Rh7BG306200

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0000520
Physical Location & Seq
Reverse (-)
29208134 .. 29210002
1869 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG306200.1

Sequence Viewer

Length: 723 bp
ATGTCAATGTCTATGACGATAGAGAAACCAGCCGGCAAGGAGGTGGGCTGCGACCCGGCGCAACTGGCGAAACGTGGATGGCTGAACGTCGACAAGGACGACGACGATGACGTCAGAGAGCCGACCTGCATAAGTATGTTGCCAAGGAAAAACAAGGACGACGACGAGGAGATCTGGGTTCCGGCAAAGTACTGTAACAAGCCAGAAGAACCTCCTAGTGATGAAGAGTTAAAACGCCTAGGTCACCTTTTGGCAGAAGAACCAGACAAGCTCACAAGGGAAGAATCAACCCTCCTATGTCGCTTTCTCCCTACCCCGCAGTTTGATGAACTCTTCTTTAAACGGAGAAGGGAGAAGAATGCCAGCAAATCCAAAGAACCAAAGGCTTCTATTCCGTTTGTCTATGAAGGAGGTGGAGATAGTCTGCACAATTGCAGGATTTGTGGCAAGAAGGACCACTTTGATGTAGATTGCTCTTACATGAACTTTGTCCCTCAGGGTGTAAATGTTGGCCCCGAGTATATGGTGGTCTGTCGGATATGTGGTCATAGGGTTAGCCAGCCTGTTGGAGATTGTCAGGCATGTGGTACGGAGGGAGGACGTGCTGTTATGAAGGTGTGTATTTTTTGTTTATGCCTTGGTGACCACTTGCCTGAGGACTGCCCACGCACAAAGAGACATAAGACTCGATCCAAATCAGAAGATGCATTGATCATAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

240

Amino Acids

27.1

Weight (kDa)

5.77

Isoelectric Point (pI)

