pycom02g25630

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000520
Physical Location & Seq
Reverse (-)
23476005 .. 23477680
1676 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g25630.2

Sequence Viewer

Length: 639 bp
ATGCAGAAGCCCCAAATTCTCTCGTCTCTTGAAAACAAAACCATGAACCCCCGATCCAGGGCGATAGCCAAGGCCAAGGCCAAGGCTAAGGCCAAGTCCAAGTCCAAGTCCCTAGCCGGACCAGGATCTGGATCCGGGCAGGAGAAGATTCCAGGCCAACGGACGAAGCTCGGAGGTGATCGATCCGGGCTCCTCCCTCTTCTCTTGGTGTGCTTTTTCGCATATGGCCCTCCCTCTCAAGTCAAAAATGAAAAGGCTGTTGAAGAGAATCTGGCTAAACTCCAACTTGACGAGGGGAATACTACTACTCCAAAATCAAAACCAGAAGAGAAGAAAATGAGCAGGGCATTCACAGCTGAGAAACCTTTCAGATTCCAATTCAAACACCCTTGTGAAGTTTGCAATGTGTTTGGCCACTGGAGTGATTCATGCCCCTACCTGAAACGTATACCAGACAATGTAACTGAGGTTGGCAAGGGCTACATAATATTGGATGGCCGTGGGCTGAGGTATGCTGATATGATGTTCTGTCTTCTTTGTGGCAAATTTTGTGACCACGAGGATGAGGACTGCCCGGATTTAAGCAAATTCATTGCAGAAGGCCACCCCCTTCTCAACCTAGTTCCTCGCAGCCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

213

Amino Acids

23.44

Weight (kDa)

