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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 517 |
| AciI | CCGC | 1 cut(s) | 721 |
| AclWI | GGATC | 2 cut(s) | 647, 1010 |
| AcsI | RAATTY | 2 cut(s) | 75, 422 |
| AcuI | CTGAAG | 4 cut(s) | 164, 432, 1011, 1041 |
| AfaI | GTAC | 2 cut(s) | 580, 821 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 131, 922 |
| AflII | CTTAAG | 2 cut(s) | 551, 1268 |
| AgsI | TTSAA | 4 cut(s) | 51, 81, 670, 854 |
| AjnI | CCWGG | 2 cut(s) | 34, 919 |
| Alw26I | GTCTC | 5 cut(s) | 244, 345, 489, 653, 1201 |
| AlwI | GGATC | 2 cut(s) | 647, 1010 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1210 |
| Ama87I | CYCGRG | 1 cut(s) | 564 |
| ApeKI | GCWGC | 6 cut(s) | 148, 1154, 1157, 1238, 1241, 1244 |
| ApoI | RAATTY | 2 cut(s) | 75, 422 |
| AspLEI | GCGC | 2 cut(s) | 517, 621 |
| AsuHPI | GGTGA | 4 cut(s) | 52, 329, 613, 820 |
| AvaI | CYCGRG | 1 cut(s) | 564 |
| BanII | GRGCYC | 1 cut(s) | 463 |
| BauI | CACGAG | 2 cut(s) | 486, 510 |
| BbsI | GAAGAC | 2 cut(s) | 338, 1353 |
| BbvI | GCAGC | 6 cut(s) | 160, 1141, 1169, 1225, 1228, 1256 |
| BccI | CCATC | 5 cut(s) | 191, 799, 992, 1064, 1171 |
| BceAI | ACGGC | 1 cut(s) | 708 |
| BciT130I | CCWGG | 2 cut(s) | 36, 921 |
| BciVI | GTATCC | 1 cut(s) | 1096 |
| BclI | TGATCA | 1 cut(s) | 937 |
| BcoDI | GTCTC | 5 cut(s) | 244, 345, 489, 653, 1201 |
| BfaI | CTAG | 4 cut(s) | 636, 663, 1107, 1128 |
| BfmI | CTRYAG | 3 cut(s) | 833, 1155, 1242 |
| BfoI | RGCGCY | 1 cut(s) | 622 |
| BfrI | CTTAAG | 2 cut(s) | 551, 1268 |
| BfuI | GTATCC | 1 cut(s) | 1096 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 710, 770 |
| BglII | AGATCT | 1 cut(s) | 1054 |
| BisI | GCNGC | 6 cut(s) | 149, 1155, 1158, 1239, 1242, 1245 |
| BlpI | GCTNAGC | 1 cut(s) | 615 |
| BlsI | GCNGC | 6 cut(s) | 150, 1156, 1159, 1240, 1243, 1246 |
| Bme1390I | CCNGG | 2 cut(s) | 36, 921 |
| BmeT110I | CYCGRG | 1 cut(s) | 564 |
| BmiI | GGNNCC | 2 cut(s) | 703, 1198 |
| BmrFI | CCNGG | 2 cut(s) | 36, 921 |
| BmrI | ACTGGG | 2 cut(s) | 218, 881 |
| BmsI | GCATC | 6 cut(s) | 427, 496, 550, 751, 986, 1051 |
| BmuI | ACTGGG | 2 cut(s) | 218, 881 |
| BpiI | GAAGAC | 2 cut(s) | 338, 1353 |
| BpmI | CTGGAG | 4 cut(s) | 379, 903, 988, 1324 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 615 |
| BpuEI | CTTGAG | 1 cut(s) | 1049 |
| Bsa29I | ATCGAT | 1 cut(s) | 251 |
| BsaJI | CCNNGG | 3 cut(s) | 35, 920, 1065 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 131, 922 |
