Belongs to the protein kinase superfamily. Ser Thr protein kinase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 562 |
| AccB1I | GGYRCC | 2 cut(s) | 238, 1312 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 956 |
| AccBSI | CCGCTC | 1 cut(s) | 1288 |
| AccI | GTMKAC | 2 cut(s) | 764, 847 |
| AccIII | TCCGGA | 1 cut(s) | 516 |
| AciI | CCGC | 5 cut(s) | 317, 687, 1288, 1303, 1474 |
| AclWI | GGATC | 2 cut(s) | 553, 738 |
| AcoI | YGGCCR | 1 cut(s) | 1475 |
| AcsI | RAATTY | 2 cut(s) | 496, 1033 |
| AcuI | CTGAAG | 3 cut(s) | 570, 891, 1614 |
| AcvI | CACGTG | 1 cut(s) | 956 |
| AcyI | GRCGYC | 3 cut(s) | 239, 571, 1313 |
| AfaI | GTAC | 2 cut(s) | 1189, 1511 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 78, 343, 956, 1166, 1426 |
| AgsI | TTSAA | 5 cut(s) | 496, 504, 603, 780, 1164 |
| AjiI | CACGTC | 1 cut(s) | 398 |
| AjnI | CCWGG | 9 cut(s) | 319, 487, 541, 694, 1006, 1084, 1120, 1129, 1462 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1635, 1667 |
| Alw21I | GWGCWC | 2 cut(s) | 1093, 1208 |
| AlwI | GGATC | 2 cut(s) | 553, 738 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 443, 1529 |
| Ama87I | CYCGRG | 1 cut(s) | 624 |
| Aor13HI | TCCGGA | 1 cut(s) | 516 |
| AoxI | GGCC | 6 cut(s) | 69, 521, 1082, 1475, 1526, 1615 |
| ApeKI | GCWGC | 4 cut(s) | 58, 61, 446, 656 |
| ApoI | RAATTY | 2 cut(s) | 496, 1033 |
| AspLEI | GCGC | 3 cut(s) | 241, 962, 1315 |
| AspS9I | GGNCC | 5 cut(s) | 69, 522, 539, 1124, 1526 |
| AsuC2I | CCSGG | 1 cut(s) | 198 |
| AsuII | TTCGAA | 1 cut(s) | 914 |
| AsuNHI | GCTAGC | 1 cut(s) | 1586 |
| AvaI | CYCGRG | 1 cut(s) | 624 |
| AvaII | GGWCC | 2 cut(s) | 539, 1124 |
| BaeGI | GKGCMC | 1 cut(s) | 807 |
| BanI | GGYRCC | 2 cut(s) | 238, 1312 |
| BanII | GRGCYC | 4 cut(s) | 22, 1177, 1208, 1259 |
| BbrPI | CACGTG | 1 cut(s) | 956 |
| BbsI | GAAGAC | 1 cut(s) | 1141 |
| Bbv12I | GWGCWC | 2 cut(s) | 1093, 1208 |
| BbvCI | CCTCAGC | 1 cut(s) | 928 |
| BbvI | GCAGC | 4 cut(s) | 70, 73, 433, 668 |
| BccI | CCATC | 3 cut(s) | 868, 958, 1012 |
| BceAI | ACGGC | 2 cut(s) | 783, 1462 |
| BciT130I | CCWGG | 9 cut(s) | 321, 489, 543, 696, 1008, 1086, 1122, 1131, 1464 |
| BciVI | GTATCC | 1 cut(s) | 524 |
| BcnI | CCSGG | 1 cut(s) | 198 |
| BfaI | CTAG | 9 cut(s) | 113, 417, 422, 450, 873, 981, 1074, 1101, 1587 |
| BfmI | CTRYAG | 1 cut(s) | 654 |
| BfoI | RGCGCY | 2 cut(s) | 242, 1316 |
| BfuAI | ACCTGC | 1 cut(s) | 562 |
| BfuI | GTATCC | 1 cut(s) | 524 |
| BglII | AGATCT | 2 cut(s) | 773, 1103 |
| BisI | GCNGC | 5 cut(s) | 59, 62, 447, 657, 1475 |
