.1 MD05G1031200.v1.1 MD06G1150500.v1.1 MD06G1150600.v1.1 MD06G1150900.v1.1 MD06G1151100.v1.1 MD06G1151200.v1.1 MD07G1018900.v1.1 MD07G1019400.v1.1 MD07G1019500.v1.1 MD07G1023400.v1.1 MD08G1234000.v1.1 MD08G1234200.v1.1 MD13G1233200.v1.1 MD17G1268200.v1.1
pyrus_communis pycom02g25630 pycom02g26130 pycom05g02240 pycom05g02610 pycom06g13890 pycom06g13900 pycom07g01500 pycom07g01510 pycom07g01520 pycom07g01530 pycom07g01540 pycom08g17170 pycom08g17230 pycom08g20290 pycom13g20570
rosa_chinensis RchiOBHm_Chr2g0139011 RchiOBHm_Chr3g0492121
rosa_laevigata RLG00000002413 RLG00000019773 RLG00000019781 RLG00000022823 RLG00000024839 RLG00000027358
rosa_multiflora Rmu_sc0000379.1_g000005 Rmu_sc0002029.1_g000002 Rmu_sc0009440.1_g000007
rosa_roxburghii Rroxscaffold_2G00105770 Rroxscaffold_2G00105790 Rroxscaffold_2G00105890 Rroxscaffold_3G00241430 Rroxscaffold_4G00290390 Rroxscaffold_4G00290420 Rroxscaffold_7G00207210
rosa_rugosa Rorug02G0354100 Rorug02G0354200 Rorug02G0354300 Rorug02G0354400 Rorug02G0354500 Rorug03G0323900 Rorug07G0173300 Rorug07G0173400
rosa_samantha Rh1AG336500 Rh2AG403700 Rh2AG403900 Rh2BG414300 Rh2BG414400 Rh2BG474400 Rh2CG389800 Rh2CG390000 Rh2DG423900 Rh2DG424000 Rh2DG483000 Rh3CG333300 Rh3DG335400 Rh6AG108100 Rh6BG103000 Rh6BG103400 Rh6CG096600 Rh6DG090300 Rh7AG315900 Rh7BG306200 Rh7DG314100 Rh7DG314600
rosa_wichuraiana Rw2G032870 Rw7G026680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 262
AccII CGCG 1 cut(s) 179
AciI CCGC 3 cut(s) 10, 72, 177
AclWI GGATC 1 cut(s) 34
AcsI RAATTY 1 cut(s) 405
AcuI CTGAAG 1 cut(s) 495
AfaI GTAC 3 cut(s) 83, 191, 437
AfiI CCNNNNNNNGG 5 cut(s) 64, 134, 449, 687, 693
AflIII ACRYGT 1 cut(s) 454
AgsI TTSAA 1 cut(s) 494
AjiI CACGTC 1 cut(s) 455
AjnI CCWGG 1 cut(s) 686
AluBI AGCT 3 cut(s) 316, 604, 619
AluI AGCT 3 cut(s) 316, 604, 619
Alw26I GTCTC 3 cut(s) 20, 93, 585
AlwI GGATC 1 cut(s) 34
AoxI GGCC 3 cut(s) 45, 392, 696
ApeKI GCWGC 1 cut(s) 677
ApoI RAATTY 1 cut(s) 405
AspLEI GCGC 1 cut(s) 208
AspS9I GGNCC 2 cut(s) 392, 696
AsuHPI GGTGA 1 cut(s) 413
BaeGI GKGCMC 1 cut(s) 614
BanI GGYRCC 1 cut(s) 262
