Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0000327

Physical Location & Seq

Genomic Coordinates Reverse (-)

18024298 .. 18025114

Gene Structure 817 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1109300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 771 bp

ATGGAAAATCGAATCCTATTTGTTGTTAATTGTTATTCTGCTGATGAGCTCACGAGGGCAACCAACAACTTCGATCCTTCTTGCCTTATACAAGATTGTTCACCCACTGAAAATAGTCAATTTCCCAGTTCTGTACATGTTTATGGTGGTTACAAAATGTTCAGTGGTTTTCCACACGACTGGTCAATCATTGTTAAGAAGTTTATGGGGACGGGAGGTGAAGCCAAGTCCATGGCCATTCGAGACATTATTATATCAATGCAGATGAGCAACCATAAAAATATTTTGAAACTCTTGGTCTGCAGCTTGGAGATTTCTGTACCAGCTCTTTTTCATGAGTATGCGATGGAAGGAGTTCTCAACGATCAAGGAGACTGCGTATTGCCAAGCAGCTTGCTAATGCACTTACATATCTCCATACTGCCTGCCTTTCCCATTCCAATCATTCATCGGGATTTAAAACCCAGCTGTATTTGCTTGGGCCATGATTATGTTCCCAAACTCACCAACTTCTCACTCTCCATTTCCATTCCTCCTATGCAGTCTCATGTTGAAGATGATCTGAAAGGGACATTAGGGTACCTTGATCCTTCCTACATGAAGTCTGGTTACATTACACAAAAAAGTGATGTTTATAGCTTTGGTGTGCATTTACTTGTATTCTTAACGGGACAAAAAGTCGTAGATAAATATGACACTGGAGAATACCAATCGATTATTGCATACGTGAAACTACATGTTAGTGAAATCGGGGAGATTCAGACAATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050829 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

257

Amino Acids

28.62

Weight (kDa)

5.71

Isoelectric Point (pI)

