in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 14 |
| Acc36I | ACCTGC | 1 cut(s) | 14 |
| AcoI | YGGCCR | 1 cut(s) | 679 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 191 |
| AgsI | TTSAA | 1 cut(s) | 79 |
| AjiI | CACGTC | 1 cut(s) | 109 |
| AluBI | AGCT | 2 cut(s) | 240, 559 |
| AluI | AGCT | 2 cut(s) | 240, 559 |
| Alw21I | GWGCWC | 1 cut(s) | 339 |
| Alw26I | GTCTC | 1 cut(s) | 479 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 29 |
| AoxI | GGCC | 3 cut(s) | 45, 217, 679 |
| ApeKI | GCWGC | 5 cut(s) | 53, 72, 119, 556, 640 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 454 |
| AspLEI | GCGC | 1 cut(s) | 645 |
| Bbv12I | GWGCWC | 1 cut(s) | 339 |
| BbvCI | CCTCAGC | 1 cut(s) | 236 |
| BbvI | GCAGC | 5 cut(s) | 40, 59, 131, 568, 627 |
| BccI | CCATC | 1 cut(s) | 143 |
| BceAI | ACGGC | 3 cut(s) | 32, 97, 477 |
| BciVI | GTATCC | 1 cut(s) | 178 |
| BclI | TGATCA | 1 cut(s) | 523 |
| BcoDI | GTCTC | 1 cut(s) | 479 |
| BfaI | CTAG | 1 cut(s) | 17 |
| BfuAI | ACCTGC | 1 cut(s) | 14 |
| BfuI | GTATCC | 1 cut(s) | 178 |
| BisI | GCNGC | 5 cut(s) | 54, 73, 120, 557, 641 |
| BlsI | GCNGC | 5 cut(s) | 55, 74, 121, 558, 642 |
| BmgBI | CACGTC | 1 cut(s) | 109 |
| BmsI | GCATC | 2 cut(s) | 306, 333 |
| BoxI | GACNNNNGTC | 1 cut(s) | 318 |
| BpmI | CTGGAG | 1 cut(s) | 581 |
| Bpu10I | CCTNAGC | 1 cut(s) | 236 |
| BsaJI | CCNNGG | 2 cut(s) | 48, 606 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 191 |
| Bse1I | ACTGG | 2 cut(s) | 599, 682 |
| BseDI | CCNNGG | 2 cut(s) | 48, 606 |
| BseGI | GGATG | 3 cut(s) | 135, 411, 507 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 191 |
| BseMII | CTCAG | 5 cut(s) | 29, 250, 277, 345, 408 |
| BseNI | ACTGG | 2 cut(s) | 599, 682 |
| BseRI | GAGGAG | 4 cut(s) | 152, 212, 290, 578 |
| BseXI | GCAGC | 5 cut(s) | 40, 59, 131, 568, 627 |
| BsgI | GTGCAG | 2 cut(s) | 193, 330 |
| BshFI | GGCC | 3 cut(s) | 47, 219, 681 |
| BsiHKAI | GWGCWC | 1 cut(s) | 339 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 191 |
| BsmAI | GTCTC | 1 cut(s) | 479 |
| BsnI | GGCC | 3 cut(s) | 47, 219, 681 |
| Bsp1286I | GDGCHC | 1 cut(s) | 339 |
| Bsp143I | GATC | 2 cut(s) | 408, 523 |
| BspANI | GGCC | 3 cut(s) | 47, 219, 681 |
| BspCNI | CTCAG | 5 cut(s) | 30, 249, 276, 346, 409 |
| BspHI | TCATGA | 2 cut(s) | 63, 480 |
| BspMI | ACCTGC | 1 cut(s) | 14 |
| BspQI | GCTCTTC | 1 cut(s) | 652 |
| BsrI | ACTGG | 2 cut(s) | 599, 682 |
| BssECI | CCNNGG | 2 cut(s) | 48, 606 |
| BssMI | GATC | 2 cut(s) | 408, 523 |
| BssT1I | CCWWGG | 1 cut(s) | 606 |
| Bst4CI | ACNGT | 1 cut(s) | 144 |
| Bst6I | CTCTTC | 1 cut(s) | 652 |
| BstC8I | GCNNGC | 1 cut(s) | 231 |