BbvI GCAGC 1 cut(s) 689
BccI CCATC 3 cut(s) 410, 457, 749
BceAI ACGGC 1 cut(s) 396
BciT130I CCWGG 1 cut(s) 688
BclI TGATCA 1 cut(s) 310
BcoDI GTCTC 3 cut(s) 20, 93, 585
BisI GCNGC 3 cut(s) 73, 177, 678
BlsI GCNGC 3 cut(s) 74, 178, 679
BmcAI AGTACT 2 cut(s) 191, 437
Bme1390I CCNGG 1 cut(s) 688
BmgBI CACGTC 1 cut(s) 455
BmgT120I GGNCC 2 cut(s) 392, 696
BmiI GGNNCC 5 cut(s) 140, 264, 393, 548, 733
BmrFI CCNGG 1 cut(s) 688
BmsI GCATC 1 cut(s) 457
BoxI GACNNNNGTC 1 cut(s) 12
BpuEI CTTGAG 2 cut(s) 452, 626
BsaI GGTCTC 1 cut(s) 585
BsaJI CCNNGG 3 cut(s) 424, 443, 687
BsaWI WCCGGW 1 cut(s) 23
BsaXI ACNNNNNCTCC 2 cut(s) 593, 623
Bsc4I CCNNNNNNNGG 5 cut(s) 64, 134, 449, 687, 693
BseBI CCWGG 1 cut(s) 688
BseDI CCNNGG 3 cut(s) 424, 443, 687
BseGI GGATG 1 cut(s) 472
BseLI CCNNNNNNNGG 5 cut(s) 64, 134, 449, 687, 693
BseMII CTCAG 2 cut(s) 31, 90
BseRI GAGGAG 6 cut(s) 176, 179, 287, 353, 398, 653
BseSI GKGCMC 1 cut(s) 614
BseXI GCAGC 1 cut(s) 689
Bsh1236I CGCG 1 cut(s) 179
Bsh1285I CGRYCG 1 cut(s) 10
BshFI GGCC 3 cut(s) 47, 394, 698
BshNI GGYRCC 1 cut(s) 262
BsiEI CGRYCG 1 cut(s) 10
BsiSI CCGG 1 cut(s) 24
BslFI GGGAC 1 cut(s) 443
BslI CCNNNNNNNGG 5 cut(s) 64, 134, 449, 687, 693
BsmAI GTCTC 3 cut(s) 20, 93, 585
BsmBI CGTCTC 1 cut(s) 20
BsmFI GGGAC 1 cut(s) 443
BsnI GGCC 3 cut(s) 47, 394, 698
Bso31I GGTCTC 1 cut(s) 585
Bsp1286I GDGCHC 1 cut(s) 614
Bsp143I GATC 2 cut(s) 26, 310
Bsp19I CCATGG 1 cut(s) 424
BspACI CCGC 3 cut(s) 10, 72, 177
BspANI GGCC 3 cut(s) 47, 394, 698
BspCNI CTCAG 2 cut(s) 30, 89
BspFNI CGCG 1 cut(s) 179
BspLI GGNNCC 5 cut(s) 140, 264, 393, 548, 733
BspPI GGATC 1 cut(s) 34
BspT107I GGYRCC 1 cut(s) 262
BspTNI GGTCTC 1 cut(s) 585
BssECI CCNNGG 3 cut(s) 424, 443, 687
BssMI GATC 2 cut(s) 26, 310
BssT1I CCWWGG 1 cut(s) 424
Bst2UI CCWGG 1 cut(s) 688
Bst4CI ACNGT 1 cut(s) 726
BstC8I GCNNGC 1 cut(s) 536
BstDEI CTNAG 2 cut(s) 17, 76
BstDSI CCRYGG 2 cut(s) 424, 443
BstEII GGTNACC 1 cut(s) 419
BstF5I GGATG 1 cut(s) 472
BstFNI CGCG 1 cut(s) 179
BstHHI GCGC 1 cut(s) 208
BstKTI GATC 2 cut(s) 29, 313
BstMAI GTCTC 3 cut(s) 20, 93, 585
BstMBI GATC 2 cut(s) 26, 310
