MD13G1233200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000520
Physical Location & Seq
Forward (+)
23302941 .. 23304261
1321 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1233200.v1.1.491

Sequence Viewer

Length: 630 bp
ATGATCCGGGTACCCAGAGACGACGCCAAGCGCGGAGGCGGAGGCGGAGGCAGAGGCAGAGGCAGAGGCAGGGCCAATTCGCTCGCGCGATCAGAATCCGGGCGGGGGAAGAATCCAGCCCAGCCAGCGGAGCTCCGAGATGAGAAGAAAGAAACAAAAGCTGTCGAAAGCTCTATGAGTAAACTCCAACTTGATGATGATACTCCTACTTCAAAGAAGAAACGTTACCGCGAGTCTAAGAAATGCCATTCTCAGAATGAGCATTGTGCAGTTTGCCTTGTGGACGGCCACCACCAACATCTGTGTCCCTACCGGGAAAGTGTCCCGTTGGGTGTAACTAAAGTTGGAGAGGGCTATATACTAATGTGTAGGACTTGTGGTTTTGTGGGTAACGTCTGCCTTCATGGCCGCTCTTATGCTCGTCCCTATAGGCGGTGTTATTGGCGTCTGAAACATGGGTACCCAATCCACGAGGAAGTGGAGCAAATCAGGGCAAGACAGAGAGAAATGGCTGAAATGTCCAGGGCACGAGTGGAGAGAGGTGAACCATCCTTATTGGATGATACCTCTTCCGATTCCTCTTCTGATACCTCTTCCGATTCCTCTTCTGATTCCTCTTCTGAGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

210

Amino Acids

23.34

Weight (kDa)

9.13

Isoelectric Point (pI)

