RLG00000026673
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000383
Physical Location & Seq
Forward (+)
4799772 .. 4806850
7079 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026673

Sequence Viewer

Length: 2751 bp
ATGGCAACTCTCTCTTCCACTTTTCGGTCCTTCAAAGTTGAAACCAAACCGGACTGCACTGTCACTCTCACGGCGCGTCCCCCGGCACGCGCCGATGGTGATAGTGGTGACAAAGGACGGGGCAGATTGGTGTCGAAAAAGCTGAGAGCTTTGGAAAACGTCCAGGTACGAATGACGGCCGTGCCCACATTTCACTCCTCCCCCCTCCCCACATTTTCTCTTCTTTCTTGGAAAGCCTTGATGTCAATCAAGGCCTCGAATCTGTCCAGCTTGAATCTGGGCGGGGAGATTTCTCCAGCCCTTGGAGATTTGGGGAACTTGGAATCCATAGACTTGCAGGGGAATAAGCTAACTGGTCAAATCCCGGATGAGATTGGCAGCTGTGCTTCTCTTATCTATCTGGATTTGTCTGACAATAATCTGTCTGGAGACATACCCTTCTCGGTTTCAAAGCTTAAGAAGCTTGAGGTTTTGAATTTGAAGAACAATCAGTTGAGTGGTCCCATCCCTACAACTCTTACCCAAATTCCAAACCTTAAAACTCTTGATGTTAGAGGCAATAACCTGACTGGCCCGATCCCTGACAACATTGGCAACTGTACAAGCTTTGAGATCCTGGACATATCATACAATCAGATCACTGGTGGGATTCCATATAACATTGGATTTTTACAAGTGGCTACCCTGTCGCTACAAGGGAATAGACTGACTGGAAAGATTCCCGAGGTTATTGGTTTAATGCAGGCTCTTGCTGTTTTGGATTTAAGTGAGAATGAACTTGTAGGGCCGATTCCACCGATACTAGGCAATTTATCATACACTGGGAAACTATACCTGCATGGTAACAAGTTGACTGGGACAATTCCTCCTGAGCTCGGCAATATGTCAAAGCTTAGCTATTTGCAATTAAATGACAACAATCTGGTTGGTAGAATTCCTGGTGAGCTTGGGAAGCTGAACCAGTTGTTTGAATTGAATCTTGCCAACAATGATCTGGAAGGTCCCATCCCACATGACATCGGTTCCTGCACTGCCTTGAATCAATTCAACGTTCATGGAAATCGCTTGACAGGATCCATTCCTTTACCATTGAGAAATCTGCAGAGCTTGACCTATCTGAATTTGTCAGCAAATGCCTTCAGTGGTAGAATTAATCTCGAGTTGGGACACATCATCAATCTTGACACTCTGGATTTGTCCAGCAACAATTTCTCTGGCCCTGTACCTGCTTCCGTTGGCAATCTTGAGCACCTCCTGACTTTGAATTTGAGTGGGAATCATCTTGATGGTCCTTTGCCTGCAGAATTTGGAGACCTCAGGAGTGTTCAAATCATGTTGCTTAATAACAACAACTTGCGTGGGAAGATACCTGATCAGCTAACCAACTGTTTCAGCCTTGAGACCTTGAATTTTTCATACAACAATCTGTCAGGCATTGTACCTCCCATGAGGAACTTTTCACGGTTTTCACCAAACAGCTTCATTGCAAACCCGCTGTTGTGCAGTGACTGGTTGGGATCGATATGCAGACCCGTTGCCCCCAAGTCCAGGGCTGTATTCTCCCGAGCTGCAGTTGTCTGTATGGCGTTAGGCTTCATCACATTATTATCCATGGTAATAGTTGCGATTTACAAGTCCAACCAACCAAAGCAGTTAATGATGGGATCTACCAAGAATGGACAAGGTCCTGCTAAACTTGTAATTCTTCACATGGATATGGCTATACACAGCTTTGATGATATAATGAGAATCACTGAGAATCTAAATGAGAAGTATATCATAGGGTATGGTGCTTCTAGCACAGTGTACAAGTGTGTACTGAAAAATTCCCGACCAATGGCAATCAAGCGACTCTACAACCGGTATCCACACAACTGGAGGGAGTTTGAGACGGAACTTGAAACCATTGGCAGTATCAAGCATAGAAATCTAGTCAGTTTGCATGGGTATTCCCTGTCTCCCCTGGGCAATCTCCTCTTCTATGACTACATGGAGAACGGGTCTCTGTGGGATATGCTTCATGGACCATCGAAAAAGGTTAAACTTGATTGGGAAACACGTTTGAAAATAGCAGTTGGAGCAGCTCAAGGACTTGCTTATCTTCATCATGACTGCAACCCCCGCATTATTCACAGGGATGTGAAATCGTCTAATATCCTGCTGGATGAGAATTTTGAAGCTCATCTCTCTGATTTTGGAATTGCAAAATCCATCCCCAGTGCAGATACCCATGCATCGACTTATGTTCTGGGAACAATTGGTTACATCGATCCAGAATATGCTAGGACTTCTCGGCTCAATGAAAAATCTGATGTATATAGCTTTGGCATTGTGCTTCTTGAGCTTCTGACTGGGAAGAAGGCTGTGGACAAGGAGTCTAACTTGCATCATCTGATATTGTCCAAGGCGGATAACAATACTGTAATGGAGACTGTTGACCCTGAAGTTTCTGTCACCTGCATGGATATAACTCATGTCAAGAAAACTTTTCAACTCGCCCTGCTATGCACCAAGCGAAACCCCACTGAGAGGCCAACAATGCATGAAGTTGCAAGGGTTTTGGTCTCCCTCCTACCTTTACATCACTCAAAGAAGACTCTTTCAAAGGGTATCGACTATGATAAGTTTGCGGTTAACAAGGAACAACAACCAAAAGTGCAACAGCAGAAGCCTGTTCGGCAAGAAAGTCATTCATCTGATGCCCAATGGTTTCTTCGGTTTGGTGAGGTTATATCCAAGAACACCCTCTAA

Protein Analysis

917

Amino Acids

100.78

Weight (kDa)

8.39

Isoelectric Point (pI)

29.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 99 - 208 5.3e-08 Leucine-rich repeat region
LRR_8 PF13855 106 - 165 8.9e-08 Leucine rich repeat
LRR_4 PF12799 130 - 166 4e-07 Leucine Rich repeats (2 copies)
LRR_8 PF13855 134 - 189 7.5e-08 Leucine rich repeat
LRR_8 PF13855 276 - 332 2e-06 Leucine rich repeat
LRR_14 PF23598 291 - 401 8.4e-08 Leucine-rich repeat region
LRR_14 PF23598 388 - 471 5.9e-06 Leucine-rich repeat region
LRR_8 PF13855 393 - 452 5.1e-07 Leucine rich repeat
Pkinase PF00069 590 - 851 8.3e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 593 - 853 1.5e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07180 AT5G07180 AT5G62230 AT5G62230
fragaria_vesca FvH4_7g28290 FvH4_7g28290 FvH4_7g28290 FvH4_7g28290
malus_domestica MD02G1216900.v1.1 MD07G1259500.v1.1 MD17G1114200.v1.1 MD17G1114300.v1.1
prunus_persica Prupe.2G283600_v2.0.a1
pyrus_communis pycom05g04060 pycom07g23280 pycom15g04040
rosa_chinensis RchiOBHm_Chr1g0375261 RchiOBHm_Chr2g0167851 RchiOBHm_Chr4g0390421 RchiOBHm_Chr4g0390431 RchiOBHm_Chr4g0442901 RchiOBHm_Chr4g0442981 RchiOBHm_Chr7g0228991 RchiOBHm_Chr7g0239711
rosa_laevigata RLG00000005460 RLG00000005968 RLG00000005971 RLG00000005973 RLG00000005978 RLG00000008547 RLG00000010012 RLG00000010245 RLG00000026673 RLG00000030046 RLG00000031637
rosa_multiflora Rmu_co8166860.1_g000001 Rmu_co8410161.1_g000001 Rmu_sc0000554.1_g000034 Rmu_sc0001782.1_g000050 Rmu_sc0002096.1_g000036 Rmu_sc0003006.1_g000022 Rmu_sc0003701.1_g000003 Rmu_sc0005877.1_g000004 Rmu_sc0006329.1_g000003 Rmu_sc0006695.1_g000032 Rmu_sc0008108.1_g000003 Rmu_sc0009414.1_g000003 Rmu_sc0010356.1_g000001 Rmu_sc0014119.1_g000002 Rmu_sc0014119.1_g000003 Rmu_sc0014161.1_g000004 Rmu_sc0023009.1_g000001 Rmu_sc0036835.1_g000001 Rmu_ssc0000204.1_g000009
rosa_roxburghii Rroxscaffold_1G00024810 Rroxscaffold_2G00110800 Rroxscaffold_2G00122430 Rroxscaffold_3G00261280 Rroxscaffold_4G00282890 Rroxscaffold_4G00330910 Rroxscaffold_5G00370350 Rroxscaffold_7G00161820
