Rh1CG377300
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0000383
Physical Location & Seq
Forward (+)
64577761 .. 64584876
7116 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG377300.1

Sequence Viewer

Length: 2958 bp
ATGGAGGAGGCGGTGGTTTTGAAATGGAGGAAGAAGGATGCTGTTCTGTGGTTTGGGTTGTCTCTGTTTCTGCTTTTTCCTCTTGCTACGTCACTGAACGATGAAGGGAAAGCCTTGATGTCAATCAAGGCCTCGTTCAGCAATGTAGCCAATGTTTTACTGGACTGGAATGATGCTCATGACGAAGATTTCTGTTCTTGGCGTGGAGTTTTCTGTGACAATGTCAGCCTCTCTGTGGCCTCCTTGAATTTGTCCAGCTTGAATCTGGGCGGGGAGATTTCGCCAGCCCTTGGAGATTTGGGGAACTTGGAATCCGTAGACTTGCAGGGGAATAAGCTAACTGGTCAAATCCCGGATGAGATTGGCAACTGTGCTTCTCTTATATATCTGGATTTGTCTGACAATAATCTGTCTGGAGACATACCCTTCTCGGTTTCAAAGCTTAAGAAGCTTGAGGTTTTGAATTTGAAGAACAATCAGTTGACTGGTCCTATCCCTATAACTCTTACCCAAATTCCAAACCTTAAAACTCTTGATCTTGCTCGGAACCAGCTCATTGGTGAGATACCAAGGCTAATATATTGGAATGAAGTTTTGCAGTACCTTGGATTGCGAGGCAACTCTTTGACTGGAGCACTGTCCCCTGATATGTGTCAATTAACTGGTCTGTGGTACTTTGATGTTAGAGGCAATAACCTGACTGGCCCGATCCCTGACAACATTGGCAACTGTACAAGCTTTGAGATCCTGGACATATCGTACAATCAGATCACTGGTGGGATTCCATATAACATTGGATTTTTACAAGTGGCTACCCTGTCGCTACAAGGGAATAGACTGACTGGAAAGATTCCTGAGGTTATTGGTTTAATGCAGGCTCTTGCTGTTTTGGATTTAAGTGAGAATGAACTTGTAGGGTCAATTCCACCGATACTAGGCAATTTATCATACACTGGGAAACTATACCTGCATGGTAACAAGTTGACTGGGACAATTCCTCCTGAGCTCGGCAATATGTCAAAGCTTAGCTATTTGCAATTAAATGACAACAATCTGGTTGGTAGAATTCCTGGTGAGCTTGGGAAGCTGAACCAGTTGTTTGAATTGAATCTTGCCAACAATGAACTGGAAGGTCCCATTCCACATGACATCGGTTCCTGCACTGCCTTGAATCAATTCAACGTTCATGGAAATCGCTTGACAGGATCCATTCCTTTAGCATTGAGAAATCTGCAGAGCTTGACCTATCTGAATTTGTCAGCAAATGCCTTCAGTGGTAGAATTAATCTTGAGTTGGGACACATCATCAATCTTGACACTTTGGATTTGTCCAGCAACAATTTCTCTGGCCCTGTTCCTGCTTCCGTTGGCAATCTTGAGCACCTCCTGACTTTGAATTTGAGTGGGAATCATCTTGATGGTCCTTTGCCTGCAGAATTTGGGGACCTCAGGAGTGTTCAAATCATTGATATGTCATTCAACAATCTCTCTGGGAGCATTCCCTCAGAATTGGGTCAGTTGCAGAACCTTGCGGCATTGTTGCTTAATAACAACAACTTGCGTGGGAAGATACCTGATCAGCTAACCAACTGTTTCAGCCTTGAGACCTTGAATTTTTCATACAACAATCTGTCAGGCATTGTACCTCCCATGAGGAACTTTTCACGTTTTTCACCAAACAGCTTCATTGCAAACCCGCTGTTGTGCAGTGACTGGTTGGGATCGATATGCAGACCCGTTGCCCCCAAGTCCAGGGCGGTATTCTCCCGAGCAGCAGTTGTCTGTATGGCGTTAGGCTTCATCACATTATTATCCATGGTAATAGTTGCGATATACAAGTCCAACCAACCAAAGCAGTTACTGATGGGATCTACCAAGAATGGACAAGGTCCTGCTAAACTTGTAATCCTTCACATGGATATGGCCATACACAGCTTTGATGATATAATGAGAATCACTGAGAATCTAAATGAGAAGTATATCATAGGGTATGGTGCTTCTAGCACAGTGTACAAGTGTGTACTGAAAAATTCCCGACCAATGGCAATCAAGCGACTCTACAACCGGTATCCACACAACTGGAGGGAGTTTGAGACGGAACTTGAAACCATTGGCAGTATCAAGCATAGAAATTTAGTCAGTTTGCATGGGTATTCACTGTCTCCCTTGGGCAATCTCCTGTTCTATGACTACATGGAGAACGGGTCTCTGTGGGATATGCTTCATGGACCATCGAAAAAGGTTAAACTTGATTGGGAAACACGTTTGAAAATAGCAGTGGGAGCAGCTCAAGGACTTGCTTATCTTCACCATGACTGCAACCCCCGCATTATTCATAGGGATGTGAAATCCTCTAATATCCTGCTGGATGAGAATTTTGAAGCTCATCTCTCTGATTTTGGAATTGCAAAATCCATCCCCAGTGCGGATACCCATGCATCAACTTATGTTCTGGGAACAATTGGTTACATTGATCCAGAATATGCTAGGACTTCTCGGCTCAATGAAAAATCTGATGTATATAGCTTTGGCATTGTGCTTCTTGAGCTACTGACTGGGAAGAAGGCTGTGGACAAGGAGTCTAACTTGCATCATCTGATATTGTCCAACGCGGATAACAATACTGTAATGGAGACTGTTGACCCTGAAGTTTCTGTCACCTGCATGGATATAACTCATGTCAAGAAAACTTTTCAACTCGCCCTGCTGTGCACCAAGCGAAACCCCACTGAGAGGCCAACAATGCATGAAGTTGCAAGGGTTTTGGTCTCCCTCCTACCTTTACATCACTCAAAGAAGCCTCTTTCAAAGGGTATCGACTATGATAAGTTTGCGGTTAACAAGGAACAACAACAACCAAAAGTGCAACAGCAGAAGCCTGTTCGGCAAGAAAGTCATTCATCTGATGCTCAATGGTTTCTTCGGTTTGGTGAGGTTATATCCAAGAACACACTCTAA

Protein Analysis

985

Amino Acids

108.71

Weight (kDa)

6.16

Isoelectric Point (pI)

27.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 32 - 73 6e-11 Leucine rich repeat N-terminal domain
LRR_4 PF12799 126 - 162 9e-07 Leucine Rich repeats (2 copies)
LRR_8 PF13855 130 - 185 2.3e-08 Leucine rich repeat
LRR_4 PF12799 149 - 190 3.2e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 320 - 376 7.5e-06 Leucine rich repeat
LRR_14 PF23598 335 - 445 3.5e-08 Leucine-rich repeat region
LRR_8 PF13855 437 - 496 3e-08 Leucine rich repeat
LRR_14 PF23598 455 - 539 4e-08 Leucine-rich repeat region
Pkinase PF00069 658 - 919 1e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 661 - 921 1.7e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07180 AT5G07180 AT5G62230 AT5G62230
fragaria_vesca FvH4_7g28290 FvH4_7g28290 FvH4_7g28290 FvH4_7g28290
malus_domestica MD02G1216900.v1.1 MD07G1259500.v1.1 MD17G1114200.v1.1 MD17G1114300.v1.1
prunus_persica Prupe.2G283600_v2.0.a1
pyrus_communis pycom05g04060 pycom07g23280 pycom15g04040
rosa_chinensis RchiOBHm_Chr1g0375261 RchiOBHm_Chr2g0167851 RchiOBHm_Chr4g0390421 RchiOBHm_Chr4g0390431 RchiOBHm_Chr4g0442901 RchiOBHm_Chr4g0442981 RchiOBHm_Chr7g0228991 RchiOBHm_Chr7g0239711
rosa_laevigata RLG00000005460 RLG00000005968 RLG00000005971 RLG00000005973 RLG00000005978 RLG00000008547 RLG00000010012 RLG00000010245 RLG00000026673 RLG00000030046 RLG00000031637
rosa_multiflora Rmu_co8166860.1_g000001 Rmu_co8410161.1_g000001 Rmu_sc0000554.1_g000034 Rmu_sc0001782.1_g000050 Rmu_sc0002096.1_g000036 Rmu_sc0003006.1_g000022 Rmu_sc0003701.1_g000003 Rmu_sc0005877.1_g000004 Rmu_sc0006329.1_g000003 Rmu_sc0006695.1_g000032 Rmu_sc0008108.1_g000003 Rmu_sc0009414.1_g000003 Rmu_sc0010356.1_g000001 Rmu_sc0014119.1_g000002 Rmu_sc0014119.1_g000003 Rmu_sc0014161.1_g000004 Rmu_sc0023009.1_g000001 Rmu_sc0036835.1_g000001 Rmu_ssc0000204.1_g000009
