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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 3 cut(s) | 70, 318, 491 |
| AclWI | GGATC | 1 cut(s) | 84 |
| AcoI | YGGCCR | 1 cut(s) | 328 |
| AcsI | RAATTY | 2 cut(s) | 9, 293 |
| AcvI | CACGTG | 1 cut(s) | 362 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 532, 545 |
| AflIII | ACRYGT | 2 cut(s) | 359, 361 |
| AgsI | TTSAA | 3 cut(s) | 191, 292, 337 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 80 |
| AlwI | GGATC | 1 cut(s) | 84 |
| AoxI | GGCC | 2 cut(s) | 328, 534 |
| ApoI | RAATTY | 2 cut(s) | 9, 293 |
| AspLEI | GCGC | 1 cut(s) | 269 |
| AspS9I | GGNCC | 4 cut(s) | 67, 113, 535, 548 |
| AsuHPI | GGTGA | 3 cut(s) | 76, 431, 547 |
| AvaII | GGWCC | 3 cut(s) | 67, 113, 548 |
| BalI | TGGCCA | 1 cut(s) | 330 |
| BbrPI | CACGTG | 1 cut(s) | 362 |
| BccI | CCATC | 2 cut(s) | 433, 480 |
| BceAI | ACGGC | 1 cut(s) | 549 |
| BfmI | CTRYAG | 1 cut(s) | 543 |
| Bme18I | GGWCC | 3 cut(s) | 67, 113, 548 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 80 |
| BmgT120I | GGNCC | 4 cut(s) | 67, 113, 535, 548 |
| BmiI | GGNNCC | 1 cut(s) | 537 |
| BsaAI | YACGTR | 1 cut(s) | 362 |
| BsaJI | CCNNGG | 3 cut(s) | 60, 381, 415 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 523, 553 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 532, 545 |
| Bse3DI | GCAATG | 1 cut(s) | 507 |
| BseDI | CCNNGG | 3 cut(s) | 60, 381, 415 |
| BseGI | GGATG | 1 cut(s) | 444 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 532, 545 |
| BseMI | GCAATG | 1 cut(s) | 507 |
| BseRI | GAGGAG | 1 cut(s) | 236 |
| BshFI | GGCC | 2 cut(s) | 330, 536 |
| BsiSI | CCGG | 1 cut(s) | 111 |
| BslFI | GGGAC | 1 cut(s) | 88 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 532, 545 |
| BsmFI | GGGAC | 1 cut(s) | 88 |
| BsnI | GGCC | 2 cut(s) | 330, 536 |
| Bsp143I | GATC | 1 cut(s) | 89 |
| Bsp19I | CCATGG | 1 cut(s) | 60 |
| BspACI | CCGC | 3 cut(s) | 70, 318, 491 |
| BspANI | GGCC | 2 cut(s) | 330, 536 |
| BspLI | GGNNCC | 1 cut(s) | 537 |
| BspPI | GGATC | 1 cut(s) | 84 |
| BsrDI | GCAATG | 1 cut(s) | 507 |
| BssECI | CCNNGG | 3 cut(s) | 60, 381, 415 |
| BssMI | GATC | 1 cut(s) | 89 |
| BssT1I | CCWWGG | 1 cut(s) | 60 |
| Bst6I | CTCTTC | 1 cut(s) | 243 |
| BstBAI | YACGTR | 1 cut(s) | 362 |
| BstC8I | GCNNGC | 3 cut(s) | 17, 429, 491 |
| BstDSI | CCRYGG | 2 cut(s) | 60, 415 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 543 |
