MD08G1234200.v1.1

Tumour-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000520
Physical Location & Seq
Reverse (-)
30094114 .. 30095891
1778 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1234200.v1.1.491

Sequence Viewer

Length: 552 bp
ATGCATCGCCGACCCAAAGCCACATCAAGGGCGAAGGCTAAGTCCAAGTCGCTCGCCGGACAAGGATCCGGGCATGCGAAGAATCCAATTCCAGCCCAACCGACGAAGCTCCGAGAGAAGAAGAAAGAGAGAAAGGCGGCTGTTAAAGACTCTCCGACGACTCACCTCAAGAATGTAAAGGCTGTTGCAGACTCTCTGGCTCAAATCCAACTAGAGGAGAAGGGTACTTGCCCAGCTTTTTCCAAGGAGGACAAAATACCCAAATGCATAGTCGAGCCACGCATAGTTTCCGAATTGCCATCTAGTTGTCCAATTTGCACTAAGTTTGGCCACGCTCCTGAACAATGTCCATGGAGGAGACGTCTCCCCTTCGGTGTAACTCAAGTTGGCAAAGGCTATGAAATATACGGGAGGCGTGTTCATATCCTTTGGTGTTCTTTCTGTGATGAGATTGGTCACCACAGGACCTCAGAATGCCTGAAGCCGAACAAAAAGATCGAAAAAACTAGATTATGGAAAGAGTTCTTCTTCCCTGAAGATAAGGACTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Membralin

Protein Analysis

184

Amino Acids

20.74

Weight (kDa)

9.77

Isoelectric Point (pI)