41.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	49 - 233	9.3e-12	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	75 - 240	8.4e-15	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000327)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G57700 AT3G57710 AT3G57720 AT3G57730 AT3G57750 AT3G57750 AT3G57770
fragaria_vesca	FvH4_2g03430 FvH4_2g03431 FvH4_2g09830
malus_domestica	MD05G1105000.v1.1 MD05G1105300.v1.1 MD10G1109300.v1.1 MD10G1109400.v1.1 MD10G1109700.v1.1 MD10G1109800.v1.1
prunus_persica	Prupe.8G149300_v2.0.a1 Prupe.8G149400_v2.0.a1 Prupe.8G149500_v2.0.a1 Prupe.8G149600_v2.0.a1 Prupe.8G149700_v2.0.a1 Prupe.8G149800_v2.0.a1
pyrus_communis	pycom05g10130 pycom05g10140 pycom05g10190 pycom05g10200 pycom05g10210 pycom05g10220 pycom10g09470 pycom10g09500 pycom10g09510 pycom10g09520
rosa_chinensis	RchiOBHm_Chr6g0248521 RchiOBHm_Chr6g0248531 RchiOBHm_Chr6g0248551 RchiOBHm_Chr6g0248561 RchiOBHm_Chr6g0248591 RchiOBHm_Chr6g0252471 RchiOBHm_Chr6g0266181 RchiOBHm_Chr6g0266231 RchiOBHm_Chr7g0194481 RchiOBHm_Chr7g0194491 RchiOBHm_Chr7g0194561
rosa_laevigata	RLG00000004197 RLG00000014108 RLG00000015141 RLG00000015142 RLG00000015143 RLG00000015145 RLG00000015148 RLG00000015149
rosa_multiflora	Rmu_sc0002393.1_g000003 Rmu_sc0002553.1_g000019 Rmu_sc0002553.1_g000020 Rmu_sc0002553.1_g000027 Rmu_sc0002553.1_g000029 Rmu_sc0002553.1_g000033 Rmu_sc0003605.1_g000049 Rmu_sc0013794.1_g000007
rosa_roxburghii	Rroxscaffold_174G00435350 Rroxscaffold_3G00261310 Rroxscaffold_7G00201830 Rroxscaffold_7G00212700 Rroxscaffold_7G00213190 Rroxscaffold_7G00213210 Rroxscaffold_7G00213220 Rroxscaffold_7G00213240
rosa_rugosa	Rorug05G0531800 Rorug05G0531900 Rorug05G0532100 Rorug05G0532300 Rorug05G0535800 Rorug05G0535900 Rorug06G0024600 Rorug07G0015900 Rorug07G0016300
rosa_samantha	Rh6AG047700 Rh6AG048000 Rh6AG048100 Rh6AG048800 Rh6AG049000 Rh6AG049100 Rh6AG049200 Rh6AG052200 Rh6AG145300 Rh6BG044000 Rh6BG044200 Rh6BG044500 Rh6BG044800 Rh6BG044900 Rh6BG045000 Rh6CG040700 Rh6CG041100 Rh6CG045000 Rh6CG142400 Rh6CG142500 Rh6DG037100 Rh6DG037300 Rh6DG037400 Rh6DG037500 Rh6DG040700 Rh7BG143800
rosa_wichuraiana	Rw6G004140 Rw6G004190 Rw6G004200 Rw6G004210 Rw6G004220 Rw6G004240 Rw6G004250 Rw6G004350 Rw6G004710 Rw6G012550 Rw7G012270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	579
AccB1I	GGYRCC	1 cut(s)	579
AclWI	GGATC	2 cut(s)	68, 581
AcoI	YGGCCR	1 cut(s)	234
AfaI	GTAC	3 cut(s)	135, 321, 581
AflIII	ACRYGT	2 cut(s)	136, 736
AgsI	TTSAA	2 cut(s)	289, 554
AhdI	GACNNNNNGTC	1 cut(s)	677
AluBI	AGCT	6 cut(s)	49, 306, 326, 393, 468, 639
AluI	AGCT	6 cut(s)	49, 306, 326, 393, 468, 639
Alw21I	GWGCWC	1 cut(s)	51
Alw26I	GTCTC	3 cut(s)	237, 366, 549
AlwI	GGATC	2 cut(s)	68, 581
AoxI	GGCC	2 cut(s)	234, 481
ApeKI	GCWGC	2 cut(s)	303, 390
Asp700I	GAANNNNTTC	1 cut(s)	354
Asp718I	GGTACC	1 cut(s)	579
AspS9I	GGNCC	1 cut(s)	481
AsuHPI	GGTGA	3 cut(s)	93, 230, 496
BalI	TGGCCA	1 cut(s)	236
BanI	GGYRCC	1 cut(s)	579