| Bse1I | ACTGG | 6 cut(s) | 224, 396, 876, 971, 1215, 1307 |
| Bse3DI | GCAATG | 2 cut(s) | 472, 759 |
| BseBI | CCWGG | 2 cut(s) | 36, 921 |
| BseCI | ATCGAT | 1 cut(s) | 251 |
| BseDI | CCNNGG | 3 cut(s) | 35, 920, 1065 |
| BseGI | GGATG | 5 cut(s) | 167, 511, 732, 1075, 1392 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 131, 922 |
| BseMI | GCAATG | 2 cut(s) | 472, 759 |
| BseMII | CTCAG | 3 cut(s) | 257, 369, 606 |
| BseNI | ACTGG | 6 cut(s) | 224, 396, 876, 971, 1215, 1307 |
| BseXI | GCAGC | 6 cut(s) | 160, 1141, 1169, 1225, 1228, 1256 |
| BsgI | GTGCAG | 1 cut(s) | 345 |
| Bsh1236I | CGCG | 1 cut(s) | 517 |
| BshVI | ATCGAT | 1 cut(s) | 251 |
| BsiHKCI | CYCGRG | 1 cut(s) | 564 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 131, 922 |
| BsmAI | GTCTC | 5 cut(s) | 244, 345, 489, 653, 1201 |
| BsmBI | CGTCTC | 1 cut(s) | 345 |
| BsmI | GAATGC | 1 cut(s) | 849 |
| BsoBI | CYCGRG | 1 cut(s) | 564 |
| Bsp1286I | GDGCHC | 1 cut(s) | 463 |
| Bsp1407I | TGTACA | 1 cut(s) | 819 |
| Bsp143I | GATC | 6 cut(s) | 248, 639, 715, 937, 1015, 1054 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 615 |
| Bsp19I | CCATGG | 1 cut(s) | 1065 |
| BspACI | CCGC | 1 cut(s) | 721 |
| BspCNI | CTCAG | 3 cut(s) | 256, 370, 607 |
| BspDI | ATCGAT | 1 cut(s) | 251 |
| BspFNI | CGCG | 1 cut(s) | 517 |
| BspLI | GGNNCC | 2 cut(s) | 703, 1198 |
| BspMAI | CTGCAG | 3 cut(s) | 837, 1159, 1246 |
| BspPI | GGATC | 2 cut(s) | 647, 1010 |
| BspQI | GCTCTTC | 1 cut(s) | 1017 |
| BspTI | CTTAAG | 2 cut(s) | 551, 1268 |
| BsrDI | GCAATG | 2 cut(s) | 472, 759 |
| BsrGI | TGTACA | 1 cut(s) | 819 |
| BsrI | ACTGG | 6 cut(s) | 224, 396, 876, 971, 1215, 1307 |
| BssECI | CCNNGG | 3 cut(s) | 35, 920, 1065 |
| BssMI | GATC | 6 cut(s) | 248, 639, 715, 937, 1015, 1054 |
| BssSI | CACGAG | 2 cut(s) | 486, 510 |
| BssT1I | CCWWGG | 1 cut(s) | 1065 |
| Bst2BI | CACGAG | 2 cut(s) | 486, 510 |
| Bst2UI | CCWGG | 2 cut(s) | 36, 921 |
| Bst4CI | ACNGT | 4 cut(s) | 9, 122, 238, 596 |
| Bst6I | CTCTTC | 2 cut(s) | 63, 1017 |
| BstAFI | CTTAAG | 2 cut(s) | 551, 1268 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 11 |
| BstAUI | TGTACA | 1 cut(s) | 819 |
| BstC8I | GCNNGC | 3 cut(s) | 16, 463, 1134 |
| BstDEI | CTNAG | 4 cut(s) | 243, 378, 615, 706 |
| BstDSI | CCRYGG | 1 cut(s) | 1065 |
| BstF5I | GGATG | 5 cut(s) | 167, 511, 732, 1075, 1392 |
| BstFNI | CGCG | 1 cut(s) | 517 |
| BstH2I | RGCGCY | 1 cut(s) | 622 |