rosa_rugosa Rorug01G0389600 Rorug01G0389700 Rorug01G0389800 Rorug01G0389900 Rorug01G0389900 Rorug01G0390000 Rorug04G0343700 Rorug07G0155300
rosa_samantha Rh1AG400300 Rh1CG377300 Rh1DG394700 Rh2AG367100 Rh2BG612000 Rh2CG350500 Rh2DG389600 Rh3CG058100 Rh4AG396700 Rh4AG397100 Rh4AG397500 Rh4AG397600 Rh4AG397700 Rh4BG003000 Rh4BG127700 Rh4BG370500 Rh4BG393400 Rh4BG409200 Rh4CG035400 Rh4CG166700 Rh4CG408200 Rh4CG424700 Rh4DG028000 Rh4DG028100 Rh4DG403300 Rh4DG403800 Rh6DG080900 Rh7DG202700
rosa_wichuraiana Rw1G035540 Rw4G032760 Rw4G034230 Rw4G034240 Rw4G034260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2465
AasI GACNNNNNNGTC 1 cut(s) 59
Acc36I ACCTGC 3 cut(s) 843, 1234, 2465
AccB7I CCANNNNNTGG 1 cut(s) 302
AccII CGCG 2 cut(s) 76, 90
AciI CCGC 5 cut(s) 282, 1493, 2122, 2408, 2630
AclI AACGTT 1 cut(s) 1050
AclWI GGATC 7 cut(s) 571, 607, 1068, 1081, 1525, 1672, 2264
AcoI YGGCCR 1 cut(s) 177
AcsI RAATTY 9 cut(s) 475, 525, 933, 1120, 1264, 1304, 1408, 1825, 2170
AcuI CTGAAG 2 cut(s) 1123, 2463
AfaI GTAC 6 cut(s) 168, 601, 1224, 1440, 1808, 1818
AfiI CCNNNNNNNGG 7 cut(s) 24, 302, 803, 875, 1548, 1837, 2529
AflII CTTAAG 1 cut(s) 455
AflIII ACRYGT 1 cut(s) 2057
AgeI ACCGGT 1 cut(s) 1860
AhdI GACNNNNNGTC 1 cut(s) 2374
AjnI CCWGG 5 cut(s) 162, 615, 937, 1547, 1962
AjuI GAANNNNNNNTTGG 2 cut(s) 2697, 2729
AloI GAACNNNNNNTCC 2 cut(s) 308, 340
Alw21I GWGCWC 2 cut(s) 876, 1251
Alw26I GTCTC 8 cut(s) 423, 1305, 1394, 1883, 1962, 2007, 2423, 2569
AlwI GGATC 7 cut(s) 571, 607, 1068, 1081, 1525, 1672, 2264
AlwNI CAGNNNCTG 1 cut(s) 1509
Ama87I CYCGRG 3 cut(s) 722, 1157, 1563
AoxI GGCC 6 cut(s) 177, 252, 571, 785, 1216, 2531
ApeKI GCWGC 3 cut(s) 378, 1568, 2081
ApoI RAATTY 9 cut(s) 475, 525, 933, 1120, 1264, 1304, 1408, 1825, 2170
ArsI GACNNNNNNTTYG 2 cut(s) 1249, 1281
AseI ATTAAT 1 cut(s) 1152
AsiGI ACCGGT 1 cut(s) 1860
Asp700I GAANNNNTTC 1 cut(s) 1043
AspLEI GCGC 2 cut(s) 76, 92
AspS9I GGNCC 9 cut(s) 27, 500, 572, 785, 1001, 1217, 1289, 1685, 2024
AsuC2I CCSGG 2 cut(s) 83, 365
AsuHPI GGTGA 6 cut(s) 110, 119, 953, 1461, 2446, 2735
AvaI CYCGRG 3 cut(s) 722, 1157, 1563
AvaII GGWCC 6 cut(s) 27, 500, 1001, 1289, 1685, 2024
AxyI CCTNAGG 1 cut(s) 1316
BaeGI GKGCMC 1 cut(s) 186
BamHI GGATCC 1 cut(s) 1073
BanII GRGCYC 1 cut(s) 876
BbsI GAAGAC 1 cut(s) 2600
Bbv12I GWGCWC 2 cut(s) 876, 1251
BbvI GCAGC 3 cut(s) 390, 1555, 2093
BccI CCATC 7 cut(s) 89, 512, 1013, 1280, 1654, 2035, 2219
BceAI ACGGC 3 cut(s) 87, 164, 192