52.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000520)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g08811 FvH4_2g13461
malus_domestica MD02G1305800.v1.1 MD02G1311300.v1.1 MD02G1311400.v1.1 MD03G1254800.v1.1 MD04G1165700.v1.1 MD05G1031200.v1.1 MD06G1150500.v1.1 MD06G1150600.v1.1 MD06G1150900.v1.1 MD06G1151100.v1.1 MD06G1151200.v1.1 MD07G1018900.v1.1 MD07G1019400.v1.1 MD07G1019500.v1.1 MD07G1023400.v1.1 MD08G1234000.v1.1 MD08G1234200.v1.1 MD13G1233200.v1.1 MD17G1268200.v1.1
pyrus_communis pycom02g25630 pycom02g26130 pycom05g02240 pycom05g02610 pycom06g13890 pycom06g13900 pycom07g01500 pycom07g01510 pycom07g01520 pycom07g01530 pycom07g01540 pycom08g17170 pycom08g17230 pycom08g20290 pycom13g20570
rosa_chinensis RchiOBHm_Chr2g0139011 RchiOBHm_Chr3g0492121
rosa_laevigata RLG00000002413 RLG00000019773 RLG00000019781 RLG00000022823 RLG00000024839 RLG00000027358
rosa_multiflora Rmu_sc0000379.1_g000005 Rmu_sc0002029.1_g000002 Rmu_sc0009440.1_g000007
rosa_roxburghii Rroxscaffold_2G00105770 Rroxscaffold_2G00105790 Rroxscaffold_2G00105890 Rroxscaffold_3G00241430 Rroxscaffold_4G00290390 Rroxscaffold_4G00290420 Rroxscaffold_7G00207210
rosa_rugosa Rorug02G0354100 Rorug02G0354200 Rorug02G0354300 Rorug02G0354400 Rorug02G0354500 Rorug03G0323900 Rorug07G0173300 Rorug07G0173400
rosa_samantha Rh1AG336500 Rh2AG403700 Rh2AG403900 Rh2BG414300 Rh2BG414400 Rh2BG474400 Rh2CG389800 Rh2CG390000 Rh2DG423900 Rh2DG424000 Rh2DG483000 Rh3CG333300 Rh3DG335400 Rh6AG108100 Rh6BG103000 Rh6BG103400 Rh6CG096600 Rh6DG090300 Rh7AG315900 Rh7BG306200 Rh7DG314100 Rh7DG314600
rosa_wichuraiana Rw2G032870 Rw7G026680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 10, 459
AccB1I GGYRCC 2 cut(s) 10, 459
AccBSI CCGCTC 1 cut(s) 411
AccII CGCG 4 cut(s) 33, 86, 88, 231
AciI CCGC 8 cut(s) 33, 39, 45, 103, 128, 229, 409, 433
AclI AACGTT 1 cut(s) 223
AcoI YGGCCR 2 cut(s) 286, 406
AcyI GRCGYC 2 cut(s) 24, 445
AfaI GTAC 2 cut(s) 12, 461
AfiI CCNNNNNNNGG 3 cut(s) 105, 127, 432
AgsI TTSAA 1 cut(s) 213
AjnI CCWGG 1 cut(s) 521
AluBI AGCT 3 cut(s) 133, 161, 171
AluI AGCT 3 cut(s) 133, 161, 171
Alw21I GWGCWC 1 cut(s) 135
Alw26I GTCTC 1 cut(s) 12
AoxI GGCC 3 cut(s) 72, 286, 406
Asp718I GGTACC 2 cut(s) 10, 459
AspLEI GCGC 2 cut(s) 33, 88
AspS9I GGNCC 1 cut(s) 72
AsuC2I CCSGG 3 cut(s) 8, 100, 314
AsuHPI GGTGA 1 cut(s) 554
BaeGI GKGCMC 1 cut(s) 529
BaeI ACNNNNGTAYC 2 cut(s) 443, 476
BanI GGYRCC 2 cut(s) 10, 459
BanII GRGCYC 1 cut(s) 135
BarI GAAGNNNNNNTAC 2 cut(s) 209, 241
BauI CACGAG 2 cut(s) 470, 528
Bbv12I GWGCWC 1 cut(s) 135
BccI CCATC 1 cut(s) 556
BceAI ACGGC 1 cut(s) 301
BciT130I CCWGG 1 cut(s) 523
BcnI CCSGG 3 cut(s) 8, 100, 314
BcoDI GTCTC 1 cut(s) 12
BfmI CTRYAG 1 cut(s) 427
BglI GCCNNNNNGGC 1 cut(s) 405
BisI GCNGC 1 cut(s) 409
BlsI GCNGC 1 cut(s) 410
Bme1390I CCNGG 4 cut(s) 8, 100, 314, 523