| BlsI | GCNGC | 5 cut(s) | 60, 63, 448, 658, 1476 |
| BmcAI | AGTACT | 1 cut(s) | 1189 |
| Bme18I | GGWCC | 2 cut(s) | 539, 1124 |
| BmeT110I | CYCGRG | 1 cut(s) | 624 |
| BmgBI | CACGTC | 1 cut(s) | 398 |
| BmgT120I | GGNCC | 5 cut(s) | 69, 522, 539, 1124, 1526 |
| BmiI | GGNNCC | 6 cut(s) | 240, 385, 540, 1176, 1314, 1352 |
| BmsI | GCATC | 4 cut(s) | 839, 971, 1012, 1508 |
| BmtI | GCTAGC | 1 cut(s) | 1590 |
| BpiI | GAAGAC | 1 cut(s) | 1141 |
| BpmI | CTGGAG | 1 cut(s) | 276 |
| Bpu10I | CCTNAGC | 3 cut(s) | 21, 65, 928 |
| Bpu14I | TTCGAA | 1 cut(s) | 914 |
| BpuEI | CTTGAG | 1 cut(s) | 724 |
| BpuMI | CCSGG | 1 cut(s) | 198 |
| BsaAI | YACGTR | 1 cut(s) | 956 |
| BsaHI | GRCGYC | 3 cut(s) | 239, 571, 1313 |
| BsaJI | CCNNGG | 7 cut(s) | 320, 487, 488, 645, 694, 986, 996 |
| BsaWI | WCCGGW | 1 cut(s) | 516 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 574, 604 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 78, 343, 956, 1166, 1426 |
| Bse118I | RCCGGY | 1 cut(s) | 235 |
| Bse1I | ACTGG | 7 cut(s) | 259, 571, 793, 920, 944, 1314, 1683 |
| BseAI | TCCGGA | 1 cut(s) | 516 |
| BseBI | CCWGG | 9 cut(s) | 321, 489, 543, 696, 1008, 1086, 1122, 1131, 1464 |
| BseDI | CCNNGG | 7 cut(s) | 320, 487, 488, 645, 694, 986, 996 |
| BseGI | GGATG | 4 cut(s) | 860, 1027, 1373, 1499 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 78, 343, 956, 1166, 1426 |
| BseMII | CTCAG | 4 cut(s) | 35, 79, 681, 942 |
| BseNI | ACTGG | 7 cut(s) | 259, 571, 793, 920, 944, 1314, 1683 |
| BseRI | GAGGAG | 3 cut(s) | 318, 1332, 1554 |
| BseSI | GKGCMC | 1 cut(s) | 807 |
| BseX3I | CGGCCG | 1 cut(s) | 1475 |
| BseXI | GCAGC | 4 cut(s) | 70, 73, 433, 668 |
| BseYI | CCCAGC | 2 cut(s) | 432, 1094 |
| BsgI | GTGCAG | 3 cut(s) | 228, 390, 1515 |
| Bsh1285I | CGRYCG | 2 cut(s) | 851, 1478 |
| BshFI | GGCC | 6 cut(s) | 71, 523, 1084, 1477, 1528, 1617 |
| BshNI | GGYRCC | 2 cut(s) | 238, 1312 |
| BsiEI | CGRYCG | 2 cut(s) | 851, 1478 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1093, 1208 |
| BsiHKCI | CYCGRG | 1 cut(s) | 624 |
| BsiSI | CCGG | 3 cut(s) | 198, 236, 517 |
| BslFI | GGGAC | 1 cut(s) | 337 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 78, 343, 956, 1166, 1426 |
| BsmFI | GGGAC | 1 cut(s) | 337 |
| BsmI | GAATGC | 3 cut(s) | 1217, 1231, 1458 |
| BsnI | GGCC | 6 cut(s) | 71, 523, 1084, 1477, 1528, 1617 |
| BsoBI | CYCGRG | 1 cut(s) | 624 |
| Bsp119I | TTCGAA | 1 cut(s) | 914 |
| Bsp1286I | GDGCHC | 6 cut(s) | 22, 807, 1093, 1177, 1208, 1259 |
| Bsp13I | TCCGGA | 1 cut(s) | 516 |