BciT130I CCWGG 5 cut(s) 164, 617, 939, 1549, 1964
BciVI GTATCC 1 cut(s) 1875
BclI TGATCA 1 cut(s) 1372
BcnI CCSGG 2 cut(s) 83, 365
BcoDI GTCTC 8 cut(s) 423, 1305, 1394, 1883, 1962, 2007, 2423, 2569
BfaI CTAG 4 cut(s) 803, 1797, 1931, 2283
BfmI CTRYAG 3 cut(s) 1100, 1299, 1569
BfrI CTTAAG 1 cut(s) 455
BfuAI ACCTGC 3 cut(s) 843, 1234, 2465
BfuI GTATCC 1 cut(s) 1875
BglI GCCNNNNNGGC 1 cut(s) 2677
BisI GCNGC 3 cut(s) 379, 1569, 2082
BlpI GCTNAGC 1 cut(s) 893
BlsI GCNGC 3 cut(s) 380, 1570, 2083
Bme1390I CCNGG 7 cut(s) 83, 164, 365, 617, 939, 1549, 1964
Bme18I GGWCC 6 cut(s) 27, 500, 1001, 1289, 1685, 2024
BmeRI GACNNNNNGTC 1 cut(s) 2374
BmeT110I CYCGRG 3 cut(s) 722, 1157, 1563
BmgT120I GGNCC 9 cut(s) 27, 500, 572, 785, 1001, 1217, 1289, 1685, 2024
BmiI GGNNCC 4 cut(s) 502, 1003, 1024, 1075
BmrFI CCNGG 7 cut(s) 83, 164, 365, 617, 939, 1549, 1964
BmrI ACTGGG 4 cut(s) 831, 864, 2211, 2361
BmsI GCATC 3 cut(s) 2243, 2395, 2689
BmuI ACTGGG 4 cut(s) 831, 864, 2211, 2361
BpiI GAAGAC 1 cut(s) 2600
BpmI CTGGAG 3 cut(s) 279, 447, 1897
Bpu10I CCTNAGC 1 cut(s) 870
Bpu1102I GCTNAGC 1 cut(s) 893
BpuEI CTTGAG 5 cut(s) 485, 1265, 1418, 2070, 2360
BpuMI CCSGG 2 cut(s) 83, 365
Bsa29I ATCGAT 2 cut(s) 1520, 2268
BsaBI GATNNNNATC 1 cut(s) 245
BsaI GGTCTC 4 cut(s) 1305, 1394, 2007, 2569
BsaJI CCNNGG 8 cut(s) 81, 301, 723, 1548, 1611, 1962, 1963, 2403
BsaWI WCCGGW 2 cut(s) 49, 1860
BsaXI ACNNNNNCTCC 4 cut(s) 850, 880, 1985, 2015
Bsc4I CCNNNNNNNGG 7 cut(s) 24, 302, 803, 875, 1548, 1837, 2529
Bse118I RCCGGY 1 cut(s) 1860
Bse21I CCTNAGG 1 cut(s) 1316
Bse3DI GCAATG 1 cut(s) 1482
Bse8I GATNNNNATC 1 cut(s) 245
BseBI CCWGG 5 cut(s) 164, 617, 939, 1549, 1964
BseCI ATCGAT 2 cut(s) 1520, 2268
BseDI CCNNGG 8 cut(s) 81, 301, 723, 1548, 1611, 1962, 1963, 2403
BseGI GGATG 6 cut(s) 373, 504, 1005, 2143, 2170, 2211
BseJI GATNNNNATC 1 cut(s) 245
BseLI CCNNNNNNNGG 7 cut(s) 24, 302, 803, 875, 1548, 1837, 2529
BseMI GCAATG 1 cut(s) 1482
BseMII CTCAG 5 cut(s) 134, 861, 1330, 1746, 2517
BseRI GAGGAG 2 cut(s) 187, 1964
BseSI GKGCMC 1 cut(s) 186
BseX3I CGGCCG 1 cut(s) 177
BseXI GCAGC 3 cut(s) 390, 1555, 2093
BsgI GTGCAG 4 cut(s) 40, 1012, 1522, 2241
Bsh1236I CGCG 2 cut(s) 76, 90
Bsh1285I CGRYCG 1 cut(s) 180
BshFI GGCC 6 cut(s) 179, 254, 573, 787, 1218, 2533
BshTI ACCGGT 1 cut(s) 1860
BshVI ATCGAT 2 cut(s) 1520, 2268
BsiEI CGRYCG 1 cut(s) 180
BsiHKAI GWGCWC 2 cut(s) 876, 1251
BsiHKCI CYCGRG 3 cut(s) 722, 1157, 1563
BsiSI CCGG 4 cut(s) 50, 83, 365, 1861
BslFI GGGAC 5 cut(s) 63, 486, 871, 987, 1179
