MD06G1151200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0000520
Physical Location & Seq
Forward (+)
29454186 .. 29460923
6738 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1151200.v1.1.491

Sequence Viewer

Length: 552 bp
ATGAAACGCCGATTCGAAGGCGGACCAGGAGGATCCACGGAGGGGGAGGGTCCAATTCCGACGCAACCCACGAAGCTCCGAGGTGAAATAGATGCATATAAAAATAAAGAAAAAGAGTATGTTAAAGACTCTGTAATGACTCATCTCACAAAAGTAGGGGCTGCTCAAGACTCTCAGACTCAACACCAACTTGAGGAGAAGAATACTCCTCAAGGTTGGCTCGAGGAGGACATACTTTCCAAAGGCCCACCCATATGCATAATTGCTAGGCCTCCTGAATTTCCGTCTAGTTGTGCAATTTGCACGAAGCCTGGCCACTGGCCGAATCAATGCCCCTGGAGGAGACAGGTCCCCTATGGCATAACTCAGGTTGGTAAGGGCTATGTAGTAGATGGGCGGCGTGCTATTCGCCTTAACTGTGCTTATTGTCATGAGACGGGTACTCACAGGGTTCATGACTGCCCAGAATGGAACAAACTACTCATTGAAACTGGAGGGGATGTGCCCTTGCACCCATGTTGGTTCGATGAAAAACCACCTGCTGATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

184

Amino Acids

20.5

Weight (kDa)

6.65

Isoelectric Point (pI)

