Rroxscaffold_4G00282890
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000383
Physical Location & Seq
Forward (+)
4992543 .. 4999361
6819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00282890.1

Sequence Viewer

Length: 2961 bp
ATGGAGGAGACGGTGGTTTTGAAATGGAGGAAGAACAAGGTTGTGGTTCTGTGGTTTGGGTTGTCTCTGTTTCTGCTTTTTCCTCTTGCTACGTCACTGAACGATGAAGGGAAAGCCTTGATGGCAATCAAGGCCTCGTTCAGCAATGTAGCCAATGTTTTACTGGACTGGAATGATGCTCATGACGAAGATTTCTGTTCTTGGCGTGGAGTATTCTGTGACAATGTCAGCCTCTCTGTGGCCTCCTTGAATTTGTCCAGCTTGAATCTGGGCGGGGAGATTTCGCCAGCCCTTGGAGATTTGGGGAACTTGGAATCCATAGACTTGCAGGGGAATAAGTTAACTGGTCAAATCCCGGATGAGATTGGCAACTGTGCTTCTCTTATATATCTGGATTTGTCTGACAATAATCTGTCTGGAGACATACCCTTCTCAGTTTCAAAGCTTAAGAAGCTTGAGGTTTTTAATTTGAAGAACAATCAGTTGACTGGTCCTATCCCTACAACTCTTACCCAAATTCCAAACCTTAAAACTCTTGATCTTGCTCGGAACCAGCTCACTGGTGAGATACCAAGGCTAATATATTGGAATGAAGTTTTGCAGTACCTTGGATTGCGAGGCAACTCTTTGACTGGAGCGCTGTCCCCTGATATGTGTCAATTAACTGGTCTGTGGTACTTTGATGTTAGAGGCAATAACCTGACTGGCCTGATCCCCGACAACATTGGCAACTGTACAAGCTTTGAGATCCTGGACATATCATACAATCAGATCACTGGTGGGATTCCATATAACATTGGATTTTTACAAGTGGCTACCCTGTCACTACAAGGGAATAGACTGACTGGAAAGATTCCTGAGGTTATTGGTTTAATGCAGGCTCTTGCTGTTTTGGATTTAAGTGAGAATGAACTTGTAGGGCCAATTCCACCGATACTAGGCAATTTATCATACACTGGGAAACTATACCTGCATGGTAACAAGTTGACTGGGACAATTCCTCCTGAGCTTGGCAATATGTCAAAGCTTAGCTATTTGCAATTAAATGACAACAATCTGGTTGGTAGAATTCCTGGTGAGCTTGGGAAGCTGGACCAGTTGTTTGAATTGAATCTTGCCAACAATGATCTGGAAGGTCCCATCCCACATGACATTGGTTCCTGCACTGCTTTGAATCAATTCAATGTTCATGGAAATCGCTTGACAGGATCCATTCCTTTAGCATTGAGAAATCTGCAGAGCTTGACCTATCTGAATTTGTCAGCAAATGCCTTCAGTGGTAGAATTAATCTTGAGTTGGGACATATCATCAATCTTGACACTTTGGATTTGTCCAGCAACAATTTCTCTGGCCCTGTTCCTGCTTCTGTTGGCAATCTTGAGCACCTCCTGACTTTGAATTTGAGCGGGAATCGTCTTGATGGTCCTTTGCCTGCAGAATTTGGGGACCTCAGGAGTGTTCAAATTATTGATATGTCATTCAACAATCTCTCTGGGAGCATTCCCTCAGAATTGGGTCAATTGCAGAACCTTGCGGCATTGTTGCTTAATAACAACAACTTGCGTGGAAAGATACCTGATCAGCTAACCAACTGTTTCAGCCTTGAGACCTTGAATTTTTCATACAACAATCTGTCAGGCATTGTACCTCCCATGAGGAATTTTTCACGTTTTTCACCAAACAGCTTCATTGCAAACCCGCTGTTGTGCAGTGACTGGTTGGGGTCGATATGCAGACCCGTTGCCCCCAAGTCCAGGGCTGTATTCTCCCGAGCAGCAGTTGTCTGTATGGCGTTAGGCTTCATCACATTATTATCCATGGTAATAGTTGCGATTTACAAGTCCAACCAACCAAAGCAATTAATGATGGGATCTACCAAGAATGGACAAGGTCCTGCTAAACTTGTAATCCTTCACATGGATATGGCTATACACAGCTTTGATGATATAATGAGAATCACTGAGAATCTAAATGAGAAGTATATCATAGGGTATGGTGCTTCTAGCACAGTGTACAAGTGTGTACTGAAAAATTCCCGACCAATGGCAATCAAGCGACTCTACAACCGGTATCCACACAACTGGAGGGAGTTTGAGACGGAACTTGAAACCATTGGCAGTATCAAGCATAGAAATTTAGTCAGTTTGCATGGGTATTCACTGTCTCCCCTGGGCAATCTCCTCTTCTATGACTACATGGAGAACGGGTCTCTGTGGGATATGCTTCATGGACCATCGAAAAAGGTTAAACTTGATTGGGAAACACGTTTGAAAATAGCAGTTGGAGCAGCTCAAGGACTTGCTTATCTTCACCATGACTGCAACCCCCGCATTATTCACAGGGATGTGAAATCCTCTAATATCCTGCTGGATGAGAATTTTGAAGCTCATCTCTCTGATTTTGGAATTGCAAAATCCATCCCCAGTGCAGATACCCATGCATCGACTTATGTTCTGGGAACAATTGGTTACATCGATCCAGAATATGCTAGGACTTCTCGGCTCAATGAAAAATCTGATGTATATAGCTTTGGCATTGTGCTTCTTGAGCTACTGACTGGGAAGAAGGCTGTGGACAAGGAGTCCAACTTGCATCATCTGATATTGTCCAAGGCGGATAACAATACTGTAATGGAGACTGTTGACCCTGAAGTTTCTGTCACCTGCATGGATATAACTCATGTCAAGAAAACTTTTCAACTCGCCCTGCTGTGCACCAAGCGAAACCCCACTGAGAGGCCAACAATGCATGAAGTTGCAAGGGTTTTGGTCTCCCTCCTCCCTCTACATCACTCAAAGAAGCCTCTTTCAAAGGGTATCGACTATGATAAGTTTGCGGTTAACAAGGAACATCAACAACCGAAAGTGCAACAGCAGAAGCCTATTCGGCAAGAAAGTCATTCATCTGATGCTCAATGGTTTCTTAGGTTTGGTGAGGTTATATCCAAGAACACCCTCTAA

Protein Analysis

986

Amino Acids

108.96

Weight (kDa)

6.28

Isoelectric Point (pI)

26.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 33 - 74 4.3e-11 Leucine rich repeat N-terminal domain
LRR_4 PF12799 127 - 163 5.5e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 128 - 186 3.9e-08 Leucine rich repeat
LRR_8 PF13855 321 - 377 2.7e-06 Leucine rich repeat
LRR_14 PF23598 336 - 446 5.1e-08 Leucine-rich repeat region
LRR_8 PF13855 438 - 497 1.6e-08 Leucine rich repeat
LRR_14 PF23598 455 - 540 4e-08 Leucine-rich repeat region
Pkinase PF00069 659 - 920 9.6e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 662 - 922 1.8e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07180 AT5G07180 AT5G62230 AT5G62230
fragaria_vesca FvH4_7g28290 FvH4_7g28290 FvH4_7g28290 FvH4_7g28290
malus_domestica MD02G1216900.v1.1 MD07G1259500.v1.1 MD17G1114200.v1.1 MD17G1114300.v1.1
prunus_persica Prupe.2G283600_v2.0.a1
pyrus_communis pycom05g04060 pycom07g23280 pycom15g04040
rosa_chinensis RchiOBHm_Chr1g0375261 RchiOBHm_Chr2g0167851 RchiOBHm_Chr4g0390421 RchiOBHm_Chr4g0390431 RchiOBHm_Chr4g0442901 RchiOBHm_Chr4g0442981 RchiOBHm_Chr7g0228991 RchiOBHm_Chr7g0239711
rosa_laevigata RLG00000005460 RLG00000005968 RLG00000005971 RLG00000005973 RLG00000005978 RLG00000008547 RLG00000010012 RLG00000010245 RLG00000026673 RLG00000030046 RLG00000031637
rosa_multiflora Rmu_co8166860.1_g000001 Rmu_co8410161.1_g000001 Rmu_sc0000554.1_g000034 Rmu_sc0001782.1_g000050 Rmu_sc0002096.1_g000036 Rmu_sc0003006.1_g000022 Rmu_sc0003701.1_g000003 Rmu_sc0005877.1_g000004 Rmu_sc0006329.1_g000003 Rmu_sc0006695.1_g000032 Rmu_sc0008108.1_g000003 Rmu_sc0009414.1_g000003 Rmu_sc0010356.1_g000001 Rmu_sc0014119.1_g000002 Rmu_sc0014119.1_g000003 Rmu_sc0014161.1_g000004 Rmu_sc0023009.1_g000001 Rmu_sc0036835.1_g000001 Rmu_ssc0000204.1_g000009