9.22

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000520)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g08811 FvH4_2g13461
malus_domestica MD02G1305800.v1.1 MD02G1311300.v1.1 MD02G1311400.v1.1 MD03G1254800.v1.1 MD04G1165700.v1.1 MD05G1031200.v1.1 MD06G1150500.v1.1 MD06G1150600.v1.1 MD06G1150900.v1.1 MD06G1151100.v1.1 MD06G1151200.v1.1 MD07G1018900.v1.1 MD07G1019400.v1.1 MD07G1019500.v1.1 MD07G1023400.v1.1 MD08G1234000.v1.1 MD08G1234200.v1.1 MD13G1233200.v1.1 MD17G1268200.v1.1
pyrus_communis pycom02g25630 pycom02g26130 pycom05g02240 pycom05g02610 pycom06g13890 pycom06g13900 pycom07g01500 pycom07g01510 pycom07g01520 pycom07g01530 pycom07g01540 pycom08g17170 pycom08g17230 pycom08g20290 pycom13g20570
rosa_chinensis RchiOBHm_Chr2g0139011 RchiOBHm_Chr3g0492121
rosa_laevigata RLG00000002413 RLG00000019773 RLG00000019781 RLG00000022823 RLG00000024839 RLG00000027358
rosa_multiflora Rmu_sc0000379.1_g000005 Rmu_sc0002029.1_g000002 Rmu_sc0009440.1_g000007
rosa_roxburghii Rroxscaffold_2G00105770 Rroxscaffold_2G00105790 Rroxscaffold_2G00105890 Rroxscaffold_3G00241430 Rroxscaffold_4G00290390 Rroxscaffold_4G00290420 Rroxscaffold_7G00207210
rosa_rugosa Rorug02G0354100 Rorug02G0354200 Rorug02G0354300 Rorug02G0354400 Rorug02G0354500 Rorug03G0323900 Rorug07G0173300 Rorug07G0173400
rosa_samantha Rh1AG336500 Rh2AG403700 Rh2AG403900 Rh2BG414300 Rh2BG414400 Rh2BG474400 Rh2CG389800 Rh2CG390000 Rh2DG423900 Rh2DG424000 Rh2DG483000 Rh3CG333300 Rh3DG335400 Rh6AG108100 Rh6BG103000 Rh6BG103400 Rh6CG096600 Rh6DG090300 Rh7AG315900 Rh7BG306200 Rh7DG314100 Rh7DG314600
rosa_wichuraiana Rw2G032870 Rw7G026680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 128
AccI GTMKAC 1 cut(s) 448
AclWI GGATC 5 cut(s) 48, 126, 133, 139, 177
AcoI YGGCCR 2 cut(s) 412, 496
AcsI RAATTY 3 cut(s) 15, 545, 587
AfiI CCNNNNNNNGG 3 cut(s) 57, 58, 128
AgsI TTSAA 3 cut(s) 32, 263, 382
AjnI CCWGG 3 cut(s) 56, 121, 151
AleI CACNNNNGTG 2 cut(s) 390, 420
AloI GAACNNNNNNTCC 2 cut(s) 38, 70
AluBI AGCT 2 cut(s) 169, 356
AluI AGCT 2 cut(s) 169, 356
Alw26I GTCTC 1 cut(s) 30
AlwI GGATC 5 cut(s) 48, 126, 133, 139, 177
AlwNI CAGNNNCTG 1 cut(s) 128
AoxI GGCC 8 cut(s) 72, 78, 90, 154, 226, 412, 496, 601
ApeKI GCWGC 1 cut(s) 630
ApoI RAATTY 3 cut(s) 15, 545, 587
Asp700I GAANNNNTTC 1 cut(s) 365
AspS9I GGNCC 2 cut(s) 119, 227
AsuC2I CCSGG 3 cut(s) 136, 187, 575
AsuHPI GGTGA 1 cut(s) 188
AvaII GGWCC 1 cut(s) 119
BalI TGGCCA 1 cut(s) 414
BamHI GGATCC 1 cut(s) 131
BanII GRGCYC 1 cut(s) 192
BauI CACGAG 1 cut(s) 557
BbsI GAAGAC 1 cut(s) 524
BbvCI CCTCAGC 1 cut(s) 506
BccI CCATC 1 cut(s) 488
BceAI ACGGC 1 cut(s) 483
BciT130I CCWGG 3 cut(s) 58, 123, 153
BcnI CCSGG 3 cut(s) 136, 187, 575
BcoDI GTCTC 1 cut(s) 30
BfaI CTAG 2 cut(s) 113, 620
BisI GCNGC 1 cut(s) 631
BlsI GCNGC 1 cut(s) 632
Bme1390I CCNGG 6 cut(s) 58, 123, 136, 153, 187, 575
Bme18I GGWCC 1 cut(s) 119
BmgT120I GGNCC 2 cut(s) 119, 227
BmiI GGNNCC 2 cut(s) 133, 191
BmrFI CCNGG 6 cut(s) 58, 123, 136, 153, 187, 575
BpiI GAAGAC 1 cut(s) 524
BpmI CTGGAG 1 cut(s) 439
Bpu10I CCTNAGC 2 cut(s) 87, 506
BpuEI CTTGAG 1 cut(s) 222
BpuMI CCSGG 3 cut(s) 136, 187, 575
Bsa29I ATCGAT 1 cut(s) 181