52.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000520)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g08811 FvH4_2g13461
malus_domestica MD02G1305800.v1.1 MD02G1311300.v1.1 MD02G1311400.v1.1 MD03G1254800.v1.1 MD04G1165700.v1.1 MD05G1031200.v1.1 MD06G1150500.v1.1 MD06G1150600.v1.1 MD06G1150900.v1.1 MD06G1151100.v1.1 MD06G1151200.v1.1 MD07G1018900.v1.1 MD07G1019400.v1.1 MD07G1019500.v1.1 MD07G1023400.v1.1 MD08G1234000.v1.1 MD08G1234200.v1.1 MD13G1233200.v1.1 MD17G1268200.v1.1
pyrus_communis pycom02g25630 pycom02g26130 pycom05g02240 pycom05g02610 pycom06g13890 pycom06g13900 pycom07g01500 pycom07g01510 pycom07g01520 pycom07g01530 pycom07g01540 pycom08g17170 pycom08g17230 pycom08g20290 pycom13g20570
rosa_chinensis RchiOBHm_Chr2g0139011 RchiOBHm_Chr3g0492121
rosa_laevigata RLG00000002413 RLG00000019773 RLG00000019781 RLG00000022823 RLG00000024839 RLG00000027358
rosa_multiflora Rmu_sc0000379.1_g000005 Rmu_sc0002029.1_g000002 Rmu_sc0009440.1_g000007
rosa_roxburghii Rroxscaffold_2G00105770 Rroxscaffold_2G00105790 Rroxscaffold_2G00105890 Rroxscaffold_3G00241430 Rroxscaffold_4G00290390 Rroxscaffold_4G00290420 Rroxscaffold_7G00207210
rosa_rugosa Rorug02G0354100 Rorug02G0354200 Rorug02G0354300 Rorug02G0354400 Rorug02G0354500 Rorug03G0323900 Rorug07G0173300 Rorug07G0173400
rosa_samantha Rh1AG336500 Rh2AG403700 Rh2AG403900 Rh2BG414300 Rh2BG414400 Rh2BG474400 Rh2CG389800 Rh2CG390000 Rh2DG423900 Rh2DG424000 Rh2DG483000 Rh3CG333300 Rh3DG335400 Rh6AG108100 Rh6BG103000 Rh6BG103400 Rh6CG096600 Rh6DG090300 Rh7AG315900 Rh7BG306200 Rh7DG314100 Rh7DG314600
rosa_wichuraiana Rw2G032870 Rw7G026680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 547
Acc36I ACCTGC 1 cut(s) 547
AciI CCGC 2 cut(s) 21, 397
AclWI GGATC 2 cut(s) 27, 40
AcoI YGGCCR 2 cut(s) 313, 320
AcsI RAATTY 1 cut(s) 278
AfaI GTAC 1 cut(s) 442
AfiI CCNNNNNNNGG 2 cut(s) 42, 193
AgsI TTSAA 1 cut(s) 488
AjnI CCWGG 3 cut(s) 25, 310, 335
AluBI AGCT 1 cut(s) 76
AluI AGCT 1 cut(s) 76
Alw26I GTCTC 2 cut(s) 337, 428
AlwI GGATC 2 cut(s) 27, 40
Ama87I CYCGRG 1 cut(s) 221
AoxI GGCC 4 cut(s) 244, 269, 313, 320
ApeKI GCWGC 1 cut(s) 161
ApoI RAATTY 1 cut(s) 278
AspS9I GGNCC 4 cut(s) 23, 50, 245, 349
AsuHPI GGTGA 1 cut(s) 95
AsuII TTCGAA 1 cut(s) 15
AvaI CYCGRG 1 cut(s) 221
AvaII GGWCC 3 cut(s) 23, 50, 349
BaeGI GKGCMC 1 cut(s) 507
BalI TGGCCA 1 cut(s) 315
BamHI GGATCC 1 cut(s) 32
BbvI GCAGC 1 cut(s) 148
BccI CCATC 1 cut(s) 386
BciT130I CCWGG 3 cut(s) 27, 312, 337
BcoDI GTCTC 2 cut(s) 337, 428
BfaI CTAG 2 cut(s) 267, 288
BfuAI ACCTGC 1 cut(s) 547
BisI GCNGC 2 cut(s) 162, 398
BlsI GCNGC 2 cut(s) 163, 399
Bme1390I CCNGG 3 cut(s) 27, 312, 337
Bme18I GGWCC 3 cut(s) 23, 50, 349
BmeT110I CYCGRG 1 cut(s) 221
BmgT120I GGNCC 4 cut(s) 23, 50, 245, 349
BmiI GGNNCC 3 cut(s) 34, 51, 351
BmrFI CCNGG 3 cut(s) 27, 312, 337
BmsI GCATC 1 cut(s) 82
BpmI CTGGAG 2 cut(s) 358, 513
Bpu14I TTCGAA 1 cut(s) 15
BpuEI CTTGAG 3 cut(s) 150, 195, 212