66.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000520)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g08811 FvH4_2g13461
malus_domestica MD02G1305800.v1.1 MD02G1311300.v1.1 MD02G1311400.v1.1 MD03G1254800.v1.1 MD04G1165700.v1.1 MD05G1031200.v1.1 MD06G1150500.v1.1 MD06G1150600.v1.1 MD06G1150900.v1.1 MD06G1151100.v1.1 MD06G1151200.v1.1 MD07G1018900.v1.1 MD07G1019400.v1.1 MD07G1019500.v1.1 MD07G1023400.v1.1 MD08G1234000.v1.1 MD08G1234200.v1.1 MD13G1233200.v1.1 MD17G1268200.v1.1
pyrus_communis pycom02g25630 pycom02g26130 pycom05g02240 pycom05g02610 pycom06g13890 pycom06g13900 pycom07g01500 pycom07g01510 pycom07g01520 pycom07g01530 pycom07g01540 pycom08g17170 pycom08g17230 pycom08g20290 pycom13g20570
rosa_chinensis RchiOBHm_Chr2g0139011 RchiOBHm_Chr3g0492121
rosa_laevigata RLG00000002413 RLG00000019773 RLG00000019781 RLG00000022823 RLG00000024839 RLG00000027358
rosa_multiflora Rmu_sc0000379.1_g000005 Rmu_sc0002029.1_g000002 Rmu_sc0009440.1_g000007
rosa_roxburghii Rroxscaffold_2G00105770 Rroxscaffold_2G00105790 Rroxscaffold_2G00105890 Rroxscaffold_3G00241430 Rroxscaffold_4G00290390 Rroxscaffold_4G00290420 Rroxscaffold_7G00207210
rosa_rugosa Rorug02G0354100 Rorug02G0354200 Rorug02G0354300 Rorug02G0354400 Rorug02G0354500 Rorug03G0323900 Rorug07G0173300 Rorug07G0173400
rosa_samantha Rh1AG336500 Rh2AG403700 Rh2AG403900 Rh2BG414300 Rh2BG414400 Rh2BG474400 Rh2CG389800 Rh2CG390000 Rh2DG423900 Rh2DG424000 Rh2DG483000 Rh3CG333300 Rh3DG335400 Rh6AG108100 Rh6BG103000 Rh6BG103400 Rh6CG096600 Rh6DG090300 Rh7AG315900 Rh7BG306200 Rh7DG314100 Rh7DG314600
rosa_wichuraiana Rw2G032870 Rw7G026680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 364
AciI CCGC 1 cut(s) 137
AclWI GGATC 2 cut(s) 60, 73
AcoI YGGCCR 1 cut(s) 328
AcuI CTGAAG 1 cut(s) 500
AcyI GRCGYC 1 cut(s) 361
AfaI GTAC 1 cut(s) 226
AfiI CCNNNNNNNGG 2 cut(s) 27, 214
AluBI AGCT 2 cut(s) 109, 236
AluI AGCT 2 cut(s) 109, 236
Alw26I GTCTC 2 cut(s) 352, 368
AlwI GGATC 2 cut(s) 60, 73
AoxI GGCC 1 cut(s) 328
ArsI GACNNNNNNTTYG 4 cut(s) 26, 58, 255, 287
Asp700I GAANNNNTTC 1 cut(s) 521
AspS9I GGNCC 1 cut(s) 465
AsuC2I CCSGG 1 cut(s) 70
AsuHPI GGTGA 2 cut(s) 155, 449
AvaII GGWCC 1 cut(s) 465
BalI TGGCCA 1 cut(s) 330
BamHI GGATCC 1 cut(s) 65
BccI CCATC 1 cut(s) 307
BcnI CCSGG 1 cut(s) 70
BcoDI GTCTC 2 cut(s) 352, 368
BfaI CTAG 3 cut(s) 212, 303, 507
BisI GCNGC 1 cut(s) 138
BlsI GCNGC 1 cut(s) 139
Bme1390I CCNGG 1 cut(s) 70
Bme18I GGWCC 1 cut(s) 465
BmgT120I GGNCC 1 cut(s) 465
BmiI GGNNCC 1 cut(s) 67
BmrFI CCNGG 1 cut(s) 70
BmsI GCATC 1 cut(s) 13
