RLG00000008547

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000383
Physical Location & Seq
Reverse (-)
31844275 .. 31844882
608 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008547

Sequence Viewer

Length: 531 bp
ATGCATCGATTTCATTATAATAGACTGACTTTCAATTGCCTGAGGCACACAACCGGCGGGGGTCTCCGACGCAACGTTTCCAGCAGCAGAAGCAGCGGCGTTCTTGTGTTTGATCTCGAACATGCGCTTGCAGACCTCAAGGATGTAGATCTCACGGATCTTCTCCCTGGCGGCGAAGAAGGCCTCTCGCTCGATGAAGGGAGCCAGGGCTTTGGCCTCGTCGGTGGAGAGGGTGCCGTAGTACCTCCAGGGAGAGAAGGGACTGCTGAGGTTCTTGATAATAATGCCAACGTCCGGGAACAACAGGGCGCCCTTTTCAGCAGCAGAAGCGCCGGTGTTCTTAGCCTTGAGGACCTCCAGCACGCGCTCGCTGATCTCGTCGGAGTAGATGGCTCAGATAACCGTTCTGGAGGCGGCGAAGGCGTCTTCCTCGAAGAGGCGAGCCTTGACTTCGGCCTCGTCGGAAGTGGTGAGGAAATTGAACAGAAGGTCCAGGGCGGTGTTGCGGAGGCCCATGGTCTGCAAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

177

Amino Acids

18.38

Weight (kDa)

4.54

Isoelectric Point (pI)