| Bsp1407I | TGTACA | 1 cut(s) | 1509 |
| Bsp143I | GATC | 9 cut(s) | 145, 545, 605, 743, 773, 1103, 1400, 1520, 1673 |
| Bsp19I | CCATGG | 1 cut(s) | 986 |
| BspACI | CCGC | 5 cut(s) | 317, 687, 1288, 1303, 1474 |
| BspANI | GGCC | 6 cut(s) | 71, 523, 1084, 1477, 1528, 1617 |
| BspCNI | CTCAG | 4 cut(s) | 34, 78, 680, 941 |
| BspEI | TCCGGA | 1 cut(s) | 516 |
| BspLI | GGNNCC | 6 cut(s) | 240, 385, 540, 1176, 1314, 1352 |
| BspMAI | CTGCAG | 1 cut(s) | 658 |
| BspMI | ACCTGC | 1 cut(s) | 562 |
| BspOI | GCTAGC | 1 cut(s) | 1590 |
| BspPI | GGATC | 2 cut(s) | 553, 738 |
| BspQI | GCTCTTC | 1 cut(s) | 90 |
| BspT104I | TTCGAA | 1 cut(s) | 914 |
| BspT107I | GGYRCC | 2 cut(s) | 238, 1312 |
| BsrBI | CCGCTC | 1 cut(s) | 1288 |
| BsrFI | RCCGGY | 1 cut(s) | 235 |
| BsrGI | TGTACA | 1 cut(s) | 1509 |
| BsrI | ACTGG | 7 cut(s) | 259, 571, 793, 920, 944, 1314, 1683 |
| BssAI | RCCGGY | 1 cut(s) | 235 |
| BssECI | CCNNGG | 7 cut(s) | 320, 487, 488, 645, 694, 986, 996 |
| BssMI | GATC | 9 cut(s) | 145, 545, 605, 743, 773, 1103, 1400, 1520, 1673 |
| BssNI | GRCGYC | 3 cut(s) | 239, 571, 1313 |
| BssT1I | CCWWGG | 1 cut(s) | 986 |
| Bst2UI | CCWGG | 9 cut(s) | 321, 489, 543, 696, 1008, 1086, 1122, 1131, 1464 |
| Bst4CI | ACNGT | 9 cut(s) | 580, 649, 756, 802, 852, 973, 1385, 1514, 1546 |
| Bst6I | CTCTTC | 3 cut(s) | 90, 119, 162 |
| BstACI | GRCGYC | 3 cut(s) | 239, 571, 1313 |
| BstAUI | TGTACA | 1 cut(s) | 1509 |
| BstBAI | YACGTR | 1 cut(s) | 956 |
| BstBI | TTCGAA | 1 cut(s) | 914 |
| BstC8I | GCNNGC | 8 cut(s) | 39, 102, 237, 243, 247, 444, 1530, 1588 |
| BstDEI | CTNAG | 5 cut(s) | 21, 65, 460, 667, 928 |
| BstDSI | CCRYGG | 2 cut(s) | 645, 986 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 76, 1164 |
| BstF5I | GGATG | 4 cut(s) | 860, 1027, 1373, 1499 |
| BstH2I | RGCGCY | 2 cut(s) | 242, 1316 |
| BstHHI | GCGC | 3 cut(s) | 241, 962, 1315 |
| BstKTI | GATC | 9 cut(s) | 148, 548, 608, 746, 776, 1106, 1403, 1523, 1676 |
| BstMBI | GATC | 9 cut(s) | 145, 545, 605, 743, 773, 1103, 1400, 1520, 1673 |
| BstMCI | CGRYCG | 2 cut(s) | 851, 1478 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 58, 1321 |
| BstNI | CCWGG | 9 cut(s) | 321, 489, 543, 696, 1008, 1086, 1122, 1131, 1464 |
| BstSFI | CTRYAG | 1 cut(s) | 654 |
| BstSLI | GKGCMC | 1 cut(s) | 807 |
| BstV1I | GCAGC | 4 cut(s) | 70, 73, 433, 668 |
| BstV2I | GAAGAC | 1 cut(s) | 1141 |
| BstX2I | RGATCY | 3 cut(s) | 743, 773, 1103 |
| BstYI | RGATCY | 3 cut(s) | 743, 773, 1103 |
| BstZI | CGGCCG | 1 cut(s) | 1475 |
| BsuI | GTATCC | 1 cut(s) | 524 |