rosa_roxburghii Rroxscaffold_1G00024810 Rroxscaffold_2G00110800 Rroxscaffold_2G00122430 Rroxscaffold_3G00261280 Rroxscaffold_4G00282890 Rroxscaffold_4G00330910 Rroxscaffold_5G00370350 Rroxscaffold_7G00161820
rosa_rugosa Rorug01G0389600 Rorug01G0389700 Rorug01G0389800 Rorug01G0389900 Rorug01G0389900 Rorug01G0390000 Rorug04G0343700 Rorug07G0155300
rosa_samantha Rh1AG400300 Rh1CG377300 Rh1DG394700 Rh2AG367100 Rh2BG612000 Rh2CG350500 Rh2DG389600 Rh3CG058100 Rh4AG396700 Rh4AG397100 Rh4AG397500 Rh4AG397600 Rh4AG397700 Rh4BG003000 Rh4BG127700 Rh4BG370500 Rh4BG393400 Rh4BG409200 Rh4CG035400 Rh4CG166700 Rh4CG408200 Rh4CG424700 Rh4DG028000 Rh4DG028100 Rh4DG403300 Rh4DG403800 Rh6DG080900 Rh7DG202700
rosa_wichuraiana Rw1G035540 Rw4G032760 Rw4G034230 Rw4G034240 Rw4G034260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2672
Acc36I ACCTGC 2 cut(s) 978, 2672
AccB7I CCANNNNNTGG 1 cut(s) 293
AccBSI CCGCTC 1 cut(s) 1407
AciI CCGC 7 cut(s) 273, 1407, 1535, 1700, 2329, 2615, 2837
AclWI GGATC 6 cut(s) 706, 742, 1203, 1216, 1879, 2471
AcuI CTGAAG 2 cut(s) 1258, 2670
AfaI GTAC 6 cut(s) 605, 677, 736, 1647, 2015, 2025
AfeI AGCGCT 1 cut(s) 639
AfiI CCNNNNNNNGG 8 cut(s) 238, 293, 938, 1010, 1755, 1918, 2044, 2736
AflII CTTAAG 1 cut(s) 446
AflIII ACRYGT 1 cut(s) 2264
AgeI ACCGGT 1 cut(s) 2067
AhdI GACNNNNNGTC 1 cut(s) 2581
AjnI CCWGG 4 cut(s) 750, 1072, 1754, 2169
AloI GAACNNNNNNTCC 2 cut(s) 299, 331
Alw21I GWGCWC 2 cut(s) 1386, 2717
Alw26I GTCTC 9 cut(s) 2, 69, 414, 1601, 2090, 2169, 2214, 2630, 2776
Alw44I GTGCAC 1 cut(s) 2713
AlwI GGATC 6 cut(s) 706, 742, 1203, 1216, 1879, 2471
AlwNI CAGNNNCTG 1 cut(s) 1716
Ama87I CYCGRG 1 cut(s) 1770
Aor51HI AGCGCT 1 cut(s) 639
AoxI GGCC 6 cut(s) 132, 240, 706, 920, 1351, 2738
ApaLI GTGCAC 1 cut(s) 2713
ApeKI GCWGC 2 cut(s) 1775, 2288
ArsI GACNNNNNNTTYG 5 cut(s) 33, 1085, 1117, 1384, 1416
AseI ATTAAT 2 cut(s) 1287, 1862
AsiGI ACCGGT 1 cut(s) 2067
Asp700I GAANNNNTTC 1 cut(s) 1178
AspLEI GCGC 1 cut(s) 640
AspS9I GGNCC 9 cut(s) 491, 920, 1093, 1136, 1352, 1424, 1447, 1892, 2231
AsuC2I CCSGG 1 cut(s) 356
AsuHPI GGTGA 6 cut(s) 575, 1088, 1668, 2303, 2653, 2945
AvaI CYCGRG 1 cut(s) 1770
AvaII GGWCC 7 cut(s) 491, 1093, 1136, 1424, 1447, 1892, 2231
AxyI CCTNAGG 2 cut(s) 858, 1451
BaeGI GKGCMC 1 cut(s) 2717
