RLG00000028247

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000347
Physical Location & Seq
Reverse (-)
20537680 .. 20541172
3493 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028247

Sequence Viewer

Length: 984 bp
ATGTGGAGGTGGTTCGACCTCAGGCTTGAAGCTCCCAAAGCGATTTCGCTGAAGGAGGCCGTCGGAGCCATGGCTCAAGTAGCCTTAACCGACATAGAGCTCGACGACAGCCCCCAAAAGGCCAACCTCTGCGTCACTCCGGAGATGGTCGCACTAGTTTCCAAAACCCAGGCTACTGATGCCGTCAGCGTGCTGCTGATGCCGAAACCGTTGATGTCGAGCTTCAACTGCTATGAAAGCCGGAATCTGCAGTTCAATCTGGAAATCCTCCACCGCCAATTGCAGCTGAACAATCGGCATGCACTCATTCTTTATGGTGAGGTAGAAAGTAAGGATATGCTTGGCTATGAAGGTCCTGACAGGTTGATTCACTCCTTTAAAATGCCACTGCTTTCATGGAATCGGAATGTGATGGACCTAAGATTCTTAGCAAATCAATATCCAGTGCGCCTTGGCTTGTCTTCAGCGAGATTTTGTGAGCTTATCACTGATATGGATTGTTTTGGGTGTACAGTTCATGCCACACTGTTCGAAAATGAGGTTCTTTTCCGTGTAGTGAATTACGAGCGTGTCTTCCCAACAGAGCCCTCAAGATGCGAGACTGAGAGTGATCATGGATTTCCGTGTGCGTTGAAGTTTGATATCCAGCACATAAAGGCGCTTCTCAATGCCGCTACTTTGTCAGAGATGATATGGATATGTCGACTTCTCTACATTGATGGCGACTCTCTCTCTCTGTCTGCTCGGCCTAGATCTCGGCGGTGTTCCTCCGACGACGGCTGTGTGAGTGTGGCGTCCTTAATCTCGGTGAAACGACGTCACTCTATGGACCGAAGAGTGGTACAGAGCGACGTCGTTTCACCCATAGACGGCGAAGATCCGATCGGCTTCGCGACCCTGAATTCCGATAGGTACTCATGTTATATTGTCTTCAATTTTGCGGTTTATTTTCTGGGGAGTTCCTTAAACGGGGCCAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

328

Amino Acids

37.01

Weight (kDa)

5.84

Isoelectric Point (pI)

49.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000347)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09240 FvH4_7g13760 FvH4_7g13770 FvH4_7g13770 FvH4_7g13770 FvH4_7g13770 FvH4_7g13770 FvH4_7g13770 FvH4_7g13770 FvH4_7g13770 FvH4_7g13780
prunus_persica Prupe.2G162800_v2.0.a1 Prupe.2G163000_v2.0.a1 Prupe.2G163100_v2.0.a1 Prupe.2G163200_v2.0.a1 Prupe.2G163300_v2.0.a1 Prupe.2G163400_v2.0.a1 Prupe.2G163600_v2.0.a1 Prupe.2G163700_v2.0.a1
pyrus_communis pycom07g13980
rosa_chinensis RchiOBHm_Chr1g0331901 RchiOBHm_Chr1g0353761 RchiOBHm_Chr1g0353841 RchiOBHm_Chr1g0353861 RchiOBHm_Chr1g0353871 RchiOBHm_Chr1g0353881 RchiOBHm_Chr1g0353981 RchiOBHm_Chr2g0131681
rosa_laevigata RLG00000000740 RLG00000028239 RLG00000028240 RLG00000028246 RLG00000028247 RLG00000028248 RLG00000028250 RLG00000028259 RLG00000028419 RLG00000029705