rosa_roxburghii Rroxscaffold_1G00024810 Rroxscaffold_2G00110800 Rroxscaffold_2G00122430 Rroxscaffold_3G00261280 Rroxscaffold_4G00282890 Rroxscaffold_4G00330910 Rroxscaffold_5G00370350 Rroxscaffold_7G00161820
rosa_rugosa Rorug01G0389600 Rorug01G0389700 Rorug01G0389800 Rorug01G0389900 Rorug01G0389900 Rorug01G0390000 Rorug04G0343700 Rorug07G0155300
rosa_samantha Rh1AG400300 Rh1CG377300 Rh1DG394700 Rh2AG367100 Rh2BG612000 Rh2CG350500 Rh2DG389600 Rh3CG058100 Rh4AG396700 Rh4AG397100 Rh4AG397500 Rh4AG397600 Rh4AG397700 Rh4BG003000 Rh4BG127700 Rh4BG370500 Rh4BG393400 Rh4BG409200 Rh4CG035400 Rh4CG166700 Rh4CG408200 Rh4CG424700 Rh4DG028000 Rh4DG028100 Rh4DG403300 Rh4DG403800 Rh6DG080900 Rh7DG202700
rosa_wichuraiana Rw1G035540 Rw4G032760 Rw4G034230 Rw4G034240 Rw4G034260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2669
Acc36I ACCTGC 2 cut(s) 975, 2669
AccB7I CCANNNNNTGG 1 cut(s) 290
AccI GTMKAC 1 cut(s) 318
AccII CGCG 1 cut(s) 2612
AciI CCGC 9 cut(s) 11, 270, 1532, 1697, 1757, 2326, 2426, 2612, 2834
AclI AACGTT 1 cut(s) 1182
AclWI GGATC 7 cut(s) 703, 739, 1200, 1213, 1729, 1876, 2468
AcoI YGGCCR 1 cut(s) 1923
AcuI CTGAAG 2 cut(s) 1255, 2667
AfaI GTAC 7 cut(s) 602, 674, 733, 761, 1644, 2012, 2022
AfiI CCNNNNNNNGG 9 cut(s) 235, 290, 935, 1007, 1752, 1915, 2041, 2425, 2733
AflII CTTAAG 1 cut(s) 443
AflIII ACRYGT 1 cut(s) 2261
AgeI ACCGGT 1 cut(s) 2064
AhdI GACNNNNNGTC 1 cut(s) 2578
AjnI CCWGG 3 cut(s) 747, 1069, 1751
AloI GAACNNNNNNTCC 2 cut(s) 296, 328
Alw21I GWGCWC 4 cut(s) 637, 1008, 1383, 2714
Alw26I GTCTC 8 cut(s) 66, 411, 1598, 2087, 2166, 2211, 2627, 2773
Alw44I GTGCAC 1 cut(s) 2710
AlwI GGATC 7 cut(s) 703, 739, 1200, 1213, 1729, 1876, 2468
AlwNI CAGNNNCTG 2 cut(s) 1713, 1861
Ama87I CYCGRG 1 cut(s) 1767
AoxI GGCC 6 cut(s) 129, 237, 703, 1348, 1923, 2735
ApaLI GTGCAC 1 cut(s) 2710
ApeKI GCWGC 2 cut(s) 1772, 2285
ArsI GACNNNNNNTTYG 2 cut(s) 1381, 1413
AseI ATTAAT 1 cut(s) 1284
AsiGI ACCGGT 1 cut(s) 2064
Asp700I GAANNNNTTC 1 cut(s) 1175
AspS9I GGNCC 8 cut(s) 488, 704, 1133, 1349, 1421, 1444, 1889, 2228
AsuC2I CCSGG 1 cut(s) 353
AsuHPI GGTGA 6 cut(s) 572, 1085, 1665, 2300, 2650, 2942
AvaI CYCGRG 1 cut(s) 1767
AvaII GGWCC 6 cut(s) 488, 1133, 1421, 1444, 1889, 2228
AxyI CCTNAGG 2 cut(s) 855, 1448
BaeGI GKGCMC 1 cut(s) 2714