BamHI GGATCC 1 cut(s) 1208
Bbv12I GWGCWC 2 cut(s) 1386, 2717
BbvI GCAGC 2 cut(s) 1787, 2300
BccI CCATC 6 cut(s) 115, 1148, 1415, 1861, 2242, 2426
BciT130I CCWGG 4 cut(s) 752, 1074, 1756, 2171
BciVI GTATCC 1 cut(s) 2082
BclI TGATCA 1 cut(s) 1579
BcnI CCSGG 1 cut(s) 356
BcoDI GTCTC 9 cut(s) 2, 69, 414, 1601, 2090, 2169, 2214, 2630, 2776
BfaI CTAG 3 cut(s) 938, 2004, 2490
BfmI CTRYAG 2 cut(s) 1235, 1434
BfoI RGCGCY 1 cut(s) 641
BfrI CTTAAG 1 cut(s) 446
BfuAI ACCTGC 2 cut(s) 978, 2672
BfuI GTATCC 1 cut(s) 2082
BglI GCCNNNNNGGC 2 cut(s) 122, 2887
BisI GCNGC 3 cut(s) 1536, 1776, 2289
BlpI GCTNAGC 1 cut(s) 1028
BlsI GCNGC 3 cut(s) 1537, 1777, 2290
Bme1390I CCNGG 5 cut(s) 356, 752, 1074, 1756, 2171
Bme18I GGWCC 7 cut(s) 491, 1093, 1136, 1424, 1447, 1892, 2231
BmeRI GACNNNNNGTC 1 cut(s) 2581
BmeT110I CYCGRG 1 cut(s) 1770
BmgT120I GGNCC 9 cut(s) 491, 920, 1093, 1136, 1352, 1424, 1447, 1892, 2231
BmiI GGNNCC 5 cut(s) 551, 1138, 1159, 1210, 1448
BmrFI CCNGG 5 cut(s) 356, 752, 1074, 1756, 2171
BmrI ACTGGG 4 cut(s) 966, 999, 2418, 2568
BmsI GCATC 4 cut(s) 166, 2450, 2602, 2899
BmuI ACTGGG 4 cut(s) 966, 999, 2418, 2568
BpmI CTGGAG 3 cut(s) 438, 654, 2104
Bpu10I CCTNAGC 1 cut(s) 1005
Bpu1102I GCTNAGC 1 cut(s) 1028
BpuEI CTTGAG 6 cut(s) 476, 1313, 1400, 1625, 2277, 2567
BpuMI CCSGG 1 cut(s) 356
Bsa29I ATCGAT 1 cut(s) 2475
BsaBI GATNNNNATC 1 cut(s) 125
BsaI GGTCTC 3 cut(s) 1601, 2214, 2776
BsaJI CCNNGG 8 cut(s) 292, 572, 607, 1755, 1818, 2169, 2170, 2610
BsaWI WCCGGW 1 cut(s) 2067
BsaXI ACNNNNNCTCC 4 cut(s) 985, 1015, 2192, 2222
Bsc4I CCNNNNNNNGG 8 cut(s) 238, 293, 938, 1010, 1755, 1918, 2044, 2736
Bse118I RCCGGY 1 cut(s) 2067
Bse21I CCTNAGG 2 cut(s) 858, 1451
Bse3DI GCAATG 2 cut(s) 151, 1689
Bse8I GATNNNNATC 1 cut(s) 125
BseBI CCWGG 4 cut(s) 752, 1074, 1756, 2171
BseCI ATCGAT 1 cut(s) 2475
BseDI CCNNGG 8 cut(s) 292, 572, 607, 1755, 1818, 2169, 2170, 2610
BseGI GGATG 5 cut(s) 364, 1140, 2350, 2377, 2418
BseJI GATNNNNATC 1 cut(s) 125
BseLI CCNNNNNNNGG 8 cut(s) 238, 293, 938, 1010, 1755, 1918, 2044, 2736
BseMI GCAATG 2 cut(s) 151, 1689
BseMII CTCAG 7 cut(s) 447, 849, 996, 1465, 1521, 1953, 2724
BseRI GAGGAG 3 cut(s) 20, 2171, 2768
BseSI GKGCMC 1 cut(s) 2717
BseXI GCAGC 2 cut(s) 1787, 2300
BsgI GTGCAG 3 cut(s) 1147, 1729, 2448
BshFI GGCC 6 cut(s) 134, 242, 708, 922, 1353, 2740
BshTI ACCGGT 1 cut(s) 2067
BshVI ATCGAT 1 cut(s) 2475
BsiHKAI GWGCWC 2 cut(s) 1386, 2717
BsiHKCI CYCGRG 1 cut(s) 1770
BsiSI CCGG 2 cut(s) 356, 2068