BslI CCNNNNNNNGG 7 cut(s) 24, 302, 803, 875, 1548, 1837, 2529
BsmAI GTCTC 8 cut(s) 423, 1305, 1394, 1883, 1962, 2007, 2423, 2569
BsmBI CGTCTC 1 cut(s) 1883
BsmFI GGGAC 5 cut(s) 63, 486, 871, 987, 1179
BsnI GGCC 6 cut(s) 179, 254, 573, 787, 1218, 2533
Bso31I GGTCTC 4 cut(s) 1305, 1394, 2007, 2569
BsoBI CYCGRG 3 cut(s) 722, 1157, 1563
Bsp1286I GDGCHC 3 cut(s) 186, 876, 1251
Bsp1407I TGTACA 2 cut(s) 599, 1806
Bsp143I GATC 9 cut(s) 576, 612, 636, 991, 1073, 1372, 1517, 1664, 2269
Bsp1720I GCTNAGC 1 cut(s) 893
Bsp19I CCATGG 1 cut(s) 1611
BspACI CCGC 5 cut(s) 282, 1493, 2122, 2408, 2630
BspANI GGCC 6 cut(s) 179, 254, 573, 787, 1218, 2533
BspCNI CTCAG 5 cut(s) 135, 862, 1329, 1747, 2518
BspDI ATCGAT 2 cut(s) 1520, 2268
BspFNI CGCG 2 cut(s) 76, 90
BspHI TCATGA 1 cut(s) 2107
BspLI GGNNCC 4 cut(s) 502, 1003, 1024, 1075
BspMAI CTGCAG 3 cut(s) 1104, 1303, 1573
BspMI ACCTGC 3 cut(s) 843, 1234, 2465
BspPI GGATC 7 cut(s) 571, 607, 1068, 1081, 1525, 1672, 2264
BspTI CTTAAG 1 cut(s) 455
BspTNI GGTCTC 4 cut(s) 1305, 1394, 2007, 2569
BsrDI GCAATG 1 cut(s) 1482
BsrFI RCCGGY 1 cut(s) 1860
BsrGI TGTACA 2 cut(s) 599, 1806
BssAI RCCGGY 1 cut(s) 1860
BssECI CCNNGG 8 cut(s) 81, 301, 723, 1548, 1611, 1962, 1963, 2403
BssMI GATC 9 cut(s) 576, 612, 636, 991, 1073, 1372, 1517, 1664, 2269
BssT1I CCWWGG 3 cut(s) 301, 1611, 2403
Bst2UI CCWGG 5 cut(s) 164, 617, 939, 1549, 1964
Bst4CI ACNGT 7 cut(s) 61, 599, 1388, 1464, 1804, 2422, 2434
Bst6I CTCTTC 3 cut(s) 19, 225, 1982
BstAFI CTTAAG 1 cut(s) 455
BstAUI TGTACA 2 cut(s) 599, 1806
BstC8I GCNNGC 3 cut(s) 88, 744, 1299
BstDEI CTNAG 6 cut(s) 143, 870, 893, 1316, 1755, 2526
BstDSI CCRYGG 1 cut(s) 1611
BstF5I GGATG 6 cut(s) 373, 504, 1005, 2143, 2170, 2211
BstFNI CGCG 2 cut(s) 76, 90
BstHHI GCGC 2 cut(s) 76, 92
BstKTI GATC 9 cut(s) 579, 615, 639, 994, 1076, 1375, 1520, 1667, 2272
BstMAI GTCTC 8 cut(s) 423, 1305, 1394, 1883, 1962, 2007, 2423, 2569
BstMBI GATC 9 cut(s) 576, 612, 636, 991, 1073, 1372, 1517, 1664, 2269
BstMCI CGRYCG 1 cut(s) 180
BstMWI GCNNNNNNNGC 7 cut(s) 460, 952, 2078, 2121, 2341, 2539, 2677
BstNI CCWGG 5 cut(s) 164, 617, 939, 1549, 1964
BstSCI CCNGG 7 cut(s) 81, 162, 363, 615, 937, 1547, 1962
BstSFI CTRYAG 3 cut(s) 1100, 1299, 1569
BstSLI GKGCMC 1 cut(s) 186
BstUI CGCG 2 cut(s) 76, 90
BstV1I GCAGC 3 cut(s) 390, 1555, 2093
BstV2I GAAGAC 1 cut(s) 2600
BstX2I RGATCY 3 cut(s) 612, 1073, 1664
BstXI CCANNNNNNTGG 1 cut(s) 1875
BstYI RGATCY 3 cut(s) 612, 1073, 1664
BstZI CGGCCG 1 cut(s) 177
Bsu15I ATCGAT 2 cut(s) 1520, 2268
Bsu36I CCTNAGG 1 cut(s) 1316