BalI TGGCCA 1 cut(s) 1925
BamHI GGATCC 1 cut(s) 1205
BanII GRGCYC 1 cut(s) 1008
Bbv12I GWGCWC 4 cut(s) 637, 1008, 1383, 2714
BbvI GCAGC 2 cut(s) 1784, 2297
BccI CCATC 4 cut(s) 1412, 1858, 2239, 2423
BciT130I CCWGG 3 cut(s) 749, 1071, 1753
BciVI GTATCC 2 cut(s) 2079, 2422
BclI TGATCA 1 cut(s) 1576
BcnI CCSGG 1 cut(s) 353
BcoDI GTCTC 8 cut(s) 66, 411, 1598, 2087, 2166, 2211, 2627, 2773
BfaI CTAG 3 cut(s) 935, 2001, 2487
BfmI CTRYAG 2 cut(s) 1232, 1431
BfrI CTTAAG 1 cut(s) 443
BfuAI ACCTGC 2 cut(s) 975, 2669
BfuI GTATCC 2 cut(s) 2079, 2422
BglI GCCNNNNNGGC 1 cut(s) 2884
BisI GCNGC 3 cut(s) 1533, 1773, 2286
BlpI GCTNAGC 1 cut(s) 1025
BlsI GCNGC 3 cut(s) 1534, 1774, 2287
Bme1390I CCNGG 4 cut(s) 353, 749, 1071, 1753
Bme18I GGWCC 6 cut(s) 488, 1133, 1421, 1444, 1889, 2228
BmeRI GACNNNNNGTC 1 cut(s) 2578
BmeT110I CYCGRG 1 cut(s) 1767
BmgT120I GGNCC 8 cut(s) 488, 704, 1133, 1349, 1421, 1444, 1889, 2228
BmiI GGNNCC 5 cut(s) 548, 1135, 1156, 1207, 1445
BmrFI CCNGG 4 cut(s) 353, 749, 1071, 1753
BmrI ACTGGG 4 cut(s) 963, 996, 2415, 2565
BmsI GCATC 5 cut(s) 28, 163, 2447, 2599, 2896
BmuI ACTGGG 4 cut(s) 963, 996, 2415, 2565
BpmI CTGGAG 3 cut(s) 435, 651, 2101
Bpu10I CCTNAGC 1 cut(s) 1002
Bpu1102I GCTNAGC 1 cut(s) 1025
BpuEI CTTGAG 6 cut(s) 473, 1310, 1397, 1622, 2274, 2564
BpuMI CCSGG 1 cut(s) 353
Bsa29I ATCGAT 1 cut(s) 1724
BsaBI GATNNNNATC 1 cut(s) 122
BsaI GGTCTC 3 cut(s) 1598, 2211, 2773
BsaJI CCNNGG 6 cut(s) 289, 569, 604, 1752, 1815, 2166
BsaWI WCCGGW 1 cut(s) 2064
BsaXI ACNNNNNCTCC 4 cut(s) 982, 1012, 2189, 2219
Bsc4I CCNNNNNNNGG 9 cut(s) 235, 290, 935, 1007, 1752, 1915, 2041, 2425, 2733
Bse118I RCCGGY 1 cut(s) 2064
Bse21I CCTNAGG 2 cut(s) 855, 1448
Bse3DI GCAATG 2 cut(s) 148, 1686
Bse8I GATNNNNATC 1 cut(s) 122
BseBI CCWGG 3 cut(s) 749, 1071, 1753
BseCI ATCGAT 1 cut(s) 1724
BseDI CCNNGG 6 cut(s) 289, 569, 604, 1752, 1815, 2166
BseGI GGATG 5 cut(s) 43, 361, 2347, 2374, 2415
BseJI GATNNNNATC 1 cut(s) 122
BseLI CCNNNNNNNGG 9 cut(s) 235, 290, 935, 1007, 1752, 1915, 2041, 2425, 2733
BseMI GCAATG 2 cut(s) 148, 1686
BseMII CTCAG 6 cut(s) 846, 993, 1462, 1518, 1950, 2721
BseRI GAGGAG 1 cut(s) 20
BseSI GKGCMC 1 cut(s) 2714
BseXI GCAGC 2 cut(s) 1784, 2297
BsgI GTGCAG 2 cut(s) 1144, 1726
Bsh1236I CGCG 1 cut(s) 2612
BshFI GGCC 6 cut(s) 131, 239, 705, 1350, 1925, 2737
BshTI ACCGGT 1 cut(s) 2064
BshVI ATCGAT 1 cut(s) 1724
BsiHKAI GWGCWC 4 cut(s) 637, 1008, 1383, 2714
BsiHKCI CYCGRG 1 cut(s) 1767
BsiSI CCGG 2 cut(s) 353, 2065