BslFI GGGAC 5 cut(s) 628, 1006, 1122, 1314, 1460
BslI CCNNNNNNNGG 8 cut(s) 238, 293, 938, 1010, 1755, 1918, 2044, 2736
BsmAI GTCTC 9 cut(s) 2, 69, 414, 1601, 2090, 2169, 2214, 2630, 2776
BsmBI CGTCTC 2 cut(s) 2, 2090
BsmFI GGGAC 5 cut(s) 628, 1006, 1122, 1314, 1460
BsmI GAATGC 1 cut(s) 1500
BsnI GGCC 6 cut(s) 134, 242, 708, 922, 1353, 2740
Bso31I GGTCTC 3 cut(s) 1601, 2214, 2776
BsoBI CYCGRG 1 cut(s) 1770
Bsp1286I GDGCHC 2 cut(s) 1386, 2717
Bsp1407I TGTACA 2 cut(s) 734, 2013
Bsp143I GATC 9 cut(s) 538, 711, 747, 771, 1126, 1208, 1579, 1871, 2476
Bsp1720I GCTNAGC 1 cut(s) 1028
Bsp19I CCATGG 1 cut(s) 1818
BspACI CCGC 7 cut(s) 273, 1407, 1535, 1700, 2329, 2615, 2837
BspANI GGCC 6 cut(s) 134, 242, 708, 922, 1353, 2740
BspCNI CTCAG 7 cut(s) 446, 850, 997, 1464, 1520, 1954, 2725
BspDI ATCGAT 1 cut(s) 2475
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 5 cut(s) 551, 1138, 1159, 1210, 1448
BspMAI CTGCAG 2 cut(s) 1239, 1438
BspMI ACCTGC 2 cut(s) 978, 2672
BspPI GGATC 6 cut(s) 706, 742, 1203, 1216, 1879, 2471
BspTI CTTAAG 1 cut(s) 446
BspTNI GGTCTC 3 cut(s) 1601, 2214, 2776
BsrBI CCGCTC 1 cut(s) 1407
BsrDI GCAATG 2 cut(s) 151, 1689
BsrFI RCCGGY 1 cut(s) 2067
BsrGI TGTACA 2 cut(s) 734, 2013
BssAI RCCGGY 1 cut(s) 2067
BssECI CCNNGG 8 cut(s) 292, 572, 607, 1755, 1818, 2169, 2170, 2610
BssMI GATC 9 cut(s) 538, 711, 747, 771, 1126, 1208, 1579, 1871, 2476
BssT1I CCWWGG 5 cut(s) 292, 572, 607, 1818, 2610
Bst2UI CCWGG 4 cut(s) 752, 1074, 1756, 2171
Bst4CI ACNGT 8 cut(s) 13, 374, 734, 1595, 2011, 2163, 2629, 2641
Bst6I CTCTTC 1 cut(s) 2189
BstAFI CTTAAG 1 cut(s) 446
BstAUI TGTACA 2 cut(s) 734, 2013
BstC8I GCNNGC 3 cut(s) 288, 879, 1434
BstDEI CTNAG 9 cut(s) 433, 858, 1005, 1028, 1451, 1507, 1962, 2733, 2924
BstDSI CCRYGG 1 cut(s) 1818
BstF5I GGATG 5 cut(s) 364, 1140, 2350, 2377, 2418
BstH2I RGCGCY 1 cut(s) 641
BstHHI GCGC 1 cut(s) 640
BstKTI GATC 9 cut(s) 541, 714, 750, 774, 1129, 1211, 1582, 1874, 2479
BstMAI GTCTC 9 cut(s) 2, 69, 414, 1601, 2090, 2169, 2214, 2630, 2776
BstMBI GATC 9 cut(s) 538, 711, 747, 771, 1126, 1208, 1579, 1871, 2476
BstMWI GCNNNNNNNGC 9 cut(s) 122, 131, 451, 1087, 2285, 2328, 2548, 2746, 2887
BstNI CCWGG 4 cut(s) 752, 1074, 1756, 2171
BstSCI CCNGG 5 cut(s) 354, 750, 1072, 1754, 2169
BstSFI CTRYAG 2 cut(s) 1235, 1434
BstSLI GKGCMC 1 cut(s) 2717
BstV1I GCAGC 2 cut(s) 1787, 2300
BstX2I RGATCY 3 cut(s) 747, 1208, 1871
BstXI CCANNNNNNTGG 2 cut(s) 560, 2082
BstYI RGATCY 3 cut(s) 747, 1208, 1871