rosa_multiflora Rmu_sc0001763.1_g000004 Rmu_sc0005552.1_g000004 Rmu_sc0005552.1_g000006 Rmu_sc0005947.1_g000015 Rmu_sc0006326.1_g000048 Rmu_sc0006326.1_g000049 Rmu_sc0007658.1_g000010 Rmu_sc0007658.1_g000012 Rmu_sc0021704.1_g000001 Rmu_sc0026369.1_g000001
rosa_roxburghii Rroxscaffold_1G00022360 Rroxscaffold_4G00290550 Rroxscaffold_4G00301410 Rroxscaffold_4G00301420 Rroxscaffold_4G00301500 Rroxscaffold_4G00301510 Rroxscaffold_4G00301520 Rroxscaffold_4G00301540 Rroxscaffold_4G00301670
rosa_rugosa Rorug01G0237800 Rorug01G0238800 Rorug01G0238900 Rorug01G0238900 Rorug01G0238900 Rorug01G0239000 Rorug01G0239100 Rorug01G0239200 Rorug01G0239500 Rorug01G0239500 Rorug01G0239600 Rorug01G0239700
rosa_samantha Rh1AG231800 Rh1AG249800 Rh1AG250400 Rh1AG250500 Rh1AG250700 Rh1AG251000 Rh1AG251100 Rh1BG220100 Rh1BG221000 Rh1BG221100 Rh1BG221300 Rh1BG221500 Rh1BG221600 Rh1BG222000 Rh1BG222600 Rh1BG222700 Rh1CG232400 Rh1CG233200 Rh1CG233400 Rh1CG233500 Rh1CG233700 Rh1CG234200 Rh1CG234300 Rh1CG234400 Rh1CG235000 Rh1CG235100 Rh1CG235200 Rh1DG247100 Rh1DG247900 Rh1DG248000 Rh1DG248200 Rh1DG248500 Rh1DG249400 Rh1DG249500 Rh4BG391700 Rh6AG423500 Rh7BG455700 Rh7CG500500 Rh7DG468700
rosa_wichuraiana Rw1G021870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 820, 855
AccI GTMKAC 1 cut(s) 703
AccII CGCG 1 cut(s) 893
AccIII TCCGGA 1 cut(s) 139
AciI CCGC 4 cut(s) 274, 672, 760, 941
AclWI GGATC 1 cut(s) 872
AcsI RAATTY 1 cut(s) 901
AcuI CTGAAG 2 cut(s) 71, 447
AcyI GRCGYC 3 cut(s) 794, 817, 852
AfaI GTAC 3 cut(s) 511, 843, 914
AfiI CCNNNNNNNGG 4 cut(s) 118, 838, 869, 969
AgsI TTSAA 5 cut(s) 29, 226, 256, 634, 934
AhlI ACTAGT 1 cut(s) 154
AjnI CCWGG 1 cut(s) 168
AluBI AGCT 5 cut(s) 32, 100, 222, 286, 481
AluI AGCT 5 cut(s) 32, 100, 222, 286, 481
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 1 cut(s) 593
AlwI GGATC 1 cut(s) 872
Aor13HI TCCGGA 1 cut(s) 139
AoxI GGCC 4 cut(s) 57, 120, 746, 972
ApeKI GCWGC 2 cut(s) 193, 283
ApoI RAATTY 1 cut(s) 901
AspLEI GCGC 2 cut(s) 450, 661
AspS9I GGNCC 4 cut(s) 353, 415, 829, 972
AsuHPI GGTGA 3 cut(s) 329, 820, 852
AsuII TTCGAA 1 cut(s) 531
AvaII GGWCC 3 cut(s) 353, 415, 829
AxyI CCTNAGG 1 cut(s) 20
BaeI ACNNNNGTAYC 2 cut(s) 904, 937
BanII GRGCYC 2 cut(s) 102, 588
BbsI GAAGAC 3 cut(s) 453, 565, 922