| BsuRI | GGCC | 6 cut(s) | 71, 523, 1084, 1477, 1528, 1617 |
| BtgI | CCRYGG | 2 cut(s) | 645, 986 |
| BtgZI | GCGATG | 2 cut(s) | 56, 948 |
| BtrI | CACGTC | 1 cut(s) | 398 |
| BtsCI | GGATG | 4 cut(s) | 860, 1027, 1373, 1499 |
| BtsI | GCAGTG | 1 cut(s) | 1663 |
| BtsIMutI | CAGTG | 7 cut(s) | 585, 951, 978, 1519, 1606, 1663, 1690 |
| BveI | ACCTGC | 1 cut(s) | 562 |
| Cac8I | GCNNGC | 8 cut(s) | 39, 102, 237, 243, 247, 444, 1530, 1588 |
| CaiI | CAGNNNCTG | 2 cut(s) | 443, 1529 |
| CfoI | GCGC | 3 cut(s) | 241, 962, 1315 |
| Cfr10I | RCCGGY | 1 cut(s) | 235 |
| Cfr13I | GGNCC | 5 cut(s) | 69, 522, 539, 1124, 1526 |
| CseI | GACGC | 1 cut(s) | 579 |
| Csp6I | GTAC | 2 cut(s) | 1188, 1510 |
| CviAII | CATG | 7 cut(s) | 367, 856, 987, 1152, 1346, 1441, 1691 |
| CviQI | GTAC | 2 cut(s) | 1188, 1510 |
| DdeI | CTNAG | 5 cut(s) | 21, 65, 460, 667, 928 |
| DinI | GGCGCC | 2 cut(s) | 240, 1314 |
| DpnI | GATC | 9 cut(s) | 147, 547, 607, 745, 775, 1105, 1402, 1522, 1675 |
| DpnII | GATC | 9 cut(s) | 145, 545, 605, 743, 773, 1103, 1400, 1520, 1673 |
| EaeI | YGGCCR | 1 cut(s) | 1475 |
| EagI | CGGCCG | 1 cut(s) | 1475 |
| Eam1104I | CTCTTC | 3 cut(s) | 90, 119, 162 |
| EarI | CTCTTC | 3 cut(s) | 90, 119, 162 |
| EciI | GGCGGA | 1 cut(s) | 306 |
| Ecl136II | GAGCTC | 1 cut(s) | 1206 |
| EclXI | CGGCCG | 1 cut(s) | 1475 |
| Eco130I | CCWWGG | 1 cut(s) | 986 |
| Eco24I | GRGCYC | 4 cut(s) | 22, 1177, 1208, 1259 |
| Eco47I | GGWCC | 2 cut(s) | 539, 1124 |
| Eco52I | CGGCCG | 1 cut(s) | 1475 |
| Eco53kI | GAGCTC | 1 cut(s) | 1206 |
| Eco57I | CTGAAG | 3 cut(s) | 570, 891, 1614 |
| Eco72I | CACGTG | 1 cut(s) | 956 |
| Eco88I | CYCGRG | 1 cut(s) | 624 |
| EcoICRI | GAGCTC | 1 cut(s) | 1206 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 76, 1164 |
| EcoO109I | RGGNCCY | 3 cut(s) | 69, 522, 1526 |
| EcoRI | GAATTC | 1 cut(s) | 496 |
| EcoRII | CCWGG | 9 cut(s) | 319, 487, 541, 694, 1006, 1084, 1120, 1129, 1462 |
| EcoT14I | CCWWGG | 1 cut(s) | 986 |
| EcoT38I | GRGCYC | 4 cut(s) | 22, 1177, 1208, 1259 |
| EgeI | GGCGCC | 2 cut(s) | 240, 1314 |
| EheI | GGCGCC | 2 cut(s) | 240, 1314 |
| ErhI | CCWWGG | 1 cut(s) | 986 |
| FaeI | CATG | 7 cut(s) | 370, 859, 990, 1155, 1349, 1444, 1694 |
| FaqI | GGGAC | 1 cut(s) | 337 |
| FatI | CATG | 7 cut(s) | 366, 855, 986, 1151, 1345, 1440, 1690 |
| FauI | CCCGC | 1 cut(s) | 1281 |
| FblI | GTMKAC | 2 cut(s) | 764, 847 |
| Fnu4HI | GCNGC | 5 cut(s) | 59, 62, 447, 657, 1475 |
| FokI | GGATG | 4 cut(s) | 847, 1034, 1360, 1486 |
| FriOI | GRGCYC | 4 cut(s) | 22, 1177, 1208, 1259 |