BsuI GTATCC 1 cut(s) 1875
BsuRI GGCC 6 cut(s) 179, 254, 573, 787, 1218, 2533
BsuTUI ATCGAT 2 cut(s) 1520, 2268
BtgI CCRYGG 1 cut(s) 1611
BtsCI GGATG 6 cut(s) 373, 504, 1005, 2143, 2170, 2211
BtsI GCAGTG 2 cut(s) 1029, 1510
BveI ACCTGC 3 cut(s) 843, 1234, 2465
Cac8I GCNNGC 3 cut(s) 88, 744, 1299
CaiI CAGNNNCTG 1 cut(s) 1509
CciI TCATGA 1 cut(s) 2107
CfoI GCGC 2 cut(s) 76, 92
Cfr10I RCCGGY 1 cut(s) 1860
Cfr13I GGNCC 9 cut(s) 27, 500, 572, 785, 1001, 1217, 1289, 1685, 2024
ClaI ATCGAT 2 cut(s) 1520, 2268
CseI GACGC 1 cut(s) 65
Csp6I GTAC 6 cut(s) 167, 600, 1223, 1439, 1807, 1817
CspAI ACCGGT 1 cut(s) 1860
CspCI CAANNNNNGTGG 2 cut(s) 1339, 1374
CviQI GTAC 6 cut(s) 167, 600, 1223, 1439, 1807, 1817
DdeI CTNAG 6 cut(s) 143, 870, 893, 1316, 1755, 2526
DpnI GATC 9 cut(s) 578, 614, 638, 993, 1075, 1374, 1519, 1666, 2271
DpnII GATC 9 cut(s) 576, 612, 636, 991, 1073, 1372, 1517, 1664, 2269
DrdI GACNNNNNNGTC 1 cut(s) 59
DriI GACNNNNNGTC 1 cut(s) 2374
DseDI GACNNNNNNGTC 1 cut(s) 59
EaeI YGGCCR 1 cut(s) 177
EagI CGGCCG 1 cut(s) 177
Eam1104I CTCTTC 3 cut(s) 19, 225, 1982
Eam1105I GACNNNNNGTC 1 cut(s) 2374
EarI CTCTTC 3 cut(s) 19, 225, 1982
EciI GGCGGA 1 cut(s) 2423
Ecl136II GAGCTC 1 cut(s) 874
EclXI CGGCCG 1 cut(s) 177
Eco130I CCWWGG 3 cut(s) 301, 1611, 2403
Eco147I AGGCCT 1 cut(s) 254
Eco24I GRGCYC 1 cut(s) 876
Eco31I GGTCTC 4 cut(s) 1305, 1394, 2007, 2569
Eco47I GGWCC 6 cut(s) 27, 500, 1001, 1289, 1685, 2024
Eco52I CGGCCG 1 cut(s) 177
Eco53kI GAGCTC 1 cut(s) 874
Eco57I CTGAAG 2 cut(s) 1123, 2463
Eco81I CCTNAGG 1 cut(s) 1316
Eco88I CYCGRG 3 cut(s) 722, 1157, 1563
EcoICRI GAGCTC 1 cut(s) 874
EcoO109I RGGNCCY 2 cut(s) 1001, 1685
EcoRI GAATTC 1 cut(s) 933
EcoRII CCWGG 5 cut(s) 162, 615, 937, 1547, 1962
EcoT14I CCWWGG 3 cut(s) 301, 1611, 2403
EcoT22I ATGCAT 2 cut(s) 2236, 2544
EcoT38I GRGCYC 1 cut(s) 876
ErhI CCWWGG 3 cut(s) 301, 1611, 2403
Esp3I CGTCTC 1 cut(s) 1883
FaqI GGGAC 5 cut(s) 63, 486, 871, 987, 1179
FauI CCCGC 3 cut(s) 275, 1500, 2129
FbaI TGATCA 1 cut(s) 1372
Fnu4HI GCNGC 3 cut(s) 379, 1569, 2082
FokI GGATG 6 cut(s) 380, 491, 992, 2150, 2177, 2198
FriOI GRGCYC 1 cut(s) 876
Fsp4HI GCNGC 3 cut(s) 379, 1569, 2082
FspBI CTAG 4 cut(s) 803, 1797, 1931, 2283
GlaI GCGC 2 cut(s) 75, 91
GluI GCNGC 3 cut(s) 379, 1569, 2082
GsuI CTGGAG 3 cut(s) 279, 447, 1897
HaeIII GGCC 6 cut(s) 179, 254, 573, 787, 1218, 2533
HapII CCGG 4 cut(s) 50, 83, 365, 1861
HgaI GACGC 1 cut(s) 65
HhaI GCGC 2 cut(s) 76, 92
Hin6I GCGC 2 cut(s) 74, 90
HinP1I GCGC 2 cut(s) 74, 90