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 2 cut(s) 180, 295
BccI CCATC 3 cut(s) 139, 406, 713
BceAI ACGGC 4 cut(s) 44, 167, 793, 886
BciT130I CCWGG 1 cut(s) 170
BclI TGATCA 1 cut(s) 610
BcoDI GTCTC 1 cut(s) 593
BcuI ACTAGT 1 cut(s) 154
BfaI CTAG 2 cut(s) 155, 750
BfmI CTRYAG 1 cut(s) 248
BfoI RGCGCY 1 cut(s) 662
BglII AGATCT 1 cut(s) 752
BisI GCNGC 3 cut(s) 194, 284, 672
BlsI GCNGC 3 cut(s) 195, 285, 673
Bme1390I CCNGG 1 cut(s) 170
Bme18I GGWCC 3 cut(s) 353, 415, 829
BmgT120I GGNCC 4 cut(s) 353, 415, 829, 972
BmiI GGNNCC 2 cut(s) 67, 973
BmrFI CCNGG 1 cut(s) 170
BmsI GCATC 3 cut(s) 169, 189, 584
BpiI GAAGAC 3 cut(s) 453, 565, 922
Bpu14I TTCGAA 1 cut(s) 531
BpuEI CTTGAG 2 cut(s) 60, 574
BsaHI GRCGYC 3 cut(s) 794, 817, 852
BsaJI CCNNGG 3 cut(s) 69, 168, 451
BsaWI WCCGGW 1 cut(s) 139
Bsc4I CCNNNNNNNGG 4 cut(s) 118, 838, 869, 969
Bse1I ACTGG 1 cut(s) 443
Bse21I CCTNAGG 1 cut(s) 20
BseAI TCCGGA 1 cut(s) 139
BseBI CCWGG 1 cut(s) 170
BseDI CCNNGG 3 cut(s) 69, 168, 451
BseLI CCNNNNNNNGG 4 cut(s) 118, 838, 869, 969
BseMII CTCAG 2 cut(s) 34, 594
BseNI ACTGG 1 cut(s) 443
BseXI GCAGC 2 cut(s) 180, 295
Bsh1236I CGCG 1 cut(s) 893
Bsh1285I CGRYCG 1 cut(s) 885
BshFI GGCC 4 cut(s) 59, 122, 748, 974
BsiEI CGRYCG 1 cut(s) 885
BsiHKAI GWGCWC 1 cut(s) 102
BsiSI CCGG 2 cut(s) 140, 241
BslI CCNNNNNNNGG 4 cut(s) 118, 838, 869, 969
BsmAI GTCTC 1 cut(s) 593
BsnI GGCC 4 cut(s) 59, 122, 748, 974
Bsp119I TTCGAA 1 cut(s) 531
Bsp1286I GDGCHC 2 cut(s) 102, 588
Bsp13I TCCGGA 1 cut(s) 139
Bsp1407I TGTACA 1 cut(s) 509
Bsp143I GATC 4 cut(s) 610, 752, 877, 882
Bsp19I CCATGG 1 cut(s) 69
Bsp68I TCGCGA 1 cut(s) 893
BspACI CCGC 4 cut(s) 274, 672, 760, 941
BspANI GGCC 4 cut(s) 59, 122, 748, 974
BspCNI CTCAG 2 cut(s) 33, 595
BspEI TCCGGA 1 cut(s) 139
BspFNI CGCG 1 cut(s) 893
BspLI GGNNCC 2 cut(s) 67, 973
BspMAI CTGCAG 1 cut(s) 252
BspPI GGATC 1 cut(s) 872
BspT104I TTCGAA 1 cut(s) 531
BsrGI TGTACA 1 cut(s) 509
BsrI ACTGG 1 cut(s) 443
BssECI CCNNGG 3 cut(s) 69, 168, 451
BssMI GATC 4 cut(s) 610, 752, 877, 882
BssNI GRCGYC 3 cut(s) 794, 817, 852
BssT1I CCWWGG 2 cut(s) 69, 451
Bst2UI CCWGG 1 cut(s) 170
Bst4CI ACNGT 3 cut(s) 210, 514, 528
Bst6I CTCTTC 1 cut(s) 829
BstACI GRCGYC 3 cut(s) 794, 817, 852