64.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000520)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g08811 FvH4_2g13461
malus_domestica MD02G1305800.v1.1 MD02G1311300.v1.1 MD02G1311400.v1.1 MD03G1254800.v1.1 MD04G1165700.v1.1 MD05G1031200.v1.1 MD06G1150500.v1.1 MD06G1150600.v1.1 MD06G1150900.v1.1 MD06G1151100.v1.1 MD06G1151200.v1.1 MD07G1018900.v1.1 MD07G1019400.v1.1 MD07G1019500.v1.1 MD07G1023400.v1.1 MD08G1234000.v1.1 MD08G1234200.v1.1 MD13G1233200.v1.1 MD17G1268200.v1.1
pyrus_communis pycom02g25630 pycom02g26130 pycom05g02240 pycom05g02610 pycom06g13890 pycom06g13900 pycom07g01500 pycom07g01510 pycom07g01520 pycom07g01530 pycom07g01540 pycom08g17170 pycom08g17230 pycom08g20290 pycom13g20570
rosa_chinensis RchiOBHm_Chr2g0139011 RchiOBHm_Chr3g0492121
rosa_laevigata RLG00000002413 RLG00000019773 RLG00000019781 RLG00000022823 RLG00000024839 RLG00000027358
rosa_multiflora Rmu_sc0000379.1_g000005 Rmu_sc0002029.1_g000002 Rmu_sc0009440.1_g000007
rosa_roxburghii Rroxscaffold_2G00105770 Rroxscaffold_2G00105790 Rroxscaffold_2G00105890 Rroxscaffold_3G00241430 Rroxscaffold_4G00290390 Rroxscaffold_4G00290420 Rroxscaffold_7G00207210
rosa_rugosa Rorug02G0354100 Rorug02G0354200 Rorug02G0354300 Rorug02G0354400 Rorug02G0354500 Rorug03G0323900 Rorug07G0173300 Rorug07G0173400
rosa_samantha Rh1AG336500 Rh2AG403700 Rh2AG403900 Rh2BG414300 Rh2BG414400 Rh2BG474400 Rh2CG389800 Rh2CG390000 Rh2DG423900 Rh2DG424000 Rh2DG483000 Rh3CG333300 Rh3DG335400 Rh6AG108100 Rh6BG103000 Rh6BG103400 Rh6CG096600 Rh6DG090300 Rh7AG315900 Rh7BG306200 Rh7DG314100 Rh7DG314600
rosa_wichuraiana Rw2G032870 Rw7G026680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 110
AatII GACGTC 1 cut(s) 114
Acc36I ACCTGC 1 cut(s) 134
AccI GTMKAC 1 cut(s) 90
AciI CCGC 1 cut(s) 317
AclWI GGATC 1 cut(s) 684
AcyI GRCGYC 1 cut(s) 111
AfaI GTAC 2 cut(s) 191, 589
AjiI CACGTC 1 cut(s) 602
AluBI AGCT 2 cut(s) 271, 720
AluI AGCT 2 cut(s) 271, 720
Alw26I GTCTC 1 cut(s) 670
AlwI GGATC 1 cut(s) 684
Ama87I CYCGRG 1 cut(s) 515
AoxI GGCC 1 cut(s) 511
ApeKI GCWGC 1 cut(s) 48
AspA2I CCTAGG 1 cut(s) 238
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 2 cut(s) 454, 512
AsuC2I CCSGG 1 cut(s) 56
AsuHPI GGTGA 2 cut(s) 236, 653
AvaI CYCGRG 1 cut(s) 515
AvaII GGWCC 1 cut(s) 454
AvrII CCTAGG 1 cut(s) 238
AxyI CCTNAGG 2 cut(s) 495, 654
BbvI GCAGC 1 cut(s) 35
BccI CCATC 1 cut(s) 72
BclI TGATCA 1 cut(s) 711
BcnI CCSGG 1 cut(s) 56
BcoDI GTCTC 1 cut(s) 670
BfaI CTAG 2 cut(s) 216, 239
BfuAI ACCTGC 1 cut(s) 134
BglII AGATCT 1 cut(s) 171
BisI GCNGC 1 cut(s) 49
BlnI CCTAGG 1 cut(s) 238
BlsI GCNGC 1 cut(s) 50
BmcAI AGTACT 1 cut(s) 191
Bme1390I CCNGG 1 cut(s) 56
Bme18I GGWCC 1 cut(s) 454
BmeT110I CYCGRG 1 cut(s) 515
BmgBI CACGTC 1 cut(s) 602
BmgT120I GGNCC 2 cut(s) 454, 512
BmiI GGNNCC 2 cut(s) 180, 514
BmrFI CCNGG 1 cut(s) 56
BmsI GCATC 1 cut(s) 694
BpuMI CCSGG 1 cut(s) 56
BsaBI GATNNNNATC 1 cut(s) 694
BsaHI GRCGYC 1 cut(s) 111
BsaJI CCNNGG 3 cut(s) 143, 238, 637
Bse118I RCCGGY 1 cut(s) 32
Bse1I ACTGG 1 cut(s) 69
Bse21I CCTNAGG 2 cut(s) 495, 654