BsaJI CCNNGG 3 cut(s) 36, 79, 335
BsaXI ACNNNNNCTCC 2 cut(s) 486, 516
Bsc4I CCNNNNNNNGG 2 cut(s) 42, 193
Bse1I ACTGG 2 cut(s) 323, 496
BseBI CCWGG 3 cut(s) 27, 312, 337
BseDI CCNNGG 3 cut(s) 36, 79, 335
BseGI GGATG 1 cut(s) 505
BseLI CCNNNNNNNGG 2 cut(s) 42, 193
BseMII CTCAG 2 cut(s) 188, 380
BseNI ACTGG 2 cut(s) 323, 496
BseRI GAGGAG 4 cut(s) 198, 209, 239, 355
BseSI GKGCMC 1 cut(s) 507
BseXI GCAGC 1 cut(s) 148
BshFI GGCC 4 cut(s) 246, 271, 315, 322
BsiHKCI CYCGRG 1 cut(s) 221
BslFI GGGAC 1 cut(s) 335
BslI CCNNNNNNNGG 2 cut(s) 42, 193
BsmAI GTCTC 2 cut(s) 337, 428
BsmBI CGTCTC 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 335
BsnI GGCC 4 cut(s) 246, 271, 315, 322
BsoBI CYCGRG 1 cut(s) 221
Bsp119I TTCGAA 1 cut(s) 15
Bsp1286I GDGCHC 1 cut(s) 507
Bsp143I GATC 1 cut(s) 32
BspACI CCGC 2 cut(s) 21, 397
BspANI GGCC 4 cut(s) 246, 271, 315, 322
BspCNI CTCAG 2 cut(s) 187, 379
BspHI TCATGA 2 cut(s) 430, 454
BspLI GGNNCC 3 cut(s) 34, 51, 351
BspMI ACCTGC 1 cut(s) 547
BspPI GGATC 2 cut(s) 27, 40
BspT104I TTCGAA 1 cut(s) 15
BsrI ACTGG 2 cut(s) 323, 496
BssECI CCNNGG 3 cut(s) 36, 79, 335
BssMI GATC 1 cut(s) 32
Bst2UI CCWGG 3 cut(s) 27, 312, 337
Bst4CI ACNGT 1 cut(s) 419
BstBI TTCGAA 1 cut(s) 15
BstC8I GCNNGC 1 cut(s) 402
BstDEI CTNAG 2 cut(s) 174, 366
BstDSI CCRYGG 1 cut(s) 36
BstF5I GGATG 1 cut(s) 505
BstKTI GATC 1 cut(s) 35
BstMAI GTCTC 2 cut(s) 337, 428
BstMBI GATC 1 cut(s) 32
BstNI CCWGG 3 cut(s) 27, 312, 337
BstSCI CCNGG 3 cut(s) 25, 310, 335
BstSLI GKGCMC 1 cut(s) 507
BstV1I GCAGC 1 cut(s) 148
BstX2I RGATCY 1 cut(s) 32
BstYI RGATCY 1 cut(s) 32
BsuRI GGCC 4 cut(s) 246, 271, 315, 322
BtgI CCRYGG 1 cut(s) 36
BtsCI GGATG 1 cut(s) 505
BtsIMutI CAGTG 1 cut(s) 316
BveI ACCTGC 1 cut(s) 547
Cac8I GCNNGC 1 cut(s) 402
CciI TCATGA 2 cut(s) 430, 454
Cfr13I GGNCC 4 cut(s) 23, 50, 245, 349
CseI GACGC 1 cut(s) 70
Csp6I GTAC 1 cut(s) 441
CviAII CATG 3 cut(s) 431, 455, 516
CviJI RGCY 9 cut(s) 76, 161, 220, 246, 271, 310, 315, 322, 381
CviKI_1 RGCY 9 cut(s) 76, 161, 220, 246, 271, 310, 315, 322, 381
CviQI GTAC 1 cut(s) 441
DdeI CTNAG 2 cut(s) 174, 366
DpnI GATC 1 cut(s) 34
DpnII GATC 1 cut(s) 32
EaeI YGGCCR 2 cut(s) 313, 320
EciI GGCGGA 1 cut(s) 36
Eco147I AGGCCT 1 cut(s) 271
Eco47I GGWCC 3 cut(s) 23, 50, 349
Eco88I CYCGRG 1 cut(s) 221
EcoO109I RGGNCCY 1 cut(s) 349
EcoRII CCWGG 3 cut(s) 25, 310, 335
EcoT22I ATGCAT 2 cut(s) 97, 260
Esp3I CGTCTC 1 cut(s) 428
FaeI CATG 3 cut(s) 434, 458, 519
FaqI GGGAC 1 cut(s) 335
FatI CATG 3 cut(s) 430, 454, 515
FauNDI CATATG 1 cut(s) 254
Fnu4HI GCNGC 2 cut(s) 162, 398
FokI GGATG 1 cut(s) 512
Fsp4HI GCNGC 2 cut(s) 162, 398
FspBI CTAG 2 cut(s) 267, 288
GluI GCNGC 2 cut(s) 162, 398
GsuI CTGGAG 2 cut(s) 358, 513
HaeIII GGCC 4 cut(s) 246, 271, 315, 322
HgaI GACGC 1 cut(s) 70
Hin1II CATG 3 cut(s) 434, 458, 519
HinfI GANTC 6 cut(s) 12, 128, 139, 170, 178, 325
HphI GGTGA 1 cut(s) 95
Hpy188I TCNGA 3 cut(s) 60, 80, 177