BstAUI TGTACA 1 cut(s) 509
BstBI TTCGAA 1 cut(s) 531
BstC8I GCNNGC 2 cut(s) 191, 300
BstDEI CTNAG 4 cut(s) 20, 419, 427, 603
BstDSI CCRYGG 1 cut(s) 69
BstFNI CGCG 1 cut(s) 893
BstH2I RGCGCY 1 cut(s) 662
BstHHI GCGC 2 cut(s) 450, 661
BstKTI GATC 4 cut(s) 613, 755, 880, 885
BstMAI GTCTC 1 cut(s) 593
BstMBI GATC 4 cut(s) 610, 752, 877, 882
BstMCI CGRYCG 1 cut(s) 885
BstMWI GCNNNNNNNGC 7 cut(s) 38, 65, 80, 179, 199, 228, 237
BstNI CCWGG 1 cut(s) 170
BstNSI RCATGY 1 cut(s) 302
BstSCI CCNGG 1 cut(s) 168
BstSFI CTRYAG 1 cut(s) 248
BstUI CGCG 1 cut(s) 893
BstV1I GCAGC 2 cut(s) 180, 295
BstV2I GAAGAC 3 cut(s) 453, 565, 922
BstX2I RGATCY 2 cut(s) 752, 877
BstYI RGATCY 2 cut(s) 752, 877
Bsu36I CCTNAGG 1 cut(s) 20
BsuRI GGCC 4 cut(s) 59, 122, 748, 974
BtgI CCRYGG 1 cut(s) 69
BtsI GCAGTG 1 cut(s) 386
BtsIMutI CAGTG 4 cut(s) 386, 450, 486, 524
BtuMI TCGCGA 1 cut(s) 893
Cac8I GCNNGC 2 cut(s) 191, 300
CfoI GCGC 2 cut(s) 450, 661
Cfr13I GGNCC 4 cut(s) 353, 415, 829, 972
CseI GACGC 2 cut(s) 121, 783
Csp6I GTAC 3 cut(s) 510, 842, 913
CviAII CATG 6 cut(s) 70, 299, 396, 518, 614, 918
CviQI GTAC 3 cut(s) 510, 842, 913
DdeI CTNAG 4 cut(s) 20, 419, 427, 603
DpnI GATC 4 cut(s) 612, 754, 879, 884
DpnII GATC 4 cut(s) 610, 752, 877, 882
DraI TTTAAA 1 cut(s) 379
Eam1104I CTCTTC 1 cut(s) 829
EarI CTCTTC 1 cut(s) 829
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 2 cut(s) 69, 451
Eco24I GRGCYC 2 cut(s) 102, 588
Eco32I GATATC 1 cut(s) 643
Eco47I GGWCC 3 cut(s) 353, 415, 829
Eco53kI GAGCTC 1 cut(s) 100
Eco57I CTGAAG 2 cut(s) 71, 447
Eco81I CCTNAGG 1 cut(s) 20
EcoICRI GAGCTC 1 cut(s) 100
EcoO109I RGGNCCY 1 cut(s) 353
EcoRI GAATTC 1 cut(s) 901
EcoRII CCWGG 1 cut(s) 168
EcoRV GATATC 1 cut(s) 643
EcoT14I CCWWGG 2 cut(s) 69, 451
EcoT38I GRGCYC 2 cut(s) 102, 588
ErhI CCWWGG 2 cut(s) 69, 451
FaeI CATG 6 cut(s) 73, 302, 399, 521, 617, 921
FatI CATG 6 cut(s) 69, 298, 395, 517, 613, 917
FbaI TGATCA 1 cut(s) 610
FblI GTMKAC 1 cut(s) 703
Fnu4HI GCNGC 3 cut(s) 194, 284, 672
FriOI GRGCYC 2 cut(s) 102, 588
Fsp4HI GCNGC 3 cut(s) 194, 284, 672
FspBI CTAG 2 cut(s) 155, 750
GlaI GCGC 2 cut(s) 449, 660
GluI GCNGC 3 cut(s) 194, 284, 672
HaeII RGCGCY 1 cut(s) 662
HaeIII GGCC 4 cut(s) 59, 122, 748, 974
HapII CCGG 2 cut(s) 140, 241