| BstDEI | CTNAG | 6 cut(s) | 38, 236, 263, 354, 417, 488 |
| BstDSI | CCRYGG | 1 cut(s) | 48 |
| BstEII | GGTNACC | 1 cut(s) | 731 |
| BstF5I | GGATG | 3 cut(s) | 135, 411, 507 |
| BstHHI | GCGC | 1 cut(s) | 645 |
| BstKTI | GATC | 2 cut(s) | 411, 526 |
| BstMAI | GTCTC | 1 cut(s) | 479 |
| BstMBI | GATC | 2 cut(s) | 408, 523 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 53, 81, 216, 303 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 318 |
| BstPI | GGTNACC | 1 cut(s) | 731 |
| BstV1I | GCAGC | 5 cut(s) | 40, 59, 131, 568, 627 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 689 |
| BsuI | GTATCC | 1 cut(s) | 178 |
| BsuRI | GGCC | 3 cut(s) | 47, 219, 681 |
| BtgI | CCRYGG | 1 cut(s) | 48 |
| BtrI | CACGTC | 1 cut(s) | 109 |
| BtsCI | GGATG | 3 cut(s) | 135, 411, 507 |
| BtsI | GCAGTG | 1 cut(s) | 313 |
| BtsIMutI | CAGTG | 2 cut(s) | 140, 313 |
| BveI | ACCTGC | 1 cut(s) | 14 |
| Cac8I | GCNNGC | 1 cut(s) | 231 |
| CaiI | CAGNNNCTG | 1 cut(s) | 29 |
| CciI | TCATGA | 2 cut(s) | 63, 480 |
| CfoI | GCGC | 1 cut(s) | 645 |
| CviAII | CATG | 5 cut(s) | 64, 398, 481, 703, 739 |
| DdeI | CTNAG | 6 cut(s) | 38, 236, 263, 354, 417, 488 |
| DpnI | GATC | 2 cut(s) | 410, 525 |
| DpnII | GATC | 2 cut(s) | 408, 523 |
| EaeI | YGGCCR | 1 cut(s) | 679 |
| Eam1104I | CTCTTC | 1 cut(s) | 652 |
| EarI | CTCTTC | 1 cut(s) | 652 |
| Eco130I | CCWWGG | 1 cut(s) | 606 |
| Eco147I | AGGCCT | 1 cut(s) | 219 |
| Eco91I | GGTNACC | 1 cut(s) | 731 |
| EcoO65I | GGTNACC | 1 cut(s) | 731 |
| EcoT14I | CCWWGG | 1 cut(s) | 606 |
| ErhI | CCWWGG | 1 cut(s) | 606 |
| FaeI | CATG | 5 cut(s) | 67, 401, 484, 706, 742 |
| FatI | CATG | 5 cut(s) | 63, 397, 480, 702, 738 |
| FbaI | TGATCA | 1 cut(s) | 523 |
| Fnu4HI | GCNGC | 5 cut(s) | 54, 73, 120, 557, 641 |
| FokI | GGATG | 3 cut(s) | 122, 418, 514 |
| Fsp4HI | GCNGC | 5 cut(s) | 54, 73, 120, 557, 641 |
| FspBI | CTAG | 1 cut(s) | 17 |
| GlaI | GCGC | 1 cut(s) | 644 |
| GluI | GCNGC | 5 cut(s) | 54, 73, 120, 557, 641 |
| GsuI | CTGGAG | 1 cut(s) | 581 |
| HaeIII | GGCC | 3 cut(s) | 47, 219, 681 |
| HhaI | GCGC | 1 cut(s) | 645 |
| Hin1II | CATG | 5 cut(s) | 67, 401, 484, 706, 742 |
| Hin6I | GCGC | 1 cut(s) | 643 |
| HinP1I | GCGC | 1 cut(s) | 643 |
| HinfI | GANTC | 3 cut(s) | 9, 484, 509 |
| Hpy166II | GTNNAC | 1 cut(s) | 387 |
| Hpy188I | TCNGA | 3 cut(s) | 39, 329, 405 |
| Hpy188III | TCNNGA | 6 cut(s) | 64, 97, 245, 481, 499, 521 |
| Hpy8I | GTNNAC | 1 cut(s) | 387 |
| Hpy99I | CGWCG | 1 cut(s) | 324 |
| HpyAV | CCTTC | 6 cut(s) | 37, 67, 182, 209, 326, 465 |
| HpyCH4III | ACNGT | 1 cut(s) | 144 |
| HpyCH4IV | ACGT | 2 cut(s) | 108, 747 |
| HpyCH4V | TGCA | 2 cut(s) | 210, 311 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 53, 81, 216, 303 |