BslFI GGGAC 5 cut(s) 625, 1003, 1119, 1311, 1457
BslI CCNNNNNNNGG 9 cut(s) 235, 290, 935, 1007, 1752, 1915, 2041, 2425, 2733
BsmAI GTCTC 8 cut(s) 66, 411, 1598, 2087, 2166, 2211, 2627, 2773
BsmBI CGTCTC 1 cut(s) 2087
BsmFI GGGAC 5 cut(s) 625, 1003, 1119, 1311, 1457
BsmI GAATGC 1 cut(s) 1497
BsnI GGCC 6 cut(s) 131, 239, 705, 1350, 1925, 2737
Bso31I GGTCTC 3 cut(s) 1598, 2211, 2773
BsoBI CYCGRG 1 cut(s) 1767
Bsp1286I GDGCHC 4 cut(s) 637, 1008, 1383, 2714
Bsp1407I TGTACA 2 cut(s) 731, 2010
Bsp143I GATC 9 cut(s) 535, 708, 744, 768, 1205, 1576, 1721, 1868, 2473
Bsp1720I GCTNAGC 1 cut(s) 1025
Bsp19I CCATGG 1 cut(s) 1815
BspACI CCGC 9 cut(s) 11, 270, 1532, 1697, 1757, 2326, 2426, 2612, 2834
BspANI GGCC 6 cut(s) 131, 239, 705, 1350, 1925, 2737
BspCNI CTCAG 6 cut(s) 847, 994, 1461, 1517, 1951, 2722
BspDI ATCGAT 1 cut(s) 1724
BspFNI CGCG 1 cut(s) 2612
BspHI TCATGA 1 cut(s) 178
BspLI GGNNCC 5 cut(s) 548, 1135, 1156, 1207, 1445
BspMAI CTGCAG 2 cut(s) 1236, 1435
BspMI ACCTGC 2 cut(s) 975, 2669
BspPI GGATC 7 cut(s) 703, 739, 1200, 1213, 1729, 1876, 2468
BspTI CTTAAG 1 cut(s) 443
BspTNI GGTCTC 3 cut(s) 1598, 2211, 2773
BsrDI GCAATG 2 cut(s) 148, 1686
BsrFI RCCGGY 1 cut(s) 2064
BsrGI TGTACA 2 cut(s) 731, 2010
BssAI RCCGGY 1 cut(s) 2064
BssECI CCNNGG 6 cut(s) 289, 569, 604, 1752, 1815, 2166
BssMI GATC 9 cut(s) 535, 708, 744, 768, 1205, 1576, 1721, 1868, 2473
BssT1I CCWWGG 5 cut(s) 289, 569, 604, 1815, 2166
Bst2UI CCWGG 3 cut(s) 749, 1071, 1753
Bst4CI ACNGT 8 cut(s) 371, 639, 731, 1592, 2008, 2160, 2626, 2638
BstAFI CTTAAG 1 cut(s) 443
BstAUI TGTACA 2 cut(s) 731, 2010
BstC8I GCNNGC 3 cut(s) 285, 876, 1431
BstDEI CTNAG 7 cut(s) 855, 1002, 1025, 1448, 1504, 1959, 2730
BstDSI CCRYGG 1 cut(s) 1815
BstF5I GGATG 5 cut(s) 43, 361, 2347, 2374, 2415
BstFNI CGCG 1 cut(s) 2612
BstKTI GATC 9 cut(s) 538, 711, 747, 771, 1208, 1579, 1724, 1871, 2476
BstMAI GTCTC 8 cut(s) 66, 411, 1598, 2087, 2166, 2211, 2627, 2773
BstMBI GATC 9 cut(s) 535, 708, 744, 768, 1205, 1576, 1721, 1868, 2473
BstMWI GCNNNNNNNGC 7 cut(s) 448, 1084, 2282, 2325, 2545, 2743, 2884
BstNI CCWGG 3 cut(s) 749, 1071, 1753
BstSCI CCNGG 4 cut(s) 351, 747, 1069, 1751
BstSFI CTRYAG 2 cut(s) 1232, 1431
BstSLI GKGCMC 1 cut(s) 2714
BstUI CGCG 1 cut(s) 2612
BstV1I GCAGC 2 cut(s) 1784, 2297
BstX2I RGATCY 3 cut(s) 744, 1205, 1868
BstXI CCANNNNNNTGG 2 cut(s) 557, 2079
BstYI RGATCY 3 cut(s) 744, 1205, 1868
Bsu15I ATCGAT 1 cut(s) 1724
Bsu36I CCTNAGG 2 cut(s) 855, 1448
BsuI GTATCC 2 cut(s) 2079, 2422