55.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07180 AT5G07180 AT5G62230 AT5G62230
fragaria_vesca FvH4_7g28290 FvH4_7g28290 FvH4_7g28290 FvH4_7g28290
malus_domestica MD02G1216900.v1.1 MD07G1259500.v1.1 MD17G1114200.v1.1 MD17G1114300.v1.1
prunus_persica Prupe.2G283600_v2.0.a1
pyrus_communis pycom05g04060 pycom07g23280 pycom15g04040
rosa_chinensis RchiOBHm_Chr1g0375261 RchiOBHm_Chr2g0167851 RchiOBHm_Chr4g0390421 RchiOBHm_Chr4g0390431 RchiOBHm_Chr4g0442901 RchiOBHm_Chr4g0442981 RchiOBHm_Chr7g0228991 RchiOBHm_Chr7g0239711
rosa_laevigata RLG00000005460 RLG00000005968 RLG00000005971 RLG00000005973 RLG00000005978 RLG00000008547 RLG00000010012 RLG00000010245 RLG00000026673 RLG00000030046 RLG00000031637
rosa_multiflora Rmu_co8166860.1_g000001 Rmu_co8410161.1_g000001 Rmu_sc0000554.1_g000034 Rmu_sc0001782.1_g000050 Rmu_sc0002096.1_g000036 Rmu_sc0003006.1_g000022 Rmu_sc0003701.1_g000003 Rmu_sc0005877.1_g000004 Rmu_sc0006329.1_g000003 Rmu_sc0006695.1_g000032 Rmu_sc0008108.1_g000003 Rmu_sc0009414.1_g000003 Rmu_sc0010356.1_g000001 Rmu_sc0014119.1_g000002 Rmu_sc0014119.1_g000003 Rmu_sc0014161.1_g000004 Rmu_sc0023009.1_g000001 Rmu_sc0036835.1_g000001 Rmu_ssc0000204.1_g000009
rosa_roxburghii Rroxscaffold_1G00024810 Rroxscaffold_2G00110800 Rroxscaffold_2G00122430 Rroxscaffold_3G00261280 Rroxscaffold_4G00282890 Rroxscaffold_4G00330910 Rroxscaffold_5G00370350 Rroxscaffold_7G00161820
rosa_rugosa Rorug01G0389600 Rorug01G0389700 Rorug01G0389800 Rorug01G0389900 Rorug01G0389900 Rorug01G0390000 Rorug04G0343700 Rorug07G0155300
rosa_samantha Rh1AG400300 Rh1CG377300 Rh1DG394700 Rh2AG367100 Rh2BG612000 Rh2CG350500 Rh2DG389600 Rh3CG058100 Rh4AG396700 Rh4AG397100 Rh4AG397500 Rh4AG397600 Rh4AG397700 Rh4BG003000 Rh4BG127700 Rh4BG370500 Rh4BG393400 Rh4BG409200 Rh4CG035400 Rh4CG166700 Rh4CG408200 Rh4CG424700 Rh4DG028000 Rh4DG028100 Rh4DG403300 Rh4DG403800 Rh6DG080900 Rh7DG202700
rosa_wichuraiana Rw1G035540 Rw4G032760 Rw4G034230 Rw4G034240 Rw4G034260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 18
AccB1I GGYRCC 2 cut(s) 233, 308
AccII CGCG 1 cut(s) 365
AciI CCGC 6 cut(s) 57, 96, 171, 414, 498, 506
AclI AACGTT 1 cut(s) 75
AclWI GGATC 1 cut(s) 165
AcyI GRCGYC 2 cut(s) 309, 423
AfaI GTAC 1 cut(s) 243
AfiI CCNNNNNNNGG 2 cut(s) 294, 436
AgsI TTSAA 2 cut(s) 34, 482
AjnI CCWGG 4 cut(s) 166, 204, 247, 492
AloI GAACNNNNNNTCC 2 cut(s) 474, 506
Alw26I GTCTC 1 cut(s) 68
AlwI GGATC 1 cut(s) 165
AoxI GGCC 4 cut(s) 181, 214, 454, 510
ApeKI GCWGC 3 cut(s) 84, 93, 321
AspLEI GCGC 4 cut(s) 127, 311, 332, 367
AspS9I GGNCC 3 cut(s) 352, 490, 511
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 1 cut(s) 482
AvaII GGWCC 2 cut(s) 352, 490
