Rorug01G0239700

Belongs to the DEAD box helicase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000347
Physical Location & Seq
Reverse (-)
34520030 .. 34527852
7823 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0239700.1

Sequence Viewer

Length: 723 bp
ATGACGGATTCGAATCTGGAGCTACAGGAATCGGGGTGGGAGGAGCTGAGGAGAGAGGCCCGCAAGATCGAGGGAGATCTTGACGTCAAGCTTTCTTCTTATGCTAAACTCGGAGCTCGGTTCACACAAGGAGGAGGTTATGTGGAATCTGGGTCACCACCTGTTGGGTCCAGCAGGTCATGGAAGTCTATGGAAATGGAAATCCAGTCTTTGCTCGAGAAGCTATTGGATATAAATGATTCAATGAGCCGATGTGCTGCTTCTGCTACACCAGCTACTTCTGTTACACAGAAGCTAGCAAGGCATAGAGACATACTTCATGAGTTTACTCAGGAGTTCAGACGAATCAAAGGGAACATAAATTCATTGAGGGAACATGCGGAGCTTCTAACTTCTGTCAGGGATGACATTAGCGAGTACAAGGCATCTGGGAGTATGTCTCCAAGAATGCAAATACTACGGGAGAGAGCTGCTATCCATGGAAGTGTATCTCATATAGATGAGGTGATCAGTCAGGCTCAAACAACAAGGGCAGTCTTGGGCTCTCAAAGGGCTTTGTTTGGAGATGTTCAAGGCAAAGTGAAGAATCTAAGTGACAAGTTCCCTGTTATCCGTGGCGTACTTGGTTCAATTAAAAGGAGGCGATCCAGAGACACAATTATTCTGTCTGCAGTCATTGCAGCTTGTACCTTGTTTCTTATCATCTACTGGCTTTCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

240

Amino Acids

26.77

Weight (kDa)

9.41

Isoelectric Point (pI)