BanII	GRGCYC	1 cut(s)	51
BarI	GAAGNNNNNNTAC	2 cut(s)	593, 625
BauI	CACGAG	1 cut(s)	52
Bbv12I	GWGCWC	1 cut(s)	51
BbvI	GCAGC	2 cut(s)	315, 402
BccI	CCATC	1 cut(s)	340
BcoDI	GTCTC	3 cut(s)	237, 366, 549
BfmI	CTRYAG	1 cut(s)	301
BisI	GCNGC	2 cut(s)	304, 391
BlsI	GCNGC	2 cut(s)	305, 392
BmeRI	GACNNNNNGTC	1 cut(s)	677
BmgT120I	GGNCC	1 cut(s)	481
BmiI	GGNNCC	1 cut(s)	581
BmrI	ACTGGG	1 cut(s)	120
BmuI	ACTGGG	1 cut(s)	120
BpmI	CTGGAG	1 cut(s)	720
Bsa29I	ATCGAT	1 cut(s)	713
BsaAI	YACGTR	1 cut(s)	727
BsaJI	CCNNGG	1 cut(s)	231
BsaXI	ACNNNNNCTCC	2 cut(s)	363, 393
Bse1I	ACTGG	3 cut(s)	126, 185, 703
BseCI	ATCGAT	1 cut(s)	713
BseDI	CCNNGG	1 cut(s)	231
BseNI	ACTGG	3 cut(s)	126, 185, 703
BseXI	GCAGC	2 cut(s)	315, 402
BseYI	CCCAGC	1 cut(s)	464
BshFI	GGCC	2 cut(s)	236, 483
BshNI	GGYRCC	1 cut(s)	579
BshVI	ATCGAT	1 cut(s)	713
BsiHKAI	GWGCWC	1 cut(s)	51
BslFI	GGGAC	3 cut(s)	223, 583, 684
BsmAI	GTCTC	3 cut(s)	237, 366, 549
BsmFI	GGGAC	3 cut(s)	223, 583, 684
BsnI	GGCC	2 cut(s)	236, 483
Bsp1286I	GDGCHC	1 cut(s)	51
Bsp1407I	TGTACA	1 cut(s)	133
Bsp143I	GATC	4 cut(s)	73, 364, 559, 586
Bsp19I	CCATGG	1 cut(s)	231
BspANI	GGCC	2 cut(s)	236, 483
BspDI	ATCGAT	1 cut(s)	713
BspHI	TCATGA	1 cut(s)	334
BspLI	GGNNCC	1 cut(s)	581
BspMAI	CTGCAG	1 cut(s)	305
BspPI	GGATC	2 cut(s)	68, 581
BspT107I	GGYRCC	1 cut(s)	579
BsrGI	TGTACA	1 cut(s)	133
BsrI	ACTGG	3 cut(s)	126, 185, 703
BssECI	CCNNGG	1 cut(s)	231
BssMI	GATC	4 cut(s)	73, 364, 559, 586
BssSI	CACGAG	1 cut(s)	52
BssT1I	CCWWGG	1 cut(s)	231
Bst2BI	CACGAG	1 cut(s)	52
BstAUI	TGTACA	1 cut(s)	133
BstBAI	YACGTR	1 cut(s)	727
BstC8I	GCNNGC	2 cut(s)	395, 426
BstDSI	CCRYGG	1 cut(s)	231
BstKTI	GATC	4 cut(s)	76, 367, 562, 589
BstMAI	GTCTC	3 cut(s)	237, 366, 549
BstMBI	GATC	4 cut(s)	73, 364, 559, 586
BstMWI	GCNNNNNNNGC	1 cut(s)	474
BstNSI	RCATGY	2 cut(s)	140, 740
BstSFI	CTRYAG	1 cut(s)	301
BstV1I	GCAGC	2 cut(s)	315, 402
BstXI	CCANNNNNNTGG	2 cut(s)	180, 232
Bsu15I	ATCGAT	1 cut(s)	713
BsuRI	GGCC	2 cut(s)	236, 483
BsuTUI	ATCGAT	1 cut(s)	713
BtgI	CCRYGG	1 cut(s)	231
BtgZI	GCGATG	1 cut(s)	359
BtsIMutI	CAGTG	3 cut(s)	105, 169, 696
Cac8I	GCNNGC	2 cut(s)	395, 426
CciI	TCATGA	1 cut(s)	334
Cfr13I	GGNCC	1 cut(s)	481
ClaI	ATCGAT	1 cut(s)	713
Csp6I	GTAC	3 cut(s)	134, 320, 580
CviAII	CATG	7 cut(s)	137, 232, 335, 485, 548, 598, 737
CviJI	RGCY	9 cut(s)	49, 224, 236, 306, 326, 393, 468, 483, 639
CviKI_1	RGCY	9 cut(s)	49, 224, 236, 306, 326, 393, 468, 483, 639
CviQI	GTAC	3 cut(s)	134, 320, 580
DpnI	GATC	4 cut(s)	75, 366, 561, 588
DpnII	GATC	4 cut(s)	73, 364, 559, 586
DraI	TTTAAA	1 cut(s)	459
DriI	GACNNNNNGTC	1 cut(s)	677
EaeI	YGGCCR	1 cut(s)	234
Eam1105I	GACNNNNNGTC	1 cut(s)	677
Ecl136II	GAGCTC	1 cut(s)	49
Eco130I	CCWWGG	1 cut(s)	231
Eco24I	GRGCYC	1 cut(s)	51
Eco53kI	GAGCTC	1 cut(s)	49
EcoICRI	GAGCTC	1 cut(s)	49
EcoT14I	CCWWGG	1 cut(s)	231
EcoT38I	GRGCYC	1 cut(s)	51
ErhI	CCWWGG	1 cut(s)	231
FaeI	CATG	7 cut(s)	140, 235, 338, 488, 551, 601, 740
FaqI	GGGAC	3 cut(s)	223, 583, 684
