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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 75 |
| AciI | CCGC | 2 cut(s) | 75, 306 |
| ApeKI | GCWGC | 2 cut(s) | 70, 248 |
| AspLEI | GCGC | 1 cut(s) | 75 |
| AsuHPI | GGTGA | 1 cut(s) | 271 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 204, 237 |
| BbvI | GCAGC | 2 cut(s) | 82, 235 |
| BccI | CCATC | 1 cut(s) | 58 |
| BfmI | CTRYAG | 1 cut(s) | 249 |
| BglII | AGATCT | 3 cut(s) | 57, 158, 300 |
| BisI | GCNGC | 3 cut(s) | 71, 76, 249 |
| BlsI | GCNGC | 3 cut(s) | 72, 77, 250 |
| BmsI | GCATC | 1 cut(s) | 198 |
| BpmI | CTGGAG | 1 cut(s) | 237 |
| BsaJI | CCNNGG | 1 cut(s) | 102 |
| Bse1I | ACTGG | 1 cut(s) | 220 |
| BseDI | CCNNGG | 1 cut(s) | 102 |
| BseGI | GGATG | 1 cut(s) | 299 |
| BseMII | CTCAG | 1 cut(s) | 30 |
| BseNI | ACTGG | 1 cut(s) | 220 |
| BseRI | GAGGAG | 2 cut(s) | 54, 303 |
| BseXI | GCAGC | 2 cut(s) | 82, 235 |
| Bsh1236I | CGCG | 1 cut(s) | 75 |
| Bsp143I | GATC | 3 cut(s) | 57, 158, 300 |
| BspACI | CCGC | 2 cut(s) | 75, 306 |
| BspCNI | CTCAG | 1 cut(s) | 29 |
| BspFNI | CGCG | 1 cut(s) | 75 |
| BspHI | TCATGA | 2 cut(s) | 310, 317 |
| BspMAI | CTGCAG | 1 cut(s) | 253 |
| BsrI | ACTGG | 1 cut(s) | 220 |
| BssECI | CCNNGG | 1 cut(s) | 102 |
| BssMI | GATC | 3 cut(s) | 57, 158, 300 |
| Bst6I | CTCTTC | 3 cut(s) | 130, 136, 141 |
| BstC8I | GCNNGC | 1 cut(s) | 187 |
| BstDEI | CTNAG | 1 cut(s) | 16 |
| BstF5I | GGATG | 1 cut(s) | 299 |
| BstFNI | CGCG | 1 cut(s) | 75 |
| BstHHI | GCGC | 1 cut(s) | 75 |
| BstKTI | GATC | 3 cut(s) | 60, 161, 303 |
| BstMBI | GATC | 3 cut(s) | 57, 158, 300 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 254 |
| BstSFI | CTRYAG | 1 cut(s) | 249 |
| BstUI | CGCG | 1 cut(s) | 75 |
| BstV1I | GCAGC | 2 cut(s) | 82, 235 |
| BstX2I | RGATCY | 3 cut(s) | 57, 158, 300 |
| BstYI | RGATCY | 3 cut(s) | 57, 158, 300 |
| BtsCI | GGATG | 1 cut(s) | 299 |
| Cac8I | GCNNGC | 1 cut(s) | 187 |
| CciI | TCATGA | 2 cut(s) | 310, 317 |
| CfoI | GCGC | 1 cut(s) | 75 |
| CseI | GACGC | 1 cut(s) | 33 |
| CviAII | CATG | 2 cut(s) | 311, 318 |
| CviJI | RGCY | 3 cut(s) | 91, 212, 248 |
| CviKI_1 | RGCY | 3 cut(s) | 91, 212, 248 |
| DdeI | CTNAG | 1 cut(s) | 16 |
| DpnI | GATC | 3 cut(s) | 59, 160, 302 |
| DpnII | GATC | 3 cut(s) | 57, 158, 300 |
| Eam1104I | CTCTTC | 3 cut(s) | 130, 136, 141 |
| EarI | CTCTTC | 3 cut(s) | 130, 136, 141 |
| FaeI | CATG | 2 cut(s) | 314, 321 |
| FaiI | YATR | 3 cut(s) | 110, 312, 319 |
| FatI | CATG | 2 cut(s) | 310, 317 |
| Fnu4HI | GCNGC | 3 cut(s) | 71, 76, 249 |