Bsu15I ATCGAT 1 cut(s) 2475
Bsu36I CCTNAGG 2 cut(s) 858, 1451
BsuI GTATCC 1 cut(s) 2082
BsuRI GGCC 6 cut(s) 134, 242, 708, 922, 1353, 2740
BsuTUI ATCGAT 1 cut(s) 2475
BtgI CCRYGG 1 cut(s) 1818
BtsCI GGATG 5 cut(s) 364, 1140, 2350, 2377, 2418
BtsI GCAGTG 2 cut(s) 1164, 1717
BveI ACCTGC 2 cut(s) 978, 2672
Cac8I GCNNGC 3 cut(s) 288, 879, 1434
CaiI CAGNNNCTG 1 cut(s) 1716
CciI TCATGA 1 cut(s) 181
CfoI GCGC 1 cut(s) 640
Cfr10I RCCGGY 1 cut(s) 2067
Cfr13I GGNCC 9 cut(s) 491, 920, 1093, 1136, 1352, 1424, 1447, 1892, 2231
ClaI ATCGAT 1 cut(s) 2475
Csp6I GTAC 6 cut(s) 604, 676, 735, 1646, 2014, 2024
CspAI ACCGGT 1 cut(s) 2067
CspCI CAANNNNNGTGG 2 cut(s) 1546, 1581
CviQI GTAC 6 cut(s) 604, 676, 735, 1646, 2014, 2024
DdeI CTNAG 9 cut(s) 433, 858, 1005, 1028, 1451, 1507, 1962, 2733, 2924
DpnI GATC 9 cut(s) 540, 713, 749, 773, 1128, 1210, 1581, 1873, 2478
DpnII GATC 9 cut(s) 538, 711, 747, 771, 1126, 1208, 1579, 1871, 2476
DriI GACNNNNNGTC 1 cut(s) 2581
Eam1104I CTCTTC 1 cut(s) 2189
Eam1105I GACNNNNNGTC 1 cut(s) 2581
EarI CTCTTC 1 cut(s) 2189
EciI GGCGGA 1 cut(s) 2630
Eco130I CCWWGG 5 cut(s) 292, 572, 607, 1818, 2610
Eco147I AGGCCT 1 cut(s) 134
Eco31I GGTCTC 3 cut(s) 1601, 2214, 2776
Eco47I GGWCC 7 cut(s) 491, 1093, 1136, 1424, 1447, 1892, 2231
Eco47III AGCGCT 1 cut(s) 639
Eco57I CTGAAG 2 cut(s) 1258, 2670
Eco81I CCTNAGG 2 cut(s) 858, 1451
Eco88I CYCGRG 1 cut(s) 1770
EcoO109I RGGNCCY 3 cut(s) 1136, 1447, 1892
EcoRI GAATTC 1 cut(s) 1068
EcoRII CCWGG 4 cut(s) 750, 1072, 1754, 2169
EcoT14I CCWWGG 5 cut(s) 292, 572, 607, 1818, 2610
EcoT22I ATGCAT 2 cut(s) 2443, 2751
ErhI CCWWGG 5 cut(s) 292, 572, 607, 1818, 2610
Esp3I CGTCTC 2 cut(s) 2, 2090
FaqI GGGAC 5 cut(s) 628, 1006, 1122, 1314, 1460
FauI CCCGC 4 cut(s) 266, 1400, 1707, 2336
FbaI TGATCA 1 cut(s) 1579
Fnu4HI GCNGC 3 cut(s) 1536, 1776, 2289
FokI GGATG 5 cut(s) 371, 1127, 2357, 2384, 2405
Fsp4HI GCNGC 3 cut(s) 1536, 1776, 2289
FspBI CTAG 3 cut(s) 938, 2004, 2490
GlaI GCGC 1 cut(s) 639
GluI GCNGC 3 cut(s) 1536, 1776, 2289
GsuI CTGGAG 3 cut(s) 438, 654, 2104
HaeII RGCGCY 1 cut(s) 641
HaeIII GGCC 6 cut(s) 134, 242, 708, 922, 1353, 2740
HapII CCGG 2 cut(s) 356, 2068
HhaI GCGC 1 cut(s) 640
Hin6I GCGC 1 cut(s) 638
HinP1I GCGC 1 cut(s) 638
HincII GTYRAC 5 cut(s) 342, 486, 987, 2644, 2842
HindII GTYRAC 5 cut(s) 342, 486, 987, 2644, 2842
HindIII AAGCTT 4 cut(s) 443, 452, 739, 1025
HpaI GTTAAC 2 cut(s) 342, 2842
HpaII CCGG 2 cut(s) 356, 2068