| BstHHI | GCGC | 2 cut(s) | 517, 621 |
| BstKTI | GATC | 6 cut(s) | 251, 642, 718, 940, 1018, 1057 |
| BstMAI | GTCTC | 5 cut(s) | 244, 345, 489, 653, 1201 |
| BstMBI | GATC | 6 cut(s) | 248, 639, 715, 937, 1015, 1054 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 11, 710, 770, 883, 1154, 1244, 1253 |
| BstNI | CCWGG | 2 cut(s) | 36, 921 |
| BstSCI | CCNGG | 2 cut(s) | 34, 919 |
| BstSFI | CTRYAG | 3 cut(s) | 833, 1155, 1242 |
| BstUI | CGCG | 1 cut(s) | 517 |
| BstV1I | GCAGC | 6 cut(s) | 160, 1141, 1169, 1225, 1228, 1256 |
| BstV2I | GAAGAC | 2 cut(s) | 338, 1353 |
| BstX2I | RGATCY | 2 cut(s) | 639, 1054 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1150, 1170 |
| BstYI | RGATCY | 2 cut(s) | 639, 1054 |
| Bsu15I | ATCGAT | 1 cut(s) | 251 |
| BsuI | GTATCC | 1 cut(s) | 1096 |
| BsuTUI | ATCGAT | 1 cut(s) | 251 |
| BtgI | CCRYGG | 1 cut(s) | 1065 |
| BtsCI | GGATG | 5 cut(s) | 167, 511, 732, 1075, 1392 |
| BtsIMutI | CAGTG | 3 cut(s) | 5, 118, 234 |
| Cac8I | GCNNGC | 3 cut(s) | 16, 463, 1134 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1210 |
| CfoI | GCGC | 2 cut(s) | 517, 621 |
| ClaI | ATCGAT | 1 cut(s) | 251 |
| Csp6I | GTAC | 2 cut(s) | 579, 820 |
| CviAII | CATG | 3 cut(s) | 802, 1066, 1330 |
| CviQI | GTAC | 2 cut(s) | 579, 820 |
| DdeI | CTNAG | 4 cut(s) | 243, 378, 615, 706 |
| DpnI | GATC | 6 cut(s) | 250, 641, 717, 939, 1017, 1056 |
| DpnII | GATC | 6 cut(s) | 248, 639, 715, 937, 1015, 1054 |
| Eam1104I | CTCTTC | 2 cut(s) | 63, 1017 |
| EarI | CTCTTC | 2 cut(s) | 63, 1017 |
| EciI | GGCGGA | 1 cut(s) | 710 |
| Eco130I | CCWWGG | 1 cut(s) | 1065 |
| Eco24I | GRGCYC | 1 cut(s) | 463 |
| Eco57I | CTGAAG | 4 cut(s) | 164, 432, 1011, 1041 |
| Eco88I | CYCGRG | 1 cut(s) | 564 |
| EcoRII | CCWGG | 2 cut(s) | 34, 919 |
| EcoT14I | CCWWGG | 1 cut(s) | 1065 |
| EcoT38I | GRGCYC | 1 cut(s) | 463 |
| ErhI | CCWWGG | 1 cut(s) | 1065 |
| Esp3I | CGTCTC | 1 cut(s) | 345 |
| FaeI | CATG | 3 cut(s) | 805, 1069, 1333 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 780, 812 |
| FatI | CATG | 3 cut(s) | 801, 1065, 1329 |
| FbaI | TGATCA | 1 cut(s) | 937 |
| Fnu4HI | GCNGC | 6 cut(s) | 149, 1155, 1158, 1239, 1242, 1245 |
| FokI | GGATG | 4 cut(s) | 174, 518, 719, 1082 |
| FriOI | GRGCYC | 1 cut(s) | 463 |
| Fsp4HI | GCNGC | 6 cut(s) | 149, 1155, 1158, 1239, 1242, 1245 |
| FspBI | CTAG | 4 cut(s) | 636, 663, 1107, 1128 |
| GlaI | GCGC | 2 cut(s) | 516, 620 |
| GluI | GCNGC | 6 cut(s) | 149, 1155, 1158, 1239, 1242, 1245 |