60.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-SNARE_C PF12352 151 - 215 1.5e-16 Snare region anchored in the vesicle membrane C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000347)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g09240 FvH4_7g13760 FvH4_7g13770 FvH4_7g13770 FvH4_7g13770 FvH4_7g13770 FvH4_7g13770 FvH4_7g13770 FvH4_7g13770 FvH4_7g13770 FvH4_7g13780
prunus_persica Prupe.2G162800_v2.0.a1 Prupe.2G163000_v2.0.a1 Prupe.2G163100_v2.0.a1 Prupe.2G163200_v2.0.a1 Prupe.2G163300_v2.0.a1 Prupe.2G163400_v2.0.a1 Prupe.2G163600_v2.0.a1 Prupe.2G163700_v2.0.a1
pyrus_communis pycom07g13980
rosa_chinensis RchiOBHm_Chr1g0331901 RchiOBHm_Chr1g0353761 RchiOBHm_Chr1g0353841 RchiOBHm_Chr1g0353861 RchiOBHm_Chr1g0353871 RchiOBHm_Chr1g0353881 RchiOBHm_Chr1g0353981 RchiOBHm_Chr2g0131681
rosa_laevigata RLG00000000740 RLG00000028239 RLG00000028240 RLG00000028246 RLG00000028247 RLG00000028248 RLG00000028250 RLG00000028259 RLG00000028419 RLG00000029705
rosa_multiflora Rmu_sc0001763.1_g000004 Rmu_sc0005552.1_g000004 Rmu_sc0005552.1_g000006 Rmu_sc0005947.1_g000015 Rmu_sc0006326.1_g000048 Rmu_sc0006326.1_g000049 Rmu_sc0007658.1_g000010 Rmu_sc0007658.1_g000012 Rmu_sc0021704.1_g000001 Rmu_sc0026369.1_g000001
rosa_roxburghii Rroxscaffold_1G00022360 Rroxscaffold_4G00290550 Rroxscaffold_4G00301410 Rroxscaffold_4G00301420 Rroxscaffold_4G00301500 Rroxscaffold_4G00301510 Rroxscaffold_4G00301520 Rroxscaffold_4G00301540 Rroxscaffold_4G00301670
rosa_rugosa Rorug01G0237800 Rorug01G0238800 Rorug01G0238900 Rorug01G0238900 Rorug01G0238900 Rorug01G0239000 Rorug01G0239100 Rorug01G0239200 Rorug01G0239500 Rorug01G0239500 Rorug01G0239600 Rorug01G0239700
rosa_samantha Rh1AG231800 Rh1AG249800 Rh1AG250400 Rh1AG250500 Rh1AG250700 Rh1AG251000 Rh1AG251100 Rh1BG220100 Rh1BG221000 Rh1BG221100 Rh1BG221300 Rh1BG221500 Rh1BG221600 Rh1BG222000 Rh1BG222600 Rh1BG222700 Rh1CG232400 Rh1CG233200 Rh1CG233400 Rh1CG233500 Rh1CG233700 Rh1CG234200 Rh1CG234300 Rh1CG234400 Rh1CG235000 Rh1CG235100 Rh1CG235200 Rh1DG247100 Rh1DG247900 Rh1DG248000 Rh1DG248200 Rh1DG248500 Rh1DG249400 Rh1DG249500 Rh4BG391700 Rh6AG423500 Rh7BG455700 Rh7CG500500 Rh7DG468700
rosa_wichuraiana Rw1G021870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 87
Acc36I ACCTGC 1 cut(s) 165
AccB7I CCANNNNNTGG 1 cut(s) 164
AciI CCGC 2 cut(s) 61, 380
AclWI GGATC 1 cut(s) 639
AcsI RAATTY 1 cut(s) 361
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 3 cut(s) 419, 621, 688
AfiI CCNNNNNNNGG 1 cut(s) 164
AgsI TTSAA 4 cut(s) 243, 572, 630, 717
Alw21I GWGCWC 1 cut(s) 118
Alw26I GTCTC 3 cut(s) 303, 444, 645
AlwI GGATC 1 cut(s) 639
Ama87I CYCGRG 1 cut(s) 215
AoxI GGCC 1 cut(s) 57
ApeKI GCWGC 3 cut(s) 257, 470, 680
ApoI RAATTY 1 cut(s) 361
AspS9I GGNCC 2 cut(s) 58, 168
AsuHPI GGTGA 2 cut(s) 147, 517
AsuII TTCGAA 1 cut(s) 11
AsuNHI GCTAGC 1 cut(s) 295
AvaI CYCGRG 1 cut(s) 215
AvaII GGWCC 1 cut(s) 168
BanII GRGCYC 2 cut(s) 118, 545
Bbv12I GWGCWC 1 cut(s) 118
BbvCI CCTCAGC 1 cut(s) 47
BbvI GCAGC 3 cut(s) 244, 457, 692
BclI TGATCA 1 cut(s) 507
BcoDI GTCTC 3 cut(s) 303, 444, 645
BfaI CTAG 1 cut(s) 296
BfmI CTRYAG 2 cut(s) 23, 669
BfuAI ACCTGC 1 cut(s) 165
BglII AGATCT 1 cut(s) 76
BisI GCNGC 3 cut(s) 258, 471, 681