FatI	CATG	7 cut(s)	136, 231, 334, 484, 547, 597, 736
Fnu4HI	GCNGC	2 cut(s)	304, 391
FriOI	GRGCYC	1 cut(s)	51
Fsp4HI	GCNGC	2 cut(s)	304, 391
GluI	GCNGC	2 cut(s)	304, 391
GsaI	CCCAGC	1 cut(s)	468
GsuI	CTGGAG	1 cut(s)	720
HaeIII	GGCC	2 cut(s)	236, 483
Hin1II	CATG	7 cut(s)	140, 235, 338, 488, 551, 601, 740
HinfI	GANTC	2 cut(s)	12, 757
HphI	GGTGA	3 cut(s)	93, 230, 496
Hpy166II	GTNNAC	1 cut(s)	101
Hpy188I	TCNGA	2 cut(s)	564, 762
Hpy188III	TCNNGA	4 cut(s)	52, 242, 335, 452
Hpy8I	GTNNAC	1 cut(s)	101
HpyAV	CCTTC	3 cut(s)	87, 344, 600
HpyCH4IV	ACGT	1 cut(s)	726
HpyCH4V	TGCA	6 cut(s)	262, 303, 403, 541, 649, 722
HpyF10VI	GCNNNNNNNGC	1 cut(s)	474
HpySE526I	ACGT	1 cut(s)	726
Hsp92II	CATG	7 cut(s)	140, 235, 338, 488, 551, 601, 740
KpnI	GGTACC	1 cut(s)	583
Kzo9I	GATC	4 cut(s)	73, 364, 559, 586
LpnPI	CCDG	7 cut(s)	139, 166, 336, 438, 478, 591, 684
Lsp1109I	GCAGC	2 cut(s)	315, 402
MaeII	ACGT	1 cut(s)	726
MaeIII	GTNAC	2 cut(s)	149, 608
MalI	GATC	4 cut(s)	75, 366, 561, 588
MboI	GATC	4 cut(s)	73, 364, 559, 586
MboII	GAAGA	1 cut(s)	566
MhlI	GDGCHC	1 cut(s)	51
MlsI	TGGCCA	1 cut(s)	236
MluCI	AATT	2 cut(s)	28, 119
MluNI	TGGCCA	1 cut(s)	236
MnlI	CCTC	3 cut(s)	48, 209, 543
Mox20I	TGGCCA	1 cut(s)	236
MroXI	GAANNNNTTC	1 cut(s)	354
MscI	TGGCCA	1 cut(s)	236
MseI	TTAA	4 cut(s)	27, 195, 458, 665
MslI	CAYNNNNRTG	4 cut(s)	141, 339, 489, 741
Msp20I	TGGCCA	1 cut(s)	236
MspA1I	CMGCKG	1 cut(s)	468
MwoI	GCNNNNNNNGC	1 cut(s)	474
NcoI	CCATGG	1 cut(s)	231
NdeII	GATC	4 cut(s)	73, 364, 559, 586
NlaIII	CATG	7 cut(s)	140, 235, 338, 488, 551, 601, 740
NlaIV	GGNNCC	1 cut(s)	581
NspI	RCATGY	2 cut(s)	140, 740
PagI	TCATGA	1 cut(s)	334
PciI	ACATGT	2 cut(s)	136, 736
PdmI	GAANNNNTTC	1 cut(s)	354
PfeI	GAWTC	2 cut(s)	12, 757
PkrI	GCNGC	2 cut(s)	305, 392
Ppu21I	YACGTR	1 cut(s)	727
PscI	ACATGT	2 cut(s)	136, 736
Psp124BI	GAGCTC	1 cut(s)	51
PspFI	CCCAGC	1 cut(s)	464
PspN4I	GGNNCC	1 cut(s)	581
PspPI	GGNCC	1 cut(s)	481
PstI	CTGCAG	1 cut(s)	305
PvuII	CAGCTG	1 cut(s)	468
RsaI	GTAC	3 cut(s)	135, 321, 581
RsaNI	GTAC	3 cut(s)	134, 320, 580
RseI	CAYNNNNRTG	4 cut(s)	141, 339, 489, 741
SacI	GAGCTC	1 cut(s)	51
SaqAI	TTAA	4 cut(s)	27, 195, 458, 665
SatI	GCNGC	2 cut(s)	304, 391
Sau3AI	GATC	4 cut(s)	73, 364, 559, 586
Sau96I	GGNCC	1 cut(s)	481
SduI	GDGCHC	1 cut(s)	51
SetI	ASST	9 cut(s)	51, 220, 308, 328, 395, 470, 585, 641, 729
SfcI	CTRYAG	1 cut(s)	301
SmiMI	CAYNNNNRTG	4 cut(s)	141, 339, 489, 741
Sse9I	AATT	2 cut(s)	28, 119
SspI	AATATT	1 cut(s)	283
SstI	GAGCTC	1 cut(s)	51
StyI	CCWWGG	1 cut(s)	231
TaiI	ACGT	1 cut(s)	729
TaqI	TCGA	4 cut(s)	10, 72, 241, 713
TasI	AATT	2 cut(s)	28, 119
TatI	WGTACW	1 cut(s)	133
TfiI	GAWTC	2 cut(s)	12, 757
Tru1I	TTAA	4 cut(s)	27, 195, 458, 665
Tru9I	TTAA	4 cut(s)	27, 195, 458, 665
TscAI	CASTG	3 cut(s)	112, 169, 703
TseI	GCWGC	2 cut(s)	303, 390
TspDTI	ATGAA	3 cut(s)	323, 437, 614
TspRI	CASTG	3 cut(s)	112, 169, 703
XceI	RCATGY	2 cut(s)	140, 740
XmnI	GAANNNNTTC	1 cut(s)	354

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.