| BstF5I | GGATG | 1 cut(s) | 444 |
| BstHHI | GCGC | 1 cut(s) | 269 |
| BstKTI | GATC | 1 cut(s) | 92 |
| BstMBI | GATC | 1 cut(s) | 89 |
| BstNSI | RCATGY | 1 cut(s) | 431 |
| BstSFI | CTRYAG | 1 cut(s) | 543 |
| BstX2I | RGATCY | 1 cut(s) | 89 |
| BstYI | RGATCY | 1 cut(s) | 89 |
| BsuRI | GGCC | 2 cut(s) | 330, 536 |
| BtgI | CCRYGG | 2 cut(s) | 60, 415 |
| BtsCI | GGATG | 1 cut(s) | 444 |
| Cac8I | GCNNGC | 3 cut(s) | 17, 429, 491 |
| CfoI | GCGC | 1 cut(s) | 269 |
| Cfr13I | GGNCC | 4 cut(s) | 67, 113, 535, 548 |
| CpoI | CGGWCCG | 1 cut(s) | 113 |
| CspI | CGGWCCG | 1 cut(s) | 113 |
| CviAII | CATG | 3 cut(s) | 61, 355, 428 |
| CviJI | RGCY | 6 cut(s) | 146, 203, 330, 396, 501, 536 |
| CviKI_1 | RGCY | 6 cut(s) | 146, 203, 330, 396, 501, 536 |
| DpnI | GATC | 1 cut(s) | 91 |
| DpnII | GATC | 1 cut(s) | 89 |
| DriI | GACNNNNNGTC | 1 cut(s) | 80 |
| EaeI | YGGCCR | 1 cut(s) | 328 |
| Eam1104I | CTCTTC | 1 cut(s) | 243 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 80 |
| EarI | CTCTTC | 1 cut(s) | 243 |
| Eco130I | CCWWGG | 1 cut(s) | 60 |
| Eco47I | GGWCC | 3 cut(s) | 67, 113, 548 |
| Eco72I | CACGTG | 1 cut(s) | 362 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 543 |
| EcoO109I | RGGNCCY | 1 cut(s) | 548 |
| EcoT14I | CCWWGG | 1 cut(s) | 60 |
| ErhI | CCWWGG | 1 cut(s) | 60 |
| FaeI | CATG | 3 cut(s) | 64, 358, 431 |
| FaiI | YATR | 9 cut(s) | 62, 134, 200, 356, 399, 429, 445, 459, 495 |
| FaqI | GGGAC | 1 cut(s) | 88 |
| FatI | CATG | 3 cut(s) | 60, 354, 427 |
| FauI | CCCGC | 1 cut(s) | 498 |
| FokI | GGATG | 1 cut(s) | 451 |
| GlaI | GCGC | 1 cut(s) | 268 |
| HaeIII | GGCC | 2 cut(s) | 330, 536 |
| HapII | CCGG | 1 cut(s) | 111 |
| HhaI | GCGC | 1 cut(s) | 269 |
| Hin1II | CATG | 3 cut(s) | 64, 358, 431 |
| Hin6I | GCGC | 1 cut(s) | 267 |
| HinP1I | GCGC | 1 cut(s) | 267 |
| HinfI | GANTC | 2 cut(s) | 75, 155 |
| HpaII | CCGG | 1 cut(s) | 111 |
| HphI | GGTGA | 3 cut(s) | 76, 431, 547 |
| Hpy188I | TCNGA | 4 cut(s) | 128, 212, 477, 531 |
| Hpy188III | TCNNGA | 2 cut(s) | 191, 561 |
| HpyAV | CCTTC | 2 cut(s) | 421, 536 |
| HpyCH4IV | ACGT | 1 cut(s) | 361 |
| HpyCH4V | TGCA | 1 cut(s) | 262 |
| HpySE526I | ACGT | 1 cut(s) | 361 |
| Hsp92II | CATG | 3 cut(s) | 64, 358, 431 |
| HspAI | GCGC | 1 cut(s) | 267 |
| Kzo9I | GATC | 1 cut(s) | 89 |
| LpnPI | CCDG | 4 cut(s) | 100, 124, 258, 555 |
| MaeII | ACGT | 1 cut(s) | 361 |
| MaeIII | GTNAC | 3 cut(s) | 82, 376, 406 |