AxyI CCTNAGG 1 cut(s) 41
BanI GGYRCC 2 cut(s) 233, 308
BbsI GAAGAC 1 cut(s) 418
BbvCI CCTCAGC 1 cut(s) 267
BbvI GCAGC 3 cut(s) 96, 105, 333
BccI CCATC 1 cut(s) 383
BceAI ACGGC 1 cut(s) 221
BciT130I CCWGG 4 cut(s) 168, 206, 249, 494
BcnI CCSGG 1 cut(s) 296
BcoDI GTCTC 1 cut(s) 68
BfoI RGCGCY 2 cut(s) 312, 333
BglII AGATCT 1 cut(s) 148
BisI GCNGC 6 cut(s) 85, 94, 97, 172, 322, 415
BlsI GCNGC 6 cut(s) 86, 95, 98, 173, 323, 416
Bme1390I CCNGG 5 cut(s) 168, 206, 249, 296, 494
Bme18I GGWCC 2 cut(s) 352, 490
BmgT120I GGNCC 3 cut(s) 352, 490, 511
BmiI GGNNCC 3 cut(s) 203, 235, 310
BmrFI CCNGG 5 cut(s) 168, 206, 249, 296, 494
BmsI GCATC 1 cut(s) 13
BpiI GAAGAC 1 cut(s) 418
BpmI CTGGAG 3 cut(s) 231, 341, 429
Bpu10I CCTNAGC 1 cut(s) 267
BpuEI CTTGAG 2 cut(s) 122, 368
BpuMI CCSGG 1 cut(s) 296
Bsa29I ATCGAT 1 cut(s) 7
BsaBI GATNNNNATC 1 cut(s) 147
BsaHI GRCGYC 2 cut(s) 309, 423
BsaI GGTCTC 1 cut(s) 68
BsaJI CCNNGG 5 cut(s) 166, 205, 248, 493, 514
Bsc4I CCNNNNNNNGG 2 cut(s) 294, 436
Bse118I RCCGGY 2 cut(s) 53, 332
Bse21I CCTNAGG 1 cut(s) 41
Bse8I GATNNNNATC 1 cut(s) 147
BseBI CCWGG 4 cut(s) 168, 206, 249, 494
BseCI ATCGAT 1 cut(s) 7
BseDI CCNNGG 5 cut(s) 166, 205, 248, 493, 514
BseGI GGATG 1 cut(s) 148
BseJI GATNNNNATC 1 cut(s) 147
BseLI CCNNNNNNNGG 2 cut(s) 294, 436
BseMII CTCAG 3 cut(s) 32, 258, 408
BseXI GCAGC 3 cut(s) 96, 105, 333
Bsh1236I CGCG 1 cut(s) 365
BshFI GGCC 4 cut(s) 183, 216, 456, 512
BshNI GGYRCC 2 cut(s) 233, 308
BshVI ATCGAT 1 cut(s) 7
BsiSI CCGG 3 cut(s) 54, 295, 333
BslFI GGGAC 1 cut(s) 274
BslI CCNNNNNNNGG 2 cut(s) 294, 436
BsmAI GTCTC 1 cut(s) 68
BsmFI GGGAC 1 cut(s) 274
BsnI GGCC 4 cut(s) 183, 216, 456, 512
Bso31I GGTCTC 1 cut(s) 68
Bsp143I GATC 4 cut(s) 112, 148, 157, 373
Bsp19I CCATGG 1 cut(s) 514
BspACI CCGC 6 cut(s) 57, 96, 171, 414, 498, 506
BspANI GGCC 4 cut(s) 183, 216, 456, 512
BspCNI CTCAG 3 cut(s) 33, 259, 407
BspDI ATCGAT 1 cut(s) 7
BspFNI CGCG 1 cut(s) 365
BspLI GGNNCC 3 cut(s) 203, 235, 310
BspPI GGATC 1 cut(s) 165
BspT107I GGYRCC 2 cut(s) 233, 308
BspTNI GGTCTC 1 cut(s) 68
BsrFI RCCGGY 2 cut(s) 53, 332
BssAI RCCGGY 2 cut(s) 53, 332
BssECI CCNNGG 5 cut(s) 166, 205, 248, 493, 514
BssMI GATC 4 cut(s) 112, 148, 157, 373
BssNI GRCGYC 2 cut(s) 309, 423
BssT1I CCWWGG 1 cut(s) 514
Bst2UI CCWGG 4 cut(s) 168, 206, 249, 494
Bst4CI ACNGT 1 cut(s) 404
Bst6I CTCTTC 1 cut(s) 429
BstACI GRCGYC 2 cut(s) 309, 423
BstC8I GCNNGC 4 cut(s) 129, 363, 369, 442
BstDEI CTNAG 4 cut(s) 41, 267, 341, 394
BstDSI CCRYGG 1 cut(s) 514
BstENI CCTNNNNNAGG 1 cut(s) 434
BstF5I GGATG 1 cut(s) 148
BstFNI CGCG 1 cut(s) 365
BstH2I RGCGCY 2 cut(s) 312, 333
BstHHI GCGC 4 cut(s) 127, 311, 332, 367