| Fsp4HI | GCNGC | 5 cut(s) | 59, 62, 447, 657, 1475 |
| FspBI | CTAG | 9 cut(s) | 113, 417, 422, 450, 873, 981, 1074, 1101, 1587 |
| GlaI | GCGC | 3 cut(s) | 240, 961, 1314 |
| GluI | GCNGC | 5 cut(s) | 59, 62, 447, 657, 1475 |
| GsaI | CCCAGC | 2 cut(s) | 436, 1098 |
| GsuI | CTGGAG | 1 cut(s) | 276 |
| HaeII | RGCGCY | 2 cut(s) | 242, 1316 |
| HaeIII | GGCC | 6 cut(s) | 71, 523, 1084, 1477, 1528, 1617 |
| HapII | CCGG | 3 cut(s) | 198, 236, 517 |
| HgaI | GACGC | 1 cut(s) | 579 |
| HhaI | GCGC | 3 cut(s) | 241, 962, 1315 |
| Hin1I | GRCGYC | 3 cut(s) | 239, 571, 1313 |
| Hin1II | CATG | 7 cut(s) | 370, 859, 990, 1155, 1349, 1444, 1694 |
| Hin6I | GCGC | 3 cut(s) | 239, 960, 1313 |
| HinP1I | GCGC | 3 cut(s) | 239, 960, 1313 |
| HincII | GTYRAC | 2 cut(s) | 612, 848 |
| HindII | GTYRAC | 2 cut(s) | 612, 848 |
| HinfI | GANTC | 6 cut(s) | 4, 26, 325, 353, 373, 1559 |
| HpaII | CCGG | 3 cut(s) | 198, 236, 517 |
| Hpy166II | GTNNAC | 5 cut(s) | 612, 645, 765, 848, 1606 |
| Hpy188I | TCNGA | 7 cut(s) | 9, 166, 280, 402, 1000, 1558, 1594 |
| Hpy8I | GTNNAC | 5 cut(s) | 612, 645, 765, 848, 1606 |
| Hpy99I | CGWCG | 1 cut(s) | 849 |
| HpyAV | CCTTC | 9 cut(s) | 76, 82, 127, 348, 609, 915, 1137, 1164, 1170 |
| HpyCH4III | ACNGT | 9 cut(s) | 580, 649, 756, 802, 852, 973, 1385, 1514, 1546 |
| HpyCH4IV | ACGT | 3 cut(s) | 397, 955, 1435 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 58, 1321 |
| HpyF3I | CTNAG | 5 cut(s) | 21, 65, 460, 667, 928 |
| HpySE526I | ACGT | 3 cut(s) | 397, 955, 1435 |
| Hsp92I | GRCGYC | 3 cut(s) | 239, 571, 1313 |
| Hsp92II | CATG | 7 cut(s) | 370, 859, 990, 1155, 1349, 1444, 1694 |
| HspAI | GCGC | 3 cut(s) | 239, 960, 1313 |
| KasI | GGCGCC | 2 cut(s) | 238, 1312 |
| Kpn2I | TCCGGA | 1 cut(s) | 516 |
| KroI | GCCGGC | 1 cut(s) | 235 |
| KroNI | GCCGGC | 1 cut(s) | 237 |
| Kzo9I | GATC | 9 cut(s) | 145, 545, 605, 743, 773, 1103, 1400, 1520, 1673 |
| LguI | GCTCTTC | 1 cut(s) | 90 |
| LmnI | GCTCC | 7 cut(s) | 257, 453, 595, 889, 1088, 1180, 1203 |
| Lsp1109I | GCAGC | 4 cut(s) | 70, 73, 433, 668 |
| LweI | GCATC | 4 cut(s) | 839, 971, 1012, 1508 |
| MaeI | CTAG | 9 cut(s) | 113, 417, 422, 450, 873, 981, 1074, 1101, 1587 |
| MaeII | ACGT | 3 cut(s) | 397, 955, 1435 |
| MaeIII | GTNAC | 2 cut(s) | 580, 1436 |
| MalI | GATC | 9 cut(s) | 147, 547, 607, 745, 775, 1105, 1402, 1522, 1675 |
| MbiI | CCGCTC | 1 cut(s) | 1288 |
| MboI | GATC | 9 cut(s) | 145, 545, 605, 743, 773, 1103, 1400, 1520, 1673 |
| MflI | RGATCY | 3 cut(s) | 743, 773, 1103 |