HgaI GACGC 2 cut(s) 121, 783
HhaI GCGC 2 cut(s) 450, 661
Hin1I GRCGYC 3 cut(s) 794, 817, 852
Hin1II CATG 6 cut(s) 73, 302, 399, 521, 617, 921
Hin6I GCGC 2 cut(s) 448, 659
HinP1I GCGC 2 cut(s) 448, 659
HincII GTYRAC 1 cut(s) 704
HindII GTYRAC 1 cut(s) 704
HinfI GANTC 5 cut(s) 244, 367, 400, 423, 725
HpaII CCGG 2 cut(s) 140, 241
HphI GGTGA 3 cut(s) 329, 820, 852
Hpy166II GTNNAC 2 cut(s) 510, 704
Hpy188I TCNGA 6 cut(s) 65, 405, 685, 772, 882, 907
Hpy188III TCNNGA 5 cut(s) 140, 260, 356, 591, 892
Hpy8I GTNNAC 2 cut(s) 510, 704
Hpy99I CGWCG 7 cut(s) 65, 107, 776, 779, 819, 854, 857
HpyAV CCTTC 2 cut(s) 46, 344
HpyCH4III ACNGT 3 cut(s) 210, 514, 528
HpyCH4IV ACGT 2 cut(s) 817, 852
HpyCH4V TGCA 3 cut(s) 250, 283, 302
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 65, 80, 179, 199, 228, 237
HpyF3I CTNAG 4 cut(s) 20, 419, 427, 603
HpySE526I ACGT 2 cut(s) 817, 852
Hsp92I GRCGYC 3 cut(s) 794, 817, 852
Hsp92II CATG 6 cut(s) 73, 302, 399, 521, 617, 921
HspAI GCGC 2 cut(s) 448, 659
Kpn2I TCCGGA 1 cut(s) 139
Ksp22I TGATCA 1 cut(s) 610
Kzo9I GATC 4 cut(s) 610, 752, 877, 882
LmnI GCTCC 2 cut(s) 37, 65
Lsp1109I GCAGC 2 cut(s) 180, 295
LweI GCATC 3 cut(s) 169, 189, 584
MaeI CTAG 2 cut(s) 155, 750
MaeII ACGT 2 cut(s) 817, 852
MaeIII GTNAC 2 cut(s) 133, 818
MalI GATC 4 cut(s) 612, 754, 879, 884
MboI GATC 4 cut(s) 610, 752, 877, 882
MboII GAAGA 5 cut(s) 453, 565, 846, 887, 922
MfeI CAATTG 1 cut(s) 278
MflI RGATCY 2 cut(s) 752, 877
MhlI GDGCHC 2 cut(s) 102, 588
MluCI AATT 4 cut(s) 278, 559, 901, 934
MlyI GAGTC 1 cut(s) 719
MmeI TCCRAC 2 cut(s) 43, 795
MnlI CCTC 8 cut(s) 29, 49, 137, 278, 313, 532, 598, 778
MroI TCCGGA 1 cut(s) 139
MseI TTAA 4 cut(s) 86, 378, 800, 965
MslI CAYNNNNRTG 1 cut(s) 491
MspA1I CMGCKG 1 cut(s) 286
MspI CCGG 2 cut(s) 140, 241
MspR9I CCNGG 1 cut(s) 170
MunI CAATTG 1 cut(s) 278
MvaI CCWGG 1 cut(s) 170
MvnI CGCG 1 cut(s) 893
MwoI GCNNNNNNNGC 7 cut(s) 38, 65, 80, 179, 199, 228, 237
NcoI CCATGG 1 cut(s) 69
NdeII GATC 4 cut(s) 610, 752, 877, 882
NlaIII CATG 6 cut(s) 73, 302, 399, 521, 617, 921
NlaIV GGNNCC 2 cut(s) 67, 973
NmeAIII GCCGAG 2 cut(s) 724, 736
NmuCI GTSAC 2 cut(s) 133, 818
NruI TCGCGA 1 cut(s) 893
NspI RCATGY 1 cut(s) 302
NspV TTCGAA 1 cut(s) 531