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 2 cut(s) 12, 461
BmrFI CCNGG 4 cut(s) 8, 100, 314, 523
BpuMI CCSGG 3 cut(s) 8, 100, 314
BsaBI GATNNNNATC 1 cut(s) 94
BsaHI GRCGYC 2 cut(s) 24, 445
BsaJI CCNNGG 1 cut(s) 522
Bsc4I CCNNNNNNNGG 3 cut(s) 105, 127, 432
Bse8I GATNNNNATC 1 cut(s) 94
BseBI CCWGG 1 cut(s) 523
BseDI CCNNGG 1 cut(s) 522
BseGI GGATG 2 cut(s) 548, 565
BseJI GATNNNNATC 1 cut(s) 94
BseLI CCNNNNNNNGG 3 cut(s) 105, 127, 432
BseMII CTCAG 2 cut(s) 266, 612
BseSI GKGCMC 1 cut(s) 529
BseYI CCCAGC 1 cut(s) 120
BsgI GTGCAG 1 cut(s) 288
Bsh1236I CGCG 4 cut(s) 33, 86, 88, 231
BshFI GGCC 3 cut(s) 74, 288, 408
BshNI GGYRCC 2 cut(s) 10, 459
BsiHKAI GWGCWC 1 cut(s) 135
BsiSI CCGG 3 cut(s) 7, 99, 313
BslFI GGGAC 3 cut(s) 291, 308, 408
BslI CCNNNNNNNGG 3 cut(s) 105, 127, 432
BsmAI GTCTC 1 cut(s) 12
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 3 cut(s) 291, 308, 408
BsnI GGCC 3 cut(s) 74, 288, 408
Bsp1286I GDGCHC 2 cut(s) 135, 529
Bsp143I GATC 2 cut(s) 3, 89
BspACI CCGC 8 cut(s) 33, 39, 45, 103, 128, 229, 409, 433
BspANI GGCC 3 cut(s) 74, 288, 408
BspCNI CTCAG 2 cut(s) 265, 613
BspFNI CGCG 4 cut(s) 33, 86, 88, 231
BspLI GGNNCC 2 cut(s) 12, 461
BspT107I GGYRCC 2 cut(s) 10, 459
BsrBI CCGCTC 1 cut(s) 411
BssECI CCNNGG 1 cut(s) 522
BssMI GATC 2 cut(s) 3, 89
BssNI GRCGYC 2 cut(s) 24, 445
BssSI CACGAG 2 cut(s) 470, 528
Bst2BI CACGAG 2 cut(s) 470, 528
Bst2UI CCWGG 1 cut(s) 523
Bst6I CTCTTC 5 cut(s) 574, 586, 598, 610, 622
BstACI GRCGYC 2 cut(s) 24, 445
BstC8I GCNNGC 2 cut(s) 84, 126
BstDEI CTNAG 3 cut(s) 237, 252, 621
BstF5I GGATG 2 cut(s) 548, 565
BstFNI CGCG 4 cut(s) 33, 86, 88, 231
BstHHI GCGC 2 cut(s) 33, 88
BstKTI GATC 2 cut(s) 6, 92
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 2 cut(s) 3, 89
BstMWI GCNNNNNNNGC 3 cut(s) 125, 130, 405
BstNI CCWGG 1 cut(s) 523
BstSCI CCNGG 4 cut(s) 6, 98, 312, 521
BstSFI CTRYAG 1 cut(s) 427
BstSLI GKGCMC 1 cut(s) 529
BstUI CGCG 4 cut(s) 33, 86, 88, 231
BsuRI GGCC 3 cut(s) 74, 288, 408
BtsCI GGATG 2 cut(s) 548, 565
Cac8I GCNNGC 2 cut(s) 84, 126
CfoI GCGC 2 cut(s) 33, 88
Cfr13I GGNCC 1 cut(s) 72
CseI GACGC 2 cut(s) 32, 434
Csp6I GTAC 2 cut(s) 11, 460
CviAII CATG 2 cut(s) 404, 455
CviQI GTAC 2 cut(s) 11, 460
DdeI CTNAG 3 cut(s) 237, 252, 621
DpnI GATC 2 cut(s) 5, 91
DpnII GATC 2 cut(s) 3, 89
EaeI YGGCCR 2 cut(s) 286, 406
Eam1104I CTCTTC 5 cut(s) 574, 586, 598, 610, 622
EarI CTCTTC 5 cut(s) 574, 586, 598, 610, 622
EciI GGCGGA 2 cut(s) 54, 60
Ecl136II GAGCTC 1 cut(s) 133
Eco24I GRGCYC 1 cut(s) 135
Eco53kI GAGCTC 1 cut(s) 133
EcoICRI GAGCTC 1 cut(s) 133
EcoRII CCWGG 1 cut(s) 521
EcoT38I GRGCYC 1 cut(s) 135
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 2 cut(s) 407, 458
FaiI YATR 8 cut(s) 176, 357, 359, 405, 417, 429, 456, 628
FaqI GGGAC 3 cut(s) 291, 308, 408
FatI CATG 2 cut(s) 403, 454
FauI CCCGC 1 cut(s) 96
Fnu4HI GCNGC 1 cut(s) 409
FokI GGATG 2 cut(s) 535, 572
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 1 cut(s) 409
GlaI GCGC 2 cut(s) 32, 87
GluI GCNGC 1 cut(s) 409
GsaI CCCAGC 1 cut(s) 124
HaeIII GGCC 3 cut(s) 74, 288, 408
HapII CCGG 3 cut(s) 7, 99, 313
HgaI GACGC 2 cut(s) 32, 434
HhaI GCGC 2 cut(s) 33, 88
Hin1I GRCGYC 2 cut(s) 24, 445
Hin1II CATG 2 cut(s) 407, 458
Hin6I GCGC 2 cut(s) 31, 86
HinP1I GCGC 2 cut(s) 31, 86
HinfI GANTC 6 cut(s) 95, 112, 233, 575, 599, 611
HpaII CCGG 3 cut(s) 7, 99, 313
HphI GGTGA 1 cut(s) 554
Hpy166II GTNNAC 3 cut(s) 182, 283, 545
Hpy188I TCNGA 9 cut(s) 94, 137, 255, 450, 574, 586, 598, 610, 622
Hpy8I GTNNAC 3 cut(s) 182, 283, 545
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 1 cut(s) 410
HpyCH4IV ACGT 2 cut(s) 223, 393
HpyCH4V TGCA 1 cut(s) 269
HpyF10VI GCNNNNNNNGC 3 cut(s) 125, 130, 405
HpyF3I CTNAG 3 cut(s) 237, 252, 621
HpySE526I ACGT 2 cut(s) 223, 393
Hsp92I GRCGYC 2 cut(s) 24, 445
Hsp92II CATG 2 cut(s) 407, 458
HspAI GCGC 2 cut(s) 31, 86
KpnI GGTACC 2 cut(s) 14, 463
Kzo9I GATC 2 cut(s) 3, 89
LmnI GCTCC 3 cut(s) 130, 138, 481
MaeII ACGT 2 cut(s) 223, 393
MaeIII GTNAC 3 cut(s) 224, 334, 389
MalI GATC 2 cut(s) 5, 91
MbiI CCGCTC 1 cut(s) 411
MboI GATC 2 cut(s) 3, 89
MboII GAAGA 8 cut(s) 121, 157, 229, 561, 573, 585, 597, 609
MhlI GDGCHC 2 cut(s) 135, 529
MluCI AATT 1 cut(s) 76
MlyI GAGTC 1 cut(s) 242
MmeI TCCRAC 2 cut(s) 211, 325
MspA1I CMGCKG 1 cut(s) 128
MspI CCGG 3 cut(s) 7, 99, 313
MspR9I CCNGG 4 cut(s) 8, 100, 314, 523
MvaI CCWGG 1 cut(s) 523
MvnI CGCG 4 cut(s) 33, 86, 88, 231
MwoI GCNNNNNNNGC 3 cut(s) 125, 130, 405
NciI CCSGG 3 cut(s) 8, 100, 314
NdeII GATC 2 cut(s) 3, 89
NlaIII CATG 2 cut(s) 407, 458
NlaIV GGNNCC 2 cut(s) 12, 461
PfeI GAWTC 5 cut(s) 95, 112, 575, 599, 611
PkrI GCNGC 1 cut(s) 410
PleI GAGTC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 241
Psp124BI GAGCTC 1 cut(s) 135
Psp1406I AACGTT 1 cut(s) 223
Psp6I CCWGG 1 cut(s) 521
PspFI CCCAGC 1 cut(s) 120
PspGI CCWGG 1 cut(s) 521
PspN4I GGNNCC 2 cut(s) 12, 461
PspPI GGNCC 1 cut(s) 72
RsaI GTAC 2 cut(s) 12, 461
RsaNI GTAC 2 cut(s) 11, 460
SacI GAGCTC 1 cut(s) 135
SatI GCNGC 1 cut(s) 409
Sau3AI GATC 2 cut(s) 3, 89
Sau96I GGNCC 1 cut(s) 72
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 4 cut(s) 8, 100, 314, 523
SduI GDGCHC 2 cut(s) 135, 529
SetI ASST 8 cut(s) 135, 163, 173, 226, 396, 544, 569, 593
SfcI CTRYAG 1 cut(s) 427
Sse9I AATT 1 cut(s) 76
SsiI CCGC 8 cut(s) 33, 39, 45, 103, 128, 229, 409, 433
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 4 cut(s) 6, 98, 312, 521
TaiI ACGT 2 cut(s) 226, 396
TaqI TCGA 1 cut(s) 165
TasI AATT 1 cut(s) 76
TauI GCSGC 1 cut(s) 411
TfiI GAWTC 5 cut(s) 95, 112, 575, 599, 611
TspDTI ATGAA 1 cut(s) 392
XcmI CCANNNNNNNNNTGG 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.