HincII GTYRAC 3 cut(s) 852, 2437, 2635
HindII GTYRAC 3 cut(s) 852, 2437, 2635
HindIII AAGCTT 4 cut(s) 452, 461, 604, 890
HpaI GTTAAC 1 cut(s) 2635
HpaII CCGG 4 cut(s) 50, 83, 365, 1861
HphI GGTGA 6 cut(s) 110, 119, 953, 1461, 2446, 2735
Hpy166II GTNNAC 6 cut(s) 852, 1807, 1817, 2368, 2437, 2635
Hpy188I TCNGA 8 cut(s) 412, 636, 1119, 2191, 2311, 2349, 2394, 2698
Hpy8I GTNNAC 6 cut(s) 852, 1807, 1817, 2368, 2437, 2635
HpyAV CCTTC 5 cut(s) 40, 448, 992, 1147, 2353
HpyCH4III ACNGT 7 cut(s) 61, 599, 1388, 1464, 1804, 2422, 2434
HpyCH4IV ACGT 3 cut(s) 159, 1050, 2059
HpyF10VI GCNNNNNNNGC 7 cut(s) 460, 952, 2078, 2121, 2341, 2539, 2677
HpyF3I CTNAG 6 cut(s) 143, 870, 893, 1316, 1755, 2526
HpySE526I ACGT 3 cut(s) 159, 1050, 2059
HspAI GCGC 2 cut(s) 74, 90
Ksp22I TGATCA 1 cut(s) 1372
KspAI GTTAAC 1 cut(s) 2635
Kzo9I GATC 9 cut(s) 576, 612, 636, 991, 1073, 1372, 1517, 1664, 2269
LmnI GCTCC 1 cut(s) 2078
Lsp1109I GCAGC 3 cut(s) 390, 1555, 2093
LweI GCATC 3 cut(s) 2243, 2395, 2689
MaeI CTAG 4 cut(s) 803, 1797, 1931, 2283
MaeII ACGT 3 cut(s) 159, 1050, 2059
MaeIII GTNAC 6 cut(s) 61, 107, 842, 1505, 2261, 2452
MalI GATC 9 cut(s) 578, 614, 638, 993, 1075, 1374, 1519, 1666, 2271
MboI GATC 9 cut(s) 576, 612, 636, 991, 1073, 1372, 1517, 1664, 2269
MfeI CAATTG 1 cut(s) 2256
MflI RGATCY 3 cut(s) 612, 1073, 1664
MhlI GDGCHC 3 cut(s) 186, 876, 1251
MlyI GAGTC 3 cut(s) 1845, 2384, 2590
MmeI TCCRAC 2 cut(s) 1662, 2056
Mph1103I ATGCAT 2 cut(s) 2236, 2544
MroXI GAANNNNTTC 1 cut(s) 1043
MslI CAYNNNNRTG 4 cut(s) 1284, 1715, 2136, 2459
MspA1I CMGCKG 2 cut(s) 381, 1495
MspCI CTTAAG 1 cut(s) 455
MspI CCGG 4 cut(s) 50, 83, 365, 1861
MspR9I CCNGG 7 cut(s) 83, 164, 365, 617, 939, 1549, 1964
MunI CAATTG 1 cut(s) 2256
MvaI CCWGG 5 cut(s) 164, 617, 939, 1549, 1964
MvnI CGCG 2 cut(s) 76, 90
MwoI GCNNNNNNNGC 7 cut(s) 460, 952, 2078, 2121, 2341, 2539, 2677
NciI CCSGG 2 cut(s) 83, 365
NcoI CCATGG 1 cut(s) 1611
NdeII GATC 9 cut(s) 576, 612, 636, 991, 1073, 1372, 1517, 1664, 2269
NlaIV GGNNCC 4 cut(s) 502, 1003, 1024, 1075
NmeAIII GCCGAG 2 cut(s) 855, 2272
NmuCI GTSAC 4 cut(s) 61, 107, 1505, 2452
NsiI ATGCAT 2 cut(s) 2236, 2544
PaeR7I CTCGAG 1 cut(s) 1157
PagI TCATGA 1 cut(s) 2107
PaqCI CACCTGC 1 cut(s) 2465
PasI CCCWGGG 1 cut(s) 1963
PceI AGGCCT 1 cut(s) 254
PdmI GAANNNNTTC 1 cut(s) 1043
PflFI GACNNNGTC 1 cut(s) 1683
PflMI CCANNNNNTGG 1 cut(s) 302
PfoI TCCNGGA 2 cut(s) 363, 615
PinAI ACCGGT 1 cut(s) 1860
PkrI GCNGC 3 cut(s) 380, 1570, 2083
PleI GAGTC 3 cut(s) 1845, 2383, 2590
PpsI GAGTC 3 cut(s) 1845, 2383, 2590