BpuEI CTTGAG 2 cut(s) 152, 366
BpuMI CCSGG 1 cut(s) 70
BsaHI GRCGYC 1 cut(s) 361
BsaJI CCNNGG 2 cut(s) 243, 350
BsaXI ACNNNNNCTCC 2 cut(s) 346, 376
Bsc4I CCNNNNNNNGG 2 cut(s) 27, 214
BseDI CCNNGG 2 cut(s) 243, 350
BseLI CCNNNNNNNGG 2 cut(s) 27, 214
BseMII CTCAG 1 cut(s) 483
BseRI GAGGAG 2 cut(s) 230, 370
BseYI CCCAGC 1 cut(s) 232
BshFI GGCC 1 cut(s) 330
BsiSI CCGG 2 cut(s) 57, 69
BslI CCNNNNNNNGG 2 cut(s) 27, 214
BsmAI GTCTC 2 cut(s) 352, 368
BsmBI CGTCTC 2 cut(s) 352, 368
BsmI GAATGC 1 cut(s) 479
BsnI GGCC 1 cut(s) 330
Bsp143I GATC 2 cut(s) 65, 495
Bsp19I CCATGG 1 cut(s) 350
BspACI CCGC 1 cut(s) 137
BspANI GGCC 1 cut(s) 330
BspCNI CTCAG 1 cut(s) 482
BspLI GGNNCC 1 cut(s) 67
BspPI GGATC 2 cut(s) 60, 73
BssECI CCNNGG 2 cut(s) 243, 350
BssMI GATC 2 cut(s) 65, 495
BssNI GRCGYC 1 cut(s) 361
BssT1I CCWWGG 2 cut(s) 243, 350
BstACI GRCGYC 1 cut(s) 361
BstC8I GCNNGC 2 cut(s) 54, 75
BstDEI CTNAG 4 cut(s) 39, 321, 469, 549
BstDSI CCRYGG 1 cut(s) 350
BstEII GGTNACC 1 cut(s) 455
BstKTI GATC 2 cut(s) 68, 498
BstMAI GTCTC 2 cut(s) 352, 368
BstMBI GATC 2 cut(s) 65, 495
BstNSI RCATGY 1 cut(s) 77
BstPI GGTNACC 1 cut(s) 455
BstSCI CCNGG 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 65
BstYI RGATCY 1 cut(s) 65
BsuRI GGCC 1 cut(s) 330
BtgI CCRYGG 1 cut(s) 350
Cac8I GCNNGC 2 cut(s) 54, 75
Cfr13I GGNCC 1 cut(s) 465
Csp6I GTAC 1 cut(s) 225
CviAII CATG 2 cut(s) 74, 351
CviQI GTAC 1 cut(s) 225
DdeI CTNAG 4 cut(s) 39, 321, 469, 549
DpnI GATC 2 cut(s) 67, 497
DpnII GATC 2 cut(s) 65, 495
EaeI YGGCCR 1 cut(s) 328
Eco130I CCWWGG 2 cut(s) 243, 350
Eco47I GGWCC 1 cut(s) 465
Eco57I CTGAAG 1 cut(s) 500
Eco91I GGTNACC 1 cut(s) 455
EcoO109I RGGNCCY 1 cut(s) 465
EcoO65I GGTNACC 1 cut(s) 455
EcoT14I CCWWGG 2 cut(s) 243, 350
EcoT22I ATGCAT 2 cut(s) 6, 269
ErhI CCWWGG 2 cut(s) 243, 350
Esp3I CGTCTC 2 cut(s) 352, 368
FaeI CATG 2 cut(s) 77, 354
FaiI YATR 8 cut(s) 75, 269, 284, 352, 399, 406, 423, 514
FatI CATG 2 cut(s) 73, 350
Fnu4HI GCNGC 1 cut(s) 138
Fsp4HI GCNGC 1 cut(s) 138
FspBI CTAG 3 cut(s) 212, 303, 507
GluI GCNGC 1 cut(s) 138
GsaI CCCAGC 1 cut(s) 236
HaeIII GGCC 1 cut(s) 330
HapII CCGG 2 cut(s) 57, 69
Hin1I GRCGYC 1 cut(s) 361
Hin1II CATG 2 cut(s) 77, 354
HinfI GANTC 5 cut(s) 82, 149, 160, 191, 545
HpaII CCGG 2 cut(s) 57, 69
HphI GGTGA 2 cut(s) 155, 449
Hpy188I TCNGA 4 cut(s) 113, 156, 292, 472
Hpy188III TCNNGA 2 cut(s) 169, 338
Hpy99I CGWCG 2 cut(s) 106, 160
HpyAV CCTTC 3 cut(s) 28, 214, 379
HpyCH4IV ACGT 1 cut(s) 361
HpyCH4V TGCA 4 cut(s) 4, 188, 267, 318
HpyF3I CTNAG 4 cut(s) 39, 321, 469, 549