BsuRI GGCC 6 cut(s) 131, 239, 705, 1350, 1925, 2737
BsuTUI ATCGAT 1 cut(s) 1724
BtgI CCRYGG 1 cut(s) 1815
BtsCI GGATG 5 cut(s) 43, 361, 2347, 2374, 2415
BtsI GCAGTG 3 cut(s) 1161, 1714, 2283
BveI ACCTGC 2 cut(s) 975, 2669
Cac8I GCNNGC 3 cut(s) 285, 876, 1431
CaiI CAGNNNCTG 2 cut(s) 1713, 1861
CciI TCATGA 1 cut(s) 178
Cfr10I RCCGGY 1 cut(s) 2064
Cfr13I GGNCC 8 cut(s) 488, 704, 1133, 1349, 1421, 1444, 1889, 2228
ClaI ATCGAT 1 cut(s) 1724
Csp6I GTAC 7 cut(s) 601, 673, 732, 760, 1643, 2011, 2021
CspAI ACCGGT 1 cut(s) 2064
CspCI CAANNNNNGTGG 2 cut(s) 1543, 1578
CviQI GTAC 7 cut(s) 601, 673, 732, 760, 1643, 2011, 2021
DdeI CTNAG 7 cut(s) 855, 1002, 1025, 1448, 1504, 1959, 2730
DpnI GATC 9 cut(s) 537, 710, 746, 770, 1207, 1578, 1723, 1870, 2475
DpnII GATC 9 cut(s) 535, 708, 744, 768, 1205, 1576, 1721, 1868, 2473
DriI GACNNNNNGTC 1 cut(s) 2578
EaeI YGGCCR 1 cut(s) 1923
Eam1105I GACNNNNNGTC 1 cut(s) 2578
Ecl136II GAGCTC 1 cut(s) 1006
Eco130I CCWWGG 5 cut(s) 289, 569, 604, 1815, 2166
Eco147I AGGCCT 1 cut(s) 131
Eco24I GRGCYC 1 cut(s) 1008
Eco31I GGTCTC 3 cut(s) 1598, 2211, 2773
Eco47I GGWCC 6 cut(s) 488, 1133, 1421, 1444, 1889, 2228
Eco53kI GAGCTC 1 cut(s) 1006
Eco57I CTGAAG 2 cut(s) 1255, 2667
Eco81I CCTNAGG 2 cut(s) 855, 1448
Eco88I CYCGRG 1 cut(s) 1767
EcoICRI GAGCTC 1 cut(s) 1006
EcoO109I RGGNCCY 3 cut(s) 1133, 1444, 1889
EcoRI GAATTC 1 cut(s) 1065
EcoRII CCWGG 3 cut(s) 747, 1069, 1751
EcoT14I CCWWGG 5 cut(s) 289, 569, 604, 1815, 2166
EcoT22I ATGCAT 2 cut(s) 2440, 2748
EcoT38I GRGCYC 1 cut(s) 1008
ErhI CCWWGG 5 cut(s) 289, 569, 604, 1815, 2166
Esp3I CGTCTC 1 cut(s) 2087
FaqI GGGAC 5 cut(s) 625, 1003, 1119, 1311, 1457
FauI CCCGC 3 cut(s) 263, 1704, 2333
FbaI TGATCA 1 cut(s) 1576
FblI GTMKAC 1 cut(s) 318
Fnu4HI GCNGC 3 cut(s) 1533, 1773, 2286
FokI GGATG 5 cut(s) 50, 368, 2354, 2381, 2402
FriOI GRGCYC 1 cut(s) 1008
Fsp4HI GCNGC 3 cut(s) 1533, 1773, 2286
FspBI CTAG 3 cut(s) 935, 2001, 2487
GluI GCNGC 3 cut(s) 1533, 1773, 2286
GsuI CTGGAG 3 cut(s) 435, 651, 2101
HaeIII GGCC 6 cut(s) 131, 239, 705, 1350, 1925, 2737
HapII CCGG 2 cut(s) 353, 2065
HincII GTYRAC 4 cut(s) 483, 984, 2641, 2839
HindII GTYRAC 4 cut(s) 483, 984, 2641, 2839
HindIII AAGCTT 4 cut(s) 440, 449, 736, 1022
HpaI GTTAAC 1 cut(s) 2839
HpaII CCGG 2 cut(s) 353, 2065
HphI GGTGA 6 cut(s) 572, 1085, 1665, 2300, 2650, 2942
Hpy166II GTNNAC 9 cut(s) 319, 483, 984, 2011, 2021, 2572, 2641, 2712, 2839
Hpy188I TCNGA 9 cut(s) 400, 546, 768, 1251, 1507, 2395, 2515, 2598, 2905