| FokI | GGATG | 1 cut(s) | 306 |
| Fsp4HI | GCNGC | 3 cut(s) | 71, 76, 249 |
| GlaI | GCGC | 1 cut(s) | 74 |
| GluI | GCNGC | 3 cut(s) | 71, 76, 249 |
| GsuI | CTGGAG | 1 cut(s) | 237 |
| HgaI | GACGC | 1 cut(s) | 33 |
| HhaI | GCGC | 1 cut(s) | 75 |
| Hin1II | CATG | 2 cut(s) | 314, 321 |
| Hin6I | GCGC | 1 cut(s) | 73 |
| HinP1I | GCGC | 1 cut(s) | 73 |
| HinfI | GANTC | 1 cut(s) | 314 |
| HphI | GGTGA | 1 cut(s) | 271 |
| Hpy188I | TCNGA | 5 cut(s) | 49, 103, 150, 226, 300 |
| Hpy188III | TCNNGA | 3 cut(s) | 61, 311, 318 |
| Hpy99I | CGWCG | 1 cut(s) | 49 |
| HpyAV | CCTTC | 1 cut(s) | 129 |
| HpyCH4V | TGCA | 2 cut(s) | 29, 251 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 254 |
| HpyF3I | CTNAG | 1 cut(s) | 16 |
| Hsp92II | CATG | 2 cut(s) | 314, 321 |
| HspAI | GCGC | 1 cut(s) | 73 |
| Kzo9I | GATC | 3 cut(s) | 57, 158, 300 |
| LmnI | GCTCC | 1 cut(s) | 41 |
| LpnPI | CCDG | 4 cut(s) | 3, 171, 177, 201 |
| Lsp1109I | GCAGC | 2 cut(s) | 82, 235 |
| LweI | GCATC | 1 cut(s) | 198 |
| MaeIII | GTNAC | 1 cut(s) | 277 |
| MalI | GATC | 3 cut(s) | 59, 160, 302 |
| MboI | GATC | 3 cut(s) | 57, 158, 300 |
| MboII | GAAGA | 5 cut(s) | 117, 123, 128, 252, 255 |
| MflI | RGATCY | 3 cut(s) | 57, 158, 300 |
| MluCI | AATT | 3 cut(s) | 10, 32, 179 |
| MmeI | TCCRAC | 1 cut(s) | 27 |
| MnlI | CCTC | 7 cut(s) | 32, 97, 133, 139, 185, 281, 284 |
| MseI | TTAA | 1 cut(s) | 144 |
| MvnI | CGCG | 1 cut(s) | 75 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 254 |
| NdeII | GATC | 3 cut(s) | 57, 158, 300 |
| NlaIII | CATG | 2 cut(s) | 314, 321 |
| NmuCI | GTSAC | 1 cut(s) | 277 |
| PagI | TCATGA | 2 cut(s) | 310, 317 |
| PfeI | GAWTC | 1 cut(s) | 314 |
| PkrI | GCNGC | 3 cut(s) | 72, 77, 250 |
| PstI | CTGCAG | 1 cut(s) | 253 |
| PsuI | RGATCY | 3 cut(s) | 57, 158, 300 |
| SaqAI | TTAA | 1 cut(s) | 144 |
| SatI | GCNGC | 3 cut(s) | 71, 76, 249 |
| Sau3AI | GATC | 3 cut(s) | 57, 158, 300 |
| SetI | ASST | 1 cut(s) | 177 |
| SfaNI | GCATC | 1 cut(s) | 198 |
| SfcI | CTRYAG | 1 cut(s) | 249 |
| SgeI | CNNG | 8 cut(s) | 30, 73, 86, 115, 176, 198, 205, 228 |
| Sse9I | AATT | 3 cut(s) | 10, 32, 179 |
| SsiI | CCGC | 2 cut(s) | 75, 306 |
| TaqI | TCGA | 1 cut(s) | 62 |
| TasI | AATT | 3 cut(s) | 10, 32, 179 |
| TauI | GCSGC | 1 cut(s) | 78 |
| TfiI | GAWTC | 1 cut(s) | 314 |
| Tru1I | TTAA | 1 cut(s) | 144 |
| Tru9I | TTAA | 1 cut(s) | 144 |
| TseFI | GTSAC | 1 cut(s) | 277 |
| TseI | GCWGC | 2 cut(s) | 70, 248 |
| Tsp45I | GTSAC | 1 cut(s) | 277 |
| TspDTI | ATGAA | 2 cut(s) | 101, 125 |