| GsuI | CTGGAG | 4 cut(s) | 379, 903, 988, 1324 |
| HaeII | RGCGCY | 1 cut(s) | 622 |
| HhaI | GCGC | 2 cut(s) | 517, 621 |
| Hin1II | CATG | 3 cut(s) | 805, 1069, 1333 |
| Hin6I | GCGC | 2 cut(s) | 515, 619 |
| HinP1I | GCGC | 2 cut(s) | 515, 619 |
| HincII | GTYRAC | 1 cut(s) | 368 |
| HindII | GTYRAC | 1 cut(s) | 368 |
| HindIII | AAGCTT | 3 cut(s) | 414, 554, 960 |
| HinfI | GANTC | 2 cut(s) | 334, 1367 |
| HphI | GGTGA | 4 cut(s) | 52, 329, 613, 820 |
| Hpy166II | GTNNAC | 5 cut(s) | 341, 368, 430, 1173, 1311 |
| Hpy188I | TCNGA | 5 cut(s) | 246, 544, 859, 991, 1372 |
| Hpy188III | TCNNGA | 6 cut(s) | 270, 377, 797, 956, 1019, 1058 |
| Hpy8I | GTNNAC | 5 cut(s) | 341, 368, 430, 1173, 1311 |
| HpyAV | CCTTC | 3 cut(s) | 293, 632, 1286 |
| HpyCH4III | ACNGT | 4 cut(s) | 9, 122, 238, 596 |
| HpyCH4IV | ACGT | 3 cut(s) | 364, 748, 1175 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 11, 710, 770, 883, 1154, 1244, 1253 |
| HpyF3I | CTNAG | 4 cut(s) | 243, 378, 615, 706 |
| HpySE526I | ACGT | 3 cut(s) | 364, 748, 1175 |
| Hsp92II | CATG | 3 cut(s) | 805, 1069, 1333 |
| HspAI | GCGC | 2 cut(s) | 515, 619 |
| Ksp22I | TGATCA | 1 cut(s) | 937 |
| Kzo9I | GATC | 6 cut(s) | 248, 639, 715, 937, 1015, 1054 |
| LguI | GCTCTTC | 1 cut(s) | 1017 |
| LmnI | GCTCC | 4 cut(s) | 398, 701, 1165, 1196 |
| Lsp1109I | GCAGC | 6 cut(s) | 160, 1141, 1169, 1225, 1228, 1256 |
| LweI | GCATC | 6 cut(s) | 427, 496, 550, 751, 986, 1051 |
| MaeI | CTAG | 4 cut(s) | 636, 663, 1107, 1128 |
| MaeII | ACGT | 3 cut(s) | 364, 748, 1175 |
| MaeIII | GTNAC | 3 cut(s) | 40, 218, 695 |
| MalI | GATC | 6 cut(s) | 250, 641, 717, 939, 1017, 1056 |
| MboI | GATC | 6 cut(s) | 248, 639, 715, 937, 1015, 1054 |
| MboII | GAAGA | 8 cut(s) | 50, 343, 368, 616, 619, 1034, 1346, 1358 |
| MfeI | CAATTG | 1 cut(s) | 1233 |
| MflI | RGATCY | 2 cut(s) | 639, 1054 |
| MhlI | GDGCHC | 1 cut(s) | 463 |
| MlyI | GAGTC | 1 cut(s) | 328 |
| MnlI | CCTC | 8 cut(s) | 266, 568, 703, 840, 927, 995, 1282, 1299 |
| MseI | TTAA | 6 cut(s) | 407, 552, 644, 909, 1037, 1269 |
| MslI | CAYNNNNRTG | 4 cut(s) | 435, 693, 806, 1168 |
| MspCI | CTTAAG | 2 cut(s) | 551, 1268 |
| MspR9I | CCNGG | 2 cut(s) | 36, 921 |
| MunI | CAATTG | 1 cut(s) | 1233 |
| Mva1269I | GAATGC | 1 cut(s) | 849 |
| MvaI | CCWGG | 2 cut(s) | 36, 921 |
| MvnI | CGCG | 1 cut(s) | 517 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 11, 710, 770, 883, 1154, 1244, 1253 |
| NcoI | CCATGG | 1 cut(s) | 1065 |