Bse8I GATNNNNATC 1 cut(s) 694
BseDI CCNNGG 3 cut(s) 143, 238, 637
BseGI GGATG 1 cut(s) 83
BseJI GATNNNNATC 1 cut(s) 694
BseMII CTCAG 2 cut(s) 509, 645
BseNI ACTGG 1 cut(s) 69
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 1 cut(s) 35
BsgI GTGCAG 1 cut(s) 410
BshFI GGCC 1 cut(s) 513
BsiHKCI CYCGRG 1 cut(s) 515
BsiSI CCGG 3 cut(s) 33, 56, 182
BslFI GGGAC 1 cut(s) 476
BsmAI GTCTC 1 cut(s) 670
BsmFI GGGAC 1 cut(s) 476
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 1 cut(s) 513
BsoBI CYCGRG 1 cut(s) 515
Bsp143I GATC 3 cut(s) 171, 689, 711
BspACI CCGC 1 cut(s) 317
BspANI GGCC 1 cut(s) 513
BspCNI CTCAG 2 cut(s) 508, 646
BspLI GGNNCC 2 cut(s) 180, 514
BspMI ACCTGC 1 cut(s) 134
BspPI GGATC 1 cut(s) 684
BsrFI RCCGGY 1 cut(s) 32
BsrI ACTGG 1 cut(s) 69
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 3 cut(s) 143, 238, 637
BssMI GATC 3 cut(s) 171, 689, 711
BssNI GRCGYC 1 cut(s) 111
BssT1I CCWWGG 3 cut(s) 143, 238, 637
Bst4CI ACNGT 1 cut(s) 194
Bst6I CTCTTC 2 cut(s) 219, 338
BstACI GRCGYC 1 cut(s) 111
BstC8I GCNNGC 3 cut(s) 34, 364, 560
BstDEI CTNAG 2 cut(s) 495, 654
BstEII GGTNACC 2 cut(s) 242, 641
BstF5I GGATG 1 cut(s) 83
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 3 cut(s) 174, 692, 714
BstMAI GTCTC 1 cut(s) 670
BstMBI GATC 3 cut(s) 171, 689, 711
BstMWI GCNNNNNNNGC 1 cut(s) 65
BstNSI RCATGY 1 cut(s) 585
BstPI GGTNACC 2 cut(s) 242, 641
BstSCI CCNGG 1 cut(s) 54
BstV1I GCAGC 1 cut(s) 35
BstX2I RGATCY 1 cut(s) 171
BstXI CCANNNNNNTGG 1 cut(s) 566
BstYI RGATCY 1 cut(s) 171
Bsu36I CCTNAGG 2 cut(s) 495, 654
BsuRI GGCC 1 cut(s) 513
BtrI CACGTC 1 cut(s) 602
BtsCI GGATG 1 cut(s) 83
BveI ACCTGC 1 cut(s) 134
Cac8I GCNNGC 3 cut(s) 34, 364, 560
CfoI GCGC 1 cut(s) 61
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 2 cut(s) 454, 512
Csp6I GTAC 2 cut(s) 190, 588
CviAII CATG 2 cut(s) 481, 582
CviQI GTAC 2 cut(s) 190, 588
DdeI CTNAG 2 cut(s) 495, 654
DpnI GATC 3 cut(s) 173, 691, 713
DpnII GATC 3 cut(s) 171, 689, 711
DraI TTTAAA 1 cut(s) 340
DrdI GACNNNNNNGTC 1 cut(s) 110
DseDI GACNNNNNNGTC 1 cut(s) 110
Eam1104I CTCTTC 2 cut(s) 219, 338
EarI CTCTTC 2 cut(s) 219, 338
Eco130I CCWWGG 3 cut(s) 143, 238, 637
Eco47I GGWCC 1 cut(s) 454
Eco81I CCTNAGG 2 cut(s) 495, 654
Eco88I CYCGRG 1 cut(s) 515
Eco91I GGTNACC 2 cut(s) 242, 641
EcoO65I GGTNACC 2 cut(s) 242, 641
EcoT14I CCWWGG 3 cut(s) 143, 238, 637
EcoT22I ATGCAT 1 cut(s) 709
ErhI CCWWGG 3 cut(s) 143, 238, 637
FaeI CATG 2 cut(s) 484, 585
FalI AAGNNNNNCTT 2 cut(s) 443, 475
FaqI GGGAC 1 cut(s) 476
FatI CATG 2 cut(s) 480, 581
FauI CCCGC 1 cut(s) 324
FbaI TGATCA 1 cut(s) 711
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 1 cut(s) 49
FokI GGATG 1 cut(s) 90
Fsp4HI GCNGC 1 cut(s) 49
FspBI CTAG 2 cut(s) 216, 239
GlaI GCGC 1 cut(s) 60
GluI GCNGC 1 cut(s) 49
HaeIII GGCC 1 cut(s) 513
HapII CCGG 3 cut(s) 33, 56, 182
HhaI GCGC 1 cut(s) 61
Hin1I GRCGYC 1 cut(s) 111
Hin1II CATG 2 cut(s) 484, 585
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HinfI GANTC 2 cut(s) 284, 685
HpaII CCGG 3 cut(s) 33, 56, 182