HpySE526I ACGT 1 cut(s) 361
Hsp92I GRCGYC 1 cut(s) 361
Hsp92II CATG 2 cut(s) 77, 354
Kzo9I GATC 2 cut(s) 65, 495
LmnI GCTCC 2 cut(s) 114, 340
LpnPI CCDG 9 cut(s) 70, 82, 105, 182, 246, 351, 448, 491, 546
LweI GCATC 1 cut(s) 13
MaeI CTAG 3 cut(s) 212, 303, 507
MaeII ACGT 1 cut(s) 361
MaeIII GTNAC 2 cut(s) 376, 455
MalI GATC 2 cut(s) 67, 497
MboI GATC 2 cut(s) 65, 495
MboII GAAGA 6 cut(s) 91, 130, 133, 517, 520, 548
MflI RGATCY 1 cut(s) 65
MlsI TGGCCA 1 cut(s) 330
MluCI AATT 3 cut(s) 87, 293, 312
MluNI TGGCCA 1 cut(s) 330
MlyI GAGTC 4 cut(s) 143, 154, 185, 539
MmeI TCCRAC 2 cut(s) 179, 232
MnlI CCTC 6 cut(s) 176, 208, 241, 348, 405, 478
Mox20I TGGCCA 1 cut(s) 330
Mph1103I ATGCAT 2 cut(s) 6, 269
MroXI GAANNNNTTC 1 cut(s) 521
MscI TGGCCA 1 cut(s) 330
MseI TTAA 1 cut(s) 144
Msp20I TGGCCA 1 cut(s) 330
MspI CCGG 2 cut(s) 57, 69
MspR9I CCNGG 1 cut(s) 70
Mva1269I GAATGC 1 cut(s) 479
NciI CCSGG 1 cut(s) 70
NcoI CCATGG 1 cut(s) 350
NdeII GATC 2 cut(s) 65, 495
NlaIII CATG 2 cut(s) 77, 354
NlaIV GGNNCC 1 cut(s) 67
NmuCI GTSAC 1 cut(s) 455
NsiI ATGCAT 2 cut(s) 6, 269
NspI RCATGY 1 cut(s) 77
PaeI GCATGC 1 cut(s) 77
PctI GAATGC 1 cut(s) 479
PdmI GAANNNNTTC 1 cut(s) 521
PfeI GAWTC 1 cut(s) 82
PkrI GCNGC 1 cut(s) 139
PleI GAGTC 4 cut(s) 143, 154, 185, 539
PpsI GAGTC 4 cut(s) 143, 154, 185, 539
PpuMI RGGWCCY 1 cut(s) 465
Psp5II RGGWCCY 1 cut(s) 465
PspEI GGTNACC 1 cut(s) 455
PspFI CCCAGC 1 cut(s) 232
PspN4I GGNNCC 1 cut(s) 67
PspPI GGNCC 1 cut(s) 465
PspPPI RGGWCCY 1 cut(s) 465
PsuI RGATCY 1 cut(s) 65
RsaI GTAC 1 cut(s) 226
RsaNI GTAC 1 cut(s) 225
SaqAI TTAA 1 cut(s) 144
SatI GCNGC 1 cut(s) 138
Sau3AI GATC 2 cut(s) 65, 495
Sau96I GGNCC 1 cut(s) 465
SchI GAGTC 4 cut(s) 143, 154, 185, 539
ScrFI CCNGG 1 cut(s) 70
SetI ASST 5 cut(s) 111, 168, 238, 364, 470
SfaNI GCATC 1 cut(s) 13
SinI GGWCC 1 cut(s) 465
SmlI CTYRAG 2 cut(s) 167, 381
SmoI CTYRAG 2 cut(s) 167, 381
SphI GCATGC 1 cut(s) 77
Sse9I AATT 3 cut(s) 87, 293, 312
SsiI CCGC 1 cut(s) 137
SspMI CTAG 3 cut(s) 212, 303, 507
StyD4I CCNGG 1 cut(s) 68
StyI CCWWGG 2 cut(s) 243, 350
TaiI ACGT 1 cut(s) 364
TaqI TCGA 2 cut(s) 273, 498
TasI AATT 3 cut(s) 87, 293, 312
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 1 cut(s) 82
Tru1I TTAA 1 cut(s) 144
Tru9I TTAA 1 cut(s) 144
TseFI GTSAC 1 cut(s) 455
Tsp45I GTSAC 1 cut(s) 455
TspDTI ATGAA 2 cut(s) 410, 414
VpaK11BI GGWCC 1 cut(s) 465
XceI RCATGY 1 cut(s) 77
XmnI GAANNNNTTC 1 cut(s) 521
XspI CTAG 3 cut(s) 212, 303, 507
ZraI GACGTC 1 cut(s) 362
Zsp2I ATGCAT 2 cut(s) 6, 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.