| NdeII | GATC | 6 cut(s) | 248, 639, 715, 937, 1015, 1054 |
| NlaIII | CATG | 3 cut(s) | 805, 1069, 1333 |
| NlaIV | GGNNCC | 2 cut(s) | 703, 1198 |
| NmuCI | GTSAC | 2 cut(s) | 40, 695 |
| PciSI | GCTCTTC | 1 cut(s) | 1017 |
| PctI | GAATGC | 1 cut(s) | 849 |
| PfeI | GAWTC | 1 cut(s) | 1367 |
| PkrI | GCNGC | 6 cut(s) | 150, 1156, 1159, 1240, 1243, 1246 |
| PleI | GAGTC | 1 cut(s) | 328 |
| PpsI | GAGTC | 1 cut(s) | 328 |
| Psp6I | CCWGG | 2 cut(s) | 34, 919 |
| PspGI | CCWGG | 2 cut(s) | 34, 919 |
| PspN4I | GGNNCC | 2 cut(s) | 703, 1198 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 268, 300 |
| PstI | CTGCAG | 3 cut(s) | 837, 1159, 1246 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1210 |
| PsuI | RGATCY | 2 cut(s) | 639, 1054 |
| RsaI | GTAC | 2 cut(s) | 580, 821 |
| RsaNI | GTAC | 2 cut(s) | 579, 820 |
| RseI | CAYNNNNRTG | 4 cut(s) | 435, 693, 806, 1168 |
| SapI | GCTCTTC | 1 cut(s) | 1017 |
| SaqAI | TTAA | 6 cut(s) | 407, 552, 644, 909, 1037, 1269 |
| SatI | GCNGC | 6 cut(s) | 149, 1155, 1158, 1239, 1242, 1245 |
| Sau3AI | GATC | 6 cut(s) | 248, 639, 715, 937, 1015, 1054 |
| SchI | GAGTC | 1 cut(s) | 328 |
| ScrFI | CCNGG | 2 cut(s) | 36, 921 |
| SduI | GDGCHC | 1 cut(s) | 463 |
| SfaNI | GCATC | 6 cut(s) | 427, 496, 550, 751, 986, 1051 |
| SfcI | CTRYAG | 3 cut(s) | 833, 1155, 1242 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 435, 693, 806, 1168 |
| SmlI | CTYRAG | 3 cut(s) | 551, 1028, 1268 |
| SmoI | CTYRAG | 3 cut(s) | 551, 1028, 1268 |
| SsiI | CCGC | 1 cut(s) | 721 |
| SspMI | CTAG | 4 cut(s) | 636, 663, 1107, 1128 |
| StyD4I | CCNGG | 2 cut(s) | 34, 919 |
| StyI | CCWWGG | 1 cut(s) | 1065 |
| TaaI | ACNGT | 4 cut(s) | 9, 122, 238, 596 |
| TaiI | ACGT | 3 cut(s) | 367, 751, 1178 |
| TaqI | TCGA | 2 cut(s) | 251, 888 |
| TatI | WGTACW | 1 cut(s) | 819 |
| TfiI | GAWTC | 1 cut(s) | 1367 |
| Tru1I | TTAA | 6 cut(s) | 407, 552, 644, 909, 1037, 1269 |
| Tru9I | TTAA | 6 cut(s) | 407, 552, 644, 909, 1037, 1269 |
| TscAI | CASTG | 3 cut(s) | 12, 125, 241 |
| TseFI | GTSAC | 2 cut(s) | 40, 695 |
| TseI | GCWGC | 6 cut(s) | 148, 1154, 1157, 1238, 1241, 1244 |
| Tsp45I | GTSAC | 2 cut(s) | 40, 695 |
| TspDTI | ATGAA | 6 cut(s) | 50, 375, 468, 614, 1278, 1331 |
| TspGWI | ACGGA | 3 cut(s) | 121, 714, 725 |
| TspRI | CASTG | 3 cut(s) | 12, 125, 241 |
| Vha464I | CTTAAG | 2 cut(s) | 551, 1268 |
| XapI | RAATTY | 2 cut(s) | 75, 422 |
| XspI | CTAG | 4 cut(s) | 636, 663, 1107, 1128 |