PpuMI RGGWCCY 2 cut(s) 1001, 1685
PshBI ATTAAT 1 cut(s) 1152
Psp124BI GAGCTC 1 cut(s) 876
Psp1406I AACGTT 1 cut(s) 1050
Psp5II RGGWCCY 2 cut(s) 1001, 1685
Psp6I CCWGG 5 cut(s) 162, 615, 937, 1547, 1962
PspGI CCWGG 5 cut(s) 162, 615, 937, 1547, 1962
PspN4I GGNNCC 4 cut(s) 502, 1003, 1024, 1075
PspPI GGNCC 9 cut(s) 27, 500, 572, 785, 1001, 1217, 1289, 1685, 2024
PspPPI RGGWCCY 2 cut(s) 1001, 1685
PstI CTGCAG 3 cut(s) 1104, 1303, 1573
PstNI CAGNNNCTG 1 cut(s) 1509
PsuI RGATCY 3 cut(s) 612, 1073, 1664
PsyI GACNNNGTC 1 cut(s) 1683
PvuII CAGCTG 1 cut(s) 381
RsaI GTAC 6 cut(s) 168, 601, 1224, 1440, 1808, 1818
RsaNI GTAC 6 cut(s) 167, 600, 1223, 1439, 1807, 1817
RseI CAYNNNNRTG 4 cut(s) 1284, 1715, 2136, 2459
SacI GAGCTC 1 cut(s) 876
SatI GCNGC 3 cut(s) 379, 1569, 2082
Sau3AI GATC 9 cut(s) 576, 612, 636, 991, 1073, 1372, 1517, 1664, 2269
Sau96I GGNCC 9 cut(s) 27, 500, 572, 785, 1001, 1217, 1289, 1685, 2024
SchI GAGTC 3 cut(s) 1845, 2384, 2590
ScrFI CCNGG 7 cut(s) 83, 164, 365, 617, 939, 1549, 1964
SduI GDGCHC 3 cut(s) 186, 876, 1251
SfaNI GCATC 3 cut(s) 2243, 2395, 2689
SfcI CTRYAG 3 cut(s) 1100, 1299, 1569
Sfr274I CTCGAG 1 cut(s) 1157
SinI GGWCC 6 cut(s) 27, 500, 1001, 1289, 1685, 2024
SlaI CTCGAG 1 cut(s) 1157
SmiMI CAYNNNNRTG 4 cut(s) 1284, 1715, 2136, 2459
SmlI CTYRAG 7 cut(s) 455, 464, 1157, 1244, 1397, 2085, 2339
SmoI CTYRAG 7 cut(s) 455, 464, 1157, 1244, 1397, 2085, 2339
SseBI AGGCCT 1 cut(s) 254
SsiI CCGC 5 cut(s) 282, 1493, 2122, 2408, 2630
SspMI CTAG 4 cut(s) 803, 1797, 1931, 2283
SstI GAGCTC 1 cut(s) 876
StuI AGGCCT 1 cut(s) 254
StyD4I CCNGG 7 cut(s) 81, 162, 363, 615, 937, 1547, 1962
StyI CCWWGG 3 cut(s) 301, 1611, 2403
TaaI ACNGT 7 cut(s) 61, 599, 1388, 1464, 1804, 2422, 2434
TaiI ACGT 3 cut(s) 162, 1053, 2062
TaqI TCGA 8 cut(s) 134, 257, 1158, 1520, 2030, 2237, 2268, 2613
TaqII GACCGA 1 cut(s) 15
TatI WGTACW 3 cut(s) 599, 1806, 1816
TseFI GTSAC 4 cut(s) 61, 107, 1505, 2452
TseI GCWGC 3 cut(s) 378, 1568, 2081
Tsp45I GTSAC 4 cut(s) 61, 107, 1505, 2452
TspGWI ACGGA 2 cut(s) 1222, 1907
Tth111I GACNNNGTC 1 cut(s) 1683
Van91I CCANNNNNTGG 1 cut(s) 302
Vha464I CTTAAG 1 cut(s) 455
VpaK11BI GGWCC 6 cut(s) 27, 500, 1001, 1289, 1685, 2024
VspI ATTAAT 1 cut(s) 1152
XapI RAATTY 9 cut(s) 475, 525, 933, 1120, 1264, 1304, 1408, 1825, 2170
XcmI CCANNNNNNNNNTGG 2 cut(s) 274, 991
XhoI CTCGAG 1 cut(s) 1157
XmnI GAANNNNTTC 1 cut(s) 1043
XspI CTAG 4 cut(s) 803, 1797, 1931, 2283
Zsp2I ATGCAT 2 cut(s) 2236, 2544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.