BstKTI GATC 4 cut(s) 115, 151, 160, 376
BstMAI GTCTC 1 cut(s) 68
BstMBI GATC 4 cut(s) 112, 148, 157, 373
BstMWI GCNNNNNNNGC 5 cut(s) 90, 93, 180, 327, 420
BstNI CCWGG 4 cut(s) 168, 206, 249, 494
BstNSI RCATGY 1 cut(s) 125
BstSCI CCNGG 5 cut(s) 166, 204, 247, 294, 492
BstUI CGCG 1 cut(s) 365
BstV1I GCAGC 3 cut(s) 96, 105, 333
BstV2I GAAGAC 1 cut(s) 418
BstX2I RGATCY 2 cut(s) 148, 157
BstXI CCANNNNNNTGG 1 cut(s) 212
BstYI RGATCY 2 cut(s) 148, 157
Bsu15I ATCGAT 1 cut(s) 7
Bsu36I CCTNAGG 1 cut(s) 41
BsuRI GGCC 4 cut(s) 183, 216, 456, 512
BsuTUI ATCGAT 1 cut(s) 7
BtgI CCRYGG 1 cut(s) 514
BtsCI GGATG 1 cut(s) 148
Cac8I GCNNGC 4 cut(s) 129, 363, 369, 442
CfoI GCGC 4 cut(s) 127, 311, 332, 367
Cfr10I RCCGGY 2 cut(s) 53, 332
Cfr13I GGNCC 3 cut(s) 352, 490, 511
ClaI ATCGAT 1 cut(s) 7
CseI GACGC 2 cut(s) 78, 412
Csp6I GTAC 1 cut(s) 242
CviAII CATG 2 cut(s) 122, 515
CviJI RGCY 9 cut(s) 183, 204, 210, 216, 345, 393, 444, 456, 512
CviKI_1 RGCY 9 cut(s) 183, 204, 210, 216, 345, 393, 444, 456, 512
CviQI GTAC 1 cut(s) 242
DdeI CTNAG 4 cut(s) 41, 267, 341, 394
DinI GGCGCC 1 cut(s) 310
DpnI GATC 4 cut(s) 114, 150, 159, 375
DpnII GATC 4 cut(s) 112, 148, 157, 373
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
Eco130I CCWWGG 1 cut(s) 514
Eco147I AGGCCT 1 cut(s) 183
Eco31I GGTCTC 1 cut(s) 68
Eco47I GGWCC 2 cut(s) 352, 490
Eco81I CCTNAGG 1 cut(s) 41
EcoNI CCTNNNNNAGG 1 cut(s) 434
EcoO109I RGGNCCY 1 cut(s) 352
EcoRII CCWGG 4 cut(s) 166, 204, 247, 492
EcoT14I CCWWGG 1 cut(s) 514
EcoT22I ATGCAT 1 cut(s) 6
EgeI GGCGCC 1 cut(s) 310
EheI GGCGCC 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 514
FaeI CATG 2 cut(s) 125, 518
FaiI YATR 3 cut(s) 18, 123, 516
FaqI GGGAC 1 cut(s) 274
FatI CATG 2 cut(s) 121, 514
FauI CCCGC 1 cut(s) 50
Fnu4HI GCNGC 6 cut(s) 85, 94, 97, 172, 322, 415
FokI GGATG 1 cut(s) 155
Fsp4HI GCNGC 6 cut(s) 85, 94, 97, 172, 322, 415
GlaI GCGC 4 cut(s) 126, 310, 331, 366
GluI GCNGC 6 cut(s) 85, 94, 97, 172, 322, 415
GsuI CTGGAG 3 cut(s) 231, 341, 429
HaeII RGCGCY 2 cut(s) 312, 333
HaeIII GGCC 4 cut(s) 183, 216, 456, 512
HapII CCGG 3 cut(s) 54, 295, 333
HgaI GACGC 2 cut(s) 78, 412
HhaI GCGC 4 cut(s) 127, 311, 332, 367
Hin1I GRCGYC 2 cut(s) 309, 423
Hin1II CATG 2 cut(s) 125, 518
Hin6I GCGC 4 cut(s) 125, 309, 330, 365
HinP1I GCGC 4 cut(s) 125, 309, 330, 365
HpaII CCGG 3 cut(s) 54, 295, 333
HphI GGTGA 1 cut(s) 482
Hpy188I TCNGA 4 cut(s) 68, 383, 397, 464
Hpy188III TCNNGA 3 cut(s) 116, 275, 408
Hpy99I CGWCG 4 cut(s) 72, 224, 383, 464
HpyAV CCTTC 5 cut(s) 173, 191, 251, 413, 481
HpyCH4III ACNGT 1 cut(s) 404
HpyCH4IV ACGT 2 cut(s) 75, 291
HpyCH4V TGCA 3 cut(s) 4, 131, 523
HpyF10VI GCNNNNNNNGC 5 cut(s) 90, 93, 180, 327, 420