| MhlI | GDGCHC | 6 cut(s) | 22, 807, 1093, 1177, 1208, 1259 |
| MluCI | AATT | 7 cut(s) | 274, 496, 678, 711, 732, 1033, 1468 |
| Mly113I | GGCGCC | 2 cut(s) | 239, 1313 |
| MlyI | GAGTC | 3 cut(s) | 35, 319, 382 |
| MmeI | TCCRAC | 1 cut(s) | 425 |
| MreI | CGCCGGCG | 1 cut(s) | 235 |
| MroI | TCCGGA | 1 cut(s) | 516 |
| MroNI | GCCGGC | 1 cut(s) | 235 |
| MseI | TTAA | 7 cut(s) | 216, 311, 635, 681, 1037, 1242, 1265 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1197 |
| MspI | CCGG | 3 cut(s) | 198, 236, 517 |
| Mva1269I | GAATGC | 3 cut(s) | 1217, 1231, 1458 |
| MvaI | CCWGG | 9 cut(s) | 321, 489, 543, 696, 1008, 1086, 1122, 1131, 1464 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 58, 1321 |
| NaeI | GCCGGC | 1 cut(s) | 237 |
| NarI | GGCGCC | 2 cut(s) | 239, 1313 |
| NciI | CCSGG | 1 cut(s) | 198 |
| NcoI | CCATGG | 1 cut(s) | 986 |
| NdeII | GATC | 9 cut(s) | 145, 545, 605, 743, 773, 1103, 1400, 1520, 1673 |
| NgoMIV | GCCGGC | 1 cut(s) | 235 |
| NheI | GCTAGC | 1 cut(s) | 1586 |
| NlaIII | CATG | 7 cut(s) | 370, 859, 990, 1155, 1349, 1444, 1694 |
| NlaIV | GGNNCC | 6 cut(s) | 240, 385, 540, 1176, 1314, 1352 |
| NmuCI | GTSAC | 2 cut(s) | 580, 1436 |
| NspV | TTCGAA | 1 cut(s) | 914 |
| PaeR7I | CTCGAG | 1 cut(s) | 624 |
| PasI | CCCWGGG | 1 cut(s) | 488 |
| PciSI | GCTCTTC | 1 cut(s) | 90 |
| PctI | GAATGC | 3 cut(s) | 1217, 1231, 1458 |
| PdiI | GCCGGC | 1 cut(s) | 237 |
| PfeI | GAWTC | 3 cut(s) | 4, 353, 1559 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 956 |
| PfoI | TCCNGGA | 2 cut(s) | 541, 1129 |
| PkrI | GCNGC | 5 cut(s) | 60, 63, 448, 658, 1476 |
| PleI | GAGTC | 3 cut(s) | 34, 319, 381 |
| PluTI | GGCGCC | 2 cut(s) | 242, 1316 |
| PmaCI | CACGTG | 1 cut(s) | 956 |
| PmlI | CACGTG | 1 cut(s) | 956 |
| PpsI | GAGTC | 3 cut(s) | 34, 319, 381 |
| Ppu21I | YACGTR | 1 cut(s) | 956 |
| Psp124BI | GAGCTC | 1 cut(s) | 1208 |
| Psp6I | CCWGG | 9 cut(s) | 319, 487, 541, 694, 1006, 1084, 1120, 1129, 1462 |
| PspCI | CACGTG | 1 cut(s) | 956 |
| PspFI | CCCAGC | 2 cut(s) | 432, 1094 |
| PspGI | CCWGG | 9 cut(s) | 319, 487, 541, 694, 1006, 1084, 1120, 1129, 1462 |
| PspN4I | GGNNCC | 6 cut(s) | 240, 385, 540, 1176, 1314, 1352 |
| PspPI | GGNCC | 5 cut(s) | 69, 522, 539, 1124, 1526 |
| PstI | CTGCAG | 1 cut(s) | 658 |
| PstNI | CAGNNNCTG | 2 cut(s) | 443, 1529 |
| PsuI | RGATCY | 3 cut(s) | 743, 773, 1103 |
| RsaI | GTAC | 2 cut(s) | 1189, 1511 |
| RsaNI | GTAC | 2 cut(s) | 1188, 1510 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1197 |
| SacI | GAGCTC | 1 cut(s) | 1208 |