Hpy8I GTNNAC 9 cut(s) 319, 483, 984, 2011, 2021, 2572, 2641, 2712, 2839
HpyAV CCTTC 7 cut(s) 28, 98, 436, 1124, 1279, 1919, 2557
HpyCH4III ACNGT 8 cut(s) 371, 639, 731, 1592, 2008, 2160, 2626, 2638
HpyCH4IV ACGT 4 cut(s) 89, 1182, 1666, 2263
HpyF10VI GCNNNNNNNGC 7 cut(s) 448, 1084, 2282, 2325, 2545, 2743, 2884
HpyF3I CTNAG 7 cut(s) 855, 1002, 1025, 1448, 1504, 1959, 2730
HpySE526I ACGT 4 cut(s) 89, 1182, 1666, 2263
Ksp22I TGATCA 1 cut(s) 1576
KspAI GTTAAC 1 cut(s) 2839
Kzo9I GATC 9 cut(s) 535, 708, 744, 768, 1205, 1576, 1721, 1868, 2473
LmnI GCTCC 3 cut(s) 632, 1494, 2282
Lsp1109I GCAGC 2 cut(s) 1784, 2297
LweI GCATC 5 cut(s) 28, 163, 2447, 2599, 2896
MaeI CTAG 3 cut(s) 935, 2001, 2487
MaeII ACGT 4 cut(s) 89, 1182, 1666, 2263
MaeIII GTNAC 7 cut(s) 90, 215, 974, 1709, 1857, 2465, 2656
MalI GATC 9 cut(s) 537, 710, 746, 770, 1207, 1578, 1723, 1870, 2475
MboI GATC 9 cut(s) 535, 708, 744, 768, 1205, 1576, 1721, 1868, 2473
MboII GAAGA 7 cut(s) 43, 197, 481, 1579, 2297, 2572, 2912
MfeI CAATTG 1 cut(s) 2460
MflI RGATCY 3 cut(s) 744, 1205, 1868
MhlI GDGCHC 4 cut(s) 637, 1008, 1383, 2714
MlsI TGGCCA 1 cut(s) 1925
MluNI TGGCCA 1 cut(s) 1925
MlyI GAGTC 2 cut(s) 2049, 2588
MmeI TCCRAC 2 cut(s) 1866, 2631
Mox20I TGGCCA 1 cut(s) 1925
Mph1103I ATGCAT 2 cut(s) 2440, 2748
MroXI GAANNNNTTC 1 cut(s) 1175
MscI TGGCCA 1 cut(s) 1925
MslI CAYNNNNRTG 5 cut(s) 1416, 1469, 1919, 2340, 2663
Msp20I TGGCCA 1 cut(s) 1925
MspA1I CMGCKG 1 cut(s) 1699
MspCI CTTAAG 1 cut(s) 443
MspI CCGG 2 cut(s) 353, 2065
MspR9I CCNGG 4 cut(s) 353, 749, 1071, 1753
MunI CAATTG 1 cut(s) 2460
Mva1269I GAATGC 1 cut(s) 1497
MvaI CCWGG 3 cut(s) 749, 1071, 1753
MvnI CGCG 1 cut(s) 2612
MwoI GCNNNNNNNGC 7 cut(s) 448, 1084, 2282, 2325, 2545, 2743, 2884
NciI CCSGG 1 cut(s) 353
NcoI CCATGG 1 cut(s) 1815
NdeII GATC 9 cut(s) 535, 708, 744, 768, 1205, 1576, 1721, 1868, 2473
NlaIV GGNNCC 5 cut(s) 548, 1135, 1156, 1207, 1445
NmeAIII GCCGAG 2 cut(s) 987, 2476
NmuCI GTSAC 4 cut(s) 90, 215, 1709, 2656
NsiI ATGCAT 2 cut(s) 2440, 2748
PagI TCATGA 1 cut(s) 178
PaqCI CACCTGC 1 cut(s) 2669
PceI AGGCCT 1 cut(s) 131
PctI GAATGC 1 cut(s) 1497
PdmI GAANNNNTTC 1 cut(s) 1175
PfeI GAWTC 9 cut(s) 262, 311, 781, 850, 1108, 1171, 1408, 1953, 1963
PflFI GACNNNGTC 2 cut(s) 221, 1887
PflMI CCANNNNNTGG 1 cut(s) 290
PfoI TCCNGGA 2 cut(s) 351, 747
PinAI ACCGGT 1 cut(s) 2064
PkrI GCNGC 3 cut(s) 1534, 1774, 2287
PleI GAGTC 2 cut(s) 2049, 2587
PpsI GAGTC 2 cut(s) 2049, 2587