BstMCI CGRYCG 1 cut(s) 10
BstMWI GCNNNNNNNGC 4 cut(s) 182, 203, 662, 683
BstNI CCWGG 1 cut(s) 688
BstPAI GACNNNNGTC 1 cut(s) 12
BstPI GGTNACC 1 cut(s) 419
BstSCI CCNGG 1 cut(s) 686
BstSLI GKGCMC 1 cut(s) 614
BstUI CGCG 1 cut(s) 179
BstV1I GCAGC 1 cut(s) 689
BsuRI GGCC 3 cut(s) 47, 394, 698
BtgI CCRYGG 2 cut(s) 424, 443
BtrI CACGTC 1 cut(s) 455
BtsCI GGATG 1 cut(s) 472
Cac8I GCNNGC 1 cut(s) 536
CfoI GCGC 1 cut(s) 208
Cfr13I GGNCC 2 cut(s) 392, 696
Csp6I GTAC 3 cut(s) 82, 190, 436
CviAII CATG 3 cut(s) 198, 425, 551
CviQI GTAC 3 cut(s) 82, 190, 436
DdeI CTNAG 2 cut(s) 17, 76
DpnI GATC 2 cut(s) 28, 312
DpnII GATC 2 cut(s) 26, 310
Eco130I CCWWGG 1 cut(s) 424
Eco31I GGTCTC 1 cut(s) 585
Eco57I CTGAAG 1 cut(s) 495
Eco91I GGTNACC 1 cut(s) 419
EcoO109I RGGNCCY 1 cut(s) 392
EcoO65I GGTNACC 1 cut(s) 419
EcoRII CCWGG 1 cut(s) 686
EcoT14I CCWWGG 1 cut(s) 424
ErhI CCWWGG 1 cut(s) 424
Esp3I CGTCTC 1 cut(s) 20
FaeI CATG 3 cut(s) 201, 428, 554
FaiI YATR 7 cut(s) 153, 199, 281, 400, 426, 540, 552
FaqI GGGAC 1 cut(s) 443
FatI CATG 3 cut(s) 197, 424, 550
FbaI TGATCA 1 cut(s) 310
Fnu4HI GCNGC 3 cut(s) 73, 177, 678
FokI GGATG 1 cut(s) 479
Fsp4HI GCNGC 3 cut(s) 73, 177, 678
GlaI GCGC 1 cut(s) 207
GluI GCNGC 3 cut(s) 73, 177, 678
HaeIII GGCC 3 cut(s) 47, 394, 698
HapII CCGG 1 cut(s) 24
HhaI GCGC 1 cut(s) 208
Hin1II CATG 3 cut(s) 201, 428, 554
Hin6I GCGC 1 cut(s) 206
HinP1I GCGC 1 cut(s) 206
HinfI GANTC 4 cut(s) 32, 86, 222, 299
HpaII CCGG 1 cut(s) 24
HphI GGTGA 1 cut(s) 413
Hpy166II GTNNAC 1 cut(s) 452
Hpy188I TCNGA 5 cut(s) 20, 31, 37, 79, 601
Hpy188III TCNNGA 1 cut(s) 460
Hpy8I GTNNAC 1 cut(s) 452
HpyAV CCTTC 5 cut(s) 37, 233, 237, 382, 581
HpyCH4III ACNGT 1 cut(s) 726
HpyCH4IV ACGT 1 cut(s) 454
HpyCH4V TGCA 4 cut(s) 185, 354, 470, 534
HpyF10VI GCNNNNNNNGC 4 cut(s) 182, 203, 662, 683
HpyF3I CTNAG 2 cut(s) 17, 76
HpySE526I ACGT 1 cut(s) 454
Hsp92II CATG 3 cut(s) 201, 428, 554
HspAI GCGC 1 cut(s) 206
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 2 cut(s) 26, 310
LmnI GCTCC 2 cut(s) 546, 601
LpnPI CCDG 7 cut(s) 37, 279, 303, 636, 673, 700, 712