PaeI GCATGC 1 cut(s) 302
PfeI GAWTC 4 cut(s) 244, 367, 400, 423
PkrI GCNGC 3 cut(s) 195, 285, 673
Ple19I CGATCG 1 cut(s) 885
PleI GAGTC 1 cut(s) 719
PpsI GAGTC 1 cut(s) 719
PpuMI RGGWCCY 1 cut(s) 353
Psp124BI GAGCTC 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 353
Psp6I CCWGG 1 cut(s) 168
PspGI CCWGG 1 cut(s) 168
PspN4I GGNNCC 2 cut(s) 67, 973
PspPI GGNCC 4 cut(s) 353, 415, 829, 972
PspPPI RGGWCCY 1 cut(s) 353
PstI CTGCAG 1 cut(s) 252
PsuI RGATCY 2 cut(s) 752, 877
PvuI CGATCG 1 cut(s) 885
PvuII CAGCTG 1 cut(s) 286
RruI TCGCGA 1 cut(s) 893
RsaI GTAC 3 cut(s) 511, 843, 914
RsaNI GTAC 3 cut(s) 510, 842, 913
RseI CAYNNNNRTG 1 cut(s) 491
SacI GAGCTC 1 cut(s) 102
SalI GTCGAC 1 cut(s) 702
SaqAI TTAA 4 cut(s) 86, 378, 800, 965
SatI GCNGC 3 cut(s) 194, 284, 672
Sau3AI GATC 4 cut(s) 610, 752, 877, 882
Sau96I GGNCC 4 cut(s) 353, 415, 829, 972
SchI GAGTC 1 cut(s) 719
ScrFI CCNGG 1 cut(s) 170
SduI GDGCHC 2 cut(s) 102, 588
SfaNI GCATC 3 cut(s) 169, 189, 584
SfcI CTRYAG 1 cut(s) 248
SfuI TTCGAA 1 cut(s) 531
SinI GGWCC 3 cut(s) 353, 415, 829
SmiMI CAYNNNNRTG 1 cut(s) 491
SmlI CTYRAG 2 cut(s) 75, 589
SmoI CTYRAG 2 cut(s) 75, 589
SpeI ACTAGT 1 cut(s) 154
SphI GCATGC 1 cut(s) 302
Sse9I AATT 4 cut(s) 278, 559, 901, 934
SsiI CCGC 4 cut(s) 274, 672, 760, 941
SspMI CTAG 2 cut(s) 155, 750
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 1 cut(s) 168
StyI CCWWGG 2 cut(s) 69, 451
TaaI ACNGT 3 cut(s) 210, 514, 528
TaiI ACGT 2 cut(s) 820, 855
TaqI TCGA 5 cut(s) 15, 102, 218, 531, 703
TaqII GACCGA 1 cut(s) 846
TasI AATT 4 cut(s) 278, 559, 901, 934
TatI WGTACW 1 cut(s) 509
TauI GCSGC 1 cut(s) 674
TfiI GAWTC 4 cut(s) 244, 367, 400, 423
Tru1I TTAA 4 cut(s) 86, 378, 800, 965
Tru9I TTAA 4 cut(s) 86, 378, 800, 965
TscAI CASTG 4 cut(s) 393, 450, 493, 531
TseFI GTSAC 2 cut(s) 133, 818
TseI GCWGC 2 cut(s) 193, 283
Tsp45I GTSAC 2 cut(s) 133, 818
TspDTI ATGAA 4 cut(s) 249, 363, 384, 506
TspGWI ACGGA 2 cut(s) 539, 612
TspRI CASTG 4 cut(s) 393, 450, 493, 531
VpaK11BI GGWCC 3 cut(s) 353, 415, 829
XapI RAATTY 1 cut(s) 901
XceI RCATGY 1 cut(s) 302
XcmI CCANNNNNNNNNTGG 1 cut(s) 393
XmiI GTMKAC 1 cut(s) 703
XspI CTAG 2 cut(s) 155, 750
ZraI GACGTC 2 cut(s) 818, 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.