BlsI GCNGC 3 cut(s) 259, 472, 682
Bme18I GGWCC 1 cut(s) 168
BmeT110I CYCGRG 1 cut(s) 215
BmgT120I GGNCC 2 cut(s) 58, 168
BmiI GGNNCC 1 cut(s) 169
BmsI GCATC 1 cut(s) 434
BmtI GCTAGC 1 cut(s) 299
BplI GAGNNNNNCTC 2 cut(s) 424, 456
BpmI CTGGAG 1 cut(s) 38
Bpu10I CCTNAGC 1 cut(s) 47
Bpu14I TTCGAA 1 cut(s) 11
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 478, 613
BsaXI ACNNNNNCTCC 2 cut(s) 126, 156
Bsc4I CCNNNNNNNGG 1 cut(s) 164
Bse1I ACTGG 2 cut(s) 205, 713
Bse3DI GCAATG 1 cut(s) 675
Bse8I GATNNNNATC 1 cut(s) 12
BseDI CCNNGG 2 cut(s) 478, 613
BseGI GGATG 1 cut(s) 409
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 1 cut(s) 164
BseMI GCAATG 1 cut(s) 675
BseMII CTCAG 2 cut(s) 38, 344
BseNI ACTGG 2 cut(s) 205, 713
BseRI GAGGAG 3 cut(s) 56, 64, 147
BseXI GCAGC 3 cut(s) 244, 457, 692
BshFI GGCC 1 cut(s) 59
BsiHKAI GWGCWC 1 cut(s) 118
BsiHKCI CYCGRG 1 cut(s) 215
BslI CCNNNNNNNGG 1 cut(s) 164
BsmAI GTCTC 3 cut(s) 303, 444, 645
BsmI GAATGC 1 cut(s) 453
BsnI GGCC 1 cut(s) 59
BsoBI CYCGRG 1 cut(s) 215
Bsp119I TTCGAA 1 cut(s) 11
Bsp1286I GDGCHC 2 cut(s) 118, 545
Bsp143I GATC 4 cut(s) 66, 76, 507, 644
Bsp19I CCATGG 1 cut(s) 478
BspACI CCGC 2 cut(s) 61, 380
BspANI GGCC 1 cut(s) 59
BspCNI CTCAG 2 cut(s) 39, 343
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 1 cut(s) 169
BspMAI CTGCAG 1 cut(s) 673
BspMI ACCTGC 1 cut(s) 165
BspOI GCTAGC 1 cut(s) 299
BspPI GGATC 1 cut(s) 639
BspT104I TTCGAA 1 cut(s) 11
BsrDI GCAATG 1 cut(s) 675
BsrI ACTGG 2 cut(s) 205, 713
BssECI CCNNGG 2 cut(s) 478, 613
BssMI GATC 4 cut(s) 66, 76, 507, 644
BssNI GRCGYC 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 478
BstACI GRCGYC 1 cut(s) 84
BstAPI GCANNNNNTGC 1 cut(s) 677
BstBI TTCGAA 1 cut(s) 11
BstC8I GCNNGC 2 cut(s) 61, 297
BstDEI CTNAG 3 cut(s) 47, 330, 590
BstDSI CCRYGG 2 cut(s) 478, 613
BstEII GGTNACC 1 cut(s) 153
BstF5I GGATG 1 cut(s) 409
BstKTI GATC 4 cut(s) 69, 79, 510, 647
BstMAI GTCTC 3 cut(s) 303, 444, 645
BstMBI GATC 4 cut(s) 66, 76, 507, 644
BstMWI GCNNNNNNNGC 5 cut(s) 220, 263, 272, 301, 677
BstNSI RCATGY 1 cut(s) 380
BstPI GGTNACC 1 cut(s) 153
BstSFI CTRYAG 2 cut(s) 23, 669
BstV1I GCAGC 3 cut(s) 244, 457, 692
BstX2I RGATCY 1 cut(s) 76
BstYI RGATCY 1 cut(s) 76
BsuRI GGCC 1 cut(s) 59
BtgI CCRYGG 2 cut(s) 478, 613
BtsCI GGATG 1 cut(s) 409
BveI ACCTGC 1 cut(s) 165
Cac8I GCNNGC 2 cut(s) 61, 297
CciI TCATGA 1 cut(s) 319
Cfr13I GGNCC 2 cut(s) 58, 168
Csp6I GTAC 3 cut(s) 418, 620, 687
CviAII CATG 4 cut(s) 180, 320, 377, 479
CviQI GTAC 3 cut(s) 418, 620, 687
DdeI CTNAG 3 cut(s) 47, 330, 590
DpnI GATC 4 cut(s) 68, 78, 509, 646
DpnII GATC 4 cut(s) 66, 76, 507, 644
Ecl136II GAGCTC 1 cut(s) 116
Eco130I CCWWGG 1 cut(s) 478
Eco24I GRGCYC 2 cut(s) 118, 545
Eco47I GGWCC 1 cut(s) 168
Eco53kI GAGCTC 1 cut(s) 116
Eco88I CYCGRG 1 cut(s) 215
Eco91I GGTNACC 1 cut(s) 153
EcoICRI GAGCTC 1 cut(s) 116
EcoO65I GGTNACC 1 cut(s) 153
EcoT14I CCWWGG 1 cut(s) 478
EcoT38I GRGCYC 2 cut(s) 118, 545
ErhI CCWWGG 1 cut(s) 478
FaeI CATG 4 cut(s) 183, 323, 380, 482
FatI CATG 4 cut(s) 179, 319, 376, 478
FauI CCCGC 1 cut(s) 68
FbaI TGATCA 1 cut(s) 507
Fnu4HI GCNGC 3 cut(s) 258, 471, 681
FokI GGATG 1 cut(s) 416
FriOI GRGCYC 2 cut(s) 118, 545
Fsp4HI GCNGC 3 cut(s) 258, 471, 681
FspBI CTAG 1 cut(s) 296