Lsp1109I GCAGC 1 cut(s) 689
LweI GCATC 1 cut(s) 457
MaeII ACGT 1 cut(s) 454
MaeIII GTNAC 2 cut(s) 304, 419
MalI GATC 2 cut(s) 28, 312
MboI GATC 2 cut(s) 26, 310
MboII GAAGA 3 cut(s) 45, 52, 682
MhlI GDGCHC 1 cut(s) 614
MluCI AATT 3 cut(s) 49, 405, 541
MlyI GAGTC 3 cut(s) 26, 95, 293
MseI TTAA 2 cut(s) 233, 714
MslI CAYNNNNRTG 1 cut(s) 510
MspI CCGG 1 cut(s) 24
MspR9I CCNGG 1 cut(s) 688
MvaI CCWGG 1 cut(s) 688
MvnI CGCG 1 cut(s) 179
MwoI GCNNNNNNNGC 4 cut(s) 182, 203, 662, 683
NcoI CCATGG 1 cut(s) 424
NdeII GATC 2 cut(s) 26, 310
NlaIII CATG 3 cut(s) 201, 428, 554
NlaIV GGNNCC 5 cut(s) 140, 264, 393, 548, 733
NmuCI GTSAC 2 cut(s) 304, 419
PcsI WCGNNNNNNNCGW 1 cut(s) 409
PfeI GAWTC 1 cut(s) 222
PkrI GCNGC 3 cut(s) 74, 178, 679
PleI GAGTC 3 cut(s) 26, 94, 293
PpsI GAGTC 3 cut(s) 26, 94, 293
PshAI GACNNNNGTC 1 cut(s) 12
Psp6I CCWGG 1 cut(s) 686
PspEI GGTNACC 1 cut(s) 419
PspGI CCWGG 1 cut(s) 686
PspN4I GGNNCC 5 cut(s) 140, 264, 393, 548, 733
PspPI GGNCC 2 cut(s) 392, 696
RsaI GTAC 3 cut(s) 83, 191, 437
RsaNI GTAC 3 cut(s) 82, 190, 436
RseI CAYNNNNRTG 1 cut(s) 510
SaqAI TTAA 2 cut(s) 233, 714
SatI GCNGC 3 cut(s) 73, 177, 678
Sau3AI GATC 2 cut(s) 26, 310
Sau96I GGNCC 2 cut(s) 392, 696
ScaI AGTACT 2 cut(s) 191, 437
SchI GAGTC 3 cut(s) 26, 95, 293
ScrFI CCNGG 1 cut(s) 688
SduI GDGCHC 1 cut(s) 614
SetI ASST 5 cut(s) 318, 457, 564, 606, 621
SfaNI GCATC 1 cut(s) 457
SmiMI CAYNNNNRTG 1 cut(s) 510
SmlI CTYRAG 2 cut(s) 431, 605
SmoI CTYRAG 2 cut(s) 431, 605
Sse9I AATT 3 cut(s) 49, 405, 541
SsiI CCGC 3 cut(s) 10, 72, 177
StyD4I CCNGG 1 cut(s) 686
StyI CCWWGG 1 cut(s) 424
TaaI ACNGT 1 cut(s) 726
TaiI ACGT 1 cut(s) 457
TaqI TCGA 2 cut(s) 403, 558
TasI AATT 3 cut(s) 49, 405, 541
TatI WGTACW 2 cut(s) 189, 435
TauI GCSGC 2 cut(s) 75, 179
TfiI GAWTC 1 cut(s) 222
Tru1I TTAA 2 cut(s) 233, 714
Tru9I TTAA 2 cut(s) 233, 714
TseFI GTSAC 2 cut(s) 304, 419
TseI GCWGC 1 cut(s) 677
Tsp45I GTSAC 2 cut(s) 304, 419
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 173, 735
XapI RAATTY 1 cut(s) 405
ZrmI AGTACT 2 cut(s) 191, 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.