Hpy188III TCNNGA 4 cut(s) 167, 275, 431, 455
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 1 cut(s) 11
HpyCH4III ACNGT 1 cut(s) 419
HpyCH4V TGCA 5 cut(s) 95, 258, 296, 303, 511
HpyF3I CTNAG 2 cut(s) 174, 366
Hsp92II CATG 3 cut(s) 434, 458, 519
Kzo9I GATC 1 cut(s) 32
LmnI GCTCC 1 cut(s) 81
Lsp1109I GCAGC 1 cut(s) 148
LweI GCATC 1 cut(s) 82
MaeI CTAG 2 cut(s) 267, 288
MalI GATC 1 cut(s) 34
MboI GATC 1 cut(s) 32
MboII GAAGA 1 cut(s) 211
MflI RGATCY 1 cut(s) 32
MhlI GDGCHC 1 cut(s) 507
MlsI TGGCCA 1 cut(s) 315
MluCI AATT 4 cut(s) 54, 261, 278, 297
MluNI TGGCCA 1 cut(s) 315
MlyI GAGTC 4 cut(s) 122, 133, 164, 172
MmeI TCCRAC 1 cut(s) 83
Mox20I TGGCCA 1 cut(s) 315
Mph1103I ATGCAT 2 cut(s) 97, 260
MscI TGGCCA 1 cut(s) 315
MseI TTAA 2 cut(s) 123, 414
MslI CAYNNNNRTG 1 cut(s) 253
Msp20I TGGCCA 1 cut(s) 315
MspR9I CCNGG 3 cut(s) 27, 312, 337
MvaI CCWGG 3 cut(s) 27, 312, 337
NdeI CATATG 1 cut(s) 254
NdeII GATC 1 cut(s) 32
NlaIII CATG 3 cut(s) 434, 458, 519
NlaIV GGNNCC 3 cut(s) 34, 51, 351
NsiI ATGCAT 2 cut(s) 97, 260
NspV TTCGAA 1 cut(s) 15
PaeR7I CTCGAG 1 cut(s) 221
PagI TCATGA 2 cut(s) 430, 454
PaqCI CACCTGC 1 cut(s) 547
PceI AGGCCT 1 cut(s) 271
PcsI WCGNNNNNNNCGW 1 cut(s) 68
PfeI GAWTC 2 cut(s) 12, 325
PkrI GCNGC 2 cut(s) 163, 399
PleI GAGTC 4 cut(s) 122, 133, 164, 172
PpsI GAGTC 4 cut(s) 122, 133, 164, 172
PpuMI RGGWCCY 1 cut(s) 349
Psp5II RGGWCCY 1 cut(s) 349
Psp6I CCWGG 3 cut(s) 25, 310, 335
PspGI CCWGG 3 cut(s) 25, 310, 335
PspN4I GGNNCC 3 cut(s) 34, 51, 351
PspPI GGNCC 4 cut(s) 23, 50, 245, 349
PspPPI RGGWCCY 1 cut(s) 349
PspXI VCTCGAGB 1 cut(s) 221
PsuI RGATCY 1 cut(s) 32
RsaI GTAC 1 cut(s) 442
RsaNI GTAC 1 cut(s) 441
RseI CAYNNNNRTG 1 cut(s) 253
SaqAI TTAA 2 cut(s) 123, 414
SatI GCNGC 2 cut(s) 162, 398
Sau3AI GATC 1 cut(s) 32
Sau96I GGNCC 4 cut(s) 23, 50, 245, 349
SchI GAGTC 4 cut(s) 122, 133, 164, 172
ScrFI CCNGG 3 cut(s) 27, 312, 337
SduI GDGCHC 1 cut(s) 507
SetI ASST 6 cut(s) 78, 85, 217, 351, 372, 541
SfaNI GCATC 1 cut(s) 82
Sfr274I CTCGAG 1 cut(s) 221
SfuI TTCGAA 1 cut(s) 15
SinI GGWCC 3 cut(s) 23, 50, 349
SlaI CTCGAG 1 cut(s) 221
SmiMI CAYNNNNRTG 1 cut(s) 253
SmlI CTYRAG 4 cut(s) 165, 191, 210, 221
SmoI CTYRAG 4 cut(s) 165, 191, 210, 221
Sse9I AATT 4 cut(s) 54, 261, 278, 297
SseBI AGGCCT 1 cut(s) 271
SsiI CCGC 2 cut(s) 21, 397
SspMI CTAG 2 cut(s) 267, 288
StuI AGGCCT 1 cut(s) 271
StyD4I CCNGG 3 cut(s) 25, 310, 335
TaaI ACNGT 1 cut(s) 419
TaqI TCGA 3 cut(s) 15, 222, 525
TasI AATT 4 cut(s) 54, 261, 278, 297
TauI GCSGC 1 cut(s) 400
TfiI GAWTC 2 cut(s) 12, 325
Tru1I TTAA 2 cut(s) 123, 414
Tru9I TTAA 2 cut(s) 123, 414
TscAI CASTG 1 cut(s) 323
TseI GCWGC 1 cut(s) 161
TspDTI ATGAA 3 cut(s) 17, 443, 543
TspGWI ACGGA 2 cut(s) 53, 273
TspRI CASTG 1 cut(s) 323
VpaK11BI GGWCC 3 cut(s) 23, 50, 349
XapI RAATTY 1 cut(s) 278
XhoI CTCGAG 1 cut(s) 221
XspI CTAG 2 cut(s) 267, 288
Zsp2I ATGCAT 2 cut(s) 97, 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.