PpuMI RGGWCCY 3 cut(s) 1133, 1444, 1889
PshBI ATTAAT 1 cut(s) 1284
Psp124BI GAGCTC 1 cut(s) 1008
Psp1406I AACGTT 1 cut(s) 1182
Psp5II RGGWCCY 3 cut(s) 1133, 1444, 1889
Psp6I CCWGG 3 cut(s) 747, 1069, 1751
PspGI CCWGG 3 cut(s) 747, 1069, 1751
PspN4I GGNNCC 5 cut(s) 548, 1135, 1156, 1207, 1445
PspPI GGNCC 8 cut(s) 488, 704, 1133, 1349, 1421, 1444, 1889, 2228
PspPPI RGGWCCY 3 cut(s) 1133, 1444, 1889
PstI CTGCAG 2 cut(s) 1236, 1435
PstNI CAGNNNCTG 2 cut(s) 1713, 1861
PsuI RGATCY 3 cut(s) 744, 1205, 1868
PsyI GACNNNGTC 2 cut(s) 221, 1887
RsaI GTAC 7 cut(s) 602, 674, 733, 761, 1644, 2012, 2022
RsaNI GTAC 7 cut(s) 601, 673, 732, 760, 1643, 2011, 2021
RseI CAYNNNNRTG 5 cut(s) 1416, 1469, 1919, 2340, 2663
SacI GAGCTC 1 cut(s) 1008
SatI GCNGC 3 cut(s) 1533, 1773, 2286
Sau3AI GATC 9 cut(s) 535, 708, 744, 768, 1205, 1576, 1721, 1868, 2473
Sau96I GGNCC 8 cut(s) 488, 704, 1133, 1349, 1421, 1444, 1889, 2228
SchI GAGTC 2 cut(s) 2049, 2588
ScrFI CCNGG 4 cut(s) 353, 749, 1071, 1753
SduI GDGCHC 4 cut(s) 637, 1008, 1383, 2714
SfaNI GCATC 5 cut(s) 28, 163, 2447, 2599, 2896
SfcI CTRYAG 2 cut(s) 1232, 1431
SinI GGWCC 6 cut(s) 488, 1133, 1421, 1444, 1889, 2228
SmiMI CAYNNNNRTG 5 cut(s) 1416, 1469, 1919, 2340, 2663
SmlI CTYRAG 7 cut(s) 443, 452, 1289, 1376, 1601, 2289, 2543
SmoI CTYRAG 7 cut(s) 443, 452, 1289, 1376, 1601, 2289, 2543
SseBI AGGCCT 1 cut(s) 131
SsiI CCGC 9 cut(s) 11, 270, 1532, 1697, 1757, 2326, 2426, 2612, 2834
SspMI CTAG 3 cut(s) 935, 2001, 2487
SstI GAGCTC 1 cut(s) 1008
StuI AGGCCT 1 cut(s) 131
StyD4I CCNGG 4 cut(s) 351, 747, 1069, 1751
StyI CCWWGG 5 cut(s) 289, 569, 604, 1815, 2166
TaaI ACNGT 8 cut(s) 371, 639, 731, 1592, 2008, 2160, 2626, 2638
TaiI ACGT 4 cut(s) 92, 1185, 1669, 2266
TaqI TCGA 3 cut(s) 1724, 2234, 2817
TatI WGTACW 3 cut(s) 731, 2010, 2020
TauI GCSGC 1 cut(s) 1535
TfiI GAWTC 9 cut(s) 262, 311, 781, 850, 1108, 1171, 1408, 1953, 1963
TseFI GTSAC 4 cut(s) 90, 215, 1709, 2656
TseI GCWGC 2 cut(s) 1772, 2285
Tsp45I GTSAC 4 cut(s) 90, 215, 1709, 2656
TspGWI ACGGA 3 cut(s) 304, 1354, 2111
Tth111I GACNNNGTC 2 cut(s) 221, 1887
Van91I CCANNNNNTGG 1 cut(s) 290
Vha464I CTTAAG 1 cut(s) 443
VneI GTGCAC 1 cut(s) 2710
VpaK11BI GGWCC 6 cut(s) 488, 1133, 1421, 1444, 1889, 2228
VspI ATTAAT 1 cut(s) 1284
XcmI CCANNNNNNNNNTGG 3 cut(s) 157, 262, 1123
XmiI GTMKAC 1 cut(s) 318
XmnI GAANNNNTTC 1 cut(s) 1175
XspI CTAG 3 cut(s) 935, 2001, 2487
Zsp2I ATGCAT 2 cut(s) 2440, 2748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.