| SalI | GTCGAC | 1 cut(s) | 846 |
| SapI | GCTCTTC | 1 cut(s) | 90 |
| SaqAI | TTAA | 7 cut(s) | 216, 311, 635, 681, 1037, 1242, 1265 |
| SatI | GCNGC | 5 cut(s) | 59, 62, 447, 657, 1475 |
| Sau3AI | GATC | 9 cut(s) | 145, 545, 605, 743, 773, 1103, 1400, 1520, 1673 |
| Sau96I | GGNCC | 5 cut(s) | 69, 522, 539, 1124, 1526 |
| ScaI | AGTACT | 1 cut(s) | 1189 |
| SchI | GAGTC | 3 cut(s) | 35, 319, 382 |
| SduI | GDGCHC | 6 cut(s) | 22, 807, 1093, 1177, 1208, 1259 |
| SfaNI | GCATC | 4 cut(s) | 839, 971, 1012, 1508 |
| SfcI | CTRYAG | 1 cut(s) | 654 |
| SfoI | GGCGCC | 2 cut(s) | 240, 1314 |
| Sfr274I | CTCGAG | 1 cut(s) | 624 |
| SfuI | TTCGAA | 1 cut(s) | 914 |
| SgrAI | CRCCGGYG | 1 cut(s) | 235 |
| SinI | GGWCC | 2 cut(s) | 539, 1124 |
| SlaI | CTCGAG | 1 cut(s) | 624 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1197 |
| SmlI | CTYRAG | 2 cut(s) | 624, 739 |
| SmoI | CTYRAG | 2 cut(s) | 624, 739 |
| Sse9I | AATT | 7 cut(s) | 274, 496, 678, 711, 732, 1033, 1468 |
| SsiI | CCGC | 5 cut(s) | 317, 687, 1288, 1303, 1474 |
| SspDI | GGCGCC | 2 cut(s) | 238, 1312 |
| SspI | AATATT | 1 cut(s) | 1492 |
| SspMI | CTAG | 9 cut(s) | 113, 417, 422, 450, 873, 981, 1074, 1101, 1587 |
| SstI | GAGCTC | 1 cut(s) | 1208 |
| StyI | CCWWGG | 1 cut(s) | 986 |
| TaaI | ACNGT | 9 cut(s) | 580, 649, 756, 802, 852, 973, 1385, 1514, 1546 |
| TaiI | ACGT | 3 cut(s) | 400, 958, 1438 |
| TaqI | TCGA | 7 cut(s) | 174, 625, 674, 847, 914, 1016, 1672 |
| TasI | AATT | 7 cut(s) | 274, 496, 678, 711, 732, 1033, 1468 |
| TatI | WGTACW | 2 cut(s) | 1187, 1509 |
| TauI | GCSGC | 1 cut(s) | 1477 |
| TfiI | GAWTC | 3 cut(s) | 4, 353, 1559 |
| Tru1I | TTAA | 7 cut(s) | 216, 311, 635, 681, 1037, 1242, 1265 |
| Tru9I | TTAA | 7 cut(s) | 216, 311, 635, 681, 1037, 1242, 1265 |
| TscAI | CASTG | 7 cut(s) | 585, 951, 978, 1519, 1606, 1663, 1690 |
| TseFI | GTSAC | 2 cut(s) | 580, 1436 |
| TseI | GCWGC | 4 cut(s) | 58, 61, 446, 656 |
| Tsp45I | GTSAC | 2 cut(s) | 580, 1436 |
| TspDTI | ATGAA | 4 cut(s) | 17, 62, 134, 330 |
| TspRI | CASTG | 7 cut(s) | 585, 951, 978, 1519, 1606, 1663, 1690 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 956 |
| VpaK11BI | GGWCC | 2 cut(s) | 539, 1124 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 76, 1164 |
| XapI | RAATTY | 2 cut(s) | 496, 1033 |
| XbaI | TCTAGA | 5 cut(s) | 112, 416, 980, 1073, 1100 |
| XhoI | CTCGAG | 1 cut(s) | 624 |
| XmiI | GTMKAC | 2 cut(s) | 764, 847 |
| XspI | CTAG | 9 cut(s) | 113, 417, 422, 450, 873, 981, 1074, 1101, 1587 |
| ZrmI | AGTACT | 1 cut(s) | 1189 |