BsaBI GATNNNNATC 1 cut(s) 130
BsaJI CCNNGG 5 cut(s) 57, 69, 75, 81, 499
BsaXI ACNNNNNCTCC 2 cut(s) 292, 322
Bsc4I CCNNNNNNNGG 3 cut(s) 57, 58, 128
Bse1I ACTGG 1 cut(s) 422
Bse3DI GCAATG 2 cut(s) 409, 591
Bse8I GATNNNNATC 1 cut(s) 130
BseBI CCWGG 3 cut(s) 58, 123, 153
BseCI ATCGAT 1 cut(s) 181
BseDI CCNNGG 5 cut(s) 57, 69, 75, 81, 499
BseGI GGATG 2 cut(s) 499, 568
BseJI GATNNNNATC 1 cut(s) 130
BseLI CCNNNNNNNGG 3 cut(s) 57, 58, 128
BseMI GCAATG 2 cut(s) 409, 591
BseMII CTCAG 3 cut(s) 348, 456, 497
BseNI ACTGG 1 cut(s) 422
BseRI GAGGAG 1 cut(s) 182
BshFI GGCC 8 cut(s) 74, 80, 92, 156, 228, 414, 498, 603
BshVI ATCGAT 1 cut(s) 181
BsiSI CCGG 4 cut(s) 117, 135, 186, 575
BslFI GGGAC 1 cut(s) 94
BslI CCNNNNNNNGG 3 cut(s) 57, 58, 128
BsmAI GTCTC 1 cut(s) 30
BsmBI CGTCTC 1 cut(s) 30
BsmFI GGGAC 1 cut(s) 94
BsmI GAATGC 1 cut(s) 347
BsnI GGCC 8 cut(s) 74, 80, 92, 156, 228, 414, 498, 603
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 5 cut(s) 53, 125, 131, 178, 182
BspANI GGCC 8 cut(s) 74, 80, 92, 156, 228, 414, 498, 603
BspCNI CTCAG 3 cut(s) 349, 457, 498
BspDI ATCGAT 1 cut(s) 181
BspLI GGNNCC 2 cut(s) 133, 191
BspPI GGATC 5 cut(s) 48, 126, 133, 139, 177
BsrDI GCAATG 2 cut(s) 409, 591
BsrI ACTGG 1 cut(s) 422
BssECI CCNNGG 5 cut(s) 57, 69, 75, 81, 499
BssMI GATC 5 cut(s) 53, 125, 131, 178, 182
BssNAI GTATAC 1 cut(s) 449
BssSI CACGAG 1 cut(s) 557
BssT1I CCWWGG 3 cut(s) 69, 75, 81
Bst1107I GTATAC 1 cut(s) 449
Bst2BI CACGAG 1 cut(s) 557
Bst2UI CCWGG 3 cut(s) 58, 123, 153
Bst6I CTCTTC 3 cut(s) 204, 258, 321
BstDEI CTNAG 4 cut(s) 87, 357, 465, 506
BstDSI CCRYGG 1 cut(s) 499
BstF5I GGATG 2 cut(s) 499, 568
BstKTI GATC 5 cut(s) 56, 128, 134, 181, 185
BstMAI GTCTC 1 cut(s) 30
BstMBI GATC 5 cut(s) 53, 125, 131, 178, 182
BstMWI GCNNNNNNNGC 1 cut(s) 353
BstNI CCWGG 3 cut(s) 58, 123, 153
BstSCI CCNGG 6 cut(s) 56, 121, 134, 151, 185, 573
BstV2I GAAGAC 1 cut(s) 524
BstX2I RGATCY 2 cut(s) 125, 131
BstYI RGATCY 2 cut(s) 125, 131
BstZ17I GTATAC 1 cut(s) 449
Bsu15I ATCGAT 1 cut(s) 181
BsuRI GGCC 8 cut(s) 74, 80, 92, 156, 228, 414, 498, 603
BsuTUI ATCGAT 1 cut(s) 181
BtgI CCRYGG 1 cut(s) 499
BtsCI GGATG 2 cut(s) 499, 568
BtsIMutI CAGTG 1 cut(s) 415
CaiI CAGNNNCTG 1 cut(s) 128
Cfr13I GGNCC 2 cut(s) 119, 227
ClaI ATCGAT 1 cut(s) 181
CviAII CATG 2 cut(s) 43, 429
DdeI CTNAG 4 cut(s) 87, 357, 465, 506
DpnI GATC 5 cut(s) 55, 127, 133, 180, 184
DpnII GATC 5 cut(s) 53, 125, 131, 178, 182
EaeI YGGCCR 2 cut(s) 412, 496
Eam1104I CTCTTC 3 cut(s) 204, 258, 321
EarI CTCTTC 3 cut(s) 204, 258, 321
Eco130I CCWWGG 3 cut(s) 69, 75, 81
Eco24I GRGCYC 1 cut(s) 192
Eco47I GGWCC 1 cut(s) 119
EcoRII CCWGG 3 cut(s) 56, 121, 151
EcoT14I CCWWGG 3 cut(s) 69, 75, 81
EcoT38I GRGCYC 1 cut(s) 192
ErhI CCWWGG 3 cut(s) 69, 75, 81
Esp3I CGTCTC 1 cut(s) 30
FaeI CATG 2 cut(s) 46, 432
FaiI YATR 8 cut(s) 44, 223, 225, 430, 449, 485, 513, 521
FaqI GGGAC 1 cut(s) 94
FatI CATG 2 cut(s) 42, 428
FauNDI CATATG 1 cut(s) 223
FblI GTMKAC 1 cut(s) 448
Fnu4HI GCNGC 1 cut(s) 631
FokI GGATG 2 cut(s) 506, 575
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 1 cut(s) 631
FspBI CTAG 2 cut(s) 113, 620
GluI GCNGC 1 cut(s) 631
GsuI CTGGAG 1 cut(s) 439