HpyF3I CTNAG 4 cut(s) 41, 267, 341, 394
HpySE526I ACGT 2 cut(s) 75, 291
Hsp92I GRCGYC 2 cut(s) 309, 423
Hsp92II CATG 2 cut(s) 125, 518
HspAI GCGC 4 cut(s) 125, 309, 330, 365
KasI GGCGCC 1 cut(s) 308
Kzo9I GATC 4 cut(s) 112, 148, 157, 373
LmnI GCTCC 1 cut(s) 201
Lsp1109I GCAGC 3 cut(s) 96, 105, 333
LweI GCATC 1 cut(s) 13
MaeII ACGT 2 cut(s) 75, 291
MalI GATC 4 cut(s) 114, 150, 159, 375
MboI GATC 4 cut(s) 112, 148, 157, 373
MboII GAAGA 4 cut(s) 152, 188, 418, 446
MfeI CAATTG 1 cut(s) 34
MflI RGATCY 2 cut(s) 148, 157
MluCI AATT 2 cut(s) 34, 477
Mly113I GGCGCC 1 cut(s) 309
MmeI TCCRAC 3 cut(s) 91, 361, 442
Mph1103I ATGCAT 1 cut(s) 6
MspA1I CMGCKG 1 cut(s) 96
MspI CCGG 3 cut(s) 54, 295, 333
MspR9I CCNGG 5 cut(s) 168, 206, 249, 296, 494
MunI CAATTG 1 cut(s) 34
MvaI CCWGG 4 cut(s) 168, 206, 249, 494
MvnI CGCG 1 cut(s) 365
MwoI GCNNNNNNNGC 5 cut(s) 90, 93, 180, 327, 420
NarI GGCGCC 1 cut(s) 309
NciI CCSGG 1 cut(s) 296
NcoI CCATGG 1 cut(s) 514
NdeII GATC 4 cut(s) 112, 148, 157, 373
NlaIII CATG 2 cut(s) 125, 518
NlaIV GGNNCC 3 cut(s) 203, 235, 310
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 125
PceI AGGCCT 1 cut(s) 183
PcsI WCGNNNNNNNCGW 1 cut(s) 375
PfoI TCCNGGA 1 cut(s) 294
PkrI GCNGC 6 cut(s) 86, 95, 98, 173, 323, 416
PluTI GGCGCC 1 cut(s) 312
PpuMI RGGWCCY 1 cut(s) 352
PsiI TTATAA 1 cut(s) 18
Psp1406I AACGTT 1 cut(s) 75
Psp5II RGGWCCY 1 cut(s) 352
Psp6I CCWGG 4 cut(s) 166, 204, 247, 492
PspGI CCWGG 4 cut(s) 166, 204, 247, 492
PspN4I GGNNCC 3 cut(s) 203, 235, 310
PspPI GGNCC 3 cut(s) 352, 490, 511
PspPPI RGGWCCY 1 cut(s) 352
PsuI RGATCY 2 cut(s) 148, 157
RsaI GTAC 1 cut(s) 243
RsaNI GTAC 1 cut(s) 242
SatI GCNGC 6 cut(s) 85, 94, 97, 172, 322, 415
Sau3AI GATC 4 cut(s) 112, 148, 157, 373
Sau96I GGNCC 3 cut(s) 352, 490, 511
ScrFI CCNGG 5 cut(s) 168, 206, 249, 296, 494
SetI ASST 7 cut(s) 78, 138, 247, 273, 294, 357, 492
SfaNI GCATC 1 cut(s) 13
SfoI GGCGCC 1 cut(s) 310
SgrAI CRCCGGYG 1 cut(s) 332
SinI GGWCC 2 cut(s) 352, 490
SmlI CTYRAG 2 cut(s) 137, 347
SmoI CTYRAG 2 cut(s) 137, 347
Sse9I AATT 2 cut(s) 34, 477
SseBI AGGCCT 1 cut(s) 183
SsiI CCGC 6 cut(s) 57, 96, 171, 414, 498, 506
SspDI GGCGCC 1 cut(s) 308
StuI AGGCCT 1 cut(s) 183
StyD4I CCNGG 5 cut(s) 166, 204, 247, 294, 492
StyI CCWWGG 1 cut(s) 514
TaaI ACNGT 1 cut(s) 404
TaiI ACGT 2 cut(s) 78, 294
TaqI TCGA 4 cut(s) 7, 117, 192, 432
TasI AATT 2 cut(s) 34, 477
TauI GCSGC 3 cut(s) 99, 174, 417
TseI GCWGC 3 cut(s) 84, 93, 321
TspDTI ATGAA 1 cut(s) 210
TspGWI ACGGA 1 cut(s) 170
VpaK11BI GGWCC 2 cut(s) 352, 490
XagI CCTNNNNNAGG 1 cut(s) 434
XceI RCATGY 1 cut(s) 125
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.