| MalI | GATC | 1 cut(s) | 91 |
| MboI | GATC | 1 cut(s) | 89 |
| MboII | GAAGA | 3 cut(s) | 130, 148, 260 |
| MflI | RGATCY | 1 cut(s) | 89 |
| MlsI | TGGCCA | 1 cut(s) | 330 |
| MluCI | AATT | 3 cut(s) | 9, 94, 293 |
| MluNI | TGGCCA | 1 cut(s) | 330 |
| MlyI | GAGTC | 2 cut(s) | 69, 149 |
| MmeI | TCCRAC | 2 cut(s) | 185, 235 |
| Mox20I | TGGCCA | 1 cut(s) | 330 |
| MscI | TGGCCA | 1 cut(s) | 330 |
| MseI | TTAA | 2 cut(s) | 150, 216 |
| MslI | CAYNNNNRTG | 2 cut(s) | 336, 462 |
| Msp20I | TGGCCA | 1 cut(s) | 330 |
| MspI | CCGG | 1 cut(s) | 111 |
| NcoI | CCATGG | 1 cut(s) | 60 |
| NdeII | GATC | 1 cut(s) | 89 |
| NlaIII | CATG | 3 cut(s) | 64, 358, 431 |
| NlaIV | GGNNCC | 1 cut(s) | 537 |
| NmuCI | GTSAC | 1 cut(s) | 82 |
| NspI | RCATGY | 1 cut(s) | 431 |
| PaeI | GCATGC | 1 cut(s) | 431 |
| PleI | GAGTC | 2 cut(s) | 69, 149 |
| PmaCI | CACGTG | 1 cut(s) | 362 |
| PmlI | CACGTG | 1 cut(s) | 362 |
| PpsI | GAGTC | 2 cut(s) | 69, 149 |
| Ppu21I | YACGTR | 1 cut(s) | 362 |
| PpuMI | RGGWCCY | 1 cut(s) | 548 |
| Psp5II | RGGWCCY | 1 cut(s) | 548 |
| PspCI | CACGTG | 1 cut(s) | 362 |
| PspN4I | GGNNCC | 1 cut(s) | 537 |
| PspPI | GGNCC | 4 cut(s) | 67, 113, 535, 548 |
| PspPPI | RGGWCCY | 1 cut(s) | 548 |
| PsuI | RGATCY | 1 cut(s) | 89 |
| RseI | CAYNNNNRTG | 2 cut(s) | 336, 462 |
| Rsr2I | CGGWCCG | 1 cut(s) | 113 |
| RsrII | CGGWCCG | 1 cut(s) | 113 |
| SaqAI | TTAA | 2 cut(s) | 150, 216 |
| Sau3AI | GATC | 1 cut(s) | 89 |
| Sau96I | GGNCC | 4 cut(s) | 67, 113, 535, 548 |
| SchI | GAGTC | 2 cut(s) | 69, 149 |
| SetI | ASST | 7 cut(s) | 198, 289, 364, 387, 413, 550, 560 |
| SfcI | CTRYAG | 1 cut(s) | 543 |
| SinI | GGWCC | 3 cut(s) | 67, 113, 548 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 336, 462 |
| SphI | GCATGC | 1 cut(s) | 431 |
| Sse9I | AATT | 3 cut(s) | 9, 94, 293 |
| SsiI | CCGC | 3 cut(s) | 70, 318, 491 |
| StyI | CCWWGG | 1 cut(s) | 60 |
| TaiI | ACGT | 1 cut(s) | 364 |
| TaqI | TCGA | 1 cut(s) | 78 |
| TaqII | GACCGA | 1 cut(s) | 130 |
| TasI | AATT | 3 cut(s) | 9, 94, 293 |
| Tru1I | TTAA | 2 cut(s) | 150, 216 |
| Tru9I | TTAA | 2 cut(s) | 150, 216 |
| TseFI | GTSAC | 1 cut(s) | 82 |
| Tsp45I | GTSAC | 1 cut(s) | 82 |
| TspDTI | ATGAA | 3 cut(s) | 149, 192, 414 |
| TspGWI | ACGGA | 1 cut(s) | 404 |
| VpaK11BI | GGWCC | 3 cut(s) | 67, 113, 548 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 543 |
| XapI | RAATTY | 2 cut(s) | 9, 293 |
| XceI | RCATGY | 1 cut(s) | 431 |