GluI GCNGC 3 cut(s) 258, 471, 681
GsuI CTGGAG 1 cut(s) 38
HaeIII GGCC 1 cut(s) 59
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 4 cut(s) 183, 323, 380, 482
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 7 cut(s) 8, 13, 29, 146, 239, 345, 586
HphI GGTGA 2 cut(s) 147, 517
Hpy166II GTNNAC 2 cut(s) 123, 327
Hpy188I TCNGA 2 cut(s) 113, 341
Hpy188III TCNNGA 6 cut(s) 17, 80, 217, 320, 332, 648
Hpy8I GTNNAC 2 cut(s) 123, 327
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 3 cut(s) 451, 671, 680
HpyF10VI GCNNNNNNNGC 5 cut(s) 220, 263, 272, 301, 677
HpyF3I CTNAG 3 cut(s) 47, 330, 590
HpySE526I ACGT 1 cut(s) 84
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 4 cut(s) 183, 323, 380, 482
Ksp22I TGATCA 1 cut(s) 507
Kzo9I GATC 4 cut(s) 66, 76, 507, 644
LmnI GCTCC 4 cut(s) 19, 43, 113, 382
Lsp1109I GCAGC 3 cut(s) 244, 457, 692
LweI GCATC 1 cut(s) 434
MaeI CTAG 1 cut(s) 296
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 3 cut(s) 153, 283, 593
MalI GATC 4 cut(s) 68, 78, 509, 646
MboI GATC 4 cut(s) 66, 76, 507, 644
MboII GAAGA 2 cut(s) 87, 595
MflI RGATCY 1 cut(s) 76
MhlI GDGCHC 2 cut(s) 118, 545
MluCI AATT 3 cut(s) 361, 630, 657
MnlI CCTC 9 cut(s) 34, 42, 49, 64, 125, 128, 363, 496, 633
MseI TTAA 1 cut(s) 633
MslI CAYNNNNRTG 2 cut(s) 483, 498
Mva1269I GAATGC 1 cut(s) 453
MwoI GCNNNNNNNGC 5 cut(s) 220, 263, 272, 301, 677
NcoI CCATGG 1 cut(s) 478
NdeII GATC 4 cut(s) 66, 76, 507, 644
NheI GCTAGC 1 cut(s) 295
NlaIII CATG 4 cut(s) 183, 323, 380, 482
NlaIV GGNNCC 1 cut(s) 169
NmuCI GTSAC 2 cut(s) 153, 593
NspI RCATGY 1 cut(s) 380
NspV TTCGAA 1 cut(s) 11
PaeR7I CTCGAG 1 cut(s) 215
PagI TCATGA 1 cut(s) 319
PctI GAATGC 1 cut(s) 453
PfeI GAWTC 7 cut(s) 8, 13, 29, 146, 239, 345, 586
PflMI CCANNNNNTGG 1 cut(s) 164
PkrI GCNGC 3 cut(s) 259, 472, 682
Psp124BI GAGCTC 1 cut(s) 118
PspEI GGTNACC 1 cut(s) 153
PspN4I GGNNCC 1 cut(s) 169
PspPI GGNCC 2 cut(s) 58, 168
PstI CTGCAG 1 cut(s) 673
PsuI RGATCY 1 cut(s) 76
RsaI GTAC 3 cut(s) 419, 621, 688
RsaNI GTAC 3 cut(s) 418, 620, 687
RseI CAYNNNNRTG 2 cut(s) 483, 498
SacI GAGCTC 1 cut(s) 118
SaqAI TTAA 1 cut(s) 633
SatI GCNGC 3 cut(s) 258, 471, 681
Sau3AI GATC 4 cut(s) 66, 76, 507, 644
Sau96I GGNCC 2 cut(s) 58, 168
SduI GDGCHC 2 cut(s) 118, 545
SfaNI GCATC 1 cut(s) 434
SfcI CTRYAG 2 cut(s) 23, 669
Sfr274I CTCGAG 1 cut(s) 215
SfuI TTCGAA 1 cut(s) 11
SinI GGWCC 1 cut(s) 168
SlaI CTCGAG 1 cut(s) 215
SmiMI CAYNNNNRTG 2 cut(s) 483, 498
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 3 cut(s) 361, 630, 657
SsiI CCGC 2 cut(s) 61, 380
SspMI CTAG 1 cut(s) 296
SstI GAGCTC 1 cut(s) 118
StyI CCWWGG 1 cut(s) 478
TaiI ACGT 1 cut(s) 87
TaqI TCGA 3 cut(s) 11, 69, 216
TasI AATT 3 cut(s) 361, 630, 657
TatI WGTACW 1 cut(s) 417
TfiI GAWTC 7 cut(s) 8, 13, 29, 146, 239, 345, 586
Tru1I TTAA 1 cut(s) 633
Tru9I TTAA 1 cut(s) 633
TseFI GTSAC 2 cut(s) 153, 593
TseI GCWGC 3 cut(s) 257, 470, 680
Tsp45I GTSAC 2 cut(s) 153, 593
TspDTI ATGAA 2 cut(s) 308, 354
TspGWI ACGGA 2 cut(s) 20, 602
Van91I CCANNNNNTGG 1 cut(s) 164
VpaK11BI GGWCC 1 cut(s) 168
XapI RAATTY 1 cut(s) 361
XceI RCATGY 1 cut(s) 380
XhoI CTCGAG 1 cut(s) 215
XspI CTAG 1 cut(s) 296
ZraI GACGTC 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.