HphI GGTGA 2 cut(s) 236, 653
Hpy166II GTNNAC 1 cut(s) 91
Hpy188I TCNGA 3 cut(s) 116, 537, 700
Hpy8I GTNNAC 1 cut(s) 91
Hpy99I CGWCG 5 cut(s) 92, 104, 107, 164, 167
HpyAV CCTTC 4 cut(s) 342, 401, 445, 607
HpyCH4III ACNGT 1 cut(s) 194
HpyCH4IV ACGT 4 cut(s) 73, 87, 111, 601
HpyCH4V TGCA 4 cut(s) 129, 427, 435, 707
HpyF10VI GCNNNNNNNGC 1 cut(s) 65
HpyF3I CTNAG 2 cut(s) 495, 654
HpySE526I ACGT 4 cut(s) 73, 87, 111, 601
Hsp92I GRCGYC 1 cut(s) 111
Hsp92II CATG 2 cut(s) 484, 585
HspAI GCGC 1 cut(s) 59
KroI GCCGGC 1 cut(s) 32
KroNI GCCGGC 1 cut(s) 34
Ksp22I TGATCA 1 cut(s) 711
Kzo9I GATC 3 cut(s) 171, 689, 711
Lsp1109I GCAGC 1 cut(s) 35
LweI GCATC 1 cut(s) 694
MaeI CTAG 2 cut(s) 216, 239
MaeII ACGT 4 cut(s) 73, 87, 111, 601
MaeIII GTNAC 3 cut(s) 194, 242, 641
MalI GATC 3 cut(s) 173, 691, 713
MboI GATC 3 cut(s) 171, 689, 711
MboII GAAGA 7 cut(s) 218, 236, 269, 293, 325, 367, 713
MfeI CAATTG 1 cut(s) 430
MflI RGATCY 1 cut(s) 171
MluCI AATT 1 cut(s) 430
MlyI GAGTC 1 cut(s) 679
MmeI TCCRAC 2 cut(s) 515, 547
MnlI CCTC 9 cut(s) 34, 160, 222, 302, 404, 504, 586, 590, 649
Mph1103I ATGCAT 1 cut(s) 709
MroNI GCCGGC 1 cut(s) 32
MseI TTAA 2 cut(s) 230, 339
MslI CAYNNNNRTG 2 cut(s) 134, 462
MspI CCGG 3 cut(s) 33, 56, 182
MspR9I CCNGG 1 cut(s) 56
MunI CAATTG 1 cut(s) 430
Mva1269I GAATGC 1 cut(s) 364
MwoI GCNNNNNNNGC 1 cut(s) 65
NaeI GCCGGC 1 cut(s) 34
NciI CCSGG 1 cut(s) 56
NdeII GATC 3 cut(s) 171, 689, 711
NgoMIV GCCGGC 1 cut(s) 32
NlaIII CATG 2 cut(s) 484, 585
NlaIV GGNNCC 2 cut(s) 180, 514
NmuCI GTSAC 2 cut(s) 242, 641
NsiI ATGCAT 1 cut(s) 709
NspI RCATGY 1 cut(s) 585
PcsI WCGNNNNNNNCGW 2 cut(s) 96, 108
PctI GAATGC 1 cut(s) 364
PdiI GCCGGC 1 cut(s) 34
PfeI GAWTC 1 cut(s) 284
PkrI GCNGC 1 cut(s) 50
PleI GAGTC 1 cut(s) 679
PpsI GAGTC 1 cut(s) 679
PspEI GGTNACC 2 cut(s) 242, 641
PspN4I GGNNCC 2 cut(s) 180, 514
PspPI GGNCC 2 cut(s) 454, 512
PsuI RGATCY 1 cut(s) 171
RsaI GTAC 2 cut(s) 191, 589
RsaNI GTAC 2 cut(s) 190, 588
RseI CAYNNNNRTG 2 cut(s) 134, 462
SalI GTCGAC 1 cut(s) 89
SaqAI TTAA 2 cut(s) 230, 339
SatI GCNGC 1 cut(s) 49
Sau3AI GATC 3 cut(s) 171, 689, 711
Sau96I GGNCC 2 cut(s) 454, 512
ScaI AGTACT 1 cut(s) 191
SchI GAGTC 1 cut(s) 679
ScrFI CCNGG 1 cut(s) 56
SfaNI GCATC 1 cut(s) 694
SinI GGWCC 1 cut(s) 454
SmiMI CAYNNNNRTG 2 cut(s) 134, 462
Sse9I AATT 1 cut(s) 430
SsiI CCGC 1 cut(s) 317
SspMI CTAG 2 cut(s) 216, 239
StyD4I CCNGG 1 cut(s) 54
StyI CCWWGG 3 cut(s) 143, 238, 637
TaaI ACNGT 1 cut(s) 194
TaiI ACGT 4 cut(s) 76, 90, 114, 604
TaqI TCGA 2 cut(s) 90, 688
TasI AATT 1 cut(s) 430
TatI WGTACW 1 cut(s) 189
TfiI GAWTC 1 cut(s) 284
Tru1I TTAA 2 cut(s) 230, 339
Tru9I TTAA 2 cut(s) 230, 339
TseFI GTSAC 2 cut(s) 242, 641
TseI GCWGC 1 cut(s) 48
Tsp45I GTSAC 2 cut(s) 242, 641
TspDTI ATGAA 5 cut(s) 237, 342, 420, 497, 626
TspGWI ACGGA 3 cut(s) 358, 384, 605
VpaK11BI GGWCC 1 cut(s) 454
XceI RCATGY 1 cut(s) 585
XmaJI CCTAGG 1 cut(s) 238
XmiI GTMKAC 1 cut(s) 90
XspI CTAG 2 cut(s) 216, 239
ZraI GACGTC 1 cut(s) 112
ZrmI AGTACT 1 cut(s) 191
Zsp2I ATGCAT 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.