HaeIII GGCC 8 cut(s) 74, 80, 92, 156, 228, 414, 498, 603
HapII CCGG 4 cut(s) 117, 135, 186, 575
Hin1II CATG 2 cut(s) 46, 432
HinfI GANTC 4 cut(s) 148, 268, 372, 425
HpaII CCGG 4 cut(s) 117, 135, 186, 575
HphI GGTGA 1 cut(s) 188
Hpy166II GTNNAC 1 cut(s) 449
Hpy188I TCNGA 2 cut(s) 173, 371
Hpy188III TCNNGA 2 cut(s) 29, 129
Hpy8I GTNNAC 1 cut(s) 449
HpyAV CCTTC 2 cut(s) 593, 620
HpyCH4IV ACGT 1 cut(s) 445
HpyCH4V TGCA 3 cut(s) 4, 402, 596
HpyF10VI GCNNNNNNNGC 1 cut(s) 353
HpyF3I CTNAG 4 cut(s) 87, 357, 465, 506
HpySE526I ACGT 1 cut(s) 445
Hsp92II CATG 2 cut(s) 46, 432
Kzo9I GATC 5 cut(s) 53, 125, 131, 178, 182
LmnI GCTCC 1 cut(s) 195
MaeI CTAG 2 cut(s) 113, 620
MaeII ACGT 1 cut(s) 445
MaeIII GTNAC 2 cut(s) 460, 551
MalI GATC 5 cut(s) 55, 127, 133, 180, 184
MboI GATC 5 cut(s) 53, 125, 131, 178, 182
MboII GAAGA 6 cut(s) 157, 191, 275, 338, 343, 524
MflI RGATCY 2 cut(s) 125, 131
MhlI GDGCHC 1 cut(s) 192
MlsI TGGCCA 1 cut(s) 414
MluCI AATT 4 cut(s) 15, 377, 545, 587
MluNI TGGCCA 1 cut(s) 414
MmeI TCCRAC 1 cut(s) 307
Mox20I TGGCCA 1 cut(s) 414
MroXI GAANNNNTTC 1 cut(s) 365
MscI TGGCCA 1 cut(s) 414
MseI TTAA 1 cut(s) 581
MslI CAYNNNNRTG 3 cut(s) 390, 420, 561
Msp20I TGGCCA 1 cut(s) 414
MspA1I CMGCKG 1 cut(s) 356
MspI CCGG 4 cut(s) 117, 135, 186, 575
MspR9I CCNGG 6 cut(s) 58, 123, 136, 153, 187, 575
Mva1269I GAATGC 1 cut(s) 347
MvaI CCWGG 3 cut(s) 58, 123, 153
MwoI GCNNNNNNNGC 1 cut(s) 353
NciI CCSGG 3 cut(s) 136, 187, 575
NdeI CATATG 1 cut(s) 223
NdeII GATC 5 cut(s) 53, 125, 131, 178, 182
NlaIII CATG 2 cut(s) 46, 432
NlaIV GGNNCC 2 cut(s) 133, 191
NmuCI GTSAC 1 cut(s) 551
OliI CACNNNNGTG 2 cut(s) 390, 420
PctI GAATGC 1 cut(s) 347
PdmI GAANNNNTTC 1 cut(s) 365
PfeI GAWTC 4 cut(s) 148, 268, 372, 425
PflMI CCANNNNNTGG 1 cut(s) 128
PkrI GCNGC 1 cut(s) 632
Psp6I CCWGG 3 cut(s) 56, 121, 151
PspGI CCWGG 3 cut(s) 56, 121, 151
PspN4I GGNNCC 2 cut(s) 133, 191
PspPI GGNCC 2 cut(s) 119, 227
PstNI CAGNNNCTG 1 cut(s) 128
PsuI RGATCY 2 cut(s) 125, 131
PvuII CAGCTG 1 cut(s) 356
RseI CAYNNNNRTG 3 cut(s) 390, 420, 561
SaqAI TTAA 1 cut(s) 581
SatI GCNGC 1 cut(s) 631
Sau3AI GATC 5 cut(s) 53, 125, 131, 178, 182
Sau96I GGNCC 2 cut(s) 119, 227
ScrFI CCNGG 6 cut(s) 58, 123, 136, 153, 187, 575
SduI GDGCHC 1 cut(s) 192
SetI ASST 9 cut(s) 171, 178, 358, 367, 441, 448, 471, 512, 621
SinI GGWCC 1 cut(s) 119
SmiMI CAYNNNNRTG 3 cut(s) 390, 420, 561
SmlI CTYRAG 1 cut(s) 237
SmoI CTYRAG 1 cut(s) 237
Sse9I AATT 4 cut(s) 15, 377, 545, 587
SspI AATATT 1 cut(s) 489
SspMI CTAG 2 cut(s) 113, 620
StyD4I CCNGG 6 cut(s) 56, 121, 134, 151, 185, 573
StyI CCWWGG 3 cut(s) 69, 75, 81
TaiI ACGT 1 cut(s) 448
TaqI TCGA 1 cut(s) 181
TasI AATT 4 cut(s) 15, 377, 545, 587
TfiI GAWTC 4 cut(s) 148, 268, 372, 425
Tru1I TTAA 1 cut(s) 581
Tru9I TTAA 1 cut(s) 581
TscAI CASTG 1 cut(s) 422
TseFI GTSAC 1 cut(s) 551
TseI GCWGC 1 cut(s) 630
Tsp45I GTSAC 1 cut(s) 551
TspDTI ATGAA 4 cut(s) 59, 264, 417, 580
TspGWI ACGGA 1 cut(s) 175
TspRI CASTG 1 cut(s) 422
Van91I CCANNNNNTGG 1 cut(s) 128
VpaK11BI GGWCC 1 cut(s) 119
XapI RAATTY 3 cut(s) 15, 545, 587
XmiI GTMKAC 1 cut(s) 448
XmnI GAANNNNTTC 1 cut(s) 365
XspI CTAG 2 cut(s) 113, 620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.