| HpyF3I | CTNAG | 6 cut(s) | 38, 236, 263, 354, 417, 488 |
| HpySE526I | ACGT | 2 cut(s) | 108, 747 |
| Hsp92II | CATG | 5 cut(s) | 67, 401, 484, 706, 742 |
| HspAI | GCGC | 1 cut(s) | 643 |
| Ksp22I | TGATCA | 1 cut(s) | 523 |
| Kzo9I | GATC | 2 cut(s) | 408, 523 |
| LguI | GCTCTTC | 1 cut(s) | 652 |
| Lsp1109I | GCAGC | 5 cut(s) | 40, 59, 131, 568, 627 |
| LweI | GCATC | 2 cut(s) | 306, 333 |
| MaeI | CTAG | 1 cut(s) | 17 |
| MaeII | ACGT | 2 cut(s) | 108, 747 |
| MaeIII | GTNAC | 3 cut(s) | 109, 245, 731 |
| MalI | GATC | 2 cut(s) | 410, 525 |
| MboI | GATC | 2 cut(s) | 408, 523 |
| MboII | GAAGA | 2 cut(s) | 26, 639 |
| MfeI | CAATTG | 1 cut(s) | 709 |
| MhlI | GDGCHC | 1 cut(s) | 339 |
| MluCI | AATT | 2 cut(s) | 515, 709 |
| MmeI | TCCRAC | 3 cut(s) | 383, 427, 700 |
| MroXI | GAANNNNTTC | 1 cut(s) | 454 |
| MspA1I | CMGCKG | 1 cut(s) | 559 |
| MunI | CAATTG | 1 cut(s) | 709 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 53, 81, 216, 303 |
| NdeII | GATC | 2 cut(s) | 408, 523 |
| NlaIII | CATG | 5 cut(s) | 67, 401, 484, 706, 742 |
| NmeAIII | GCCGAG | 1 cut(s) | 657 |
| NmuCI | GTSAC | 2 cut(s) | 109, 245 |
| PagI | TCATGA | 2 cut(s) | 63, 480 |
| PaqCI | CACCTGC | 1 cut(s) | 14 |
| PceI | AGGCCT | 1 cut(s) | 219 |
| PciSI | GCTCTTC | 1 cut(s) | 652 |
| PdmI | GAANNNNTTC | 1 cut(s) | 454 |
| PfeI | GAWTC | 3 cut(s) | 9, 484, 509 |
| PflFI | GACNNNGTC | 1 cut(s) | 107 |
| PkrI | GCNGC | 5 cut(s) | 55, 74, 121, 558, 642 |
| PshAI | GACNNNNGTC | 1 cut(s) | 318 |
| PspEI | GGTNACC | 1 cut(s) | 731 |
| PstNI | CAGNNNCTG | 1 cut(s) | 29 |
| PsyI | GACNNNGTC | 1 cut(s) | 107 |
| PvuII | CAGCTG | 1 cut(s) | 559 |
| SapI | GCTCTTC | 1 cut(s) | 652 |
| SatI | GCNGC | 5 cut(s) | 54, 73, 120, 557, 641 |
| Sau3AI | GATC | 2 cut(s) | 408, 523 |
| SduI | GDGCHC | 1 cut(s) | 339 |
| SfaNI | GCATC | 2 cut(s) | 306, 333 |
| Sse9I | AATT | 2 cut(s) | 515, 709 |
| SseBI | AGGCCT | 1 cut(s) | 219 |
| SspMI | CTAG | 1 cut(s) | 17 |
| StuI | AGGCCT | 1 cut(s) | 219 |
| StyI | CCWWGG | 1 cut(s) | 606 |
| TaaI | ACNGT | 1 cut(s) | 144 |
| TaiI | ACGT | 2 cut(s) | 111, 750 |
| TaqI | TCGA | 3 cut(s) | 98, 175, 393 |
| TasI | AATT | 2 cut(s) | 515, 709 |
| TfiI | GAWTC | 3 cut(s) | 9, 484, 509 |
| TscAI | CASTG | 2 cut(s) | 147, 313 |
| TseFI | GTSAC | 2 cut(s) | 109, 245 |
| TseI | GCWGC | 5 cut(s) | 53, 72, 119, 556, 640 |
| Tsp45I | GTSAC | 2 cut(s) | 109, 245 |
| TspDTI | ATGAA | 8 cut(s) | 49, 82, 444, 497, 518, 536, 639, 684 |
| TspRI | CASTG | 2 cut(s) | 147, 313 |
| Tth111I | GACNNNGTC | 1 cut(s) | 107 |
| XmnI | GAANNNNTTC | 1 cut(s) | 454 |
| XspI | CTAG | 1 cut(s) | 17 |