HphI GGTGA 6 cut(s) 575, 1088, 1668, 2303, 2653, 2945
Hpy166II GTNNAC 9 cut(s) 342, 486, 987, 2014, 2024, 2575, 2644, 2715, 2842
Hpy188I TCNGA 9 cut(s) 403, 549, 771, 1254, 1510, 2398, 2518, 2601, 2908
Hpy8I GTNNAC 9 cut(s) 342, 486, 987, 2014, 2024, 2575, 2644, 2715, 2842
HpyAV CCTTC 6 cut(s) 101, 439, 1127, 1282, 1922, 2560
HpyCH4III ACNGT 8 cut(s) 13, 374, 734, 1595, 2011, 2163, 2629, 2641
HpyCH4IV ACGT 3 cut(s) 92, 1669, 2266
HpyF10VI GCNNNNNNNGC 9 cut(s) 122, 131, 451, 1087, 2285, 2328, 2548, 2746, 2887
HpyF3I CTNAG 9 cut(s) 433, 858, 1005, 1028, 1451, 1507, 1962, 2733, 2924
HpySE526I ACGT 3 cut(s) 92, 1669, 2266
HspAI GCGC 1 cut(s) 638
Ksp22I TGATCA 1 cut(s) 1579
KspAI GTTAAC 2 cut(s) 342, 2842
Kzo9I GATC 9 cut(s) 538, 711, 747, 771, 1126, 1208, 1579, 1871, 2476
LmnI GCTCC 3 cut(s) 635, 1497, 2285
Lsp1109I GCAGC 2 cut(s) 1787, 2300
LweI GCATC 4 cut(s) 166, 2450, 2602, 2899
MaeI CTAG 3 cut(s) 938, 2004, 2490
MaeII ACGT 3 cut(s) 92, 1669, 2266
MaeIII GTNAC 7 cut(s) 93, 218, 822, 977, 1712, 2468, 2659
MalI GATC 9 cut(s) 540, 713, 749, 773, 1128, 1210, 1581, 1873, 2478
MbiI CCGCTC 1 cut(s) 1407
MboI GATC 9 cut(s) 538, 711, 747, 771, 1126, 1208, 1579, 1871, 2476
MboII GAAGA 6 cut(s) 43, 200, 484, 2176, 2300, 2575
MfeI CAATTG 2 cut(s) 1520, 2463
MflI RGATCY 3 cut(s) 747, 1208, 1871
MhlI GDGCHC 2 cut(s) 1386, 2717
MlyI GAGTC 2 cut(s) 2052, 2591
MmeI TCCRAC 3 cut(s) 1869, 2263, 2610
Mph1103I ATGCAT 2 cut(s) 2443, 2751
MroXI GAANNNNTTC 1 cut(s) 1178
MslI CAYNNNNRTG 3 cut(s) 1922, 2343, 2666
MspA1I CMGCKG 1 cut(s) 1702
MspCI CTTAAG 1 cut(s) 446
MspI CCGG 2 cut(s) 356, 2068
MspR9I CCNGG 5 cut(s) 356, 752, 1074, 1756, 2171
MunI CAATTG 2 cut(s) 1520, 2463
Mva1269I GAATGC 1 cut(s) 1500
MvaI CCWGG 4 cut(s) 752, 1074, 1756, 2171
MwoI GCNNNNNNNGC 9 cut(s) 122, 131, 451, 1087, 2285, 2328, 2548, 2746, 2887
NciI CCSGG 1 cut(s) 356
NcoI CCATGG 1 cut(s) 1818
NdeII GATC 9 cut(s) 538, 711, 747, 771, 1126, 1208, 1579, 1871, 2476
NlaIV GGNNCC 5 cut(s) 551, 1138, 1159, 1210, 1448
NmeAIII GCCGAG 1 cut(s) 2479
NmuCI GTSAC 5 cut(s) 93, 218, 822, 1712, 2659
NsiI ATGCAT 2 cut(s) 2443, 2751
PagI TCATGA 1 cut(s) 181
PaqCI CACCTGC 1 cut(s) 2672
PasI CCCWGGG 1 cut(s) 2170
PceI AGGCCT 1 cut(s) 134
PctI GAATGC 1 cut(s) 1500
PdmI GAANNNNTTC 1 cut(s) 1178
PfeI GAWTC 9 cut(s) 265, 314, 784, 853, 1111, 1174, 1411, 1956, 1966
PflFI GACNNNGTC 2 cut(s) 224, 1890
PflMI CCANNNNNTGG 1 cut(s) 293
PfoI TCCNGGA 2 cut(s) 354, 750
PinAI ACCGGT 1 cut(s) 2067
PkrI GCNGC 3 cut(s) 1537, 1777, 2290
PleI GAGTC 2 cut(s) 2052, 2590
PpsI GAGTC 2 cut(s) 2052, 2590
PpuMI RGGWCCY 3 cut(s) 1136, 1447, 1892
PshBI ATTAAT 2 cut(s) 1287, 1862
Psp5II RGGWCCY 3 cut(s) 1136, 1447, 1892
Psp6I CCWGG 4 cut(s) 750, 1072, 1754, 2169
PspGI CCWGG 4 cut(s) 750, 1072, 1754, 2169
PspN4I GGNNCC 5 cut(s) 551, 1138, 1159, 1210, 1448
PspPI GGNCC 9 cut(s) 491, 920, 1093, 1136, 1352, 1424, 1447, 1892, 2231
PspPPI RGGWCCY 3 cut(s) 1136, 1447, 1892
PstI CTGCAG 2 cut(s) 1239, 1438
PstNI CAGNNNCTG 1 cut(s) 1716
PsuI RGATCY 3 cut(s) 747, 1208, 1871
PsyI GACNNNGTC 2 cut(s) 224, 1890
RsaI GTAC 6 cut(s) 605, 677, 736, 1647, 2015, 2025
RsaNI GTAC 6 cut(s) 604, 676, 735, 1646, 2014, 2024
RseI CAYNNNNRTG 3 cut(s) 1922, 2343, 2666
SatI GCNGC 3 cut(s) 1536, 1776, 2289
Sau3AI GATC 9 cut(s) 538, 711, 747, 771, 1126, 1208, 1579, 1871, 2476
Sau96I GGNCC 9 cut(s) 491, 920, 1093, 1136, 1352, 1424, 1447, 1892, 2231
SchI GAGTC 2 cut(s) 2052, 2591
ScrFI CCNGG 5 cut(s) 356, 752, 1074, 1756, 2171
SduI GDGCHC 2 cut(s) 1386, 2717
SfaNI GCATC 4 cut(s) 166, 2450, 2602, 2899
SfcI CTRYAG 2 cut(s) 1235, 1434
SinI GGWCC 7 cut(s) 491, 1093, 1136, 1424, 1447, 1892, 2231
SmiMI CAYNNNNRTG 3 cut(s) 1922, 2343, 2666
SmlI CTYRAG 7 cut(s) 446, 455, 1292, 1379, 1604, 2292, 2546
SmoI CTYRAG 7 cut(s) 446, 455, 1292, 1379, 1604, 2292, 2546
SseBI AGGCCT 1 cut(s) 134
SsiI CCGC 7 cut(s) 273, 1407, 1535, 1700, 2329, 2615, 2837
SspMI CTAG 3 cut(s) 938, 2004, 2490
StuI AGGCCT 1 cut(s) 134
StyD4I CCNGG 5 cut(s) 354, 750, 1072, 1754, 2169
StyI CCWWGG 5 cut(s) 292, 572, 607, 1818, 2610
TaaI ACNGT 8 cut(s) 13, 374, 734, 1595, 2011, 2163, 2629, 2641
TaiI ACGT 3 cut(s) 95, 1672, 2269
TaqI TCGA 5 cut(s) 1727, 2237, 2444, 2475, 2820
TatI WGTACW 3 cut(s) 734, 2013, 2023
TauI GCSGC 1 cut(s) 1538
TfiI GAWTC 9 cut(s) 265, 314, 784, 853, 1111, 1174, 1411, 1956, 1966
TseFI GTSAC 5 cut(s) 93, 218, 822, 1712, 2659
TseI GCWGC 2 cut(s) 1775, 2288
Tsp45I GTSAC 5 cut(s) 93, 218, 822, 1712, 2659
TspGWI ACGGA 1 cut(s) 2114
Tth111I GACNNNGTC 2 cut(s) 224, 1890
Van91I CCANNNNNTGG 1 cut(s) 293
Vha464I CTTAAG 1 cut(s) 446
VneI GTGCAC 1 cut(s) 2713
VpaK11BI GGWCC 7 cut(s) 491, 1093, 1136, 1424, 1447, 1892, 2231
VspI ATTAAT 2 cut(s) 1287, 1862
XcmI CCANNNNNNNNNTGG 3 cut(s) 160, 265, 1126
XmnI GAANNNNTTC 1 cut(s) 1178
XspI CTAG 3 cut(s) 938, 2004, 2490
Zsp2I ATGCAT 2 cut(s) 2443, 2751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.